pycom03g00090

TATA-binding protein-associated factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0028498
Physical Location & Seq
Forward (+)
67829 .. 74455
6627 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1212 bp
ATGGCCCAGCAGTCCTCTCGTCTCCATCGCCTCCTCACTCTCTTGGACACTGGTTCAACCCAAGCGACCAGATTTACAGCTGCTCGGCAGATAGGGGACATTGCCAAAGCGCATCCTCAGGACTTGAGCACTCTGTTGAAAAAGGTTTCCCAATATCTTCGTAGCAAAAACTGGGATACAAGGGTTGCTGCCGCTCATGCTGTTGGGGCTATTGCTGAGAATGTGAAGCACACCTCTTTGATTGAACTCTTTACTTGTATTAAATCGAAGATGGCTGATGCTGGGATATCTGGTTCTATTGAAGATATGGTTGCCTTCCCTATTTTTGATTCTAACGTTGCTGGCACGTCATTTAGAAGCTTTGATCTCAAAAAGGTTTTGGAGTTTGGTGCTCTGTTTGCATCTGGGGGACAGGAGTATGATGTTGCAAATGATCATATGAAGAGCCCAAGCGAGAAACTGGCTCGTCAGAAACAAACACTTCGACGTCGTTTAGGTTTGGATATATGTGAGCAGTTCATGGATGTGAATGATATGATAAAAGATGAAGACTTTATTTTACACAACTCGCATGGGAACGGGACTAATCCAAGAGTTTATACATCACATAGTATTCAGCAATTAGTTGCAAATATGGTTCCTAGTGTCTTATCAAAAAGGCCAAGTCCCAGGGAATTGAATCTTTTAAAACGAAAAGCAAAAATCACTTCGAAAGACCAGTCAAAAGCTTGGTCTGAAAATGGGGATATGGAGGTGTCATGTGCTCAAAATATACCACCAAAGGGGGCATGTCCTGACTCATTCGGGACTAATAAGGAATTTTTGGACCTGGATAATCACGAAGAAAAAGTTGAACATGATGGAGAAGGGCGGTGGCCTTTTCATAGCTTCGTTGAGGAACTAATCTTAGATATGTTTGATCCAGTTTGGGAGGTACGCCATGGTAGCGTGATGGCTTTGAGAGAAATTTTAACCCATCAAGGTGTTTCTGCTGGAGTATTTATGCCTGACTTGAGCCTGGAGGGTGCAATGTTTGTGGAATTAGAAAATAAATGGACGTCATCTACAATGAAGAGAGACAGAGAGATTGATTTGAATATGCAAGTTCCTATTGATGAATCTGAACCAGAGCTGAAAAAACCAAAGTTTGAAGATGTTACATCTCCATTTATGGATACTATGGGTCTCTGCCAGCAAGAGTGGGGATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0048509 GO:0048518 GO:0050789 GO:0050793 GO:0051094 GO:0065007 GO:1902183 GO:1902185
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3535 HEAT HEAT_EZ Helicase_C SNF2_N

Protein Analysis

404

Amino Acids

45.28

Weight (kDa)

5.67

Isoelectric Point (pI)

46.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0028498)

Species Orthologous Gene IDs
malus_domestica MD03G1000600.v1.1
pyrus_communis pycom03g00090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 490, 1061
AccBSI CCGCTC 1 cut(s) 194
AciI CCGC 2 cut(s) 192, 871
AclI AACGTT 1 cut(s) 336
AclWI GGATC 1 cut(s) 914
AcsI RAATTY 2 cut(s) 818, 966
AcyI GRCGYC 2 cut(s) 487, 1058
AfaI GTAC 1 cut(s) 936
AfiI CCNNNNNNNGG 1 cut(s) 782
AgsI TTSAA 8 cut(s) 57, 139, 245, 302, 679, 854, 1096, 1151
AjiI CACGTC 1 cut(s) 348
AjnI CCWGG 3 cut(s) 668, 828, 1017
AloI GAACNNNNNNTCC 2 cut(s) 277, 309
AluBI AGCT 5 cut(s) 80, 360, 728, 888, 1132
AluI AGCT 5 cut(s) 80, 360, 728, 888, 1132
Alw21I GWGCWC 3 cut(s) 131, 394, 766
Alw26I GTCTC 3 cut(s) 26, 1071, 1190
AlwI GGATC 1 cut(s) 914
AoxI GGCC 3 cut(s) 3, 659, 875
ApeKI GCWGC 2 cut(s) 80, 188
ApoI RAATTY 2 cut(s) 818, 966
ArsI GACNNNNNNTTYG 4 cut(s) 704, 716, 736, 748
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 2 cut(s) 4, 826
AsuII TTCGAA 1 cut(s) 710
AvaII GGWCC 1 cut(s) 826
AxyI CCTNAGG 1 cut(s) 117
BaeI ACNNNNGTAYC 2 cut(s) 168, 201
BanII GRGCYC 1 cut(s) 449
BbsI GAAGAC 1 cut(s) 555
Bbv12I GWGCWC 3 cut(s) 131, 394, 766
BbvI GCAGC 2 cut(s) 67, 175
BccI CCATC 5 cut(s) 33, 265, 854, 946, 984
BcgI CGANNNNNNTGC 1 cut(s) 33
BciT130I CCWGG 3 cut(s) 670, 830, 1019
BciVI GTATCC 2 cut(s) 169, 1168
BclI TGATCA 1 cut(s) 433
BcoDI GTCTC 3 cut(s) 26, 1071, 1190
BfaI CTAG 1 cut(s) 642
BfuI GTATCC 2 cut(s) 169, 1168
BisI GCNGC 3 cut(s) 81, 189, 192
BlsI GCNGC 3 cut(s) 82, 190, 193
Bme1390I CCNGG 3 cut(s) 670, 830, 1019
Bme18I GGWCC 1 cut(s) 826
BmgBI CACGTC 1 cut(s) 348
BmgT120I GGNCC 2 cut(s) 4, 826
BmiI GGNNCC 1 cut(s) 639
BmrFI CCNGG 3 cut(s) 670, 830, 1019
BmrI ACTGGG 1 cut(s) 181
BmsI GCATC 3 cut(s) 121, 268, 410
BmuI ACTGGG 1 cut(s) 181
BpiI GAAGAC 1 cut(s) 555
BpmI CTGGAG 2 cut(s) 1014, 1040
Bpu14I TTCGAA 1 cut(s) 710
BpuEI CTTGAG 2 cut(s) 145, 1033
BsaHI GRCGYC 2 cut(s) 487, 1058
BsaI GGTCTC 1 cut(s) 1190
BsaJI CCNNGG 3 cut(s) 668, 669, 940
Bsc4I CCNNNNNNNGG 1 cut(s) 782
Bse1I ACTGG 5 cut(s) 55, 176, 465, 718, 923
Bse21I CCTNAGG 1 cut(s) 117
Bse3DI GCAATG 2 cut(s) 99, 1035
BseBI CCWGG 3 cut(s) 670, 830, 1019
BseDI CCNNGG 3 cut(s) 668, 669, 940
BseGI GGATG 2 cut(s) 112, 529
BseLI CCNNNNNNNGG 1 cut(s) 782
BseMI GCAATG 2 cut(s) 99, 1035
BseMII CTCAG 2 cut(s) 131, 207
BseNI ACTGG 5 cut(s) 55, 176, 465, 718, 923
BseRI GAGGAG 1 cut(s) 23
BseXI GCAGC 2 cut(s) 67, 175
BseYI CCCAGC 2 cut(s) 6, 281
BshFI GGCC 3 cut(s) 5, 661, 877
BsiHKAI GWGCWC 3 cut(s) 131, 394, 766
BslFI GGGAC 5 cut(s) 110, 423, 595, 651, 820
BslI CCNNNNNNNGG 1 cut(s) 782
BsmAI GTCTC 3 cut(s) 26, 1071, 1190
BsmBI CGTCTC 1 cut(s) 26
BsmFI GGGAC 5 cut(s) 110, 423, 595, 651, 820
BsnI GGCC 3 cut(s) 5, 661, 877
Bso31I GGTCTC 1 cut(s) 1190
Bsp119I TTCGAA 1 cut(s) 710
Bsp1286I GDGCHC 4 cut(s) 131, 394, 449, 766
Bsp143I GATC 3 cut(s) 364, 433, 919
Bsp19I CCATGG 1 cut(s) 940
BspACI CCGC 2 cut(s) 192, 871
BspANI GGCC 3 cut(s) 5, 661, 877
BspCNI CTCAG 2 cut(s) 130, 208
BspLI GGNNCC 1 cut(s) 639
BspPI GGATC 1 cut(s) 914
BspQI GCTCTTC 1 cut(s) 437
BspT104I TTCGAA 1 cut(s) 710
BspTNI GGTCTC 1 cut(s) 1190
BsrBI CCGCTC 1 cut(s) 194
BsrDI GCAATG 2 cut(s) 99, 1035
BsrI ACTGG 5 cut(s) 55, 176, 465, 718, 923
BssECI CCNNGG 3 cut(s) 668, 669, 940
BssMI GATC 3 cut(s) 364, 433, 919
BssNI GRCGYC 2 cut(s) 487, 1058
BssT1I CCWWGG 1 cut(s) 940
Bst2UI CCWGG 3 cut(s) 670, 830, 1019
Bst6I CTCTTC 2 cut(s) 437, 1067
BstACI GRCGYC 2 cut(s) 487, 1058
BstBI TTCGAA 1 cut(s) 710
BstC8I GCNNGC 2 cut(s) 343, 1193
BstDEI CTNAG 3 cut(s) 117, 216, 907
BstDSI CCRYGG 1 cut(s) 940
BstF5I GGATG 2 cut(s) 112, 529
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 3 cut(s) 367, 436, 922
BstMAI GTCTC 3 cut(s) 26, 1071, 1190
BstMBI GATC 3 cut(s) 364, 433, 919
BstMWI GCNNNNNNNGC 4 cut(s) 197, 206, 398, 945
BstNI CCWGG 3 cut(s) 670, 830, 1019
BstNSI RCATGY 1 cut(s) 792
BstSCI CCNGG 3 cut(s) 668, 828, 1017
BstV1I GCAGC 2 cut(s) 67, 175
BstV2I GAAGAC 1 cut(s) 555
Bsu36I CCTNAGG 1 cut(s) 117
BsuI GTATCC 2 cut(s) 169, 1168
BsuRI GGCC 3 cut(s) 5, 661, 877
BtgI CCRYGG 1 cut(s) 940
BtgZI GCGATG 1 cut(s) 11
BtrI CACGTC 1 cut(s) 348
BtsCI GGATG 2 cut(s) 112, 529
BtsIMutI CAGTG 1 cut(s) 48
Cac8I GCNNGC 2 cut(s) 343, 1193
CfoI GCGC 1 cut(s) 112
Cfr13I GGNCC 2 cut(s) 4, 826
Csp6I GTAC 1 cut(s) 935
CspCI CAANNNNNGTGG 2 cut(s) 1017, 1052
CviAII CATG 7 cut(s) 197, 520, 572, 759, 789, 857, 941
CviQI GTAC 1 cut(s) 935
DdeI CTNAG 3 cut(s) 117, 216, 907
DpnI GATC 3 cut(s) 366, 435, 921
DpnII GATC 3 cut(s) 364, 433, 919
DraI TTTAAA 1 cut(s) 687
Eam1104I CTCTTC 2 cut(s) 437, 1067
EarI CTCTTC 2 cut(s) 437, 1067
Eco130I CCWWGG 1 cut(s) 940
Eco24I GRGCYC 1 cut(s) 449
Eco31I GGTCTC 1 cut(s) 1190
Eco32I GATATC 1 cut(s) 288
Eco47I GGWCC 1 cut(s) 826
Eco81I CCTNAGG 1 cut(s) 117
EcoRII CCWGG 3 cut(s) 668, 828, 1017
EcoRV GATATC 1 cut(s) 288
EcoT14I CCWWGG 1 cut(s) 940
EcoT38I GRGCYC 1 cut(s) 449
ErhI CCWWGG 1 cut(s) 940
Esp3I CGTCTC 1 cut(s) 26
FaeI CATG 7 cut(s) 200, 523, 575, 762, 792, 860, 944
FaqI GGGAC 5 cut(s) 110, 423, 595, 651, 820
FatI CATG 7 cut(s) 196, 519, 571, 758, 788, 856, 940
FauNDI CATATG 1 cut(s) 438
FbaI TGATCA 1 cut(s) 433
Fnu4HI GCNGC 3 cut(s) 81, 189, 192
FokI GGATG 2 cut(s) 99, 536
FriOI GRGCYC 1 cut(s) 449
Fsp4HI GCNGC 3 cut(s) 81, 189, 192
FspBI CTAG 1 cut(s) 642
GlaI GCGC 1 cut(s) 111
GluI GCNGC 3 cut(s) 81, 189, 192
GsaI CCCAGC 2 cut(s) 10, 285
GsuI CTGGAG 2 cut(s) 1014, 1040
HaeIII GGCC 3 cut(s) 5, 661, 877
HhaI GCGC 1 cut(s) 112
Hin1I GRCGYC 2 cut(s) 487, 1058
Hin1II CATG 7 cut(s) 200, 523, 575, 762, 792, 860, 944
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HindIII AAGCTT 2 cut(s) 358, 726
HinfI GANTC 4 cut(s) 329, 679, 797, 1118
Hpy188I TCNGA 3 cut(s) 471, 736, 1123
Hpy188III TCNNGA 4 cut(s) 119, 794, 805, 839
Hpy99I CGWCG 2 cut(s) 489, 492
HpyAV CCTTC 2 cut(s) 325, 860
HpyCH4IV ACGT 4 cut(s) 336, 347, 487, 1058
HpyCH4V TGCA 5 cut(s) 401, 428, 629, 1028, 1102
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 206, 398, 945
HpyF3I CTNAG 3 cut(s) 117, 216, 907
HpySE526I ACGT 4 cut(s) 336, 347, 487, 1058
Hsp92I GRCGYC 2 cut(s) 487, 1058
Hsp92II CATG 7 cut(s) 200, 523, 575, 762, 792, 860, 944
HspAI GCGC 1 cut(s) 110
Ksp22I TGATCA 1 cut(s) 433
Kzo9I GATC 3 cut(s) 364, 433, 919
LguI GCTCTTC 1 cut(s) 437
Lsp1109I GCAGC 2 cut(s) 67, 175
LweI GCATC 3 cut(s) 121, 268, 410
MaeI CTAG 1 cut(s) 642
MaeII ACGT 4 cut(s) 336, 347, 487, 1058
MaeIII GTNAC 1 cut(s) 1156
MalI GATC 3 cut(s) 366, 435, 921
MbiI CCGCTC 1 cut(s) 194
MboI GATC 3 cut(s) 364, 433, 919
MboII GAAGA 8 cut(s) 149, 280, 314, 454, 560, 854, 1084, 1163
MhlI GDGCHC 4 cut(s) 131, 394, 449, 766
MluCI AATT 5 cut(s) 620, 674, 818, 966, 1040
MlyI GAGTC 1 cut(s) 791
MnlI CCTC 9 cut(s) 25, 41, 44, 126, 244, 745, 889, 925, 1015
MseI TTAA 3 cut(s) 261, 686, 971
MslI CAYNNNNRTG 2 cut(s) 524, 981
MspA1I CMGCKG 1 cut(s) 80
MspR9I CCNGG 3 cut(s) 670, 830, 1019
MvaI CCWGG 3 cut(s) 670, 830, 1019
MwoI GCNNNNNNNGC 4 cut(s) 197, 206, 398, 945
NcoI CCATGG 1 cut(s) 940
NdeI CATATG 1 cut(s) 438
NdeII GATC 3 cut(s) 364, 433, 919
NlaIII CATG 7 cut(s) 200, 523, 575, 762, 792, 860, 944
NlaIV GGNNCC 1 cut(s) 639
NmeAIII GCCGAG 1 cut(s) 64
NspI RCATGY 1 cut(s) 792
NspV TTCGAA 1 cut(s) 710
PasI CCCWGGG 1 cut(s) 669
PciSI GCTCTTC 1 cut(s) 437
PfeI GAWTC 3 cut(s) 329, 679, 1118
PkrI GCNGC 3 cut(s) 82, 190, 193
PleI GAGTC 1 cut(s) 791
PpsI GAGTC 1 cut(s) 791
Psp1406I AACGTT 1 cut(s) 336
Psp6I CCWGG 3 cut(s) 668, 828, 1017
PspFI CCCAGC 2 cut(s) 6, 281
PspGI CCWGG 3 cut(s) 668, 828, 1017
PspN4I GGNNCC 1 cut(s) 639
PspPI GGNCC 2 cut(s) 4, 826
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 1 cut(s) 936
RsaNI GTAC 1 cut(s) 935
RseI CAYNNNNRTG 2 cut(s) 524, 981
SapI GCTCTTC 1 cut(s) 437
SaqAI TTAA 3 cut(s) 261, 686, 971
SatI GCNGC 3 cut(s) 81, 189, 192
Sau3AI GATC 3 cut(s) 364, 433, 919
Sau96I GGNCC 2 cut(s) 4, 826
SchI GAGTC 1 cut(s) 791
ScrFI CCNGG 3 cut(s) 670, 830, 1019
SduI GDGCHC 4 cut(s) 131, 394, 449, 766
SfaNI GCATC 3 cut(s) 121, 268, 410
SfuI TTCGAA 1 cut(s) 710
SinI GGWCC 1 cut(s) 826
SmiMI CAYNNNNRTG 2 cut(s) 524, 981
SmlI CTYRAG 2 cut(s) 124, 1012
SmoI CTYRAG 2 cut(s) 124, 1012
Sse9I AATT 5 cut(s) 620, 674, 818, 966, 1040
SsiI CCGC 2 cut(s) 192, 871
SspMI CTAG 1 cut(s) 642
StyD4I CCNGG 3 cut(s) 668, 828, 1017
StyI CCWWGG 1 cut(s) 940
TaiI ACGT 4 cut(s) 339, 350, 490, 1061
TaqI TCGA 3 cut(s) 266, 484, 710
TasI AATT 5 cut(s) 620, 674, 818, 966, 1040
TauI GCSGC 1 cut(s) 194
TfiI GAWTC 3 cut(s) 329, 679, 1118
Tru1I TTAA 3 cut(s) 261, 686, 971
Tru9I TTAA 3 cut(s) 261, 686, 971
TscAI CASTG 1 cut(s) 55
TseI GCWGC 2 cut(s) 80, 188
TspDTI ATGAA 6 cut(s) 455, 508, 561, 872, 1085, 1131
TspRI CASTG 1 cut(s) 55
VpaK11BI GGWCC 1 cut(s) 826
XapI RAATTY 2 cut(s) 818, 966
XceI RCATGY 1 cut(s) 792
XspI CTAG 1 cut(s) 642
ZraI GACGTC 2 cut(s) 488, 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.