pycom02g01650

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0029208
Physical Location & Seq
Forward (+)
977624 .. 978644
1021 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 921 bp
ATGAAGCTTTATTCGGAGGCAATGGAGGAGACAATCACAAGTGAAGAAGCACAGGACCTGTTTAACATAGCAGGAGAGAAGTTCCAAGAGATGGGAGCTTTAGCTCTGTTCAACTGGGGAAATGTTCACATGGCCAGGGCGAGGAAGAAGTTGTATTTCACCGAAGATTCTTCGAAAGAATCTATCATTTCAAATATCAAAAGTGCATATGATTGGGCACAGAAAGAATACATAGAATCAGGAAGGAGATACGAAGAAGCGCTGCAGATCAAACCAGACTTTTATGAAGGCTATTTGGCCCTCGGGCAGCAGCAGTTTGAGCAGGCAAAACTTTCTTGGTATTATGCAATTAGCAGCAATGTTGATCTGGAGACGTGGCCTTCCACAGAGGTTCTGCGACTTTATAACAATGCCGAAGACAATATGGAAAAAGGTATGCAGCTGTTCGAGGAATTGGAAGAGCAACGCCTGACTGAACTCTCCTGTCCAAGTAGTGTCAAAACACAGGTGCAGAAAATGGGTTTGAATGGAATTTTCAAAGACGTATCATCAAATGAAGCTGCAGACCAGGCTACAAGCATGAGATGTCAGATCAATCTCCTATGGGGCACTATGCTCTACGAGCGATCTATTGTGGAATTCAAGTTAGGGCTACCTGTATGGAATGAATGTCTAGACGTCGCAGTTGAAAAATTTGGCCTAGCCGGGGCTTCACCCACAGATATAGCAGTCATGCTCAAAAACCACTCTTCAAATGATAATGCATTAGAAGGTCTGGGGTTCAAAATTGACGAGATAGTTCAAGCTTGGAACGAGATGCAAGAAGCGAAAAAGTGGCAGACTGGAATTCCATCATTCCGGTTAGAACCATTACTGCGAAGACGAGTTTCCAGAATTTATTCTGCCTTGGAGTATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

307

Amino Acids

35.13

Weight (kDa)

4.78

Isoelectric Point (pI)

46.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029208)

Species Orthologous Gene IDs
pyrus_communis pycom02g01650
rosa_wichuraiana Rw2G002020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 405
AatII GACGTC 1 cut(s) 681
AccB7I CCANNNNNTGG 1 cut(s) 91
AcoI YGGCCR 1 cut(s) 132
AcsI RAATTY 5 cut(s) 531, 638, 692, 846, 894
AcyI GRCGYC 1 cut(s) 678
AfeI AGCGCT 1 cut(s) 261
AfiI CCNNNNNNNGG 3 cut(s) 91, 141, 706
AgsI TTSAA 9 cut(s) 112, 192, 526, 538, 643, 689, 753, 784, 803
AjiI CACGTC 1 cut(s) 375
AjnI CCWGG 2 cut(s) 134, 567
AluBI AGCT 6 cut(s) 7, 98, 104, 442, 560, 806
AluI AGCT 6 cut(s) 7, 98, 104, 442, 560, 806
Alw26I GTCTC 2 cut(s) 23, 365
AlwNI CAGNNNCTG 1 cut(s) 58
Ama87I CYCGRG 1 cut(s) 302
Aor51HI AGCGCT 1 cut(s) 261
AoxI GGCC 4 cut(s) 132, 297, 377, 697
ApeKI GCWGC 6 cut(s) 262, 307, 310, 354, 439, 560
ApoI RAATTY 5 cut(s) 531, 638, 692, 846, 894
Asp700I GAANNNNTTC 1 cut(s) 898
AspLEI GCGC 1 cut(s) 262
AspS9I GGNCC 2 cut(s) 55, 298
AsuC2I CCSGG 1 cut(s) 706
AsuHPI GGTGA 2 cut(s) 151, 705
AsuII TTCGAA 1 cut(s) 173
AvaI CYCGRG 1 cut(s) 302
AvaII GGWCC 1 cut(s) 55
BaeGI GKGCMC 2 cut(s) 220, 611
BalI TGGCCA 1 cut(s) 134
BbsI GAAGAC 2 cut(s) 423, 886
BbvI GCAGC 6 cut(s) 249, 319, 322, 366, 451, 547
BccI CCATC 2 cut(s) 85, 859
BciT130I CCWGG 2 cut(s) 136, 569
BcnI CCSGG 1 cut(s) 706
BcoDI GTCTC 2 cut(s) 23, 365
BfaI CTAG 2 cut(s) 674, 701
BfmI CTRYAG 2 cut(s) 263, 561
BfoI RGCGCY 1 cut(s) 263
BisI GCNGC 6 cut(s) 263, 308, 311, 355, 440, 561
BlsI GCNGC 6 cut(s) 264, 309, 312, 356, 441, 562
Bme1390I CCNGG 3 cut(s) 136, 569, 706
Bme18I GGWCC 1 cut(s) 55
BmeT110I CYCGRG 1 cut(s) 302
BmgBI CACGTC 1 cut(s) 375
BmgT120I GGNCC 2 cut(s) 55, 298
BmrFI CCNGG 3 cut(s) 136, 569, 706
BmrI ACTGGG 1 cut(s) 124
BmsI GCATC 1 cut(s) 807
BmuI ACTGGG 1 cut(s) 124
BpiI GAAGAC 2 cut(s) 423, 886
BpmI CTGGAG 1 cut(s) 389
Bpu14I TTCGAA 1 cut(s) 173
BpuMI CCSGG 1 cut(s) 706
BsaHI GRCGYC 1 cut(s) 678
BsaJI CCNNGG 4 cut(s) 135, 301, 705, 906
BsaWI WCCGGW 1 cut(s) 858
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 141, 706
Bse1I ACTGG 2 cut(s) 119, 847
Bse3DI GCAATG 2 cut(s) 27, 364
BseBI CCWGG 2 cut(s) 136, 569
BseDI CCNNGG 4 cut(s) 135, 301, 705, 906
BseLI CCNNNNNNNGG 3 cut(s) 91, 141, 706
BseMI GCAATG 2 cut(s) 27, 364
BseNI ACTGG 2 cut(s) 119, 847
BseRI GAGGAG 1 cut(s) 41
BseSI GKGCMC 2 cut(s) 220, 611
BseXI GCAGC 6 cut(s) 249, 319, 322, 366, 451, 547
BsgI GTGCAG 1 cut(s) 530
BshFI GGCC 4 cut(s) 134, 299, 379, 699
BsiHKCI CYCGRG 1 cut(s) 302
BsiSI CCGG 2 cut(s) 705, 859
BslI CCNNNNNNNGG 3 cut(s) 91, 141, 706
BsmAI GTCTC 2 cut(s) 23, 365
BsmBI CGTCTC 1 cut(s) 365
BsnI GGCC 4 cut(s) 134, 299, 379, 699
BsoBI CYCGRG 1 cut(s) 302
Bsp119I TTCGAA 1 cut(s) 173
Bsp1286I GDGCHC 2 cut(s) 220, 611
Bsp143I GATC 4 cut(s) 267, 364, 591, 626
BspANI GGCC 4 cut(s) 134, 299, 379, 699
BspMAI CTGCAG 2 cut(s) 267, 565
BspQI GCTCTTC 1 cut(s) 453
BspT104I TTCGAA 1 cut(s) 173
BsrDI GCAATG 2 cut(s) 27, 364
BsrI ACTGG 2 cut(s) 119, 847
BssECI CCNNGG 4 cut(s) 135, 301, 705, 906
BssMI GATC 4 cut(s) 267, 364, 591, 626
BssNI GRCGYC 1 cut(s) 678
BssT1I CCWWGG 1 cut(s) 906
Bst2UI CCWGG 2 cut(s) 136, 569
Bst6I CTCTTC 2 cut(s) 453, 754
BstACI GRCGYC 1 cut(s) 678
BstBI TTCGAA 1 cut(s) 173
BstC8I GCNNGC 1 cut(s) 324
BstH2I RGCGCY 1 cut(s) 263
BstHHI GCGC 1 cut(s) 262
BstKTI GATC 4 cut(s) 270, 367, 594, 629
BstMAI GTCTC 2 cut(s) 23, 365
BstMBI GATC 4 cut(s) 267, 364, 591, 626
BstMWI GCNNNNNNNGC 3 cut(s) 319, 569, 622
BstNI CCWGG 2 cut(s) 136, 569
BstSCI CCNGG 3 cut(s) 134, 567, 704
BstSFI CTRYAG 2 cut(s) 263, 561
BstSLI GKGCMC 2 cut(s) 220, 611
BstV1I GCAGC 6 cut(s) 249, 319, 322, 366, 451, 547
BstV2I GAAGAC 2 cut(s) 423, 886
BsuRI GGCC 4 cut(s) 134, 299, 379, 699
BtrI CACGTC 1 cut(s) 375
Cac8I GCNNGC 1 cut(s) 324
CaiI CAGNNNCTG 1 cut(s) 58
CfoI GCGC 1 cut(s) 262
Cfr13I GGNCC 2 cut(s) 55, 298
CviAII CATG 3 cut(s) 130, 580, 733
DpnI GATC 4 cut(s) 269, 366, 593, 628
DpnII GATC 4 cut(s) 267, 364, 591, 626
EaeI YGGCCR 1 cut(s) 132
Eam1104I CTCTTC 2 cut(s) 453, 754
EarI CTCTTC 2 cut(s) 453, 754
Eco130I CCWWGG 1 cut(s) 906
Eco47I GGWCC 1 cut(s) 55
Eco47III AGCGCT 1 cut(s) 261
Eco88I CYCGRG 1 cut(s) 302
EcoO109I RGGNCCY 1 cut(s) 55
EcoRI GAATTC 2 cut(s) 638, 846
EcoRII CCWGG 2 cut(s) 134, 567
EcoT14I CCWWGG 1 cut(s) 906
EcoT22I ATGCAT 1 cut(s) 766
ErhI CCWWGG 1 cut(s) 906
Esp3I CGTCTC 1 cut(s) 365
FaeI CATG 3 cut(s) 133, 583, 736
FatI CATG 3 cut(s) 129, 579, 732
FauNDI CATATG 1 cut(s) 208
Fnu4HI GCNGC 6 cut(s) 263, 308, 311, 355, 440, 561
Fsp4HI GCNGC 6 cut(s) 263, 308, 311, 355, 440, 561
FspBI CTAG 2 cut(s) 674, 701
GlaI GCGC 1 cut(s) 261
GluI GCNGC 6 cut(s) 263, 308, 311, 355, 440, 561
GsuI CTGGAG 1 cut(s) 389
HaeII RGCGCY 1 cut(s) 263
HaeIII GGCC 4 cut(s) 134, 299, 379, 699
HapII CCGG 2 cut(s) 705, 859
HhaI GCGC 1 cut(s) 262
Hin1I GRCGYC 1 cut(s) 678
Hin1II CATG 3 cut(s) 133, 583, 736
Hin6I GCGC 1 cut(s) 260
HinP1I GCGC 1 cut(s) 260
HindIII AAGCTT 2 cut(s) 5, 804
HinfI GANTC 3 cut(s) 167, 179, 236
HpaII CCGG 2 cut(s) 705, 859
HphI GGTGA 2 cut(s) 151, 705
Hpy166II GTNNAC 1 cut(s) 127
Hpy188I TCNGA 2 cut(s) 16, 591
Hpy188III TCNNGA 4 cut(s) 240, 368, 674, 891
Hpy8I GTNNAC 1 cut(s) 127
Hpy99I CGWCG 1 cut(s) 683
HpyAV CCTTC 4 cut(s) 237, 281, 390, 764
HpyCH4IV ACGT 3 cut(s) 374, 543, 678
HpyCH4V TGCA 8 cut(s) 206, 265, 347, 439, 511, 563, 764, 820
HpyF10VI GCNNNNNNNGC 3 cut(s) 319, 569, 622
HpySE526I ACGT 3 cut(s) 374, 543, 678
Hsp92I GRCGYC 1 cut(s) 678
Hsp92II CATG 3 cut(s) 133, 583, 736
HspAI GCGC 1 cut(s) 260
Kzo9I GATC 4 cut(s) 267, 364, 591, 626
LguI GCTCTTC 1 cut(s) 453
LmnI GCTCC 1 cut(s) 95
Lsp1109I GCAGC 6 cut(s) 249, 319, 322, 366, 451, 547
LweI GCATC 1 cut(s) 807
MaeI CTAG 2 cut(s) 674, 701
MaeII ACGT 3 cut(s) 374, 543, 678
MalI GATC 4 cut(s) 269, 366, 593, 628
MboI GATC 4 cut(s) 267, 364, 591, 626
MboII GAAGA 9 cut(s) 56, 157, 162, 176, 266, 428, 470, 741, 891
MhlI GDGCHC 2 cut(s) 220, 611
MlsI TGGCCA 1 cut(s) 134
MluCI AATT 8 cut(s) 348, 452, 531, 638, 692, 786, 846, 894
MluNI TGGCCA 1 cut(s) 134
MnlI CCTC 6 cut(s) 10, 19, 135, 311, 382, 442
Mox20I TGGCCA 1 cut(s) 134
Mph1103I ATGCAT 1 cut(s) 766
MroXI GAANNNNTTC 1 cut(s) 898
MscI TGGCCA 1 cut(s) 134
MseI TTAA 1 cut(s) 63
Msp20I TGGCCA 1 cut(s) 134
MspA1I CMGCKG 1 cut(s) 442
MspI CCGG 2 cut(s) 705, 859
MspR9I CCNGG 3 cut(s) 136, 569, 706
MvaI CCWGG 2 cut(s) 136, 569
MwoI GCNNNNNNNGC 3 cut(s) 319, 569, 622
NciI CCSGG 1 cut(s) 706
NdeI CATATG 1 cut(s) 208
NdeII GATC 4 cut(s) 267, 364, 591, 626
NlaIII CATG 3 cut(s) 133, 583, 736
NsiI ATGCAT 1 cut(s) 766
NspV TTCGAA 1 cut(s) 173
PciSI GCTCTTC 1 cut(s) 453
PdmI GAANNNNTTC 1 cut(s) 898
PfeI GAWTC 3 cut(s) 167, 179, 236
PflMI CCANNNNNTGG 1 cut(s) 91
PkrI GCNGC 6 cut(s) 264, 309, 312, 356, 441, 562
PpuMI RGGWCCY 1 cut(s) 55
PsiI TTATAA 1 cut(s) 405
Psp5II RGGWCCY 1 cut(s) 55
Psp6I CCWGG 2 cut(s) 134, 567
PspGI CCWGG 2 cut(s) 134, 567
PspPI GGNCC 2 cut(s) 55, 298
PspPPI RGGWCCY 1 cut(s) 55
PstI CTGCAG 2 cut(s) 267, 565
PstNI CAGNNNCTG 1 cut(s) 58
PvuII CAGCTG 1 cut(s) 442
SapI GCTCTTC 1 cut(s) 453
SaqAI TTAA 1 cut(s) 63
SatI GCNGC 6 cut(s) 263, 308, 311, 355, 440, 561
Sau3AI GATC 4 cut(s) 267, 364, 591, 626
Sau96I GGNCC 2 cut(s) 55, 298
ScrFI CCNGG 3 cut(s) 136, 569, 706
SduI GDGCHC 2 cut(s) 220, 611
SfaNI GCATC 1 cut(s) 807
SfcI CTRYAG 2 cut(s) 263, 561
SfuI TTCGAA 1 cut(s) 173
SinI GGWCC 1 cut(s) 55
Sse9I AATT 8 cut(s) 348, 452, 531, 638, 692, 786, 846, 894
SspMI CTAG 2 cut(s) 674, 701
StyD4I CCNGG 3 cut(s) 134, 567, 704
StyI CCWWGG 1 cut(s) 906
TaiI ACGT 3 cut(s) 377, 546, 681
TaqI TCGA 2 cut(s) 173, 447
TasI AATT 8 cut(s) 348, 452, 531, 638, 692, 786, 846, 894
TfiI GAWTC 3 cut(s) 167, 179, 236
Tru1I TTAA 1 cut(s) 63
Tru9I TTAA 1 cut(s) 63
TseI GCWGC 6 cut(s) 262, 307, 310, 354, 439, 560
TspDTI ATGAA 4 cut(s) 17, 300, 570, 681
Van91I CCANNNNNTGG 1 cut(s) 91
VpaK11BI GGWCC 1 cut(s) 55
XapI RAATTY 5 cut(s) 531, 638, 692, 846, 894
XbaI TCTAGA 1 cut(s) 673
XmnI GAANNNNTTC 1 cut(s) 898
XspI CTAG 2 cut(s) 674, 701
ZraI GACGTC 1 cut(s) 679
Zsp2I ATGCAT 1 cut(s) 766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.