Rw2G002020

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0029208
Physical Location & Seq
Forward (+)
1681351 .. 1683723
2373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G002020.1

Sequence Viewer

Length: 1584 bp
ATGAAGGGAAGAAAGTTCATGAGGACAATACGGGGGGGAATACTGAAGACAAGATTGAGGAAGAAGGCTGTGGAAATAGGTGAGCAAAAGAAGTCTGGGGGTATAGGTGAGGTAGATAAGGATCTGGAAATAGCTGAGCAAAAGAAGGCAGAGGTCAAGGTAGTTGTGGAGGAGAAGATCACAACAACAGAAGAAGTGCTGAAAAGAAGTGTGAAATTGGTGTTGGGTGAGGACATAAGATGGGCTCAGTTGCCAGTAAATTGCACCCTCCTGCAACTAAGAGAGGTGATCCGTGATCGGTTTCCAAGATCAAGAGCAGTTCTTATTAAATATAGAGACGAAGAAGGTGATTTGGTCACCATCACTACTAATGAAGAGCTGAGATGGGCTGAAGAATCTGCAGAATCAGAAGGCTCTGTCAGGCTTTACATAATAGAAGTTAGCCCTGATCAGGATCCATTCTTTCAGGAACTGAAAGTTGAACCGCACAAGCTTAACTATGGAGCTGAGAATGGTACATTGGTACACCACAAGGACATGAAGGGGTCATATTGCATTGAGGATTGGATTATTGATTTTGCTCAGCTGTTCAAGAACTATGCTGGCTTTGAATCTGATGCATACTTGGATCTTCATGAACTTGGGATGAAGTTCTACTCTGAGGCTATGGAGGAGACAGTTACCAGTGAAGAAGCTCAAGACATTTTCGACACAGCAGGAGAGAAGTTCCAGGAAATGGGAGCTTTAGCGTTGTTCAATTGGGGAAATGTTCACGTGGCCAGGGCAAGGAAGAAGGTATATTTCACAGAAGATTCTTCCAAAGAATCTATCATTTCAAACATCAAAACTGCATATGAATGGGCACAAAAAGAATACACAGAAGCTGGAAGGAGATATGAAGAAGCGCTGCATATCAAACCCGACTTCTATGAAGGCTATCTGGCCCTTGGGCAGCAGCAGTTTGAGCAGGCTAAACTTTCTTGGTATTATGCAATTAGCAGCAATGTTGATTTGGAGACTTGGCCATCCACAGAGGTTCTAAGACTTTATAACAAGGCCGAAGACAACATGGAAAAGGGCATGCAGCTGTGGGAGGAATCGGAAGAGCAACGGTTGAGTGAACTCTCTAGTCCAACTAGTTGTAAAACCCAGTTGCAGAAGATGGGTTTGGATGGGATTGTCAAAGACATATCAGCAGATGAAGCTGCAGAACAAGCTACAAACATGAGATCTCAGATAAACCTTCTTTGGGGCACCATGCTGTACGAGCGATCTATAGTGGAGTTCAAATTGGGGCTACCTGTATGGCATGAATGTTTGGAAGTTGCAGTTGAGAAGTTTGGACTTGCTGGGGCTTCTCCAACAGATATAGCTGTTATGGTCAAAAACCACTCTTCAAATGATAATGCATTGGAAGGTTTAGGATTCAAGATTGATGAGATAATACAAGCATGGAATGAGATGTATGAAGCTAAGAAGTGGCAAAGTGGAATTCCATCATTTCGGCTAGAGCCATTACTTCGGCGGAGAGTTTCAAAACTTCATTTTGCTTTGGAGAATGCTTTAAAAGACAGCACTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

527

Amino Acids

60.54

Weight (kDa)

5.08

Isoelectric Point (pI)

48.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 70 - 144 5.8e-14 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029208)

Species Orthologous Gene IDs
pyrus_communis pycom02g01650
rosa_wichuraiana Rw2G002020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1050
AccB1I GGYRCC 1 cut(s) 1253
AccB7I CCANNNNNTGG 1 cut(s) 736
AciI CCGC 2 cut(s) 485, 1525
AclWI GGATC 5 cut(s) 129, 283, 449, 462, 636
AcoI YGGCCR 2 cut(s) 777, 1022
AcsI RAATTY 1 cut(s) 1491
AcuI CTGAAG 2 cut(s) 65, 411
AcvI CACGTG 1 cut(s) 775
AfaI GTAC 3 cut(s) 517, 525, 1265
AfeI AGCGCT 1 cut(s) 906
AfiI CCNNNNNNNGG 4 cut(s) 451, 736, 786, 1249
AgsI TTSAA 9 cut(s) 482, 592, 611, 757, 837, 1288, 1398, 1429, 1536
AhlI ACTAGT 1 cut(s) 1136
AjnI CCWGG 2 cut(s) 729, 779
AjuI GAANNNNNNNTTGG 6 cut(s) 206, 238, 503, 535, 1151, 1183
Alw26I GTCTC 3 cut(s) 330, 668, 1010
AlwI GGATC 5 cut(s) 129, 283, 449, 462, 636
AlwNI CAGNNNCTG 2 cut(s) 472, 884
Aor51HI AGCGCT 1 cut(s) 906
AoxI GGCC 4 cut(s) 777, 942, 1022, 1056
ApeKI GCWGC 6 cut(s) 907, 952, 955, 999, 1084, 1205
ApoI RAATTY 1 cut(s) 1491
AspLEI GCGC 1 cut(s) 907
AspS9I GGNCC 1 cut(s) 943
AsuHPI GGTGA 6 cut(s) 92, 119, 239, 298, 349, 359
BaeGI GKGCMC 2 cut(s) 865, 1256
BaeI ACNNNNGTAYC 2 cut(s) 507, 540
BalI TGGCCA 2 cut(s) 779, 1024
BamHI GGATCC 1 cut(s) 454
BanI GGYRCC 1 cut(s) 1253
BanII GRGCYC 1 cut(s) 247
BbrPI CACGTG 1 cut(s) 775
BbsI GAAGAC 2 cut(s) 53, 1068
BbvI GCAGC 6 cut(s) 894, 964, 967, 1011, 1096, 1192
BccI CCATC 7 cut(s) 234, 368, 378, 1033, 1156, 1166, 1504
BciT130I CCWGG 2 cut(s) 731, 781
BclI TGATCA 1 cut(s) 448
BcoDI GTCTC 3 cut(s) 330, 668, 1010
BcuI ACTAGT 1 cut(s) 1136
BfaI CTAG 3 cut(s) 1128, 1137, 1508
BfmI CTRYAG 3 cut(s) 399, 1206, 1275
BfoI RGCGCY 1 cut(s) 908
BglII AGATCT 1 cut(s) 1229
BisI GCNGC 6 cut(s) 908, 953, 956, 1000, 1085, 1206
BlpI GCTNAGC 2 cut(s) 135, 582
BlsI GCNGC 6 cut(s) 909, 954, 957, 1001, 1086, 1207
Bme1390I CCNGG 2 cut(s) 731, 781
BmgT120I GGNCC 1 cut(s) 943
BmiI GGNNCC 2 cut(s) 456, 1255
BmrFI CCNGG 2 cut(s) 731, 781
BmrI ACTGGG 1 cut(s) 1144
BmsI GCATC 1 cut(s) 607
BmuI ACTGGG 1 cut(s) 1144
BpiI GAAGAC 2 cut(s) 53, 1068
Bpu1102I GCTNAGC 2 cut(s) 135, 582
BpuEI CTTGAG 1 cut(s) 681
BsaAI YACGTR 1 cut(s) 775
BsaBI GATNNNNATC 2 cut(s) 120, 453
BsaJI CCNNGG 2 cut(s) 780, 946
Bsc4I CCNNNNNNNGG 4 cut(s) 451, 736, 786, 1249
Bse1I ACTGG 3 cut(s) 254, 684, 1150
Bse3DI GCAATG 1 cut(s) 1009
Bse8I GATNNNNATC 2 cut(s) 120, 453
BseBI CCWGG 2 cut(s) 731, 781
BseDI CCNNGG 2 cut(s) 780, 946
BseGI GGATG 3 cut(s) 651, 1025, 1177
BseJI GATNNNNATC 2 cut(s) 120, 453
BseLI CCNNNNNNNGG 4 cut(s) 451, 736, 786, 1249
BseMI GCAATG 1 cut(s) 1009
BseMII CTCAG 7 cut(s) 126, 260, 371, 498, 596, 651, 1247
BseNI ACTGG 3 cut(s) 254, 684, 1150
BseRI GAGGAG 2 cut(s) 185, 686
BseSI GKGCMC 2 cut(s) 865, 1256
BseXI GCAGC 6 cut(s) 894, 964, 967, 1011, 1096, 1192
BseYI CCCAGC 1 cut(s) 1349
BshFI GGCC 4 cut(s) 779, 944, 1024, 1058
BshNI GGYRCC 1 cut(s) 1253
BslI CCNNNNNNNGG 4 cut(s) 451, 736, 786, 1249
BsmAI GTCTC 3 cut(s) 330, 668, 1010
BsmBI CGTCTC 1 cut(s) 330
BsmI GAATGC 1 cut(s) 1564
BsnI GGCC 4 cut(s) 779, 944, 1024, 1058
Bsp1286I GDGCHC 3 cut(s) 247, 865, 1256
Bsp1720I GCTNAGC 2 cut(s) 135, 582
BspACI CCGC 2 cut(s) 485, 1525
BspANI GGCC 4 cut(s) 779, 944, 1024, 1058
BspCNI CTCAG 7 cut(s) 127, 259, 372, 499, 595, 652, 1246
BspHI TCATGA 2 cut(s) 18, 634
BspLI GGNNCC 2 cut(s) 456, 1255
BspMAI CTGCAG 2 cut(s) 403, 1210
BspPI GGATC 5 cut(s) 129, 283, 449, 462, 636
BspQI GCTCTTC 2 cut(s) 369, 1098
BspT107I GGYRCC 1 cut(s) 1253
BsrDI GCAATG 1 cut(s) 1009
BsrI ACTGG 3 cut(s) 254, 684, 1150
BssECI CCNNGG 2 cut(s) 780, 946
BssT1I CCWWGG 1 cut(s) 946
Bst2UI CCWGG 2 cut(s) 731, 781
Bst4CI ACNGT 2 cut(s) 679, 1113
Bst6I CTCTTC 3 cut(s) 369, 1098, 1399
BstBAI YACGTR 1 cut(s) 775
BstC8I GCNNGC 3 cut(s) 604, 969, 1082
BstEII GGTNACC 1 cut(s) 355
BstF5I GGATG 3 cut(s) 651, 1025, 1177
BstH2I RGCGCY 1 cut(s) 908
BstHHI GCGC 1 cut(s) 907
BstMAI GTCTC 3 cut(s) 330, 668, 1010
BstMWI GCNNNNNNNGC 4 cut(s) 964, 1202, 1214, 1267
BstNI CCWGG 2 cut(s) 731, 781
BstNSI RCATGY 1 cut(s) 1084
BstPI GGTNACC 1 cut(s) 355
BstSCI CCNGG 2 cut(s) 729, 779
BstSFI CTRYAG 3 cut(s) 399, 1206, 1275
BstSLI GKGCMC 2 cut(s) 865, 1256
BstV1I GCAGC 6 cut(s) 894, 964, 967, 1011, 1096, 1192
BstV2I GAAGAC 2 cut(s) 53, 1068
BstX2I RGATCY 4 cut(s) 121, 454, 628, 1229
BstYI RGATCY 4 cut(s) 121, 454, 628, 1229
BsuRI GGCC 4 cut(s) 779, 944, 1024, 1058
BtsCI GGATG 3 cut(s) 651, 1025, 1177
BtsIMutI CAGTG 2 cut(s) 691, 1575
Cac8I GCNNGC 3 cut(s) 604, 969, 1082
CaiI CAGNNNCTG 2 cut(s) 472, 884
CciI TCATGA 2 cut(s) 18, 634
CfoI GCGC 1 cut(s) 907
Cfr13I GGNCC 1 cut(s) 943
Csp6I GTAC 3 cut(s) 516, 524, 1264
CviAII CATG 9 cut(s) 19, 538, 635, 1069, 1081, 1225, 1258, 1310, 1452
CviQI GTAC 3 cut(s) 516, 524, 1264
DraI TTTAAA 1 cut(s) 1566
EaeI YGGCCR 2 cut(s) 777, 1022
Eam1104I CTCTTC 3 cut(s) 369, 1098, 1399
EarI CTCTTC 3 cut(s) 369, 1098, 1399
EciI GGCGGA 1 cut(s) 1540
Eco130I CCWWGG 1 cut(s) 946
Eco24I GRGCYC 1 cut(s) 247
Eco47III AGCGCT 1 cut(s) 906
Eco57I CTGAAG 2 cut(s) 65, 411
Eco72I CACGTG 1 cut(s) 775
Eco91I GGTNACC 1 cut(s) 355
EcoO65I GGTNACC 1 cut(s) 355
EcoRI GAATTC 1 cut(s) 1491
EcoRII CCWGG 2 cut(s) 729, 779
EcoT14I CCWWGG 1 cut(s) 946
EcoT22I ATGCAT 2 cut(s) 622, 1411
EcoT38I GRGCYC 1 cut(s) 247
ErhI CCWWGG 1 cut(s) 946
Esp3I CGTCTC 1 cut(s) 330
FaeI CATG 9 cut(s) 22, 541, 638, 1072, 1084, 1228, 1261, 1313, 1455
FatI CATG 9 cut(s) 18, 537, 634, 1068, 1080, 1224, 1257, 1309, 1451
FauNDI CATATG 1 cut(s) 853
FbaI TGATCA 1 cut(s) 448
Fnu4HI GCNGC 6 cut(s) 908, 953, 956, 1000, 1085, 1206
FokI GGATG 3 cut(s) 658, 1012, 1184
FriOI GRGCYC 1 cut(s) 247
Fsp4HI GCNGC 6 cut(s) 908, 953, 956, 1000, 1085, 1206
FspBI CTAG 3 cut(s) 1128, 1137, 1508
GlaI GCGC 1 cut(s) 906
GluI GCNGC 6 cut(s) 908, 953, 956, 1000, 1085, 1206
GsaI CCCAGC 1 cut(s) 1353
HaeII RGCGCY 1 cut(s) 908
HaeIII GGCC 4 cut(s) 779, 944, 1024, 1058
HhaI GCGC 1 cut(s) 907
Hin1II CATG 9 cut(s) 22, 541, 638, 1072, 1084, 1228, 1261, 1313, 1455
Hin6I GCGC 1 cut(s) 905
HinP1I GCGC 1 cut(s) 905
HindIII AAGCTT 1 cut(s) 491
HinfI GANTC 7 cut(s) 395, 404, 611, 812, 824, 1097, 1425
HphI GGTGA 6 cut(s) 92, 119, 239, 298, 349, 359
Hpy166II GTNNAC 3 cut(s) 526, 772, 1121
Hpy188I TCNGA 5 cut(s) 409, 616, 661, 1102, 1236
Hpy188III TCNNGA 9 cut(s) 19, 125, 312, 452, 467, 592, 635, 698, 1429
Hpy8I GTNNAC 3 cut(s) 526, 772, 1121
HpyCH4III ACNGT 2 cut(s) 679, 1113
HpyCH4IV ACGT 1 cut(s) 774
HpyF10VI GCNNNNNNNGC 4 cut(s) 964, 1202, 1214, 1267
HpySE526I ACGT 1 cut(s) 774
Hsp92II CATG 9 cut(s) 22, 541, 638, 1072, 1084, 1228, 1261, 1313, 1455
HspAI GCGC 1 cut(s) 905
Ksp22I TGATCA 1 cut(s) 448
LguI GCTCTTC 2 cut(s) 369, 1098
LmnI GCTCC 2 cut(s) 503, 740
Lsp1109I GCAGC 6 cut(s) 894, 964, 967, 1011, 1096, 1192
LweI GCATC 1 cut(s) 607
MaeI CTAG 3 cut(s) 1128, 1137, 1508
MaeII ACGT 1 cut(s) 774
MaeIII GTNAC 2 cut(s) 355, 679
MfeI CAATTG 1 cut(s) 757
MflI RGATCY 4 cut(s) 121, 454, 628, 1229
MhlI GDGCHC 3 cut(s) 247, 865, 1256
MlsI TGGCCA 2 cut(s) 779, 1024
MluCI AATT 6 cut(s) 215, 259, 757, 993, 1289, 1491
MluNI TGGCCA 2 cut(s) 779, 1024
MmeI TCCRAC 2 cut(s) 1157, 1385
Mox20I TGGCCA 2 cut(s) 779, 1024
Mph1103I ATGCAT 2 cut(s) 622, 1411
MscI TGGCCA 2 cut(s) 779, 1024
MseI TTAA 3 cut(s) 327, 495, 1565
MslI CAYNNNNRTG 1 cut(s) 856
Msp20I TGGCCA 2 cut(s) 779, 1024
MspA1I CMGCKG 2 cut(s) 586, 1087
MspR9I CCNGG 2 cut(s) 731, 781
MunI CAATTG 1 cut(s) 757
Mva1269I GAATGC 1 cut(s) 1564
MvaI CCWGG 2 cut(s) 731, 781
MwoI GCNNNNNNNGC 4 cut(s) 964, 1202, 1214, 1267
NdeI CATATG 1 cut(s) 853
NlaIII CATG 9 cut(s) 22, 541, 638, 1072, 1084, 1228, 1261, 1313, 1455
NlaIV GGNNCC 2 cut(s) 456, 1255
NmuCI GTSAC 1 cut(s) 355
NsiI ATGCAT 2 cut(s) 622, 1411
NspI RCATGY 1 cut(s) 1084
PaeI GCATGC 1 cut(s) 1084
PagI TCATGA 2 cut(s) 18, 634
PciSI GCTCTTC 2 cut(s) 369, 1098
PctI GAATGC 1 cut(s) 1564
PfeI GAWTC 7 cut(s) 395, 404, 611, 812, 824, 1097, 1425
PflMI CCANNNNNTGG 1 cut(s) 736
PfoI TCCNGGA 1 cut(s) 729
PkrI GCNGC 6 cut(s) 909, 954, 957, 1001, 1086, 1207
PmaCI CACGTG 1 cut(s) 775
PmlI CACGTG 1 cut(s) 775
Ppu21I YACGTR 1 cut(s) 775
PsiI TTATAA 1 cut(s) 1050
Psp6I CCWGG 2 cut(s) 729, 779
PspCI CACGTG 1 cut(s) 775
PspEI GGTNACC 1 cut(s) 355
PspFI CCCAGC 1 cut(s) 1349
PspGI CCWGG 2 cut(s) 729, 779
PspN4I GGNNCC 2 cut(s) 456, 1255
PspPI GGNCC 1 cut(s) 943
PstI CTGCAG 2 cut(s) 403, 1210
PstNI CAGNNNCTG 2 cut(s) 472, 884
PsuI RGATCY 4 cut(s) 121, 454, 628, 1229
PvuII CAGCTG 2 cut(s) 586, 1087
RsaI GTAC 3 cut(s) 517, 525, 1265
RsaNI GTAC 3 cut(s) 516, 524, 1264
RseI CAYNNNNRTG 1 cut(s) 856
SapI GCTCTTC 2 cut(s) 369, 1098
SaqAI TTAA 3 cut(s) 327, 495, 1565
SatI GCNGC 6 cut(s) 908, 953, 956, 1000, 1085, 1206
Sau96I GGNCC 1 cut(s) 943
ScrFI CCNGG 2 cut(s) 731, 781
SduI GDGCHC 3 cut(s) 247, 865, 1256
SfaNI GCATC 1 cut(s) 607
SfcI CTRYAG 3 cut(s) 399, 1206, 1275
SmiMI CAYNNNNRTG 1 cut(s) 856
SmlI CTYRAG 1 cut(s) 696
SmoI CTYRAG 1 cut(s) 696
SpeI ACTAGT 1 cut(s) 1136
SphI GCATGC 1 cut(s) 1084
Sse9I AATT 6 cut(s) 215, 259, 757, 993, 1289, 1491
SsiI CCGC 2 cut(s) 485, 1525
SspMI CTAG 3 cut(s) 1128, 1137, 1508
StyD4I CCNGG 2 cut(s) 729, 779
StyI CCWWGG 1 cut(s) 946
TaaI ACNGT 2 cut(s) 679, 1113
TaiI ACGT 1 cut(s) 777
TaqI TCGA 1 cut(s) 708
TasI AATT 6 cut(s) 215, 259, 757, 993, 1289, 1491
TfiI GAWTC 7 cut(s) 395, 404, 611, 812, 824, 1097, 1425
Tru1I TTAA 3 cut(s) 327, 495, 1565
Tru9I TTAA 3 cut(s) 327, 495, 1565
TscAI CASTG 2 cut(s) 691, 1582
TseFI GTSAC 1 cut(s) 355
TseI GCWGC 6 cut(s) 907, 952, 955, 999, 1084, 1205
Tsp45I GTSAC 1 cut(s) 355
TspGWI ACGGA 1 cut(s) 281
TspRI CASTG 2 cut(s) 691, 1582
Van91I CCANNNNNTGG 1 cut(s) 736
XapI RAATTY 1 cut(s) 1491
XceI RCATGY 1 cut(s) 1084
XspI CTAG 3 cut(s) 1128, 1137, 1508
Zsp2I ATGCAT 2 cut(s) 622, 1411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.