Prupe.4G166800_v2.0.a1

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0024490
Physical Location & Seq
Forward (+)
9691144 .. 9692688
1545 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G166800.1

Sequence Viewer

Length: 1344 bp
ATGCCTGATACAAAGGCTATTTTGCCTGCGATAGCCACTGTTGCTGCTTCTGCAATGCTTCTTAGAAGTATTGCCAATGACTTCATTCCTAATGAGTTTCGAAACTACTTCTTCTCAAGCCTCCATAACATTTCCCGGCGCTTCTCTTCTCACCTCACCATTGTAATAGATGAGTTTCAAGGGCTGTCCTTGAATGAAGTCTTTGAGGCTGCTGAAGCCTATTTGGGCACTTTAGCAACCCCGTCTCTTCAAAGAATCAAAGTGAGTAAGGTTACCAAGGAGAAGAAGCTCGGAGTTGCGATGGATCGAGGGGAAGAGATACGTGACGTTTATGAAGATCATGTTCAATTGATGTGGAAATTTGTTTGTACAAAGGTTGAATCATCAAGGGTTACAAATCCTGGTGATCTTAATGCATCCTTAAGAGCAGAAACAAGATCCTATGAGCTAACTTTCCACAAGAAGCACAAGGAGAAGGTATTAAATTCATACCTTCCATACATTTTAGACAGATCAAAGGAGATTAAAGTAGAACGAAAGGCAGTAAAGCTGTGCACAATTGATCAATATGAATGTTATGGGACCCAAGAGGTAAATCTGAATCACCCCATGACTTTTGAGCTGCTTGCCATGGACATGGAGATTAAGAAGGTTTTGTTGGATGATTTGAATAATTTCAAAAATGGAAAGGAATTTTACAGAAGGATCGGAAAAGCTTGGAAACGTGGGTACTTGTTATATGGCCCTCCTGGCACAGGCAAGTCCAGCTTGATTGCAGCCATCGCTAATCACCTTAACTATGACATCTACGAGTTGGAGCTAGCAGATGTTGGTTCCAATTCCGATTTGAAGAGAAGGTTGCTTGCCATGCCTGACCAATCTATACTTGTAATCGAAGATATCGATTGCACAATTAACCTGCAAAACCGGGAAACCGAGAATGAAGCACCGAGTACGGGGAAAAATCAGGTGACACTCTCTGGGCTCCTCAACCTCATTGATGGTCTTTGGTCATGTTGTACTGACGAACGCATAATAATTTTCACAACAAATCACAAAGAGAAGCTAGATCCTGCCTTAATGAGACCTGGACGCATGGACATGCACATAAACATGTCATATTGCACTCTTTCTGCATTCAAGCAGCTGGCATTCAATTACCATGGCCTCTCCCATCACCAACTCTTCGAGCAGATCGAAGGACTTATAGGGGAGATTAAAGTCACCCCAGCAGAAGTTGCAGGGGAGTTGATGAAGAGCAGAGATGCTCAAACTTCACTCCAAGGCCTCATCAATTTCCTCCTGGAGAAGAAAAATCAGCAAGAGATTGTGAGGCCAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_assoc

Protein Analysis

448

Amino Acids

50.87

Weight (kDa)

6.48

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024490)

Species Orthologous Gene IDs
malus_domestica MD11G1261500.v1.1
prunus_persica Prupe.4G166800_v2.0.a1
pyrus_communis pycom11g23070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 927
AclWI GGATC 4 cut(s) 312, 432, 713, 1064
AcsI RAATTY 3 cut(s) 359, 484, 692
AcuI CTGAAG 1 cut(s) 234
AfaI GTAC 4 cut(s) 370, 731, 955, 1021
AfiI CCNNNNNNNGG 2 cut(s) 755, 956
AflII CTTAAG 1 cut(s) 421
AflIII ACRYGT 1 cut(s) 1113
AjnI CCWGG 4 cut(s) 400, 748, 1087, 1302
AjuI GAANNNNNNNTTGG 2 cut(s) 641, 673
AluBI AGCT 9 cut(s) 289, 448, 550, 622, 716, 768, 820, 1066, 1147
AluI AGCT 9 cut(s) 289, 448, 550, 622, 716, 768, 820, 1066, 1147
Alw21I GWGCWC 1 cut(s) 557
Alw26I GTCTC 2 cut(s) 249, 1078
Alw44I GTGCAC 1 cut(s) 553
AlwI GGATC 4 cut(s) 312, 432, 713, 1064
AoxI GGCC 4 cut(s) 742, 1165, 1285, 1334
ApaLI GTGCAC 1 cut(s) 553
ApeKI GCWGC 5 cut(s) 44, 209, 622, 776, 1144
ApoI RAATTY 3 cut(s) 359, 484, 692
Asp700I GAANNNNTTC 1 cut(s) 674
AspLEI GCGC 1 cut(s) 141
AspS9I GGNCC 2 cut(s) 582, 743
AsuC2I CCSGG 2 cut(s) 136, 929
AsuHPI GGTGA 8 cut(s) 143, 148, 416, 596, 782, 982, 1169, 1216
AsuII TTCGAA 1 cut(s) 100
AsuNHI GCTAGC 1 cut(s) 820
AvaII GGWCC 1 cut(s) 582
BaeGI GKGCMC 2 cut(s) 230, 557
BanII GRGCYC 1 cut(s) 987
Bbv12I GWGCWC 1 cut(s) 557
BbvI GCAGC 5 cut(s) 31, 196, 609, 788, 1156
BccI CCATC 4 cut(s) 295, 788, 995, 1182
BciT130I CCWGG 4 cut(s) 402, 750, 1089, 1304
BclI TGATCA 1 cut(s) 562
BcnI CCSGG 2 cut(s) 136, 929
BcoDI GTCTC 2 cut(s) 249, 1078
BfaI CTAG 2 cut(s) 821, 1067
BfoI RGCGCY 1 cut(s) 142
BfrI CTTAAG 1 cut(s) 421
BfuAI ACCTGC 1 cut(s) 927
BglI GCCNNNNNGGC 1 cut(s) 750
BisI GCNGC 5 cut(s) 45, 210, 623, 777, 1145
BlsI GCNGC 5 cut(s) 46, 211, 624, 778, 1146
Bme1390I CCNGG 6 cut(s) 136, 402, 750, 929, 1089, 1304
Bme18I GGWCC 1 cut(s) 582
BmgT120I GGNCC 2 cut(s) 582, 743
BmiI GGNNCC 4 cut(s) 583, 584, 835, 986
BmrFI CCNGG 6 cut(s) 136, 402, 750, 929, 1089, 1304
BmsI GCATC 2 cut(s) 425, 1255
BmtI GCTAGC 1 cut(s) 824
BpmI CTGGAG 1 cut(s) 1325
Bpu14I TTCGAA 1 cut(s) 100
BpuEI CTTGAG 1 cut(s) 100
BpuMI CCSGG 2 cut(s) 136, 929
Bsa29I ATCGAT 1 cut(s) 903
BsaAI YACGTR 1 cut(s) 323
BsaI GGTCTC 1 cut(s) 1078
BsaJI CCNNGG 4 cut(s) 276, 630, 1162, 1282
Bsc4I CCNNNNNNNGG 2 cut(s) 755, 956
Bse3DI GCAATG 1 cut(s) 60
BseBI CCWGG 4 cut(s) 402, 750, 1089, 1304
BseCI ATCGAT 1 cut(s) 903
BseDI CCNNGG 4 cut(s) 276, 630, 1162, 1282
BseGI GGATG 2 cut(s) 416, 667
BseLI CCNNNNNNNGG 2 cut(s) 755, 956
BseMI GCAATG 1 cut(s) 60
BseRI GAGGAG 1 cut(s) 977
BseSI GKGCMC 2 cut(s) 230, 557
BseXI GCAGC 5 cut(s) 31, 196, 609, 788, 1156
BseYI CCCAGC 1 cut(s) 1228
BshFI GGCC 4 cut(s) 744, 1167, 1287, 1336
BshVI ATCGAT 1 cut(s) 903
BsiHKAI GWGCWC 1 cut(s) 557
BsiSI CCGG 2 cut(s) 136, 928
BslFI GGGAC 1 cut(s) 595
BslI CCNNNNNNNGG 2 cut(s) 755, 956
BsmAI GTCTC 2 cut(s) 249, 1078
BsmBI CGTCTC 1 cut(s) 249
BsmFI GGGAC 1 cut(s) 595
BsmI GAATGC 2 cut(s) 1136, 1151
BsnI GGCC 4 cut(s) 744, 1167, 1287, 1336
Bso31I GGTCTC 1 cut(s) 1078
Bsp119I TTCGAA 1 cut(s) 100
Bsp1286I GDGCHC 3 cut(s) 230, 557, 987
Bsp1407I TGTACA 1 cut(s) 368
Bsp143I GATC 9 cut(s) 304, 337, 406, 437, 512, 562, 705, 1069, 1194
Bsp19I CCATGG 2 cut(s) 630, 1162
BspANI GGCC 4 cut(s) 744, 1167, 1287, 1336
BspDI ATCGAT 1 cut(s) 903
BspLI GGNNCC 4 cut(s) 583, 584, 835, 986
BspMI ACCTGC 1 cut(s) 927
BspOI GCTAGC 1 cut(s) 824
BspPI GGATC 4 cut(s) 312, 432, 713, 1064
BspQI GCTCTTC 1 cut(s) 1250
BspT104I TTCGAA 1 cut(s) 100
BspTI CTTAAG 1 cut(s) 421
BspTNI GGTCTC 1 cut(s) 1078
BsrDI GCAATG 1 cut(s) 60
BsrGI TGTACA 1 cut(s) 368
BssECI CCNNGG 4 cut(s) 276, 630, 1162, 1282
BssMI GATC 9 cut(s) 304, 337, 406, 437, 512, 562, 705, 1069, 1194
BssT1I CCWWGG 4 cut(s) 276, 630, 1162, 1282
Bst2UI CCWGG 4 cut(s) 402, 750, 1089, 1304
Bst4CI ACNGT 1 cut(s) 40
Bst6I CTCTTC 6 cut(s) 151, 252, 309, 845, 1190, 1250
BstAFI CTTAAG 1 cut(s) 421
BstAPI GCANNNNNTGC 1 cut(s) 1238
BstAUI TGTACA 1 cut(s) 368
BstBAI YACGTR 1 cut(s) 323
BstBI TTCGAA 1 cut(s) 100
BstC8I GCNNGC 5 cut(s) 27, 627, 822, 864, 1149
BstDEI CTNAG 1 cut(s) 62
BstDSI CCRYGG 2 cut(s) 630, 1162
BstEII GGTNACC 1 cut(s) 271
BstENI CCTNNNNNAGG 1 cut(s) 753
BstF5I GGATG 2 cut(s) 416, 667
BstH2I RGCGCY 1 cut(s) 142
BstHHI GCGC 1 cut(s) 141
BstKTI GATC 9 cut(s) 307, 340, 409, 440, 515, 565, 708, 1072, 1197
BstMAI GTCTC 2 cut(s) 249, 1078
BstMBI GATC 9 cut(s) 304, 337, 406, 437, 512, 562, 705, 1069, 1194
BstMWI GCNNNNNNNGC 8 cut(s) 41, 50, 215, 750, 765, 782, 868, 1238
BstNI CCWGG 4 cut(s) 402, 750, 1089, 1304
BstNSI RCATGY 2 cut(s) 1105, 1117
BstPI GGTNACC 1 cut(s) 271
BstSCI CCNGG 6 cut(s) 134, 400, 748, 927, 1087, 1302
BstSLI GKGCMC 2 cut(s) 230, 557
BstV1I GCAGC 5 cut(s) 31, 196, 609, 788, 1156
BstX2I RGATCY 2 cut(s) 437, 1069
BstXI CCANNNNNNTGG 1 cut(s) 637
BstYI RGATCY 2 cut(s) 437, 1069
Bsu15I ATCGAT 1 cut(s) 903
BsuRI GGCC 4 cut(s) 744, 1167, 1287, 1336
BsuTUI ATCGAT 1 cut(s) 903
BtgI CCRYGG 2 cut(s) 630, 1162
BtgZI GCGATG 2 cut(s) 314, 766
BtsCI GGATG 2 cut(s) 416, 667
BtsIMutI CAGTG 1 cut(s) 36
BveI ACCTGC 1 cut(s) 927
Cac8I GCNNGC 5 cut(s) 27, 627, 822, 864, 1149
CfoI GCGC 1 cut(s) 141
Cfr13I GGNCC 2 cut(s) 582, 743
ClaI ATCGAT 1 cut(s) 903
CseI GACGC 1 cut(s) 1101
Csp6I GTAC 4 cut(s) 369, 730, 954, 1020
CspCI CAANNNNNGTGG 2 cut(s) 335, 370
CviQI GTAC 4 cut(s) 369, 730, 954, 1020
DdeI CTNAG 1 cut(s) 62
DpnI GATC 9 cut(s) 306, 339, 408, 439, 514, 564, 707, 1071, 1196
DpnII GATC 9 cut(s) 304, 337, 406, 437, 512, 562, 705, 1069, 1194
Eam1104I CTCTTC 6 cut(s) 151, 252, 309, 845, 1190, 1250
EarI CTCTTC 6 cut(s) 151, 252, 309, 845, 1190, 1250
Eco130I CCWWGG 4 cut(s) 276, 630, 1162, 1282
Eco147I AGGCCT 1 cut(s) 1287
Eco24I GRGCYC 1 cut(s) 987
Eco31I GGTCTC 1 cut(s) 1078
Eco32I GATATC 1 cut(s) 901
Eco47I GGWCC 1 cut(s) 582
Eco57I CTGAAG 1 cut(s) 234
Eco91I GGTNACC 1 cut(s) 271
EcoNI CCTNNNNNAGG 1 cut(s) 753
EcoO109I RGGNCCY 1 cut(s) 582
EcoO65I GGTNACC 1 cut(s) 271
EcoRII CCWGG 4 cut(s) 400, 748, 1087, 1302
EcoRV GATATC 1 cut(s) 901
EcoT14I CCWWGG 4 cut(s) 276, 630, 1162, 1282
EcoT22I ATGCAT 1 cut(s) 418
EcoT38I GRGCYC 1 cut(s) 987
ErhI CCWWGG 4 cut(s) 276, 630, 1162, 1282
Esp3I CGTCTC 1 cut(s) 249
FalI AAGNNNNNCTT 2 cut(s) 752, 784
FaqI GGGAC 1 cut(s) 595
FbaI TGATCA 1 cut(s) 562
Fnu4HI GCNGC 5 cut(s) 45, 210, 623, 777, 1145
FokI GGATG 2 cut(s) 403, 674
FriOI GRGCYC 1 cut(s) 987
Fsp4HI GCNGC 5 cut(s) 45, 210, 623, 777, 1145
FspBI CTAG 2 cut(s) 821, 1067
GlaI GCGC 1 cut(s) 140
GluI GCNGC 5 cut(s) 45, 210, 623, 777, 1145
GsaI CCCAGC 1 cut(s) 1232
GsuI CTGGAG 1 cut(s) 1325
HaeII RGCGCY 1 cut(s) 142
HaeIII GGCC 4 cut(s) 744, 1167, 1287, 1336
HapII CCGG 2 cut(s) 136, 928
HgaI GACGC 1 cut(s) 1101
HhaI GCGC 1 cut(s) 141
Hin6I GCGC 1 cut(s) 139
HinP1I GCGC 1 cut(s) 139
HindIII AAGCTT 1 cut(s) 714
HinfI GANTC 3 cut(s) 255, 380, 601
HpaII CCGG 2 cut(s) 136, 928
HphI GGTGA 8 cut(s) 143, 148, 416, 596, 782, 982, 1169, 1216
Hpy166II GTNNAC 1 cut(s) 555
Hpy188I TCNGA 4 cut(s) 293, 600, 710, 844
Hpy8I GTNNAC 1 cut(s) 555
HpyAV CCTTC 6 cut(s) 469, 503, 643, 696, 849, 1193
HpyCH4III ACNGT 1 cut(s) 40
HpyCH4IV ACGT 3 cut(s) 322, 327, 724
HpyF10VI GCNNNNNNNGC 8 cut(s) 41, 50, 215, 750, 765, 782, 868, 1238
HpyF3I CTNAG 1 cut(s) 62
HpySE526I ACGT 3 cut(s) 322, 327, 724
HspAI GCGC 1 cut(s) 139
KflI GGGWCCC 1 cut(s) 582
Ksp22I TGATCA 1 cut(s) 562
Kzo9I GATC 9 cut(s) 304, 337, 406, 437, 512, 562, 705, 1069, 1194
LguI GCTCTTC 1 cut(s) 1250
LmnI GCTCC 2 cut(s) 817, 990
Lsp1109I GCAGC 5 cut(s) 31, 196, 609, 788, 1156
LweI GCATC 2 cut(s) 425, 1255
MaeI CTAG 2 cut(s) 821, 1067
MaeII ACGT 3 cut(s) 322, 327, 724
MaeIII GTNAC 5 cut(s) 271, 323, 391, 970, 1222
MalI GATC 9 cut(s) 306, 339, 408, 439, 514, 564, 707, 1071, 1196
MboI GATC 9 cut(s) 304, 337, 406, 437, 512, 562, 705, 1069, 1194
MfeI CAATTG 2 cut(s) 347, 558
MflI RGATCY 2 cut(s) 437, 1069
MhlI GDGCHC 3 cut(s) 230, 557, 987
MmeI TCCRAC 2 cut(s) 639, 795
Mph1103I ATGCAT 1 cut(s) 418
MroXI GAANNNNTTC 1 cut(s) 674
MseI TTAA 9 cut(s) 411, 422, 482, 525, 645, 795, 915, 1079, 1218
MslI CAYNNNNRTG 3 cut(s) 635, 1100, 1112
MspA1I CMGCKG 1 cut(s) 1147
MspCI CTTAAG 1 cut(s) 421
MspI CCGG 2 cut(s) 136, 928
MspR9I CCNGG 6 cut(s) 136, 402, 750, 929, 1089, 1304
MunI CAATTG 2 cut(s) 347, 558
Mva1269I GAATGC 2 cut(s) 1136, 1151
MvaI CCWGG 4 cut(s) 402, 750, 1089, 1304
MwoI GCNNNNNNNGC 8 cut(s) 41, 50, 215, 750, 765, 782, 868, 1238
NciI CCSGG 2 cut(s) 136, 929
NcoI CCATGG 2 cut(s) 630, 1162
NdeII GATC 9 cut(s) 304, 337, 406, 437, 512, 562, 705, 1069, 1194
NheI GCTAGC 1 cut(s) 820
NlaIV GGNNCC 4 cut(s) 583, 584, 835, 986
NmuCI GTSAC 3 cut(s) 323, 970, 1222
NsiI ATGCAT 1 cut(s) 418
NspI RCATGY 2 cut(s) 1105, 1117
NspV TTCGAA 1 cut(s) 100
PceI AGGCCT 1 cut(s) 1287
PciI ACATGT 1 cut(s) 1113
PciSI GCTCTTC 1 cut(s) 1250
PcsI WCGNNNNNNNCGW 2 cut(s) 900, 1194
PctI GAATGC 2 cut(s) 1136, 1151
PdmI GAANNNNTTC 1 cut(s) 674
PfeI GAWTC 3 cut(s) 255, 380, 601
PfoI TCCNGGA 1 cut(s) 1302
PkrI GCNGC 5 cut(s) 46, 211, 624, 778, 1146
Ppu21I YACGTR 1 cut(s) 323
PpuMI RGGWCCY 1 cut(s) 582
PscI ACATGT 1 cut(s) 1113
Psp5II RGGWCCY 1 cut(s) 582
Psp6I CCWGG 4 cut(s) 400, 748, 1087, 1302
PspEI GGTNACC 1 cut(s) 271
PspFI CCCAGC 1 cut(s) 1228
PspGI CCWGG 4 cut(s) 400, 748, 1087, 1302
PspN4I GGNNCC 4 cut(s) 583, 584, 835, 986
PspPI GGNCC 2 cut(s) 582, 743
PspPPI RGGWCCY 1 cut(s) 582
PsuI RGATCY 2 cut(s) 437, 1069
PvuII CAGCTG 1 cut(s) 1147
RsaI GTAC 4 cut(s) 370, 731, 955, 1021
RsaNI GTAC 4 cut(s) 369, 730, 954, 1020
RseI CAYNNNNRTG 3 cut(s) 635, 1100, 1112
SapI GCTCTTC 1 cut(s) 1250
SaqAI TTAA 9 cut(s) 411, 422, 482, 525, 645, 795, 915, 1079, 1218
SatI GCNGC 5 cut(s) 45, 210, 623, 777, 1145
Sau3AI GATC 9 cut(s) 304, 337, 406, 437, 512, 562, 705, 1069, 1194
Sau96I GGNCC 2 cut(s) 582, 743
ScrFI CCNGG 6 cut(s) 136, 402, 750, 929, 1089, 1304
SduI GDGCHC 3 cut(s) 230, 557, 987
SfaNI GCATC 2 cut(s) 425, 1255
SfuI TTCGAA 1 cut(s) 100
SinI GGWCC 1 cut(s) 582
SmiMI CAYNNNNRTG 3 cut(s) 635, 1100, 1112
SmlI CTYRAG 2 cut(s) 115, 421
SmoI CTYRAG 2 cut(s) 115, 421
SseBI AGGCCT 1 cut(s) 1287
SspMI CTAG 2 cut(s) 821, 1067
StuI AGGCCT 1 cut(s) 1287
StyD4I CCNGG 6 cut(s) 134, 400, 748, 927, 1087, 1302
StyI CCWWGG 4 cut(s) 276, 630, 1162, 1282
TaaI ACNGT 1 cut(s) 40
TaiI ACGT 3 cut(s) 325, 330, 727
TaqI TCGA 6 cut(s) 100, 307, 894, 903, 1188, 1197
TatI WGTACW 2 cut(s) 368, 1019
TfiI GAWTC 3 cut(s) 255, 380, 601
Tru1I TTAA 9 cut(s) 411, 422, 482, 525, 645, 795, 915, 1079, 1218
Tru9I TTAA 9 cut(s) 411, 422, 482, 525, 645, 795, 915, 1079, 1218
TscAI CASTG 1 cut(s) 43
TseFI GTSAC 3 cut(s) 323, 970, 1222
TseI GCWGC 5 cut(s) 44, 209, 622, 776, 1144
Tsp45I GTSAC 3 cut(s) 323, 970, 1222
TspDTI ATGAA 7 cut(s) 73, 210, 348, 477, 585, 957, 1268
TspRI CASTG 1 cut(s) 43
Vha464I CTTAAG 1 cut(s) 421
VneI GTGCAC 1 cut(s) 553
VpaK11BI GGWCC 1 cut(s) 582
XagI CCTNNNNNAGG 1 cut(s) 753
XapI RAATTY 3 cut(s) 359, 484, 692
XceI RCATGY 2 cut(s) 1105, 1117
XmnI GAANNNNTTC 1 cut(s) 674
XspI CTAG 2 cut(s) 821, 1067
Zsp2I ATGCAT 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.