pycom01g24170

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0028616
Physical Location & Seq
Forward (+)
21182741 .. 21183598
858 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 528 bp
ATGCTGAAATGTTCTGTTACTTTTATTTTCTACCCGCAGCGTTCTCAGGTTCATTTTGACTCGACTACTGCATGTGAATACCCCGACACTCCGGCTTTTGGACTTGGTGTAACTGCTGCAGTGGCTCTTATGTTAGCTCAAATAATTATAAATGTTTCAACCGGGTGCAATTGTTGCCGAAGGAGGCCCCAGCCTTCAAACTCTAACTCGACGGTAGCACAGTTACTCATTGTTTTTTCCTGGTTCGCATTCGTTATAGCGTTTTTTCTGCTGCTCGCTGGTGCCGCACTCAATCACCGGCATGGTGTAGAAATCACATACTCTGGCAGCTACGACTGTTACGTTGTGAAACCCGGAGTGTTTGCTGGAGGTGCCCTTTTGTCATTTGCTAGTGTGGTACTCGGAATTGTCTACTATGTCCACTTAAACTCAGCAAAGAACAGTGATATGTCAGGGGGCGGTTCTATCCCTAATCGAGGGGCAGTAGCTATGGGGCAACCTCGGATCCCGCCAACCCAATGTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

176

Amino Acids

18.63

Weight (kDa)

8.74

Isoelectric Point (pI)

35.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 39 - 132 3.5e-28 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028616)

Species Orthologous Gene IDs
malus_domestica MD01G1233400.v1.1
pyrus_communis pycom01g24170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 149
AccB1I GGYRCC 2 cut(s) 281, 371
AccI GTMKAC 1 cut(s) 411
AciI CCGC 4 cut(s) 35, 285, 459, 509
AclWI GGATC 2 cut(s) 499, 512
AfaI GTAC 1 cut(s) 399
AfiI CCNNNNNNNGG 2 cut(s) 98, 476
AgsI TTSAA 2 cut(s) 159, 198
AjnI CCWGG 1 cut(s) 239
AluBI AGCT 3 cut(s) 137, 330, 488
AluI AGCT 3 cut(s) 137, 330, 488
AlwI GGATC 2 cut(s) 499, 512
AoxI GGCC 1 cut(s) 185
ApeKI GCWGC 4 cut(s) 37, 116, 271, 327
AspS9I GGNCC 1 cut(s) 186
AsuC2I CCSGG 2 cut(s) 163, 354
AsuHPI GGTGA 1 cut(s) 287
BaeGI GKGCMC 1 cut(s) 376
BamHI GGATCC 1 cut(s) 504
BanI GGYRCC 2 cut(s) 281, 371
BbvI GCAGC 4 cut(s) 49, 103, 258, 339
BciT130I CCWGG 1 cut(s) 241
BcnI CCSGG 2 cut(s) 163, 354
BfaI CTAG 1 cut(s) 390
BfmI CTRYAG 1 cut(s) 117
BisI GCNGC 5 cut(s) 38, 117, 272, 285, 328
BlsI GCNGC 5 cut(s) 39, 118, 273, 286, 329
Bme1390I CCNGG 3 cut(s) 163, 241, 354
BmgT120I GGNCC 1 cut(s) 186
BmiI GGNNCC 4 cut(s) 188, 283, 373, 506
BmrFI CCNGG 3 cut(s) 163, 241, 354
BpmI CTGGAG 1 cut(s) 387
BpuMI CCSGG 2 cut(s) 163, 354
BsaJI CCNNGG 1 cut(s) 500
Bsc4I CCNNNNNNNGG 2 cut(s) 98, 476
Bse118I RCCGGY 1 cut(s) 297
BseBI CCWGG 1 cut(s) 241
BseDI CCNNGG 1 cut(s) 500
BseLI CCNNNNNNNGG 2 cut(s) 98, 476
BseMII CTCAG 2 cut(s) 59, 444
BseSI GKGCMC 1 cut(s) 376
BseXI GCAGC 4 cut(s) 49, 103, 258, 339
BseYI CCCAGC 1 cut(s) 189
BshFI GGCC 1 cut(s) 187
BshNI GGYRCC 2 cut(s) 281, 371
BsiSI CCGG 4 cut(s) 92, 162, 298, 354
BslI CCNNNNNNNGG 2 cut(s) 98, 476
BsmI GAATGC 1 cut(s) 248
BsnI GGCC 1 cut(s) 187
Bsp1286I GDGCHC 1 cut(s) 376
Bsp143I GATC 1 cut(s) 504
BspACI CCGC 4 cut(s) 35, 285, 459, 509
BspANI GGCC 1 cut(s) 187
BspCNI CTCAG 2 cut(s) 58, 443
BspLI GGNNCC 4 cut(s) 188, 283, 373, 506
BspMAI CTGCAG 1 cut(s) 121
BspPI GGATC 2 cut(s) 499, 512
BspT107I GGYRCC 2 cut(s) 281, 371
BsrFI RCCGGY 1 cut(s) 297
BssAI RCCGGY 1 cut(s) 297
BssECI CCNNGG 1 cut(s) 500
BssMI GATC 1 cut(s) 504
Bst2UI CCWGG 1 cut(s) 241
Bst4CI ACNGT 4 cut(s) 214, 222, 338, 443
BstAPI GCANNNNNTGC 1 cut(s) 174
BstC8I GCNNGC 1 cut(s) 276
BstDEI CTNAG 2 cut(s) 45, 430
BstENI CCTNNNNNAGG 1 cut(s) 474
BstKTI GATC 1 cut(s) 507
BstMBI GATC 1 cut(s) 504
BstMWI GCNNNNNNNGC 4 cut(s) 122, 174, 284, 371
BstNI CCWGG 1 cut(s) 241
BstNSI RCATGY 1 cut(s) 75
BstSCI CCNGG 3 cut(s) 161, 239, 352
BstSFI CTRYAG 1 cut(s) 117
BstSLI GKGCMC 1 cut(s) 376
BstV1I GCAGC 4 cut(s) 49, 103, 258, 339
BstX2I RGATCY 1 cut(s) 504
BstYI RGATCY 1 cut(s) 504
BsuRI GGCC 1 cut(s) 187
BtsI GCAGTG 1 cut(s) 126
BtsIMutI CAGTG 2 cut(s) 126, 448
Cac8I GCNNGC 1 cut(s) 276
Cfr10I RCCGGY 1 cut(s) 297
Cfr13I GGNCC 1 cut(s) 186
Csp6I GTAC 1 cut(s) 398
CviAII CATG 2 cut(s) 72, 302
CviJI RGCY 7 cut(s) 95, 125, 137, 187, 193, 330, 488
CviKI_1 RGCY 7 cut(s) 95, 125, 137, 187, 193, 330, 488
CviQI GTAC 1 cut(s) 398
DdeI CTNAG 2 cut(s) 45, 430
DpnI GATC 1 cut(s) 506
DpnII GATC 1 cut(s) 504
EcoNI CCTNNNNNAGG 1 cut(s) 474
EcoO109I RGGNCCY 1 cut(s) 186
EcoRII CCWGG 1 cut(s) 239
FaeI CATG 2 cut(s) 75, 305
FaiI YATR 9 cut(s) 73, 131, 149, 257, 303, 319, 417, 449, 491
FatI CATG 2 cut(s) 71, 301
FauI CCCGC 2 cut(s) 42, 516
FblI GTMKAC 1 cut(s) 411
Fnu4HI GCNGC 5 cut(s) 38, 117, 272, 285, 328
Fsp4HI GCNGC 5 cut(s) 38, 117, 272, 285, 328
FspBI CTAG 1 cut(s) 390
GluI GCNGC 5 cut(s) 38, 117, 272, 285, 328
GsaI CCCAGC 1 cut(s) 193
GsuI CTGGAG 1 cut(s) 387
HaeIII GGCC 1 cut(s) 187
HapII CCGG 4 cut(s) 92, 162, 298, 354
Hin1II CATG 2 cut(s) 75, 305
HinfI GANTC 1 cut(s) 59
HpaII CCGG 4 cut(s) 92, 162, 298, 354
HphI GGTGA 1 cut(s) 287
Hpy166II GTNNAC 2 cut(s) 412, 421
Hpy188I TCNGA 2 cut(s) 404, 504
Hpy8I GTNNAC 2 cut(s) 412, 421
Hpy99I CGWCG 1 cut(s) 214
HpyAV CCTTC 2 cut(s) 174, 204
HpyCH4III ACNGT 4 cut(s) 214, 222, 338, 443
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 3 cut(s) 71, 119, 168
HpyF10VI GCNNNNNNNGC 4 cut(s) 122, 174, 284, 371
HpyF3I CTNAG 2 cut(s) 45, 430
HpySE526I ACGT 1 cut(s) 342
Hsp92II CATG 2 cut(s) 75, 305
Kzo9I GATC 1 cut(s) 504
Lsp1109I GCAGC 4 cut(s) 49, 103, 258, 339
MaeI CTAG 1 cut(s) 390
MaeII ACGT 1 cut(s) 342
MaeIII GTNAC 4 cut(s) 16, 109, 222, 338
MalI GATC 1 cut(s) 506
MboI GATC 1 cut(s) 504
MfeI CAATTG 1 cut(s) 169
MflI RGATCY 1 cut(s) 504
MhlI GDGCHC 1 cut(s) 376
MluCI AATT 3 cut(s) 144, 169, 405
MlyI GAGTC 1 cut(s) 53
MnlI CCTC 4 cut(s) 177, 362, 470, 510
MseI TTAA 1 cut(s) 425
MslI CAYNNNNRTG 1 cut(s) 300
MspI CCGG 4 cut(s) 92, 162, 298, 354
MspR9I CCNGG 3 cut(s) 163, 241, 354
MunI CAATTG 1 cut(s) 169
Mva1269I GAATGC 1 cut(s) 248
MvaI CCWGG 1 cut(s) 241
MwoI GCNNNNNNNGC 4 cut(s) 122, 174, 284, 371
NciI CCSGG 2 cut(s) 163, 354
NdeII GATC 1 cut(s) 504
NlaIII CATG 2 cut(s) 75, 305
NlaIV GGNNCC 4 cut(s) 188, 283, 373, 506
NspI RCATGY 1 cut(s) 75
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PctI GAATGC 1 cut(s) 248
PkrI GCNGC 5 cut(s) 39, 118, 273, 286, 329
PleI GAGTC 1 cut(s) 53
PpsI GAGTC 1 cut(s) 53
PsiI TTATAA 1 cut(s) 149
Psp6I CCWGG 1 cut(s) 239
PspFI CCCAGC 1 cut(s) 189
PspGI CCWGG 1 cut(s) 239
PspN4I GGNNCC 4 cut(s) 188, 283, 373, 506
PspPI GGNCC 1 cut(s) 186
PstI CTGCAG 1 cut(s) 121
PsuI RGATCY 1 cut(s) 504
RsaI GTAC 1 cut(s) 399
RsaNI GTAC 1 cut(s) 398
RseI CAYNNNNRTG 1 cut(s) 300
SaqAI TTAA 1 cut(s) 425
SatI GCNGC 5 cut(s) 38, 117, 272, 285, 328
Sau3AI GATC 1 cut(s) 504
Sau96I GGNCC 1 cut(s) 186
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 3 cut(s) 163, 241, 354
SduI GDGCHC 1 cut(s) 376
SetI ASST 7 cut(s) 51, 139, 332, 345, 373, 490, 502
SfcI CTRYAG 1 cut(s) 117
SmiMI CAYNNNNRTG 1 cut(s) 300
Sse9I AATT 3 cut(s) 144, 169, 405
SsiI CCGC 4 cut(s) 35, 285, 459, 509
SspMI CTAG 1 cut(s) 390
StyD4I CCNGG 3 cut(s) 161, 239, 352
TaaI ACNGT 4 cut(s) 214, 222, 338, 443
TaiI ACGT 1 cut(s) 345
TaqI TCGA 3 cut(s) 62, 209, 475
TasI AATT 3 cut(s) 144, 169, 405
TauI GCSGC 1 cut(s) 287
Tru1I TTAA 1 cut(s) 425
Tru9I TTAA 1 cut(s) 425
TscAI CASTG 2 cut(s) 126, 448
TseI GCWGC 4 cut(s) 37, 116, 271, 327
TspDTI ATGAA 1 cut(s) 41
TspRI CASTG 2 cut(s) 126, 448
XagI CCTNNNNNAGG 1 cut(s) 474
XceI RCATGY 1 cut(s) 75
XmiI GTMKAC 1 cut(s) 411
XspI CTAG 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.