pycom03g02620

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0021229
Physical Location & Seq
Reverse (-)
2000872 .. 2003076
2205 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1497 bp
ATGGATGCTGGCGATTGGAGGAGTCAGCTGCAGGCTGATTCAAGGCAAAGAATTGTCAACAAGATAATGGATACATTGAAGAGGCATCTTCCCTTTAACGGACAAGATGGATTGCAGGAACTCAGCAAAATTGCTGTAAGGTTTGAGGAAAAGATTTATACTGCAGCCACAAGCCAGTCGGATTATCTACGGAAAATTTCTCTGAAGATGCTCACAATGGAGACCAAGTCTCAGAATTCAACGGGCAATCCTTTACAATCTAACTCTGCTGGTAACAGCAACAGGCCCCCTGATCCAGTCCCCAATCAAGGTATGCAACCCCAAGTCCCCAATCATGGGCAATCACTCTCTATGCCGTTGCCAGCTAATCAATCTCAAGGACGCCAACCACTTTTATCACAGAACATTCAGAATAACATTCCGCCTTCTGGAGTTCAAAGTTCATCTGGCTTATCATCCGCACTACCTCCTTCTTCCGGTTTAACCCAGACTCCTATCCCAAGTATTGTTGGACAAAACCCAAACATGCAAAATATGTCCGGAAGTTCACAGAATTCATTAGGGAATTCCATGGGCCAGGGGGTACCCTCCAATGTCTTTACTAATTCTCAAAGGCAAATGCCAGGAAGGCAACAGGTAGTTCCCCAACAGCAGCAGCCGCAGCAATCCCAGAATTCCCAGAATTCCCAGCAGTATATATACCATCAACAGATGCAACATTCGCTTTTGAAGCCAAAATATCAGCAGGGAAATATGCCACAACTTGTGCAACAGCAACAGCAGCAGAACCTTTTACAATCGACTCCGTTGCAGTCTTCCCAGCAATCTGTTATGCCAACATCATCTGTTATGCAGACTTCGGTGCAACCATCTCTCTCGAGTCTTCAGCAGAATCAACAATCTGCTATGCAACAGTCATCACAACCCATGCTTCAGCAGCATTCACAGCCAGTTCTCAGGCAGCAACAGCAGCCTCAACAGGCAGCTGTTCATCAACAGCAAACACCAATGGCACAACAGCCAATATTACCCCCGCAGCAGCAGCAGCCGCCGCTTATGGTGCAACAGCCAACTGCTTCAAACTTGTCCCAGAATCAGATGATTGGACAACAAAATAATGTTGGGGATATGCAGCAGCAGCAACAACAGAGGTTACTAAACCAGCAGAATAATCTTTTAAACCTTCAACAACAGCAACAGCAGCAGCAGCAGCAGCAGCAGTTAATGGCTCAGCAAAGCAACCTTTCTAATATCCATCAGCAACAATTGGGCCCGCAGAGTAATGTTACTGGGTTACAGCAACAGCAGCAAAACAACCTTTCAAATATGCATCAGCAGCAGTTGGGCTCTCAGAGTAATGTTACCGGATTACAGCAACAGCAGCACCTTGGAACTCAAAGTGGTAATTCTAGTATGCAACCAAATCAGCACTCTGTACACTTGTTGCAACAATCCAAGGACAGCAGTCACAACCGCAAGCATCACAGCAACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

499

Amino Acids

55.49

Weight (kDa)

10.24

Isoelectric Point (pI)

90.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 4 - 83 4.3e-35 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021229)

Species Orthologous Gene IDs
prunus_persica Prupe.6G111000_v2.0.a1 Prupe.6G111000_v2.0.a1 Prupe.6G111000_v2.0.a1 Prupe.6G111000_v2.0.a1
pyrus_communis pycom03g02620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 583
AccB1I GGYRCC 1 cut(s) 583
AccIII TCCGGA 1 cut(s) 539
AciI CCGC 8 cut(s) 422, 459, 659, 1034, 1049, 1052, 1274, 1474
AclWI GGATC 1 cut(s) 287
AcsI RAATTY 6 cut(s) 195, 235, 553, 565, 673, 682
AcuI CTGAAG 3 cut(s) 224, 869, 917
AcyI GRCGYC 1 cut(s) 382
AfaI GTAC 2 cut(s) 585, 1437
AfiI CCNNNNNNNGG 6 cut(s) 98, 308, 335, 336, 428, 476
AgsI TTSAA 8 cut(s) 42, 79, 240, 437, 730, 1080, 1187, 1323
AjnI CCWGG 2 cut(s) 576, 622
AluBI AGCT 3 cut(s) 28, 365, 986
AluI AGCT 3 cut(s) 28, 365, 986
Alw26I GTCTC 2 cut(s) 215, 234
AlwI GGATC 1 cut(s) 287
Ama87I CYCGRG 1 cut(s) 877
Aor13HI TCCGGA 1 cut(s) 539
AoxI GGCC 3 cut(s) 284, 574, 1270
ApaI GGGCCC 1 cut(s) 1274
ApoI RAATTY 6 cut(s) 195, 235, 553, 565, 673, 682
Asp718I GGTACC 1 cut(s) 583
AspS9I GGNCC 4 cut(s) 285, 574, 1270, 1271
AvaI CYCGRG 1 cut(s) 877
BaeGI GKGCMC 1 cut(s) 1274
BanI GGYRCC 1 cut(s) 583
BanII GRGCYC 2 cut(s) 1274, 1349
BbsI GAAGAC 2 cut(s) 807, 875
BccI CCATC 4 cut(s) 101, 711, 877, 1263
BceAI ACGGC 1 cut(s) 340
BcgI CGANNNNNNTGC 2 cut(s) 790, 824
BciT130I CCWGG 2 cut(s) 578, 624
BciVI GTATCC 1 cut(s) 64
BcoDI GTCTC 2 cut(s) 215, 234
BfaI CTAG 1 cut(s) 1410
BfmI CTRYAG 2 cut(s) 29, 162
BfuI GTATCC 1 cut(s) 64
BglI GCCNNNNNGGC 1 cut(s) 628
BlpI GCTNAGC 1 cut(s) 1230
Bme1390I CCNGG 2 cut(s) 578, 624
BmeT110I CYCGRG 1 cut(s) 877
BmgT120I GGNCC 4 cut(s) 285, 574, 1270, 1271
BmiI GGNNCC 3 cut(s) 287, 585, 1272
BmrFI CCNGG 2 cut(s) 578, 624
BmrI ACTGGG 1 cut(s) 1299
BmsI GCATC 5 cut(s) 94, 198, 702, 1339, 1489
BmuI ACTGGG 1 cut(s) 1299
BoxI GACNNNNGTC 1 cut(s) 1464
BpiI GAAGAC 2 cut(s) 807, 875
BpmI CTGGAG 1 cut(s) 450
Bpu1102I GCTNAGC 1 cut(s) 1230
BpuEI CTTGAG 1 cut(s) 360
BsaHI GRCGYC 1 cut(s) 382
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 4 cut(s) 570, 577, 1387, 1455
BsaWI WCCGGW 3 cut(s) 476, 539, 1364
BsaXI ACNNNNNCTCC 4 cut(s) 212, 242, 475, 505
Bsc4I CCNNNNNNNGG 6 cut(s) 98, 308, 335, 336, 428, 476
Bse1I ACTGG 4 cut(s) 175, 296, 950, 1294
BseAI TCCGGA 1 cut(s) 539
BseBI CCWGG 2 cut(s) 578, 624
BseDI CCNNGG 4 cut(s) 570, 577, 1387, 1455
BseGI GGATG 2 cut(s) 10, 455
BseLI CCNNNNNNNGG 6 cut(s) 98, 308, 335, 336, 428, 476
BseMII CTCAG 5 cut(s) 136, 245, 970, 1244, 1364
BseNI ACTGG 4 cut(s) 175, 296, 950, 1294
BseRI GAGGAG 1 cut(s) 34
BseSI GKGCMC 1 cut(s) 1274
BseYI CCCAGC 2 cut(s) 687, 819
BshFI GGCC 3 cut(s) 286, 576, 1272
BshNI GGYRCC 1 cut(s) 583
BsiHKCI CYCGRG 1 cut(s) 877
BsiSI CCGG 3 cut(s) 477, 540, 1365
BslFI GGGAC 3 cut(s) 284, 311, 1072
BslI CCNNNNNNNGG 6 cut(s) 98, 308, 335, 336, 428, 476
BsmAI GTCTC 2 cut(s) 215, 234
BsmFI GGGAC 3 cut(s) 284, 311, 1072
BsmI GAATGC 1 cut(s) 940
BsnI GGCC 3 cut(s) 286, 576, 1272
Bso31I GGTCTC 1 cut(s) 215
BsoBI CYCGRG 1 cut(s) 877
Bsp120I GGGCCC 1 cut(s) 1270
Bsp1286I GDGCHC 2 cut(s) 1274, 1349
Bsp13I TCCGGA 1 cut(s) 539
Bsp1407I TGTACA 1 cut(s) 1435
Bsp143I GATC 1 cut(s) 292
Bsp1720I GCTNAGC 1 cut(s) 1230
Bsp19I CCATGG 1 cut(s) 570
BspACI CCGC 8 cut(s) 422, 459, 659, 1034, 1049, 1052, 1274, 1474
BspANI GGCC 3 cut(s) 286, 576, 1272
BspCNI CTCAG 5 cut(s) 135, 244, 969, 1243, 1363
BspEI TCCGGA 1 cut(s) 539
BspLI GGNNCC 3 cut(s) 287, 585, 1272
BspMAI CTGCAG 2 cut(s) 33, 166
BspPI GGATC 1 cut(s) 287
BspT107I GGYRCC 1 cut(s) 583
BspTNI GGTCTC 1 cut(s) 215
BsrGI TGTACA 1 cut(s) 1435
BsrI ACTGG 4 cut(s) 175, 296, 950, 1294
BssECI CCNNGG 4 cut(s) 570, 577, 1387, 1455
BssMI GATC 1 cut(s) 292
BssNI GRCGYC 1 cut(s) 382
BssT1I CCWWGG 3 cut(s) 570, 1387, 1455
Bst2UI CCWGG 2 cut(s) 578, 624
Bst4CI ACNGT 1 cut(s) 915
Bst6I CTCTTC 1 cut(s) 74
BstACI GRCGYC 1 cut(s) 382
BstAUI TGTACA 1 cut(s) 1435
BstC8I GCNNGC 5 cut(s) 10, 33, 363, 1274, 1478
BstDEI CTNAG 5 cut(s) 122, 231, 956, 1230, 1350
BstDSI CCRYGG 1 cut(s) 570
BstF5I GGATG 2 cut(s) 10, 455
BstKTI GATC 1 cut(s) 295
BstMAI GTCTC 2 cut(s) 215, 234
BstMBI GATC 1 cut(s) 292
BstNI CCWGG 2 cut(s) 578, 624
BstNSI RCATGY 1 cut(s) 529
BstPAI GACNNNNGTC 1 cut(s) 1464
BstSCI CCNGG 2 cut(s) 576, 622
BstSFI CTRYAG 2 cut(s) 29, 162
BstSLI GKGCMC 1 cut(s) 1274
BstV2I GAAGAC 2 cut(s) 807, 875
BsuI GTATCC 1 cut(s) 64
BsuRI GGCC 3 cut(s) 286, 576, 1272
BtgI CCRYGG 1 cut(s) 570
BtsCI GGATG 2 cut(s) 10, 455
Cac8I GCNNGC 5 cut(s) 10, 33, 363, 1274, 1478
Cfr13I GGNCC 4 cut(s) 285, 574, 1270, 1271
CseI GACGC 1 cut(s) 390
Csp6I GTAC 2 cut(s) 584, 1436
CviAII CATG 4 cut(s) 335, 526, 571, 928
CviQI GTAC 2 cut(s) 584, 1436
DdeI CTNAG 5 cut(s) 122, 231, 956, 1230, 1350
DpnI GATC 1 cut(s) 294
DpnII GATC 1 cut(s) 292
DraI TTTAAA 1 cut(s) 1179
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
EciI GGCGGA 1 cut(s) 411
Eco130I CCWWGG 3 cut(s) 570, 1387, 1455
Eco24I GRGCYC 2 cut(s) 1274, 1349
Eco31I GGTCTC 1 cut(s) 215
Eco57I CTGAAG 3 cut(s) 224, 869, 917
Eco88I CYCGRG 1 cut(s) 877
EcoO109I RGGNCCY 1 cut(s) 285
EcoRI GAATTC 5 cut(s) 235, 553, 565, 673, 682
EcoRII CCWGG 2 cut(s) 576, 622
EcoT14I CCWWGG 3 cut(s) 570, 1387, 1455
EcoT22I ATGCAT 1 cut(s) 1332
EcoT38I GRGCYC 2 cut(s) 1274, 1349
ErhI CCWWGG 3 cut(s) 570, 1387, 1455
FaeI CATG 4 cut(s) 338, 529, 574, 931
FaqI GGGAC 3 cut(s) 284, 311, 1072
FatI CATG 4 cut(s) 334, 525, 570, 927
FauI CCCGC 2 cut(s) 1041, 1281
FokI GGATG 2 cut(s) 17, 442
FriOI GRGCYC 2 cut(s) 1274, 1349
FspBI CTAG 1 cut(s) 1410
GsaI CCCAGC 2 cut(s) 691, 823
GsuI CTGGAG 1 cut(s) 450
HaeIII GGCC 3 cut(s) 286, 576, 1272
HapII CCGG 3 cut(s) 477, 540, 1365
HgaI GACGC 1 cut(s) 390
Hin1I GRCGYC 1 cut(s) 382
Hin1II CATG 4 cut(s) 338, 529, 574, 931
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HinfI GANTC 7 cut(s) 22, 38, 490, 802, 880, 892, 1093
HpaII CCGG 3 cut(s) 477, 540, 1365
Hpy166II GTNNAC 3 cut(s) 58, 548, 1438
Hpy188I TCNGA 6 cut(s) 181, 204, 234, 411, 1098, 1353
Hpy188III TCNNGA 3 cut(s) 429, 540, 877
Hpy8I GTNNAC 3 cut(s) 58, 548, 1438
HpyAV CCTTC 4 cut(s) 435, 480, 621, 1193
HpyCH4III ACNGT 1 cut(s) 915
HpyF3I CTNAG 5 cut(s) 122, 231, 956, 1230, 1350
Hsp92I GRCGYC 1 cut(s) 382
Hsp92II CATG 4 cut(s) 338, 529, 574, 931
Kpn2I TCCGGA 1 cut(s) 539
KpnI GGTACC 1 cut(s) 587
Kzo9I GATC 1 cut(s) 292
LweI GCATC 5 cut(s) 94, 198, 702, 1339, 1489
MaeI CTAG 1 cut(s) 1410
MaeIII GTNAC 6 cut(s) 272, 1152, 1285, 1293, 1360, 1466
MalI GATC 1 cut(s) 294
MboI GATC 1 cut(s) 292
MboII GAAGA 6 cut(s) 80, 91, 217, 465, 807, 875
MfeI CAATTG 1 cut(s) 1265
MhlI GDGCHC 2 cut(s) 1274, 1349
MlyI GAGTC 4 cut(s) 31, 484, 796, 889
MmeI TCCRAC 2 cut(s) 159, 490
MnlI CCTC 7 cut(s) 12, 75, 139, 477, 598, 984, 1143
Mph1103I ATGCAT 1 cut(s) 1332
MroI TCCGGA 1 cut(s) 539
MseI TTAA 4 cut(s) 96, 482, 1178, 1223
MspA1I CMGCKG 2 cut(s) 28, 986
MspI CCGG 3 cut(s) 477, 540, 1365
MspR9I CCNGG 2 cut(s) 578, 624
MunI CAATTG 1 cut(s) 1265
Mva1269I GAATGC 1 cut(s) 940
MvaI CCWGG 2 cut(s) 578, 624
NcoI CCATGG 1 cut(s) 570
NdeII GATC 1 cut(s) 292
NlaIII CATG 4 cut(s) 338, 529, 574, 931
NlaIV GGNNCC 3 cut(s) 287, 585, 1272
NmuCI GTSAC 1 cut(s) 1466
NsiI ATGCAT 1 cut(s) 1332
NspI RCATGY 1 cut(s) 529
PaeR7I CTCGAG 1 cut(s) 877
PctI GAATGC 1 cut(s) 940
PfeI GAWTC 3 cut(s) 38, 892, 1093
PflFI GACNNNGTC 1 cut(s) 226
PleI GAGTC 4 cut(s) 30, 484, 796, 888
PpsI GAGTC 4 cut(s) 30, 484, 796, 888
PshAI GACNNNNGTC 1 cut(s) 1464
Psp6I CCWGG 2 cut(s) 576, 622
PspFI CCCAGC 2 cut(s) 687, 819
PspGI CCWGG 2 cut(s) 576, 622
PspN4I GGNNCC 3 cut(s) 287, 585, 1272
PspOMI GGGCCC 1 cut(s) 1270
PspPI GGNCC 4 cut(s) 285, 574, 1270, 1271
PstI CTGCAG 2 cut(s) 33, 166
PsyI GACNNNGTC 1 cut(s) 226
PvuII CAGCTG 2 cut(s) 28, 986
RsaI GTAC 2 cut(s) 585, 1437
RsaNI GTAC 2 cut(s) 584, 1436
SaqAI TTAA 4 cut(s) 96, 482, 1178, 1223
Sau3AI GATC 1 cut(s) 292
Sau96I GGNCC 4 cut(s) 285, 574, 1270, 1271
SchI GAGTC 4 cut(s) 31, 484, 796, 889
ScrFI CCNGG 2 cut(s) 578, 624
SduI GDGCHC 2 cut(s) 1274, 1349
SfaNI GCATC 5 cut(s) 94, 198, 702, 1339, 1489
SfcI CTRYAG 2 cut(s) 29, 162
Sfr274I CTCGAG 1 cut(s) 877
SlaI CTCGAG 1 cut(s) 877
SmlI CTYRAG 2 cut(s) 375, 877
SmoI CTYRAG 2 cut(s) 375, 877
SsiI CCGC 8 cut(s) 422, 459, 659, 1034, 1049, 1052, 1274, 1474
SspI AATATT 1 cut(s) 1026
SspMI CTAG 1 cut(s) 1410
StyD4I CCNGG 2 cut(s) 576, 622
StyI CCWWGG 3 cut(s) 570, 1387, 1455
TaaI ACNGT 1 cut(s) 915
TaqI TCGA 2 cut(s) 800, 878
TatI WGTACW 1 cut(s) 1435
TauI GCSGC 3 cut(s) 661, 1051, 1054
TfiI GAWTC 3 cut(s) 38, 892, 1093
Tru1I TTAA 4 cut(s) 96, 482, 1178, 1223
Tru9I TTAA 4 cut(s) 96, 482, 1178, 1223
TseFI GTSAC 1 cut(s) 1466
Tsp45I GTSAC 1 cut(s) 1466
TspDTI ATGAA 3 cut(s) 432, 546, 980
TspGWI ACGGA 3 cut(s) 114, 205, 795
Tth111I GACNNNGTC 1 cut(s) 226
XapI RAATTY 6 cut(s) 195, 235, 553, 565, 673, 682
XceI RCATGY 1 cut(s) 529
XhoI CTCGAG 1 cut(s) 877
XspI CTAG 1 cut(s) 1410
Zsp2I ATGCAT 1 cut(s) 1332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.