Prupe.6G111000_v2.0.a1

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0021229
Physical Location & Seq
Forward (+)
7969786 .. 7975586
5801 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G111000.2

Sequence Viewer

Length: 1722 bp
ATGGATACCAATAATTGGAGGCCTCCTCAAGTTGGAGAGGCCCCCATGGATGCAGGCGATTGGAGGAGTCAACTGCAGCCGGATTCAAGGCAAAGAATTGTAAACAAGATAATGGATACATTGAAGAGGCATCTCCCTTTCTCTGGTCATGAGGGATTACAGGAGCTCAAGAAAATTGCTGTAAGGTTTGAGGAAAAGATTTATACTGCTGCCACAAGCCAGTCAGATTACCTACGGAAGATTAGTCTGAAGATGCTAACAATGGAGACCAAGTCTCAGAACACAATGCCCAATTCTTTAGAATCCAACTCTGCTGGTAATAGTAACAGGCCCCCTGATCCAGGGTCTTTTGGTATGCAACCCCAAGTCCCCAATCAAGGGCAATCACTCTCTGTGCCGTTGCCAGCTAATCAACCTCAGCCACATCAACAACTCTTATCACAGAACATTCAGAATAACATTCCAGCTGCTGGAGTTCAAAGTTCTGCTGGCTTATCATCTGCTCTACCTCCTTCATCCGGTTTAACCCAGACTCCTATTCCAAGTATTGTTGGGCAAAATCAAATCATGCAGAATATGGGCCAGGGGGTACCCTCCAATATGTTTGCTACTTCTCAAAGGCAATTGCCAGGAAGGCAACAGGTAGTTCCACAACAGCAGCAGCAGCAGCAGCAATCCCAGAATTCCCAGCAGTATATGTACCATCAACAGTTGCAACATCCGCTTTTGAAGCCCAAATATCAGCAGGGAAATATCCCACAACTTGTCCAACAACAGCAACAGCAGCCGAACCTATTACAACCAACTCAGTTGCAGTCTTCTCAGCAACCTGTTATGCAAACATCATCTGTTATTCAACCTGCTGTGGTGCAATCATCTCTCTCTAGTCTTCAGCTGAATCAACAATCTGCAATTCAACAGTCAACACCATCTATGCTTCAGCAGCATCCACAGTCAGTTCTCAGACAGCCTCAACAACAACGGGCCTCTGTTGTTCACCAACAGCAAACATCCCAACAGCCAATATTTCCCCCACAGCAGCAGCAACAGCAGCAGCTTATGGTGCAACAGTCAAATGCTACGAGCTTGTCGCAGAATCAGCTAATCAGACAACAAAACAATGTTGGGGATATGCAGCAGCAACAGCAACAACAGAGGTTACTGAGCCAGCAGAATAACATTTTAAACCTTCAACAACAGCAGCAGCAGTTTATGGCTCAGCAAAGCAACCTTTCAAATATGCATCAGCCACAGTTGGGCCCTCAGAGTAATGTTACTGGGTTACAGCAACAGCAGCACCTTGGAACTCAAAGTGGTAACTCAAGCATGCAAACTAATCAGCACTCTGTACACTTGTTGCAACAAACCAAGGTTCAAGGGCAGCAACAACAACATCAGAGTTCATCTAACTTGTTACCCACTCAAAGACAGCAATCACAGCCTACACAGATGCAGCAATTGGGTTTGCAACAGCAGTCAAATCCACTGCAACGAGATATGCAGCAAAGACTTCAAGCCTCAGGTCAGGTTCCTGGAACCATGCTGCAACCACAAAGTGTAATGGATCAGCAAAAGCAGTTATATCAATCTCAAAGACCCCTTCCTGAGACATCATCGACATCTCTAGATTCCACTGCCCAGACTGGACATGCAACTGGGGGTGATTGGCAAGAGGAGGTCTTTCAAAAAGTAGGATGCCCTGACTTGTTGTCCATTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

574

Amino Acids

64.15

Weight (kDa)

9.74

Isoelectric Point (pI)

84.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021229)

Species Orthologous Gene IDs
prunus_persica Prupe.6G111000_v2.0.a1 Prupe.6G111000_v2.0.a1 Prupe.6G111000_v2.0.a1 Prupe.6G111000_v2.0.a1
pyrus_communis pycom03g02620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 868
Acc65I GGTACC 1 cut(s) 589
AccB1I GGYRCC 1 cut(s) 589
AccB7I CCANNNNNTGG 2 cut(s) 15, 470
AciI CCGC 1 cut(s) 722
AclWI GGATC 2 cut(s) 332, 1572
AcsI RAATTY 1 cut(s) 682
AcuI CTGAAG 3 cut(s) 269, 875, 923
AdeI CACNNNGTG 1 cut(s) 1556
AfaI GTAC 3 cut(s) 591, 701, 1350
AfiI CCNNNNNNNGG 9 cut(s) 15, 32, 143, 341, 377, 378, 470, 518, 1256
AhdI GACNNNNNGTC 1 cut(s) 1708
AjnI CCWGG 4 cut(s) 340, 582, 628, 1531
AluBI AGCT 7 cut(s) 166, 407, 467, 895, 1057, 1086, 1102
AluI AGCT 7 cut(s) 166, 407, 467, 895, 1057, 1086, 1102
Alw21I GWGCWC 1 cut(s) 168
Alw26I GTCTC 3 cut(s) 260, 279, 1601
AlwI GGATC 2 cut(s) 332, 1572
AlwNI CAGNNNCTG 1 cut(s) 470
AoxI GGCC 6 cut(s) 20, 39, 329, 580, 984, 1258
ApaI GGGCCC 1 cut(s) 1262
ApoI RAATTY 1 cut(s) 682
Asp718I GGTACC 1 cut(s) 589
AspS9I GGNCC 6 cut(s) 40, 330, 580, 984, 1258, 1259
AsuHPI GGTGA 2 cut(s) 989, 1673
AxyI CCTNAGG 1 cut(s) 1519
BaeGI GKGCMC 1 cut(s) 1262
BanI GGYRCC 1 cut(s) 589
BanII GRGCYC 2 cut(s) 168, 1262
BbsI GAAGAC 2 cut(s) 810, 881
Bbv12I GWGCWC 1 cut(s) 168
BbvCI CCTCAGC 1 cut(s) 417
BccI CCATC 2 cut(s) 711, 937
BceAI ACGGC 1 cut(s) 382
BciT130I CCWGG 4 cut(s) 342, 584, 630, 1533
BciVI GTATCC 1 cut(s) 109
BcoDI GTCTC 3 cut(s) 260, 279, 1601
BfaI CTAG 2 cut(s) 885, 1625
BfmI CTRYAG 1 cut(s) 74
BfuAI ACCTGC 1 cut(s) 868
BfuI GTATCC 1 cut(s) 109
BglI GCCNNNNNGGC 1 cut(s) 634
BlpI GCTNAGC 1 cut(s) 1218
Bme1390I CCNGG 4 cut(s) 342, 584, 630, 1533
BmeRI GACNNNNNGTC 1 cut(s) 1708
BmgT120I GGNCC 6 cut(s) 40, 330, 580, 984, 1258, 1259
BmiI GGNNCC 6 cut(s) 42, 332, 591, 1260, 1530, 1537
BmrFI CCNGG 4 cut(s) 342, 584, 630, 1533
BmrI ACTGGG 2 cut(s) 1287, 1665
BmsI GCATC 7 cut(s) 40, 139, 243, 955, 1252, 1440, 1685
BmuI ACTGGG 2 cut(s) 1287, 1665
BpiI GAAGAC 2 cut(s) 810, 881
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpmI CTGGAG 1 cut(s) 492
Bpu10I CCTNAGC 1 cut(s) 417
Bpu1102I GCTNAGC 1 cut(s) 1218
BpuEI CTTGAG 3 cut(s) 12, 152, 1306
BsaI GGTCTC 1 cut(s) 260
BsaJI CCNNGG 5 cut(s) 45, 341, 583, 1300, 1368
BsaWI WCCGGW 1 cut(s) 518
BsaXI ACNNNNNCTCC 4 cut(s) 257, 287, 517, 547
Bsc4I CCNNNNNNNGG 9 cut(s) 15, 32, 143, 341, 377, 378, 470, 518, 1256
Bse1I ACTGG 4 cut(s) 220, 1282, 1648, 1660
Bse21I CCTNAGG 1 cut(s) 1519
BseBI CCWGG 4 cut(s) 342, 584, 630, 1533
BseDI CCNNGG 5 cut(s) 45, 341, 583, 1300, 1368
BseGI GGATG 6 cut(s) 55, 515, 718, 946, 1010, 1700
BseLI CCNNNNNNNGG 9 cut(s) 15, 32, 143, 341, 377, 378, 470, 518, 1256
BseNI ACTGG 4 cut(s) 220, 1282, 1648, 1660
BseRI GAGGAG 3 cut(s) 15, 79, 1688
BseSI GKGCMC 1 cut(s) 1262
BseYI CCCAGC 1 cut(s) 687
BshFI GGCC 6 cut(s) 22, 41, 331, 582, 986, 1260
BshNI GGYRCC 1 cut(s) 589
BsiHKAI GWGCWC 1 cut(s) 168
BsiSI CCGG 2 cut(s) 80, 519
BslFI GGGAC 1 cut(s) 353
BslI CCNNNNNNNGG 9 cut(s) 15, 32, 143, 341, 377, 378, 470, 518, 1256
BsmAI GTCTC 3 cut(s) 260, 279, 1601
BsmFI GGGAC 1 cut(s) 353
BsnI GGCC 6 cut(s) 22, 41, 331, 582, 986, 1260
Bso31I GGTCTC 1 cut(s) 260
Bsp120I GGGCCC 1 cut(s) 1258
Bsp1286I GDGCHC 2 cut(s) 168, 1262
Bsp1407I TGTACA 1 cut(s) 1348
Bsp143I GATC 2 cut(s) 337, 1564
Bsp1720I GCTNAGC 1 cut(s) 1218
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 1 cut(s) 722
BspANI GGCC 6 cut(s) 22, 41, 331, 582, 986, 1260
BspHI TCATGA 1 cut(s) 148
BspLI GGNNCC 6 cut(s) 42, 332, 591, 1260, 1530, 1537
BspMAI CTGCAG 1 cut(s) 78
BspMI ACCTGC 1 cut(s) 868
BspPI GGATC 2 cut(s) 332, 1572
BspT107I GGYRCC 1 cut(s) 589
BspTNI GGTCTC 1 cut(s) 260
BsrGI TGTACA 1 cut(s) 1348
BsrI ACTGG 4 cut(s) 220, 1282, 1648, 1660
BssECI CCNNGG 5 cut(s) 45, 341, 583, 1300, 1368
BssMI GATC 2 cut(s) 337, 1564
BssT1I CCWWGG 3 cut(s) 45, 1300, 1368
Bst2UI CCWGG 4 cut(s) 342, 584, 630, 1533
Bst4CI ACNGT 5 cut(s) 711, 921, 954, 1071, 1254
Bst6I CTCTTC 1 cut(s) 119
BstAUI TGTACA 1 cut(s) 1348
BstC8I GCNNGC 5 cut(s) 55, 405, 490, 1169, 1328
BstDSI CCRYGG 1 cut(s) 45
BstENI CCTNNNNNAGG 1 cut(s) 339
BstF5I GGATG 6 cut(s) 55, 515, 718, 946, 1010, 1700
BstKTI GATC 2 cut(s) 340, 1567
BstMAI GTCTC 3 cut(s) 260, 279, 1601
BstMBI GATC 2 cut(s) 337, 1564
BstNI CCWGG 4 cut(s) 342, 584, 630, 1533
BstNSI RCATGY 2 cut(s) 1330, 1652
BstSCI CCNGG 4 cut(s) 340, 582, 628, 1531
BstSFI CTRYAG 1 cut(s) 74
BstSLI GKGCMC 1 cut(s) 1262
BstV2I GAAGAC 2 cut(s) 810, 881
Bsu36I CCTNAGG 1 cut(s) 1519
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 6 cut(s) 22, 41, 331, 582, 986, 1260
BtgI CCRYGG 1 cut(s) 45
BtsCI GGATG 6 cut(s) 55, 515, 718, 946, 1010, 1700
BtsI GCAGTG 2 cut(s) 1484, 1632
BtsIMutI CAGTG 2 cut(s) 1484, 1632
BveI ACCTGC 1 cut(s) 868
Cac8I GCNNGC 5 cut(s) 55, 405, 490, 1169, 1328
CaiI CAGNNNCTG 1 cut(s) 470
CciI TCATGA 1 cut(s) 148
Cfr13I GGNCC 6 cut(s) 40, 330, 580, 984, 1258, 1259
Csp6I GTAC 3 cut(s) 590, 700, 1349
CviAII CATG 6 cut(s) 46, 149, 568, 1327, 1540, 1649
CviQI GTAC 3 cut(s) 590, 700, 1349
DpnI GATC 2 cut(s) 339, 1566
DpnII GATC 2 cut(s) 337, 1564
DraI TTTAAA 1 cut(s) 1185
DraIII CACNNNGTG 1 cut(s) 1556
DriI GACNNNNNGTC 1 cut(s) 1708
Eam1104I CTCTTC 1 cut(s) 119
Eam1105I GACNNNNNGTC 1 cut(s) 1708
EarI CTCTTC 1 cut(s) 119
Ecl136II GAGCTC 1 cut(s) 166
Eco130I CCWWGG 3 cut(s) 45, 1300, 1368
Eco147I AGGCCT 1 cut(s) 22
Eco24I GRGCYC 2 cut(s) 168, 1262
Eco31I GGTCTC 1 cut(s) 260
Eco53kI GAGCTC 1 cut(s) 166
Eco57I CTGAAG 3 cut(s) 269, 875, 923
Eco81I CCTNAGG 1 cut(s) 1519
EcoICRI GAGCTC 1 cut(s) 166
EcoNI CCTNNNNNAGG 1 cut(s) 339
EcoO109I RGGNCCY 3 cut(s) 40, 330, 1259
EcoRI GAATTC 1 cut(s) 682
EcoRII CCWGG 4 cut(s) 340, 582, 628, 1531
EcoT14I CCWWGG 3 cut(s) 45, 1300, 1368
EcoT22I ATGCAT 1 cut(s) 1245
EcoT38I GRGCYC 2 cut(s) 168, 1262
ErhI CCWWGG 3 cut(s) 45, 1300, 1368
FaeI CATG 6 cut(s) 49, 152, 571, 1330, 1543, 1652
FaqI GGGAC 1 cut(s) 353
FatI CATG 6 cut(s) 45, 148, 567, 1326, 1539, 1648
FokI GGATG 6 cut(s) 62, 502, 705, 933, 997, 1707
FriOI GRGCYC 2 cut(s) 168, 1262
FspBI CTAG 2 cut(s) 885, 1625
GsaI CCCAGC 1 cut(s) 691
GsuI CTGGAG 1 cut(s) 492
HaeIII GGCC 6 cut(s) 22, 41, 331, 582, 986, 1260
HapII CCGG 2 cut(s) 80, 519
Hin1II CATG 6 cut(s) 49, 152, 571, 1330, 1543, 1652
HincII GTYRAC 2 cut(s) 71, 924
HindII GTYRAC 2 cut(s) 71, 924
HinfI GANTC 7 cut(s) 67, 83, 302, 532, 898, 1096, 1628
HpaII CCGG 2 cut(s) 80, 519
HphI GGTGA 2 cut(s) 989, 1673
Hpy166II GTNNAC 5 cut(s) 71, 103, 924, 997, 1351
Hpy188I TCNGA 8 cut(s) 226, 249, 279, 453, 965, 1109, 1266, 1398
Hpy188III TCNNGA 4 cut(s) 149, 169, 1604, 1625
Hpy8I GTNNAC 5 cut(s) 71, 103, 924, 997, 1351
HpyAV CCTTC 4 cut(s) 522, 627, 1199, 1610
HpyCH4III ACNGT 5 cut(s) 711, 921, 954, 1071, 1254
Hsp92II CATG 6 cut(s) 49, 152, 571, 1330, 1543, 1652
KpnI GGTACC 1 cut(s) 593
Kzo9I GATC 2 cut(s) 337, 1564
LmnI GCTCC 1 cut(s) 163
LweI GCATC 7 cut(s) 40, 139, 243, 955, 1252, 1440, 1685
MaeI CTAG 2 cut(s) 885, 1625
MaeIII GTNAC 6 cut(s) 323, 1158, 1273, 1281, 1316, 1413
MalI GATC 2 cut(s) 339, 1566
MboI GATC 2 cut(s) 337, 1564
MboII GAAGA 5 cut(s) 136, 250, 262, 810, 881
MfeI CAATTG 2 cut(s) 623, 1457
MhlI GDGCHC 2 cut(s) 168, 1262
MluCI AATT 8 cut(s) 13, 96, 174, 292, 623, 682, 912, 1457
MlyI GAGTC 2 cut(s) 76, 526
MmeI TCCRAC 3 cut(s) 13, 330, 793
Mph1103I ATGCAT 1 cut(s) 1245
MseI TTAA 3 cut(s) 524, 1184, 1716
MspA1I CMGCKG 2 cut(s) 467, 895
MspI CCGG 2 cut(s) 80, 519
MspR9I CCNGG 4 cut(s) 342, 584, 630, 1533
MunI CAATTG 2 cut(s) 623, 1457
MvaI CCWGG 4 cut(s) 342, 584, 630, 1533
NcoI CCATGG 1 cut(s) 45
NdeII GATC 2 cut(s) 337, 1564
NlaIII CATG 6 cut(s) 49, 152, 571, 1330, 1543, 1652
NlaIV GGNNCC 6 cut(s) 42, 332, 591, 1260, 1530, 1537
NsiI ATGCAT 1 cut(s) 1245
NspI RCATGY 2 cut(s) 1330, 1652
PaeI GCATGC 1 cut(s) 1330
PagI TCATGA 1 cut(s) 148
PceI AGGCCT 1 cut(s) 22
PfeI GAWTC 5 cut(s) 83, 302, 898, 1096, 1628
PflFI GACNNNGTC 1 cut(s) 271
PflMI CCANNNNNTGG 2 cut(s) 15, 470
PfoI TCCNGGA 1 cut(s) 1531
PleI GAGTC 2 cut(s) 75, 526
PpsI GAGTC 2 cut(s) 75, 526
Psp124BI GAGCTC 1 cut(s) 168
Psp6I CCWGG 4 cut(s) 340, 582, 628, 1531
PspFI CCCAGC 1 cut(s) 687
PspGI CCWGG 4 cut(s) 340, 582, 628, 1531
PspN4I GGNNCC 6 cut(s) 42, 332, 591, 1260, 1530, 1537
PspOMI GGGCCC 1 cut(s) 1258
PspPI GGNCC 6 cut(s) 40, 330, 580, 984, 1258, 1259
PstI CTGCAG 1 cut(s) 78
PstNI CAGNNNCTG 1 cut(s) 470
PsyI GACNNNGTC 1 cut(s) 271
PvuII CAGCTG 2 cut(s) 467, 895
RsaI GTAC 3 cut(s) 591, 701, 1350
RsaNI GTAC 3 cut(s) 590, 700, 1349
SacI GAGCTC 1 cut(s) 168
SaqAI TTAA 3 cut(s) 524, 1184, 1716
Sau3AI GATC 2 cut(s) 337, 1564
Sau96I GGNCC 6 cut(s) 40, 330, 580, 984, 1258, 1259
SchI GAGTC 2 cut(s) 76, 526
ScrFI CCNGG 4 cut(s) 342, 584, 630, 1533
SduI GDGCHC 2 cut(s) 168, 1262
SfaNI GCATC 7 cut(s) 40, 139, 243, 955, 1252, 1440, 1685
SfcI CTRYAG 1 cut(s) 74
SmlI CTYRAG 3 cut(s) 27, 167, 1321
SmoI CTYRAG 3 cut(s) 27, 167, 1321
SphI GCATGC 1 cut(s) 1330
Sse9I AATT 8 cut(s) 13, 96, 174, 292, 623, 682, 912, 1457
SseBI AGGCCT 1 cut(s) 22
SsiI CCGC 1 cut(s) 722
SspI AATATT 1 cut(s) 1026
SspMI CTAG 2 cut(s) 885, 1625
SstI GAGCTC 1 cut(s) 168
StuI AGGCCT 1 cut(s) 22
StyD4I CCNGG 4 cut(s) 340, 582, 628, 1531
StyI CCWWGG 3 cut(s) 45, 1300, 1368
TaaI ACNGT 5 cut(s) 711, 921, 954, 1071, 1254
TaqI TCGA 1 cut(s) 1616
TasI AATT 8 cut(s) 13, 96, 174, 292, 623, 682, 912, 1457
TatI WGTACW 1 cut(s) 1348
TfiI GAWTC 5 cut(s) 83, 302, 898, 1096, 1628
Tru1I TTAA 3 cut(s) 524, 1184, 1716
Tru9I TTAA 3 cut(s) 524, 1184, 1716
TscAI CASTG 2 cut(s) 1491, 1639
TspDTI ATGAA 2 cut(s) 504, 1392
TspGWI ACGGA 1 cut(s) 250
TspRI CASTG 2 cut(s) 1491, 1639
Tth111I GACNNNGTC 1 cut(s) 271
Van91I CCANNNNNTGG 2 cut(s) 15, 470
XagI CCTNNNNNAGG 1 cut(s) 339
XapI RAATTY 1 cut(s) 682
XbaI TCTAGA 1 cut(s) 1624
XceI RCATGY 2 cut(s) 1330, 1652
XspI CTAG 2 cut(s) 885, 1625
Zsp2I ATGCAT 1 cut(s) 1245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.