Prupe.7G108200_v2.0.a1
HSP70 Family

Heat shock cognate 70 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0018916
Physical Location & Seq
Forward (+)
13672249 .. 13675173
2925 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G108200.1

Sequence Viewer

Length: 2250 bp
ATGGCCGTAGAGAACATGCCACCATTTCCAGTTCCAATCAAACTTACGAGTGATATATATAACTATCAACAATGGAAATATATTTCTCTTTCATATTTTGACTGCCACAATCTTTCTGGAATCATTCATGGAACAGAGCCTCGTCCTCGGGGACTTTGGTCTACCTTTAGTAATTGGCCCGGCAGACATCAAAAGGGTTCGAGTTGGTTCAACAGAGAGCAAAAGGCTATGAATTGGCTAAAAGCCACTCTATCTGAAAGTCTCCAACAAATTGTCATGGCCGGAGCTGACTCTTCACGAAATGTCTGGCTAAATCTCGAGGAACACTTCGCCCATCTCTCTCATGCTCGCATTTATCAGCTTAAATCTGATCTACACAAAGTCAAGAAAGATCCCACTATTCCGATGGCTGAGTATCTGGAAAAAATCAAACAGCTGGCGACCGATCTCGCAGCCGCCAGTGCTCCTGTGGAAAGTCAAGACCTACTCCATGTACACATCCTGGCGGGATTACCAGAGCAGTACAATCCCGTTGTTAAATGGATAAAGCACAATCCAGTAAGTGGTTGGGATGACTTGTGCGAGTTATTGTTGAAAGAGGAAATGCGTCTGGATCCACAGCGCACATTACGACTTAGACACACTACTCCTCCCTCTCCTCCTCAAGAAGAAGAGTACGCAATTGGGATCGATCTGGGAACGACCTATTCCCGCGTAGCCGTGTGGCAGAAGGATCATGTAGAAATCATCCACAATGATCACGGGAACAGGAAGACTGCATCTTATGTTGCCTTCACTGAAACTGATGAGACTCATTTGGTAGGTGATGCAGCATTTAACCAAGTCGTCAGAAACACAGCCAACTCCATCTTTGATACAAAGAGGTTAATCGGTAGGAGATTCAGTGACCCCTCTGTTCAAAGTGATGTGAAGCTCTGGCCATTCAAGGTCATTGAAGGTCCAGGTGACAAGCCCATGATTGTGGTTGCACACAATGGCCAAGAGAAACAATGTTCTGCTGAAGATATCTCATCCATGGTTCTGGAAAAGATGAGGAAGATTTCCGAGACCTACTTGGGCTCAACTGTGAAAAATGCAGTTATCACTGTCCCTGCTTACTTCAATTACTCACAGCGCCGAGCTACAAAAGAAGCAGGCATCTCTGCTGGCCTAAATGTATTGCATATTACGAACGAACCAAGTGCTGCTGCCATTGCTTATGGCCTTAACAAGAAGGCCGGTTGGAGTAGCCCAAAAAACGTGATGATATTTGATTTGGGTGGTGGCACTTTAGATGTGTCATTGCTTACCATGAGCTCTTCTGGTGACTTTCAAGTCAAGGCAACGGCTGGAGACACTCACCTTGGTGGCCAAGACTTCGATAGCAGATTGGTGAAATACTGTGTTGAGGAATTCAAGAGGAAGTACAAATTGGATGTTAGTGGAAATGAAAGAGCTCTTAGGAGGTTAAAAAATGAATGTGAGAAAGCGAAGAAGAGACTCTCATTTGAGTCTGACTTCGAGGTTGAAATTGACTGTTTGTGTGAGAATACTGATTTCACCATAACTTTTACTCGTGCCATATTTGAACAACTAAACGGGGATTTATTTATCAAGTGTATGGAGCCTGTGAAGAAGTGCTTAACGGATGCTAACATGGACATAAGCAGTGTCGACGATGTTGTTCTGGCCGGTGGCTCTACTAGAATTCCCATGGTGCAACAGCTATTACAGGAGTTTTTCAAGGGGAAGGAGCTGTGCAAGGGCGTTAATCCGGATGAGGCAGTGGCATATGGTGCCGCGGTTCAAGCCGCAACCTTGACTAGGAATGGCAAAGGGGAGTTTATTCAGGACTACACTCTCAAGGACGTCACTCCTCTTACAATTGGTTTGGAGTTTTCAGATAATAAGAAGTTCGTAAAGTTGATTCCAAGAAACTCACTAATTCCCGTCAAGAAGAAAATAAAATGTCCTATCAAGGATAACCCAGTTTCGGTCGACTTCCGCATGTACGAGGGTGAGAGTAGCACGCCAGCCAATCTCAACTTTTTGGGTGAATGCAGCCTCCGTTACATTCCTCCAGCTCCCAAACTTGTTCATAATTTCGATGTTTTCTTCGAAATTGATCCCGATGGCATCTTGAGTGTCTTCACTGAAGATAAGTCCTCAGGCCAGAAGAATGAGATCATAATCAACCGTGACGGACCGAAGAACTTTGAGGGAATTGAGAGAGAGGTTATGTGGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

750

Amino Acids

84.34

Weight (kDa)

6.29

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1334
AatII GACGTC 1 cut(s) 1872
AccB1I GGYRCC 1 cut(s) 1796
AccB7I CCANNNNNTGG 1 cut(s) 563
AccI GTMKAC 3 cut(s) 161, 1674, 1998
AccII CGCG 2 cut(s) 714, 1802
AccIII TCCGGA 1 cut(s) 1774
AciI CCGC 7 cut(s) 456, 506, 712, 1800, 1802, 1812, 2005
AclWI GGATC 6 cut(s) 386, 608, 621, 695, 741, 2120
AcoI YGGCCR 6 cut(s) 3, 279, 938, 997, 1369, 1689
AcsI RAATTY 2 cut(s) 1412, 1707
AcuI CTGAAG 2 cut(s) 1041, 2175
AcyI GRCGYC 1 cut(s) 1869
AfaI GTAC 5 cut(s) 495, 524, 677, 1427, 2012
AfiI CCNNNNNNNGG 3 cut(s) 563, 1824, 1993
AjnI CCWGG 2 cut(s) 501, 961
AjuI GAANNNNNNNTTGG 2 cut(s) 1415, 1447
AleI CACNNNNGTG 1 cut(s) 1365
Alw21I GWGCWC 3 cut(s) 466, 1319, 1459
Alw26I GTCTC 5 cut(s) 266, 803, 1061, 1347, 1492
AlwI GGATC 6 cut(s) 386, 608, 621, 695, 741, 2120
Ama87I CYCGRG 2 cut(s) 147, 317
Aor13HI TCCGGA 1 cut(s) 1774
ApeKI GCWGC 5 cut(s) 452, 830, 1205, 1208, 2061
ApoI RAATTY 2 cut(s) 1412, 1707
AspLEI GCGC 2 cut(s) 624, 1137
AspS9I GGNCC 3 cut(s) 177, 959, 2204
AsuC2I CCSGG 1 cut(s) 180
AsuHPI GGTGA 8 cut(s) 836, 977, 1337, 1352, 1405, 1552, 2030, 2066
AsuII TTCGAA 1 cut(s) 2118
AvaI CYCGRG 2 cut(s) 147, 317
AvaII GGWCC 2 cut(s) 959, 2204
AxyI CCTNAGG 1 cut(s) 2167
BalI TGGCCA 3 cut(s) 940, 999, 1371
BamHI GGATCC 1 cut(s) 613
BanI GGYRCC 1 cut(s) 1796
BanII GRGCYC 3 cut(s) 1082, 1319, 1459
BauI CACGAG 1 cut(s) 1575
BbsI GAAGAC 2 cut(s) 779, 2140
Bbv12I GWGCWC 3 cut(s) 466, 1319, 1459
BbvI GCAGC 5 cut(s) 464, 842, 1192, 1195, 2073
BccI CCATC 4 cut(s) 342, 400, 875, 2126
BceAI ACGGC 2 cut(s) 704, 1362
BcgI CGANNNNNNTGC 2 cut(s) 1184, 1218
BciT130I CCWGG 2 cut(s) 503, 963
BclI TGATCA 1 cut(s) 757
BcnI CCSGG 1 cut(s) 180
BcoDI GTCTC 5 cut(s) 266, 803, 1061, 1347, 1492
BfaI CTAG 2 cut(s) 1704, 1824
BfoI RGCGCY 1 cut(s) 1138
BisI GCNGC 8 cut(s) 453, 456, 831, 1206, 1209, 1800, 1812, 2062
BlsI GCNGC 8 cut(s) 454, 457, 832, 1207, 1210, 1801, 1813, 2063
Bme1390I CCNGG 3 cut(s) 180, 503, 963
Bme18I GGWCC 2 cut(s) 959, 2204
BmeT110I CYCGRG 2 cut(s) 147, 317
BmgT120I GGNCC 3 cut(s) 177, 959, 2204
BmiI GGNNCC 3 cut(s) 615, 1626, 1798
BmrFI CCNGG 3 cut(s) 180, 503, 963
BmrI ACTGGG 1 cut(s) 1982
BmsI GCATC 5 cut(s) 788, 817, 1167, 1639, 2145
BmuI ACTGGG 1 cut(s) 1982
BoxI GACNNNNGTC 1 cut(s) 157
BpiI GAAGAC 2 cut(s) 779, 2140
BpmI CTGGAG 2 cut(s) 1371, 2064
Bpu14I TTCGAA 1 cut(s) 2118
BpuEI CTTGAG 3 cut(s) 648, 1847, 2161
BpuMI CCSGG 1 cut(s) 180
Bsa29I ATCGAT 1 cut(s) 690
BsaBI GATNNNNATC 1 cut(s) 2189
BsaHI GRCGYC 1 cut(s) 1869
BsaI GGTCTC 1 cut(s) 1061
BsaJI CCNNGG 5 cut(s) 146, 1035, 1363, 1713, 1800
BsaWI WCCGGW 1 cut(s) 1774
BsaXI ACNNNNNCTCC 4 cut(s) 634, 664, 889, 919
Bsc4I CCNNNNNNNGG 3 cut(s) 563, 1824, 1993
Bse118I RCCGGY 2 cut(s) 1238, 1691
Bse1I ACTGG 4 cut(s) 29, 459, 557, 1988
Bse21I CCTNAGG 1 cut(s) 2167
Bse3DI GCAATG 2 cut(s) 1212, 1301
Bse8I GATNNNNATC 1 cut(s) 2189
BseAI TCCGGA 1 cut(s) 1774
BseBI CCWGG 2 cut(s) 503, 963
BseCI ATCGAT 1 cut(s) 690
BseDI CCNNGG 5 cut(s) 146, 1035, 1363, 1713, 1800
BseGI GGATG 7 cut(s) 498, 577, 747, 1031, 1441, 1654, 1783
BseJI GATNNNNATC 1 cut(s) 2189
BseLI CCNNNNNNNGG 3 cut(s) 563, 1824, 1993
BseMI GCAATG 2 cut(s) 1212, 1301
BseMII CTCAG 2 cut(s) 402, 2181
BseNI ACTGG 4 cut(s) 29, 459, 557, 1988
BseRI GAGGAG 4 cut(s) 639, 648, 651, 1866
BseXI GCAGC 5 cut(s) 464, 842, 1192, 1195, 2073
Bsh1236I CGCG 2 cut(s) 714, 1802
Bsh1285I CGRYCG 2 cut(s) 444, 1998
BshNI GGYRCC 1 cut(s) 1796
BshVI ATCGAT 1 cut(s) 690
BsiEI CGRYCG 2 cut(s) 444, 1998
BsiHKAI GWGCWC 3 cut(s) 466, 1319, 1459
BsiHKCI CYCGRG 2 cut(s) 147, 317
BsiSI CCGG 5 cut(s) 180, 282, 1239, 1692, 1775
BslFI GGGAC 2 cut(s) 165, 1094
BslI CCNNNNNNNGG 3 cut(s) 563, 1824, 1993
BsmAI GTCTC 5 cut(s) 266, 803, 1061, 1347, 1492
BsmFI GGGAC 2 cut(s) 165, 1094
BsmI GAATGC 1 cut(s) 2063
Bso31I GGTCTC 1 cut(s) 1061
BsoBI CYCGRG 2 cut(s) 147, 317
Bsp119I TTCGAA 1 cut(s) 2118
Bsp1286I GDGCHC 4 cut(s) 466, 1082, 1319, 1459
Bsp13I TCCGGA 1 cut(s) 1774
Bsp1407I TGTACA 1 cut(s) 493
Bsp19I CCATGG 2 cut(s) 1035, 1713
BspACI CCGC 7 cut(s) 456, 506, 712, 1800, 1802, 1812, 2005
BspCNI CTCAG 2 cut(s) 403, 2180
BspDI ATCGAT 1 cut(s) 690
BspEI TCCGGA 1 cut(s) 1774
BspFNI CGCG 2 cut(s) 714, 1802
BspLI GGNNCC 3 cut(s) 615, 1626, 1798
BspPI GGATC 6 cut(s) 386, 608, 621, 695, 741, 2120
BspQI GCTCTTC 1 cut(s) 1324
BspT104I TTCGAA 1 cut(s) 2118
BspT107I GGYRCC 1 cut(s) 1796
BspTNI GGTCTC 1 cut(s) 1061
BsrDI GCAATG 2 cut(s) 1212, 1301
BsrFI RCCGGY 2 cut(s) 1238, 1691
BsrGI TGTACA 1 cut(s) 493
BsrI ACTGG 4 cut(s) 29, 459, 557, 1988
BssAI RCCGGY 2 cut(s) 1238, 1691
BssECI CCNNGG 5 cut(s) 146, 1035, 1363, 1713, 1800
BssNI GRCGYC 1 cut(s) 1869
BssSI CACGAG 1 cut(s) 1575
BssT1I CCWWGG 3 cut(s) 1035, 1363, 1713
Bst2BI CACGAG 1 cut(s) 1575
Bst2UI CCWGG 2 cut(s) 503, 963
Bst4CI ACNGT 5 cut(s) 1087, 1108, 1403, 1538, 2198
Bst6I CTCTTC 4 cut(s) 298, 666, 1324, 1490
BstACI GRCGYC 1 cut(s) 1869
BstAPI GCANNNNNTGC 1 cut(s) 1796
BstAUI TGTACA 1 cut(s) 493
BstBI TTCGAA 1 cut(s) 2118
BstC8I GCNNGC 6 cut(s) 349, 438, 1156, 1168, 2030, 2034
BstDEI CTNAG 4 cut(s) 411, 635, 1460, 2167
BstDSI CCRYGG 3 cut(s) 1035, 1713, 1800
BstENI CCTNNNNNAGG 1 cut(s) 1822
BstF5I GGATG 7 cut(s) 498, 577, 747, 1031, 1441, 1654, 1783
BstFNI CGCG 2 cut(s) 714, 1802
BstH2I RGCGCY 1 cut(s) 1138
BstHHI GCGC 2 cut(s) 624, 1137
BstMAI GTCTC 5 cut(s) 266, 803, 1061, 1347, 1492
BstMCI CGRYCG 2 cut(s) 444, 1998
BstMWI GCNNNNNNNGC 4 cut(s) 461, 1214, 1796, 1808
BstNI CCWGG 2 cut(s) 503, 963
BstNSI RCATGY 2 cut(s) 19, 2011
BstPAI GACNNNNGTC 1 cut(s) 157
BstSCI CCNGG 3 cut(s) 178, 501, 961
BstUI CGCG 2 cut(s) 714, 1802
BstV1I GCAGC 5 cut(s) 464, 842, 1192, 1195, 2073
BstV2I GAAGAC 2 cut(s) 779, 2140
BstX2I RGATCY 2 cut(s) 391, 613
BstXI CCANNNNNNTGG 2 cut(s) 982, 1042
BstYI RGATCY 2 cut(s) 391, 613
Bsu15I ATCGAT 1 cut(s) 690
Bsu36I CCTNAGG 1 cut(s) 2167
BsuTUI ATCGAT 1 cut(s) 690
BtgI CCRYGG 3 cut(s) 1035, 1713, 1800
BtsCI GGATG 7 cut(s) 498, 577, 747, 1031, 1441, 1654, 1783
BtsI GCAGTG 2 cut(s) 1675, 1791
BtsIMutI CAGTG 7 cut(s) 466, 795, 910, 1104, 1675, 1791, 2151
Cac8I GCNNGC 6 cut(s) 349, 438, 1156, 1168, 2030, 2034
CfoI GCGC 2 cut(s) 624, 1137
Cfr10I RCCGGY 2 cut(s) 1238, 1691
Cfr13I GGNCC 3 cut(s) 177, 959, 2204
Cfr42I CCGCGG 1 cut(s) 1803
ClaI ATCGAT 1 cut(s) 690
CpoI CGGWCCG 1 cut(s) 2204
CseI GACGC 1 cut(s) 596
Csp6I GTAC 5 cut(s) 494, 523, 676, 1426, 2011
CspI CGGWCCG 1 cut(s) 2204
CviQI GTAC 5 cut(s) 494, 523, 676, 1426, 2011
DdeI CTNAG 4 cut(s) 411, 635, 1460, 2167
DrdI GACNNNNNNGTC 1 cut(s) 1334
DseDI GACNNNNNNGTC 1 cut(s) 1334
EaeI YGGCCR 6 cut(s) 3, 279, 938, 997, 1369, 1689
Eam1104I CTCTTC 4 cut(s) 298, 666, 1324, 1490
EarI CTCTTC 4 cut(s) 298, 666, 1324, 1490
Ecl136II GAGCTC 2 cut(s) 1317, 1457
Eco130I CCWWGG 3 cut(s) 1035, 1363, 1713
Eco24I GRGCYC 3 cut(s) 1082, 1319, 1459
Eco31I GGTCTC 1 cut(s) 1061
Eco32I GATATC 1 cut(s) 1027
Eco47I GGWCC 2 cut(s) 959, 2204
Eco53kI GAGCTC 2 cut(s) 1317, 1457
Eco57I CTGAAG 2 cut(s) 1041, 2175
Eco81I CCTNAGG 1 cut(s) 2167
Eco88I CYCGRG 2 cut(s) 147, 317
EcoICRI GAGCTC 2 cut(s) 1317, 1457
EcoNI CCTNNNNNAGG 1 cut(s) 1822
EcoRI GAATTC 2 cut(s) 1412, 1707
EcoRII CCWGG 2 cut(s) 501, 961
EcoRV GATATC 1 cut(s) 1027
EcoT14I CCWWGG 3 cut(s) 1035, 1363, 1713
EcoT38I GRGCYC 3 cut(s) 1082, 1319, 1459
ErhI CCWWGG 3 cut(s) 1035, 1363, 1713
FalI AAGNNNNNCTT 2 cut(s) 1625, 1657
FaqI GGGAC 2 cut(s) 165, 1094
FauI CCCGC 2 cut(s) 499, 719
FauNDI CATATG 1 cut(s) 1792
FbaI TGATCA 1 cut(s) 757
FblI GTMKAC 3 cut(s) 161, 1674, 1998
Fnu4HI GCNGC 8 cut(s) 453, 456, 831, 1206, 1209, 1800, 1812, 2062
FokI GGATG 7 cut(s) 485, 584, 734, 1018, 1448, 1661, 1790
FriOI GRGCYC 3 cut(s) 1082, 1319, 1459
Fsp4HI GCNGC 8 cut(s) 453, 456, 831, 1206, 1209, 1800, 1812, 2062
FspBI CTAG 2 cut(s) 1704, 1824
GlaI GCGC 2 cut(s) 623, 1136
GluI GCNGC 8 cut(s) 453, 456, 831, 1206, 1209, 1800, 1812, 2062
GsuI CTGGAG 2 cut(s) 1371, 2064
HaeII RGCGCY 1 cut(s) 1138
HapII CCGG 5 cut(s) 180, 282, 1239, 1692, 1775
HgaI GACGC 1 cut(s) 596
HhaI GCGC 2 cut(s) 624, 1137
Hin1I GRCGYC 1 cut(s) 1869
Hin6I GCGC 2 cut(s) 622, 1135
HinP1I GCGC 2 cut(s) 622, 1135
HincII GTYRAC 2 cut(s) 1675, 1999
HindII GTYRAC 2 cut(s) 1675, 1999
HinfI GANTC 7 cut(s) 120, 290, 811, 900, 1500, 1511, 1927
HpaII CCGG 5 cut(s) 180, 282, 1239, 1692, 1775
HphI GGTGA 8 cut(s) 836, 977, 1337, 1352, 1405, 1552, 2030, 2066
Hpy166II GTNNAC 4 cut(s) 162, 496, 1675, 1999
Hpy188I TCNGA 7 cut(s) 256, 370, 405, 851, 1066, 1516, 1903
Hpy8I GTNNAC 4 cut(s) 162, 496, 1675, 1999
Hpy99I CGWCG 1 cut(s) 1679
HpyAV CCTTC 5 cut(s) 724, 802, 950, 1228, 1744
HpyCH4III ACNGT 5 cut(s) 1087, 1108, 1403, 1538, 2198
HpyCH4IV ACGT 2 cut(s) 1260, 1869
HpyCH4V TGCA 8 cut(s) 779, 830, 989, 1097, 1183, 1720, 1761, 2061
HpyF10VI GCNNNNNNNGC 4 cut(s) 461, 1214, 1796, 1808
HpyF3I CTNAG 4 cut(s) 411, 635, 1460, 2167
HpySE526I ACGT 2 cut(s) 1260, 1869
Hsp92I GRCGYC 1 cut(s) 1869
HspAI GCGC 2 cut(s) 622, 1135
Kpn2I TCCGGA 1 cut(s) 1774
Ksp22I TGATCA 1 cut(s) 757
KspI CCGCGG 1 cut(s) 1803
LguI GCTCTTC 1 cut(s) 1324
LmnI GCTCC 5 cut(s) 284, 469, 1624, 1753, 2089
Lsp1109I GCAGC 5 cut(s) 464, 842, 1192, 1195, 2073
LweI GCATC 5 cut(s) 788, 817, 1167, 1639, 2145
MaeI CTAG 2 cut(s) 1704, 1824
MaeII ACGT 2 cut(s) 1260, 1869
MaeIII GTNAC 6 cut(s) 905, 965, 1325, 1870, 2069, 2198
MfeI CAATTG 2 cut(s) 681, 1884
MflI RGATCY 2 cut(s) 391, 613
MhlI GDGCHC 4 cut(s) 466, 1082, 1319, 1459
MlsI TGGCCA 3 cut(s) 940, 999, 1371
MluNI TGGCCA 3 cut(s) 940, 999, 1371
MlyI GAGTC 4 cut(s) 284, 805, 1494, 1520
MmeI TCCRAC 2 cut(s) 289, 1223
Mox20I TGGCCA 3 cut(s) 940, 999, 1371
MroI TCCGGA 1 cut(s) 1774
MscI TGGCCA 3 cut(s) 940, 999, 1371
MseI TTAA 9 cut(s) 363, 537, 837, 887, 1227, 1469, 1643, 1770, 2248
MslI CAYNNNNRTG 2 cut(s) 980, 1365
Msp20I TGGCCA 3 cut(s) 940, 999, 1371
MspA1I CMGCKG 2 cut(s) 436, 1802
MspI CCGG 5 cut(s) 180, 282, 1239, 1692, 1775
MspR9I CCNGG 3 cut(s) 180, 503, 963
MunI CAATTG 2 cut(s) 681, 1884
Mva1269I GAATGC 1 cut(s) 2063
MvaI CCWGG 2 cut(s) 503, 963
MvnI CGCG 2 cut(s) 714, 1802
MwoI GCNNNNNNNGC 4 cut(s) 461, 1214, 1796, 1808
NciI CCSGG 1 cut(s) 180
NcoI CCATGG 2 cut(s) 1035, 1713
NdeI CATATG 1 cut(s) 1792
NlaIV GGNNCC 3 cut(s) 615, 1626, 1798
NmeAIII GCCGAG 1 cut(s) 1163
NmuCI GTSAC 5 cut(s) 905, 965, 1325, 1870, 2198
NspI RCATGY 2 cut(s) 19, 2011
NspV TTCGAA 1 cut(s) 2118
OliI CACNNNNGTG 1 cut(s) 1365
PaeR7I CTCGAG 1 cut(s) 317
PciSI GCTCTTC 1 cut(s) 1324
PctI GAATGC 1 cut(s) 2063
PfeI GAWTC 3 cut(s) 120, 900, 1927
PflMI CCANNNNNTGG 1 cut(s) 563
PkrI GCNGC 8 cut(s) 454, 457, 832, 1207, 1210, 1801, 1813, 2063
PleI GAGTC 4 cut(s) 284, 805, 1494, 1519
PpsI GAGTC 4 cut(s) 284, 805, 1494, 1519
PshAI GACNNNNGTC 1 cut(s) 157
Psp124BI GAGCTC 2 cut(s) 1319, 1459
Psp6I CCWGG 2 cut(s) 501, 961
PspGI CCWGG 2 cut(s) 501, 961
PspN4I GGNNCC 3 cut(s) 615, 1626, 1798
PspPI GGNCC 3 cut(s) 177, 959, 2204
PsuI RGATCY 2 cut(s) 391, 613
PvuII CAGCTG 1 cut(s) 436
RsaI GTAC 5 cut(s) 495, 524, 677, 1427, 2012
RsaNI GTAC 5 cut(s) 494, 523, 676, 1426, 2011
RseI CAYNNNNRTG 2 cut(s) 980, 1365
Rsr2I CGGWCCG 1 cut(s) 2204
RsrII CGGWCCG 1 cut(s) 2204
SacI GAGCTC 2 cut(s) 1319, 1459
SacII CCGCGG 1 cut(s) 1803
SalI GTCGAC 2 cut(s) 1673, 1997
SapI GCTCTTC 1 cut(s) 1324
SaqAI TTAA 9 cut(s) 363, 537, 837, 887, 1227, 1469, 1643, 1770, 2248
SatI GCNGC 8 cut(s) 453, 456, 831, 1206, 1209, 1800, 1812, 2062
Sau96I GGNCC 3 cut(s) 177, 959, 2204
SchI GAGTC 4 cut(s) 284, 805, 1494, 1520
ScrFI CCNGG 3 cut(s) 180, 503, 963
SduI GDGCHC 4 cut(s) 466, 1082, 1319, 1459
SfaNI GCATC 5 cut(s) 788, 817, 1167, 1639, 2145
Sfr274I CTCGAG 1 cut(s) 317
Sfr303I CCGCGG 1 cut(s) 1803
SfuI TTCGAA 1 cut(s) 2118
SgrBI CCGCGG 1 cut(s) 1803
SinI GGWCC 2 cut(s) 959, 2204
SlaI CTCGAG 1 cut(s) 317
SmiMI CAYNNNNRTG 2 cut(s) 980, 1365
SmlI CTYRAG 4 cut(s) 317, 663, 1862, 2140
SmoI CTYRAG 4 cut(s) 317, 663, 1862, 2140
SsiI CCGC 7 cut(s) 456, 506, 712, 1800, 1802, 1812, 2005
SspMI CTAG 2 cut(s) 1704, 1824
SstI GAGCTC 2 cut(s) 1319, 1459
StyD4I CCNGG 3 cut(s) 178, 501, 961
StyI CCWWGG 3 cut(s) 1035, 1363, 1713
TaaI ACNGT 5 cut(s) 1087, 1108, 1403, 1538, 2198
TaiI ACGT 2 cut(s) 1263, 1872
TaqI TCGA 9 cut(s) 200, 318, 690, 1380, 1521, 1674, 1998, 2106, 2118
TaqII GACCGA 3 cut(s) 458, 1984, 2221
TatI WGTACW 3 cut(s) 493, 522, 1425
TauI GCSGC 3 cut(s) 458, 1802, 1814
TfiI GAWTC 3 cut(s) 120, 900, 1927
Tru1I TTAA 9 cut(s) 363, 537, 837, 887, 1227, 1469, 1643, 1770, 2248
Tru9I TTAA 9 cut(s) 363, 537, 837, 887, 1227, 1469, 1643, 1770, 2248
TscAI CASTG 7 cut(s) 466, 802, 910, 1111, 1675, 1791, 2158
TseFI GTSAC 5 cut(s) 905, 965, 1325, 1870, 2198
TseI GCWGC 5 cut(s) 452, 830, 1205, 1208, 2061
Tsp45I GTSAC 5 cut(s) 905, 965, 1325, 1870, 2198
TspDTI ATGAA 6 cut(s) 81, 116, 245, 1464, 1491, 2087
TspGWI ACGGA 3 cut(s) 1661, 2057, 2217
TspRI CASTG 7 cut(s) 466, 802, 910, 1111, 1675, 1791, 2158
Van91I CCANNNNNTGG 1 cut(s) 563
VpaK11BI GGWCC 2 cut(s) 959, 2204
XagI CCTNNNNNAGG 1 cut(s) 1822
XapI RAATTY 2 cut(s) 1412, 1707
XceI RCATGY 2 cut(s) 19, 2011
XcmI CCANNNNNNNNNTGG 4 cut(s) 113, 403, 466, 564
XhoI CTCGAG 1 cut(s) 317
XmiI GTMKAC 3 cut(s) 161, 1674, 1998
XspI CTAG 2 cut(s) 1704, 1824
ZraI GACGTC 1 cut(s) 1870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.