pycom03g03200

DAG protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0028483
Physical Location & Seq
Forward (+)
2531883 .. 2533156
1274 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 618 bp
ATGGTTCAAACCCTAGTCCGAGCAATTGCCGTGGCCGCCGCAGCCGCCACCGCCGCCACCACCGCAACATCTCGCCACCTCACAATCTCTCTCCTCATACCCAAACGCCTCTTCTTGACATCCAAACTTGCCAACCCTCCCTCCATCCCATCCCTCATGCTGGGCCGCCGATCCCTCGCCCCGCCCACCCACGATGTCCGATTCCCTACGGCCACCACCAGGTACACTCCGATCTGTTGCGGGGTCAAATCATGTTCGAAATTTGGCCGTCCACCAGAAGAGATGGAATTGATCCTCCAAGGTTGGGATTACTGGTGGATCGTCATGGAGAAGCCCGGCGTTGAGTGTGATCCAATCGATTACTATGCAGAGACCCTGGTTAAAGTCGTTGGTGTGAGTCTGGAAGTAGCAAAGAAGAAGATATATAAGGTGTATTACGGCTGGAGCTCCGGTTTTTTTTGTAAGATTGATGAGCTGACGGCACGTAAGTTTGAAGGTAGGTTTGTTTGTGGCATTGATTTGCATGTTACTGTTTGGCTTGCCCGGTGTTCTAGGAACTTTCCCGGATCCGGATATGGCCGACGATTGATCCGACCCTTTTTGGCTATCGGCGGATGA

Protein Analysis

206

Amino Acids

22.81

Weight (kDa)

9.57

Isoelectric Point (pI)

39.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MORF_dom PF21864 104 - 165 2.7e-12 Multiple organellar RNA editing factor 1, MORF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0028483)

Species Orthologous Gene IDs
malus_domestica MD03G1041100.v1.1
pyrus_communis pycom03g03200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 569
AclWI GGATC 7 cut(s) 165, 286, 326, 344, 561, 574, 583
AcoI YGGCCR 4 cut(s) 33, 210, 265, 577
AcsI RAATTY 1 cut(s) 260
AfaI GTAC 1 cut(s) 224
AfiI CCNNNNNNNGG 4 cut(s) 160, 219, 304, 569
AgsI TTSAA 2 cut(s) 8, 494
AjnI CCWGG 2 cut(s) 218, 375
AjuI GAANNNNNNNTTGG 2 cut(s) 95, 127
AluBI AGCT 2 cut(s) 447, 475
AluI AGCT 2 cut(s) 447, 475
Alw21I GWGCWC 1 cut(s) 449
Alw26I GTCTC 1 cut(s) 365
AlwI GGATC 7 cut(s) 165, 286, 326, 344, 561, 574, 583
Aor13HI TCCGGA 1 cut(s) 569
AoxI GGCC 5 cut(s) 33, 163, 210, 265, 577
ApeKI GCWGC 1 cut(s) 41
ApoI RAATTY 1 cut(s) 260
AspS9I GGNCC 1 cut(s) 163
AsuC2I CCSGG 3 cut(s) 336, 544, 564
AsuII TTCGAA 1 cut(s) 257
BamHI GGATCC 1 cut(s) 566
BanII GRGCYC 1 cut(s) 449
Bbv12I GWGCWC 1 cut(s) 449
BbvI GCAGC 1 cut(s) 53
BccI CCATC 3 cut(s) 152, 157, 277
BceAI ACGGC 5 cut(s) 14, 225, 252, 454, 495
BcgI CGANNNNNNTGC 2 cut(s) 347, 381
BciT130I CCWGG 2 cut(s) 220, 377
BcnI CCSGG 3 cut(s) 336, 544, 564
BcoDI GTCTC 1 cut(s) 365
BfaI CTAG 2 cut(s) 14, 552
BisI GCNGC 6 cut(s) 36, 39, 42, 45, 54, 166
BlsI GCNGC 6 cut(s) 37, 40, 43, 46, 55, 167
Bme1390I CCNGG 5 cut(s) 220, 336, 377, 544, 564
BmgT120I GGNCC 1 cut(s) 163
BmiI GGNNCC 1 cut(s) 568
BmrFI CCNGG 5 cut(s) 220, 336, 377, 544, 564
BpmI CTGGAG 1 cut(s) 463
Bpu14I TTCGAA 1 cut(s) 257
BpuMI CCSGG 3 cut(s) 336, 544, 564
Bsa29I ATCGAT 1 cut(s) 357
BsaAI YACGTR 1 cut(s) 485
BsaI GGTCTC 1 cut(s) 365
BsaJI CCNNGG 3 cut(s) 30, 298, 375
BsaWI WCCGGW 2 cut(s) 449, 569
BsaXI ACNNNNNCTCC 4 cut(s) 125, 155, 436, 466
Bsc4I CCNNNNNNNGG 4 cut(s) 160, 219, 304, 569
Bse1I ACTGG 1 cut(s) 317
BseAI TCCGGA 1 cut(s) 569
BseBI CCWGG 2 cut(s) 220, 377
BseCI ATCGAT 1 cut(s) 357
BseDI CCNNGG 3 cut(s) 30, 298, 375
BseGI GGATG 3 cut(s) 119, 144, 149
BseLI CCNNNNNNNGG 4 cut(s) 160, 219, 304, 569
BseNI ACTGG 1 cut(s) 317
BseRI GAGGAG 1 cut(s) 83
BseXI GCAGC 1 cut(s) 53
BseYI CCCAGC 1 cut(s) 160
BshFI GGCC 5 cut(s) 35, 165, 212, 267, 579
BshVI ATCGAT 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 449
BsiSI CCGG 5 cut(s) 336, 450, 544, 564, 570
BslI CCNNNNNNNGG 4 cut(s) 160, 219, 304, 569
BsmAI GTCTC 1 cut(s) 365
BsnI GGCC 5 cut(s) 35, 165, 212, 267, 579
Bso31I GGTCTC 1 cut(s) 365
Bsp119I TTCGAA 1 cut(s) 257
Bsp1286I GDGCHC 1 cut(s) 449
Bsp13I TCCGGA 1 cut(s) 569
Bsp143I GATC 7 cut(s) 170, 231, 291, 318, 349, 566, 588
BspANI GGCC 5 cut(s) 35, 165, 212, 267, 579
BspDI ATCGAT 1 cut(s) 357
BspEI TCCGGA 1 cut(s) 569
BspLI GGNNCC 1 cut(s) 568
BspPI GGATC 7 cut(s) 165, 286, 326, 344, 561, 574, 583
BspT104I TTCGAA 1 cut(s) 257
BspTNI GGTCTC 1 cut(s) 365
BsrI ACTGG 1 cut(s) 317
BssECI CCNNGG 3 cut(s) 30, 298, 375
BssMI GATC 7 cut(s) 170, 231, 291, 318, 349, 566, 588
BssT1I CCWWGG 1 cut(s) 298
Bst2UI CCWGG 2 cut(s) 220, 377
Bst4CI ACNGT 1 cut(s) 532
Bst6I CTCTTC 2 cut(s) 116, 273
BstBAI YACGTR 1 cut(s) 485
BstBI TTCGAA 1 cut(s) 257
BstC8I GCNNGC 1 cut(s) 540
BstDSI CCRYGG 1 cut(s) 30
BstF5I GGATG 3 cut(s) 119, 144, 149
BstKTI GATC 7 cut(s) 173, 234, 294, 321, 352, 569, 591
BstMAI GTCTC 1 cut(s) 365
BstMBI GATC 7 cut(s) 170, 231, 291, 318, 349, 566, 588
BstMWI GCNNNNNNNGC 6 cut(s) 35, 41, 44, 50, 53, 62
BstNI CCWGG 2 cut(s) 220, 377
BstNSI RCATGY 1 cut(s) 527
BstSCI CCNGG 5 cut(s) 218, 334, 375, 542, 562
BstV1I GCAGC 1 cut(s) 53
BstX2I RGATCY 1 cut(s) 566
BstYI RGATCY 1 cut(s) 566
Bsu15I ATCGAT 1 cut(s) 357
BsuRI GGCC 5 cut(s) 35, 165, 212, 267, 579
BsuTUI ATCGAT 1 cut(s) 357
BtgI CCRYGG 1 cut(s) 30
BtsCI GGATG 3 cut(s) 119, 144, 149
Cac8I GCNNGC 1 cut(s) 540
Cfr13I GGNCC 1 cut(s) 163
ClaI ATCGAT 1 cut(s) 357
CsiI ACCWGGT 1 cut(s) 218
Csp6I GTAC 1 cut(s) 223
CspCI CAANNNNNGTGG 2 cut(s) 12, 47
CviAII CATG 4 cut(s) 157, 252, 325, 524
CviQI GTAC 1 cut(s) 223
DpnI GATC 7 cut(s) 172, 233, 293, 320, 351, 568, 590
DpnII GATC 7 cut(s) 170, 231, 291, 318, 349, 566, 588
EaeI YGGCCR 4 cut(s) 33, 210, 265, 577
Eam1104I CTCTTC 2 cut(s) 116, 273
EarI CTCTTC 2 cut(s) 116, 273
Ecl136II GAGCTC 1 cut(s) 447
Eco130I CCWWGG 1 cut(s) 298
Eco24I GRGCYC 1 cut(s) 449
Eco31I GGTCTC 1 cut(s) 365
Eco53kI GAGCTC 1 cut(s) 447
EcoICRI GAGCTC 1 cut(s) 447
EcoRII CCWGG 2 cut(s) 218, 375
EcoT14I CCWWGG 1 cut(s) 298
EcoT38I GRGCYC 1 cut(s) 449
ErhI CCWWGG 1 cut(s) 298
FaeI CATG 4 cut(s) 160, 255, 328, 527
FaiI YATR 9 cut(s) 98, 158, 253, 326, 366, 424, 426, 525, 576
FatI CATG 4 cut(s) 156, 251, 324, 523
FauI CCCGC 2 cut(s) 189, 233
Fnu4HI GCNGC 6 cut(s) 36, 39, 42, 45, 54, 166
FokI GGATG 3 cut(s) 106, 131, 136
FriOI GRGCYC 1 cut(s) 449
Fsp4HI GCNGC 6 cut(s) 36, 39, 42, 45, 54, 166
FspBI CTAG 2 cut(s) 14, 552
GluI GCNGC 6 cut(s) 36, 39, 42, 45, 54, 166
GsaI CCCAGC 1 cut(s) 164
GsuI CTGGAG 1 cut(s) 463
HaeIII GGCC 5 cut(s) 35, 165, 212, 267, 579
HapII CCGG 5 cut(s) 336, 450, 544, 564, 570
Hin1II CATG 4 cut(s) 160, 255, 328, 527
HinfI GANTC 2 cut(s) 201, 397
HpaII CCGG 5 cut(s) 336, 450, 544, 564, 570
Hpy166II GTNNAC 2 cut(s) 225, 272
Hpy188I TCNGA 4 cut(s) 20, 200, 231, 593
Hpy188III TCNNGA 3 cut(s) 115, 401, 570
Hpy8I GTNNAC 2 cut(s) 225, 272
Hpy99I CGWCG 1 cut(s) 585
HpyAV CCTTC 1 cut(s) 488
HpyCH4III ACNGT 1 cut(s) 532
HpyCH4IV ACGT 1 cut(s) 484
HpyCH4V TGCA 2 cut(s) 368, 523
HpyF10VI GCNNNNNNNGC 6 cut(s) 35, 41, 44, 50, 53, 62
HpySE526I ACGT 1 cut(s) 484
Hsp92II CATG 4 cut(s) 160, 255, 328, 527
Kpn2I TCCGGA 1 cut(s) 569
Kzo9I GATC 7 cut(s) 170, 231, 291, 318, 349, 566, 588
LmnI GCTCC 2 cut(s) 444, 452
Lsp1109I GCAGC 1 cut(s) 53
MabI ACCWGGT 1 cut(s) 218
MaeI CTAG 2 cut(s) 14, 552
MaeII ACGT 1 cut(s) 484
MaeIII GTNAC 1 cut(s) 526
MalI GATC 7 cut(s) 172, 233, 293, 320, 351, 568, 590
MboI GATC 7 cut(s) 170, 231, 291, 318, 349, 566, 588
MboII GAAGA 4 cut(s) 103, 290, 427, 430
MfeI CAATTG 1 cut(s) 24
MflI RGATCY 1 cut(s) 566
MhlI GDGCHC 1 cut(s) 449
MluCI AATT 3 cut(s) 24, 260, 287
MlyI GAGTC 1 cut(s) 406
MmeI TCCRAC 1 cut(s) 616
MnlI CCTC 8 cut(s) 89, 104, 119, 147, 151, 164, 185, 305
MroI TCCGGA 1 cut(s) 569
MseI TTAA 1 cut(s) 381
MspI CCGG 5 cut(s) 336, 450, 544, 564, 570
MspR9I CCNGG 5 cut(s) 220, 336, 377, 544, 564
MunI CAATTG 1 cut(s) 24
MvaI CCWGG 2 cut(s) 220, 377
MwoI GCNNNNNNNGC 6 cut(s) 35, 41, 44, 50, 53, 62
NciI CCSGG 3 cut(s) 336, 544, 564
NdeII GATC 7 cut(s) 170, 231, 291, 318, 349, 566, 588
NlaIII CATG 4 cut(s) 160, 255, 328, 527
NlaIV GGNNCC 1 cut(s) 568
NspI RCATGY 1 cut(s) 527
NspV TTCGAA 1 cut(s) 257
PcsI WCGNNNNNNNCGW 1 cut(s) 589
PfeI GAWTC 1 cut(s) 201
PfoI TCCNGGA 1 cut(s) 562
PkrI GCNGC 6 cut(s) 37, 40, 43, 46, 55, 167
PleI GAGTC 1 cut(s) 405
PpsI GAGTC 1 cut(s) 405
Ppu21I YACGTR 1 cut(s) 485
Psp124BI GAGCTC 1 cut(s) 449
Psp6I CCWGG 2 cut(s) 218, 375
PspFI CCCAGC 1 cut(s) 160
PspGI CCWGG 2 cut(s) 218, 375
PspN4I GGNNCC 1 cut(s) 568
PspPI GGNCC 1 cut(s) 163
PsuI RGATCY 1 cut(s) 566
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
SacI GAGCTC 1 cut(s) 449
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 6 cut(s) 36, 39, 42, 45, 54, 166
Sau3AI GATC 7 cut(s) 170, 231, 291, 318, 349, 566, 588
Sau96I GGNCC 1 cut(s) 163
SchI GAGTC 1 cut(s) 406
ScrFI CCNGG 5 cut(s) 220, 336, 377, 544, 564
SduI GDGCHC 1 cut(s) 449
SetI ASST 9 cut(s) 81, 224, 304, 432, 449, 477, 487, 499, 503
SexAI ACCWGGT 1 cut(s) 218
SfuI TTCGAA 1 cut(s) 257
Sse9I AATT 3 cut(s) 24, 260, 287
SspMI CTAG 2 cut(s) 14, 552
SstI GAGCTC 1 cut(s) 449
StyD4I CCNGG 5 cut(s) 218, 334, 375, 542, 562
StyI CCWWGG 1 cut(s) 298
TaaI ACNGT 1 cut(s) 532
TaiI ACGT 1 cut(s) 487
TaqI TCGA 2 cut(s) 257, 357
TasI AATT 3 cut(s) 24, 260, 287
TauI GCSGC 5 cut(s) 38, 41, 47, 56, 168
TfiI GAWTC 1 cut(s) 201
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TseI GCWGC 1 cut(s) 41
XapI RAATTY 1 cut(s) 260
XceI RCATGY 1 cut(s) 527
XspI CTAG 2 cut(s) 14, 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.