pycom01g00150

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0019312
Physical Location & Seq
Forward (+)
343892 .. 345691
1800 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g00150.3

Sequence Viewer

Length: 1431 bp
ATGGTGCAGGATCACCCAAGGCCACTGTTTCAGCGCCCCGTTGTCGTTGAAGTGCAATGGGCATTCGTCCGCCCATGCGCATTGGAGAAGTTGGTTCTTGTGCTTGTGATGAACGTATCGACGACAATGATGGAGTACCAGAGAACGGCGGAGCCTTCCTCTTCATCCTCACATCGGAAACCACTGCAATCCTCTGACCATGTTGAAGACATTTCCTGTTGCAGGAGCTGTGTGAACTGTCGAATCGAGACCCAAGTCAGGTTCAGAAGGAGAGACCTGATGGGGGTCATTAAATCAGTAAATTTGGGGATCGTGAAAATTAGGGCTTGTGGCATAGGAATGAGGGATGTGAGAGACGTCTATGGAGTGCGAATACAAGTGGAATGTAAAATCGTACTACAAATATTTTACAAAGAGAGATCGATCCAAATAGGTAACCTATCCTACATTCTAGGTTTTACAGATATTCACAAATCAATTATAAACAATTTACAAAATAGTTTCCTAAAACTAAGCCAAGATATCAGGGATGATATCATTGACTTCTCAACACTCCCCCTCAACTTTGAGAAAGTATCCTTTCCTAATGGCTTCGTGAGTACATCTCTTGTCCCGGATGAAGTGACAGTCCATTTCTATATGCCTGGTTCGCTCATGAAAAACTGGGTTAGCAGCAATGTGAAGAGCTGCTTTATTGTCGCAATGGATAAACATCGTAGCCATGATAATTCACAACAAGCTCCAGCCATGGCTCGATACTCGGCCTCTGCTGATGACAAAGACACAGTCTTTTGTCTCTTTGACTTCCAAGAAACCAATGAAGCGCCCAAAAAAATACAATACCCCGTAGTTGATCTTCGTGTTGTTGGGCAACCTGCCCAGTCCGAGTCACAATATGCATTTAAGACTAAATCATTTTCCGCAGAGAAGAACAAACCTTGTCCAGGTGTATTTTTGATATATTTCAAAATCCGAAGAGCTGCTTCCATGTGTGGTGTTATATCCGGCCTAGTGATTGTTAGGTATATCAGACGCCCCACTAATCTTCTATATTTTGCCGGGTCTTTGAGGATTGCACCATTGTCTGATAATTTAAGATTCTGTTCCATGGGAAAGTCGACGGGGCGAGCTCCCAAAAGACCTCCATCTTTCAATATTTCTAAAGCATATTTCCGTTGGGAAATAAAGATCCCTTTCTTAGAACGTGATACTTCAATCCCAAGAAAGTACTTCAAATTACCAAGATCCTTGATTTTAAAACGACCATTAAGGAATTGTTTAAGGGCATCAATAGTACTCGAATCATTTCCCGTAATCAAAATAAAGAGTAGTCGGACTTGGATTGTACATAACCAGCGGATTGAATGGCCTCAGAAAACTTCGCAAACCATTGACGAGAGGCTTGTTTCAACCCATATAAAGACTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

477

Amino Acids

54.67

Weight (kDa)

9.77

Isoelectric Point (pI)

47.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 482
AatII GACGTC 1 cut(s) 360
Acc16I TGCGCA 1 cut(s) 79
Acc36I ACCTGC 1 cut(s) 883
AccI GTMKAC 1 cut(s) 1118
AciI CCGC 4 cut(s) 70, 149, 921, 1357
AclWI GGATC 5 cut(s) 18, 317, 418, 1183, 1239
AcsI RAATTY 1 cut(s) 301
AcyI GRCGYC 2 cut(s) 357, 1033
AfaI GTAC 6 cut(s) 137, 396, 601, 1229, 1296, 1347
AfiI CCNNNNNNNGG 6 cut(s) 145, 174, 222, 258, 283, 944
AgsI TTSAA 8 cut(s) 50, 206, 967, 1153, 1215, 1234, 1364, 1410
AjnI CCWGG 2 cut(s) 643, 943
AluBI AGCT 5 cut(s) 228, 687, 740, 980, 1130
AluI AGCT 5 cut(s) 228, 687, 740, 980, 1130
Alw21I GWGCWC 1 cut(s) 1132
Alw26I GTCTC 4 cut(s) 242, 267, 348, 800
AlwI GGATC 5 cut(s) 18, 317, 418, 1183, 1239
AlwNI CAGNNNCTG 1 cut(s) 228
AoxI GGCC 4 cut(s) 20, 762, 1006, 1367
ApeKI GCWGC 3 cut(s) 672, 687, 980
ApoI RAATTY 1 cut(s) 301
ArsI GACNNNNNNTTYG 2 cut(s) 771, 803
Asp700I GAANNNNTTC 1 cut(s) 1305
AspLEI GCGC 3 cut(s) 36, 80, 826
AsuC2I CCSGG 2 cut(s) 614, 1060
AsuHPI GGTGA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 1132
BarI GAAGNNNNNNTAC 2 cut(s) 840, 872
BbsI GAAGAC 1 cut(s) 213
Bbv12I GWGCWC 1 cut(s) 1132
BbvI GCAGC 3 cut(s) 674, 684, 967
BccI CCATC 3 cut(s) 124, 274, 1153
BceAI ACGGC 1 cut(s) 162
BciT130I CCWGG 2 cut(s) 645, 945
BciVI GTATCC 1 cut(s) 586
BcnI CCSGG 2 cut(s) 614, 1060
BcoDI GTCTC 4 cut(s) 242, 267, 348, 800
BfaI CTAG 2 cut(s) 452, 1010
BfoI RGCGCY 2 cut(s) 37, 827
BfuAI ACCTGC 1 cut(s) 883
BfuI GTATCC 1 cut(s) 586
BisI GCNGC 3 cut(s) 673, 688, 981
BlsI GCNGC 3 cut(s) 674, 689, 982
BmcAI AGTACT 2 cut(s) 1229, 1296
Bme1390I CCNGG 4 cut(s) 614, 645, 945, 1060
BmiI GGNNCC 1 cut(s) 153
BmrFI CCNGG 4 cut(s) 614, 645, 945, 1060
BmrI ACTGGG 2 cut(s) 673, 874
BmsI GCATC 1 cut(s) 1295
BmuI ACTGGG 2 cut(s) 673, 874
BoxI GACNNNNGTC 1 cut(s) 254
BpiI GAAGAC 1 cut(s) 213
BplI GAGNNNNNCTC 4 cut(s) 143, 175, 589, 621
BpmI CTGGAG 1 cut(s) 726
BpuMI CCSGG 2 cut(s) 614, 1060
Bsa29I ATCGAT 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 711
BsaHI GRCGYC 2 cut(s) 357, 1033
BsaI GGTCTC 2 cut(s) 242, 267
BsaJI CCNNGG 3 cut(s) 17, 747, 1107
Bsc4I CCNNNNNNNGG 6 cut(s) 145, 174, 222, 258, 283, 944
Bse1I ACTGG 2 cut(s) 668, 880
Bse3DI GCAATG 3 cut(s) 62, 682, 708
Bse8I GATNNNNATC 1 cut(s) 711
BseBI CCWGG 2 cut(s) 645, 945
BseCI ATCGAT 1 cut(s) 422
BseDI CCNNGG 3 cut(s) 17, 747, 1107
BseGI GGATG 4 cut(s) 164, 352, 535, 622
BseJI GATNNNNATC 1 cut(s) 711
BseLI CCNNNNNNNGG 6 cut(s) 145, 174, 222, 258, 283, 944
BseMI GCAATG 3 cut(s) 62, 682, 708
BseMII CTCAG 1 cut(s) 1385
BseNI ACTGG 2 cut(s) 668, 880
BseXI GCAGC 3 cut(s) 674, 684, 967
BsgI GTGCAG 1 cut(s) 26
BshFI GGCC 4 cut(s) 22, 764, 1008, 1369
BshVI ATCGAT 1 cut(s) 422
BsiHKAI GWGCWC 1 cut(s) 1132
BsiSI CCGG 3 cut(s) 614, 1005, 1059
BslFI GGGAC 1 cut(s) 596
BslI CCNNNNNNNGG 6 cut(s) 145, 174, 222, 258, 283, 944
BsmAI GTCTC 4 cut(s) 242, 267, 348, 800
BsmBI CGTCTC 1 cut(s) 348
BsmFI GGGAC 1 cut(s) 596
BsmI GAATGC 1 cut(s) 62
BsnI GGCC 4 cut(s) 22, 764, 1008, 1369
Bso31I GGTCTC 2 cut(s) 242, 267
Bsp1286I GDGCHC 1 cut(s) 1132
Bsp1407I TGTACA 1 cut(s) 1345
Bsp143I GATC 7 cut(s) 10, 309, 419, 423, 853, 1188, 1244
Bsp19I CCATGG 2 cut(s) 747, 1107
BspACI CCGC 4 cut(s) 70, 149, 921, 1357
BspANI GGCC 4 cut(s) 22, 764, 1008, 1369
BspCNI CTCAG 1 cut(s) 1384
BspDI ATCGAT 1 cut(s) 422
BspHI TCATGA 1 cut(s) 654
BspLI GGNNCC 1 cut(s) 153
BspMI ACCTGC 1 cut(s) 883
BspPI GGATC 5 cut(s) 18, 317, 418, 1183, 1239
BspQI GCTCTTC 2 cut(s) 677, 970
BspTNI GGTCTC 2 cut(s) 242, 267
BsrDI GCAATG 3 cut(s) 62, 682, 708
BsrGI TGTACA 1 cut(s) 1345
BsrI ACTGG 2 cut(s) 668, 880
BssECI CCNNGG 3 cut(s) 17, 747, 1107
BssMI GATC 7 cut(s) 10, 309, 419, 423, 853, 1188, 1244
BssNI GRCGYC 2 cut(s) 357, 1033
BssT1I CCWWGG 3 cut(s) 17, 747, 1107
Bst2UI CCWGG 2 cut(s) 645, 945
Bst4CI ACNGT 4 cut(s) 27, 239, 628, 787
Bst6I CTCTTC 3 cut(s) 166, 677, 970
BstACI GRCGYC 2 cut(s) 357, 1033
BstAUI TGTACA 1 cut(s) 1345
BstC8I GCNNGC 1 cut(s) 1128
BstDEI CTNAG 3 cut(s) 512, 1198, 1371
BstDSI CCRYGG 2 cut(s) 747, 1107
BstEII GGTNACC 1 cut(s) 434
BstENI CCTNNNNNAGG 2 cut(s) 220, 942
BstF5I GGATG 4 cut(s) 164, 352, 535, 622
BstH2I RGCGCY 2 cut(s) 37, 827
BstHHI GCGC 3 cut(s) 36, 80, 826
BstKTI GATC 7 cut(s) 13, 312, 422, 426, 856, 1191, 1247
BstMAI GTCTC 4 cut(s) 242, 267, 348, 800
BstMBI GATC 7 cut(s) 10, 309, 419, 423, 853, 1188, 1244
BstMWI GCNNNNNNNGC 1 cut(s) 649
BstNI CCWGG 2 cut(s) 645, 945
BstPAI GACNNNNGTC 1 cut(s) 254
BstPI GGTNACC 1 cut(s) 434
BstSCI CCNGG 4 cut(s) 612, 643, 943, 1058
BstV1I GCAGC 3 cut(s) 674, 684, 967
BstV2I GAAGAC 1 cut(s) 213
BstX2I RGATCY 2 cut(s) 1188, 1244
BstYI RGATCY 2 cut(s) 1188, 1244
Bsu15I ATCGAT 1 cut(s) 422
BsuI GTATCC 1 cut(s) 586
BsuRI GGCC 4 cut(s) 22, 764, 1008, 1369
BsuTUI ATCGAT 1 cut(s) 422
BtgI CCRYGG 2 cut(s) 747, 1107
BtsCI GGATG 4 cut(s) 164, 352, 535, 622
BtsI GCAGTG 1 cut(s) 182
BtsIMutI CAGTG 2 cut(s) 23, 182
BveI ACCTGC 1 cut(s) 883
Cac8I GCNNGC 1 cut(s) 1128
CaiI CAGNNNCTG 1 cut(s) 228
CciI TCATGA 1 cut(s) 654
CfoI GCGC 3 cut(s) 36, 80, 826
ClaI ATCGAT 1 cut(s) 422
CseI GACGC 1 cut(s) 1041
Csp6I GTAC 6 cut(s) 136, 395, 600, 1228, 1295, 1346
CviAII CATG 7 cut(s) 75, 200, 655, 722, 748, 988, 1108
CviQI GTAC 6 cut(s) 136, 395, 600, 1228, 1295, 1346
DdeI CTNAG 3 cut(s) 512, 1198, 1371
DpnI GATC 7 cut(s) 12, 311, 421, 425, 855, 1190, 1246
DpnII GATC 7 cut(s) 10, 309, 419, 423, 853, 1188, 1244
DraI TTTAAA 1 cut(s) 1257
Eam1104I CTCTTC 3 cut(s) 166, 677, 970
EarI CTCTTC 3 cut(s) 166, 677, 970
EciI GGCGGA 2 cut(s) 59, 164
Ecl136II GAGCTC 1 cut(s) 1130
Eco130I CCWWGG 3 cut(s) 17, 747, 1107
Eco24I GRGCYC 1 cut(s) 1132
Eco31I GGTCTC 2 cut(s) 242, 267
Eco32I GATATC 2 cut(s) 523, 535
Eco53kI GAGCTC 1 cut(s) 1130
Eco91I GGTNACC 1 cut(s) 434
EcoICRI GAGCTC 1 cut(s) 1130
EcoNI CCTNNNNNAGG 2 cut(s) 220, 942
EcoO65I GGTNACC 1 cut(s) 434
EcoRII CCWGG 2 cut(s) 643, 943
EcoRV GATATC 2 cut(s) 523, 535
EcoT14I CCWWGG 3 cut(s) 17, 747, 1107
EcoT22I ATGCAT 1 cut(s) 901
EcoT38I GRGCYC 1 cut(s) 1132
ErhI CCWWGG 3 cut(s) 17, 747, 1107
Esp3I CGTCTC 1 cut(s) 348
FaeI CATG 7 cut(s) 78, 203, 658, 725, 751, 991, 1111
FalI AAGNNNNNCTT 4 cut(s) 674, 706, 967, 999
FaqI GGGAC 1 cut(s) 596
FatI CATG 7 cut(s) 74, 199, 654, 721, 747, 987, 1107
FblI GTMKAC 1 cut(s) 1118
Fnu4HI GCNGC 3 cut(s) 673, 688, 981
FokI GGATG 4 cut(s) 151, 359, 542, 629
FriOI GRGCYC 1 cut(s) 1132
Fsp4HI GCNGC 3 cut(s) 673, 688, 981
FspAI RTGCGCAY 1 cut(s) 79
FspBI CTAG 2 cut(s) 452, 1010
FspI TGCGCA 1 cut(s) 79
GlaI GCGC 3 cut(s) 35, 79, 825
GluI GCNGC 3 cut(s) 673, 688, 981
GsuI CTGGAG 1 cut(s) 726
HaeII RGCGCY 2 cut(s) 37, 827
HaeIII GGCC 4 cut(s) 22, 764, 1008, 1369
HapII CCGG 3 cut(s) 614, 1005, 1059
HgaI GACGC 1 cut(s) 1041
HhaI GCGC 3 cut(s) 36, 80, 826
Hin1I GRCGYC 2 cut(s) 357, 1033
Hin1II CATG 7 cut(s) 78, 203, 658, 725, 751, 991, 1111
Hin6I GCGC 3 cut(s) 34, 78, 824
HinP1I GCGC 3 cut(s) 34, 78, 824
HincII GTYRAC 1 cut(s) 1119
HindII GTYRAC 1 cut(s) 1119
HinfI GANTC 4 cut(s) 243, 887, 1098, 1301
HpaII CCGG 3 cut(s) 614, 1005, 1059
HphI GGTGA 1 cut(s) 5
Hpy166II GTNNAC 2 cut(s) 235, 1119
Hpy188I TCNGA 9 cut(s) 177, 196, 266, 886, 974, 1031, 1087, 1335, 1374
Hpy188III TCNNGA 4 cut(s) 247, 313, 595, 655
Hpy8I GTNNAC 2 cut(s) 235, 1119
Hpy99I CGWCG 2 cut(s) 124, 1123
HpyAV CCTTC 2 cut(s) 165, 261
HpyCH4III ACNGT 4 cut(s) 27, 239, 628, 787
HpyCH4IV ACGT 3 cut(s) 114, 357, 1204
HpyCH4V TGCA 6 cut(s) 7, 55, 187, 222, 899, 1076
HpyF10VI GCNNNNNNNGC 1 cut(s) 649
HpyF3I CTNAG 3 cut(s) 512, 1198, 1371
HpySE526I ACGT 3 cut(s) 114, 357, 1204
Hsp92I GRCGYC 2 cut(s) 357, 1033
Hsp92II CATG 7 cut(s) 78, 203, 658, 725, 751, 991, 1111
HspAI GCGC 3 cut(s) 34, 78, 824
Kzo9I GATC 7 cut(s) 10, 309, 419, 423, 853, 1188, 1244
LguI GCTCTTC 2 cut(s) 677, 970
LmnI GCTCC 4 cut(s) 151, 225, 745, 1135
Lsp1109I GCAGC 3 cut(s) 674, 684, 967
LweI GCATC 1 cut(s) 1295
MaeI CTAG 2 cut(s) 452, 1010
MaeII ACGT 3 cut(s) 114, 357, 1204
MaeIII GTNAC 3 cut(s) 434, 622, 888
MalI GATC 7 cut(s) 12, 311, 421, 425, 855, 1190, 1246
MboI GATC 7 cut(s) 10, 309, 419, 423, 853, 1188, 1244
MboII GAAGA 7 cut(s) 153, 218, 694, 848, 940, 987, 1037
MflI RGATCY 2 cut(s) 1188, 1244
MhlI GDGCHC 1 cut(s) 1132
MluCI AATT 8 cut(s) 301, 318, 477, 487, 727, 1090, 1235, 1273
MlyI GAGTC 1 cut(s) 896
MmeI TCCRAC 1 cut(s) 1313
Mph1103I ATGCAT 1 cut(s) 901
MroXI GAANNNNTTC 1 cut(s) 1305
MseI TTAA 6 cut(s) 291, 903, 1094, 1256, 1268, 1280
MslI CAYNNNNRTG 1 cut(s) 338
MspA1I CMGCKG 1 cut(s) 1357
MspI CCGG 3 cut(s) 614, 1005, 1059
MspR9I CCNGG 4 cut(s) 614, 645, 945, 1060
Mva1269I GAATGC 1 cut(s) 62
MvaI CCWGG 2 cut(s) 645, 945
MwoI GCNNNNNNNGC 1 cut(s) 649
NciI CCSGG 2 cut(s) 614, 1060
NcoI CCATGG 2 cut(s) 747, 1107
NdeII GATC 7 cut(s) 10, 309, 419, 423, 853, 1188, 1244
NlaIII CATG 7 cut(s) 78, 203, 658, 725, 751, 991, 1111
NlaIV GGNNCC 1 cut(s) 153
NmeAIII GCCGAG 1 cut(s) 740
NmuCI GTSAC 2 cut(s) 622, 888
NsbI TGCGCA 1 cut(s) 79
NsiI ATGCAT 1 cut(s) 901
PagI TCATGA 1 cut(s) 654
PciSI GCTCTTC 2 cut(s) 677, 970
PctI GAATGC 1 cut(s) 62
PdmI GAANNNNTTC 1 cut(s) 1305
PfeI GAWTC 3 cut(s) 243, 1098, 1301
PflFI GACNNNGTC 1 cut(s) 785
PfoI TCCNGGA 1 cut(s) 612
PkrI GCNGC 3 cut(s) 674, 689, 982
PleI GAGTC 1 cut(s) 895
PpsI GAGTC 1 cut(s) 895
PshAI GACNNNNGTC 1 cut(s) 254
PsiI TTATAA 1 cut(s) 482
Psp124BI GAGCTC 1 cut(s) 1132
Psp6I CCWGG 2 cut(s) 643, 943
PspEI GGTNACC 1 cut(s) 434
PspGI CCWGG 2 cut(s) 643, 943
PspN4I GGNNCC 1 cut(s) 153
PstNI CAGNNNCTG 1 cut(s) 228
PsuI RGATCY 2 cut(s) 1188, 1244
PsyI GACNNNGTC 1 cut(s) 785
RsaI GTAC 6 cut(s) 137, 396, 601, 1229, 1296, 1347
RsaNI GTAC 6 cut(s) 136, 395, 600, 1228, 1295, 1346
RseI CAYNNNNRTG 1 cut(s) 338
SacI GAGCTC 1 cut(s) 1132
SalI GTCGAC 1 cut(s) 1117
SapI GCTCTTC 2 cut(s) 677, 970
SaqAI TTAA 6 cut(s) 291, 903, 1094, 1256, 1268, 1280
SatI GCNGC 3 cut(s) 673, 688, 981
Sau3AI GATC 7 cut(s) 10, 309, 419, 423, 853, 1188, 1244
ScaI AGTACT 2 cut(s) 1229, 1296
SchI GAGTC 1 cut(s) 896
ScrFI CCNGG 4 cut(s) 614, 645, 945, 1060
SduI GDGCHC 1 cut(s) 1132
SfaNI GCATC 1 cut(s) 1295
SmiMI CAYNNNNRTG 1 cut(s) 338
Sse9I AATT 8 cut(s) 301, 318, 477, 487, 727, 1090, 1235, 1273
SsiI CCGC 4 cut(s) 70, 149, 921, 1357
SspI AATATT 2 cut(s) 405, 1156
SspMI CTAG 2 cut(s) 452, 1010
SstI GAGCTC 1 cut(s) 1132
StyD4I CCNGG 4 cut(s) 612, 643, 943, 1058
StyI CCWWGG 3 cut(s) 17, 747, 1107
TaaI ACNGT 4 cut(s) 27, 239, 628, 787
TaiI ACGT 3 cut(s) 117, 360, 1207
TaqI TCGA 7 cut(s) 119, 241, 246, 422, 754, 1118, 1299
TasI AATT 8 cut(s) 301, 318, 477, 487, 727, 1090, 1235, 1273
TatI WGTACW 4 cut(s) 599, 1227, 1294, 1345
TfiI GAWTC 3 cut(s) 243, 1098, 1301
Tru1I TTAA 6 cut(s) 291, 903, 1094, 1256, 1268, 1280
Tru9I TTAA 6 cut(s) 291, 903, 1094, 1256, 1268, 1280
TscAI CASTG 2 cut(s) 30, 189
TseFI GTSAC 2 cut(s) 622, 888
TseI GCWGC 3 cut(s) 672, 687, 980
Tsp45I GTSAC 2 cut(s) 622, 888
TspDTI ATGAA 5 cut(s) 125, 153, 633, 671, 834
TspGWI ACGGA 1 cut(s) 1163
TspRI CASTG 2 cut(s) 30, 189
Tth111I GACNNNGTC 1 cut(s) 785
XagI CCTNNNNNAGG 2 cut(s) 220, 942
XapI RAATTY 1 cut(s) 301
XmiI GTMKAC 1 cut(s) 1118
XmnI GAANNNNTTC 1 cut(s) 1305
XspI CTAG 2 cut(s) 452, 1010
ZraI GACGTC 1 cut(s) 358
ZrmI AGTACT 2 cut(s) 1229, 1296
Zsp2I ATGCAT 1 cut(s) 901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.