pycom03g00360

Belongs to the peptidase C2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0029229
Physical Location & Seq
Forward (+)
301726 .. 305424
3699 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g00360.2

Sequence Viewer

Length: 1743 bp
ATGGAAGGAGATGAGCGTCACCTGCTGCTGGCATGCGTGATTTCGGGGACCCTTTTCTCGGTTTTGGGTTCTGCTTCATTTTCTATACTGTGGCTTGTGAATTGGAGGCCCTGGAGGATCTACAGTTGGATCTTTGCCAGGAAATGGCCGGGTATCTTCCATGGACCTCAGCTGGATATAGTATGTGGCTTTCTGTCTCTTTCCGCATGGATACTCGTCATTTCTCCAGTTCTAGTACTGATCATATGGGGAAGCTGGTTGGTTGTAATATTGGATCGACATATAGTTGGTCTGGCTGTAATAATGGCTGGCACTGCTCTTCTACTGTCTTTCTATTCAATCATGCTTTGGTGGCGAACACAATGGCAGAGCTCGAGGGCTGTTGCTATTCTTCTTCTTCTGGCTGTTGCCCTTCTATGTGTGTACGAACTTTGTGCTGTGTATGTTACAGCTGGTTCAAAAGCATCTCAGCAGTATTCGCCGTCTGGTTTCTTTTTTGGCGTCTCAGCAGTTGCCTTGGCAATCAACATGCTCTTTATTTGCAGAATGGTGTTTAATGGGAATGGCTTAGATGTCGATGAATATGTGAGGAAGGCATATAAGTTTGCTTATTCTGATTGTATAGAAGTAGGTCCTGTGGCATGTTTACCAGAACCACCAGACCCTAATGAATTATATCCTCGACAATCTAGCAGGTATGGCTTCACATCTTGGGCTTCTTTACCTTGGCTCACTTGTAGTCCTTCTTGTGTACTCAATCTCCTCTATGGTTTGACTGCAAAGGAGTCACGTTGGCTTGGAGCCATTACATCAGCTGCTGTCATCATTCTTGATTGGAACATGGGTGCGTGCTTGTATGGTTTCGAACTCCTTCAAAGTCGTGTTGCAGCGTTATTTGTTGCTGGAACATCTCGGATCTTTCTCATTTGCTTTGGGGTCCACTACTGGTATTTTGGGCATTGCATTAGTTATGCAGTTGTAGCGTCTGTTTTATTGGGTGCTTCTGTTTCTCGCCATTTATCTGCAACGAACCCGTTGGCTGCAAGGAGAGATGCCTTACGGAGTACCGTGACACGCCTCAGAGAGGGATTTCGCAAAAAAGAGCAAAACAGTTCATCAAGCTCATCTGAAGTTTGTGGTTCAAGCATGAAGCACAGTAGTAGTGTTGAAGCAGGTTGCCTTGGAAATGTGGTTGAAGCAAGTAATAGGAGCACAGCACAGTGCACACTTGATGCCAATAACTGGACTAACACTCTCTTACGAACTGCAAGTTCTCGTGAGGGGATTAATAGTGATAAGAGCATAGATAGTGGAAGGCCAAGTTTAGCATTGCGTAGCAGTTCTTGTCGTTCAGTAATCCAGGAGCCTGAAGTGGGAACATCCTATACTGACAAAAATTTTGATCACAATAACACACTGATGATTTGTTCTAGTAGTGGTCTGGAAAGCCAAGGCTGTGAATCTAGTACGTCAAATTCTGCAAACCAACAAACTTTGGATTTGAATTTAGCTTTTGCATTGCAAGAAAGGTTGAGTGACCCGAGGATTACATCTATGTTAAAGAAGAGGGCAAGACAAGGTGATCTTGAATTGGTTAATCTATTGCAGGATAAAGGATTGGATCCTAACTTTGCTATGATGTTAAAGGAGAAGAGCTTGGATCCAACTATTCTGGCATTACTTCAAAGAAGTAGCTTGGATGCTGAGAGATCGATCACCGTGGCAATACAGATATCACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001558 GO:0001708 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0004198 GO:0005198 GO:0005488 GO:0005509 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005783 GO:0005789 GO:0005829 GO:0005886 GO:0005887 GO:0006275 GO:0006355 GO:0006508 GO:0006807 GO:0006873 GO:0006874 GO:0006875 GO:0006950 GO:0006970 GO:0006996 GO:0007010 GO:0007204 GO:0007275 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0008307 GO:0009628 GO:0009651 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009934 GO:0009966 GO:0009987 GO:0010008 GO:0010035 GO:0010038 GO:0010154 GO:0010468 GO:0010522 GO:0010524 GO:0010556 GO:0010557 GO:0010558 GO:0010564 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010646 GO:0010927 GO:0010941 GO:0010959 GO:0012505 GO:0014717 GO:0014718 GO:0014823 GO:0014850 GO:0016020 GO:0016021 GO:0016043 GO:0016787 GO:0019219 GO:0019222 GO:0019538 GO:0019725 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0030003 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030036 GO:0030154 GO:0030162 GO:0030234 GO:0030239 GO:0030315 GO:0031032 GO:0031090 GO:0031224 GO:0031226 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031402 GO:0031410 GO:0031420 GO:0031432 GO:0031674 GO:0031982 GO:0031984 GO:0032101 GO:0032268 GO:0032269 GO:0032270 GO:0032501 GO:0032502 GO:0032875 GO:0032877 GO:0032879 GO:0032947 GO:0032989 GO:0032991 GO:0033036 GO:0033233 GO:0033234 GO:0033554 GO:0034613 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0040008 GO:0042127 GO:0042175 GO:0042221 GO:0042383 GO:0042592 GO:0042692 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043122 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043269 GO:0043270 GO:0043292 GO:0043415 GO:0043416 GO:0043933 GO:0044085 GO:0044093 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044449 GO:0044459 GO:0044464 GO:0045165 GO:0045214 GO:0045740 GO:0045787 GO:0045862 GO:0045892 GO:0045893 GO:0045927 GO:0045934 GO:0045935 GO:0046872 GO:0048316 GO:0048468 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048583 GO:0048584 GO:0048608 GO:0048638 GO:0048639 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050801 GO:0050865 GO:0050867 GO:0050896 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051146 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051239 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051279 GO:0051281 GO:0051282 GO:0051480 GO:0051592 GO:0051641 GO:0051716 GO:0051726 GO:0051924 GO:0051928 GO:0055001 GO:0055002 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055103 GO:0060255 GO:0060341 GO:0060548 GO:0061041 GO:0061061 GO:0061458 GO:0065003 GO:0065007 GO:0065008 GO:0065009 GO:0070011 GO:0070727 GO:0070887 GO:0070925 GO:0071214 GO:0071241 GO:0071248 GO:0071277 GO:0071470 GO:0071472 GO:0071704 GO:0071840 GO:0071944 GO:0072503 GO:0072507 GO:0072657 GO:0080050 GO:0080090 GO:0080134 GO:0090068 GO:0090303 GO:0090329 GO:0090392 GO:0090627 GO:0090628 GO:0097264 GO:0097435 GO:0097708 GO:0098588 GO:0098771 GO:0098772 GO:0098805 GO:0098827 GO:0099080 GO:0099081 GO:0099512 GO:0104004 GO:0140096 GO:1901564 GO:1902531 GO:1902679 GO:1902680 GO:1903034 GO:1903036 GO:1903169 GO:1903320 GO:1903321 GO:1903506 GO:1903507 GO:1903508 GO:1904062 GO:1904064 GO:1904427 GO:2000011 GO:2000014 GO:2000024 GO:2000026 GO:2000105 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calpain_III Peptidase_C2

Protein Analysis

581

Amino Acids

63.77

Weight (kDa)

8.23

Isoelectric Point (pI)

48.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029229)

Species Orthologous Gene IDs
pyrus_communis pycom03g00360 pycom11g00310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 30
Acc36I ACCTGC 3 cut(s) 30, 684, 1163
AccB7I CCANNNNNTGG 2 cut(s) 144, 1242
AciI CCGC 1 cut(s) 204
AclWI GGATC 8 cut(s) 125, 137, 282, 923, 1616, 1629, 1655, 1668
AcoI YGGCCR 1 cut(s) 146
AcsI RAATTY 3 cut(s) 1396, 1474, 1504
AcuI CTGAAG 2 cut(s) 1149, 1389
AcyI GRCGYC 1 cut(s) 501
AfaI GTAC 5 cut(s) 237, 425, 753, 1066, 1468
AfiI CCNNNNNNNGG 6 cut(s) 28, 58, 144, 1084, 1242, 1373
AgsI TTSAA 9 cut(s) 339, 459, 875, 1143, 1169, 1196, 1504, 1589, 1685
AjnI CCWGG 3 cut(s) 110, 137, 1359
AluBI AGCT 9 cut(s) 172, 255, 372, 452, 815, 1122, 1511, 1656, 1695
AluI AGCT 9 cut(s) 172, 255, 372, 452, 815, 1122, 1511, 1656, 1695
Alw21I GWGCWC 3 cut(s) 374, 1214, 1226
Alw26I GTCTC 2 cut(s) 201, 508
Alw44I GTGCAC 1 cut(s) 1222
AlwI GGATC 8 cut(s) 125, 137, 282, 923, 1616, 1629, 1655, 1668
AlwNI CAGNNNCTG 1 cut(s) 818
Ama87I CYCGRG 2 cut(s) 373, 1540
AoxI GGCC 3 cut(s) 107, 146, 1316
ApaLI GTGCAC 1 cut(s) 1222
ApeKI GCWGC 4 cut(s) 25, 815, 887, 1040
ApoI RAATTY 3 cut(s) 1396, 1474, 1504
ArsI GACNNNNNNTTYG 2 cut(s) 1382, 1414
AseI ATTAAT 1 cut(s) 1287
Asp700I GAANNNNTTC 1 cut(s) 870
AspS9I GGNCC 5 cut(s) 48, 108, 164, 632, 937
AsuC2I CCSGG 1 cut(s) 150
AsuHPI GGTGA 4 cut(s) 11, 1592, 1708, 1728
AsuII TTCGAA 1 cut(s) 864
AvaI CYCGRG 2 cut(s) 373, 1540
AvaII GGWCC 4 cut(s) 48, 164, 632, 937
BaeGI GKGCMC 1 cut(s) 1226
BamHI GGATCC 2 cut(s) 1621, 1660
BanII GRGCYC 1 cut(s) 374
BauI CACGAG 1 cut(s) 1275
Bbv12I GWGCWC 3 cut(s) 374, 1214, 1226
BbvCI CCTCAGC 1 cut(s) 168
BbvI GCAGC 4 cut(s) 12, 802, 899, 1027
BceAI ACGGC 1 cut(s) 466
BcgI CGANNNNNNTGC 2 cut(s) 416, 450
BciT130I CCWGG 3 cut(s) 112, 139, 1361
BciVI GTATCC 1 cut(s) 204
BclI TGATCA 2 cut(s) 240, 1402
BcnI CCSGG 1 cut(s) 150
BcoDI GTCTC 2 cut(s) 201, 508
BfaI CTAG 4 cut(s) 233, 690, 1431, 1464
BfmI CTRYAG 1 cut(s) 121
BfuAI ACCTGC 3 cut(s) 30, 684, 1163
BfuI GTATCC 1 cut(s) 204
BisI GCNGC 4 cut(s) 26, 816, 888, 1041
BlsI GCNGC 4 cut(s) 27, 817, 889, 1042
BmcAI AGTACT 1 cut(s) 237
Bme1390I CCNGG 4 cut(s) 112, 139, 150, 1361
Bme18I GGWCC 4 cut(s) 48, 164, 632, 937
BmeT110I CYCGRG 2 cut(s) 373, 1540
BmgT120I GGNCC 5 cut(s) 48, 108, 164, 632, 937
BmiI GGNNCC 7 cut(s) 49, 50, 802, 938, 1365, 1623, 1662
BmrFI CCNGG 4 cut(s) 112, 139, 150, 1361
BmsI GCATC 4 cut(s) 473, 1042, 1222, 1690
BpmI CTGGAG 2 cut(s) 133, 210
Bpu10I CCTNAGC 1 cut(s) 168
Bpu14I TTCGAA 1 cut(s) 864
BpuMI CCSGG 1 cut(s) 150
Bsa29I ATCGAT 1 cut(s) 1712
BsaHI GRCGYC 1 cut(s) 501
BsaJI CCNNGG 8 cut(s) 110, 160, 516, 725, 1180, 1450, 1541, 1719
BsaXI ACNNNNNCTCC 2 cut(s) 744, 774
Bsc4I CCNNNNNNNGG 6 cut(s) 28, 58, 144, 1084, 1242, 1373
Bse1I ACTGG 3 cut(s) 227, 950, 1247
Bse3DI GCAATG 3 cut(s) 958, 1328, 1517
BseBI CCWGG 3 cut(s) 112, 139, 1361
BseCI ATCGAT 1 cut(s) 1712
BseDI CCNNGG 8 cut(s) 110, 160, 516, 725, 1180, 1450, 1541, 1719
BseGI GGATG 2 cut(s) 1379, 1705
BseLI CCNNNNNNNGG 6 cut(s) 28, 58, 144, 1084, 1242, 1373
BseMI GCAATG 3 cut(s) 958, 1328, 1517
BseMII CTCAG 5 cut(s) 182, 482, 519, 1093, 1695
BseNI ACTGG 3 cut(s) 227, 950, 1247
BseRI GAGGAG 1 cut(s) 752
BseSI GKGCMC 1 cut(s) 1226
BseXI GCAGC 4 cut(s) 12, 802, 899, 1027
BshFI GGCC 3 cut(s) 109, 148, 1318
BshVI ATCGAT 1 cut(s) 1712
BsiHKAI GWGCWC 3 cut(s) 374, 1214, 1226
BsiHKCI CYCGRG 2 cut(s) 373, 1540
BsiSI CCGG 1 cut(s) 149
BslFI GGGAC 1 cut(s) 61
BslI CCNNNNNNNGG 6 cut(s) 28, 58, 144, 1084, 1242, 1373
BsmAI GTCTC 2 cut(s) 201, 508
BsmBI CGTCTC 1 cut(s) 508
BsmFI GGGAC 1 cut(s) 61
BsnI GGCC 3 cut(s) 109, 148, 1318
BsoBI CYCGRG 2 cut(s) 373, 1540
Bsp119I TTCGAA 1 cut(s) 864
Bsp1286I GDGCHC 3 cut(s) 374, 1214, 1226
Bsp19I CCATGG 1 cut(s) 160
BspACI CCGC 1 cut(s) 204
BspANI GGCC 3 cut(s) 109, 148, 1318
BspCNI CTCAG 5 cut(s) 181, 481, 518, 1092, 1696
BspDI ATCGAT 1 cut(s) 1712
BspLI GGNNCC 7 cut(s) 49, 50, 802, 938, 1365, 1623, 1662
BspMI ACCTGC 3 cut(s) 30, 684, 1163
BspPI GGATC 8 cut(s) 125, 137, 282, 923, 1616, 1629, 1655, 1668
BspQI GCTCTTC 2 cut(s) 324, 1646
BspT104I TTCGAA 1 cut(s) 864
BsrDI GCAATG 3 cut(s) 958, 1328, 1517
BsrI ACTGG 3 cut(s) 227, 950, 1247
BssECI CCNNGG 8 cut(s) 110, 160, 516, 725, 1180, 1450, 1541, 1719
BssNI GRCGYC 1 cut(s) 501
BssSI CACGAG 1 cut(s) 1275
BssT1I CCWWGG 5 cut(s) 160, 516, 725, 1180, 1450
Bst2BI CACGAG 1 cut(s) 1275
Bst2UI CCWGG 3 cut(s) 112, 139, 1361
Bst4CI ACNGT 8 cut(s) 90, 125, 327, 1069, 1112, 1157, 1221, 1720
Bst6I CTCTTC 3 cut(s) 324, 1559, 1646
BstACI GRCGYC 1 cut(s) 501
BstBI TTCGAA 1 cut(s) 864
BstC8I GCNNGC 4 cut(s) 30, 34, 310, 850
BstDEI CTNAG 6 cut(s) 168, 468, 505, 568, 1079, 1704
BstDSI CCRYGG 2 cut(s) 160, 1719
BstENI CCTNNNNNAGG 1 cut(s) 1082
BstF5I GGATG 2 cut(s) 1379, 1705
BstMAI GTCTC 2 cut(s) 201, 508
BstMWI GCNNNNNNNGC 6 cut(s) 22, 314, 352, 478, 699, 980
BstNI CCWGG 3 cut(s) 112, 139, 1361
BstNSI RCATGY 3 cut(s) 36, 532, 645
BstSCI CCNGG 4 cut(s) 110, 137, 148, 1359
BstSFI CTRYAG 1 cut(s) 121
BstSLI GKGCMC 1 cut(s) 1226
BstV1I GCAGC 4 cut(s) 12, 802, 899, 1027
BstX2I RGATCY 5 cut(s) 117, 129, 915, 1621, 1660
BstYI RGATCY 5 cut(s) 117, 129, 915, 1621, 1660
Bsu15I ATCGAT 1 cut(s) 1712
BsuI GTATCC 1 cut(s) 204
BsuRI GGCC 3 cut(s) 109, 148, 1318
BsuTUI ATCGAT 1 cut(s) 1712
BtgI CCRYGG 2 cut(s) 160, 1719
BtsCI GGATG 2 cut(s) 1379, 1705
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 3 cut(s) 312, 1226, 1415
BveI ACCTGC 3 cut(s) 30, 684, 1163
Cac8I GCNNGC 4 cut(s) 30, 34, 310, 850
CaiI CAGNNNCTG 1 cut(s) 818
Cfr13I GGNCC 5 cut(s) 48, 108, 164, 632, 937
ClaI ATCGAT 1 cut(s) 1712
CseI GACGC 3 cut(s) 5, 490, 972
Csp6I GTAC 5 cut(s) 236, 424, 752, 1065, 1467
CviAII CATG 8 cut(s) 33, 161, 207, 343, 529, 642, 841, 1147
CviQI GTAC 5 cut(s) 236, 424, 752, 1065, 1467
DdeI CTNAG 6 cut(s) 168, 468, 505, 568, 1079, 1704
EaeI YGGCCR 1 cut(s) 146
Eam1104I CTCTTC 3 cut(s) 324, 1559, 1646
EarI CTCTTC 3 cut(s) 324, 1559, 1646
Ecl136II GAGCTC 1 cut(s) 372
Eco130I CCWWGG 5 cut(s) 160, 516, 725, 1180, 1450
Eco24I GRGCYC 1 cut(s) 374
Eco32I GATATC 1 cut(s) 1734
Eco47I GGWCC 4 cut(s) 48, 164, 632, 937
Eco53kI GAGCTC 1 cut(s) 372
Eco57I CTGAAG 2 cut(s) 1149, 1389
Eco88I CYCGRG 2 cut(s) 373, 1540
EcoICRI GAGCTC 1 cut(s) 372
EcoNI CCTNNNNNAGG 1 cut(s) 1082
EcoO109I RGGNCCY 3 cut(s) 48, 108, 632
EcoRII CCWGG 3 cut(s) 110, 137, 1359
EcoRV GATATC 1 cut(s) 1734
EcoT14I CCWWGG 5 cut(s) 160, 516, 725, 1180, 1450
EcoT38I GRGCYC 1 cut(s) 374
ErhI CCWWGG 5 cut(s) 160, 516, 725, 1180, 1450
Esp3I CGTCTC 1 cut(s) 508
FaeI CATG 8 cut(s) 36, 164, 210, 346, 532, 645, 844, 1150
FalI AAGNNNNNCTT 2 cut(s) 1569, 1601
FaqI GGGAC 1 cut(s) 61
FatI CATG 8 cut(s) 32, 160, 206, 342, 528, 641, 840, 1146
FauNDI CATATG 1 cut(s) 245
FbaI TGATCA 2 cut(s) 240, 1402
Fnu4HI GCNGC 4 cut(s) 26, 816, 888, 1041
FokI GGATG 2 cut(s) 1366, 1712
FriOI GRGCYC 1 cut(s) 374
Fsp4HI GCNGC 4 cut(s) 26, 816, 888, 1041
FspBI CTAG 4 cut(s) 233, 690, 1431, 1464
GluI GCNGC 4 cut(s) 26, 816, 888, 1041
GsuI CTGGAG 2 cut(s) 133, 210
HaeIII GGCC 3 cut(s) 109, 148, 1318
HapII CCGG 1 cut(s) 149
HgaI GACGC 3 cut(s) 5, 490, 972
Hin1I GRCGYC 1 cut(s) 501
Hin1II CATG 8 cut(s) 36, 164, 210, 346, 532, 645, 844, 1150
HinfI GANTC 2 cut(s) 785, 1460
HpaII CCGG 1 cut(s) 149
HphI GGTGA 4 cut(s) 11, 1592, 1708, 1728
Hpy166II GTNNAC 5 cut(s) 424, 647, 752, 940, 1224
Hpy188I TCNGA 4 cut(s) 616, 915, 1082, 1129
Hpy188III TCNNGA 4 cut(s) 830, 1277, 1442, 1586
Hpy8I GTNNAC 5 cut(s) 424, 647, 752, 940, 1224
HpyAV CCTTC 5 cut(s) 422, 586, 753, 881, 1308
HpyCH4III ACNGT 8 cut(s) 90, 125, 327, 1069, 1112, 1157, 1221, 1720
HpyCH4IV ACGT 2 cut(s) 790, 1469
HpyF10VI GCNNNNNNNGC 6 cut(s) 22, 314, 352, 478, 699, 980
HpyF3I CTNAG 6 cut(s) 168, 468, 505, 568, 1079, 1704
HpySE526I ACGT 2 cut(s) 790, 1469
Hsp92I GRCGYC 1 cut(s) 501
Hsp92II CATG 8 cut(s) 36, 164, 210, 346, 532, 645, 844, 1150
KflI GGGWCCC 1 cut(s) 48
Ksp22I TGATCA 2 cut(s) 240, 1402
LguI GCTCTTC 2 cut(s) 324, 1646
LmnI GCTCC 3 cut(s) 800, 1209, 1363
Lsp1109I GCAGC 4 cut(s) 12, 802, 899, 1027
LweI GCATC 4 cut(s) 473, 1042, 1222, 1690
MaeI CTAG 4 cut(s) 233, 690, 1431, 1464
MaeII ACGT 2 cut(s) 790, 1469
MaeIII GTNAC 5 cut(s) 17, 445, 786, 1069, 1535
MboII GAAGA 7 cut(s) 148, 311, 383, 386, 389, 1576, 1663
MflI RGATCY 5 cut(s) 117, 129, 915, 1621, 1660
MhlI GDGCHC 3 cut(s) 374, 1214, 1226
MluCI AATT 6 cut(s) 100, 671, 1396, 1474, 1504, 1589
MlyI GAGTC 1 cut(s) 794
MmeI TCCRAC 2 cut(s) 107, 1688
MroXI GAANNNNTTC 1 cut(s) 870
MseI TTAA 5 cut(s) 555, 1287, 1559, 1596, 1643
MslI CAYNNNNRTG 1 cut(s) 1418
MspA1I CMGCKG 3 cut(s) 172, 452, 815
MspI CCGG 1 cut(s) 149
MspR9I CCNGG 4 cut(s) 112, 139, 150, 1361
MvaI CCWGG 3 cut(s) 112, 139, 1361
MwoI GCNNNNNNNGC 6 cut(s) 22, 314, 352, 478, 699, 980
NciI CCSGG 1 cut(s) 150
NcoI CCATGG 1 cut(s) 160
NdeI CATATG 1 cut(s) 245
NlaIII CATG 8 cut(s) 36, 164, 210, 346, 532, 645, 844, 1150
NlaIV GGNNCC 7 cut(s) 49, 50, 802, 938, 1365, 1623, 1662
NmuCI GTSAC 4 cut(s) 17, 786, 1069, 1535
NspI RCATGY 3 cut(s) 36, 532, 645
NspV TTCGAA 1 cut(s) 864
PaeI GCATGC 1 cut(s) 36
PaeR7I CTCGAG 1 cut(s) 373
PaqCI CACCTGC 1 cut(s) 30
PciSI GCTCTTC 2 cut(s) 324, 1646
PdmI GAANNNNTTC 1 cut(s) 870
PfeI GAWTC 1 cut(s) 1460
PflMI CCANNNNNTGG 2 cut(s) 144, 1242
PfoI TCCNGGA 1 cut(s) 1359
PkrI GCNGC 4 cut(s) 27, 817, 889, 1042
PleI GAGTC 1 cut(s) 793
PpsI GAGTC 1 cut(s) 793
PpuMI RGGWCCY 2 cut(s) 48, 632
PshBI ATTAAT 1 cut(s) 1287
Psp124BI GAGCTC 1 cut(s) 374
Psp5II RGGWCCY 2 cut(s) 48, 632
Psp6I CCWGG 3 cut(s) 110, 137, 1359
PspGI CCWGG 3 cut(s) 110, 137, 1359
PspN4I GGNNCC 7 cut(s) 49, 50, 802, 938, 1365, 1623, 1662
PspPI GGNCC 5 cut(s) 48, 108, 164, 632, 937
PspPPI RGGWCCY 2 cut(s) 48, 632
PspXI VCTCGAGB 1 cut(s) 373
PsrI GAACNNNNNNTAC 2 cut(s) 1369, 1401
PstNI CAGNNNCTG 1 cut(s) 818
PsuI RGATCY 5 cut(s) 117, 129, 915, 1621, 1660
PvuII CAGCTG 3 cut(s) 172, 452, 815
RsaI GTAC 5 cut(s) 237, 425, 753, 1066, 1468
RsaNI GTAC 5 cut(s) 236, 424, 752, 1065, 1467
RseI CAYNNNNRTG 1 cut(s) 1418
SacI GAGCTC 1 cut(s) 374
SapI GCTCTTC 2 cut(s) 324, 1646
SaqAI TTAA 5 cut(s) 555, 1287, 1559, 1596, 1643
SatI GCNGC 4 cut(s) 26, 816, 888, 1041
Sau96I GGNCC 5 cut(s) 48, 108, 164, 632, 937
ScaI AGTACT 1 cut(s) 237
SchI GAGTC 1 cut(s) 794
ScrFI CCNGG 4 cut(s) 112, 139, 150, 1361
SduI GDGCHC 3 cut(s) 374, 1214, 1226
SfaNI GCATC 4 cut(s) 473, 1042, 1222, 1690
SfcI CTRYAG 1 cut(s) 121
Sfr274I CTCGAG 1 cut(s) 373
SfuI TTCGAA 1 cut(s) 864
SinI GGWCC 4 cut(s) 48, 164, 632, 937
SlaI CTCGAG 1 cut(s) 373
SmiMI CAYNNNNRTG 1 cut(s) 1418
SmlI CTYRAG 1 cut(s) 373
SmoI CTYRAG 1 cut(s) 373
SphI GCATGC 1 cut(s) 36
Sse9I AATT 6 cut(s) 100, 671, 1396, 1474, 1504, 1589
SsiI CCGC 1 cut(s) 204
SspI AATATT 1 cut(s) 270
SspMI CTAG 4 cut(s) 233, 690, 1431, 1464
SstI GAGCTC 1 cut(s) 374
StyD4I CCNGG 4 cut(s) 110, 137, 148, 1359
StyI CCWWGG 5 cut(s) 160, 516, 725, 1180, 1450
TaaI ACNGT 8 cut(s) 90, 125, 327, 1069, 1112, 1157, 1221, 1720
TaiI ACGT 2 cut(s) 793, 1472
TaqI TCGA 6 cut(s) 277, 374, 576, 682, 864, 1712
TasI AATT 6 cut(s) 100, 671, 1396, 1474, 1504, 1589
TatI WGTACW 2 cut(s) 235, 751
TfiI GAWTC 1 cut(s) 1460
Tru1I TTAA 5 cut(s) 555, 1287, 1559, 1596, 1643
Tru9I TTAA 5 cut(s) 555, 1287, 1559, 1596, 1643
TscAI CASTG 3 cut(s) 319, 1226, 1422
TseFI GTSAC 4 cut(s) 17, 786, 1069, 1535
TseI GCWGC 4 cut(s) 25, 815, 887, 1040
Tsp45I GTSAC 4 cut(s) 17, 786, 1069, 1535
TspDTI ATGAA 5 cut(s) 66, 594, 684, 1104, 1163
TspGWI ACGGA 1 cut(s) 1075
TspRI CASTG 3 cut(s) 319, 1226, 1422
Van91I CCANNNNNTGG 2 cut(s) 144, 1242
VneI GTGCAC 1 cut(s) 1222
VpaK11BI GGWCC 4 cut(s) 48, 164, 632, 937
VspI ATTAAT 1 cut(s) 1287
XagI CCTNNNNNAGG 1 cut(s) 1082
XapI RAATTY 3 cut(s) 1396, 1474, 1504
XceI RCATGY 3 cut(s) 36, 532, 645
XhoI CTCGAG 1 cut(s) 373
XmnI GAANNNNTTC 1 cut(s) 870
XspI CTAG 4 cut(s) 233, 690, 1431, 1464
ZrmI AGTACT 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.