Prupe.4G212800_v2.0.a1

Serine threonine-protein kinase PBS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0021232
Physical Location & Seq
Reverse (-)
13271695 .. 13275714
4020 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G212800.3

Sequence Viewer

Length: 1056 bp
ATGTTGTGTCCTACTCCTCCTCAGCAGCAGCAACCTCAATCTCTGCAAGCCTTGGCACGCAGCCAGTCCTTGTGTCTTCGACAATCTCAGTCTCCCCGGACTTCACAACGCTGCCGCTCTTTAAACTACAATTCAGGAAACGATGCAGATTACAGTCCGGATTTCTCAAACGCCACAATTGAATTTGTGATCAAAGGATTGAATAGTTTGAACATTAAGAAGAACCTCAGCAGGTTGCCTTTGCTCAGCAAAGGTTCGCATGATGACAACAGCAACCATTACAAGCAACAATACCAGACGACAGGATCTGACGATTCCAACCCCACCGAGTCGCTTTTACTGAGCGTTAGTCCCCATGCTGACACTGACCAAGACGAGCTGGGCAATGTAACAAGGGTAGTGTTTCAGGGCTCTGTAGATTCCGATCCGAGTGTGTCTCCTTTACCTTGCATTAGTACCCAAGCTGATACTGACTATGATGGGCGAGACAGTGCGGGATCTGATGACAACCTCAGTGACTCGCTTTTACCAAGCAGTATTCCTCGCATTAGCACCGACTATGACGAACCGGGCAATCCGGCAGTGGGCCCTTCTGGTTCAAATCCTAGTGAGTCTGCAGCAATTAACATTGGCTGGCAGGATGAACATAATGATTTTGGCCAGCCACATCACAGGACAAGGGTAACTGCCTTAGAGTTGACCTTTATGCTCGTTGGTTTCAACCTAGAAATATTGTCAGCCGGTTTCGATCAGGTTTCCTCCACAAGTAAGCCTCGTTATGCACTGATCAGTTTGCTGTTGGCCGTTGCGGCTGTGTTTACTTGCATCTGGGAGCTCATTTATAATGGTATGAAGGAGAGAGATGCATCTCCGAATAGCACGGTTTTTGGTACGTTGCCTGACATTTTCGGATTGGGACTTGCTGTGATTCAATGTGTTTGCTCGGCAGTGCAGTATTATTTCCTCCATCATCATCATAGTAATCCAATGAAACTGTCCCCTGTGCCTCTCTTTTTCTTCAGCTGTTTGGTGGTTTTGAAATTAAAAGGAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

352

Amino Acids

38.3

Weight (kDa)

5.08

Isoelectric Point (pI)

49.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 843
Acc36I ACCTGC 1 cut(s) 222
AccBSI CCGCTC 1 cut(s) 117
AccIII TCCGGA 1 cut(s) 157
AciI CCGC 3 cut(s) 115, 494, 809
AclWI GGATC 3 cut(s) 313, 419, 505
AcoI YGGCCR 2 cut(s) 658, 801
AcsI RAATTY 1 cut(s) 182
AcuI CTGAAG 1 cut(s) 1003
AfaI GTAC 2 cut(s) 457, 892
AfiI CCNNNNNNNGG 1 cut(s) 584
AgsI TTSAA 7 cut(s) 182, 202, 211, 600, 721, 932, 1039
AluBI AGCT 4 cut(s) 379, 464, 835, 1023
AluI AGCT 4 cut(s) 379, 464, 835, 1023
Alw21I GWGCWC 1 cut(s) 837
Alw26I GTCTC 3 cut(s) 96, 441, 480
AlwI GGATC 3 cut(s) 313, 419, 505
AlwNI CAGNNNCTG 1 cut(s) 308
Aor13HI TCCGGA 1 cut(s) 157
AoxI GGCC 3 cut(s) 586, 658, 801
ApaI GGGCCC 1 cut(s) 590
ApeKI GCWGC 5 cut(s) 25, 28, 60, 111, 617
ApoI RAATTY 1 cut(s) 182
AspS9I GGNCC 2 cut(s) 586, 587
AsuC2I CCSGG 2 cut(s) 97, 570
BaeGI GKGCMC 1 cut(s) 590
BalI TGGCCA 1 cut(s) 660
BanII GRGCYC 3 cut(s) 413, 590, 837
BbsI GAAGAC 1 cut(s) 68
Bbv12I GWGCWC 1 cut(s) 837
BbvCI CCTCAGC 2 cut(s) 21, 227
BbvI GCAGC 5 cut(s) 37, 40, 72, 98, 629
BccI CCATC 2 cut(s) 473, 975
BceAI ACGGC 1 cut(s) 788
BclI TGATCA 2 cut(s) 189, 786
BcnI CCSGG 2 cut(s) 97, 570
BcoDI GTCTC 3 cut(s) 96, 441, 480
BfaI CTAG 3 cut(s) 606, 725, 1054
BfmI CTRYAG 2 cut(s) 414, 615
BfuAI ACCTGC 1 cut(s) 222
BglI GCCNNNNNGGC 1 cut(s) 809
BisI GCNGC 7 cut(s) 26, 29, 61, 112, 115, 618, 810
BlpI GCTNAGC 1 cut(s) 245
BlsI GCNGC 7 cut(s) 27, 30, 62, 113, 116, 619, 811
Bme1390I CCNGG 2 cut(s) 97, 570
BmgT120I GGNCC 2 cut(s) 586, 587
BmiI GGNNCC 1 cut(s) 588
BmrFI CCNGG 2 cut(s) 97, 570
BmsI GCATC 4 cut(s) 133, 834, 853, 875
BpiI GAAGAC 1 cut(s) 68
BplI GAGNNNNNCTC 2 cut(s) 421, 453
Bpu10I CCTNAGC 2 cut(s) 21, 227
Bpu1102I GCTNAGC 1 cut(s) 245
BpuMI CCSGG 2 cut(s) 97, 570
BsaBI GATNNNNATC 1 cut(s) 423
BsaJI CCNNGG 2 cut(s) 51, 95
BsaWI WCCGGW 1 cut(s) 157
Bsc4I CCNNNNNNNGG 1 cut(s) 584
Bse118I RCCGGY 1 cut(s) 740
Bse1I ACTGG 1 cut(s) 64
Bse3DI GCAATG 1 cut(s) 391
Bse8I GATNNNNATC 1 cut(s) 423
BseAI TCCGGA 1 cut(s) 157
BseDI CCNNGG 2 cut(s) 51, 95
BseGI GGATG 1 cut(s) 646
BseJI GATNNNNATC 1 cut(s) 423
BseLI CCNNNNNNNGG 1 cut(s) 584
BseMI GCAATG 1 cut(s) 391
BseMII CTCAG 6 cut(s) 35, 101, 241, 259, 332, 526
BseNI ACTGG 1 cut(s) 64
BseRI GAGGAG 2 cut(s) 6, 9
BseSI GKGCMC 1 cut(s) 590
BseXI GCAGC 5 cut(s) 37, 40, 72, 98, 629
BseYI CCCAGC 1 cut(s) 379
BsgI GTGCAG 1 cut(s) 971
BshFI GGCC 3 cut(s) 588, 660, 803
BsiHKAI GWGCWC 1 cut(s) 837
BsiSI CCGG 5 cut(s) 97, 158, 569, 578, 741
BslFI GGGAC 3 cut(s) 336, 930, 982
BslI CCNNNNNNNGG 1 cut(s) 584
BsmAI GTCTC 3 cut(s) 96, 441, 480
BsmFI GGGAC 3 cut(s) 336, 930, 982
BsnI GGCC 3 cut(s) 588, 660, 803
Bsp120I GGGCCC 1 cut(s) 586
Bsp1286I GDGCHC 3 cut(s) 413, 590, 837
Bsp13I TCCGGA 1 cut(s) 157
Bsp143I GATC 6 cut(s) 189, 305, 424, 497, 748, 786
Bsp1720I GCTNAGC 1 cut(s) 245
BspACI CCGC 3 cut(s) 115, 494, 809
BspANI GGCC 3 cut(s) 588, 660, 803
BspCNI CTCAG 6 cut(s) 34, 100, 240, 258, 333, 525
BspEI TCCGGA 1 cut(s) 157
BspLI GGNNCC 1 cut(s) 588
BspMAI CTGCAG 1 cut(s) 619
BspMI ACCTGC 1 cut(s) 222
BspPI GGATC 3 cut(s) 313, 419, 505
BsrBI CCGCTC 1 cut(s) 117
BsrDI GCAATG 1 cut(s) 391
BsrFI RCCGGY 1 cut(s) 740
BsrI ACTGG 1 cut(s) 64
BssAI RCCGGY 1 cut(s) 740
BssECI CCNNGG 2 cut(s) 51, 95
BssMI GATC 6 cut(s) 189, 305, 424, 497, 748, 786
BssT1I CCWWGG 1 cut(s) 51
Bst4CI ACNGT 4 cut(s) 155, 491, 883, 996
BstC8I GCNNGC 4 cut(s) 48, 58, 635, 662
BstDEI CTNAG 7 cut(s) 21, 87, 227, 245, 341, 512, 691
BstF5I GGATG 1 cut(s) 646
BstKTI GATC 6 cut(s) 192, 308, 427, 500, 751, 789
BstMAI GTCTC 3 cut(s) 96, 441, 480
BstMBI GATC 6 cut(s) 189, 305, 424, 497, 748, 786
BstMWI GCNNNNNNNGC 1 cut(s) 809
BstSCI CCNGG 2 cut(s) 95, 568
BstSFI CTRYAG 2 cut(s) 414, 615
BstSLI GKGCMC 1 cut(s) 590
BstV1I GCAGC 5 cut(s) 37, 40, 72, 98, 629
BstV2I GAAGAC 1 cut(s) 68
BstX2I RGATCY 2 cut(s) 305, 497
BstYI RGATCY 2 cut(s) 305, 497
BsuRI GGCC 3 cut(s) 588, 660, 803
BtsCI GGATG 1 cut(s) 646
BtsI GCAGTG 2 cut(s) 588, 954
BtsIMutI CAGTG 6 cut(s) 363, 496, 520, 588, 782, 954
BveI ACCTGC 1 cut(s) 222
Cac8I GCNNGC 4 cut(s) 48, 58, 635, 662
CaiI CAGNNNCTG 1 cut(s) 308
Cfr10I RCCGGY 1 cut(s) 740
Cfr13I GGNCC 2 cut(s) 586, 587
Csp6I GTAC 2 cut(s) 456, 891
CviAII CATG 2 cut(s) 260, 356
CviQI GTAC 2 cut(s) 456, 891
DdeI CTNAG 7 cut(s) 21, 87, 227, 245, 341, 512, 691
DpnI GATC 6 cut(s) 191, 307, 426, 499, 750, 788
DpnII GATC 6 cut(s) 189, 305, 424, 497, 748, 786
DraI TTTAAA 1 cut(s) 123
EaeI YGGCCR 2 cut(s) 658, 801
Ecl136II GAGCTC 1 cut(s) 835
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 3 cut(s) 413, 590, 837
Eco53kI GAGCTC 1 cut(s) 835
Eco57I CTGAAG 1 cut(s) 1003
EcoICRI GAGCTC 1 cut(s) 835
EcoO109I RGGNCCY 1 cut(s) 587
EcoT14I CCWWGG 1 cut(s) 51
EcoT22I ATGCAT 1 cut(s) 868
EcoT38I GRGCYC 3 cut(s) 413, 590, 837
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 2 cut(s) 263, 359
FaqI GGGAC 3 cut(s) 336, 930, 982
FatI CATG 2 cut(s) 259, 355
FauI CCCGC 1 cut(s) 487
FbaI TGATCA 2 cut(s) 189, 786
Fnu4HI GCNGC 7 cut(s) 26, 29, 61, 112, 115, 618, 810
FokI GGATG 1 cut(s) 653
FriOI GRGCYC 3 cut(s) 413, 590, 837
Fsp4HI GCNGC 7 cut(s) 26, 29, 61, 112, 115, 618, 810
FspBI CTAG 3 cut(s) 606, 725, 1054
GluI GCNGC 7 cut(s) 26, 29, 61, 112, 115, 618, 810
GsaI CCCAGC 1 cut(s) 383
HaeIII GGCC 3 cut(s) 588, 660, 803
HapII CCGG 5 cut(s) 97, 158, 569, 578, 741
Hin1II CATG 2 cut(s) 263, 359
HincII GTYRAC 1 cut(s) 699
HindII GTYRAC 1 cut(s) 699
HinfI GANTC 6 cut(s) 314, 329, 419, 518, 611, 928
HpaII CCGG 5 cut(s) 97, 158, 569, 578, 741
Hpy166II GTNNAC 2 cut(s) 699, 819
Hpy188I TCNGA 6 cut(s) 310, 424, 429, 502, 873, 911
Hpy188III TCNNGA 2 cut(s) 135, 158
Hpy8I GTNNAC 2 cut(s) 699, 819
HpyAV CCTTC 2 cut(s) 600, 847
HpyCH4III ACNGT 4 cut(s) 155, 491, 883, 996
HpyCH4IV ACGT 1 cut(s) 893
HpyCH4V TGCA 8 cut(s) 46, 146, 450, 617, 782, 825, 866, 952
HpyF10VI GCNNNNNNNGC 1 cut(s) 809
HpyF3I CTNAG 7 cut(s) 21, 87, 227, 245, 341, 512, 691
HpySE526I ACGT 1 cut(s) 893
Hsp92II CATG 2 cut(s) 263, 359
Kpn2I TCCGGA 1 cut(s) 157
Ksp22I TGATCA 2 cut(s) 189, 786
Kzo9I GATC 6 cut(s) 189, 305, 424, 497, 748, 786
LmnI GCTCC 1 cut(s) 832
Lsp1109I GCAGC 5 cut(s) 37, 40, 72, 98, 629
LweI GCATC 4 cut(s) 133, 834, 853, 875
MaeI CTAG 3 cut(s) 606, 725, 1054
MaeII ACGT 1 cut(s) 893
MaeIII GTNAC 3 cut(s) 388, 515, 682
MalI GATC 6 cut(s) 191, 307, 426, 499, 750, 788
MbiI CCGCTC 1 cut(s) 117
MboI GATC 6 cut(s) 189, 305, 424, 497, 748, 786
MboII GAAGA 3 cut(s) 68, 232, 1009
MfeI CAATTG 1 cut(s) 177
MflI RGATCY 2 cut(s) 305, 497
MhlI GDGCHC 3 cut(s) 413, 590, 837
MlsI TGGCCA 1 cut(s) 660
MluCI AATT 5 cut(s) 130, 177, 182, 621, 1040
MluNI TGGCCA 1 cut(s) 660
MlyI GAGTC 3 cut(s) 338, 512, 620
MmeI TCCRAC 1 cut(s) 342
Mox20I TGGCCA 1 cut(s) 660
Mph1103I ATGCAT 1 cut(s) 868
MroI TCCGGA 1 cut(s) 157
MscI TGGCCA 1 cut(s) 660
MseI TTAA 4 cut(s) 122, 216, 624, 1043
Msp20I TGGCCA 1 cut(s) 660
MspA1I CMGCKG 1 cut(s) 1023
MspI CCGG 5 cut(s) 97, 158, 569, 578, 741
MspR9I CCNGG 2 cut(s) 97, 570
MunI CAATTG 1 cut(s) 177
MwoI GCNNNNNNNGC 1 cut(s) 809
NciI CCSGG 2 cut(s) 97, 570
NdeII GATC 6 cut(s) 189, 305, 424, 497, 748, 786
NlaIII CATG 2 cut(s) 263, 359
NlaIV GGNNCC 1 cut(s) 588
NmeAIII GCCGAG 1 cut(s) 923
NmuCI GTSAC 1 cut(s) 515
NsiI ATGCAT 1 cut(s) 868
PfeI GAWTC 3 cut(s) 314, 419, 928
PkrI GCNGC 7 cut(s) 27, 30, 62, 113, 116, 619, 811
PleI GAGTC 3 cut(s) 337, 512, 619
PpsI GAGTC 3 cut(s) 337, 512, 619
PsiI TTATAA 1 cut(s) 843
Psp124BI GAGCTC 1 cut(s) 837
PspFI CCCAGC 1 cut(s) 379
PspN4I GGNNCC 1 cut(s) 588
PspOMI GGGCCC 1 cut(s) 586
PspPI GGNCC 2 cut(s) 586, 587
PstI CTGCAG 1 cut(s) 619
PstNI CAGNNNCTG 1 cut(s) 308
PsuI RGATCY 2 cut(s) 305, 497
PvuII CAGCTG 1 cut(s) 1023
RsaI GTAC 2 cut(s) 457, 892
RsaNI GTAC 2 cut(s) 456, 891
SacI GAGCTC 1 cut(s) 837
SaqAI TTAA 4 cut(s) 122, 216, 624, 1043
SatI GCNGC 7 cut(s) 26, 29, 61, 112, 115, 618, 810
Sau3AI GATC 6 cut(s) 189, 305, 424, 497, 748, 786
Sau96I GGNCC 2 cut(s) 586, 587
SchI GAGTC 3 cut(s) 338, 512, 620
ScrFI CCNGG 2 cut(s) 97, 570
SduI GDGCHC 3 cut(s) 413, 590, 837
SfaNI GCATC 4 cut(s) 133, 834, 853, 875
SfcI CTRYAG 2 cut(s) 414, 615
Sse9I AATT 5 cut(s) 130, 177, 182, 621, 1040
SsiI CCGC 3 cut(s) 115, 494, 809
SspI AATATT 1 cut(s) 732
SspMI CTAG 3 cut(s) 606, 725, 1054
SstI GAGCTC 1 cut(s) 837
StyD4I CCNGG 2 cut(s) 95, 568
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 4 cut(s) 155, 491, 883, 996
TaiI ACGT 1 cut(s) 896
TaqI TCGA 2 cut(s) 79, 747
TasI AATT 5 cut(s) 130, 177, 182, 621, 1040
TauI GCSGC 2 cut(s) 117, 812
TfiI GAWTC 3 cut(s) 314, 419, 928
Tru1I TTAA 4 cut(s) 122, 216, 624, 1043
Tru9I TTAA 4 cut(s) 122, 216, 624, 1043
TscAI CASTG 6 cut(s) 370, 496, 520, 588, 789, 954
TseFI GTSAC 1 cut(s) 515
TseI GCWGC 5 cut(s) 25, 28, 60, 111, 617
Tsp45I GTSAC 1 cut(s) 515
TspDTI ATGAA 3 cut(s) 657, 866, 1004
TspRI CASTG 6 cut(s) 370, 496, 520, 588, 789, 954
XapI RAATTY 1 cut(s) 182
XspI CTAG 3 cut(s) 606, 725, 1054
Zsp2I ATGCAT 1 cut(s) 868
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.