pycom02g00280

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0028573
Physical Location & Seq
Forward (+)
164072 .. 167673
3602 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1515 bp
ATGCCTACGCAATCGGAGAACGGGATTCCGGTGATCGATTTCTCCAAGGAGGAGGAGTTGAAGCCGGGGTCGAGTTCTTGGCTAGGGATACGCGAAGATGTTATCCGTGCACTCGAAGGAACGGGCTGCTTCATTGCATTAGTCGGCGACAAGGTCCCCCAAGAGCTTCGCAGCACCATGTTGGAGGGTTTCAAAGAGTTGATGGATTTCCCCCGTGAAGCCAAGATGAAGGTCAAGAGCAAGACCAAGAAGACCTTGGGTGCATATCACAACGCCACTCAGATCGATGAAAGCCTGGCAATGGACGACGCCGCAGATGCTGCTGAATGCCAGAAGTTCAGCAATCTCTTCTGGCCCAACGGAAATGAAAATTTCACTCGAAGTTCGAACGAGTATGCAACGCAAATGATGAACTTAGACTGGATAGTGACAAGGATGATATTCGAAAGCTATGGTGTAGACCAGTACCATGATACACATATTAACACCAACACTCACCACATGTCGTTCCTCAAGTACGAGCGATCGGAGGAAATTGAAACCGACGTGGGTGTTTTTATTCACACCGACAAGAACTTCACCACGGTGCTGCATCAGGTGAACGACAGCCATGGCTTTGAGGTGCAGACCAAGAACGGGGACTGGGTGCTCTGTCAACCCCCGCCATCGTCCTTCACTTTCATCGCAGCCGATATTTTGCAGGTTTGGAGCAATGATAAAATACAAGCTTGCAAACACAGAGTGACAATAAGCAAAGATACGCAAAGGTTCGTTTTGGCTCTGTTTCTGTTCAAGAGTGGCAAGTGCCAGACGCCGGAAGAGTTGATTGACGAGGAGCATCCCGTCCGCTACAGACCGATTGATCAATCTGAATATTTTACCGACTTTCTCAACAACGCAAGAAATGGAGGAATTAGTTACACTATTAATAACATAGACAACTCAAAATGGAAACTGGATCCTCTCCTAGATCAGGATGGGGATTCTCCTGAAACTGAAGCGAAGTGGAAGGTTGAAAGCAGGAACAGACGAGTGTCCACCCGCTGCGACGCGGCCGTTGCCGGGGAGGCCACTGATGCTACTGGTGAGAAATTCGAGTACCAGGTCGAGATTCGTATCAAATCTGATCCAGATAATGGGACCATCACTATATCGGGTACTGGTATTGGAATGACAAAAGAGGAACTCATTGGCTTCATTGGAACCATTGCCCAGAGTGGTACTTCAAAGTTCTTGAAGGCTCTGAAGGGAAACAAGGATCTTGGTGCAGACAATGGCTTGATTGGTCAATTTGGTGTTGGGTTCTACCCTGCTTTTCTTGTTGCTGGGAAGGTGGTTGTCTCTACAAAAAGCACAAGGAAAGTGTGGTGTGCGTGGATGCAAAGGGATTGGAGCTCAAAGATGATCGATCTTCACCTCACCACCAAATCCCAGGTTCAGTTGGGTCACATGTTGGCTAATGCATACCTCCAGCATTTTGGACCCTCAGAAGCCAATTCTCATGCTACCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

505

Amino Acids

56.64

Weight (kDa)

5.47

Isoelectric Point (pI)

32.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 9 - 103 7.2e-08 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 168 - 264 6.7e-16 2OG-Fe(II) oxygenase superfamily
HATPase_c_3 PF13589 368 - 472 6.2e-06 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028573)

Species Orthologous Gene IDs
malus_domestica MD02G1004300.v1.1
pyrus_communis pycom02g00280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 691
AccB7I CCANNNNNTGG 1 cut(s) 1136
AccI GTMKAC 1 cut(s) 459
AccII CGCG 2 cut(s) 93, 1052
AciI CCGC 5 cut(s) 312, 662, 847, 1042, 1052
AclWI GGATC 4 cut(s) 953, 966, 1121, 1266
AcoI YGGCCR 1 cut(s) 1053
AcsI RAATTY 2 cut(s) 370, 1091
AcuI CTGAAG 2 cut(s) 1017, 1265
AcyI GRCGYC 2 cut(s) 309, 812
AdeI CACNNNGTG 1 cut(s) 742
AfaI GTAC 5 cut(s) 467, 518, 1100, 1159, 1222
AfiI CCNNNNNNNGG 6 cut(s) 301, 636, 814, 973, 1062, 1136
AflIII ACRYGT 2 cut(s) 501, 1449
AgsI TTSAA 7 cut(s) 61, 193, 539, 793, 1016, 1227, 1237
AjiI CACGTC 1 cut(s) 547
AjnI CCWGG 3 cut(s) 294, 1101, 1431
AleI CACNNNNGTG 1 cut(s) 584
AluBI AGCT 4 cut(s) 166, 450, 728, 1395
AluI AGCT 4 cut(s) 166, 450, 728, 1395
Alw21I GWGCWC 3 cut(s) 112, 651, 1397
Alw26I GTCTC 1 cut(s) 1345
Alw44I GTGCAC 1 cut(s) 108
AlwI GGATC 4 cut(s) 953, 966, 1121, 1266
AlwNI CAGNNNCTG 1 cut(s) 320
AoxI GGCC 3 cut(s) 353, 1053, 1068
ApaLI GTGCAC 1 cut(s) 108
ApeKI GCWGC 6 cut(s) 126, 171, 320, 589, 686, 1044
ApoI RAATTY 2 cut(s) 370, 1091
AseI ATTAAT 1 cut(s) 927
AspS9I GGNCC 4 cut(s) 154, 354, 1140, 1481
AsuC2I CCSGG 2 cut(s) 66, 1063
AsuHPI GGTGA 7 cut(s) 43, 488, 571, 610, 1097, 1406, 1411
AsuII TTCGAA 2 cut(s) 386, 444
AvaII GGWCC 3 cut(s) 154, 1140, 1481
BaeGI GKGCMC 1 cut(s) 112
BamHI GGATCC 1 cut(s) 958
BanII GRGCYC 1 cut(s) 1397
BbsI GAAGAC 1 cut(s) 257
Bbv12I GWGCWC 3 cut(s) 112, 651, 1397
BbvI GCAGC 6 cut(s) 113, 183, 307, 576, 698, 1031
BccI CCATC 4 cut(s) 196, 673, 971, 1151
BceAI ACGGC 1 cut(s) 1040
BciT130I CCWGG 3 cut(s) 296, 1103, 1433
BciVI GTATCC 1 cut(s) 81
BclI TGATCA 1 cut(s) 862
BcnI CCSGG 2 cut(s) 66, 1063
BcoDI GTCTC 1 cut(s) 1345
BfaI CTAG 2 cut(s) 83, 968
BfmI CTRYAG 1 cut(s) 850
BfuAI ACCTGC 1 cut(s) 691
BfuI GTATCC 1 cut(s) 81
BglI GCCNNNNNGGC 1 cut(s) 1067
BisI GCNGC 8 cut(s) 127, 172, 312, 321, 590, 687, 1045, 1053
BlsI GCNGC 8 cut(s) 128, 173, 313, 322, 591, 688, 1046, 1054
Bme1390I CCNGG 5 cut(s) 66, 296, 1063, 1103, 1433
Bme18I GGWCC 3 cut(s) 154, 1140, 1481
BmgBI CACGTC 1 cut(s) 547
BmgT120I GGNCC 4 cut(s) 154, 354, 1140, 1481
BmiI GGNNCC 5 cut(s) 156, 960, 1141, 1204, 1483
BmrFI CCNGG 5 cut(s) 66, 296, 1063, 1103, 1433
BmrI ACTGGG 1 cut(s) 652
BmsI GCATC 5 cut(s) 307, 601, 847, 1066, 1368
BmuI ACTGGG 1 cut(s) 652
BoxI GACNNNNGTC 1 cut(s) 1033
BpiI GAAGAC 1 cut(s) 257
BpmI CTGGAG 1 cut(s) 1454
Bpu14I TTCGAA 2 cut(s) 386, 444
BpuEI CTTGAG 1 cut(s) 497
BpuMI CCSGG 2 cut(s) 66, 1063
Bsa29I ATCGAT 3 cut(s) 36, 285, 1407
BsaBI GATNNNNATC 1 cut(s) 1115
BsaHI GRCGYC 2 cut(s) 309, 812
BsaJI CCNNGG 7 cut(s) 45, 65, 255, 582, 610, 1062, 1431
BsaWI WCCGGW 1 cut(s) 28
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 6 cut(s) 301, 636, 814, 973, 1062, 1136
Bse1I ACTGG 6 cut(s) 425, 463, 647, 960, 1087, 1165
Bse3DI GCAATG 4 cut(s) 132, 306, 718, 1206
Bse8I GATNNNNATC 1 cut(s) 1115
BseBI CCWGG 3 cut(s) 296, 1103, 1433
BseCI ATCGAT 3 cut(s) 36, 285, 1407
BseDI CCNNGG 7 cut(s) 45, 65, 255, 582, 610, 1062, 1431
BseGI GGATG 4 cut(s) 441, 838, 982, 1383
BseJI GATNNNNATC 1 cut(s) 1115
BseLI CCNNNNNNNGG 6 cut(s) 301, 636, 814, 973, 1062, 1136
BseMI GCAATG 4 cut(s) 132, 306, 718, 1206
BseMII CTCAG 2 cut(s) 293, 1500
BseNI ACTGG 6 cut(s) 425, 463, 647, 960, 1087, 1165
BseRI GAGGAG 3 cut(s) 65, 68, 848
BseSI GKGCMC 1 cut(s) 112
BseX3I CGGCCG 1 cut(s) 1053
BseXI GCAGC 6 cut(s) 113, 183, 307, 576, 698, 1031
BseYI CCCAGC 1 cut(s) 1325
BsgI GTGCAG 2 cut(s) 644, 1287
Bsh1236I CGCG 2 cut(s) 93, 1052
Bsh1285I CGRYCG 2 cut(s) 527, 1056
BshFI GGCC 3 cut(s) 355, 1055, 1070
BshVI ATCGAT 3 cut(s) 36, 285, 1407
BsiEI CGRYCG 2 cut(s) 527, 1056
BsiHKAI GWGCWC 3 cut(s) 112, 651, 1397
BsiSI CCGG 4 cut(s) 29, 65, 815, 1062
BslFI GGGAC 3 cut(s) 140, 653, 1153
BslI CCNNNNNNNGG 6 cut(s) 301, 636, 814, 973, 1062, 1136
BsmAI GTCTC 1 cut(s) 1345
BsmFI GGGAC 3 cut(s) 140, 653, 1153
BsmI GAATGC 1 cut(s) 332
BsnI GGCC 3 cut(s) 355, 1055, 1070
Bsp119I TTCGAA 2 cut(s) 386, 444
Bsp1286I GDGCHC 3 cut(s) 112, 651, 1397
Bsp19I CCATGG 1 cut(s) 610
BspACI CCGC 5 cut(s) 312, 662, 847, 1042, 1052
BspANI GGCC 3 cut(s) 355, 1055, 1070
BspCNI CTCAG 2 cut(s) 292, 1499
BspDI ATCGAT 3 cut(s) 36, 285, 1407
BspFNI CGCG 2 cut(s) 93, 1052
BspLI GGNNCC 5 cut(s) 156, 960, 1141, 1204, 1483
BspMI ACCTGC 1 cut(s) 691
BspPI GGATC 4 cut(s) 953, 966, 1121, 1266
BspT104I TTCGAA 2 cut(s) 386, 444
BsrDI GCAATG 4 cut(s) 132, 306, 718, 1206
BsrI ACTGG 6 cut(s) 425, 463, 647, 960, 1087, 1165
BssECI CCNNGG 7 cut(s) 45, 65, 255, 582, 610, 1062, 1431
BssNI GRCGYC 2 cut(s) 309, 812
BssT1I CCWWGG 3 cut(s) 45, 255, 610
Bst2UI CCWGG 3 cut(s) 296, 1103, 1433
Bst4CI ACNGT 1 cut(s) 586
Bst6I CTCTTC 2 cut(s) 353, 813
BstACI GRCGYC 2 cut(s) 309, 812
BstAPI GCANNNNNTGC 1 cut(s) 320
BstBI TTCGAA 2 cut(s) 386, 444
BstC8I GCNNGC 1 cut(s) 730
BstDEI CTNAG 3 cut(s) 279, 415, 1486
BstDSI CCRYGG 2 cut(s) 582, 610
BstENI CCTNNNNNAGG 1 cut(s) 971
BstF5I GGATG 4 cut(s) 441, 838, 982, 1383
BstFNI CGCG 2 cut(s) 93, 1052
BstMAI GTCTC 1 cut(s) 1345
BstMCI CGRYCG 2 cut(s) 527, 1056
BstMWI GCNNNNNNNGC 5 cut(s) 317, 320, 1058, 1067, 1076
BstNI CCWGG 3 cut(s) 296, 1103, 1433
BstNSI RCATGY 2 cut(s) 505, 1453
BstPAI GACNNNNGTC 1 cut(s) 1033
BstSCI CCNGG 5 cut(s) 64, 294, 1061, 1101, 1431
BstSFI CTRYAG 1 cut(s) 850
BstSLI GKGCMC 1 cut(s) 112
BstUI CGCG 2 cut(s) 93, 1052
BstV1I GCAGC 6 cut(s) 113, 183, 307, 576, 698, 1031
BstV2I GAAGAC 1 cut(s) 257
BstX2I RGATCY 2 cut(s) 958, 1258
BstXI CCANNNNNNTGG 1 cut(s) 1478
BstYI RGATCY 2 cut(s) 958, 1258
BstZI CGGCCG 1 cut(s) 1053
Bsu15I ATCGAT 3 cut(s) 36, 285, 1407
BsuI GTATCC 1 cut(s) 81
BsuRI GGCC 3 cut(s) 355, 1055, 1070
BsuTUI ATCGAT 3 cut(s) 36, 285, 1407
BtgI CCRYGG 2 cut(s) 582, 610
BtgZI GCGATG 1 cut(s) 667
BtrI CACGTC 1 cut(s) 547
BtsCI GGATG 4 cut(s) 441, 838, 982, 1383
BtsIMutI CAGTG 1 cut(s) 1071
BveI ACCTGC 1 cut(s) 691
Cac8I GCNNGC 1 cut(s) 730
CaiI CAGNNNCTG 1 cut(s) 320
Cfr13I GGNCC 4 cut(s) 154, 354, 1140, 1481
ClaI ATCGAT 3 cut(s) 36, 285, 1407
CseI GACGC 3 cut(s) 317, 820, 1058
CsiI ACCWGGT 1 cut(s) 1101
Csp6I GTAC 5 cut(s) 466, 517, 1099, 1158, 1221
CviAII CATG 6 cut(s) 178, 470, 502, 611, 1450, 1502
CviQI GTAC 5 cut(s) 466, 517, 1099, 1158, 1221
DdeI CTNAG 3 cut(s) 279, 415, 1486
DraIII CACNNNGTG 1 cut(s) 742
EaeI YGGCCR 1 cut(s) 1053
EagI CGGCCG 1 cut(s) 1053
Eam1104I CTCTTC 2 cut(s) 353, 813
EarI CTCTTC 2 cut(s) 353, 813
Ecl136II GAGCTC 1 cut(s) 1395
EclXI CGGCCG 1 cut(s) 1053
Eco130I CCWWGG 3 cut(s) 45, 255, 610
Eco24I GRGCYC 1 cut(s) 1397
Eco47I GGWCC 3 cut(s) 154, 1140, 1481
Eco52I CGGCCG 1 cut(s) 1053
Eco53kI GAGCTC 1 cut(s) 1395
Eco57I CTGAAG 2 cut(s) 1017, 1265
EcoICRI GAGCTC 1 cut(s) 1395
EcoNI CCTNNNNNAGG 1 cut(s) 971
EcoO109I RGGNCCY 1 cut(s) 154
EcoRII CCWGG 3 cut(s) 294, 1101, 1431
EcoT14I CCWWGG 3 cut(s) 45, 255, 610
EcoT22I ATGCAT 1 cut(s) 1465
EcoT38I GRGCYC 1 cut(s) 1397
ErhI CCWWGG 3 cut(s) 45, 255, 610
FaeI CATG 6 cut(s) 181, 473, 505, 614, 1453, 1505
FalI AAGNNNNNCTT 2 cut(s) 239, 271
FaqI GGGAC 3 cut(s) 140, 653, 1153
FatI CATG 6 cut(s) 177, 469, 501, 610, 1449, 1501
FauI CCCGC 2 cut(s) 669, 1049
FbaI TGATCA 1 cut(s) 862
FblI GTMKAC 1 cut(s) 459
Fnu4HI GCNGC 8 cut(s) 127, 172, 312, 321, 590, 687, 1045, 1053
FokI GGATG 4 cut(s) 448, 825, 989, 1390
FriOI GRGCYC 1 cut(s) 1397
Fsp4HI GCNGC 8 cut(s) 127, 172, 312, 321, 590, 687, 1045, 1053
FspBI CTAG 2 cut(s) 83, 968
GluI GCNGC 8 cut(s) 127, 172, 312, 321, 590, 687, 1045, 1053
GsaI CCCAGC 1 cut(s) 1329
GsuI CTGGAG 1 cut(s) 1454
HaeIII GGCC 3 cut(s) 355, 1055, 1070
HapII CCGG 4 cut(s) 29, 65, 815, 1062
HgaI GACGC 3 cut(s) 317, 820, 1058
Hin1I GRCGYC 2 cut(s) 309, 812
Hin1II CATG 6 cut(s) 181, 473, 505, 614, 1453, 1505
HincII GTYRAC 1 cut(s) 656
HindII GTYRAC 1 cut(s) 656
HindIII AAGCTT 1 cut(s) 726
HinfI GANTC 3 cut(s) 25, 983, 1111
HpaII CCGG 4 cut(s) 29, 65, 815, 1062
HphI GGTGA 7 cut(s) 43, 488, 571, 610, 1097, 1406, 1411
Hpy166II GTNNAC 5 cut(s) 110, 460, 601, 656, 1038
Hpy188I TCNGA 7 cut(s) 16, 282, 529, 871, 1126, 1245, 1489
Hpy188III TCNNGA 7 cut(s) 235, 793, 974, 989, 1108, 1130, 1234
Hpy8I GTNNAC 5 cut(s) 110, 460, 601, 656, 1038
Hpy99I CGWCG 3 cut(s) 311, 548, 1052
HpyAV CCTTC 7 cut(s) 110, 223, 682, 1003, 1231, 1240, 1324
HpyCH4III ACNGT 1 cut(s) 586
HpyCH4IV ACGT 1 cut(s) 546
HpyF10VI GCNNNNNNNGC 5 cut(s) 317, 320, 1058, 1067, 1076
HpyF3I CTNAG 3 cut(s) 279, 415, 1486
HpySE526I ACGT 1 cut(s) 546
Hsp92I GRCGYC 2 cut(s) 309, 812
Hsp92II CATG 6 cut(s) 181, 473, 505, 614, 1453, 1505
Ksp22I TGATCA 1 cut(s) 862
LmnI GCTCC 3 cut(s) 708, 835, 1392
Lsp1109I GCAGC 6 cut(s) 113, 183, 307, 576, 698, 1031
LweI GCATC 5 cut(s) 307, 601, 847, 1066, 1368
MabI ACCWGGT 1 cut(s) 1101
MaeI CTAG 2 cut(s) 83, 968
MaeII ACGT 1 cut(s) 546
MaeIII GTNAC 4 cut(s) 427, 742, 917, 1445
MboII GAAGA 5 cut(s) 107, 262, 340, 830, 1403
MflI RGATCY 2 cut(s) 958, 1258
MhlI GDGCHC 3 cut(s) 112, 651, 1397
MluCI AATT 6 cut(s) 370, 534, 912, 1091, 1289, 1495
MmeI TCCRAC 1 cut(s) 162
Mph1103I ATGCAT 1 cut(s) 1465
MseI TTAA 2 cut(s) 483, 927
MslI CAYNNNNRTG 1 cut(s) 584
MspA1I CMGCKG 1 cut(s) 1044
MspI CCGG 4 cut(s) 29, 65, 815, 1062
MspR9I CCNGG 5 cut(s) 66, 296, 1063, 1103, 1433
Mva1269I GAATGC 1 cut(s) 332
MvaI CCWGG 3 cut(s) 296, 1103, 1433
MvnI CGCG 2 cut(s) 93, 1052
MwoI GCNNNNNNNGC 5 cut(s) 317, 320, 1058, 1067, 1076
NciI CCSGG 2 cut(s) 66, 1063
NcoI CCATGG 1 cut(s) 610
NlaIII CATG 6 cut(s) 181, 473, 505, 614, 1453, 1505
NlaIV GGNNCC 5 cut(s) 156, 960, 1141, 1204, 1483
NmuCI GTSAC 3 cut(s) 427, 742, 1445
NsiI ATGCAT 1 cut(s) 1465
NspI RCATGY 2 cut(s) 505, 1453
NspV TTCGAA 2 cut(s) 386, 444
OliI CACNNNNGTG 1 cut(s) 584
PciI ACATGT 2 cut(s) 501, 1449
PctI GAATGC 1 cut(s) 332
PfeI GAWTC 3 cut(s) 25, 983, 1111
PflFI GACNNNGTC 1 cut(s) 152
PflMI CCANNNNNTGG 1 cut(s) 1136
PkrI GCNGC 8 cut(s) 128, 173, 313, 322, 591, 688, 1046, 1054
Ple19I CGATCG 1 cut(s) 527
PpuMI RGGWCCY 1 cut(s) 154
PscI ACATGT 2 cut(s) 501, 1449
PshAI GACNNNNGTC 1 cut(s) 1033
PshBI ATTAAT 1 cut(s) 927
Psp124BI GAGCTC 1 cut(s) 1397
Psp5II RGGWCCY 1 cut(s) 154
Psp6I CCWGG 3 cut(s) 294, 1101, 1431
PspFI CCCAGC 1 cut(s) 1325
PspGI CCWGG 3 cut(s) 294, 1101, 1431
PspN4I GGNNCC 5 cut(s) 156, 960, 1141, 1204, 1483
PspPI GGNCC 4 cut(s) 154, 354, 1140, 1481
PspPPI RGGWCCY 1 cut(s) 154
PstNI CAGNNNCTG 1 cut(s) 320
PsuI RGATCY 2 cut(s) 958, 1258
PsyI GACNNNGTC 1 cut(s) 152
PvuI CGATCG 1 cut(s) 527
RsaI GTAC 5 cut(s) 467, 518, 1100, 1159, 1222
RsaNI GTAC 5 cut(s) 466, 517, 1099, 1158, 1221
RseI CAYNNNNRTG 1 cut(s) 584
SacI GAGCTC 1 cut(s) 1397
SaqAI TTAA 2 cut(s) 483, 927
SatI GCNGC 8 cut(s) 127, 172, 312, 321, 590, 687, 1045, 1053
Sau96I GGNCC 4 cut(s) 154, 354, 1140, 1481
ScrFI CCNGG 5 cut(s) 66, 296, 1063, 1103, 1433
SduI GDGCHC 3 cut(s) 112, 651, 1397
SexAI ACCWGGT 1 cut(s) 1101
SfaNI GCATC 5 cut(s) 307, 601, 847, 1066, 1368
SfcI CTRYAG 1 cut(s) 850
SfuI TTCGAA 2 cut(s) 386, 444
SinI GGWCC 3 cut(s) 154, 1140, 1481
SmiMI CAYNNNNRTG 1 cut(s) 584
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
Sse9I AATT 6 cut(s) 370, 534, 912, 1091, 1289, 1495
SsiI CCGC 5 cut(s) 312, 662, 847, 1042, 1052
SspI AATATT 1 cut(s) 875
SspMI CTAG 2 cut(s) 83, 968
SstI GAGCTC 1 cut(s) 1397
StyD4I CCNGG 5 cut(s) 64, 294, 1061, 1101, 1431
StyI CCWWGG 3 cut(s) 45, 255, 610
TaaI ACNGT 1 cut(s) 586
TaiI ACGT 1 cut(s) 549
TaqII GACCGA 1 cut(s) 871
TasI AATT 6 cut(s) 370, 534, 912, 1091, 1289, 1495
TauI GCSGC 2 cut(s) 314, 1055
TfiI GAWTC 3 cut(s) 25, 983, 1111
Tru1I TTAA 2 cut(s) 483, 927
Tru9I TTAA 2 cut(s) 483, 927
TscAI CASTG 1 cut(s) 1078
TseFI GTSAC 3 cut(s) 427, 742, 1445
TseI GCWGC 6 cut(s) 126, 171, 320, 589, 686, 1044
Tsp45I GTSAC 3 cut(s) 427, 742, 1445
TspDTI ATGAA 7 cut(s) 121, 242, 303, 381, 425, 670, 1186
TspGWI ACGGA 2 cut(s) 95, 375
TspRI CASTG 1 cut(s) 1078
Tth111I GACNNNGTC 1 cut(s) 152
Van91I CCANNNNNTGG 1 cut(s) 1136
VneI GTGCAC 1 cut(s) 108
VpaK11BI GGWCC 3 cut(s) 154, 1140, 1481
VspI ATTAAT 1 cut(s) 927
XagI CCTNNNNNAGG 1 cut(s) 971
XapI RAATTY 2 cut(s) 370, 1091
XceI RCATGY 2 cut(s) 505, 1453
XcmI CCANNNNNNNNNTGG 1 cut(s) 253
XmiI GTMKAC 1 cut(s) 459
XspI CTAG 2 cut(s) 83, 968
Zsp2I ATGCAT 1 cut(s) 1465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.