pycom01g03030

NYN domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0029187
Physical Location & Seq
Forward (+)
2442191 .. 2442586
396 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 396 bp
ATGAGACCCATTTCTCCAAAAACCCTCTACCGCCTCTCTGCCTCTTCGCCATCCGCTCCTCCTTTCCTCCTTCAGATTTCCCGCCTCTCAACCTCTTCGCTATCCCACCATTCGCACGCTCACTCTTCCTTCAGACGCCACGACGAGGAGGTTCGGAATGTCAGGGTTTCGGTTTGGTGGGATTTTGAGAACTGCAATTTGCCGGCCGGTGTAAATGTGTTCAAGGTAGCGAATGCAATCACTGCGGCGATCAGGGCCAATGGGATCAAAGGTCCTATCCAGATTACGGCTTTTGGGGATATGTTGCAGCTGTCAAGGTTCAACCAGGAGGCATTGTCGTCCACCGGGGTCAATCTCACCCACGTTCCTCACGGTTCGCTTCGTTCTTACATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NYN OHA OST-HTH

Protein Analysis

132

Amino Acids

14.48

Weight (kDa)

10.47

Isoelectric Point (pI)

47.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NYN PF01936 55 - 124 2.3e-09 NYN domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029187)

Species Orthologous Gene IDs
pyrus_communis pycom01g03030
rosa_multiflora Rmu_co8184576.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 56
AciI CCGC 4 cut(s) 31, 54, 82, 245
AclWI GGATC 1 cut(s) 272
AcoI YGGCCR 1 cut(s) 204
AcuI CTGAAG 2 cut(s) 56, 115
AcyI GRCGYC 1 cut(s) 136
AfiI CCNNNNNNNGG 2 cut(s) 145, 286
AgsI TTSAA 2 cut(s) 223, 322
AjnI CCWGG 1 cut(s) 324
AluBI AGCT 1 cut(s) 310
AluI AGCT 1 cut(s) 310
AlwI GGATC 1 cut(s) 272
AoxI GGCC 2 cut(s) 204, 255
ApeKI GCWGC 1 cut(s) 307
AspS9I GGNCC 2 cut(s) 255, 272
AsuC2I CCSGG 1 cut(s) 346
AsuHPI GGTGA 1 cut(s) 349
AvaII GGWCC 1 cut(s) 272
BbvI GCAGC 1 cut(s) 319
BccI CCATC 1 cut(s) 58
BceAI ACGGC 1 cut(s) 303
BciT130I CCWGG 1 cut(s) 326
BcnI CCSGG 1 cut(s) 346
BisI GCNGC 2 cut(s) 246, 308
BlsI GCNGC 2 cut(s) 247, 309
Bme1390I CCNGG 2 cut(s) 326, 346
Bme18I GGWCC 1 cut(s) 272
BmgT120I GGNCC 2 cut(s) 255, 272
BmrFI CCNGG 2 cut(s) 326, 346
BpuMI CCSGG 1 cut(s) 346
BsaHI GRCGYC 1 cut(s) 136
BsaJI CCNNGG 1 cut(s) 345
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 286
Bse118I RCCGGY 2 cut(s) 202, 206
BseBI CCWGG 1 cut(s) 326
BseDI CCNNGG 1 cut(s) 345
BseGI GGATG 1 cut(s) 50
BseLI CCNNNNNNNGG 2 cut(s) 145, 286
BseRI GAGGAG 2 cut(s) 48, 161
BseX3I CGGCCG 1 cut(s) 204
BseXI GCAGC 1 cut(s) 319
Bsh1285I CGRYCG 1 cut(s) 207
BshFI GGCC 2 cut(s) 206, 257
BsiEI CGRYCG 1 cut(s) 207
BsiSI CCGG 3 cut(s) 203, 207, 345
BslI CCNNNNNNNGG 2 cut(s) 145, 286
BsmI GAATGC 1 cut(s) 238
BsnI GGCC 2 cut(s) 206, 257
Bsp143I GATC 2 cut(s) 249, 264
BspACI CCGC 4 cut(s) 31, 54, 82, 245
BspANI GGCC 2 cut(s) 206, 257
BspPI GGATC 1 cut(s) 272
BsrBI CCGCTC 1 cut(s) 56
BsrFI RCCGGY 2 cut(s) 202, 206
BssAI RCCGGY 2 cut(s) 202, 206
BssECI CCNNGG 1 cut(s) 345
BssMI GATC 2 cut(s) 249, 264
BssNI GRCGYC 1 cut(s) 136
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 1 cut(s) 374
Bst6I CTCTTC 3 cut(s) 49, 100, 130
BstACI GRCGYC 1 cut(s) 136
BstAPI GCANNNNNTGC 1 cut(s) 242
BstC8I GCNNGC 2 cut(s) 117, 204
BstF5I GGATG 1 cut(s) 50
BstKTI GATC 2 cut(s) 252, 267
BstMBI GATC 2 cut(s) 249, 264
BstMCI CGRYCG 1 cut(s) 207
BstMWI GCNNNNNNNGC 2 cut(s) 242, 254
BstNI CCWGG 1 cut(s) 326
BstSCI CCNGG 2 cut(s) 324, 344
BstV1I GCAGC 1 cut(s) 319
BstZI CGGCCG 1 cut(s) 204
BsuRI GGCC 2 cut(s) 206, 257
BtsCI GGATG 1 cut(s) 50
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 1 cut(s) 240
Cac8I GCNNGC 2 cut(s) 117, 204
Cfr10I RCCGGY 2 cut(s) 202, 206
Cfr13I GGNCC 2 cut(s) 255, 272
CseI GACGC 1 cut(s) 144
CviJI RGCY 4 cut(s) 206, 257, 290, 310
CviKI_1 RGCY 4 cut(s) 206, 257, 290, 310
DpnI GATC 2 cut(s) 251, 266
DpnII GATC 2 cut(s) 249, 264
EaeI YGGCCR 1 cut(s) 204
EagI CGGCCG 1 cut(s) 204
Eam1104I CTCTTC 3 cut(s) 49, 100, 130
EarI CTCTTC 3 cut(s) 49, 100, 130
EclXI CGGCCG 1 cut(s) 204
Eco47I GGWCC 1 cut(s) 272
Eco52I CGGCCG 1 cut(s) 204
Eco57I CTGAAG 2 cut(s) 56, 115
EcoO109I RGGNCCY 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 324
FaiI YATR 1 cut(s) 302
FauI CCCGC 1 cut(s) 89
Fnu4HI GCNGC 2 cut(s) 246, 308
FokI GGATG 1 cut(s) 37
Fsp4HI GCNGC 2 cut(s) 246, 308
GluI GCNGC 2 cut(s) 246, 308
HaeIII GGCC 2 cut(s) 206, 257
HapII CCGG 3 cut(s) 203, 207, 345
HgaI GACGC 1 cut(s) 144
Hin1I GRCGYC 1 cut(s) 136
HpaII CCGG 3 cut(s) 203, 207, 345
HphI GGTGA 1 cut(s) 349
Hpy166II GTNNAC 1 cut(s) 342
Hpy188I TCNGA 3 cut(s) 75, 134, 156
Hpy188III TCNNGA 1 cut(s) 280
Hpy8I GTNNAC 1 cut(s) 342
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 2 cut(s) 80, 139
HpyCH4III ACNGT 1 cut(s) 374
HpyCH4IV ACGT 1 cut(s) 363
HpyCH4V TGCA 3 cut(s) 195, 236, 307
HpyF10VI GCNNNNNNNGC 2 cut(s) 242, 254
HpySE526I ACGT 1 cut(s) 363
Hsp92I GRCGYC 1 cut(s) 136
KroI GCCGGC 1 cut(s) 202
KroNI GCCGGC 1 cut(s) 204
Kzo9I GATC 2 cut(s) 249, 264
LmnI GCTCC 1 cut(s) 61
LpnPI CCDG 8 cut(s) 148, 216, 220, 238, 293, 311, 338, 358
Lsp1109I GCAGC 1 cut(s) 319
MaeII ACGT 1 cut(s) 363
MalI GATC 2 cut(s) 251, 266
MbiI CCGCTC 1 cut(s) 56
MboI GATC 2 cut(s) 249, 264
MboII GAAGA 3 cut(s) 36, 87, 117
MluCI AATT 1 cut(s) 196
MroNI GCCGGC 1 cut(s) 202
MseI TTAA 1 cut(s) 394
MspA1I CMGCKG 1 cut(s) 310
MspI CCGG 3 cut(s) 203, 207, 345
MspR9I CCNGG 2 cut(s) 326, 346
Mva1269I GAATGC 1 cut(s) 238
MvaI CCWGG 1 cut(s) 326
MwoI GCNNNNNNNGC 2 cut(s) 242, 254
NaeI GCCGGC 1 cut(s) 204
NciI CCSGG 1 cut(s) 346
NdeII GATC 2 cut(s) 249, 264
NgoMIV GCCGGC 1 cut(s) 202
PctI GAATGC 1 cut(s) 238
PdiI GCCGGC 1 cut(s) 204
PkrI GCNGC 2 cut(s) 247, 309
PpuMI RGGWCCY 1 cut(s) 272
Psp5II RGGWCCY 1 cut(s) 272
Psp6I CCWGG 1 cut(s) 324
PspGI CCWGG 1 cut(s) 324
PspPI GGNCC 2 cut(s) 255, 272
PspPPI RGGWCCY 1 cut(s) 272
PvuII CAGCTG 1 cut(s) 310
SaqAI TTAA 1 cut(s) 394
SatI GCNGC 2 cut(s) 246, 308
Sau3AI GATC 2 cut(s) 249, 264
Sau96I GGNCC 2 cut(s) 255, 272
ScrFI CCNGG 2 cut(s) 326, 346
SetI ASST 7 cut(s) 95, 153, 228, 274, 312, 320, 366
SinI GGWCC 1 cut(s) 272
Sse9I AATT 1 cut(s) 196
SsiI CCGC 4 cut(s) 31, 54, 82, 245
StyD4I CCNGG 2 cut(s) 324, 344
TaaI ACNGT 1 cut(s) 374
TaiI ACGT 1 cut(s) 366
TasI AATT 1 cut(s) 196
TauI GCSGC 1 cut(s) 248
Tru1I TTAA 1 cut(s) 394
Tru9I TTAA 1 cut(s) 394
TscAI CASTG 1 cut(s) 247
TseI GCWGC 1 cut(s) 307
TspRI CASTG 1 cut(s) 247
VpaK11BI GGWCC 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.