pycom02g24870

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0028592
Physical Location & Seq
Forward (+)
22965843 .. 22966553
711 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 711 bp
ATGGTTTTTCTTCCGCCGCGTCCCAACTTTGGGGCTCTTTCTCGCTGTGTGTGTTTTTGTAACCATAAACTCCTTGAGGCAAAAAAAGCACCATGGCTTTCCCGTTTGACCAGTGTTTGGCATGTTCCCCCCTCTCTCTCTGGCACTACTCCAACCCAACCCAACCCATATGAGTTGCTCTCCAAAGTTCTTTGCCGCCAAAATGGAACGATTAGATTCAAAGGCCCTTGGTTTAGCAGCCTCGTATGCGTCATCACTTGCGACCCTCGTAACTTGGAGTCCCTTCTCAAGACCAAGTTCTCCAATTTCCTCAAAGGTCCTTATTTCCAAGACACCGTTCGGGATCTTCTCCGTGATGGCATATTCAGTGCAGACCGCGATAAGTGGCAGCGCCAAAGGAAGATGGCGAGCTTTGAGTTCCAGTCAGCCAAGTTCCGGAAACTGACCGTGGATTCATTGTTTGAGCTGGTTCACGGTCGGTTGTTACCTGTTTTAGAGGACTCCATCAAACACTCGGCCCCAATCAACCTCCAAGACGTTCTTATGAGGTTGACTTTTGACAACGTTTGCATGATCGCATTTAGGGTCGATCCCGATTGCTTGCAGCCAGGTTTACCCAAAATACCATTTGCGCAGGCTATTGAGGAGGTGACAGAAGGAACGTTGATGCGCTTTGTTGTCCCCCTGTGCGTGTGGAAGGCCACGAGGTAA

Protein Analysis

237

Amino Acids

27.03

Weight (kDa)

9.41

Isoelectric Point (pI)

34.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 78 - 203 2.2e-09 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0028592)

Species Orthologous Gene IDs
malus_domestica MD02G1295900.v1.1
pyrus_communis pycom02g24870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 633
AccB7I CCANNNNNTGG 1 cut(s) 117
AccII CGCG 2 cut(s) 19, 378
AccIII TCCGGA 1 cut(s) 435
AciI CCGC 4 cut(s) 14, 17, 196, 376
AclI AACGTT 2 cut(s) 564, 662
AclWI GGATC 2 cut(s) 351, 584
AfiI CCNNNNNNNGG 4 cut(s) 29, 30, 117, 435
AgsI TTSAA 1 cut(s) 220
AjnI CCWGG 1 cut(s) 607
AjuI GAANNNNNNNTTGG 2 cut(s) 321, 353
AluBI AGCT 2 cut(s) 411, 466
AluI AGCT 2 cut(s) 411, 466
AlwI GGATC 2 cut(s) 351, 584
Aor13HI TCCGGA 1 cut(s) 435
AoxI GGCC 3 cut(s) 223, 516, 699
ApeKI GCWGC 3 cut(s) 237, 388, 604
AspLEI GCGC 3 cut(s) 393, 634, 672
AspS9I GGNCC 3 cut(s) 224, 317, 517
AsuHPI GGTGA 1 cut(s) 661
AvaII GGWCC 1 cut(s) 317
BanII GRGCYC 1 cut(s) 37
BauI CACGAG 1 cut(s) 703
BbvI GCAGC 3 cut(s) 249, 400, 616
BccI CCATC 3 cut(s) 350, 397, 512
BciT130I CCWGG 1 cut(s) 609
BfoI RGCGCY 1 cut(s) 394
BisI GCNGC 5 cut(s) 17, 196, 238, 389, 605
BlsI GCNGC 5 cut(s) 18, 197, 239, 390, 606
Bme1390I CCNGG 1 cut(s) 609
Bme18I GGWCC 1 cut(s) 317
BmgT120I GGNCC 3 cut(s) 224, 317, 517
BmiI GGNNCC 1 cut(s) 519
BmrFI CCNGG 1 cut(s) 609
BmsI GCATC 1 cut(s) 657
BplI GAGNNNNNCTC 2 cut(s) 164, 196
BpuEI CTTGAG 2 cut(s) 95, 272
BsaJI CCNNGG 3 cut(s) 92, 227, 447
BsaWI WCCGGW 1 cut(s) 435
Bsc4I CCNNNNNNNGG 4 cut(s) 29, 30, 117, 435
Bse1I ACTGG 2 cut(s) 111, 421
BseAI TCCGGA 1 cut(s) 435
BseBI CCWGG 1 cut(s) 609
BseDI CCNNGG 3 cut(s) 92, 227, 447
BseLI CCNNNNNNNGG 4 cut(s) 29, 30, 117, 435
BseNI ACTGG 2 cut(s) 111, 421
BseRI GAGGAG 1 cut(s) 659
BseXI GCAGC 3 cut(s) 249, 400, 616
BsgI GTGCAG 1 cut(s) 390
Bsh1236I CGCG 2 cut(s) 19, 378
Bsh1285I CGRYCG 1 cut(s) 478
BshFI GGCC 3 cut(s) 225, 518, 701
BsiEI CGRYCG 1 cut(s) 478
BsiSI CCGG 1 cut(s) 436
BslFI GGGAC 3 cut(s) 6, 265, 665
BslI CCNNNNNNNGG 4 cut(s) 29, 30, 117, 435
BsmFI GGGAC 3 cut(s) 6, 265, 665
BsnI GGCC 3 cut(s) 225, 518, 701
Bsp1286I GDGCHC 1 cut(s) 37
Bsp13I TCCGGA 1 cut(s) 435
Bsp143I GATC 3 cut(s) 343, 573, 589
Bsp19I CCATGG 1 cut(s) 92
BspACI CCGC 4 cut(s) 14, 17, 196, 376
BspANI GGCC 3 cut(s) 225, 518, 701
BspEI TCCGGA 1 cut(s) 435
BspFNI CGCG 2 cut(s) 19, 378
BspLI GGNNCC 1 cut(s) 519
BspPI GGATC 2 cut(s) 351, 584
BsrI ACTGG 2 cut(s) 111, 421
BssECI CCNNGG 3 cut(s) 92, 227, 447
BssMI GATC 3 cut(s) 343, 573, 589
BssSI CACGAG 1 cut(s) 703
BssT1I CCWWGG 2 cut(s) 92, 227
Bst2BI CACGAG 1 cut(s) 703
Bst2UI CCWGG 1 cut(s) 609
Bst4CI ACNGT 3 cut(s) 337, 448, 476
BstC8I GCNNGC 3 cut(s) 409, 602, 636
BstDSI CCRYGG 2 cut(s) 92, 447
BstFNI CGCG 2 cut(s) 19, 378
BstH2I RGCGCY 1 cut(s) 394
BstHHI GCGC 3 cut(s) 393, 634, 672
BstKTI GATC 3 cut(s) 346, 576, 592
BstMBI GATC 3 cut(s) 343, 573, 589
BstMCI CGRYCG 1 cut(s) 478
BstMWI GCNNNNNNNGC 2 cut(s) 86, 246
BstNI CCWGG 1 cut(s) 609
BstNSI RCATGY 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 607
BstUI CGCG 2 cut(s) 19, 378
BstV1I GCAGC 3 cut(s) 249, 400, 616
BstX2I RGATCY 1 cut(s) 343
BstYI RGATCY 1 cut(s) 343
BsuRI GGCC 3 cut(s) 225, 518, 701
BtgI CCRYGG 2 cut(s) 92, 447
BtsIMutI CAGTG 2 cut(s) 118, 373
Cac8I GCNNGC 3 cut(s) 409, 602, 636
CfoI GCGC 3 cut(s) 393, 634, 672
Cfr13I GGNCC 3 cut(s) 224, 317, 517
CseI GACGC 2 cut(s) 8, 238
CviAII CATG 3 cut(s) 93, 122, 571
DpnI GATC 3 cut(s) 345, 575, 591
DpnII GATC 3 cut(s) 343, 573, 589
Eco130I CCWWGG 2 cut(s) 92, 227
Eco24I GRGCYC 1 cut(s) 37
Eco47I GGWCC 1 cut(s) 317
EcoO109I RGGNCCY 2 cut(s) 224, 317
EcoRII CCWGG 1 cut(s) 607
EcoT14I CCWWGG 2 cut(s) 92, 227
EcoT38I GRGCYC 1 cut(s) 37
ErhI CCWWGG 2 cut(s) 92, 227
FaeI CATG 3 cut(s) 96, 125, 574
FaiI YATR 9 cut(s) 66, 94, 123, 169, 171, 247, 362, 545, 572
FalI AAGNNNNNCTT 2 cut(s) 525, 557
FaqI GGGAC 3 cut(s) 6, 265, 665
FatI CATG 3 cut(s) 92, 121, 570
FauNDI CATATG 1 cut(s) 169
Fnu4HI GCNGC 5 cut(s) 17, 196, 238, 389, 605
FriOI GRGCYC 1 cut(s) 37
Fsp4HI GCNGC 5 cut(s) 17, 196, 238, 389, 605
FspI TGCGCA 1 cut(s) 633
GlaI GCGC 3 cut(s) 392, 633, 671
GluI GCNGC 5 cut(s) 17, 196, 238, 389, 605
HaeII RGCGCY 1 cut(s) 394
HaeIII GGCC 3 cut(s) 225, 518, 701
HapII CCGG 1 cut(s) 436
HgaI GACGC 2 cut(s) 8, 238
HhaI GCGC 3 cut(s) 393, 634, 672
Hin1II CATG 3 cut(s) 96, 125, 574
Hin6I GCGC 3 cut(s) 391, 632, 670
HinP1I GCGC 3 cut(s) 391, 632, 670
HincII GTYRAC 1 cut(s) 552
HindII GTYRAC 1 cut(s) 552
HinfI GANTC 4 cut(s) 216, 278, 452, 500
HpaII CCGG 1 cut(s) 436
HphI GGTGA 1 cut(s) 661
Hpy166II GTNNAC 3 cut(s) 472, 552, 614
Hpy188III TCNNGA 4 cut(s) 289, 341, 436, 593
Hpy8I GTNNAC 3 cut(s) 472, 552, 614
HpyAV CCTTC 3 cut(s) 293, 650, 691
HpyCH4III ACNGT 3 cut(s) 337, 448, 476
HpyCH4IV ACGT 3 cut(s) 537, 564, 662
HpyCH4V TGCA 3 cut(s) 371, 570, 604
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 246
HpySE526I ACGT 3 cut(s) 537, 564, 662
Hsp92II CATG 3 cut(s) 96, 125, 574
HspAI GCGC 3 cut(s) 391, 632, 670
Kpn2I TCCGGA 1 cut(s) 435
Kzo9I GATC 3 cut(s) 343, 573, 589
Lsp1109I GCAGC 3 cut(s) 249, 400, 616
LweI GCATC 1 cut(s) 657
MaeII ACGT 3 cut(s) 537, 564, 662
MaeIII GTNAC 4 cut(s) 59, 269, 483, 649
MalI GATC 3 cut(s) 345, 575, 591
MboI GATC 3 cut(s) 343, 573, 589
MboII GAAGA 2 cut(s) 338, 412
MflI RGATCY 1 cut(s) 343
MhlI GDGCHC 1 cut(s) 37
MluCI AATT 1 cut(s) 304
MlyI GAGTC 2 cut(s) 287, 494
MmeI TCCRAC 1 cut(s) 176
MroI TCCGGA 1 cut(s) 435
MspI CCGG 1 cut(s) 436
MspR9I CCNGG 1 cut(s) 609
MvaI CCWGG 1 cut(s) 609
MvnI CGCG 2 cut(s) 19, 378
MwoI GCNNNNNNNGC 2 cut(s) 86, 246
NcoI CCATGG 1 cut(s) 92
NdeI CATATG 1 cut(s) 169
NdeII GATC 3 cut(s) 343, 573, 589
NlaIII CATG 3 cut(s) 96, 125, 574
NlaIV GGNNCC 1 cut(s) 519
NmeAIII GCCGAG 1 cut(s) 494
NmuCI GTSAC 1 cut(s) 649
NsbI TGCGCA 1 cut(s) 633
NspI RCATGY 1 cut(s) 125
PfeI GAWTC 2 cut(s) 216, 452
PflMI CCANNNNNTGG 1 cut(s) 117
PkrI GCNGC 5 cut(s) 18, 197, 239, 390, 606
PleI GAGTC 2 cut(s) 286, 494
PpsI GAGTC 2 cut(s) 286, 494
PpuMI RGGWCCY 1 cut(s) 317
Psp1406I AACGTT 2 cut(s) 564, 662
Psp5II RGGWCCY 1 cut(s) 317
Psp6I CCWGG 1 cut(s) 607
PspGI CCWGG 1 cut(s) 607
PspN4I GGNNCC 1 cut(s) 519
PspPI GGNCC 3 cut(s) 224, 317, 517
PspPPI RGGWCCY 1 cut(s) 317
PsuI RGATCY 1 cut(s) 343
SatI GCNGC 5 cut(s) 17, 196, 238, 389, 605
Sau3AI GATC 3 cut(s) 343, 573, 589
Sau96I GGNCC 3 cut(s) 224, 317, 517
SchI GAGTC 2 cut(s) 287, 494
ScrFI CCNGG 1 cut(s) 609
SduI GDGCHC 1 cut(s) 37
SfaNI GCATC 1 cut(s) 657
SinI GGWCC 1 cut(s) 317
SmlI CTYRAG 2 cut(s) 74, 287
SmoI CTYRAG 2 cut(s) 74, 287
Sse9I AATT 1 cut(s) 304
SsiI CCGC 4 cut(s) 14, 17, 196, 376
StyD4I CCNGG 1 cut(s) 607
StyI CCWWGG 2 cut(s) 92, 227
TaaI ACNGT 3 cut(s) 337, 448, 476
TaiI ACGT 3 cut(s) 540, 567, 665
TaqI TCGA 1 cut(s) 588
TasI AATT 1 cut(s) 304
TauI GCSGC 2 cut(s) 19, 198
TfiI GAWTC 2 cut(s) 216, 452
TscAI CASTG 2 cut(s) 118, 373
TseFI GTSAC 1 cut(s) 649
TseI GCWGC 3 cut(s) 237, 388, 604
Tsp45I GTSAC 1 cut(s) 649
TspDTI ATGAA 1 cut(s) 444
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 2 cut(s) 118, 373
Van91I CCANNNNNTGG 1 cut(s) 117
VpaK11BI GGWCC 1 cut(s) 317
XceI RCATGY 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.