pycom02g24450

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0029196
Physical Location & Seq
Forward (+)
22621939 .. 22622821
883 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 717 bp
ATGAGCTTCCTAGCTCTCCATGAAGAAAAGCATTCTTTACATCCATCTGCCACAAATTCCAGTCTTTGTTGGCTGCAAGTGCAAGTAAGACTCAGTCTAGTCCGTACTGTTGAGAGAAACCACGAGCTACCAATCGTGCCTTGTACCTCTCGATTGACCCATCTGGACGACGCTTTATCTTGTAAACCCACTTGCAGGATATGGGTTTCACATCTCTTGACTTTGGCACGAGATCCCAAGTCTGATTTTGCTGAAGTGCATCAAGCTTCTTCGAACGTCTCAGGTTCTGTTGCAGTTGTTTCTTCAATTATGGCTGCATTGGTGTATTTGGGATTTGGCCTTCGTGTTCTTGTTGACCTCCTTAGTTGTGATCGTGGAGTTGATACTTCCACTTCACTAGGGTTTTGGGGCATTGCTTGCTTAACATCATCACCATCAAGCAAATCTTCAGACTCATCAGGTCTTGACTGGATTCGAACAATTTGCTCCCCTATCTTTTGCTGCAACTTATCTTCTATCTCTCTTGAATCAGGCAACGCCTCCTTTTCTGAGCACCACCAAGAAGATGCTTCAGAATCCCATTCTTCATCCATAGCTAGATATGCTATGGCATTCCAATCATCTTCACTTTTCTTCTCTAAGTTGACAACATTACTTTCTGCAGGCCTTTTGAACCAGCAATCCTTCGCCATGTGGTCATTCTTTCCACAATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0002376 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031410 GO:0031974 GO:0031981 GO:0031982 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0040007 GO:0042742 GO:0043086 GO:0043087 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0051704 GO:0051707 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0097708 GO:0098542 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

239

Amino Acids

25.94

Weight (kDa)

6.17

Isoelectric Point (pI)

47.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029196)

Species Orthologous Gene IDs
pyrus_communis pycom01g12070 pycom02g24450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 227
AcsI RAATTY 1 cut(s) 55
AcuI CTGAAG 3 cut(s) 273, 432, 555
AfaI GTAC 2 cut(s) 106, 145
AfiI CCNNNNNNNGG 1 cut(s) 195
AgsI TTSAA 3 cut(s) 306, 527, 673
AloI GAACNNNNNNTCC 2 cut(s) 665, 697
AluBI AGCT 5 cut(s) 6, 14, 127, 266, 596
AluI AGCT 5 cut(s) 6, 14, 127, 266, 596
Alw21I GWGCWC 1 cut(s) 555
Alw26I GTCTC 1 cut(s) 283
AlwI GGATC 1 cut(s) 227
AlwNI CAGNNNCTG 1 cut(s) 287
AoxI GGCC 2 cut(s) 337, 664
ApeKI GCWGC 3 cut(s) 73, 314, 501
ApoI RAATTY 1 cut(s) 55
AsuHPI GGTGA 1 cut(s) 423
AsuII TTCGAA 2 cut(s) 272, 475
BauI CACGAG 2 cut(s) 122, 228
Bbv12I GWGCWC 1 cut(s) 555
BbvI GCAGC 3 cut(s) 60, 301, 488
BccI CCATC 3 cut(s) 52, 168, 442
BcgI CGANNNNNNTGC 2 cut(s) 465, 499
BcoDI GTCTC 1 cut(s) 283
BfaI CTAG 4 cut(s) 11, 98, 398, 597
BfmI CTRYAG 1 cut(s) 660
BisI GCNGC 3 cut(s) 74, 315, 502
BlsI GCNGC 3 cut(s) 75, 316, 503
BmsI GCATC 2 cut(s) 268, 556
Bpu14I TTCGAA 2 cut(s) 272, 475
Bsc4I CCNNNNNNNGG 1 cut(s) 195
Bse1I ACTGG 2 cut(s) 60, 473
Bse3DI GCAATG 1 cut(s) 411
BseGI GGATG 2 cut(s) 40, 587
BseLI CCNNNNNNNGG 1 cut(s) 195
BseMI GCAATG 1 cut(s) 411
BseMII CTCAG 3 cut(s) 106, 294, 540
BseNI ACTGG 2 cut(s) 60, 473
BseXI GCAGC 3 cut(s) 60, 301, 488
BshFI GGCC 2 cut(s) 339, 666
BsiHKAI GWGCWC 1 cut(s) 555
BslI CCNNNNNNNGG 1 cut(s) 195
BsmAI GTCTC 1 cut(s) 283
BsmBI CGTCTC 1 cut(s) 283
BsmI GAATGC 2 cut(s) 31, 611
BsnI GGCC 2 cut(s) 339, 666
Bsp119I TTCGAA 2 cut(s) 272, 475
Bsp1286I GDGCHC 1 cut(s) 555
Bsp143I GATC 2 cut(s) 232, 370
BspANI GGCC 2 cut(s) 339, 666
BspCNI CTCAG 3 cut(s) 105, 293, 541
BspMAI CTGCAG 1 cut(s) 664
BspPI GGATC 1 cut(s) 227
BspT104I TTCGAA 2 cut(s) 272, 475
BsrDI GCAATG 1 cut(s) 411
BsrI ACTGG 2 cut(s) 60, 473
BssMI GATC 2 cut(s) 232, 370
BssSI CACGAG 2 cut(s) 122, 228
Bst2BI CACGAG 2 cut(s) 122, 228
Bst4CI ACNGT 1 cut(s) 109
BstAPI GCANNNNNTGC 1 cut(s) 417
BstBI TTCGAA 2 cut(s) 272, 475
BstC8I GCNNGC 2 cut(s) 418, 664
BstDEI CTNAG 5 cut(s) 92, 280, 362, 549, 639
BstF5I GGATG 2 cut(s) 40, 587
BstKTI GATC 2 cut(s) 235, 373
BstMAI GTCTC 1 cut(s) 283
BstMBI GATC 2 cut(s) 232, 370
BstMWI GCNNNNNNNGC 3 cut(s) 79, 417, 602
BstSFI CTRYAG 1 cut(s) 660
BstV1I GCAGC 3 cut(s) 60, 301, 488
BstX2I RGATCY 1 cut(s) 232
BstYI RGATCY 1 cut(s) 232
BsuRI GGCC 2 cut(s) 339, 666
BtsCI GGATG 2 cut(s) 40, 587
Cac8I GCNNGC 2 cut(s) 418, 664
CaiI CAGNNNCTG 1 cut(s) 287
CseI GACGC 1 cut(s) 179
Csp6I GTAC 2 cut(s) 105, 144
CviAII CATG 2 cut(s) 20, 691
CviJI RGCY 9 cut(s) 6, 14, 73, 127, 266, 314, 339, 596, 666
CviKI_1 RGCY 9 cut(s) 6, 14, 73, 127, 266, 314, 339, 596, 666
CviQI GTAC 2 cut(s) 105, 144
DdeI CTNAG 5 cut(s) 92, 280, 362, 549, 639
DpnI GATC 2 cut(s) 234, 372
DpnII GATC 2 cut(s) 232, 370
Eco147I AGGCCT 1 cut(s) 666
Eco57I CTGAAG 3 cut(s) 273, 432, 555
Esp3I CGTCTC 1 cut(s) 283
FaeI CATG 2 cut(s) 23, 694
FaiI YATR 7 cut(s) 21, 202, 311, 593, 603, 608, 692
FalI AAGNNNNNCTT 2 cut(s) 430, 462
FatI CATG 2 cut(s) 19, 690
Fnu4HI GCNGC 3 cut(s) 74, 315, 502
FokI GGATG 2 cut(s) 27, 574
Fsp4HI GCNGC 3 cut(s) 74, 315, 502
FspBI CTAG 4 cut(s) 11, 98, 398, 597
GluI GCNGC 3 cut(s) 74, 315, 502
HaeIII GGCC 2 cut(s) 339, 666
HgaI GACGC 1 cut(s) 179
Hin1II CATG 2 cut(s) 23, 694
HincII GTYRAC 2 cut(s) 355, 645
HindII GTYRAC 2 cut(s) 355, 645
HindIII AAGCTT 1 cut(s) 264
HinfI GANTC 5 cut(s) 90, 452, 472, 527, 575
HphI GGTGA 1 cut(s) 423
Hpy166II GTNNAC 3 cut(s) 185, 355, 645
Hpy188I TCNGA 4 cut(s) 244, 451, 550, 574
Hpy188III TCNNGA 5 cut(s) 150, 164, 217, 464, 524
Hpy8I GTNNAC 3 cut(s) 185, 355, 645
Hpy99I CGWCG 1 cut(s) 173
HpyAV CCTTC 2 cut(s) 350, 694
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 8 cut(s) 76, 82, 195, 259, 293, 317, 504, 662
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 417, 602
HpyF3I CTNAG 5 cut(s) 92, 280, 362, 549, 639
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 2 cut(s) 23, 694
Kzo9I GATC 2 cut(s) 232, 370
LmnI GCTCC 1 cut(s) 491
LpnPI CCDG 9 cut(s) 73, 149, 181, 267, 444, 454, 516, 648, 689
Lsp1109I GCAGC 3 cut(s) 60, 301, 488
LweI GCATC 2 cut(s) 268, 556
MaeI CTAG 4 cut(s) 11, 98, 398, 597
MaeII ACGT 1 cut(s) 276
MalI GATC 2 cut(s) 234, 372
MboI GATC 2 cut(s) 232, 370
MboII GAAGA 9 cut(s) 35, 261, 294, 438, 504, 575, 576, 615, 625
MfeI CAATTG 1 cut(s) 710
MflI RGATCY 1 cut(s) 232
MhlI GDGCHC 1 cut(s) 555
MluCI AATT 4 cut(s) 55, 306, 480, 710
MlyI GAGTC 2 cut(s) 84, 446
MnlI CCTC 3 cut(s) 157, 368, 550
MseI TTAA 1 cut(s) 422
MunI CAATTG 1 cut(s) 710
Mva1269I GAATGC 2 cut(s) 31, 611
MwoI GCNNNNNNNGC 3 cut(s) 79, 417, 602
NdeII GATC 2 cut(s) 232, 370
NlaIII CATG 2 cut(s) 23, 694
NspV TTCGAA 2 cut(s) 272, 475
PceI AGGCCT 1 cut(s) 666
PctI GAATGC 2 cut(s) 31, 611
PfeI GAWTC 3 cut(s) 472, 527, 575
PflFI GACNNNGTC 1 cut(s) 93
PkrI GCNGC 3 cut(s) 75, 316, 503
PleI GAGTC 2 cut(s) 84, 446
PpsI GAGTC 2 cut(s) 84, 446
PstI CTGCAG 1 cut(s) 664
PstNI CAGNNNCTG 1 cut(s) 287
PsuI RGATCY 1 cut(s) 232
PsyI GACNNNGTC 1 cut(s) 93
RsaI GTAC 2 cut(s) 106, 145
RsaNI GTAC 2 cut(s) 105, 144
SaqAI TTAA 1 cut(s) 422
SatI GCNGC 3 cut(s) 74, 315, 502
Sau3AI GATC 2 cut(s) 232, 370
SchI GAGTC 2 cut(s) 84, 446
SduI GDGCHC 1 cut(s) 555
SfaNI GCATC 2 cut(s) 268, 556
SfcI CTRYAG 1 cut(s) 660
SfuI TTCGAA 2 cut(s) 272, 475
Sse9I AATT 4 cut(s) 55, 306, 480, 710
SseBI AGGCCT 1 cut(s) 666
SspMI CTAG 4 cut(s) 11, 98, 398, 597
StuI AGGCCT 1 cut(s) 666
TaaI ACNGT 1 cut(s) 109
TaiI ACGT 1 cut(s) 279
TaqI TCGA 3 cut(s) 151, 272, 475
TasI AATT 4 cut(s) 55, 306, 480, 710
TfiI GAWTC 3 cut(s) 472, 527, 575
Tru1I TTAA 1 cut(s) 422
Tru9I TTAA 1 cut(s) 422
TseI GCWGC 3 cut(s) 73, 314, 501
TspDTI ATGAA 2 cut(s) 36, 576
TspGWI ACGGA 1 cut(s) 92
Tth111I GACNNNGTC 1 cut(s) 93
XapI RAATTY 1 cut(s) 55
XspI CTAG 4 cut(s) 11, 98, 398, 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.