Prupe.8G091500_v2.0.a1

Pentatricopeptide repeat-containing protein At4g21065-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0022426
Physical Location & Seq
Forward (+)
12450558 .. 12455112
4555 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G091500.1

Sequence Viewer

Length: 1716 bp
ATGGAGATCACCACCATGTCCCAAGCCATGCAGCTCCATGCCCAAATCCTCAAAGCAGGCAACCAGCAAAACCAAAACCTCTCAAAGCTCTTCACGTTCTCTGCTCTGTCTCCCTCCGGAGACCTCTCCTACGCCCGTCTCATTTTAGACTCATTCCCTGCCCCAAACTCTTACTACTACAACACCATGATCCGAGCCTACTCTCAAAGCTCCGACCCGATTCAAGCCCTCCTTCTCTTTCTACCTATGCTTGAAGACCCGACCCACCACACGCCCCGTCCCGACAAGTTCACATACCCTTTCCTCCTCAAGTCCTGTGCCCGTTTGAGACTGACCCATGAAGGAAAACAGATTCATGGGTTGATAGCGAAGTCGGGTCTCGAGTGGGACCGGTATATCCAGCACTCATTGATACATATGTACTGTTTGAGTGGCGAGTCGGGTCTTGCTTATATGGTGTTCGACAGAATGCCTGACAGAGATGTGGTGTCATGGACCTCAATGATTGATGGGCTCGTTGAGGATGGTAGGCCCATCGAGGCAATAAGGTTGTTTGAGCAAATGGTGGATGATGGGGTGGAGGTGAATGATGCGACGGTCGTGTCGGTTTTGAGAGCTTGTGCAGAGACAGGGGCTTTAGGCGTTGGGAGGAGAGTGCATGGGGTTGTTGAAGGTAGAGGGATTGGTTTGAAGGCTAATGTTAATAGTGCCCTTATTGATATGTACGCAAAATGTGCGTGCATAAAGAGTGCATGGCAGGTTTTTGATGACATTGTGGACAAAGATGTTTTTGCATGGACGGCTATGATATCGGGCCTTGCTAGCCATGGTCATTGTCAAGATGCTATTGACTTGTTCAGTAAGATGCAAGGTTTTGGTATTAAACCTGATGAGAGGACAATGACTGCAGTTCTATCGGCGTGTAGGAATGCAGGTCAGGTGGCAGAAGGTTATGCATATTTGAGAAGTATGCAAAATGAATATGGAGTTAGGCCTACAATTCAACACTATGGATGCATGGTAGACCTCCTTGCTCGAGCAGGTCATTTGAAAGAGGCTGAAGAGTTCATCAGAAAAATGCCCATTGAACCTGATGTGGTTATGTGTAGAAACTTGATATGGGCTTGCAAGGTCCACAAGGATACTGAGAGAGCCGAGCGTTTGATAATACATCTTAGGCAGCCAAAGATGGGTTCAAATGATAGCGGAAGTTATGTACTTATTGGCAATGTGTATGCATCAGCGGGAAAATGGCATGACAAGGCAAGGGTTAGAGAATTAATGAAGCAAAAAGGACTAGTAAAACCACCAGGGTTTAGTAGGATTGAGTTAGACGGAGAAATTCATGAATTTGCCGTAGGAGATTCAGGTCACCCGGAAGCAGAAACAATATACAGAAAATTGGAAGAAATAGAAGATAATTTGAGGAAAGAAGGGTATGATCCAAAGCTTTCAGAGGTTTTGCTTGAAATTGATGATGAGGAGAAAGCTTTTCAATTGAGTCACCACAGTGAGAAGCTTGCTCTTGCCTTTGGTCTAATTAGAACAAGTCCGGGGTCTGAAATTCGGATTGTGAAAAACTTGCGATCTTGTGAGAACTGCCATGCTGTTATGAAACTAATTTCAAAGGTATACCAACGAGAGATAATAATGAGGGATCGAATACGATTCCACCATTTCAAGAATGGGGATTGCTCTTGCAGGGATTACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

572

Amino Acids

64.52

Weight (kDa)

6.43

Isoelectric Point (pI)

41.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022426)

Species Orthologous Gene IDs
malus_domestica MD05G1062000.v1.1
prunus_persica Prupe.8G091500_v2.0.a1 Prupe.8G091500_v2.0.a1
pyrus_communis pycom05g05340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 601
Acc36I ACCTGC 3 cut(s) 748, 923, 1031
AccI GTMKAC 2 cut(s) 1023, 1632
AccIII TCCGGA 1 cut(s) 116
AciI CCGC 2 cut(s) 1206, 1244
AclWI GGATC 3 cut(s) 184, 1436, 1665
AcsI RAATTY 3 cut(s) 1341, 1349, 1563
AcuI CTGAAG 1 cut(s) 1080
AfaI GTAC 3 cut(s) 422, 725, 1218
AfiI CCNNNNNNNGG 1 cut(s) 1190
AgeI ACCGGT 1 cut(s) 390
AhlI ACTAGT 1 cut(s) 1297
AjnI CCWGG 1 cut(s) 1309
AjuI GAANNNNNNNTTGG 2 cut(s) 1177, 1209
AleI CACNNNNGTG 1 cut(s) 1509
AluBI AGCT 7 cut(s) 34, 88, 210, 617, 1450, 1490, 1519
AluI AGCT 7 cut(s) 34, 88, 210, 617, 1450, 1490, 1519
Alw26I GTCTC 6 cut(s) 114, 114, 143, 322, 383, 620
AlwI GGATC 3 cut(s) 184, 1436, 1665
Ama87I CYCGRG 2 cut(s) 380, 1035
Aor13HI TCCGGA 1 cut(s) 116
AoxI GGCC 3 cut(s) 530, 814, 992
ApeKI GCWGC 2 cut(s) 31, 1180
ApoI RAATTY 3 cut(s) 1341, 1349, 1563
AseI ATTAAT 1 cut(s) 1280
AsiGI ACCGGT 1 cut(s) 390
AspS9I GGNCC 5 cut(s) 388, 495, 531, 814, 1132
AsuC2I CCSGG 2 cut(s) 1376, 1554
AsuHPI GGTGA 3 cut(s) 595, 1364, 1496
AsuNHI GCTAGC 1 cut(s) 821
AvaI CYCGRG 2 cut(s) 380, 1035
AvaII GGWCC 3 cut(s) 388, 495, 1132
BaeGI GKGCMC 2 cut(s) 322, 712
BaeI ACNNNNGTAYC 2 cut(s) 404, 437
BanII GRGCYC 1 cut(s) 516
BbsI GAAGAC 1 cut(s) 261
BbvI GCAGC 2 cut(s) 43, 1192
BccI CCATC 5 cut(s) 503, 518, 542, 566, 1183
BceAI ACGGC 2 cut(s) 816, 1340
BcgI CGANNNNNNTGC 2 cut(s) 897, 931
BciT130I CCWGG 1 cut(s) 1311
BciVI GTATCC 1 cut(s) 1135
BcnI CCSGG 2 cut(s) 1376, 1554
BcoDI GTCTC 6 cut(s) 114, 114, 143, 322, 383, 620
BcuI ACTAGT 1 cut(s) 1297
BfaI CTAG 2 cut(s) 822, 1298
BfmI CTRYAG 1 cut(s) 906
BfuAI ACCTGC 3 cut(s) 748, 923, 1031
BfuI GTATCC 1 cut(s) 1135
BisI GCNGC 2 cut(s) 32, 1181
BlsI GCNGC 2 cut(s) 33, 1182
Bme1390I CCNGG 3 cut(s) 1311, 1376, 1554
Bme18I GGWCC 3 cut(s) 388, 495, 1132
BmeT110I CYCGRG 2 cut(s) 380, 1035
BmgT120I GGNCC 5 cut(s) 388, 495, 531, 814, 1132
BmiI GGNNCC 1 cut(s) 389
BmrFI CCNGG 3 cut(s) 1311, 1376, 1554
BmsI GCATC 5 cut(s) 580, 832, 855, 1004, 1247
BmtI GCTAGC 1 cut(s) 825
BpiI GAAGAC 1 cut(s) 261
BpuEI CTTGAG 1 cut(s) 293
BpuMI CCSGG 2 cut(s) 1376, 1554
BsaI GGTCTC 2 cut(s) 114, 383
BsaJI CCNNGG 3 cut(s) 826, 1310, 1553
BsaWI WCCGGW 2 cut(s) 116, 390
Bsc4I CCNNNNNNNGG 1 cut(s) 1190
Bse118I RCCGGY 1 cut(s) 390
Bse1I ACTGG 1 cut(s) 1715
Bse3DI GCAATG 1 cut(s) 1234
BseAI TCCGGA 1 cut(s) 116
BseBI CCWGG 1 cut(s) 1311
BseDI CCNNGG 3 cut(s) 826, 1310, 1553
BseGI GGATG 3 cut(s) 529, 574, 1019
BseLI CCNNNNNNNGG 1 cut(s) 1190
BseMI GCAATG 1 cut(s) 1234
BseMII CTCAG 1 cut(s) 1137
BseNI ACTGG 1 cut(s) 1715
BseRI GAGGAG 3 cut(s) 296, 664, 1496
BseSI GKGCMC 2 cut(s) 322, 712
BseXI GCAGC 2 cut(s) 43, 1192
BsgI GTGCAG 1 cut(s) 642
Bsh1285I CGRYCG 1 cut(s) 600
BshFI GGCC 3 cut(s) 532, 816, 994
BshTI ACCGGT 1 cut(s) 390
BsiEI CGRYCG 1 cut(s) 600
BsiHKCI CYCGRG 2 cut(s) 380, 1035
BsiSI CCGG 4 cut(s) 117, 391, 1376, 1553
BslFI GGGAC 3 cut(s) 4, 264, 401
BslI CCNNNNNNNGG 1 cut(s) 1190
BsmAI GTCTC 6 cut(s) 114, 114, 143, 322, 383, 620
BsmBI CGTCTC 1 cut(s) 143
BsmFI GGGAC 3 cut(s) 4, 264, 401
BsmI GAATGC 2 cut(s) 474, 934
BsnI GGCC 3 cut(s) 532, 816, 994
Bso31I GGTCTC 2 cut(s) 114, 383
BsoBI CYCGRG 2 cut(s) 380, 1035
Bsp1286I GDGCHC 3 cut(s) 322, 516, 712
Bsp13I TCCGGA 1 cut(s) 116
Bsp143I GATC 5 cut(s) 6, 189, 1441, 1586, 1657
Bsp19I CCATGG 1 cut(s) 826
BspACI CCGC 2 cut(s) 1206, 1244
BspANI GGCC 3 cut(s) 532, 816, 994
BspCNI CTCAG 1 cut(s) 1138
BspEI TCCGGA 1 cut(s) 116
BspHI TCATGA 1 cut(s) 1345
BspLI GGNNCC 1 cut(s) 389
BspMAI CTGCAG 1 cut(s) 910
BspMI ACCTGC 3 cut(s) 748, 923, 1031
BspOI GCTAGC 1 cut(s) 825
BspPI GGATC 3 cut(s) 184, 1436, 1665
BspQI GCTCTTC 1 cut(s) 95
BspTNI GGTCTC 2 cut(s) 114, 383
BsrDI GCAATG 1 cut(s) 1234
BsrFI RCCGGY 1 cut(s) 390
BsrI ACTGG 1 cut(s) 1715
BssAI RCCGGY 1 cut(s) 390
BssECI CCNNGG 3 cut(s) 826, 1310, 1553
BssMI GATC 5 cut(s) 6, 189, 1441, 1586, 1657
BssNAI GTATAC 1 cut(s) 1633
BssT1I CCWWGG 1 cut(s) 826
Bst1107I GTATAC 1 cut(s) 1633
Bst2UI CCWGG 1 cut(s) 1311
Bst4CI ACNGT 3 cut(s) 425, 598, 1511
Bst6I CTCTTC 2 cut(s) 95, 1056
BstAPI GCANNNNNTGC 1 cut(s) 734
BstC8I GCNNGC 5 cut(s) 58, 739, 823, 1126, 1521
BstDEI CTNAG 2 cut(s) 1146, 1175
BstDSI CCRYGG 1 cut(s) 826
BstEII GGTNACC 1 cut(s) 1370
BstF5I GGATG 3 cut(s) 529, 574, 1019
BstKTI GATC 5 cut(s) 9, 192, 1444, 1589, 1660
BstMAI GTCTC 6 cut(s) 114, 114, 143, 322, 383, 620
BstMBI GATC 5 cut(s) 6, 189, 1441, 1586, 1657
BstMCI CGRYCG 1 cut(s) 600
BstMWI GCNNNNNNNGC 3 cut(s) 734, 800, 822
BstNI CCWGG 1 cut(s) 1311
BstPI GGTNACC 1 cut(s) 1370
BstSCI CCNGG 3 cut(s) 1309, 1374, 1552
BstSFI CTRYAG 1 cut(s) 906
BstSLI GKGCMC 2 cut(s) 322, 712
BstV1I GCAGC 2 cut(s) 43, 1192
BstV2I GAAGAC 1 cut(s) 261
BstZ17I GTATAC 1 cut(s) 1633
BsuI GTATCC 1 cut(s) 1135
BsuRI GGCC 3 cut(s) 532, 816, 994
BtgI CCRYGG 1 cut(s) 826
BtsCI GGATG 3 cut(s) 529, 574, 1019
BtsIMutI CAGTG 1 cut(s) 1516
BveI ACCTGC 3 cut(s) 748, 923, 1031
Cac8I GCNNGC 5 cut(s) 58, 739, 823, 1126, 1521
CciI TCATGA 1 cut(s) 1345
Cfr10I RCCGGY 1 cut(s) 390
Cfr13I GGNCC 5 cut(s) 388, 495, 531, 814, 1132
Csp6I GTAC 3 cut(s) 421, 724, 1217
CspAI ACCGGT 1 cut(s) 390
CviQI GTAC 3 cut(s) 421, 724, 1217
DdeI CTNAG 2 cut(s) 1146, 1175
DpnI GATC 5 cut(s) 8, 191, 1443, 1588, 1659
DpnII GATC 5 cut(s) 6, 189, 1441, 1586, 1657
DrdI GACNNNNNNGTC 1 cut(s) 601
DseDI GACNNNNNNGTC 1 cut(s) 601
Eam1104I CTCTTC 2 cut(s) 95, 1056
EarI CTCTTC 2 cut(s) 95, 1056
Eco130I CCWWGG 1 cut(s) 826
Eco147I AGGCCT 1 cut(s) 994
Eco24I GRGCYC 1 cut(s) 516
Eco31I GGTCTC 2 cut(s) 114, 383
Eco32I GATATC 1 cut(s) 810
Eco47I GGWCC 3 cut(s) 388, 495, 1132
Eco57I CTGAAG 1 cut(s) 1080
Eco88I CYCGRG 2 cut(s) 380, 1035
Eco91I GGTNACC 1 cut(s) 1370
EcoO65I GGTNACC 1 cut(s) 1370
EcoRII CCWGG 1 cut(s) 1309
EcoRV GATATC 1 cut(s) 810
EcoT14I CCWWGG 1 cut(s) 826
EcoT22I ATGCAT 3 cut(s) 958, 1019, 1240
EcoT38I GRGCYC 1 cut(s) 516
ErhI CCWWGG 1 cut(s) 826
Esp3I CGTCTC 1 cut(s) 143
FalI AAGNNNNNCTT 2 cut(s) 216, 248
FaqI GGGAC 3 cut(s) 4, 264, 401
FauI CCCGC 1 cut(s) 1237
FauNDI CATATG 1 cut(s) 417
FblI GTMKAC 2 cut(s) 1023, 1632
Fnu4HI GCNGC 2 cut(s) 32, 1181
FokI GGATG 3 cut(s) 536, 581, 1026
FriOI GRGCYC 1 cut(s) 516
Fsp4HI GCNGC 2 cut(s) 32, 1181
FspBI CTAG 2 cut(s) 822, 1298
GluI GCNGC 2 cut(s) 32, 1181
HaeIII GGCC 3 cut(s) 532, 816, 994
HapII CCGG 4 cut(s) 117, 391, 1376, 1553
HindIII AAGCTT 3 cut(s) 1448, 1488, 1517
HinfI GANTC 7 cut(s) 149, 220, 352, 437, 1364, 1501, 1668
HpaII CCGG 4 cut(s) 117, 391, 1376, 1553
HphI GGTGA 3 cut(s) 595, 1364, 1496
Hpy166II GTNNAC 5 cut(s) 291, 778, 1024, 1135, 1633
Hpy188I TCNGA 6 cut(s) 194, 214, 1073, 1456, 1561, 1569
Hpy188III TCNNGA 6 cut(s) 117, 281, 380, 839, 1346, 1681
Hpy8I GTNNAC 5 cut(s) 291, 778, 1024, 1135, 1633
Hpy99I CGWCG 1 cut(s) 598
HpyAV CCTTC 6 cut(s) 242, 335, 665, 685, 941, 1427
HpyCH4III ACNGT 3 cut(s) 425, 598, 1511
HpyCH4IV ACGT 1 cut(s) 95
HpyF10VI GCNNNNNNNGC 3 cut(s) 734, 800, 822
HpyF3I CTNAG 2 cut(s) 1146, 1175
HpySE526I ACGT 1 cut(s) 95
Kpn2I TCCGGA 1 cut(s) 116
Kzo9I GATC 5 cut(s) 6, 189, 1441, 1586, 1657
LguI GCTCTTC 1 cut(s) 95
LmnI GCTCC 2 cut(s) 39, 215
Lsp1109I GCAGC 2 cut(s) 43, 1192
LweI GCATC 5 cut(s) 580, 832, 855, 1004, 1247
MaeI CTAG 2 cut(s) 822, 1298
MaeII ACGT 1 cut(s) 95
MaeIII GTNAC 2 cut(s) 1370, 1502
MalI GATC 5 cut(s) 8, 191, 1443, 1588, 1659
MboI GATC 5 cut(s) 6, 189, 1441, 1586, 1657
MboII GAAGA 5 cut(s) 82, 266, 1073, 1418, 1427
MfeI CAATTG 1 cut(s) 1496
MhlI GDGCHC 3 cut(s) 322, 516, 712
MlyI GAGTC 3 cut(s) 143, 446, 1510
MmeI TCCRAC 1 cut(s) 237
Mph1103I ATGCAT 3 cut(s) 958, 1019, 1240
MroI TCCGGA 1 cut(s) 116
MseI TTAA 3 cut(s) 702, 882, 1280
MslI CAYNNNNRTG 2 cut(s) 14, 1509
MspA1I CMGCKG 1 cut(s) 1244
MspI CCGG 4 cut(s) 117, 391, 1376, 1553
MspR9I CCNGG 3 cut(s) 1311, 1376, 1554
MunI CAATTG 1 cut(s) 1496
Mva1269I GAATGC 2 cut(s) 474, 934
MvaI CCWGG 1 cut(s) 1311
MwoI GCNNNNNNNGC 3 cut(s) 734, 800, 822
NciI CCSGG 2 cut(s) 1376, 1554
NcoI CCATGG 1 cut(s) 826
NdeI CATATG 1 cut(s) 417
NdeII GATC 5 cut(s) 6, 189, 1441, 1586, 1657
NheI GCTAGC 1 cut(s) 821
NlaIV GGNNCC 1 cut(s) 389
NmeAIII GCCGAG 1 cut(s) 1180
NmuCI GTSAC 2 cut(s) 1370, 1502
NsiI ATGCAT 3 cut(s) 958, 1019, 1240
OliI CACNNNNGTG 1 cut(s) 1509
PaeR7I CTCGAG 2 cut(s) 380, 1035
PagI TCATGA 1 cut(s) 1345
PceI AGGCCT 1 cut(s) 994
PciSI GCTCTTC 1 cut(s) 95
PctI GAATGC 2 cut(s) 474, 934
PfeI GAWTC 4 cut(s) 220, 352, 1364, 1668
PinAI ACCGGT 1 cut(s) 390
PkrI GCNGC 2 cut(s) 33, 1182
PleI GAGTC 3 cut(s) 143, 445, 1509
PpsI GAGTC 3 cut(s) 143, 445, 1509
PshBI ATTAAT 1 cut(s) 1280
Psp6I CCWGG 1 cut(s) 1309
PspEI GGTNACC 1 cut(s) 1370
PspGI CCWGG 1 cut(s) 1309
PspN4I GGNNCC 1 cut(s) 389
PspPI GGNCC 5 cut(s) 388, 495, 531, 814, 1132
PspXI VCTCGAGB 1 cut(s) 1035
PstI CTGCAG 1 cut(s) 910
RsaI GTAC 3 cut(s) 422, 725, 1218
RsaNI GTAC 3 cut(s) 421, 724, 1217
RseI CAYNNNNRTG 2 cut(s) 14, 1509
SapI GCTCTTC 1 cut(s) 95
SaqAI TTAA 3 cut(s) 702, 882, 1280
SatI GCNGC 2 cut(s) 32, 1181
Sau3AI GATC 5 cut(s) 6, 189, 1441, 1586, 1657
Sau96I GGNCC 5 cut(s) 388, 495, 531, 814, 1132
SchI GAGTC 3 cut(s) 143, 446, 1510
ScrFI CCNGG 3 cut(s) 1311, 1376, 1554
SduI GDGCHC 3 cut(s) 322, 516, 712
SfaNI GCATC 5 cut(s) 580, 832, 855, 1004, 1247
SfcI CTRYAG 1 cut(s) 906
Sfr274I CTCGAG 2 cut(s) 380, 1035
SinI GGWCC 3 cut(s) 388, 495, 1132
SlaI CTCGAG 2 cut(s) 380, 1035
SmiMI CAYNNNNRTG 2 cut(s) 14, 1509
SmlI CTYRAG 3 cut(s) 308, 380, 1035
SmoI CTYRAG 3 cut(s) 308, 380, 1035
SpeI ACTAGT 1 cut(s) 1297
SseBI AGGCCT 1 cut(s) 994
SsiI CCGC 2 cut(s) 1206, 1244
SspMI CTAG 2 cut(s) 822, 1298
StuI AGGCCT 1 cut(s) 994
StyD4I CCNGG 3 cut(s) 1309, 1374, 1552
StyI CCWWGG 1 cut(s) 826
TaaI ACNGT 3 cut(s) 425, 598, 1511
TaiI ACGT 1 cut(s) 98
TaqI TCGA 5 cut(s) 381, 462, 537, 1036, 1660
TatI WGTACW 2 cut(s) 420, 1216
TfiI GAWTC 4 cut(s) 220, 352, 1364, 1668
Tru1I TTAA 3 cut(s) 702, 882, 1280
Tru9I TTAA 3 cut(s) 702, 882, 1280
TscAI CASTG 1 cut(s) 1516
TseFI GTSAC 2 cut(s) 1370, 1502
TseI GCWGC 2 cut(s) 31, 1180
Tsp45I GTSAC 2 cut(s) 1370, 1502
TspDTI ATGAA 8 cut(s) 344, 354, 993, 1057, 1298, 1334, 1362, 1628
TspGWI ACGGA 1 cut(s) 1350
TspRI CASTG 1 cut(s) 1516
VpaK11BI GGWCC 3 cut(s) 388, 495, 1132
VspI ATTAAT 1 cut(s) 1280
XapI RAATTY 3 cut(s) 1341, 1349, 1563
XcmI CCANNNNNNNNNTGG 1 cut(s) 1682
XhoI CTCGAG 2 cut(s) 380, 1035
XmiI GTMKAC 2 cut(s) 1023, 1632
XspI CTAG 2 cut(s) 822, 1298
Zsp2I ATGCAT 3 cut(s) 958, 1019, 1240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.