pycom01g05160

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0029190
Physical Location & Seq
Forward (+)
4762106 .. 4765357
3252 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1638 bp
ATGGCAACCACAATGGGATGGAGGTATGCTGCCACCAATGGCAGCACCCTTTCCACTAATGATCTGGAAAGGAATGGTGATGCAAAAGTTCAAGATTCGGAGCCTCCAACACCACATTCTCTTTTAAAGATGGGTTCAAGAGATCGTAGTAGTAGCATGGAGGATACAGATGGGACATTGGCAAGTGTTGCTCAATGCATTGAGCAGCTGCGTCAAAGTTCCTCATCTGTACAAGACAAGGAGTATTCGTTGAAGCAGTTGCTTGAGCTTATCAATACTCGTGAAAATGCTTTCAGCGCTGTTGGATCTCACTCTCAAGCAGTTCCAGTGCTTGTTTCTCTTCTTCGATCAGGTTCAGTTGGAGTTAAGATACAAGCTGCTACTGTTTTAGGCTCTCTCTGCAAGGAGAATGAACTCAGGGTGAAAGTCTTGCTTGGAGGATGCATTCCGCCATTGCTTGGTCTACTCAGGTCCAGCTCAGCAGAAGGGCAAGTTGCATCTGCGAAGACAATTTATGCTGTTTCTCAAGGTGGTGCCAGGGATCATGTTGGATCAAAAATATTTTCAACTGAAGGAGTTGTGCCAGTGCTCTGGGAACAGCTACAAAAAGGGATCAAAACTGGTAGTTTGGTTGATAGTTTGTTGACTGGAGCGTTAAAGAACCTATCCAGCAGCACTGAGGGATTCTGGACTGCAACCTTCCAAGCTGGAGCAGTGGATATACTTGTGAAGTTGCTGACAACCGGGCAGTCAAGCACTCAAGCTAACGTGTGCTTTCTACTTGCATGTATGATGGTGGAAGATCCATCTGTCTGTTCTAAGGTGCTGGCTTCAGAGGCTACCAAACAACTTCTCAAGCTTTTAGGATCCGGAAATGAAGCTTCTGTTAGAGCAGAAGCTGCAGGTGCTTTTAAATCTCTTTCTGCCCAGTGCAAAGAAGCAAGACGGGAAATAGCTAACTTCAATGGCATTCCTGTATTGATAAATGCTACAATAGCCCCTTCCAAAGAATTCATGCAGGGTGAGTATGCCCAAGCGTTGCAGGAGAATGCGATGTGTGCTTTAGCAAATATTTCTGGTGGTTTGTCATATGTTATCTCAAGCCTTGGGCAAAGCCTTGAGTCTTGCACTTCACCTGCCCAAATTTCTGATACATTAGGGGCTTTGGCTTCAGCTTTGATGATATATGATAGTACAGCAGAATCTAATAGAGCATCAGATCCTGTGGTTATTGAGCAGACATTGGTTTCACAGTTCAAACCGCGTTTACCGTTTCTTGTTCAGGAACGAACCATAGAGGCCCTTGCGAGTTTGTATGGGAATTCTGTACTCTCAGTTAAACTTGCCAATTCAGAAGCAAAGCGTTTGCTTGTTGGTTTGATCACAATGGCAACAAATGAAGTTCAGGATGAGCTAATGAGAGCTCTTCTTGCACTTTGCAATAGTGAGGAAAGTCTGTGGTGTGCGCTTCAAGGCCGTGAAGGGGTTCAGCTGTTGATATCACTTCTTGGGCTTTCATCAGAACAGCAGCAAGAATGTGCAGTTGCACTGCTTTGCCTGCTTTCCAATGAGAATGACGAAAGTAAATGGGCTATTACTGCTGCTGGTGGCATACCTCCACTTGTTCAGATTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000271 GO:0000278 GO:0000281 GO:0000910 GO:0001101 GO:0002682 GO:0002683 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005794 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005911 GO:0005938 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007275 GO:0008017 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009414 GO:0009415 GO:0009506 GO:0009555 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009653 GO:0009664 GO:0009791 GO:0009832 GO:0009833 GO:0009856 GO:0009898 GO:0009900 GO:0009901 GO:0009908 GO:0009987 GO:0010035 GO:0010038 GO:0010208 GO:0010215 GO:0010330 GO:0010927 GO:0010941 GO:0012505 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0016049 GO:0016051 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0022414 GO:0022603 GO:0022607 GO:0030054 GO:0030198 GO:0030243 GO:0030244 GO:0030863 GO:0030865 GO:0030981 GO:0031122 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0032886 GO:0032989 GO:0032991 GO:0033036 GO:0033043 GO:0033365 GO:0033612 GO:0033692 GO:0034613 GO:0034637 GO:0034645 GO:0036211 GO:0036449 GO:0040007 GO:0042221 GO:0042546 GO:0042742 GO:0042981 GO:0043062 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043622 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044380 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0044706 GO:0045229 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048467 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048608 GO:0048609 GO:0048646 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0048868 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051179 GO:0051211 GO:0051239 GO:0051273 GO:0051274 GO:0051301 GO:0051493 GO:0051592 GO:0051641 GO:0051704 GO:0051707 GO:0055028 GO:0055044 GO:0060548 GO:0061458 GO:0061640 GO:0065007 GO:0070507 GO:0070647 GO:0070696 GO:0070726 GO:0070727 GO:0071554 GO:0071555 GO:0071668 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0072697 GO:0072698 GO:0072699 GO:0085029 GO:0090567 GO:0097435 GO:0098542 GO:0098552 GO:0098562 GO:0099080 GO:0099081 GO:0099402 GO:0099512 GO:0099513 GO:0099568 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1902494 GO:1903047 GO:1905421 GO:1990234 GO:1990752 GO:1990778 GO:2000026 GO:2000028 GO:2000067 GO:2000241 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm C2

Protein Analysis

546

Amino Acids

57.63

Weight (kDa)

5.08

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 63 - 313 1.4e-10 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0029190)

Species Orthologous Gene IDs
pyrus_communis pycom01g05160 pycom15g29610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 893, 1144
Acc36I ACCTGC 2 cut(s) 893, 1144
AccB1I GGYRCC 1 cut(s) 533
AccB7I CCANNNNNTGG 1 cut(s) 458
AccI GTMKAC 1 cut(s) 463
AccII CGCG 1 cut(s) 1264
AccIII TCCGGA 1 cut(s) 869
AciI CCGC 2 cut(s) 449, 1262
AclWI GGATC 8 cut(s) 313, 549, 559, 620, 797, 861, 874, 1214
AcsI RAATTY 3 cut(s) 1010, 1143, 1321
AcuI CTGAAG 3 cut(s) 591, 816, 1155
AfaI GTAC 3 cut(s) 231, 1195, 1329
AfeI AGCGCT 1 cut(s) 298
AfiI CCNNNNNNNGG 2 cut(s) 458, 1483
AflIII ACRYGT 1 cut(s) 768
AgsI TTSAA 7 cut(s) 92, 138, 253, 567, 964, 1258, 1472
AjnI CCWGG 1 cut(s) 536
AjuI GAANNNNNNNTTGG 2 cut(s) 100, 132
Alw21I GWGCWC 2 cut(s) 591, 1426
AlwI GGATC 8 cut(s) 313, 549, 559, 620, 797, 861, 874, 1214
AlwNI CAGNNNCTG 3 cut(s) 899, 1223, 1633
Aor13HI TCCGGA 1 cut(s) 869
Aor51HI AGCGCT 1 cut(s) 298
AoxI GGCC 2 cut(s) 1299, 1474
ApeKI GCWGC 9 cut(s) 29, 42, 205, 208, 377, 672, 899, 1528, 1601
ApoI RAATTY 3 cut(s) 1010, 1143, 1321
Asp700I GAANNNNTTC 1 cut(s) 1485
AspLEI GCGC 2 cut(s) 299, 1468
AspS9I GGNCC 2 cut(s) 471, 1300
AsuC2I CCSGG 1 cut(s) 745
AsuHPI GGTGA 4 cut(s) 89, 433, 1034, 1125
AvaII GGWCC 1 cut(s) 471
BamHI GGATCC 1 cut(s) 866
BanI GGYRCC 1 cut(s) 533
BanII GRGCYC 1 cut(s) 1426
BauI CACGAG 1 cut(s) 279
BbsI GAAGAC 1 cut(s) 512
Bbv12I GWGCWC 2 cut(s) 591, 1426
BbvI GCAGC 9 cut(s) 16, 54, 195, 217, 364, 684, 886, 1540, 1588
BccI CCATC 5 cut(s) 12, 124, 164, 787, 814
BceAI ACGGC 1 cut(s) 1461
BciT130I CCWGG 1 cut(s) 538
BciVI GTATCC 1 cut(s) 157
BclI TGATCA 1 cut(s) 1380
BcnI CCSGG 1 cut(s) 745
BfmI CTRYAG 2 cut(s) 900, 1634
BfoI RGCGCY 1 cut(s) 300
BfuAI ACCTGC 2 cut(s) 893, 1144
BfuI GTATCC 1 cut(s) 157
BisI GCNGC 9 cut(s) 30, 43, 206, 209, 378, 673, 900, 1529, 1602
BlpI GCTNAGC 1 cut(s) 478
BlsI GCNGC 9 cut(s) 31, 44, 207, 210, 379, 674, 901, 1530, 1603
Bme1390I CCNGG 2 cut(s) 538, 745
Bme18I GGWCC 1 cut(s) 471
BmgT120I GGNCC 2 cut(s) 471, 1300
BmiI GGNNCC 3 cut(s) 102, 535, 868
BmrFI CCNGG 2 cut(s) 538, 745
BmrI ACTGGG 1 cut(s) 922
BmsI GCATC 4 cut(s) 70, 431, 506, 1223
BmuI ACTGGG 1 cut(s) 922
BpiI GAAGAC 1 cut(s) 512
BpmI CTGGAG 2 cut(s) 669, 729
Bpu1102I GCTNAGC 1 cut(s) 478
BpuEI CTTGAG 7 cut(s) 284, 300, 510, 744, 839, 1084, 1139
BpuMI CCSGG 1 cut(s) 745
BsaJI CCNNGG 2 cut(s) 537, 1105
BsaWI WCCGGW 1 cut(s) 869
BsaXI ACNNNNNCTCC 2 cut(s) 233, 263
Bsc4I CCNNNNNNNGG 2 cut(s) 458, 1483
Bse1I ACTGG 5 cut(s) 326, 584, 625, 652, 928
Bse3DI GCAATG 1 cut(s) 452
BseAI TCCGGA 1 cut(s) 869
BseBI CCWGG 1 cut(s) 538
BseDI CCNNGG 2 cut(s) 537, 1105
BseGI GGATG 3 cut(s) 23, 446, 1414
BseLI CCNNNNNNNGG 2 cut(s) 458, 1483
BseMI GCAATG 1 cut(s) 452
BseMII CTCAG 5 cut(s) 430, 481, 492, 669, 1347
BseNI ACTGG 5 cut(s) 326, 584, 625, 652, 928
BseXI GCAGC 9 cut(s) 16, 54, 195, 217, 364, 684, 886, 1540, 1588
BsgI GTGCAG 1 cut(s) 1560
Bsh1236I CGCG 1 cut(s) 1264
BshFI GGCC 2 cut(s) 1301, 1476
BshNI GGYRCC 1 cut(s) 533
BsiHKAI GWGCWC 2 cut(s) 591, 1426
BsiSI CCGG 2 cut(s) 744, 870
BslFI GGGAC 1 cut(s) 187
BslI CCNNNNNNNGG 2 cut(s) 458, 1483
BsmFI GGGAC 1 cut(s) 187
BsmI GAATGC 3 cut(s) 444, 969, 1054
BsnI GGCC 2 cut(s) 1301, 1476
Bsp1286I GDGCHC 2 cut(s) 591, 1426
Bsp13I TCCGGA 1 cut(s) 869
Bsp1407I TGTACA 1 cut(s) 229
Bsp1720I GCTNAGC 1 cut(s) 478
BspACI CCGC 2 cut(s) 449, 1262
BspANI GGCC 2 cut(s) 1301, 1476
BspCNI CTCAG 5 cut(s) 429, 480, 491, 670, 1346
BspEI TCCGGA 1 cut(s) 869
BspFNI CGCG 1 cut(s) 1264
BspLI GGNNCC 3 cut(s) 102, 535, 868
BspMAI CTGCAG 1 cut(s) 904
BspMI ACCTGC 2 cut(s) 893, 1144
BspPI GGATC 8 cut(s) 313, 549, 559, 620, 797, 861, 874, 1214
BspQI GCTCTTC 1 cut(s) 1431
BspT107I GGYRCC 1 cut(s) 533
BsrDI GCAATG 1 cut(s) 452
BsrGI TGTACA 1 cut(s) 229
BsrI ACTGG 5 cut(s) 326, 584, 625, 652, 928
BssECI CCNNGG 2 cut(s) 537, 1105
BssSI CACGAG 1 cut(s) 279
BssT1I CCWWGG 1 cut(s) 1105
Bst2BI CACGAG 1 cut(s) 279
Bst2UI CCWGG 1 cut(s) 538
Bst4CI ACNGT 3 cut(s) 385, 1254, 1272
Bst6I CTCTTC 2 cut(s) 345, 1431
BstAPI GCANNNNNTGC 2 cut(s) 188, 899
BstAUI TGTACA 1 cut(s) 229
BstC8I GCNNGC 2 cut(s) 828, 1559
BstDEI CTNAG 6 cut(s) 416, 467, 478, 678, 819, 1333
BstF5I GGATG 3 cut(s) 23, 446, 1414
BstFNI CGCG 1 cut(s) 1264
BstH2I RGCGCY 1 cut(s) 300
BstHHI GCGC 2 cut(s) 299, 1468
BstNI CCWGG 1 cut(s) 538
BstNSI RCATGY 1 cut(s) 789
BstSCI CCNGG 2 cut(s) 536, 743
BstSFI CTRYAG 2 cut(s) 900, 1634
BstUI CGCG 1 cut(s) 1264
BstV1I GCAGC 9 cut(s) 16, 54, 195, 217, 364, 684, 886, 1540, 1588
BstV2I GAAGAC 1 cut(s) 512
BstX2I RGATCY 4 cut(s) 305, 802, 866, 1219
BstXI CCANNNNNNTGG 1 cut(s) 591
BstYI RGATCY 4 cut(s) 305, 802, 866, 1219
BsuI GTATCC 1 cut(s) 157
BsuRI GGCC 2 cut(s) 1301, 1476
BtgZI GCGATG 1 cut(s) 1067
BtsCI GGATG 3 cut(s) 23, 446, 1414
BtsI GCAGTG 2 cut(s) 720, 1547
BtsIMutI CAGTG 6 cut(s) 333, 591, 675, 720, 935, 1547
BveI ACCTGC 2 cut(s) 893, 1144
Cac8I GCNNGC 2 cut(s) 828, 1559
CaiI CAGNNNCTG 3 cut(s) 899, 1223, 1633
CfoI GCGC 2 cut(s) 299, 1468
Cfr13I GGNCC 2 cut(s) 471, 1300
CseI GACGC 1 cut(s) 200
Csp6I GTAC 3 cut(s) 230, 1194, 1328
CviAII CATG 4 cut(s) 157, 545, 786, 1015
CviQI GTAC 3 cut(s) 230, 1194, 1328
DdeI CTNAG 6 cut(s) 416, 467, 478, 678, 819, 1333
DraI TTTAAA 2 cut(s) 126, 913
Eam1104I CTCTTC 2 cut(s) 345, 1431
EarI CTCTTC 2 cut(s) 345, 1431
EciI GGCGGA 1 cut(s) 438
Ecl136II GAGCTC 1 cut(s) 1424
Eco130I CCWWGG 1 cut(s) 1105
Eco24I GRGCYC 1 cut(s) 1426
Eco32I GATATC 1 cut(s) 1500
Eco47I GGWCC 1 cut(s) 471
Eco47III AGCGCT 1 cut(s) 298
Eco53kI GAGCTC 1 cut(s) 1424
Eco57I CTGAAG 3 cut(s) 591, 816, 1155
EcoICRI GAGCTC 1 cut(s) 1424
EcoO109I RGGNCCY 1 cut(s) 1300
EcoRI GAATTC 2 cut(s) 1010, 1321
EcoRII CCWGG 1 cut(s) 536
EcoRV GATATC 1 cut(s) 1500
EcoT14I CCWWGG 1 cut(s) 1105
EcoT22I ATGCAT 2 cut(s) 200, 446
EcoT38I GRGCYC 1 cut(s) 1426
ErhI CCWWGG 1 cut(s) 1105
FaeI CATG 4 cut(s) 160, 548, 789, 1018
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FaqI GGGAC 1 cut(s) 187
FatI CATG 4 cut(s) 156, 544, 785, 1014
FauNDI CATATG 1 cut(s) 1090
FbaI TGATCA 1 cut(s) 1380
FblI GTMKAC 1 cut(s) 463
Fnu4HI GCNGC 9 cut(s) 30, 43, 206, 209, 378, 673, 900, 1529, 1602
FokI GGATG 3 cut(s) 30, 453, 1421
FriOI GRGCYC 1 cut(s) 1426
Fsp4HI GCNGC 9 cut(s) 30, 43, 206, 209, 378, 673, 900, 1529, 1602
GlaI GCGC 2 cut(s) 298, 1467
GluI GCNGC 9 cut(s) 30, 43, 206, 209, 378, 673, 900, 1529, 1602
GsuI CTGGAG 2 cut(s) 669, 729
HaeII RGCGCY 1 cut(s) 300
HaeIII GGCC 2 cut(s) 1301, 1476
HapII CCGG 2 cut(s) 744, 870
HgaI GACGC 1 cut(s) 200
HhaI GCGC 2 cut(s) 299, 1468
Hin1II CATG 4 cut(s) 160, 548, 789, 1018
Hin6I GCGC 2 cut(s) 297, 1466
HinP1I GCGC 2 cut(s) 297, 1466
HincII GTYRAC 1 cut(s) 645
HindII GTYRAC 1 cut(s) 645
HindIII AAGCTT 2 cut(s) 857, 879
HinfI GANTC 5 cut(s) 95, 684, 1121, 1202, 1630
HpaII CCGG 2 cut(s) 744, 870
HphI GGTGA 4 cut(s) 89, 433, 1034, 1125
Hpy166II GTNNAC 3 cut(s) 464, 645, 1268
Hpy188I TCNGA 7 cut(s) 100, 835, 1150, 1219, 1354, 1522, 1629
Hpy188III TCNNGA 8 cut(s) 65, 92, 138, 281, 688, 870, 1283, 1406
Hpy8I GTNNAC 3 cut(s) 464, 645, 1268
HpyAV CCTTC 5 cut(s) 479, 566, 709, 1011, 1475
HpyCH4III ACNGT 3 cut(s) 385, 1254, 1272
HpyCH4IV ACGT 1 cut(s) 768
HpyF3I CTNAG 6 cut(s) 416, 467, 478, 678, 819, 1333
HpySE526I ACGT 1 cut(s) 768
Hsp92II CATG 4 cut(s) 160, 548, 789, 1018
HspAI GCGC 2 cut(s) 297, 1466
Kpn2I TCCGGA 1 cut(s) 869
Ksp22I TGATCA 1 cut(s) 1380
LguI GCTCTTC 1 cut(s) 1431
LmnI GCTCC 3 cut(s) 100, 650, 710
Lsp1109I GCAGC 9 cut(s) 16, 54, 195, 217, 364, 684, 886, 1540, 1588
LweI GCATC 4 cut(s) 70, 431, 506, 1223
MaeII ACGT 1 cut(s) 768
MboII GAAGA 5 cut(s) 332, 335, 517, 812, 1418
MflI RGATCY 4 cut(s) 305, 802, 866, 1219
MhlI GDGCHC 2 cut(s) 591, 1426
MluCI AATT 5 cut(s) 510, 1010, 1143, 1321, 1348
MlyI GAGTC 1 cut(s) 1130
MmeI TCCRAC 4 cut(s) 131, 283, 340, 529
Mph1103I ATGCAT 2 cut(s) 200, 446
MroI TCCGGA 1 cut(s) 869
MroXI GAANNNNTTC 1 cut(s) 1485
MseI TTAA 5 cut(s) 125, 366, 656, 912, 1338
MspA1I CMGCKG 2 cut(s) 208, 1492
MspI CCGG 2 cut(s) 744, 870
MspR9I CCNGG 2 cut(s) 538, 745
Mva1269I GAATGC 3 cut(s) 444, 969, 1054
MvaI CCWGG 1 cut(s) 538
MvnI CGCG 1 cut(s) 1264
NciI CCSGG 1 cut(s) 745
NdeI CATATG 1 cut(s) 1090
NlaIII CATG 4 cut(s) 160, 548, 789, 1018
NlaIV GGNNCC 3 cut(s) 102, 535, 868
NsiI ATGCAT 2 cut(s) 200, 446
NspI RCATGY 1 cut(s) 789
PaqCI CACCTGC 2 cut(s) 893, 1144
PciSI GCTCTTC 1 cut(s) 1431
PctI GAATGC 3 cut(s) 444, 969, 1054
PdmI GAANNNNTTC 1 cut(s) 1485
PfeI GAWTC 4 cut(s) 95, 684, 1202, 1630
PflMI CCANNNNNTGG 1 cut(s) 458
PkrI GCNGC 9 cut(s) 31, 44, 207, 210, 379, 674, 901, 1530, 1603
PleI GAGTC 1 cut(s) 1129
PpsI GAGTC 1 cut(s) 1129
Psp124BI GAGCTC 1 cut(s) 1426
Psp6I CCWGG 1 cut(s) 536
PspGI CCWGG 1 cut(s) 536
PspN4I GGNNCC 3 cut(s) 102, 535, 868
PspPI GGNCC 2 cut(s) 471, 1300
PstI CTGCAG 1 cut(s) 904
PstNI CAGNNNCTG 3 cut(s) 899, 1223, 1633
PsuI RGATCY 4 cut(s) 305, 802, 866, 1219
PvuII CAGCTG 2 cut(s) 208, 1492
RsaI GTAC 3 cut(s) 231, 1195, 1329
RsaNI GTAC 3 cut(s) 230, 1194, 1328
SacI GAGCTC 1 cut(s) 1426
SapI GCTCTTC 1 cut(s) 1431
SaqAI TTAA 5 cut(s) 125, 366, 656, 912, 1338
SatI GCNGC 9 cut(s) 30, 43, 206, 209, 378, 673, 900, 1529, 1602
Sau96I GGNCC 2 cut(s) 471, 1300
SchI GAGTC 1 cut(s) 1130
ScrFI CCNGG 2 cut(s) 538, 745
SduI GDGCHC 2 cut(s) 591, 1426
SfaNI GCATC 4 cut(s) 70, 431, 506, 1223
SfcI CTRYAG 2 cut(s) 900, 1634
SinI GGWCC 1 cut(s) 471
SmlI CTYRAG 7 cut(s) 263, 315, 525, 759, 854, 1099, 1118
SmoI CTYRAG 7 cut(s) 263, 315, 525, 759, 854, 1099, 1118
Sse9I AATT 5 cut(s) 510, 1010, 1143, 1321, 1348
SsiI CCGC 2 cut(s) 449, 1262
SspI AATATT 2 cut(s) 561, 1072
SstI GAGCTC 1 cut(s) 1426
StyD4I CCNGG 2 cut(s) 536, 743
StyI CCWWGG 1 cut(s) 1105
TaaI ACNGT 3 cut(s) 385, 1254, 1272
TaiI ACGT 1 cut(s) 771
TaqI TCGA 1 cut(s) 346
TasI AATT 5 cut(s) 510, 1010, 1143, 1321, 1348
TatI WGTACW 3 cut(s) 229, 1193, 1327
TfiI GAWTC 4 cut(s) 95, 684, 1202, 1630
Tru1I TTAA 5 cut(s) 125, 366, 656, 912, 1338
Tru9I TTAA 5 cut(s) 125, 366, 656, 912, 1338
TscAI CASTG 6 cut(s) 333, 591, 682, 720, 935, 1554
TseI GCWGC 9 cut(s) 29, 42, 205, 208, 377, 672, 899, 1528, 1601
TspDTI ATGAA 5 cut(s) 426, 891, 1003, 1413, 1506
TspRI CASTG 6 cut(s) 333, 591, 682, 720, 935, 1554
Van91I CCANNNNNTGG 1 cut(s) 458
VpaK11BI GGWCC 1 cut(s) 471
XapI RAATTY 3 cut(s) 1010, 1143, 1321
XceI RCATGY 1 cut(s) 789
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
XmiI GTMKAC 1 cut(s) 463
XmnI GAANNNNTTC 1 cut(s) 1485
Zsp2I ATGCAT 2 cut(s) 200, 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.