Prupe.4G212900_v2.0.a1

Serine threonine-protein kinase PBS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0021232
Physical Location & Seq
Reverse (-)
13282614 .. 13284196
1583 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G212900.1_v2.0.a1.CDS.1

Sequence Viewer

Length: 327 bp
ATGTTGTCAGCCGGTTTCTATCAGGTTTCCTCCGCAAGTAAGCCTCTTTATGCACTAATTAGTTTGCTGTTGGCCATTGCGGCTGTGCTTACTTGCATCCTGGAGCTCATTCACAATGGTATAAAGGACAAAGATGCGTCTCCGAATAGACCTGTTTTTGGTACGTTGCCTGACATTTTCGGATTGGGACTTGCTGTGATTCAATGTGTTTGCTCGGCAGTGCAGTATTATTTCCTCCATCAGCATGCTAGTAATCCTATGAAACTGCCCCCTCTGCCTCTCTTTTTCTTCACCTATTTGGTGGTTTTCAAATTGAAAGGGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

109

Amino Acids

11.81

Weight (kDa)

8.86

Isoelectric Point (pI)

22.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 33, 80
AcoI YGGCCR 1 cut(s) 72
AfaI GTAC 1 cut(s) 163
AfiI CCNNNNNNNGG 1 cut(s) 158
AgsI TTSAA 3 cut(s) 203, 310, 316
AjnI CCWGG 1 cut(s) 99
AluBI AGCT 1 cut(s) 106
AluI AGCT 1 cut(s) 106
Alw21I GWGCWC 1 cut(s) 108
Alw26I GTCTC 1 cut(s) 144
AoxI GGCC 1 cut(s) 72
AsuHPI GGTGA 1 cut(s) 283
BalI TGGCCA 1 cut(s) 74
BanII GRGCYC 1 cut(s) 108
Bbv12I GWGCWC 1 cut(s) 108
BccI CCATC 1 cut(s) 246
BciT130I CCWGG 1 cut(s) 101
BcoDI GTCTC 1 cut(s) 144
BfaI CTAG 2 cut(s) 249, 325
BglI GCCNNNNNGGC 1 cut(s) 80
BisI GCNGC 1 cut(s) 81
BlsI GCNGC 1 cut(s) 82
Bme1390I CCNGG 1 cut(s) 101
BmrFI CCNGG 1 cut(s) 101
BmsI GCATC 2 cut(s) 105, 124
BpmI CTGGAG 1 cut(s) 122
Bsc4I CCNNNNNNNGG 1 cut(s) 158
Bse118I RCCGGY 1 cut(s) 11
Bse3DI GCAATG 1 cut(s) 75
BseBI CCWGG 1 cut(s) 101
BseGI GGATG 1 cut(s) 96
BseLI CCNNNNNNNGG 1 cut(s) 158
BseMI GCAATG 1 cut(s) 75
BsgI GTGCAG 1 cut(s) 242
BshFI GGCC 1 cut(s) 74
BsiHKAI GWGCWC 1 cut(s) 108
BsiSI CCGG 1 cut(s) 12
BslFI GGGAC 1 cut(s) 201
BslI CCNNNNNNNGG 1 cut(s) 158
BsmAI GTCTC 1 cut(s) 144
BsmBI CGTCTC 1 cut(s) 144
BsmFI GGGAC 1 cut(s) 201
BsnI GGCC 1 cut(s) 74
Bsp1286I GDGCHC 1 cut(s) 108
BspACI CCGC 2 cut(s) 33, 80
BspANI GGCC 1 cut(s) 74
BsrDI GCAATG 1 cut(s) 75
BsrFI RCCGGY 1 cut(s) 11
BssAI RCCGGY 1 cut(s) 11
Bst2UI CCWGG 1 cut(s) 101
BstC8I GCNNGC 1 cut(s) 246
BstF5I GGATG 1 cut(s) 96
BstMAI GTCTC 1 cut(s) 144
BstMWI GCNNNNNNNGC 2 cut(s) 80, 274
BstNI CCWGG 1 cut(s) 101
BstNSI RCATGY 1 cut(s) 248
BstSCI CCNGG 1 cut(s) 99
BsuRI GGCC 1 cut(s) 74
BtsCI GGATG 1 cut(s) 96
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 1 cut(s) 225
Cac8I GCNNGC 1 cut(s) 246
Cfr10I RCCGGY 1 cut(s) 11
CseI GACGC 1 cut(s) 126
Csp6I GTAC 1 cut(s) 162
CviAII CATG 1 cut(s) 245
CviJI RGCY 5 cut(s) 11, 43, 74, 83, 106
CviKI_1 RGCY 5 cut(s) 11, 43, 74, 83, 106
CviQI GTAC 1 cut(s) 162
EaeI YGGCCR 1 cut(s) 72
Ecl136II GAGCTC 1 cut(s) 106
Eco24I GRGCYC 1 cut(s) 108
Eco53kI GAGCTC 1 cut(s) 106
EcoICRI GAGCTC 1 cut(s) 106
EcoRII CCWGG 1 cut(s) 99
EcoT38I GRGCYC 1 cut(s) 108
Esp3I CGTCTC 1 cut(s) 144
FaeI CATG 1 cut(s) 248
FaiI YATR 4 cut(s) 51, 122, 246, 260
FaqI GGGAC 1 cut(s) 201
FatI CATG 1 cut(s) 244
Fnu4HI GCNGC 1 cut(s) 81
FokI GGATG 1 cut(s) 83
FriOI GRGCYC 1 cut(s) 108
Fsp4HI GCNGC 1 cut(s) 81
FspBI CTAG 2 cut(s) 249, 325
GluI GCNGC 1 cut(s) 81
GsuI CTGGAG 1 cut(s) 122
HaeIII GGCC 1 cut(s) 74
HapII CCGG 1 cut(s) 12
HgaI GACGC 1 cut(s) 126
Hin1II CATG 1 cut(s) 248
HinfI GANTC 1 cut(s) 199
HpaII CCGG 1 cut(s) 12
HphI GGTGA 1 cut(s) 283
Hpy188I TCNGA 2 cut(s) 144, 182
HpyCH4IV ACGT 1 cut(s) 164
HpyCH4V TGCA 3 cut(s) 53, 96, 223
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 274
HpySE526I ACGT 1 cut(s) 164
Hsp92II CATG 1 cut(s) 248
LmnI GCTCC 1 cut(s) 103
LpnPI CCDG 6 cut(s) 8, 25, 86, 113, 165, 183
LweI GCATC 2 cut(s) 105, 124
MaeI CTAG 2 cut(s) 249, 325
MaeII ACGT 1 cut(s) 164
MboII GAAGA 1 cut(s) 280
MhlI GDGCHC 1 cut(s) 108
MlsI TGGCCA 1 cut(s) 74
MluCI AATT 2 cut(s) 57, 311
MluNI TGGCCA 1 cut(s) 74
MnlI CCTC 5 cut(s) 40, 54, 245, 282, 288
Mox20I TGGCCA 1 cut(s) 74
MscI TGGCCA 1 cut(s) 74
MslI CAYNNNNRTG 1 cut(s) 243
Msp20I TGGCCA 1 cut(s) 74
MspI CCGG 1 cut(s) 12
MspR9I CCNGG 1 cut(s) 101
MvaI CCWGG 1 cut(s) 101
MwoI GCNNNNNNNGC 2 cut(s) 80, 274
NlaIII CATG 1 cut(s) 248
NmeAIII GCCGAG 1 cut(s) 194
NspI RCATGY 1 cut(s) 248
PaeI GCATGC 1 cut(s) 248
PfeI GAWTC 1 cut(s) 199
PfoI TCCNGGA 1 cut(s) 99
PkrI GCNGC 1 cut(s) 82
Psp124BI GAGCTC 1 cut(s) 108
Psp6I CCWGG 1 cut(s) 99
PspGI CCWGG 1 cut(s) 99
RsaI GTAC 1 cut(s) 163
RsaNI GTAC 1 cut(s) 162
RseI CAYNNNNRTG 1 cut(s) 243
SacI GAGCTC 1 cut(s) 108
SatI GCNGC 1 cut(s) 81
ScrFI CCNGG 1 cut(s) 101
SduI GDGCHC 1 cut(s) 108
SetI ASST 5 cut(s) 27, 108, 154, 167, 296
SfaNI GCATC 2 cut(s) 105, 124
SmiMI CAYNNNNRTG 1 cut(s) 243
SphI GCATGC 1 cut(s) 248
Sse9I AATT 2 cut(s) 57, 311
SsiI CCGC 2 cut(s) 33, 80
SspMI CTAG 2 cut(s) 249, 325
SstI GAGCTC 1 cut(s) 108
StyD4I CCNGG 1 cut(s) 99
TaiI ACGT 1 cut(s) 167
TasI AATT 2 cut(s) 57, 311
TauI GCSGC 1 cut(s) 83
TfiI GAWTC 1 cut(s) 199
TscAI CASTG 1 cut(s) 225
TspDTI ATGAA 1 cut(s) 275
TspRI CASTG 1 cut(s) 225
XceI RCATGY 1 cut(s) 248
XspI CTAG 2 cut(s) 249, 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.