pycom03g06670

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0028481
Physical Location & Seq
Reverse (-)
5217134 .. 5220006
2873 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1920 bp
ATGACGATGATGATGCAGGTTCATATTGTGTGCATGGGGTCCCTTCCAGATGGGGAGTTCTCACCGGTGTCTCACCACCCAAGTATTCTGCAAAGAGTTGTCCAGGGAAGCCAAAACCTAGTTCTTATCCTCTCTCACTCTCTCAGTTTGCCGGAAAGTTTACTGGTAGTACGAAGTTACAAAAGGAGTTTCAATGGGTTTGCAGCAAAACTCATTGACAAGGAAAGGAAAAAACTTGCTGGTGAGCACTCTGATATAATTTATTATGTCACTACAAAAGACTTTAAAGACGAAGTTTTTGGTCCTCCTCCAAAGAGTTGGAAAGGTGCTTGCGAAGGAGGCAAAAATTTTACTTGCAACAATGTTGCTATAGCCTATGGAAACGGTATTTTGTGCCTATTGGCAGCAACAGGCACGCTTTTGCCTTCATGTCTTCTGACCAGTTTTCGTGTAGTCAAGCTCATCGGAGCTCAATATTACATATCTGAATCTGCAAGGGATGACATCGGTCATGGAACTCACACAGCCTCTATCGCAGCAGGAAACACTGTTAAGGATGTGAGCTTTTATGGACTAGCAAAAGACACTGCAAGAGGAGGTGTTCCTGCAGCAAGAATTGCAACATATAAACTTTGTGATCATGATAAAGGGTGCAGTGCAGATTACATCTTGGCTGCTTTTGACGATGCTATTGATGACGGAGTTAACGTCATTACAATTTCATTTGGATCTGGTTTTGTGACTAGTTTTGATGAGGACCCTTTCACAATTGGTGCTTTTCATGCAATGAAAAAAGGGATACTGACCATACAGCCTGCAGGCAATGAAGGGCCTCAAATCGCTACTGTGTCATGCGTAGCACCATGGATATTGACAGTTGCAGCAAGTACCATAGATCGCCATATCATTGACAAAGTTGTTCTGGCAAATGGAAAAACCCTTGTTGGGAATTCTATTAAGACTTTCACATTATATGGAACAAGTTTTCCCTTAATACGCGGAAAAGATGCTGCAGGAAAGATAGTGGTATGTGATGCTGGATACGGAGATACAACAGCATATGATTCTGGTGCACTTGGTTCAATTTTACATAATGACAACACAGACGATCTTAGTCAAGTTCCCATATTACCTACAACAATGTTAACGATGGAAGAGCATAATGTGGTCAAATCCTACATAAACTCCATCAGGTTGTATTTACATGATTGTGTGTTTCTCTTAATTTATTGTGACCCTCGAACGAAAATACTTAAAAGTGATGCCATAAAAGATCCTTCTGCACCTGCTGTCACTTCCTTCTCCTTGCATGGACCATATCTAATTTTTGCCCCGGGGACTGAACTTTTTGCTGCATATTCACCTAACGCTTCGATCACACAATTGGAAGAAGACAAGAGGCGTGTAAAATACAATATGTTATCTGGAACCTCTGTATCTTGCCCTCATGCTGCTGGTGCAGCTGCATATGTTAAAGCATTCTACCATGATTGGTCTCCCGCCGCCATCAAATCATCTTTTTTGACGACTGGTATATATATTGTCATGATAACAGAAATTCAACAACATTGCCACTGGTGCATTTGCTTATGGATTGGGACACGGCTTGTGTATGAAGCTTGTGAAGAAGATCACATAACGCTGCTATGCATAATGTATGATGAAGGCAAAGTTAGACTCATATCAGGAGATAACAGCACATGCCCTAAAGGATCTGAGAAAGGATATCCAAAGGATCTTAATCACCCTTCAATGGGAGTTAAAGTTGCAGAAAAGGAACCTTTTACAGTTAAATTTCACAGAAGAGTTAAAAATGTAGGCCTTCCAAACCCGACCTACAGGGCCAAAATATTGTCGGATTCCAGAGTTGACAACTTGACATCAAAGTTGTTCCCGAAGTTCTGGCATTCGAGTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

640

Amino Acids

69.61

Weight (kDa)

7.28

Isoelectric Point (pI)

35.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 164 - 510 4.7e-32 Subtilase family
fn3_6 PF17766 579 - 623 8.2e-06 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028481)

Species Orthologous Gene IDs
malus_domestica MD03G1084500.v1.1
pyrus_communis pycom03g06670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1294
Acc36I ACCTGC 2 cut(s) 7, 1294
AccII CGCG 1 cut(s) 999
AciI CCGC 3 cut(s) 999, 1500, 1503
AclWI GGATC 4 cut(s) 736, 1268, 1720, 1743
AcsI RAATTY 4 cut(s) 346, 949, 1557, 1793
AfaI GTAC 2 cut(s) 171, 889
AfiI CCNNNNNNNGG 3 cut(s) 945, 1753, 1754
AgeI ACCGGT 1 cut(s) 64
AgsI TTSAA 4 cut(s) 193, 1083, 1562, 1752
AhlI ACTAGT 1 cut(s) 743
AjnI CCWGG 1 cut(s) 102
AjuI GAANNNNNNNTTGG 2 cut(s) 105, 137
AloI GAACNNNNNNTCC 2 cut(s) 970, 1002
AluBI AGCT 5 cut(s) 460, 470, 564, 1463, 1619
AluI AGCT 5 cut(s) 460, 470, 564, 1463, 1619
Alw21I GWGCWC 3 cut(s) 249, 472, 1075
Alw26I GTCTC 2 cut(s) 75, 1500
Alw44I GTGCAC 1 cut(s) 1071
AlwI GGATC 4 cut(s) 736, 1268, 1720, 1743
Ama87I CYCGRG 1 cut(s) 1333
AoxI GGCC 3 cut(s) 830, 1819, 1842
ApaLI GTGCAC 1 cut(s) 1071
ApoI RAATTY 4 cut(s) 346, 949, 1557, 1793
ArsI GACNNNNNNTTYG 2 cut(s) 281, 313
AsiGI ACCGGT 1 cut(s) 64
AspS9I GGNCC 6 cut(s) 39, 302, 757, 830, 1313, 1842
AsuC2I CCSGG 2 cut(s) 1334, 1335
AsuHPI GGTGA 5 cut(s) 54, 65, 254, 1353, 1736
AvaI CYCGRG 1 cut(s) 1333
AvaII GGWCC 4 cut(s) 39, 302, 757, 1313
BaeGI GKGCMC 1 cut(s) 1075
BaeI ACNNNNGTAYC 2 cut(s) 1419, 1452
BanII GRGCYC 1 cut(s) 472
BbsI GAAGAC 2 cut(s) 425, 1398
Bbv12I GWGCWC 3 cut(s) 249, 472, 1075
BccI CCATC 4 cut(s) 44, 1144, 1196, 1514
BceAI ACGGC 1 cut(s) 1619
BcgI CGANNNNNNTGC 1 cut(s) 29
BciT130I CCWGG 1 cut(s) 104
BciVI GTATCC 2 cut(s) 792, 1034
BclI TGATCA 1 cut(s) 637
BcnI CCSGG 2 cut(s) 1334, 1335
BcoDI GTCTC 2 cut(s) 75, 1500
BcuI ACTAGT 1 cut(s) 743
BfaI CTAG 3 cut(s) 119, 575, 744
BfmI CTRYAG 5 cut(s) 369, 606, 816, 1011, 1837
BfuAI ACCTGC 2 cut(s) 7, 1294
BfuI GTATCC 2 cut(s) 792, 1034
Bme1390I CCNGG 3 cut(s) 104, 1334, 1335
Bme18I GGWCC 4 cut(s) 39, 302, 757, 1313
BmeT110I CYCGRG 1 cut(s) 1333
BmgT120I GGNCC 6 cut(s) 39, 302, 757, 830, 1313, 1842
BmiI GGNNCC 5 cut(s) 40, 41, 759, 1429, 1779
BmrFI CCNGG 3 cut(s) 104, 1334, 1335
BmsI GCATC 5 cut(s) 3, 676, 997, 1024, 1252
BoxI GACNNNNGTC 1 cut(s) 507
BpiI GAAGAC 2 cut(s) 425, 1398
BpuMI CCSGG 2 cut(s) 1334, 1335
BsaBI GATNNNNATC 1 cut(s) 1740
BsaI GGTCTC 1 cut(s) 1500
BsaJI CCNNGG 5 cut(s) 103, 863, 1332, 1333, 1334
BsaWI WCCGGW 1 cut(s) 64
BsaXI ACNNNNNCTCC 6 cut(s) 693, 723, 1169, 1199, 1749, 1779
Bsc4I CCNNNNNNNGG 3 cut(s) 945, 1753, 1754
Bse118I RCCGGY 1 cut(s) 64
Bse1I ACTGG 4 cut(s) 168, 441, 1534, 1580
Bse3DI GCAATG 3 cut(s) 792, 829, 1567
Bse8I GATNNNNATC 1 cut(s) 1740
BseBI CCWGG 1 cut(s) 104
BseDI CCNNGG 5 cut(s) 103, 863, 1332, 1333, 1334
BseGI GGATG 2 cut(s) 505, 562
BseJI GATNNNNATC 1 cut(s) 1740
BseLI CCNNNNNNNGG 3 cut(s) 945, 1753, 1754
BseMI GCAATG 3 cut(s) 792, 829, 1567
BseMII CTCAG 2 cut(s) 157, 1707
BseNI ACTGG 4 cut(s) 168, 441, 1534, 1580
BseRI GAGGAG 2 cut(s) 297, 609
BseSI GKGCMC 1 cut(s) 1075
BsgI GTGCAG 4 cut(s) 673, 678, 1266, 1479
Bsh1236I CGCG 1 cut(s) 999
BshFI GGCC 3 cut(s) 832, 1821, 1844
BshTI ACCGGT 1 cut(s) 64
BsiHKAI GWGCWC 3 cut(s) 249, 472, 1075
BsiHKCI CYCGRG 1 cut(s) 1333
BsiSI CCGG 3 cut(s) 65, 152, 1334
BslFI GGGAC 3 cut(s) 25, 1351, 1612
BslI CCNNNNNNNGG 3 cut(s) 945, 1753, 1754
BsmAI GTCTC 2 cut(s) 75, 1500
BsmFI GGGAC 3 cut(s) 25, 1351, 1612
BsmI GAATGC 2 cut(s) 1478, 1906
BsnI GGCC 3 cut(s) 832, 1821, 1844
Bso31I GGTCTC 1 cut(s) 1500
BsoBI CYCGRG 1 cut(s) 1333
Bsp1286I GDGCHC 3 cut(s) 249, 472, 1075
Bsp143I GATC 9 cut(s) 637, 728, 895, 1108, 1273, 1374, 1630, 1712, 1735
Bsp19I CCATGG 1 cut(s) 863
BspACI CCGC 3 cut(s) 999, 1500, 1503
BspANI GGCC 3 cut(s) 832, 1821, 1844
BspCNI CTCAG 2 cut(s) 156, 1708
BspFNI CGCG 1 cut(s) 999
BspHI TCATGA 2 cut(s) 640, 1545
BspLI GGNNCC 5 cut(s) 40, 41, 759, 1429, 1779
BspMAI CTGCAG 3 cut(s) 610, 820, 1015
BspMI ACCTGC 2 cut(s) 7, 1294
BspPI GGATC 4 cut(s) 736, 1268, 1720, 1743
BspQI GCTCTTC 1 cut(s) 1149
BspTNI GGTCTC 1 cut(s) 1500
BsrDI GCAATG 3 cut(s) 792, 829, 1567
BsrFI RCCGGY 1 cut(s) 64
BsrI ACTGG 4 cut(s) 168, 441, 1534, 1580
BssAI RCCGGY 1 cut(s) 64
BssECI CCNNGG 5 cut(s) 103, 863, 1332, 1333, 1334
BssMI GATC 9 cut(s) 637, 728, 895, 1108, 1273, 1374, 1630, 1712, 1735
BssT1I CCWWGG 1 cut(s) 863
Bst2UI CCWGG 1 cut(s) 104
Bst4CI ACNGT 5 cut(s) 386, 550, 847, 877, 1789
Bst6I CTCTTC 2 cut(s) 1149, 1798
BstAPI GCANNNNNTGC 1 cut(s) 617
BstC8I GCNNGC 4 cut(s) 331, 416, 816, 820
BstDEI CTNAG 3 cut(s) 143, 1112, 1716
BstDSI CCRYGG 1 cut(s) 863
BstF5I GGATG 2 cut(s) 505, 562
BstFNI CGCG 1 cut(s) 999
BstKTI GATC 9 cut(s) 640, 731, 898, 1111, 1276, 1377, 1633, 1715, 1738
BstMAI GTCTC 2 cut(s) 75, 1500
BstMBI GATC 9 cut(s) 637, 728, 895, 1108, 1273, 1374, 1630, 1712, 1735
BstMWI GCNNNNNNNGC 7 cut(s) 339, 533, 617, 782, 1457, 1460, 1578
BstNI CCWGG 1 cut(s) 104
BstNSI RCATGY 1 cut(s) 1704
BstPAI GACNNNNGTC 1 cut(s) 507
BstSCI CCNGG 3 cut(s) 102, 1332, 1333
BstSFI CTRYAG 5 cut(s) 369, 606, 816, 1011, 1837
BstSLI GKGCMC 1 cut(s) 1075
BstUI CGCG 1 cut(s) 999
BstV2I GAAGAC 2 cut(s) 425, 1398
BstX2I RGATCY 4 cut(s) 728, 1273, 1712, 1735
BstXI CCANNNNNNTGG 1 cut(s) 318
BstYI RGATCY 4 cut(s) 728, 1273, 1712, 1735
BsuI GTATCC 2 cut(s) 792, 1034
BsuRI GGCC 3 cut(s) 832, 1821, 1844
BtgI CCRYGG 1 cut(s) 863
BtsCI GGATG 2 cut(s) 505, 562
BtsI GCAGTG 2 cut(s) 585, 661
BtsIMutI CAGTG 4 cut(s) 546, 585, 661, 1573
BveI ACCTGC 2 cut(s) 7, 1294
Cac8I GCNNGC 4 cut(s) 331, 416, 816, 820
CciI TCATGA 2 cut(s) 640, 1545
Cfr10I RCCGGY 1 cut(s) 64
Cfr13I GGNCC 6 cut(s) 39, 302, 757, 830, 1313, 1842
Cfr9I CCCGGG 1 cut(s) 1333
Csp6I GTAC 2 cut(s) 170, 888
CspAI ACCGGT 1 cut(s) 64
CviQI GTAC 2 cut(s) 170, 888
DdeI CTNAG 3 cut(s) 143, 1112, 1716
DpnI GATC 9 cut(s) 639, 730, 897, 1110, 1275, 1376, 1632, 1714, 1737
DpnII GATC 9 cut(s) 637, 728, 895, 1108, 1273, 1374, 1630, 1712, 1735
DraI TTTAAA 1 cut(s) 286
Eam1104I CTCTTC 2 cut(s) 1149, 1798
EarI CTCTTC 2 cut(s) 1149, 1798
Ecl136II GAGCTC 1 cut(s) 470
Eco130I CCWWGG 1 cut(s) 863
Eco147I AGGCCT 1 cut(s) 1821
Eco24I GRGCYC 1 cut(s) 472
Eco31I GGTCTC 1 cut(s) 1500
Eco32I GATATC 1 cut(s) 1727
Eco47I GGWCC 4 cut(s) 39, 302, 757, 1313
Eco53kI GAGCTC 1 cut(s) 470
Eco88I CYCGRG 1 cut(s) 1333
EcoICRI GAGCTC 1 cut(s) 470
EcoO109I RGGNCCY 3 cut(s) 39, 757, 830
EcoRI GAATTC 1 cut(s) 949
EcoRII CCWGG 1 cut(s) 102
EcoRV GATATC 1 cut(s) 1727
EcoT14I CCWWGG 1 cut(s) 863
EcoT22I ATGCAT 1 cut(s) 1652
EcoT38I GRGCYC 1 cut(s) 472
ErhI CCWWGG 1 cut(s) 863
FaqI GGGAC 3 cut(s) 25, 1351, 1612
FauI CCCGC 1 cut(s) 1507
FauNDI CATATG 2 cut(s) 1060, 1468
FbaI TGATCA 1 cut(s) 637
FokI GGATG 2 cut(s) 512, 569
FriOI GRGCYC 1 cut(s) 472
FspBI CTAG 3 cut(s) 119, 575, 744
HaeIII GGCC 3 cut(s) 832, 1821, 1844
HapII CCGG 3 cut(s) 65, 152, 1334
HincII GTYRAC 3 cut(s) 706, 1146, 1870
HindII GTYRAC 3 cut(s) 706, 1146, 1870
HindIII AAGCTT 1 cut(s) 1617
HinfI GANTC 5 cut(s) 488, 1064, 1677, 1859, 1912
HpaI GTTAAC 2 cut(s) 706, 1146
HpaII CCGG 3 cut(s) 65, 152, 1334
HphI GGTGA 5 cut(s) 54, 65, 254, 1353, 1736
Hpy166II GTNNAC 5 cut(s) 161, 706, 1073, 1146, 1870
Hpy188I TCNGA 7 cut(s) 253, 438, 467, 487, 1717, 1858, 1919
Hpy188III TCNNGA 7 cut(s) 47, 641, 1425, 1546, 1686, 1863, 1894
Hpy8I GTNNAC 5 cut(s) 161, 706, 1073, 1146, 1870
HpyAV CCTTC 9 cut(s) 53, 329, 435, 821, 1287, 1309, 1658, 1758, 1832
HpyCH4III ACNGT 5 cut(s) 386, 550, 847, 877, 1789
HpyCH4IV ACGT 1 cut(s) 708
HpyF10VI GCNNNNNNNGC 7 cut(s) 339, 533, 617, 782, 1457, 1460, 1578
HpyF3I CTNAG 3 cut(s) 143, 1112, 1716
HpySE526I ACGT 1 cut(s) 708
KflI GGGWCCC 1 cut(s) 39
Ksp22I TGATCA 1 cut(s) 637
KspAI GTTAAC 2 cut(s) 706, 1146
Kzo9I GATC 9 cut(s) 637, 728, 895, 1108, 1273, 1374, 1630, 1712, 1735
LguI GCTCTTC 1 cut(s) 1149
LmnI GCTCC 1 cut(s) 467
LweI GCATC 5 cut(s) 3, 676, 997, 1024, 1252
MaeI CTAG 3 cut(s) 119, 575, 744
MaeII ACGT 1 cut(s) 708
MaeIII GTNAC 5 cut(s) 176, 268, 739, 1232, 1291
MalI GATC 9 cut(s) 639, 730, 897, 1110, 1275, 1376, 1632, 1714, 1737
MboI GATC 9 cut(s) 637, 728, 895, 1108, 1273, 1374, 1630, 1712, 1735
MboII GAAGA 7 cut(s) 425, 1166, 1400, 1403, 1637, 1640, 1815
MfeI CAATTG 2 cut(s) 768, 1382
MflI RGATCY 4 cut(s) 728, 1273, 1712, 1735
MhlI GDGCHC 3 cut(s) 249, 472, 1075
MlyI GAGTC 1 cut(s) 1671
MmeI TCCRAC 2 cut(s) 299, 1836
Mph1103I ATGCAT 1 cut(s) 1652
MslI CAYNNNNRTG 1 cut(s) 1209
MspA1I CMGCKG 1 cut(s) 1463
MspI CCGG 3 cut(s) 65, 152, 1334
MspR9I CCNGG 3 cut(s) 104, 1334, 1335
MunI CAATTG 2 cut(s) 768, 1382
Mva1269I GAATGC 2 cut(s) 1478, 1906
MvaI CCWGG 1 cut(s) 104
MvnI CGCG 1 cut(s) 999
MwoI GCNNNNNNNGC 7 cut(s) 339, 533, 617, 782, 1457, 1460, 1578
NciI CCSGG 2 cut(s) 1334, 1335
NcoI CCATGG 1 cut(s) 863
NdeI CATATG 2 cut(s) 1060, 1468
NdeII GATC 9 cut(s) 637, 728, 895, 1108, 1273, 1374, 1630, 1712, 1735
NlaIV GGNNCC 5 cut(s) 40, 41, 759, 1429, 1779
NmuCI GTSAC 4 cut(s) 268, 739, 1232, 1291
NsiI ATGCAT 1 cut(s) 1652
NspI RCATGY 1 cut(s) 1704
PagI TCATGA 2 cut(s) 640, 1545
PaqCI CACCTGC 1 cut(s) 1294
PceI AGGCCT 1 cut(s) 1821
PciSI GCTCTTC 1 cut(s) 1149
PcsI WCGNNNNNNNCGW 1 cut(s) 705
PctI GAATGC 2 cut(s) 1478, 1906
PfeI GAWTC 3 cut(s) 488, 1064, 1859
PinAI ACCGGT 1 cut(s) 64
PleI GAGTC 1 cut(s) 1671
PpsI GAGTC 1 cut(s) 1671
PpuMI RGGWCCY 2 cut(s) 39, 757
PshAI GACNNNNGTC 1 cut(s) 507
Psp124BI GAGCTC 1 cut(s) 472
Psp5II RGGWCCY 2 cut(s) 39, 757
Psp6I CCWGG 1 cut(s) 102
PspGI CCWGG 1 cut(s) 102
PspN4I GGNNCC 5 cut(s) 40, 41, 759, 1429, 1779
PspPI GGNCC 6 cut(s) 39, 302, 757, 830, 1313, 1842
PspPPI RGGWCCY 2 cut(s) 39, 757
PstI CTGCAG 3 cut(s) 610, 820, 1015
PsuI RGATCY 4 cut(s) 728, 1273, 1712, 1735
PvuII CAGCTG 1 cut(s) 1463
RsaI GTAC 2 cut(s) 171, 889
RsaNI GTAC 2 cut(s) 170, 888
RseI CAYNNNNRTG 1 cut(s) 1209
SacI GAGCTC 1 cut(s) 472
SapI GCTCTTC 1 cut(s) 1149
Sau3AI GATC 9 cut(s) 637, 728, 895, 1108, 1273, 1374, 1630, 1712, 1735
Sau96I GGNCC 6 cut(s) 39, 302, 757, 830, 1313, 1842
SbfI CCTGCAGG 1 cut(s) 820
SchI GAGTC 1 cut(s) 1671
ScrFI CCNGG 3 cut(s) 104, 1334, 1335
SdaI CCTGCAGG 1 cut(s) 820
SduI GDGCHC 3 cut(s) 249, 472, 1075
SfaNI GCATC 5 cut(s) 3, 676, 997, 1024, 1252
SfcI CTRYAG 5 cut(s) 369, 606, 816, 1011, 1837
SgrAI CRCCGGYG 1 cut(s) 64
SinI GGWCC 4 cut(s) 39, 302, 757, 1313
SmaI CCCGGG 1 cut(s) 1335
SmiMI CAYNNNNRTG 1 cut(s) 1209
SpeI ACTAGT 1 cut(s) 743
Sse8387I CCTGCAGG 1 cut(s) 820
SseBI AGGCCT 1 cut(s) 1821
SsiI CCGC 3 cut(s) 999, 1500, 1503
SspI AATATT 2 cut(s) 476, 1851
SspMI CTAG 3 cut(s) 119, 575, 744
SstI GAGCTC 1 cut(s) 472
StuI AGGCCT 1 cut(s) 1821
StyD4I CCNGG 3 cut(s) 102, 1332, 1333
StyI CCWWGG 1 cut(s) 863
TaaI ACNGT 5 cut(s) 386, 550, 847, 877, 1789
TaiI ACGT 1 cut(s) 711
TaqI TCGA 3 cut(s) 1240, 1373, 1910
TaqII GACCGA 1 cut(s) 497
TauI GCSGC 1 cut(s) 1505
TfiI GAWTC 3 cut(s) 488, 1064, 1859
TscAI CASTG 4 cut(s) 553, 592, 661, 1580
TseFI GTSAC 4 cut(s) 268, 739, 1232, 1291
Tsp45I GTSAC 4 cut(s) 268, 739, 1232, 1291
TspDTI ATGAA 8 cut(s) 11, 417, 711, 770, 803, 840, 1629, 1677
TspGWI ACGGA 2 cut(s) 714, 1059
TspMI CCCGGG 1 cut(s) 1333
TspRI CASTG 4 cut(s) 553, 592, 661, 1580
VneI GTGCAC 1 cut(s) 1071
VpaK11BI GGWCC 4 cut(s) 39, 302, 757, 1313
XapI RAATTY 4 cut(s) 346, 949, 1557, 1793
XceI RCATGY 1 cut(s) 1704
XmaI CCCGGG 1 cut(s) 1333
XspI CTAG 3 cut(s) 119, 575, 744
Zsp2I ATGCAT 1 cut(s) 1652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.