pycom01g19850

aminotransferase ACS12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0024384
Physical Location & Seq
Reverse (-)
18409670 .. 18410358
689 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 492 bp
ATGTGCTCTCTATACTGCCTCCAGCTCTACCTAAAACGACGCCGACCCTCCAAACCCGATCCACCTTCCCCCTCCCAGTCCTCCTCCAACCTGGCGGAGCTCCCCAGAACGCTGTCGCGGTCCAGCTTGTCCACCCGCGGGTCCATGGGCCGGGTCCGGATGTCTTCCCAGGCGAGTTGGGTTGCGAAGCCCAATGACTCGCCGTACTATATAGGGTTGGATAGGGCTTCGGAGGACCCGTACGATAGGGTGGATAATCCGGATGGAGTTATTCAATTCGGATTGTCCGAGAACAGGCTGTGTTTGGATTTGATTGAGAAATGGGTTTCGGAGAATTTGATGGAATCGATGGTGGGAACGGACGGTGGTGATTTGAGCATAAGTGGGATTGCAGAGTACCAGCCGTTTGATGGAATGACTGAACTGAAAGTGGATAAGTGTTATTTACACACCTATTTTTCCAATGGCCAAAAAATTAAGAGGAGTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

164

Amino Acids

18.28

Weight (kDa)

6.58

Isoelectric Point (pI)

60.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024384)

Species Orthologous Gene IDs
malus_domestica MD01G1186700.v1.1 MD01G1186900.v1.1
pyrus_communis pycom01g19850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 118, 138
AccIII TCCGGA 2 cut(s) 156, 259
AciI CCGC 4 cut(s) 95, 118, 136, 138
AclWI GGATC 1 cut(s) 53
AcoI YGGCCR 1 cut(s) 466
AcsI RAATTY 1 cut(s) 334
AcyI GRCGYC 1 cut(s) 40
AfaI GTAC 3 cut(s) 206, 242, 398
AfiI CCNNNNNNNGG 4 cut(s) 138, 150, 294, 410
AgsI TTSAA 1 cut(s) 275
AjnI CCWGG 2 cut(s) 90, 168
AluBI AGCT 3 cut(s) 25, 100, 126
AluI AGCT 3 cut(s) 25, 100, 126
Alw21I GWGCWC 2 cut(s) 8, 102
AlwI GGATC 1 cut(s) 53
Aor13HI TCCGGA 2 cut(s) 156, 259
AoxI GGCC 2 cut(s) 148, 466
ApoI RAATTY 1 cut(s) 334
AspS9I GGNCC 5 cut(s) 120, 141, 148, 154, 235
AsuC2I CCSGG 1 cut(s) 152
AsuHPI GGTGA 1 cut(s) 380
AvaII GGWCC 4 cut(s) 120, 141, 154, 235
BalI TGGCCA 1 cut(s) 468
BanII GRGCYC 1 cut(s) 102
BbsI GAAGAC 1 cut(s) 156
Bbv12I GWGCWC 2 cut(s) 8, 102
BccI CCATC 4 cut(s) 257, 334, 343, 404
BceAI ACGGC 2 cut(s) 187, 388
BciT130I CCWGG 2 cut(s) 92, 170
BcnI CCSGG 1 cut(s) 152
Bme1390I CCNGG 3 cut(s) 92, 152, 170
Bme18I GGWCC 4 cut(s) 120, 141, 154, 235
BmgT120I GGNCC 5 cut(s) 120, 141, 148, 154, 235
BmiI GGNNCC 3 cut(s) 142, 155, 237
BmrFI CCNGG 3 cut(s) 92, 152, 170
BmrI ACTGGG 1 cut(s) 70
BmuI ACTGGG 1 cut(s) 70
BpiI GAAGAC 1 cut(s) 156
BpmI CTGGAG 1 cut(s) 5
BpuMI CCSGG 1 cut(s) 152
Bsa29I ATCGAT 1 cut(s) 347
BsaHI GRCGYC 1 cut(s) 40
BsaJI CCNNGG 3 cut(s) 136, 144, 168
BsaWI WCCGGW 2 cut(s) 156, 259
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bsc4I CCNNNNNNNGG 4 cut(s) 138, 150, 294, 410
Bse1I ACTGG 1 cut(s) 76
BseAI TCCGGA 2 cut(s) 156, 259
BseBI CCWGG 2 cut(s) 92, 170
BseCI ATCGAT 1 cut(s) 347
BseDI CCNNGG 3 cut(s) 136, 144, 168
BseGI GGATG 2 cut(s) 165, 268
BseLI CCNNNNNNNGG 4 cut(s) 138, 150, 294, 410
BseNI ACTGG 1 cut(s) 76
BseRI GAGGAG 1 cut(s) 73
Bsh1236I CGCG 2 cut(s) 118, 138
BshFI GGCC 2 cut(s) 150, 468
BshVI ATCGAT 1 cut(s) 347
BsiHKAI GWGCWC 2 cut(s) 8, 102
BsiSI CCGG 3 cut(s) 151, 157, 260
BsiWI CGTACG 1 cut(s) 240
BslI CCNNNNNNNGG 4 cut(s) 138, 150, 294, 410
BsnI GGCC 2 cut(s) 150, 468
Bsp1286I GDGCHC 2 cut(s) 8, 102
Bsp13I TCCGGA 2 cut(s) 156, 259
Bsp143I GATC 1 cut(s) 58
Bsp19I CCATGG 1 cut(s) 144
BspACI CCGC 4 cut(s) 95, 118, 136, 138
BspANI GGCC 2 cut(s) 150, 468
BspDI ATCGAT 1 cut(s) 347
BspEI TCCGGA 2 cut(s) 156, 259
BspFNI CGCG 2 cut(s) 118, 138
BspLI GGNNCC 3 cut(s) 142, 155, 237
BspPI GGATC 1 cut(s) 53
BsrI ACTGG 1 cut(s) 76
BssECI CCNNGG 3 cut(s) 136, 144, 168
BssMI GATC 1 cut(s) 58
BssNI GRCGYC 1 cut(s) 40
BssT1I CCWWGG 1 cut(s) 144
Bst2UI CCWGG 2 cut(s) 92, 170
Bst4CI ACNGT 1 cut(s) 365
BstACI GRCGYC 1 cut(s) 40
BstDSI CCRYGG 2 cut(s) 136, 144
BstF5I GGATG 2 cut(s) 165, 268
BstFNI CGCG 2 cut(s) 118, 138
BstKTI GATC 1 cut(s) 61
BstMBI GATC 1 cut(s) 58
BstNI CCWGG 2 cut(s) 92, 170
BstSCI CCNGG 3 cut(s) 90, 150, 168
BstUI CGCG 2 cut(s) 118, 138
BstV2I GAAGAC 1 cut(s) 156
Bsu15I ATCGAT 1 cut(s) 347
BsuRI GGCC 2 cut(s) 150, 468
BsuTUI ATCGAT 1 cut(s) 347
BtgI CCRYGG 2 cut(s) 136, 144
BtsCI GGATG 2 cut(s) 165, 268
Cfr13I GGNCC 5 cut(s) 120, 141, 148, 154, 235
Cfr42I CCGCGG 1 cut(s) 139
ClaI ATCGAT 1 cut(s) 347
CseI GACGC 1 cut(s) 48
Csp6I GTAC 3 cut(s) 205, 241, 397
CviAII CATG 1 cut(s) 145
CviJI RGCY 9 cut(s) 25, 100, 126, 150, 190, 227, 298, 403, 468
CviKI_1 RGCY 9 cut(s) 25, 100, 126, 150, 190, 227, 298, 403, 468
CviQI GTAC 3 cut(s) 205, 241, 397
DpnI GATC 1 cut(s) 60
DpnII GATC 1 cut(s) 58
EaeI YGGCCR 1 cut(s) 466
EciI GGCGGA 1 cut(s) 110
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 144
Eco24I GRGCYC 1 cut(s) 102
Eco47I GGWCC 4 cut(s) 120, 141, 154, 235
Eco53kI GAGCTC 1 cut(s) 100
EcoICRI GAGCTC 1 cut(s) 100
EcoO109I RGGNCCY 1 cut(s) 235
EcoRII CCWGG 2 cut(s) 90, 168
EcoT14I CCWWGG 1 cut(s) 144
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 1 cut(s) 148
FaiI YATR 5 cut(s) 13, 146, 210, 212, 380
FatI CATG 1 cut(s) 144
FauI CCCGC 2 cut(s) 131, 143
FokI GGATG 2 cut(s) 172, 275
FriOI GRGCYC 1 cut(s) 102
GsuI CTGGAG 1 cut(s) 5
HaeIII GGCC 2 cut(s) 150, 468
HapII CCGG 3 cut(s) 151, 157, 260
HgaI GACGC 1 cut(s) 48
Hin1I GRCGYC 1 cut(s) 40
Hin1II CATG 1 cut(s) 148
HinfI GANTC 2 cut(s) 197, 344
HpaII CCGG 3 cut(s) 151, 157, 260
HphI GGTGA 1 cut(s) 380
Hpy166II GTNNAC 1 cut(s) 132
Hpy188I TCNGA 4 cut(s) 232, 281, 289, 331
Hpy188III TCNNGA 2 cut(s) 157, 260
Hpy8I GTNNAC 1 cut(s) 132
Hpy99I CGWCG 1 cut(s) 42
HpyAV CCTTC 1 cut(s) 75
HpyCH4III ACNGT 1 cut(s) 365
HpyCH4V TGCA 1 cut(s) 392
Hsp92I GRCGYC 1 cut(s) 40
Hsp92II CATG 1 cut(s) 148
Kpn2I TCCGGA 2 cut(s) 156, 259
KspI CCGCGG 1 cut(s) 139
Kzo9I GATC 1 cut(s) 58
LmnI GCTCC 2 cut(s) 97, 105
MalI GATC 1 cut(s) 60
MboI GATC 1 cut(s) 58
MboII GAAGA 1 cut(s) 156
MhlI GDGCHC 2 cut(s) 8, 102
MlsI TGGCCA 1 cut(s) 468
MluCI AATT 3 cut(s) 275, 334, 474
MluNI TGGCCA 1 cut(s) 468
MlyI GAGTC 1 cut(s) 191
MmeI TCCRAC 2 cut(s) 111, 198
MnlI CCTC 7 cut(s) 29, 58, 82, 91, 94, 226, 474
Mox20I TGGCCA 1 cut(s) 468
MroI TCCGGA 2 cut(s) 156, 259
MscI TGGCCA 1 cut(s) 468
MseI TTAA 1 cut(s) 477
Msp20I TGGCCA 1 cut(s) 468
MspA1I CMGCKG 1 cut(s) 138
MspI CCGG 3 cut(s) 151, 157, 260
MspR9I CCNGG 3 cut(s) 92, 152, 170
MvaI CCWGG 2 cut(s) 92, 170
MvnI CGCG 2 cut(s) 118, 138
NciI CCSGG 1 cut(s) 152
NcoI CCATGG 1 cut(s) 144
NdeII GATC 1 cut(s) 58
NlaIII CATG 1 cut(s) 148
NlaIV GGNNCC 3 cut(s) 142, 155, 237
PcsI WCGNNNNNNNCGW 2 cut(s) 236, 285
PfeI GAWTC 1 cut(s) 344
Pfl23II CGTACG 1 cut(s) 240
PleI GAGTC 1 cut(s) 191
PpsI GAGTC 1 cut(s) 191
PpuMI RGGWCCY 1 cut(s) 235
Psp124BI GAGCTC 1 cut(s) 102
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 2 cut(s) 90, 168
PspGI CCWGG 2 cut(s) 90, 168
PspLI CGTACG 1 cut(s) 240
PspN4I GGNNCC 3 cut(s) 142, 155, 237
PspPI GGNCC 5 cut(s) 120, 141, 148, 154, 235
PspPPI RGGWCCY 1 cut(s) 235
RsaI GTAC 3 cut(s) 206, 242, 398
RsaNI GTAC 3 cut(s) 205, 241, 397
SacI GAGCTC 1 cut(s) 102
SacII CCGCGG 1 cut(s) 139
SaqAI TTAA 1 cut(s) 477
Sau3AI GATC 1 cut(s) 58
Sau96I GGNCC 5 cut(s) 120, 141, 148, 154, 235
SchI GAGTC 1 cut(s) 191
ScrFI CCNGG 3 cut(s) 92, 152, 170
SduI GDGCHC 2 cut(s) 8, 102
SetI ASST 7 cut(s) 27, 33, 67, 93, 102, 128, 455
Sfr303I CCGCGG 1 cut(s) 139
SgrBI CCGCGG 1 cut(s) 139
SinI GGWCC 4 cut(s) 120, 141, 154, 235
Sse9I AATT 3 cut(s) 275, 334, 474
SsiI CCGC 4 cut(s) 95, 118, 136, 138
SstI GAGCTC 1 cut(s) 102
StyD4I CCNGG 3 cut(s) 90, 150, 168
StyI CCWWGG 1 cut(s) 144
TaaI ACNGT 1 cut(s) 365
TaqI TCGA 1 cut(s) 347
TasI AATT 3 cut(s) 275, 334, 474
TfiI GAWTC 1 cut(s) 344
Tru1I TTAA 1 cut(s) 477
Tru9I TTAA 1 cut(s) 477
TspGWI ACGGA 1 cut(s) 374
VpaK11BI GGWCC 4 cut(s) 120, 141, 154, 235
XapI RAATTY 1 cut(s) 334
XcmI CCANNNNNNNNNTGG 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.