Prupe.4G094600_v2.0.a1

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0029037
Physical Location & Seq
Reverse (-)
4771228 .. 4773801
2574 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G094600.1

Sequence Viewer

Length: 2298 bp
ATGGAAGGGATTTTCTTTCAGTCCTTACATGGGATGCCAATTATGCGACTGATCTCATTGCTAATAATAGTTTTAGAGCTAGTGCTTGTATTACCAATTAGCACATCATCAACAACAGCAGCAGCACATGATCATCAAGCCCTCCTGCCTCAGGCCTTGCTTGGCTGCTCTGACCGCTGTGGTAATCTCACAATTCCATATCCATTTGGCATGGCTCCAGGTTGCTACCGCGGCCCTGAGTTCTTCATCAACTGTACTACTACTCACGAAGACGAAAACCCGGTACCATATTTGACAAAGATATCAGGCAGTCCTTATGATCTCATTCAGCTCCGCGTCACTAACATCTCTCTTGAGGAGGGTGATATGCATGTTTTGCAGCCTGTCACTCGATCTTGCGGTGAAGATCAGAATGACACTGACGGTGTGCTCCTCCTGCCCCCTCCTTTCACCTTCTCTGAAGACAAAAACTATTTATTCGCCGTTGGATGTGGCATTGTTGCAGTATTTCAAGGTTCCCGTCCAGGCCAAGGCCTAGATGGAAACACTACCTTGCCCCATATAAATCGCTATTGCGTCACCTATTGTGACGACAATTCTGATGGAAATAACACTGACCCAGTAATTAACGACGACTCTTGCTCTGGCTTTGCCTGTTCCCTAGCTCAACTCCCTGGAGGAGTGCAAAATCTTACGGTGATGTTTACTCCCGTTAGTAATATTAGTCGAGCTCGACATAGAAAATGGTACACTAAGTACCCGTGCAGGTACGCCTTTCTTGTGGAACAAAGCAAGTTCACATTCTCCCCCGGAACGAGTTTTGAGCAACTCAACAACACAAGCCACCTTCCAGTGGTTGTCAATTGGGAAATCGGGGGTCATGGGACATGCGATGCTGCTCGAAAGAGCAAGAAGGACTTTGCTTGCAAGGGAAATAGCAAGTGTGTGGATTGGCCTAGTGATAACATCCATGGCTCTACAGGTTACATTTGCAAGTGCAAGAAAGGTTACCAAGGGAACCCATACCATCCAGATGGCTGCCAAGATATTGATGAGTGCAAGTCAAATCCCTGCCCTGTCGGAAAGTGCATAAATACACCTGGGAGTCACTCTTGTTTATGTCCCAATGGCACCAAAGAAAGAAGTTGCAAAGAGAAATCAAAGACCCTTCTCATGGTTATGGGTATCAGTCTAATCTTGGTTTTACTTGTTGGAAGTTCGAGTTACATATGTTGGGGAATGAAGCAACGAAAGTACATCAGACTCAAGGAAAAGTACTTTCGAGAAAATGGCGGTTTGTTGTTACAGCAACAGCTTGCTCATCATGGAGGTGAGGCAACCAGAATCTTTACTGCAGTGGAACTTGAAAAGGCTACAAACAATTACCATGATAGTAGAGTCCTTGGCAGAGGAGCTTATGGAGTAGTTTACAAAGGAATATTACCAGATAACAGAACTGTTGCCATAAAAAAGTCGAAAGTTGCTGCCCCAACTCAGAGTGAGCAGTTTGCTAATGAGTTGACTGTTCTTTCTCAAATCAATCATAAAAATGTGGTGAGGCTATTAGGCTGTTGTTTAGAGGCAGAAGTGCCTTTACTAGTTTACGAGTTCATCACCAATGACACTCTTTTTGAGCACCTTCATGGTAAACAAAAGAAAGGATCATCGTTTGTATGGGAATTACGTTTGAAGATCGCAGCAGAAACTGCTGGAGCACTAGCATACTTGCACTCCTCCACTTTCATGCAAATCATACACAGAGATGTGAAAGCTATGAACATACTATTAGATGATAATTATACAGCAAAAGTGTCAGACTTTGGAGCTTCAGTATTGATTCCTCTAGATCAAACTCAAATAAAAACATTGGTGCAAGGGACATTCGGATACTTAGACCCCGAATATCTTCATTCAAACCAACTAACAGAAAAGAGCGATGTTTATAGCTTTGGAGTTGTCCTAGCAGAGCTACTGACAAGAAAAGTGGCATTTTCTTATGATAGGCCCGAGGCAGAGAGAAGTCTAGCAAGACTTTTTGTTTGTGCAATGGAAGAAGAGCGCTTGGATCATATTCTTGATGGTGACATTGTCACTGAAGGAAATATTGAGACAGTAAGAAATGTGGCTCATCTCGCGAAAAAATGTTTAATGTTAAAAGGGGAGGAAAGACCTACAATGAAAGAAGTAGCTATAGAGCTAGAGGGAATTATGAGAACTATGGCAAACCACCATCCACAGGGAAACAATGTTCAATTGCGTCCTGAAGACCAGACTTCGAATTTTCTGGGGTCACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

766

Amino Acids

84.76

Weight (kDa)

6.35

Isoelectric Point (pI)

38.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029037)

Species Orthologous Gene IDs
prunus_persica Prupe.4G094500_v2.0.a1 Prupe.4G094600_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 756
Acc65I GGTACC 1 cut(s) 283
AccB1I GGYRCC 2 cut(s) 283, 1130
AccII CGCG 3 cut(s) 231, 336, 2135
AciI CCGC 6 cut(s) 175, 229, 231, 334, 399, 1293
AclWI GGATC 2 cut(s) 1669, 2073
AcsI RAATTY 1 cut(s) 2278
AcuI CTGAAG 4 cut(s) 480, 1812, 2115, 2283
AfaI GTAC 7 cut(s) 256, 285, 749, 758, 770, 1256, 1277
AfeI AGCGCT 1 cut(s) 2060
AfiI CCNNNNNNNGG 6 cut(s) 30, 151, 530, 853, 1174, 2236
AgsI TTSAA 5 cut(s) 512, 1367, 1690, 1914, 2252
AhlI ACTAGT 1 cut(s) 1597
AjnI CCWGG 4 cut(s) 217, 523, 673, 1099
AloI GAACNNNNNNTCC 2 cut(s) 2232, 2264
Alw21I GWGCWC 4 cut(s) 432, 733, 1638, 1717
Alw26I GTCTC 1 cut(s) 2102
AlwI GGATC 2 cut(s) 1669, 2073
AlwNI CAGNNNCTG 1 cut(s) 1706
Ama87I CYCGRG 1 cut(s) 2006
Aor51HI AGCGCT 1 cut(s) 2060
AoxI GGCC 6 cut(s) 153, 232, 526, 532, 953, 2003
ApeKI GCWGC 8 cut(s) 119, 122, 165, 379, 896, 1038, 1484, 1697
ApoI RAATTY 1 cut(s) 2278
ArsI GACNNNNNNTTYG 4 cut(s) 1613, 1645, 2022, 2054
Asp700I GAANNNNTTC 1 cut(s) 1905
Asp718I GGTACC 1 cut(s) 283
AspLEI GCGC 1 cut(s) 2061
AspS9I GGNCC 2 cut(s) 233, 2004
AsuC2I CCSGG 2 cut(s) 281, 810
AsuII TTCGAA 1 cut(s) 2276
AvaI CYCGRG 1 cut(s) 2006
AxyI CCTNAGG 1 cut(s) 150
BanI GGYRCC 2 cut(s) 283, 1130
BanII GRGCYC 1 cut(s) 733
BarI GAAGNNNNNNTAC 2 cut(s) 1578, 1610
BbsI GAAGAC 3 cut(s) 276, 468, 2271
Bbv12I GWGCWC 4 cut(s) 432, 733, 1638, 1717
BbvI GCAGC 8 cut(s) 131, 134, 152, 391, 883, 1025, 1471, 1709
BccI CCATC 6 cut(s) 533, 596, 1028, 1035, 2072, 2238
BceAI ACGGC 1 cut(s) 467
BciT130I CCWGG 4 cut(s) 219, 525, 675, 1101
BciVI GTATCC 1 cut(s) 1880
BclI TGATCA 1 cut(s) 130
BcnI CCSGG 2 cut(s) 281, 810
BcoDI GTCTC 1 cut(s) 2102
BcuI ACTAGT 1 cut(s) 1597
BfmI CTRYAG 3 cut(s) 978, 1353, 2190
BfoI RGCGCY 1 cut(s) 2062
BfuAI ACCTGC 1 cut(s) 756
BfuI GTATCC 1 cut(s) 1880
BisI GCNGC 9 cut(s) 120, 123, 166, 232, 380, 897, 1039, 1485, 1698
BlsI GCNGC 9 cut(s) 121, 124, 167, 233, 381, 898, 1040, 1486, 1699
BmcAI AGTACT 1 cut(s) 1277
Bme1390I CCNGG 6 cut(s) 219, 281, 525, 675, 810, 1101
BmeT110I CYCGRG 1 cut(s) 2006
BmgT120I GGNCC 2 cut(s) 233, 2004
BmiI GGNNCC 5 cut(s) 216, 285, 517, 1019, 1132
BmrFI CCNGG 6 cut(s) 219, 281, 525, 675, 810, 1101
BmrI ACTGGG 1 cut(s) 614
BmsI GCATC 2 cut(s) 24, 883
BmuI ACTGGG 1 cut(s) 614
BpiI GAAGAC 3 cut(s) 276, 468, 2271
BpmI CTGGAG 3 cut(s) 201, 696, 1731
Bpu14I TTCGAA 1 cut(s) 2276
BpuEI CTTGAG 2 cut(s) 374, 1250
BpuMI CCSGG 2 cut(s) 281, 810
BsaJI CCNNGG 9 cut(s) 229, 529, 673, 808, 970, 1012, 1100, 1402, 2007
BsaXI ACNNNNNCTCC 4 cut(s) 1715, 1745, 1815, 1845
Bsc4I CCNNNNNNNGG 6 cut(s) 30, 151, 530, 853, 1174, 2236
Bse1I ACTGG 2 cut(s) 620, 851
Bse21I CCTNAGG 1 cut(s) 150
Bse3DI GCAATG 2 cut(s) 56, 2052
BseBI CCWGG 4 cut(s) 219, 525, 675, 1101
BseDI CCNNGG 9 cut(s) 229, 529, 673, 808, 970, 1012, 1100, 1402, 2007
BseGI GGATG 5 cut(s) 39, 494, 966, 1027, 2230
BseLI CCNNNNNNNGG 6 cut(s) 30, 151, 530, 853, 1174, 2236
BseMI GCAATG 2 cut(s) 56, 2052
BseMII CTCAG 3 cut(s) 164, 228, 1508
BseNI ACTGG 2 cut(s) 620, 851
BseRI GAGGAG 5 cut(s) 371, 422, 693, 1425, 1723
BseXI GCAGC 8 cut(s) 131, 134, 152, 391, 883, 1025, 1471, 1709
BsgI GTGCAG 1 cut(s) 784
Bsh1236I CGCG 3 cut(s) 231, 336, 2135
BshFI GGCC 6 cut(s) 155, 234, 528, 534, 955, 2005
BshNI GGYRCC 2 cut(s) 283, 1130
BsiHKAI GWGCWC 4 cut(s) 432, 733, 1638, 1717
BsiHKCI CYCGRG 1 cut(s) 2006
BsiSI CCGG 2 cut(s) 281, 810
BslFI GGGAC 3 cut(s) 898, 1107, 1891
BslI CCNNNNNNNGG 6 cut(s) 30, 151, 530, 853, 1174, 2236
BsmAI GTCTC 1 cut(s) 2102
BsmFI GGGAC 3 cut(s) 898, 1107, 1891
BsnI GGCC 6 cut(s) 155, 234, 528, 534, 955, 2005
BsoBI CYCGRG 1 cut(s) 2006
Bsp119I TTCGAA 1 cut(s) 2276
Bsp1286I GDGCHC 4 cut(s) 432, 733, 1638, 1717
Bsp143I GATC 9 cut(s) 51, 130, 319, 392, 406, 1661, 1692, 1846, 2065
Bsp19I CCATGG 1 cut(s) 970
Bsp68I TCGCGA 1 cut(s) 2135
BspACI CCGC 6 cut(s) 175, 229, 231, 334, 399, 1293
BspANI GGCC 6 cut(s) 155, 234, 528, 534, 955, 2005
BspCNI CTCAG 3 cut(s) 163, 229, 1507
BspFNI CGCG 3 cut(s) 231, 336, 2135
BspLI GGNNCC 5 cut(s) 216, 285, 517, 1019, 1132
BspMAI CTGCAG 1 cut(s) 1357
BspMI ACCTGC 1 cut(s) 756
BspPI GGATC 2 cut(s) 1669, 2073
BspQI GCTCTTC 1 cut(s) 2049
BspT104I TTCGAA 1 cut(s) 2276
BspT107I GGYRCC 2 cut(s) 283, 1130
BsrDI GCAATG 2 cut(s) 56, 2052
BsrI ACTGG 2 cut(s) 620, 851
BssECI CCNNGG 9 cut(s) 229, 529, 673, 808, 970, 1012, 1100, 1402, 2007
BssMI GATC 9 cut(s) 51, 130, 319, 392, 406, 1661, 1692, 1846, 2065
BssT1I CCWWGG 4 cut(s) 529, 970, 1012, 1402
Bst2UI CCWGG 4 cut(s) 219, 525, 675, 1101
Bst4CI ACNGT 6 cut(s) 254, 425, 697, 1459, 1525, 2113
Bst6I CTCTTC 1 cut(s) 2049
BstAPI GCANNNNNTGC 2 cut(s) 376, 1706
BstBI TTCGAA 1 cut(s) 2276
BstC8I GCNNGC 2 cut(s) 925, 1317
BstDEI CTNAG 5 cut(s) 150, 237, 753, 1494, 1891
BstDSI CCRYGG 2 cut(s) 229, 970
BstEII GGTNACC 2 cut(s) 1007, 2289
BstENI CCTNNNNNAGG 1 cut(s) 149
BstF5I GGATG 5 cut(s) 39, 494, 966, 1027, 2230
BstFNI CGCG 3 cut(s) 231, 336, 2135
BstH2I RGCGCY 1 cut(s) 2062
BstHHI GCGC 1 cut(s) 2061
BstKTI GATC 9 cut(s) 54, 133, 322, 395, 409, 1664, 1695, 1849, 2068
BstMAI GTCTC 1 cut(s) 2102
BstMBI GATC 9 cut(s) 51, 130, 319, 392, 406, 1661, 1692, 1846, 2065
BstMWI GCNNNNNNNGC 7 cut(s) 43, 174, 231, 376, 436, 1706, 2132
BstNI CCWGG 4 cut(s) 219, 525, 675, 1101
BstNSI RCATGY 2 cut(s) 374, 891
BstPI GGTNACC 2 cut(s) 1007, 2289
BstSCI CCNGG 6 cut(s) 217, 279, 523, 673, 808, 1099
BstSFI CTRYAG 3 cut(s) 978, 1353, 2190
BstUI CGCG 3 cut(s) 231, 336, 2135
BstV1I GCAGC 8 cut(s) 131, 134, 152, 391, 883, 1025, 1471, 1709
BstV2I GAAGAC 3 cut(s) 276, 468, 2271
BstXI CCANNNNNNTGG 1 cut(s) 1034
Bsu36I CCTNAGG 1 cut(s) 150
BsuI GTATCC 1 cut(s) 1880
BsuRI GGCC 6 cut(s) 155, 234, 528, 534, 955, 2005
BtgI CCRYGG 2 cut(s) 229, 970
BtgZI GCGATG 2 cut(s) 906, 1950
BtsCI GGATG 5 cut(s) 39, 494, 966, 1027, 2230
BtsI GCAGTG 1 cut(s) 1362
BtsIMutI CAGTG 5 cut(s) 417, 612, 858, 1362, 2091
BtuMI TCGCGA 1 cut(s) 2135
BveI ACCTGC 1 cut(s) 756
Cac8I GCNNGC 2 cut(s) 925, 1317
CaiI CAGNNNCTG 1 cut(s) 1706
CfoI GCGC 1 cut(s) 2061
Cfr13I GGNCC 2 cut(s) 233, 2004
Cfr42I CCGCGG 1 cut(s) 232
CseI GACGC 3 cut(s) 325, 565, 2246
Csp6I GTAC 7 cut(s) 255, 284, 748, 757, 769, 1255, 1276
CspCI CAANNNNNGTGG 2 cut(s) 1965, 2000
CviQI GTAC 7 cut(s) 255, 284, 748, 757, 769, 1255, 1276
DdeI CTNAG 5 cut(s) 150, 237, 753, 1494, 1891
DpnI GATC 9 cut(s) 53, 132, 321, 394, 408, 1663, 1694, 1848, 2067
DpnII GATC 9 cut(s) 51, 130, 319, 392, 406, 1661, 1692, 1846, 2065
Eam1104I CTCTTC 1 cut(s) 2049
EarI CTCTTC 1 cut(s) 2049
Ecl136II GAGCTC 1 cut(s) 731
Eco130I CCWWGG 4 cut(s) 529, 970, 1012, 1402
Eco147I AGGCCT 2 cut(s) 155, 534
Eco24I GRGCYC 1 cut(s) 733
Eco32I GATATC 1 cut(s) 303
Eco47III AGCGCT 1 cut(s) 2060
Eco53kI GAGCTC 1 cut(s) 731
Eco57I CTGAAG 4 cut(s) 480, 1812, 2115, 2283
Eco81I CCTNAGG 1 cut(s) 150
Eco88I CYCGRG 1 cut(s) 2006
Eco91I GGTNACC 2 cut(s) 1007, 2289
EcoICRI GAGCTC 1 cut(s) 731
EcoNI CCTNNNNNAGG 1 cut(s) 149
EcoO65I GGTNACC 2 cut(s) 1007, 2289
EcoRII CCWGG 4 cut(s) 217, 523, 673, 1099
EcoRV GATATC 1 cut(s) 303
EcoT14I CCWWGG 4 cut(s) 529, 970, 1012, 1402
EcoT22I ATGCAT 1 cut(s) 372
EcoT38I GRGCYC 1 cut(s) 733
ErhI CCWWGG 4 cut(s) 529, 970, 1012, 1402
FalI AAGNNNNNCTT 2 cut(s) 902, 934
FaqI GGGAC 3 cut(s) 898, 1107, 1891
FauNDI CATATG 1 cut(s) 1229
FbaI TGATCA 1 cut(s) 130
Fnu4HI GCNGC 9 cut(s) 120, 123, 166, 232, 380, 897, 1039, 1485, 1698
FokI GGATG 5 cut(s) 46, 501, 953, 1014, 2217
FriOI GRGCYC 1 cut(s) 733
Fsp4HI GCNGC 9 cut(s) 120, 123, 166, 232, 380, 897, 1039, 1485, 1698
GlaI GCGC 1 cut(s) 2060
GluI GCNGC 9 cut(s) 120, 123, 166, 232, 380, 897, 1039, 1485, 1698
GsuI CTGGAG 3 cut(s) 201, 696, 1731
HaeII RGCGCY 1 cut(s) 2062
HaeIII GGCC 6 cut(s) 155, 234, 528, 534, 955, 2005
HapII CCGG 2 cut(s) 281, 810
HgaI GACGC 3 cut(s) 325, 565, 2246
HhaI GCGC 1 cut(s) 2061
Hin6I GCGC 1 cut(s) 2059
HinP1I GCGC 1 cut(s) 2059
HincII GTYRAC 1 cut(s) 1521
HindII GTYRAC 1 cut(s) 1521
HinfI GANTC 6 cut(s) 635, 1105, 1263, 1344, 1398, 1837
HpaII CCGG 2 cut(s) 281, 810
Hpy166II GTNNAC 7 cut(s) 705, 750, 798, 1429, 1521, 1603, 1649
Hpy188I TCNGA 9 cut(s) 172, 411, 460, 601, 1082, 1262, 1497, 1816, 1886
Hpy188III TCNNGA 8 cut(s) 266, 353, 1031, 1283, 1844, 2075, 2134, 2261
Hpy8I GTNNAC 7 cut(s) 705, 750, 798, 1429, 1521, 1603, 1649
Hpy99I CGWCG 1 cut(s) 635
HpyAV CCTTC 6 cut(s) 463, 857, 907, 1178, 1649, 2090
HpyCH4III ACNGT 6 cut(s) 254, 425, 697, 1459, 1525, 2113
HpyCH4IV ACGT 1 cut(s) 1684
HpyF10VI GCNNNNNNNGC 7 cut(s) 43, 174, 231, 376, 436, 1706, 2132
HpyF3I CTNAG 5 cut(s) 150, 237, 753, 1494, 1891
HpySE526I ACGT 1 cut(s) 1684
HspAI GCGC 1 cut(s) 2059
KpnI GGTACC 1 cut(s) 287
Ksp22I TGATCA 1 cut(s) 130
KspI CCGCGG 1 cut(s) 232
Kzo9I GATC 9 cut(s) 51, 130, 319, 392, 406, 1661, 1692, 1846, 2065
LguI GCTCTTC 1 cut(s) 2049
LmnI GCTCC 6 cut(s) 220, 336, 435, 1412, 1712, 1823
Lsp1109I GCAGC 8 cut(s) 131, 134, 152, 391, 883, 1025, 1471, 1709
LweI GCATC 2 cut(s) 24, 883
MaeII ACGT 1 cut(s) 1684
MalI GATC 9 cut(s) 53, 132, 321, 394, 408, 1663, 1694, 1848, 2067
MboI GATC 9 cut(s) 51, 130, 319, 392, 406, 1661, 1692, 1846, 2065
MboII GAAGA 9 cut(s) 235, 281, 416, 473, 1702, 1898, 2063, 2066, 2276
MfeI CAATTG 2 cut(s) 862, 2252
MhlI GDGCHC 4 cut(s) 432, 733, 1638, 1717
MlyI GAGTC 4 cut(s) 629, 1114, 1257, 1407
MmeI TCCRAC 3 cut(s) 466, 1060, 1192
Mph1103I ATGCAT 1 cut(s) 372
MroXI GAANNNNTTC 1 cut(s) 1905
MseI TTAA 3 cut(s) 627, 2147, 2153
MslI CAYNNNNRTG 7 cut(s) 1032, 1178, 1329, 1548, 1641, 1742, 1761
MspA1I CMGCKG 2 cut(s) 177, 231
MspI CCGG 2 cut(s) 281, 810
MspR9I CCNGG 6 cut(s) 219, 281, 525, 675, 810, 1101
MunI CAATTG 2 cut(s) 862, 2252
MvaI CCWGG 4 cut(s) 219, 525, 675, 1101
MvnI CGCG 3 cut(s) 231, 336, 2135
MwoI GCNNNNNNNGC 7 cut(s) 43, 174, 231, 376, 436, 1706, 2132
NciI CCSGG 2 cut(s) 281, 810
NcoI CCATGG 1 cut(s) 970
NdeI CATATG 1 cut(s) 1229
NdeII GATC 9 cut(s) 51, 130, 319, 392, 406, 1661, 1692, 1846, 2065
NlaIV GGNNCC 5 cut(s) 216, 285, 517, 1019, 1132
NmuCI GTSAC 8 cut(s) 337, 385, 577, 587, 1106, 2081, 2089, 2289
NruI TCGCGA 1 cut(s) 2135
NsiI ATGCAT 1 cut(s) 372
NspI RCATGY 2 cut(s) 374, 891
NspV TTCGAA 1 cut(s) 2276
PceI AGGCCT 2 cut(s) 155, 534
PciSI GCTCTTC 1 cut(s) 2049
PdmI GAANNNNTTC 1 cut(s) 1905
PfeI GAWTC 2 cut(s) 1344, 1837
PflFI GACNNNGTC 1 cut(s) 2087
PkrI GCNGC 9 cut(s) 121, 124, 167, 233, 381, 898, 1040, 1486, 1699
PleI GAGTC 4 cut(s) 629, 1113, 1257, 1406
PpsI GAGTC 4 cut(s) 629, 1113, 1257, 1406
Psp124BI GAGCTC 1 cut(s) 733
Psp6I CCWGG 4 cut(s) 217, 523, 673, 1099
PspEI GGTNACC 2 cut(s) 1007, 2289
PspGI CCWGG 4 cut(s) 217, 523, 673, 1099
PspN4I GGNNCC 5 cut(s) 216, 285, 517, 1019, 1132
PspPI GGNCC 2 cut(s) 233, 2004
PstI CTGCAG 1 cut(s) 1357
PstNI CAGNNNCTG 1 cut(s) 1706
PsyI GACNNNGTC 1 cut(s) 2087
RruI TCGCGA 1 cut(s) 2135
RsaI GTAC 7 cut(s) 256, 285, 749, 758, 770, 1256, 1277
RsaNI GTAC 7 cut(s) 255, 284, 748, 757, 769, 1255, 1276
RseI CAYNNNNRTG 7 cut(s) 1032, 1178, 1329, 1548, 1641, 1742, 1761
SacI GAGCTC 1 cut(s) 733
SacII CCGCGG 1 cut(s) 232
SapI GCTCTTC 1 cut(s) 2049
SaqAI TTAA 3 cut(s) 627, 2147, 2153
SatI GCNGC 9 cut(s) 120, 123, 166, 232, 380, 897, 1039, 1485, 1698
Sau3AI GATC 9 cut(s) 51, 130, 319, 392, 406, 1661, 1692, 1846, 2065
Sau96I GGNCC 2 cut(s) 233, 2004
ScaI AGTACT 1 cut(s) 1277
SchI GAGTC 4 cut(s) 629, 1114, 1257, 1407
ScrFI CCNGG 6 cut(s) 219, 281, 525, 675, 810, 1101
SduI GDGCHC 4 cut(s) 432, 733, 1638, 1717
SfaNI GCATC 2 cut(s) 24, 883
SfcI CTRYAG 3 cut(s) 978, 1353, 2190
Sfr303I CCGCGG 1 cut(s) 232
SfuI TTCGAA 1 cut(s) 2276
SgrBI CCGCGG 1 cut(s) 232
SmiMI CAYNNNNRTG 7 cut(s) 1032, 1178, 1329, 1548, 1641, 1742, 1761
SmlI CTYRAG 2 cut(s) 353, 1265
SmoI CTYRAG 2 cut(s) 353, 1265
SpeI ACTAGT 1 cut(s) 1597
SseBI AGGCCT 2 cut(s) 155, 534
SsiI CCGC 6 cut(s) 175, 229, 231, 334, 399, 1293
SspI AATATT 3 cut(s) 721, 1440, 2104
SstI GAGCTC 1 cut(s) 733
StuI AGGCCT 2 cut(s) 155, 534
StyD4I CCNGG 6 cut(s) 217, 279, 523, 673, 808, 1099
StyI CCWWGG 4 cut(s) 529, 970, 1012, 1402
TaaI ACNGT 6 cut(s) 254, 425, 697, 1459, 1525, 2113
TaiI ACGT 1 cut(s) 1687
TaqI TCGA 8 cut(s) 391, 727, 733, 901, 1220, 1282, 1475, 2276
TatI WGTACW 3 cut(s) 254, 1254, 1275
TauI GCSGC 1 cut(s) 234
TfiI GAWTC 2 cut(s) 1344, 1837
Tru1I TTAA 3 cut(s) 627, 2147, 2153
Tru9I TTAA 3 cut(s) 627, 2147, 2153
TscAI CASTG 5 cut(s) 424, 619, 858, 1362, 2098
TseFI GTSAC 8 cut(s) 337, 385, 577, 587, 1106, 2081, 2089, 2289
TseI GCWGC 8 cut(s) 119, 122, 165, 379, 896, 1038, 1484, 1697
Tsp45I GTSAC 8 cut(s) 337, 385, 577, 587, 1106, 2081, 2089, 2289
TspDTI ATGAA 8 cut(s) 235, 1256, 1600, 1631, 1732, 1790, 1898, 2192
TspRI CASTG 5 cut(s) 424, 619, 858, 1362, 2098
Tth111I GACNNNGTC 1 cut(s) 2087
XagI CCTNNNNNAGG 1 cut(s) 149
XapI RAATTY 1 cut(s) 2278
XbaI TCTAGA 1 cut(s) 1843
XceI RCATGY 2 cut(s) 374, 891
XcmI CCANNNNNNNNNTGG 1 cut(s) 536
XmnI GAANNNNTTC 1 cut(s) 1905
ZrmI AGTACT 1 cut(s) 1277
Zsp2I ATGCAT 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.