pycom01g22380

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0029206
Physical Location & Seq
Forward (+)
20001903 .. 20002755
853 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g22380.1

Sequence Viewer

Length: 426 bp
ATGTCGCAGGTGGTGGCTATGCGGGCGATTCAGAGCTCGATCCTCTCTCCCACCTCTGAATCCGCACACCGCCGCGCCGCCTTCAAGCTGAAGCCATCGACGAGCTTTGCATACAAAGTTATGGCCTTTGAGGAGAAGCAGAGGGGATGGAGCTGGAGACTGTTTGGGAACAGGAGGTCTGAGATCACTGCGAAGCGTCCCCTTCAGACGGAGGTCGCTCCCGTCTCACCCGAAGACTCACCCAAGATAGAGGAGCAGTGGAAGGCAATTCAGCAACTTGATGACCCAGCAGTGGGTATGTGGTCCAAGCCTGTTGTCAAACGTAAGACAAAGATTGTTTGCACAATTGGTCCTTCCACCAACACACGCGAAATGATCTGTAAGTTGGCCGAGGCTGGAATGAATGTTGCTCGTATGAATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006631 GO:0006633 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

142

Amino Acids

15.91

Weight (kDa)

10.07

Isoelectric Point (pI)

72.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 108 - 140 1.7e-10 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029206)

Species Orthologous Gene IDs
pyrus_communis pycom01g22380 pycom07g25690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 75, 369
AciI CCGC 5 cut(s) 22, 63, 70, 73, 78
AclWI GGATC 1 cut(s) 34
AcoI YGGCCR 1 cut(s) 387
AcuI CTGAAG 2 cut(s) 110, 188
AfiI CCNNNNNNNGG 3 cut(s) 208, 292, 293
AgsI TTSAA 1 cut(s) 85
AluBI AGCT 4 cut(s) 36, 88, 105, 153
AluI AGCT 4 cut(s) 36, 88, 105, 153
Alw21I GWGCWC 1 cut(s) 38
Alw26I GTCTC 2 cut(s) 151, 229
AlwI GGATC 1 cut(s) 34
AoxI GGCC 2 cut(s) 123, 387
AspLEI GCGC 1 cut(s) 77
AspS9I GGNCC 2 cut(s) 303, 350
AsuHPI GGTGA 2 cut(s) 219, 231
AvaII GGWCC 2 cut(s) 303, 350
BanII GRGCYC 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 38
BccI CCATC 2 cut(s) 103, 141
BcoDI GTCTC 2 cut(s) 151, 229
BisI GCNGC 2 cut(s) 73, 78
BlsI GCNGC 2 cut(s) 74, 79
Bme18I GGWCC 2 cut(s) 303, 350
BmgT120I GGNCC 2 cut(s) 303, 350
BoxI GACNNNNGTC 1 cut(s) 212
BpiI GAAGAC 1 cut(s) 240
BpmI CTGGAG 1 cut(s) 175
BsaJI CCNNGG 1 cut(s) 390
Bsc4I CCNNNNNNNGG 3 cut(s) 208, 292, 293
BseDI CCNNGG 1 cut(s) 390
BseGI GGATG 1 cut(s) 152
BseLI CCNNNNNNNGG 3 cut(s) 208, 292, 293
BseMII CTCAG 1 cut(s) 171
BseRI GAGGAG 2 cut(s) 146, 266
BseYI CCCAGC 1 cut(s) 286
Bsh1236I CGCG 2 cut(s) 75, 369
BshFI GGCC 2 cut(s) 125, 389
BsiHKAI GWGCWC 1 cut(s) 38
BslFI GGGAC 1 cut(s) 183
BslI CCNNNNNNNGG 3 cut(s) 208, 292, 293
BsmAI GTCTC 2 cut(s) 151, 229
BsmBI CGTCTC 1 cut(s) 229
BsmFI GGGAC 1 cut(s) 183
BsnI GGCC 2 cut(s) 125, 389
Bsp1286I GDGCHC 1 cut(s) 38
Bsp143I GATC 3 cut(s) 39, 183, 375
BspACI CCGC 5 cut(s) 22, 63, 70, 73, 78
BspANI GGCC 2 cut(s) 125, 389
BspCNI CTCAG 1 cut(s) 172
BspFNI CGCG 2 cut(s) 75, 369
BspPI GGATC 1 cut(s) 34
BssECI CCNNGG 1 cut(s) 390
BssMI GATC 3 cut(s) 39, 183, 375
Bst4CI ACNGT 1 cut(s) 162
BstC8I GCNNGC 1 cut(s) 24
BstDEI CTNAG 1 cut(s) 180
BstF5I GGATG 1 cut(s) 152
BstFNI CGCG 2 cut(s) 75, 369
BstHHI GCGC 1 cut(s) 77
BstKTI GATC 3 cut(s) 42, 186, 378
BstMAI GTCTC 2 cut(s) 151, 229
BstMBI GATC 3 cut(s) 39, 183, 375
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstPAI GACNNNNGTC 1 cut(s) 212
BstUI CGCG 2 cut(s) 75, 369
BstV2I GAAGAC 1 cut(s) 240
BsuRI GGCC 2 cut(s) 125, 389
BtsCI GGATG 1 cut(s) 152
BtsI GCAGTG 3 cut(s) 186, 263, 297
BtsIMutI CAGTG 3 cut(s) 186, 263, 297
Cac8I GCNNGC 1 cut(s) 24
CfoI GCGC 1 cut(s) 77
Cfr13I GGNCC 2 cut(s) 303, 350
CseI GACGC 1 cut(s) 185
DdeI CTNAG 1 cut(s) 180
DpnI GATC 3 cut(s) 41, 185, 377
DpnII GATC 3 cut(s) 39, 183, 375
EaeI YGGCCR 1 cut(s) 387
Ecl136II GAGCTC 1 cut(s) 36
Eco24I GRGCYC 1 cut(s) 38
Eco47I GGWCC 2 cut(s) 303, 350
Eco53kI GAGCTC 1 cut(s) 36
Eco57I CTGAAG 2 cut(s) 110, 188
EcoICRI GAGCTC 1 cut(s) 36
EcoT38I GRGCYC 1 cut(s) 38
Esp3I CGTCTC 1 cut(s) 229
FaiI YATR 6 cut(s) 20, 112, 122, 299, 416, 422
FaqI GGGAC 1 cut(s) 183
FauI CCCGC 1 cut(s) 15
Fnu4HI GCNGC 2 cut(s) 73, 78
FokI GGATG 1 cut(s) 159
FriOI GRGCYC 1 cut(s) 38
Fsp4HI GCNGC 2 cut(s) 73, 78
GlaI GCGC 1 cut(s) 76
GluI GCNGC 2 cut(s) 73, 78
GsaI CCCAGC 1 cut(s) 290
GsuI CTGGAG 1 cut(s) 175
HaeIII GGCC 2 cut(s) 125, 389
HgaI GACGC 1 cut(s) 185
HhaI GCGC 1 cut(s) 77
Hin6I GCGC 1 cut(s) 75
HinP1I GCGC 1 cut(s) 75
HinfI GANTC 3 cut(s) 28, 59, 236
HphI GGTGA 2 cut(s) 219, 231
Hpy188I TCNGA 4 cut(s) 33, 58, 181, 207
Hpy99I CGWCG 1 cut(s) 103
HpyAV CCTTC 4 cut(s) 91, 212, 256, 363
HpyCH4III ACNGT 1 cut(s) 162
HpyCH4IV ACGT 1 cut(s) 322
HpyCH4V TGCA 2 cut(s) 110, 342
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpyF3I CTNAG 1 cut(s) 180
HpySE526I ACGT 1 cut(s) 322
HspAI GCGC 1 cut(s) 75
Kzo9I GATC 3 cut(s) 39, 183, 375
LmnI GCTCC 3 cut(s) 150, 223, 253
LpnPI CCDG 5 cut(s) 139, 157, 300, 324, 381
MaeII ACGT 1 cut(s) 322
MalI GATC 3 cut(s) 41, 185, 377
MboI GATC 3 cut(s) 39, 183, 375
MboII GAAGA 1 cut(s) 245
MfeI CAATTG 1 cut(s) 345
MhlI GDGCHC 1 cut(s) 38
MluCI AATT 2 cut(s) 267, 345
MlyI GAGTC 1 cut(s) 230
MnlI CCTC 8 cut(s) 53, 64, 124, 135, 168, 205, 244, 385
MunI CAATTG 1 cut(s) 345
MvnI CGCG 2 cut(s) 75, 369
MwoI GCNNNNNNNGC 1 cut(s) 23
NdeII GATC 3 cut(s) 39, 183, 375
NmeAIII GCCGAG 1 cut(s) 415
PfeI GAWTC 2 cut(s) 28, 59
PkrI GCNGC 2 cut(s) 74, 79
PleI GAGTC 1 cut(s) 230
PpsI GAGTC 1 cut(s) 230
PshAI GACNNNNGTC 1 cut(s) 212
Psp124BI GAGCTC 1 cut(s) 38
PspFI CCCAGC 1 cut(s) 286
PspPI GGNCC 2 cut(s) 303, 350
SacI GAGCTC 1 cut(s) 38
SatI GCNGC 2 cut(s) 73, 78
Sau3AI GATC 3 cut(s) 39, 183, 375
Sau96I GGNCC 2 cut(s) 303, 350
SchI GAGTC 1 cut(s) 230
SduI GDGCHC 1 cut(s) 38
SetI ASST 9 cut(s) 12, 38, 56, 90, 107, 155, 179, 216, 325
SinI GGWCC 2 cut(s) 303, 350
Sse9I AATT 2 cut(s) 267, 345
SsiI CCGC 5 cut(s) 22, 63, 70, 73, 78
SstI GAGCTC 1 cut(s) 38
TaaI ACNGT 1 cut(s) 162
TaiI ACGT 1 cut(s) 325
TaqI TCGA 2 cut(s) 38, 98
TasI AATT 2 cut(s) 267, 345
TauI GCSGC 2 cut(s) 75, 80
TfiI GAWTC 2 cut(s) 28, 59
TscAI CASTG 3 cut(s) 193, 263, 297
TspDTI ATGAA 1 cut(s) 416
TspGWI ACGGA 1 cut(s) 224
TspRI CASTG 3 cut(s) 193, 263, 297
VpaK11BI GGWCC 2 cut(s) 303, 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.