pycom03g01940

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0023543
Physical Location & Seq
Reverse (-)
1457239 .. 1458556
1318 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 537 bp
ATGATTTTGCACTTTCTAGCAGTTGGCCTCTGCCATTTTCCATCCAATCCAAATCAAAATCTCTCCAATTTTCCCGAGAAACAAACGCTTCCTAAACCCTTCAGTCTTCCTCTTTCATCTTCCCAAATGGCTCGTACCAAGCAGACTGCACGCAAGTCCACCGGAGGAAAGGCACCAAGGAAGCAGCTGGCAACAAAGGCTGCTCGGAAATCTGCTCCCGCGACCGGAGGAGTGAAGAAGCCTCACCGATTCAGGCCGGGAACCGTGGCCCTGAGGGAGATCAGGAAGTACCAGAAGAGCACTGAGCTCCTGATCCGCAAGCTGCCGTTCCAGAGGCTGGTGAGGGAGATCGCACAGGACTTCAAGACCGATTTGAGGTTCCAGAGCAGCGCTGTATCGGCGCTCCAGGAGGCGGCAGAGGCGTACTTGGTGGGGCTGTTTGAGGACACCAATCTGTGTGCCATTCATGCCAAGAGGGTCACCATTATGCCCAAGGACATCCAGCTTGCTCGTAGGATTAGGGGCGAGAGGGCTTAA

Protein Analysis

179

Amino Acids

19.95

Weight (kDa)

11.02

Isoelectric Point (pI)

46.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 86 - 173 1.4e-38 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023543)

Species Orthologous Gene IDs
malus_domestica MD11G1027400.v1.1 MD11G1027600.v1.1
pyrus_communis pycom03g01940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 172
AccB7I CCANNNNNTGG 1 cut(s) 337
AccII CGCG 1 cut(s) 221
AciI CCGC 3 cut(s) 219, 316, 413
AclWI GGATC 1 cut(s) 307
AcuI CTGAAG 1 cut(s) 85
AfaI GTAC 3 cut(s) 136, 290, 425
AfeI AGCGCT 1 cut(s) 391
AfiI CCNNNNNNNGG 4 cut(s) 224, 337, 375, 412
AgsI TTSAA 1 cut(s) 364
AjnI CCWGG 1 cut(s) 405
AluBI AGCT 4 cut(s) 187, 307, 322, 505
AluI AGCT 4 cut(s) 187, 307, 322, 505
Alw21I GWGCWC 2 cut(s) 302, 309
AlwI GGATC 1 cut(s) 307
AlwNI CAGNNNCTG 1 cut(s) 337
Ama87I CYCGRG 1 cut(s) 74
Aor51HI AGCGCT 1 cut(s) 391
AoxI GGCC 3 cut(s) 25, 254, 267
ApeKI GCWGC 4 cut(s) 184, 200, 322, 387
AspLEI GCGC 2 cut(s) 392, 403
AspS9I GGNCC 1 cut(s) 268
AsuC2I CCSGG 1 cut(s) 258
AsuHPI GGTGA 3 cut(s) 236, 352, 472
AvaI CYCGRG 1 cut(s) 74
AxyI CCTNAGG 1 cut(s) 272
BanI GGYRCC 1 cut(s) 172
BanII GRGCYC 1 cut(s) 309
BbsI GAAGAC 1 cut(s) 98
Bbv12I GWGCWC 2 cut(s) 302, 309
BbvI GCAGC 4 cut(s) 187, 196, 309, 399
BccI CCATC 1 cut(s) 49
BceAI ACGGC 1 cut(s) 310
BciT130I CCWGG 1 cut(s) 407
BcnI CCSGG 1 cut(s) 258
BfaI CTAG 1 cut(s) 17
BfoI RGCGCY 2 cut(s) 393, 404
BisI GCNGC 5 cut(s) 185, 201, 323, 388, 414
BlsI GCNGC 5 cut(s) 186, 202, 324, 389, 415
Bme1390I CCNGG 2 cut(s) 258, 407
BmeT110I CYCGRG 1 cut(s) 74
BmgT120I GGNCC 1 cut(s) 268
BmiI GGNNCC 3 cut(s) 174, 262, 380
BmrFI CCNGG 2 cut(s) 258, 407
BpiI GAAGAC 1 cut(s) 98
BpmI CTGGAG 1 cut(s) 389
BpuMI CCSGG 1 cut(s) 258
BsaJI CCNNGG 3 cut(s) 176, 264, 492
BsaWI WCCGGW 2 cut(s) 161, 224
Bsc4I CCNNNNNNNGG 4 cut(s) 224, 337, 375, 412
Bse21I CCTNAGG 1 cut(s) 272
BseBI CCWGG 1 cut(s) 407
BseDI CCNNGG 3 cut(s) 176, 264, 492
BseGI GGATG 2 cut(s) 41, 498
BseLI CCNNNNNNNGG 4 cut(s) 224, 337, 375, 412
BseMII CTCAG 2 cut(s) 263, 294
BseRI GAGGAG 1 cut(s) 243
BseXI GCAGC 4 cut(s) 187, 196, 309, 399
BsgI GTGCAG 1 cut(s) 132
Bsh1236I CGCG 1 cut(s) 221
Bsh1285I CGRYCG 1 cut(s) 225
BshFI GGCC 3 cut(s) 27, 256, 269
BshNI GGYRCC 1 cut(s) 172
BsiEI CGRYCG 1 cut(s) 225
BsiHKAI GWGCWC 2 cut(s) 302, 309
BsiHKCI CYCGRG 1 cut(s) 74
BsiSI CCGG 3 cut(s) 162, 225, 257
BslI CCNNNNNNNGG 4 cut(s) 224, 337, 375, 412
BsnI GGCC 3 cut(s) 27, 256, 269
BsoBI CYCGRG 1 cut(s) 74
Bsp1286I GDGCHC 2 cut(s) 302, 309
Bsp143I GATC 3 cut(s) 279, 312, 348
BspACI CCGC 3 cut(s) 219, 316, 413
BspANI GGCC 3 cut(s) 27, 256, 269
BspCNI CTCAG 2 cut(s) 264, 295
BspFNI CGCG 1 cut(s) 221
BspLI GGNNCC 3 cut(s) 174, 262, 380
BspPI GGATC 1 cut(s) 307
BspQI GCTCTTC 1 cut(s) 290
BspT107I GGYRCC 1 cut(s) 172
BssECI CCNNGG 3 cut(s) 176, 264, 492
BssMI GATC 3 cut(s) 279, 312, 348
BssT1I CCWWGG 2 cut(s) 176, 492
Bst2UI CCWGG 1 cut(s) 407
Bst4CI ACNGT 1 cut(s) 265
Bst6I CTCTTC 1 cut(s) 290
BstC8I GCNNGC 4 cut(s) 151, 189, 320, 507
BstDEI CTNAG 2 cut(s) 272, 303
BstDSI CCRYGG 1 cut(s) 264
BstEII GGTNACC 1 cut(s) 478
BstF5I GGATG 2 cut(s) 41, 498
BstFNI CGCG 1 cut(s) 221
BstH2I RGCGCY 2 cut(s) 393, 404
BstHHI GCGC 2 cut(s) 392, 403
BstKTI GATC 3 cut(s) 282, 315, 351
BstMBI GATC 3 cut(s) 279, 312, 348
BstMCI CGRYCG 1 cut(s) 225
BstMWI GCNNNNNNNGC 4 cut(s) 197, 398, 419, 467
BstNI CCWGG 1 cut(s) 407
BstPI GGTNACC 1 cut(s) 478
BstSCI CCNGG 2 cut(s) 256, 405
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 4 cut(s) 187, 196, 309, 399
BstV2I GAAGAC 1 cut(s) 98
Bsu36I CCTNAGG 1 cut(s) 272
BsuRI GGCC 3 cut(s) 27, 256, 269
BtgI CCRYGG 1 cut(s) 264
BtsCI GGATG 2 cut(s) 41, 498
BtsIMutI CAGTG 1 cut(s) 300
Cac8I GCNNGC 4 cut(s) 151, 189, 320, 507
CaiI CAGNNNCTG 1 cut(s) 337
CfoI GCGC 2 cut(s) 392, 403
Cfr13I GGNCC 1 cut(s) 268
Csp6I GTAC 3 cut(s) 135, 289, 424
CviAII CATG 1 cut(s) 467
CviQI GTAC 3 cut(s) 135, 289, 424
DdeI CTNAG 2 cut(s) 272, 303
DpnI GATC 3 cut(s) 281, 314, 350
DpnII GATC 3 cut(s) 279, 312, 348
Eam1104I CTCTTC 1 cut(s) 290
EarI CTCTTC 1 cut(s) 290
Ecl136II GAGCTC 1 cut(s) 307
Eco130I CCWWGG 2 cut(s) 176, 492
Eco24I GRGCYC 1 cut(s) 309
Eco47III AGCGCT 1 cut(s) 391
Eco53kI GAGCTC 1 cut(s) 307
Eco57I CTGAAG 1 cut(s) 85
Eco81I CCTNAGG 1 cut(s) 272
Eco88I CYCGRG 1 cut(s) 74
Eco91I GGTNACC 1 cut(s) 478
EcoICRI GAGCTC 1 cut(s) 307
EcoO65I GGTNACC 1 cut(s) 478
EcoRII CCWGG 1 cut(s) 405
EcoT14I CCWWGG 2 cut(s) 176, 492
EcoT38I GRGCYC 1 cut(s) 309
ErhI CCWWGG 2 cut(s) 176, 492
FaeI CATG 1 cut(s) 470
FaiI YATR 2 cut(s) 468, 488
FatI CATG 1 cut(s) 466
FauI CCCGC 1 cut(s) 226
Fnu4HI GCNGC 5 cut(s) 185, 201, 323, 388, 414
FokI GGATG 2 cut(s) 28, 485
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 5 cut(s) 185, 201, 323, 388, 414
FspBI CTAG 1 cut(s) 17
GlaI GCGC 2 cut(s) 391, 402
GluI GCNGC 5 cut(s) 185, 201, 323, 388, 414
GsuI CTGGAG 1 cut(s) 389
HaeII RGCGCY 2 cut(s) 393, 404
HaeIII GGCC 3 cut(s) 27, 256, 269
HapII CCGG 3 cut(s) 162, 225, 257
HhaI GCGC 2 cut(s) 392, 403
Hin1II CATG 1 cut(s) 470
Hin6I GCGC 2 cut(s) 390, 401
HinP1I GCGC 2 cut(s) 390, 401
HinfI GANTC 1 cut(s) 249
HpaII CCGG 3 cut(s) 162, 225, 257
HphI GGTGA 3 cut(s) 236, 352, 472
Hpy166II GTNNAC 1 cut(s) 159
Hpy188I TCNGA 1 cut(s) 207
Hpy188III TCNNGA 6 cut(s) 74, 283, 310, 331, 364, 382
Hpy8I GTNNAC 1 cut(s) 159
HpyAV CCTTC 1 cut(s) 109
HpyCH4III ACNGT 1 cut(s) 265
HpyCH4V TGCA 2 cut(s) 10, 149
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 398, 419, 467
HpyF3I CTNAG 2 cut(s) 272, 303
Hsp92II CATG 1 cut(s) 470
HspAI GCGC 2 cut(s) 390, 401
Kzo9I GATC 3 cut(s) 279, 312, 348
LguI GCTCTTC 1 cut(s) 290
LmnI GCTCC 3 cut(s) 220, 312, 408
Lsp1109I GCAGC 4 cut(s) 187, 196, 309, 399
MaeI CTAG 1 cut(s) 17
MaeIII GTNAC 1 cut(s) 478
MalI GATC 3 cut(s) 281, 314, 350
MboI GATC 3 cut(s) 279, 312, 348
MboII GAAGA 4 cut(s) 98, 111, 247, 307
MhlI GDGCHC 2 cut(s) 302, 309
MluCI AATT 1 cut(s) 67
MseI TTAA 1 cut(s) 535
MslI CAYNNNNRTG 1 cut(s) 485
MspA1I CMGCKG 1 cut(s) 187
MspI CCGG 3 cut(s) 162, 225, 257
MspR9I CCNGG 2 cut(s) 258, 407
MvaI CCWGG 1 cut(s) 407
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 4 cut(s) 197, 398, 419, 467
NciI CCSGG 1 cut(s) 258
NdeII GATC 3 cut(s) 279, 312, 348
NlaIII CATG 1 cut(s) 470
NlaIV GGNNCC 3 cut(s) 174, 262, 380
NmuCI GTSAC 1 cut(s) 478
PciSI GCTCTTC 1 cut(s) 290
PfeI GAWTC 1 cut(s) 249
PflMI CCANNNNNTGG 1 cut(s) 337
PfoI TCCNGGA 1 cut(s) 405
PkrI GCNGC 5 cut(s) 186, 202, 324, 389, 415
Psp124BI GAGCTC 1 cut(s) 309
Psp6I CCWGG 1 cut(s) 405
PspEI GGTNACC 1 cut(s) 478
PspGI CCWGG 1 cut(s) 405
PspN4I GGNNCC 3 cut(s) 174, 262, 380
PspPI GGNCC 1 cut(s) 268
PstNI CAGNNNCTG 1 cut(s) 337
PvuII CAGCTG 1 cut(s) 187
RsaI GTAC 3 cut(s) 136, 290, 425
RsaNI GTAC 3 cut(s) 135, 289, 424
RseI CAYNNNNRTG 1 cut(s) 485
SacI GAGCTC 1 cut(s) 309
SapI GCTCTTC 1 cut(s) 290
SaqAI TTAA 1 cut(s) 535
SatI GCNGC 5 cut(s) 185, 201, 323, 388, 414
Sau3AI GATC 3 cut(s) 279, 312, 348
Sau96I GGNCC 1 cut(s) 268
ScrFI CCNGG 2 cut(s) 258, 407
SduI GDGCHC 2 cut(s) 302, 309
SetI ASST 5 cut(s) 189, 309, 324, 380, 507
SmiMI CAYNNNNRTG 1 cut(s) 485
Sse9I AATT 1 cut(s) 67
SsiI CCGC 3 cut(s) 219, 316, 413
SspMI CTAG 1 cut(s) 17
SstI GAGCTC 1 cut(s) 309
StyD4I CCNGG 2 cut(s) 256, 405
StyI CCWWGG 2 cut(s) 176, 492
TaaI ACNGT 1 cut(s) 265
TaqII GACCGA 1 cut(s) 383
TasI AATT 1 cut(s) 67
TauI GCSGC 1 cut(s) 416
TfiI GAWTC 1 cut(s) 249
Tru1I TTAA 1 cut(s) 535
Tru9I TTAA 1 cut(s) 535
TscAI CASTG 1 cut(s) 307
TseFI GTSAC 1 cut(s) 478
TseI GCWGC 4 cut(s) 184, 200, 322, 387
Tsp45I GTSAC 1 cut(s) 478
TspDTI ATGAA 2 cut(s) 105, 455
TspRI CASTG 1 cut(s) 307
Van91I CCANNNNNTGG 1 cut(s) 337
XspI CTAG 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.