Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate
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| Species | Orthologous Gene IDs |
|---|---|
| malus_domestica | MD07G1089600.v1.1 |
| pyrus_communis | pycom02g18000 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 367 |
| AccII | CGCG | 2 cut(s) | 252, 261 |
| AciI | CCGC | 1 cut(s) | 259 |
| AcsI | RAATTY | 6 cut(s) | 12, 20, 54, 93, 419, 426 |
| AfaI | GTAC | 1 cut(s) | 309 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 317 |
| AgsI | TTSAA | 2 cut(s) | 185, 198 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 297, 329 |
| AluBI | AGCT | 2 cut(s) | 323, 374 |
| AluI | AGCT | 2 cut(s) | 323, 374 |
| Alw26I | GTCTC | 1 cut(s) | 353 |
| ApoI | RAATTY | 6 cut(s) | 12, 20, 54, 93, 419, 426 |
| AspS9I | GGNCC | 1 cut(s) | 152 |
| AsuII | TTCGAA | 1 cut(s) | 17 |
| AvaII | GGWCC | 1 cut(s) | 152 |
| BccI | CCATC | 1 cut(s) | 285 |
| BcoDI | GTCTC | 1 cut(s) | 353 |
| Bme18I | GGWCC | 1 cut(s) | 152 |
| BmgT120I | GGNCC | 1 cut(s) | 152 |
| BpmI | CTGGAG | 1 cut(s) | 360 |
| Bpu14I | TTCGAA | 1 cut(s) | 17 |
| BsaI | GGTCTC | 1 cut(s) | 353 |
| BsaWI | WCCGGW | 1 cut(s) | 149 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 258, 288 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 317 |
| Bse1I | ACTGG | 1 cut(s) | 343 |
| BseGI | GGATG | 1 cut(s) | 277 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 317 |
| BseMII | CTCAG | 2 cut(s) | 235, 296 |
| BseNI | ACTGG | 1 cut(s) | 343 |
| Bsh1236I | CGCG | 2 cut(s) | 252, 261 |
| BsiSI | CCGG | 1 cut(s) | 150 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 317 |
| BsmAI | GTCTC | 1 cut(s) | 353 |
| Bso31I | GGTCTC | 1 cut(s) | 353 |
| Bsp119I | TTCGAA | 1 cut(s) | 17 |
| BspACI | CCGC | 1 cut(s) | 259 |
| BspCNI | CTCAG | 2 cut(s) | 234, 295 |
| BspFNI | CGCG | 2 cut(s) | 252, 261 |
| BspT104I | TTCGAA | 1 cut(s) | 17 |
| BspTNI | GGTCTC | 1 cut(s) | 353 |
| BsrI | ACTGG | 1 cut(s) | 343 |
| Bst4CI | ACNGT | 3 cut(s) | 8, 108, 119 |
| Bst6I | CTCTTC | 1 cut(s) | 141 |
| BstBI | TTCGAA | 1 cut(s) | 17 |
| BstC8I | GCNNGC | 1 cut(s) | 325 |
| BstDEI | CTNAG | 4 cut(s) | 221, 282, 328, 375 |
| BstF5I | GGATG | 1 cut(s) | 277 |
| BstFNI | CGCG | 2 cut(s) | 252, 261 |
| BstMAI | GTCTC | 1 cut(s) | 353 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 258 |
| BstNSI | RCATGY | 1 cut(s) | 73 |
| BstUI | CGCG | 2 cut(s) | 252, 261 |
| BtsCI | GGATG | 1 cut(s) | 277 |
| Cac8I | GCNNGC | 1 cut(s) | 325 |
| Cfr13I | GGNCC | 1 cut(s) | 152 |
| CseI | GACGC | 2 cut(s) | 272, 395 |
| Csp6I | GTAC | 1 cut(s) | 308 |
| CviAII | CATG | 2 cut(s) | 62, 70 |
| CviJI | RGCY | 5 cut(s) | 170, 216, 323, 327, 374 |
| CviKI_1 | RGCY | 5 cut(s) | 170, 216, 323, 327, 374 |
| CviQI | GTAC | 1 cut(s) | 308 |
| DdeI | CTNAG | 4 cut(s) | 221, 282, 328, 375 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 367 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 367 |
| Eam1104I | CTCTTC | 1 cut(s) | 141 |
| EarI | CTCTTC | 1 cut(s) | 141 |
| Eco31I | GGTCTC | 1 cut(s) | 353 |
| Eco47I | GGWCC | 1 cut(s) | 152 |
| EcoRI | GAATTC | 1 cut(s) | 93 |
| FaeI | CATG | 2 cut(s) | 65, 73 |
| FaiI | YATR | 3 cut(s) | 63, 71, 300 |
| FatI | CATG | 2 cut(s) | 61, 69 |
| FokI | GGATG | 1 cut(s) | 264 |
| GsuI | CTGGAG | 1 cut(s) | 360 |
| HapII | CCGG | 1 cut(s) | 150 |
| HgaI | GACGC | 2 cut(s) | 272, 395 |
| Hin1II | CATG | 2 cut(s) | 65, 73 |
| HinfI | GANTC | 2 cut(s) | 127, 191 |
| HpaII | CCGG | 1 cut(s) | 150 |
| Hpy166II | GTNNAC | 1 cut(s) | 246 |
| Hpy188I | TCNGA | 1 cut(s) | 285 |
| Hpy188III | TCNNGA | 1 cut(s) | 401 |
| Hpy8I | GTNNAC | 1 cut(s) | 246 |
| Hpy99I | CGWCG | 3 cut(s) | 123, 266, 389 |
| HpyAV | CCTTC | 3 cut(s) | 37, 150, 181 |
| HpyCH4III | ACNGT | 3 cut(s) | 8, 108, 119 |
| HpyCH4V | TGCA | 1 cut(s) | 4 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 258 |
| HpyF3I | CTNAG | 4 cut(s) | 221, 282, 328, 375 |
| Hsp92II | CATG | 2 cut(s) | 65, 73 |
| LmnI | GCTCC | 2 cut(s) | 152, 320 |
| LpnPI | CCDG | 6 cut(s) | 157, 163, 254, 309, 324, 366 |
| MboII | GAAGA | 4 cut(s) | 128, 186, 314, 389 |
| MluCI | AATT | 8 cut(s) | 12, 20, 54, 93, 227, 390, 419, 426 |
| MnlI | CCTC | 4 cut(s) | 144, 165, 185, 291 |
| MseI | TTAA | 1 cut(s) | 294 |
| MspI | CCGG | 1 cut(s) | 150 |
| MvnI | CGCG | 2 cut(s) | 252, 261 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 258 |
| NlaIII | CATG | 2 cut(s) | 65, 73 |
| NspI | RCATGY | 1 cut(s) | 73 |
| NspV | TTCGAA | 1 cut(s) | 17 |
| PfeI | GAWTC | 2 cut(s) | 127, 191 |
| PspPI | GGNCC | 1 cut(s) | 152 |
| RsaI | GTAC | 1 cut(s) | 309 |
| RsaNI | GTAC | 1 cut(s) | 308 |
| SaqAI | TTAA | 1 cut(s) | 294 |
| Sau96I | GGNCC | 1 cut(s) | 152 |
| SetI | ASST | 6 cut(s) | 136, 243, 313, 325, 355, 376 |
| SfuI | TTCGAA | 1 cut(s) | 17 |
| SinI | GGWCC | 1 cut(s) | 152 |
| Sse9I | AATT | 8 cut(s) | 12, 20, 54, 93, 227, 390, 419, 426 |
| SsiI | CCGC | 1 cut(s) | 259 |
| TaaI | ACNGT | 3 cut(s) | 8, 108, 119 |
| TaqI | TCGA | 8 cut(s) | 17, 46, 97, 103, 121, 164, 384, 402 |
| TasI | AATT | 8 cut(s) | 12, 20, 54, 93, 227, 390, 419, 426 |
| TfiI | GAWTC | 2 cut(s) | 127, 191 |
| Tru1I | TTAA | 1 cut(s) | 294 |
| Tru9I | TTAA | 1 cut(s) | 294 |
| TspDTI | ATGAA | 2 cut(s) | 32, 315 |
| VpaK11BI | GGWCC | 1 cut(s) | 152 |
| XapI | RAATTY | 6 cut(s) | 12, 20, 54, 93, 419, 426 |
| XceI | RCATGY | 1 cut(s) | 73 |