pycom01g13010

modification-dependent protein catabolic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0024380
Physical Location & Seq
Reverse (-)
13596690 .. 13597127
438 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 438 bp
ATGTTGTCGGTCTCAAAGAGTAGGGTTGTGAAACTGAGGAGCTCAAACAACGAAATATTCGAAGTAGAAGAAGCTATGGCAGCGCTGTCGGAACCCATAAAATCCCTAGTCGACGTGATCGGTGTCAACCATTTGATACCATTGCCAGAGGTCAACTCCAAAACCCTAGTCATGGTGATAGAGTGGTGGAAGATTAAGCACAGCAATGCTTCTCATCAGGTTACTACAAAAAGCACGTCCAACACACAACTAATCAAAGAGTGGGAGGCTGAGTTTGCAGAGGAACTGGACCAAGCCAACGTTGTCATTGATCTCTTATGCACTGCGAACTTTTTATCCGTGAAGGAATTGCAAGATCTTATGTTTCAAAAGCTTGCAGATTTGATTAAGGTCAAGACAGTTGAGGAGATTTGCGAGTTGTTTCCCAGCAAGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04114 ko04120 ko04141 ko04310 ko04341 ko04350 ko04710 ko05168 ko05200 map04110 map04111 map04114 map04120 map04141 map04310 map04341 map04350 map04710 map05168 map05200
Pfam Domains
Protein Families
Skp1 Skp1_POZ

Protein Analysis

146

Amino Acids

16.42

Weight (kDa)

5.23

Isoelectric Point (pI)

31.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Skp1_POZ PF03931 9 - 66 3.3e-12 Skp1 family, tetramerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024380)

Species Orthologous Gene IDs
malus_domestica MD01G1103600.v1.1
prunus_persica Prupe.2G209400_v2.0.a1
pyrus_communis pycom01g13010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 111
AclI AACGTT 1 cut(s) 300
AfeI AGCGCT 1 cut(s) 84
AfiI CCNNNNNNNGG 1 cut(s) 172
AgsI TTSAA 1 cut(s) 368
AjiI CACGTC 2 cut(s) 115, 237
AloI GAACNNNNNNTCC 2 cut(s) 320, 352
AluBI AGCT 3 cut(s) 42, 74, 373
AluI AGCT 3 cut(s) 42, 74, 373
Alw21I GWGCWC 1 cut(s) 44
Alw26I GTCTC 1 cut(s) 16
Aor51HI AGCGCT 1 cut(s) 84
ApeKI GCWGC 1 cut(s) 80
ArsI GACNNNNNNTTYG 4 cut(s) 153, 185, 221, 253
AspLEI GCGC 1 cut(s) 85
AspS9I GGNCC 1 cut(s) 289
AsuHPI GGTGA 1 cut(s) 187
AsuII TTCGAA 1 cut(s) 60
AvaII GGWCC 1 cut(s) 289
BanII GRGCYC 1 cut(s) 44
Bbv12I GWGCWC 1 cut(s) 44
BbvI GCAGC 1 cut(s) 92
BcgI CGANNNNNNTGC 2 cut(s) 69, 103
BcoDI GTCTC 1 cut(s) 16
BfaI CTAG 2 cut(s) 107, 167
BfoI RGCGCY 1 cut(s) 86
BglII AGATCT 1 cut(s) 355
BisI GCNGC 1 cut(s) 81
BlsI GCNGC 1 cut(s) 82
Bme18I GGWCC 1 cut(s) 289
BmgBI CACGTC 2 cut(s) 115, 237
BmgT120I GGNCC 1 cut(s) 289
BmiI GGNNCC 1 cut(s) 93
BplI GAGNNNNNCTC 2 cut(s) 140, 172
Bpu14I TTCGAA 1 cut(s) 60
BsaI GGTCTC 1 cut(s) 16
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 1 cut(s) 172
Bse1I ACTGG 1 cut(s) 291
Bse3DI GCAATG 2 cut(s) 140, 211
BseLI CCNNNNNNNGG 1 cut(s) 172
BseMI GCAATG 2 cut(s) 140, 211
BseMII CTCAG 2 cut(s) 26, 261
BseNI ACTGG 1 cut(s) 291
BseRI GAGGAG 2 cut(s) 52, 419
BseXI GCAGC 1 cut(s) 92
BseYI CCCAGC 1 cut(s) 425
BsiHKAI GWGCWC 1 cut(s) 44
BslI CCNNNNNNNGG 1 cut(s) 172
BsmAI GTCTC 1 cut(s) 16
Bso31I GGTCTC 1 cut(s) 16
Bsp119I TTCGAA 1 cut(s) 60
Bsp1286I GDGCHC 1 cut(s) 44
Bsp143I GATC 3 cut(s) 117, 310, 355
BspCNI CTCAG 2 cut(s) 27, 262
BspLI GGNNCC 1 cut(s) 93
BspT104I TTCGAA 1 cut(s) 60
BspTNI GGTCTC 1 cut(s) 16
BsrDI GCAATG 2 cut(s) 140, 211
BsrI ACTGG 1 cut(s) 291
BssMI GATC 3 cut(s) 117, 310, 355
Bst4CI ACNGT 1 cut(s) 400
BstBI TTCGAA 1 cut(s) 60
BstC8I GCNNGC 2 cut(s) 375, 431
BstDEI CTNAG 2 cut(s) 35, 270
BstH2I RGCGCY 1 cut(s) 86
BstHHI GCGC 1 cut(s) 85
BstKTI GATC 3 cut(s) 120, 313, 358
BstMAI GTCTC 1 cut(s) 16
BstMBI GATC 3 cut(s) 117, 310, 355
BstMWI GCNNNNNNNGC 2 cut(s) 80, 275
BstV1I GCAGC 1 cut(s) 92
BstX2I RGATCY 1 cut(s) 355
BstYI RGATCY 1 cut(s) 355
BtrI CACGTC 2 cut(s) 115, 237
BtsI GCAGTG 1 cut(s) 321
BtsIMutI CAGTG 1 cut(s) 321
Cac8I GCNNGC 2 cut(s) 375, 431
CfoI GCGC 1 cut(s) 85
Cfr13I GGNCC 1 cut(s) 289
CviAII CATG 1 cut(s) 172
CviJI RGCY 5 cut(s) 42, 74, 269, 296, 373
CviKI_1 RGCY 5 cut(s) 42, 74, 269, 296, 373
DdeI CTNAG 2 cut(s) 35, 270
DpnI GATC 3 cut(s) 119, 312, 357
DpnII GATC 3 cut(s) 117, 310, 355
Ecl136II GAGCTC 1 cut(s) 42
Eco24I GRGCYC 1 cut(s) 44
Eco31I GGTCTC 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 289
Eco47III AGCGCT 1 cut(s) 84
Eco53kI GAGCTC 1 cut(s) 42
EcoICRI GAGCTC 1 cut(s) 42
EcoT38I GRGCYC 1 cut(s) 44
FaeI CATG 1 cut(s) 175
FaiI YATR 5 cut(s) 77, 98, 173, 319, 362
FatI CATG 1 cut(s) 171
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 1 cut(s) 81
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 1 cut(s) 81
FspBI CTAG 2 cut(s) 107, 167
GlaI GCGC 1 cut(s) 84
GluI GCNGC 1 cut(s) 81
GsaI CCCAGC 1 cut(s) 429
HaeII RGCGCY 1 cut(s) 86
HhaI GCGC 1 cut(s) 85
Hin1II CATG 1 cut(s) 175
Hin6I GCGC 1 cut(s) 83
HinP1I GCGC 1 cut(s) 83
HincII GTYRAC 3 cut(s) 112, 127, 154
HindII GTYRAC 3 cut(s) 112, 127, 154
HindIII AAGCTT 1 cut(s) 371
HphI GGTGA 1 cut(s) 187
Hpy166II GTNNAC 3 cut(s) 112, 127, 154
Hpy188I TCNGA 1 cut(s) 91
Hpy188III TCNNGA 1 cut(s) 394
Hpy8I GTNNAC 3 cut(s) 112, 127, 154
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 1 cut(s) 337
HpyCH4III ACNGT 1 cut(s) 400
HpyCH4IV ACGT 3 cut(s) 114, 236, 300
HpyCH4V TGCA 4 cut(s) 278, 321, 352, 377
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 275
HpyF3I CTNAG 2 cut(s) 35, 270
HpySE526I ACGT 3 cut(s) 114, 236, 300
Hsp92II CATG 1 cut(s) 175
HspAI GCGC 1 cut(s) 83
Kzo9I GATC 3 cut(s) 117, 310, 355
LmnI GCTCC 1 cut(s) 39
LpnPI CCDG 3 cut(s) 159, 203, 272
Lsp1109I GCAGC 1 cut(s) 92
MaeI CTAG 2 cut(s) 107, 167
MaeII ACGT 3 cut(s) 114, 236, 300
MaeIII GTNAC 1 cut(s) 220
MalI GATC 3 cut(s) 119, 312, 357
MboI GATC 3 cut(s) 117, 310, 355
MboII GAAGA 2 cut(s) 80, 202
MflI RGATCY 1 cut(s) 355
MhlI GDGCHC 1 cut(s) 44
MluCI AATT 1 cut(s) 347
MmeI TCCRAC 2 cut(s) 69, 264
MnlI CCTC 5 cut(s) 30, 142, 259, 274, 397
MseI TTAA 3 cut(s) 195, 387, 436
MslI CAYNNNNRTG 1 cut(s) 204
MwoI GCNNNNNNNGC 2 cut(s) 80, 275
NdeII GATC 3 cut(s) 117, 310, 355
NlaIII CATG 1 cut(s) 175
NlaIV GGNNCC 1 cut(s) 93
NspV TTCGAA 1 cut(s) 60
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PkrI GCNGC 1 cut(s) 82
Psp124BI GAGCTC 1 cut(s) 44
Psp1406I AACGTT 1 cut(s) 300
PspFI CCCAGC 1 cut(s) 425
PspN4I GGNNCC 1 cut(s) 93
PspPI GGNCC 1 cut(s) 289
PsuI RGATCY 1 cut(s) 355
RseI CAYNNNNRTG 1 cut(s) 204
SacI GAGCTC 1 cut(s) 44
SalI GTCGAC 1 cut(s) 110
SaqAI TTAA 3 cut(s) 195, 387, 436
SatI GCNGC 1 cut(s) 81
Sau3AI GATC 3 cut(s) 117, 310, 355
Sau96I GGNCC 1 cut(s) 289
SduI GDGCHC 1 cut(s) 44
SetI ASST 9 cut(s) 44, 76, 117, 153, 222, 239, 303, 375, 393
SfuI TTCGAA 1 cut(s) 60
SinI GGWCC 1 cut(s) 289
SmiMI CAYNNNNRTG 1 cut(s) 204
Sse9I AATT 1 cut(s) 347
SspI AATATT 1 cut(s) 57
SspMI CTAG 2 cut(s) 107, 167
SstI GAGCTC 1 cut(s) 44
TaaI ACNGT 1 cut(s) 400
TaiI ACGT 3 cut(s) 117, 239, 303
TaqI TCGA 2 cut(s) 60, 111
TasI AATT 1 cut(s) 347
Tru1I TTAA 3 cut(s) 195, 387, 436
Tru9I TTAA 3 cut(s) 195, 387, 436
TscAI CASTG 1 cut(s) 328
TseI GCWGC 1 cut(s) 80
TspGWI ACGGA 1 cut(s) 328
TspRI CASTG 1 cut(s) 328
VpaK11BI GGWCC 1 cut(s) 289
XmiI GTMKAC 1 cut(s) 111
XspI CTAG 2 cut(s) 107, 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.