Prupe.8G269200_v2.0.a1

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0028969
Physical Location & Seq
Reverse (-)
22392556 .. 22395841
3286 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G269200.1

Sequence Viewer

Length: 1818 bp
ATGAGGAAAGATGATAAATGGGGTGGTGCTCCGGTGGAGATAGAGGCAGAGCCAGCGACGACGTCGGATGAGGTGAAGCAAGGTTGGCTGTTGAAGCCAGAAAATAAAGAAAATGGTGGTCACGACCATGAGAGGAATATTGATGGGAAAAAGAAGAAGAGGAGGATGATGAGCAAGATTTTGGGGAGCTTGGTGAGCATAAGGAGCCTAGTGGTGGTTGGGGTTGCCATTCTGGTGGCCTTGATCATTGTCCTAGTTAAGATTGCTCAACGCAACCACCATCATCTGAACTCTTCATCACCTCACAACTATACCACCGCCCTTCACCAAGCGCTCCTCTTTTTCAACGCCCAACGCTCTGGAAAACTGCCAAAGGACAACAATGTTTCATGGAGAGGTGATTCCTGCTTGACTGATGGCAACTCTAACACCAAAACTCTGGTTGGTGGATATTACGACGCTGGAGACGCAAGCAAGTTGAGCTTCCCTGCATCTTTTGCCATGACCATGTTGAGCTGGAGTGTGATCGAATACAGTGCAAAATATGAAGCACTAGGAGAGCTAGACCATGTCAAGGGCATCATCAAGTGGGGCACAGATTACCTTCTCAACACCTTCAATTCCTCTGCTCATTCCATTGCCAATGTTGTTGCAGATGTTAGGGGAGGAGATCATGATGCTTACTCTCACGACTGTTGGATACGTCCTGAAGATATTGACCATCAAAGAACTGGACAAGAGTGTTATAACTGCCCAGCCCTCGCTGCAGAAATGGCTTCTGCTTTAGCTTCTGCATCCATTGTTTTCAAAGATAACATTAAGTACTCACAGAAACTTGTTCATGGTGCTGATTTACTCTTTAAATTTGCTACCAAACAGCAAGGTGCAAAGTATGCGGACGGTGCAGACCCGTCGTCCCCTCTCTACAATTCTACTGGTTTCTGGGATGAATTTCTGTGGGGGGGATCGTGGTTGTATCTTGCAACAGGGAATTTATCCTATCTTCAACTTGTTACTAACCCTGACTTGGTTGGGCATGAAAGGGCTTTGTGGACCGACTCTGATAATAGGGTACTTAGCTGGGACAACAAGCATGCTGGTGCTTTTTTGCTTCTAAGCCGACTGAGATTGTTCCTGAGTTACGGCTACCCCTACGAAGAGATGTTGAAAAAATTTCACAGCCAGACTGAAGAAACAATGTGCTCCTACCTTCCTGTTTTCTCAAGCTTCAGAAGAACAAAAGGAGGGCTGATTCAGTTGAACCATGGAAGGCCAAAACCTCTTCAGTATGTAGTCTATGCAGCATTTTTGGCTCAACTATATAGTGACTACATTGACGCTATTTCTGCTCCTGGATGGTATTGTGGACCCACATTCTACTCCAATGTAGAACTGCGCAGGTTTGCCAGGACCCAAATTGATTACATTCTTGGCAAAAATCCTCGGGGTATAAGCTATGTTGTAGGCTTCAGTGAACATTTTCCACAGCATGTGCACCATAGAGGGGCATCCATTCCCCACAATAAGATCAAGTATGGTTGTAAAGGAGGATTGAAATGGAAAAACAGTCGGACACCGAATCCAAATACAATTGTCGGGGCCATGGTTGCTGGTCCTGATAAGCATGACAATTTCCAGGATGTCCGTTTCAATTACAACTACACTGAGCCGAGCCTTGTAGGAAATGCAGGTCTTGTTGCTGCACTTGTGGCACTATCCAGTGGAACCCCTGCTGGTATTGACAGAAACTCAATGTTCTATGCAGTTCCTCCCCTGACTTCACCCCCACCACCTCCTCCATCACCTTGGATACCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

606

Amino Acids

67.22

Weight (kDa)

8.66

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028969)

Species Orthologous Gene IDs
prunus_persica Prupe.8G269200_v2.0.a1
pyrus_communis pycom11g27700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 747
AatII GACGTC 1 cut(s) 65
Acc16I TGCGCA 1 cut(s) 1397
Acc36I ACCTGC 2 cut(s) 1389, 1679
AciI CCGC 2 cut(s) 318, 896
AclWI GGATC 1 cut(s) 973
AcsI RAATTY 4 cut(s) 863, 950, 991, 1172
AcuI CTGAAG 5 cut(s) 729, 1209, 1213, 1268, 1453
AcyI GRCGYC 1 cut(s) 62
AfaI GTAC 2 cut(s) 824, 1074
AfeI AGCGCT 1 cut(s) 333
AfiI CCNNNNNNNGG 4 cut(s) 214, 574, 1027, 1504
AgsI TTSAA 9 cut(s) 94, 346, 619, 808, 1007, 1168, 1261, 1555, 1651
AhdI GACNNNNNGTC 1 cut(s) 913
AjnI CCWGG 3 cut(s) 1351, 1406, 1635
AluBI AGCT 8 cut(s) 189, 483, 516, 562, 788, 1080, 1227, 1455
AluI AGCT 8 cut(s) 189, 483, 516, 562, 788, 1080, 1227, 1455
Alw21I GWGCWC 3 cut(s) 31, 1205, 1497
Alw26I GTCTC 1 cut(s) 459
Alw44I GTGCAC 1 cut(s) 1493
AlwI GGATC 1 cut(s) 973
Ama87I CYCGRG 1 cut(s) 1443
Aor51HI AGCGCT 1 cut(s) 333
AoxI GGCC 3 cut(s) 237, 1271, 1599
ApaLI GTGCAC 1 cut(s) 1493
ApeKI GCWGC 3 cut(s) 764, 1301, 1700
ApoI RAATTY 4 cut(s) 863, 950, 991, 1172
Asp700I GAANNNNTTC 1 cut(s) 1479
AspLEI GCGC 2 cut(s) 334, 1398
AspS9I GGNCC 5 cut(s) 1053, 1367, 1410, 1599, 1613
AsuHPI GGTGA 7 cut(s) 85, 205, 291, 317, 410, 1773, 1794
AvaI CYCGRG 1 cut(s) 1443
AvaII GGWCC 4 cut(s) 1053, 1367, 1410, 1613
BaeGI GKGCMC 2 cut(s) 596, 1497
Bbv12I GWGCWC 3 cut(s) 31, 1205, 1497
BbvI GCAGC 3 cut(s) 751, 1313, 1687
BccI CCATC 6 cut(s) 137, 288, 410, 729, 1350, 1807
BceAI ACGGC 1 cut(s) 1159
BcgI CGANNNNNNTGC 4 cut(s) 518, 552, 894, 928
BciT130I CCWGG 3 cut(s) 1353, 1408, 1637
BciVI GTATCC 2 cut(s) 693, 1803
BclI TGATCA 1 cut(s) 243
BcoDI GTCTC 1 cut(s) 459
BfaI CTAG 4 cut(s) 209, 254, 554, 563
BfmI CTRYAG 1 cut(s) 765
BfoI RGCGCY 1 cut(s) 335
BfuAI ACCTGC 2 cut(s) 1389, 1679
BfuI GTATCC 2 cut(s) 693, 1803
BisI GCNGC 3 cut(s) 765, 1302, 1701
BlsI GCNGC 3 cut(s) 766, 1303, 1702
BmcAI AGTACT 1 cut(s) 824
Bme1390I CCNGG 3 cut(s) 1353, 1408, 1637
Bme18I GGWCC 4 cut(s) 1053, 1367, 1410, 1613
BmeRI GACNNNNNGTC 1 cut(s) 913
BmeT110I CYCGRG 1 cut(s) 1443
BmgT120I GGNCC 5 cut(s) 1053, 1367, 1410, 1599, 1613
BmiI GGNNCC 5 cut(s) 206, 1369, 1412, 1600, 1726
BmrFI CCNGG 3 cut(s) 1353, 1408, 1637
BmsI GCATC 5 cut(s) 500, 588, 667, 803, 1517
BpmI CTGGAG 2 cut(s) 483, 538
BpuEI CTTGAG 1 cut(s) 1207
BsaHI GRCGYC 1 cut(s) 62
BsaJI CCNNGG 4 cut(s) 1264, 1442, 1602, 1805
BsaWI WCCGGW 1 cut(s) 31
Bsc4I CCNNNNNNNGG 4 cut(s) 214, 574, 1027, 1504
Bse1I ACTGG 3 cut(s) 736, 940, 1719
Bse3DI GCAATG 1 cut(s) 636
BseBI CCWGG 3 cut(s) 1353, 1408, 1637
BseDI CCNNGG 4 cut(s) 1264, 1442, 1602, 1805
BseGI GGATG 7 cut(s) 73, 171, 794, 952, 1361, 1508, 1645
BseLI CCNNNNNNNGG 4 cut(s) 214, 574, 1027, 1504
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 3 cut(s) 1115, 1127, 1656
BseNI ACTGG 3 cut(s) 736, 940, 1719
BseRI GAGGAG 4 cut(s) 175, 326, 681, 1785
BseSI GKGCMC 2 cut(s) 596, 1497
BseXI GCAGC 3 cut(s) 751, 1313, 1687
BseYI CCCAGC 2 cut(s) 754, 1080
BsgI GTGCAG 2 cut(s) 924, 1686
BshFI GGCC 3 cut(s) 239, 1273, 1601
BsiHKAI GWGCWC 3 cut(s) 31, 1205, 1497
BsiHKCI CYCGRG 1 cut(s) 1443
BsiSI CCGG 1 cut(s) 32
BslFI GGGAC 2 cut(s) 901, 1097
BslI CCNNNNNNNGG 4 cut(s) 214, 574, 1027, 1504
BsmAI GTCTC 1 cut(s) 459
BsmBI CGTCTC 1 cut(s) 459
BsmFI GGGAC 2 cut(s) 901, 1097
BsnI GGCC 3 cut(s) 239, 1273, 1601
BsoBI CYCGRG 1 cut(s) 1443
Bsp1286I GDGCHC 4 cut(s) 31, 596, 1205, 1497
Bsp143I GATC 5 cut(s) 243, 525, 670, 965, 1527
Bsp19I CCATGG 2 cut(s) 1264, 1602
BspACI CCGC 2 cut(s) 318, 896
BspANI GGCC 3 cut(s) 239, 1273, 1601
BspCNI CTCAG 3 cut(s) 1116, 1128, 1657
BspHI TCATGA 1 cut(s) 673
BspLI GGNNCC 5 cut(s) 206, 1369, 1412, 1600, 1726
BspMAI CTGCAG 1 cut(s) 769
BspMI ACCTGC 2 cut(s) 1389, 1679
BspPI GGATC 1 cut(s) 973
BsrDI GCAATG 1 cut(s) 636
BsrI ACTGG 3 cut(s) 736, 940, 1719
BssECI CCNNGG 4 cut(s) 1264, 1442, 1602, 1805
BssMI GATC 5 cut(s) 243, 525, 670, 965, 1527
BssNI GRCGYC 1 cut(s) 62
BssT1I CCWWGG 3 cut(s) 1264, 1602, 1805
Bst2UI CCWGG 3 cut(s) 1353, 1408, 1637
Bst4CI ACNGT 4 cut(s) 536, 695, 902, 1568
Bst6I CTCTTC 4 cut(s) 152, 298, 1152, 1287
BstACI GRCGYC 1 cut(s) 62
BstAPI GCANNNNNTGC 2 cut(s) 497, 893
BstC8I GCNNGC 3 cut(s) 54, 472, 1095
BstDEI CTNAG 5 cut(s) 1076, 1115, 1124, 1136, 1665
BstDSI CCRYGG 2 cut(s) 1264, 1602
BstF5I GGATG 7 cut(s) 73, 171, 794, 952, 1361, 1508, 1645
BstH2I RGCGCY 1 cut(s) 335
BstHHI GCGC 2 cut(s) 334, 1398
BstKTI GATC 5 cut(s) 246, 528, 673, 968, 1530
BstMAI GTCTC 1 cut(s) 459
BstMBI GATC 5 cut(s) 243, 525, 670, 965, 1527
BstNI CCWGG 3 cut(s) 1353, 1408, 1637
BstNSI RCATGY 2 cut(s) 1097, 1493
BstSCI CCNGG 3 cut(s) 1351, 1406, 1635
BstSFI CTRYAG 1 cut(s) 765
BstSLI GKGCMC 2 cut(s) 596, 1497
BstV1I GCAGC 3 cut(s) 751, 1313, 1687
BstXI CCANNNNNNTGG 4 cut(s) 235, 359, 439, 1806
BsuI GTATCC 2 cut(s) 693, 1803
BsuRI GGCC 3 cut(s) 239, 1273, 1601
BtgI CCRYGG 2 cut(s) 1264, 1602
BtsCI GGATG 7 cut(s) 73, 171, 794, 952, 1361, 1508, 1645
BtsIMutI CAGTG 4 cut(s) 541, 1477, 1662, 1726
BveI ACCTGC 2 cut(s) 1389, 1679
Cac8I GCNNGC 3 cut(s) 54, 472, 1095
CciI TCATGA 1 cut(s) 673
CfoI GCGC 2 cut(s) 334, 1398
Cfr13I GGNCC 5 cut(s) 1053, 1367, 1410, 1599, 1613
CseI GACGC 3 cut(s) 467, 476, 1346
Csp6I GTAC 2 cut(s) 823, 1073
CviQI GTAC 2 cut(s) 823, 1073
DdeI CTNAG 5 cut(s) 1076, 1115, 1124, 1136, 1665
DpnI GATC 5 cut(s) 245, 527, 672, 967, 1529
DpnII GATC 5 cut(s) 243, 525, 670, 965, 1527
DraI TTTAAA 1 cut(s) 862
DriI GACNNNNNGTC 1 cut(s) 913
Eam1104I CTCTTC 4 cut(s) 152, 298, 1152, 1287
Eam1105I GACNNNNNGTC 1 cut(s) 913
EarI CTCTTC 4 cut(s) 152, 298, 1152, 1287
Eco130I CCWWGG 3 cut(s) 1264, 1602, 1805
Eco47I GGWCC 4 cut(s) 1053, 1367, 1410, 1613
Eco47III AGCGCT 1 cut(s) 333
Eco57I CTGAAG 5 cut(s) 729, 1209, 1213, 1268, 1453
Eco88I CYCGRG 1 cut(s) 1443
EcoO109I RGGNCCY 1 cut(s) 1410
EcoRII CCWGG 3 cut(s) 1351, 1406, 1635
EcoT14I CCWWGG 3 cut(s) 1264, 1602, 1805
ErhI CCWWGG 3 cut(s) 1264, 1602, 1805
Esp3I CGTCTC 1 cut(s) 459
FalI AAGNNNNNCTT 2 cut(s) 467, 499
FaqI GGGAC 2 cut(s) 901, 1097
FbaI TGATCA 1 cut(s) 243
Fnu4HI GCNGC 3 cut(s) 765, 1302, 1701
FokI GGATG 7 cut(s) 80, 178, 781, 959, 1368, 1495, 1652
Fsp4HI GCNGC 3 cut(s) 765, 1302, 1701
FspBI CTAG 4 cut(s) 209, 254, 554, 563
FspI TGCGCA 1 cut(s) 1397
GlaI GCGC 2 cut(s) 333, 1397
GluI GCNGC 3 cut(s) 765, 1302, 1701
GsaI CCCAGC 2 cut(s) 758, 1084
GsuI CTGGAG 2 cut(s) 483, 538
HaeII RGCGCY 1 cut(s) 335
HaeIII GGCC 3 cut(s) 239, 1273, 1601
HapII CCGG 1 cut(s) 32
HgaI GACGC 3 cut(s) 467, 476, 1346
HhaI GCGC 2 cut(s) 334, 1398
Hin1I GRCGYC 1 cut(s) 62
Hin6I GCGC 2 cut(s) 332, 1396
HinP1I GCGC 2 cut(s) 332, 1396
HindIII AAGCTT 1 cut(s) 1225
HinfI GANTC 4 cut(s) 401, 1058, 1252, 1579
HpaII CCGG 1 cut(s) 32
HphI GGTGA 7 cut(s) 85, 205, 291, 317, 410, 1773, 1794
Hpy166II GTNNAC 4 cut(s) 1053, 1367, 1475, 1495
Hpy188I TCNGA 5 cut(s) 67, 288, 1063, 1232, 1572
Hpy188III TCNNGA 7 cut(s) 122, 360, 674, 689, 707, 1135, 1616
Hpy8I GTNNAC 4 cut(s) 1053, 1367, 1475, 1495
Hpy99I CGWCG 5 cut(s) 61, 64, 67, 461, 916
HpyAV CCTTC 5 cut(s) 332, 614, 625, 1220, 1263
HpyCH4III ACNGT 4 cut(s) 536, 695, 902, 1568
HpyCH4IV ACGT 2 cut(s) 62, 703
HpyF3I CTNAG 5 cut(s) 1076, 1115, 1124, 1136, 1665
HpySE526I ACGT 2 cut(s) 62, 703
Hsp92I GRCGYC 1 cut(s) 62
HspAI GCGC 2 cut(s) 332, 1396
Ksp22I TGATCA 1 cut(s) 243
Kzo9I GATC 5 cut(s) 243, 525, 670, 965, 1527
LmnI GCTCC 6 cut(s) 34, 186, 204, 339, 1208, 1354
Lsp1109I GCAGC 3 cut(s) 751, 1313, 1687
LweI GCATC 5 cut(s) 500, 588, 667, 803, 1517
MaeI CTAG 4 cut(s) 209, 254, 554, 563
MaeII ACGT 2 cut(s) 62, 703
MaeIII GTNAC 4 cut(s) 119, 1012, 1139, 1325
MalI GATC 5 cut(s) 245, 527, 672, 967, 1529
MboI GATC 5 cut(s) 243, 525, 670, 965, 1527
MboII GAAGA 9 cut(s) 166, 169, 285, 722, 995, 1169, 1202, 1245, 1274
MfeI CAATTG 1 cut(s) 1590
MhlI GDGCHC 4 cut(s) 31, 596, 1205, 1497
MlyI GAGTC 1 cut(s) 1052
MmeI TCCRAC 3 cut(s) 45, 677, 1550
MroXI GAANNNNTTC 1 cut(s) 1479
MseI TTAA 3 cut(s) 258, 819, 861
MslI CAYNNNNRTG 4 cut(s) 126, 233, 506, 1098
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 3 cut(s) 1353, 1408, 1637
MunI CAATTG 1 cut(s) 1590
MvaI CCWGG 3 cut(s) 1353, 1408, 1637
NcoI CCATGG 2 cut(s) 1264, 1602
NdeII GATC 5 cut(s) 243, 525, 670, 965, 1527
NlaIV GGNNCC 5 cut(s) 206, 1369, 1412, 1600, 1726
NmeAIII GCCGAG 1 cut(s) 1695
NmuCI GTSAC 2 cut(s) 119, 1325
NsbI TGCGCA 1 cut(s) 1397
NspI RCATGY 2 cut(s) 1097, 1493
PaeI GCATGC 1 cut(s) 1097
PagI TCATGA 1 cut(s) 673
PdmI GAANNNNTTC 1 cut(s) 1479
PfeI GAWTC 3 cut(s) 401, 1252, 1579
PflFI GACNNNGTC 2 cut(s) 61, 569
PfoI TCCNGGA 2 cut(s) 1351, 1635
PkrI GCNGC 3 cut(s) 766, 1303, 1702
PleI GAGTC 1 cut(s) 1052
PpsI GAGTC 1 cut(s) 1052
PpuMI RGGWCCY 1 cut(s) 1410
PsiI TTATAA 1 cut(s) 747
Psp5II RGGWCCY 1 cut(s) 1410
Psp6I CCWGG 3 cut(s) 1351, 1406, 1635
PspFI CCCAGC 2 cut(s) 754, 1080
PspGI CCWGG 3 cut(s) 1351, 1406, 1635
PspN4I GGNNCC 5 cut(s) 206, 1369, 1412, 1600, 1726
PspPI GGNCC 5 cut(s) 1053, 1367, 1410, 1599, 1613
PspPPI RGGWCCY 1 cut(s) 1410
PstI CTGCAG 1 cut(s) 769
PsyI GACNNNGTC 2 cut(s) 61, 569
RsaI GTAC 2 cut(s) 824, 1074
RsaNI GTAC 2 cut(s) 823, 1073
RseI CAYNNNNRTG 4 cut(s) 126, 233, 506, 1098
SaqAI TTAA 3 cut(s) 258, 819, 861
SatI GCNGC 3 cut(s) 765, 1302, 1701
Sau3AI GATC 5 cut(s) 243, 525, 670, 965, 1527
Sau96I GGNCC 5 cut(s) 1053, 1367, 1410, 1599, 1613
ScaI AGTACT 1 cut(s) 824
SchI GAGTC 1 cut(s) 1052
ScrFI CCNGG 3 cut(s) 1353, 1408, 1637
SduI GDGCHC 4 cut(s) 31, 596, 1205, 1497
SfaNI GCATC 5 cut(s) 500, 588, 667, 803, 1517
SfcI CTRYAG 1 cut(s) 765
SinI GGWCC 4 cut(s) 1053, 1367, 1410, 1613
SmiMI CAYNNNNRTG 4 cut(s) 126, 233, 506, 1098
SmlI CTYRAG 1 cut(s) 1222
SmoI CTYRAG 1 cut(s) 1222
SphI GCATGC 1 cut(s) 1097
SsiI CCGC 2 cut(s) 318, 896
SspI AATATT 1 cut(s) 139
SspMI CTAG 4 cut(s) 209, 254, 554, 563
StyD4I CCNGG 3 cut(s) 1351, 1406, 1635
StyI CCWWGG 3 cut(s) 1264, 1602, 1805
TaaI ACNGT 4 cut(s) 536, 695, 902, 1568
TaiI ACGT 2 cut(s) 65, 706
TaqI TCGA 1 cut(s) 528
TaqII GACCGA 1 cut(s) 1070
TatI WGTACW 1 cut(s) 822
TfiI GAWTC 3 cut(s) 401, 1252, 1579
Tru1I TTAA 3 cut(s) 258, 819, 861
Tru9I TTAA 3 cut(s) 258, 819, 861
TscAI CASTG 4 cut(s) 541, 1477, 1669, 1726
TseFI GTSAC 2 cut(s) 119, 1325
TseI GCWGC 3 cut(s) 764, 1301, 1700
Tsp45I GTSAC 2 cut(s) 119, 1325
TspDTI ATGAA 6 cut(s) 285, 378, 561, 830, 963, 1053
TspGWI ACGGA 1 cut(s) 1634
TspRI CASTG 4 cut(s) 541, 1477, 1669, 1726
Tth111I GACNNNGTC 2 cut(s) 61, 569
VneI GTGCAC 1 cut(s) 1493
VpaK11BI GGWCC 4 cut(s) 1053, 1367, 1410, 1613
XapI RAATTY 4 cut(s) 863, 950, 991, 1172
XceI RCATGY 2 cut(s) 1097, 1493
XcmI CCANNNNNNNNNTGG 1 cut(s) 728
XmnI GAANNNNTTC 1 cut(s) 1479
XspI CTAG 4 cut(s) 209, 254, 554, 563
ZraI GACGTC 1 cut(s) 63
ZrmI AGTACT 1 cut(s) 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.