pycom01g03380

4,5-DOPA dioxygenase extradiol-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0029188
Physical Location & Seq
Forward (+)
2615505 .. 2617170
1666 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g03380.7

Sequence Viewer

Length: 1230 bp
ATGCATGAACATTGCATGAAAACTGGGTTCTTGGGTTTTCGAGGGTTTCAAGTTCTAAAAATGGTATGGATGTACTCAGGAGCTTGCAAGGAGTTCAAATCTTACCTTCTAGACGTCGATCCGTGCGGAAATGGAAGGAAATCCGAGAGAAAGAAGAGAGAAGGAGTCACGGGGAAAAAGGGATGGGTGTGTGTGGTCCAAATGGCAGCCCACCAACCAAAACAAATCAAACACCCTACAATGCTAATTAACAATTCGGTACGGGCTGTCATAGGACCTCTCGTTAATTTTAAACAACAAAAACCATTGCTTCTAATTAAACTTCTTGATCATTTAGCCAAATTTTATAAATTTATACTATGGCGTTTTGAACCTAAGACCTCTTTATTCATTTTAAACAACAAAACCACCAGTTCTAATCACCCTAATAATTTTGGTACTCCTAATTTTTTTGGACCCCGGATGATCGCCCTACTTACACTGGGCCTGGGCTGGGAGGGCGTCCTTATAGTGGGGTTAGGCAGTGCCACTCACAACTTAAGGGCGTTGAAGAGGGAGACAAAGAGCAATGCATGTGCTGTCCCTTGGGCTCTCGAGTTTGGCACGTGGATCAAGGAAGCTCTTCTGGAAGGAAAGTACGAGGATGTGAACGAGTATGTGGAGAAGGCGCCGCATGTGAAAATGGCGCACCCTTGGCCGGATCACTTCTACCCGTTGCATGTAGCAGTGGGGGCTGCTGGTCCGGATGCAAAGGCTGAGCAAATCCACGACAGCTGGGGGCTCGGTGCTCTTTCGTATGCCTCCTATCAGTTTACATCGCGCAGTGCATGTGTCTGTCGACGCTGCTCCCGCAGATCAAGCATGTCAATAAAACTGCTGTGGATGAATGATTTGTGGCTGAATCTTAATATCACTCAATGGCCCTTAGGATTTCTGCTTAATTTTATTGAGAAGAAACTAAAACTTTTGCTCCCTTTCAAAGGACATGAACACAGTATTCACCCAACAAATATCGTATCCTCAGTATTCACACAATGCTGTGGATGTTGTTTATTTATGCAATGGATTCAACCAACCCCATTACCTTTAAATTTATCTGGTCTGCACAAATTGCCTTTGTTAAACCTGAGGTTTGAACCTCCACTGGTATGTAAAGAATTGAGATACGGTAAGGATGTATTTGCCCTAGATTTTGATCAAGTACTCAACTGTTTTGGAAATAAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00965 map00965
Pfam Domains
Protein Families
LigB

Protein Analysis

410

Amino Acids

46.53

Weight (kDa)

9.41

Isoelectric Point (pI)

35.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0029188)

Species Orthologous Gene IDs
pyrus_communis pycom01g03380 pycom13g22960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 348
AatII GACGTC 1 cut(s) 117
AccB1I GGYRCC 1 cut(s) 667
AccI GTMKAC 1 cut(s) 838
AccII CGCG 1 cut(s) 820
AccIII TCCGGA 1 cut(s) 742
AciI CCGC 3 cut(s) 126, 671, 850
AclWI GGATC 3 cut(s) 113, 617, 708
AcoI YGGCCR 1 cut(s) 695
AcsI RAATTY 3 cut(s) 341, 350, 1090
AcvI CACGTG 1 cut(s) 606
AcyI GRCGYC 3 cut(s) 114, 501, 668
AfaI GTAC 5 cut(s) 74, 261, 439, 638, 1203
AfiI CCNNNNNNNGG 4 cut(s) 493, 511, 697, 980
AflII CTTAAG 1 cut(s) 538
AgsI TTSAA 7 cut(s) 50, 97, 371, 550, 979, 1070, 1136
AjnI CCWGG 1 cut(s) 486
AluBI AGCT 3 cut(s) 83, 620, 774
AluI AGCT 3 cut(s) 83, 620, 774
Alw21I GWGCWC 1 cut(s) 790
Alw26I GTCTC 1 cut(s) 551
AlwI GGATC 3 cut(s) 113, 617, 708
Ama87I CYCGRG 1 cut(s) 593
Aor13HI TCCGGA 1 cut(s) 742
AoxI GGCC 3 cut(s) 484, 695, 920
ApeKI GCWGC 3 cut(s) 206, 734, 843
ApoI RAATTY 3 cut(s) 341, 350, 1090
Asp700I GAANNNNTTC 1 cut(s) 621
AspLEI GCGC 3 cut(s) 670, 688, 822
AspS9I GGNCC 6 cut(s) 196, 275, 455, 484, 740, 921
AsuC2I CCSGG 1 cut(s) 460
AsuHPI GGTGA 2 cut(s) 413, 992
AvaI CYCGRG 1 cut(s) 593
AvaII GGWCC 4 cut(s) 196, 275, 455, 740
AxyI CCTNAGG 2 cut(s) 925, 1127
BanI GGYRCC 1 cut(s) 667
BanII GRGCYC 2 cut(s) 592, 783
BbrPI CACGTG 1 cut(s) 606
Bbv12I GWGCWC 1 cut(s) 790
BbvI GCAGC 3 cut(s) 218, 721, 830
BccI CCATC 1 cut(s) 177
BciT130I CCWGG 1 cut(s) 488
BciVI GTATCC 1 cut(s) 1027
BclI TGATCA 2 cut(s) 328, 1195
BcnI CCSGG 1 cut(s) 460
BcoDI GTCTC 1 cut(s) 551
BfaI CTAG 2 cut(s) 110, 1187
BfoI RGCGCY 1 cut(s) 671
BfrI CTTAAG 1 cut(s) 538
BfuI GTATCC 1 cut(s) 1027
BisI GCNGC 4 cut(s) 207, 671, 735, 844
BlpI GCTNAGC 1 cut(s) 756
BlsI GCNGC 4 cut(s) 208, 672, 736, 845
BmcAI AGTACT 1 cut(s) 1203
Bme1390I CCNGG 2 cut(s) 460, 488
Bme18I GGWCC 4 cut(s) 196, 275, 455, 740
BmeT110I CYCGRG 1 cut(s) 593
BmgT120I GGNCC 6 cut(s) 196, 275, 455, 484, 740, 921
BmiI GGNNCC 2 cut(s) 457, 669
BmrFI CCNGG 2 cut(s) 460, 488
BmrI ACTGGG 2 cut(s) 33, 491
BmsI GCATC 1 cut(s) 736
BmuI ACTGGG 2 cut(s) 33, 491
Bpu1102I GCTNAGC 1 cut(s) 756
BpuMI CCSGG 1 cut(s) 460
BsaAI YACGTR 1 cut(s) 606
BsaBI GATNNNNATC 1 cut(s) 1194
BsaHI GRCGYC 3 cut(s) 114, 501, 668
BsaJI CCNNGG 4 cut(s) 458, 487, 584, 692
BsaWI WCCGGW 1 cut(s) 742
BsaXI ACNNNNNCTCC 2 cut(s) 954, 984
Bsc4I CCNNNNNNNGG 4 cut(s) 493, 511, 697, 980
Bse1I ACTGG 4 cut(s) 28, 411, 486, 1149
Bse21I CCTNAGG 2 cut(s) 925, 1127
Bse3DI GCAATG 4 cut(s) 10, 305, 574, 1067
Bse8I GATNNNNATC 1 cut(s) 1194
BseAI TCCGGA 1 cut(s) 742
BseBI CCWGG 1 cut(s) 488
BseDI CCNNGG 4 cut(s) 458, 487, 584, 692
BseGI GGATG 8 cut(s) 75, 188, 468, 649, 751, 888, 1049, 1180
BseJI GATNNNNATC 1 cut(s) 1194
BseLI CCNNNNNNNGG 4 cut(s) 493, 511, 697, 980
BseMI GCAATG 4 cut(s) 10, 305, 574, 1067
BseMII CTCAG 4 cut(s) 90, 747, 1035, 1118
BseNI ACTGG 4 cut(s) 28, 411, 486, 1149
BseXI GCAGC 3 cut(s) 218, 721, 830
BseYI CCCAGC 2 cut(s) 492, 774
BsgI GTGCAG 1 cut(s) 1088
Bsh1236I CGCG 1 cut(s) 820
BshFI GGCC 3 cut(s) 486, 697, 922
BshNI GGYRCC 1 cut(s) 667
BsiHKAI GWGCWC 1 cut(s) 790
BsiHKCI CYCGRG 1 cut(s) 593
BsiSI CCGG 3 cut(s) 460, 698, 743
BslFI GGGAC 1 cut(s) 566
BslI CCNNNNNNNGG 4 cut(s) 493, 511, 697, 980
BsmAI GTCTC 1 cut(s) 551
BsmFI GGGAC 1 cut(s) 566
BsnI GGCC 3 cut(s) 486, 697, 922
BsoBI CYCGRG 1 cut(s) 593
Bsp1286I GDGCHC 3 cut(s) 592, 783, 790
Bsp13I TCCGGA 1 cut(s) 742
Bsp143I GATC 7 cut(s) 118, 328, 465, 609, 700, 854, 1195
Bsp1720I GCTNAGC 1 cut(s) 756
BspACI CCGC 3 cut(s) 126, 671, 850
BspANI GGCC 3 cut(s) 486, 697, 922
BspCNI CTCAG 4 cut(s) 89, 748, 1034, 1119
BspEI TCCGGA 1 cut(s) 742
BspFNI CGCG 1 cut(s) 820
BspLI GGNNCC 2 cut(s) 457, 669
BspPI GGATC 3 cut(s) 113, 617, 708
BspQI GCTCTTC 1 cut(s) 627
BspT107I GGYRCC 1 cut(s) 667
BspTI CTTAAG 1 cut(s) 538
BsrDI GCAATG 4 cut(s) 10, 305, 574, 1067
BsrI ACTGG 4 cut(s) 28, 411, 486, 1149
BssECI CCNNGG 4 cut(s) 458, 487, 584, 692
BssMI GATC 7 cut(s) 118, 328, 465, 609, 700, 854, 1195
BssNI GRCGYC 3 cut(s) 114, 501, 668
BssT1I CCWWGG 2 cut(s) 584, 692
Bst2UI CCWGG 1 cut(s) 488
Bst4CI ACNGT 3 cut(s) 995, 1169, 1211
Bst6I CTCTTC 3 cut(s) 149, 545, 627
BstACI GRCGYC 3 cut(s) 114, 501, 668
BstAFI CTTAAG 1 cut(s) 538
BstAPI GCANNNNNTGC 1 cut(s) 1111
BstBAI YACGTR 1 cut(s) 606
BstC8I GCNNGC 1 cut(s) 85
BstDEI CTNAG 6 cut(s) 76, 375, 756, 925, 1021, 1127
BstENI CCTNNNNNAGG 1 cut(s) 978
BstF5I GGATG 8 cut(s) 75, 188, 468, 649, 751, 888, 1049, 1180
BstFNI CGCG 1 cut(s) 820
BstH2I RGCGCY 1 cut(s) 671
BstHHI GCGC 3 cut(s) 670, 688, 822
BstKTI GATC 7 cut(s) 121, 331, 468, 612, 703, 857, 1198
BstMAI GTCTC 1 cut(s) 551
BstMBI GATC 7 cut(s) 118, 328, 465, 609, 700, 854, 1195
BstMWI GCNNNNNNNGC 6 cut(s) 498, 694, 731, 849, 858, 1111
BstNI CCWGG 1 cut(s) 488
BstNSI RCATGY 5 cut(s) 576, 677, 722, 831, 865
BstSCI CCNGG 2 cut(s) 458, 486
BstUI CGCG 1 cut(s) 820
BstV1I GCAGC 3 cut(s) 218, 721, 830
Bsu36I CCTNAGG 2 cut(s) 925, 1127
BsuI GTATCC 1 cut(s) 1027
BsuRI GGCC 3 cut(s) 486, 697, 922
BtgZI GCGATG 1 cut(s) 801
BtsCI GGATG 8 cut(s) 75, 188, 468, 649, 751, 888, 1049, 1180
BtsI GCAGTG 3 cut(s) 529, 732, 829
BtsIMutI CAGTG 5 cut(s) 479, 529, 732, 829, 1142
Cac8I GCNNGC 1 cut(s) 85
CfoI GCGC 3 cut(s) 670, 688, 822
Cfr13I GGNCC 6 cut(s) 196, 275, 455, 484, 740, 921
CseI GACGC 2 cut(s) 490, 849
Csp6I GTAC 5 cut(s) 73, 260, 438, 637, 1202
CviAII CATG 8 cut(s) 5, 16, 573, 674, 719, 828, 862, 986
CviQI GTAC 5 cut(s) 73, 260, 438, 637, 1202
DdeI CTNAG 6 cut(s) 76, 375, 756, 925, 1021, 1127
DinI GGCGCC 1 cut(s) 669
DpnI GATC 7 cut(s) 120, 330, 467, 611, 702, 856, 1197
DpnII GATC 7 cut(s) 118, 328, 465, 609, 700, 854, 1195
DraI TTTAAA 3 cut(s) 292, 396, 1089
EaeI YGGCCR 1 cut(s) 695
Eam1104I CTCTTC 3 cut(s) 149, 545, 627
EarI CTCTTC 3 cut(s) 149, 545, 627
Eco130I CCWWGG 2 cut(s) 584, 692
Eco24I GRGCYC 2 cut(s) 592, 783
Eco47I GGWCC 4 cut(s) 196, 275, 455, 740
Eco72I CACGTG 1 cut(s) 606
Eco81I CCTNAGG 2 cut(s) 925, 1127
Eco88I CYCGRG 1 cut(s) 593
EcoNI CCTNNNNNAGG 1 cut(s) 978
EcoO109I RGGNCCY 1 cut(s) 275
EcoRII CCWGG 1 cut(s) 486
EcoT14I CCWWGG 2 cut(s) 584, 692
EcoT22I ATGCAT 2 cut(s) 6, 574
EcoT38I GRGCYC 2 cut(s) 592, 783
EgeI GGCGCC 1 cut(s) 669
EheI GGCGCC 1 cut(s) 669
ErhI CCWWGG 2 cut(s) 584, 692
FaeI CATG 8 cut(s) 8, 19, 576, 677, 722, 831, 865, 989
FaqI GGGAC 1 cut(s) 566
FatI CATG 8 cut(s) 4, 15, 572, 673, 718, 827, 861, 985
FauI CCCGC 1 cut(s) 857
FbaI TGATCA 2 cut(s) 328, 1195
FblI GTMKAC 1 cut(s) 838
Fnu4HI GCNGC 4 cut(s) 207, 671, 735, 844
FokI GGATG 8 cut(s) 82, 195, 475, 656, 758, 895, 1056, 1187
FriOI GRGCYC 2 cut(s) 592, 783
Fsp4HI GCNGC 4 cut(s) 207, 671, 735, 844
FspBI CTAG 2 cut(s) 110, 1187
GlaI GCGC 3 cut(s) 669, 687, 821
GluI GCNGC 4 cut(s) 207, 671, 735, 844
GsaI CCCAGC 2 cut(s) 496, 778
HaeII RGCGCY 1 cut(s) 671
HaeIII GGCC 3 cut(s) 486, 697, 922
HapII CCGG 3 cut(s) 460, 698, 743
HgaI GACGC 2 cut(s) 490, 849
HhaI GCGC 3 cut(s) 670, 688, 822
Hin1I GRCGYC 3 cut(s) 114, 501, 668
Hin1II CATG 8 cut(s) 8, 19, 576, 677, 722, 831, 865, 989
Hin6I GCGC 3 cut(s) 668, 686, 820
HinP1I GCGC 3 cut(s) 668, 686, 820
HincII GTYRAC 1 cut(s) 839
HindII GTYRAC 1 cut(s) 839
HinfI GANTC 3 cut(s) 165, 901, 1066
HpaII CCGG 3 cut(s) 460, 698, 743
HphI GGTGA 2 cut(s) 413, 992
Hpy166II GTNNAC 3 cut(s) 649, 813, 839
Hpy188I TCNGA 1 cut(s) 145
Hpy188III TCNNGA 6 cut(s) 78, 110, 326, 593, 626, 743
Hpy8I GTNNAC 3 cut(s) 649, 813, 839
Hpy99I CGWCG 2 cut(s) 119, 843
HpyAV CCTTC 5 cut(s) 116, 129, 155, 623, 658
HpyCH4III ACNGT 3 cut(s) 995, 1169, 1211
HpyCH4IV ACGT 2 cut(s) 114, 605
HpyCH4V TGCA 9 cut(s) 4, 15, 87, 572, 718, 749, 827, 1060, 1105
HpyF10VI GCNNNNNNNGC 6 cut(s) 498, 694, 731, 849, 858, 1111
HpyF3I CTNAG 6 cut(s) 76, 375, 756, 925, 1021, 1127
HpySE526I ACGT 2 cut(s) 114, 605
Hsp92I GRCGYC 3 cut(s) 114, 501, 668
Hsp92II CATG 8 cut(s) 8, 19, 576, 677, 722, 831, 865, 989
HspAI GCGC 3 cut(s) 668, 686, 820
KasI GGCGCC 1 cut(s) 667
Kpn2I TCCGGA 1 cut(s) 742
Ksp22I TGATCA 2 cut(s) 328, 1195
Kzo9I GATC 7 cut(s) 118, 328, 465, 609, 700, 854, 1195
LguI GCTCTTC 1 cut(s) 627
LmnI GCTCC 3 cut(s) 80, 851, 975
Lsp1109I GCAGC 3 cut(s) 218, 721, 830
LweI GCATC 1 cut(s) 736
MaeI CTAG 2 cut(s) 110, 1187
MaeII ACGT 2 cut(s) 114, 605
MaeIII GTNAC 1 cut(s) 166
MalI GATC 7 cut(s) 120, 330, 467, 611, 702, 856, 1197
MboI GATC 7 cut(s) 118, 328, 465, 609, 700, 854, 1195
MboII GAAGA 4 cut(s) 166, 562, 614, 964
MhlI GDGCHC 3 cut(s) 592, 783, 790
Mly113I GGCGCC 1 cut(s) 668
MlyI GAGTC 1 cut(s) 174
Mph1103I ATGCAT 2 cut(s) 6, 574
MroI TCCGGA 1 cut(s) 742
MroXI GAANNNNTTC 1 cut(s) 621
MslI CAYNNNNRTG 1 cut(s) 1147
MspA1I CMGCKG 1 cut(s) 774
MspCI CTTAAG 1 cut(s) 538
MspI CCGG 3 cut(s) 460, 698, 743
MspR9I CCNGG 2 cut(s) 460, 488
MvaI CCWGG 1 cut(s) 488
MvnI CGCG 1 cut(s) 820
MwoI GCNNNNNNNGC 6 cut(s) 498, 694, 731, 849, 858, 1111
NarI GGCGCC 1 cut(s) 668
NciI CCSGG 1 cut(s) 460
NdeII GATC 7 cut(s) 118, 328, 465, 609, 700, 854, 1195
NlaIII CATG 8 cut(s) 8, 19, 576, 677, 722, 831, 865, 989
NlaIV GGNNCC 2 cut(s) 457, 669
NmuCI GTSAC 1 cut(s) 166
NsiI ATGCAT 2 cut(s) 6, 574
NspI RCATGY 5 cut(s) 576, 677, 722, 831, 865
PaeR7I CTCGAG 1 cut(s) 593
PciSI GCTCTTC 1 cut(s) 627
PdmI GAANNNNTTC 1 cut(s) 621
PfeI GAWTC 2 cut(s) 901, 1066
PkrI GCNGC 4 cut(s) 208, 672, 736, 845
PleI GAGTC 1 cut(s) 173
PluTI GGCGCC 1 cut(s) 671
PmaCI CACGTG 1 cut(s) 606
PmlI CACGTG 1 cut(s) 606
PpsI GAGTC 1 cut(s) 173
Ppu21I YACGTR 1 cut(s) 606
PpuMI RGGWCCY 1 cut(s) 275
PsiI TTATAA 1 cut(s) 348
Psp5II RGGWCCY 1 cut(s) 275
Psp6I CCWGG 1 cut(s) 486
PspCI CACGTG 1 cut(s) 606
PspFI CCCAGC 2 cut(s) 492, 774
PspGI CCWGG 1 cut(s) 486
PspN4I GGNNCC 2 cut(s) 457, 669
PspPI GGNCC 6 cut(s) 196, 275, 455, 484, 740, 921
PspPPI RGGWCCY 1 cut(s) 275
PvuII CAGCTG 1 cut(s) 774
RsaI GTAC 5 cut(s) 74, 261, 439, 638, 1203
RsaNI GTAC 5 cut(s) 73, 260, 438, 637, 1202
RseI CAYNNNNRTG 1 cut(s) 1147
SalI GTCGAC 1 cut(s) 837
SapI GCTCTTC 1 cut(s) 627
SatI GCNGC 4 cut(s) 207, 671, 735, 844
Sau3AI GATC 7 cut(s) 118, 328, 465, 609, 700, 854, 1195
Sau96I GGNCC 6 cut(s) 196, 275, 455, 484, 740, 921
ScaI AGTACT 1 cut(s) 1203
SchI GAGTC 1 cut(s) 174
ScrFI CCNGG 2 cut(s) 460, 488
SduI GDGCHC 3 cut(s) 592, 783, 790
SfaNI GCATC 1 cut(s) 736
SfoI GGCGCC 1 cut(s) 669
Sfr274I CTCGAG 1 cut(s) 593
SinI GGWCC 4 cut(s) 196, 275, 455, 740
SlaI CTCGAG 1 cut(s) 593
SmiMI CAYNNNNRTG 1 cut(s) 1147
SmlI CTYRAG 2 cut(s) 538, 593
SmoI CTYRAG 2 cut(s) 538, 593
SsiI CCGC 3 cut(s) 126, 671, 850
SspDI GGCGCC 1 cut(s) 667
SspMI CTAG 2 cut(s) 110, 1187
StyD4I CCNGG 2 cut(s) 458, 486
StyI CCWWGG 2 cut(s) 584, 692
TaaI ACNGT 3 cut(s) 995, 1169, 1211
TaiI ACGT 2 cut(s) 117, 608
TaqI TCGA 4 cut(s) 40, 117, 594, 838
TatI WGTACW 2 cut(s) 72, 1201
TauI GCSGC 1 cut(s) 673
TfiI GAWTC 2 cut(s) 901, 1066
TscAI CASTG 5 cut(s) 486, 529, 732, 829, 1149
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 3 cut(s) 206, 734, 843
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 5 cut(s) 21, 32, 379, 899, 1002
TspGWI ACGGA 1 cut(s) 111
TspRI CASTG 5 cut(s) 486, 529, 732, 829, 1149
Vha464I CTTAAG 1 cut(s) 538
VpaK11BI GGWCC 4 cut(s) 196, 275, 455, 740
XagI CCTNNNNNAGG 1 cut(s) 978
XapI RAATTY 3 cut(s) 341, 350, 1090
XbaI TCTAGA 1 cut(s) 109
XceI RCATGY 5 cut(s) 576, 677, 722, 831, 865
XhoI CTCGAG 1 cut(s) 593
XmiI GTMKAC 1 cut(s) 838
XmnI GAANNNNTTC 1 cut(s) 621
XspI CTAG 2 cut(s) 110, 1187
ZraI GACGTC 1 cut(s) 115
ZrmI AGTACT 1 cut(s) 1203
Zsp2I ATGCAT 2 cut(s) 6, 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.