pycom03g19920

Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5- phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0029247
Physical Location & Seq
Reverse (-)
20541658 .. 20542492
835 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 408 bp
ATGGACGCACCAATCTCGCAGGAAGTATTGAACTTTGTTGATATTTTGAGTCCAAATGAAACTGAGCTTGGTCGTTTGACGGGAATGCCGACTGAAACCTTTGACCAGATTAGTAATGCTGTGGTGAAATGCCATAAAATGGGCGTTAAGCAAGTCCTAGTGAAACTTGGGGACAAAGGGTCTGCTCTATTTGTAGAAAGCAAAGAACCACTTAAACAACCTTTCATATCTGCAGCAAAAGTACTTGATACTACTGGAGCCGGCTATACTTTCACTGCCTCTTTCGCGGTGGCTCGTGGATGGCAAGTCCAGAAGGGAATGCTTGAGATTTGCAGCTGCGGCGGCTTCTCTTTGTGTGCAAGTAAAGGGGACCCATTCCTAGCATGCCGGAGAGAAAATCCGTTTTGA

Protein Analysis

136

Amino Acids

14.56

Weight (kDa)

6.56

Isoelectric Point (pI)

46.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phos_pyr_kin PF08543 5 - 96 1.2e-07 Phosphomethylpyrimidine kinase
PfkB PF00294 7 - 98 1.1e-17 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0029247)

Species Orthologous Gene IDs
pyrus_communis pycom03g19920
rosa_multiflora Rmu_sc0009042.1_g000006

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 139
AccII CGCG 1 cut(s) 287
AciI CCGC 3 cut(s) 287, 339, 342
AfaI GTAC 1 cut(s) 243
AfiI CCNNNNNNNGG 1 cut(s) 139
AgsI TTSAA 1 cut(s) 31
AhdI GACNNNNNGTC 1 cut(s) 178
AjuI GAANNNNNNNTTGG 2 cut(s) 51, 83
AluBI AGCT 2 cut(s) 67, 336
AluI AGCT 2 cut(s) 67, 336
ApeKI GCWGC 3 cut(s) 233, 333, 336
AspS9I GGNCC 1 cut(s) 370
AsuHPI GGTGA 1 cut(s) 136
AvaII GGWCC 1 cut(s) 370
BauI CACGAG 1 cut(s) 294
BbvI GCAGC 3 cut(s) 245, 323, 345
BccI CCATC 1 cut(s) 294
BcgI CGANNNNNNTGC 1 cut(s) 31
BfaI CTAG 2 cut(s) 158, 380
BfmI CTRYAG 1 cut(s) 231
BisI GCNGC 5 cut(s) 234, 334, 337, 340, 343
BlsI GCNGC 5 cut(s) 235, 335, 338, 341, 344
BmcAI AGTACT 1 cut(s) 243
Bme18I GGWCC 1 cut(s) 370
BmeRI GACNNNNNGTC 1 cut(s) 178
BmgT120I GGNCC 1 cut(s) 370
BmiI GGNNCC 3 cut(s) 259, 371, 372
BpmI CTGGAG 1 cut(s) 276
BpuEI CTTGAG 1 cut(s) 344
Bsc4I CCNNNNNNNGG 1 cut(s) 139
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 1 cut(s) 259
BseGI GGATG 1 cut(s) 305
BseLI CCNNNNNNNGG 1 cut(s) 139
BseMII CTCAG 1 cut(s) 54
BseNI ACTGG 1 cut(s) 259
BseXI GCAGC 3 cut(s) 245, 323, 345
Bsh1236I CGCG 1 cut(s) 287
BsiSI CCGG 2 cut(s) 261, 388
BslFI GGGAC 2 cut(s) 185, 383
BslI CCNNNNNNNGG 1 cut(s) 139
BsmFI GGGAC 2 cut(s) 185, 383
BsmI GAATGC 2 cut(s) 90, 324
BspACI CCGC 3 cut(s) 287, 339, 342
BspCNI CTCAG 1 cut(s) 55
BspFNI CGCG 1 cut(s) 287
BspLI GGNNCC 3 cut(s) 259, 371, 372
BspMAI CTGCAG 1 cut(s) 235
BsrFI RCCGGY 1 cut(s) 260
BsrI ACTGG 1 cut(s) 259
BssAI RCCGGY 1 cut(s) 260
BssSI CACGAG 1 cut(s) 294
Bst2BI CACGAG 1 cut(s) 294
BstC8I GCNNGC 2 cut(s) 262, 385
BstDEI CTNAG 1 cut(s) 63
BstF5I GGATG 1 cut(s) 305
BstFNI CGCG 1 cut(s) 287
BstMWI GCNNNNNNNGC 3 cut(s) 284, 339, 342
BstNSI RCATGY 1 cut(s) 387
BstSFI CTRYAG 1 cut(s) 231
BstUI CGCG 1 cut(s) 287
BstV1I GCAGC 3 cut(s) 245, 323, 345
BtsCI GGATG 1 cut(s) 305
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 1 cut(s) 273
Cac8I GCNNGC 2 cut(s) 262, 385
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 1 cut(s) 370
CseI GACGC 1 cut(s) 14
Csp6I GTAC 1 cut(s) 242
CviAII CATG 1 cut(s) 384
CviJI RGCY 6 cut(s) 67, 260, 264, 293, 336, 345
CviKI_1 RGCY 6 cut(s) 67, 260, 264, 293, 336, 345
CviQI GTAC 1 cut(s) 242
DdeI CTNAG 1 cut(s) 63
DriI GACNNNNNGTC 1 cut(s) 178
Eam1105I GACNNNNNGTC 1 cut(s) 178
Eco47I GGWCC 1 cut(s) 370
EcoO109I RGGNCCY 1 cut(s) 370
FaeI CATG 1 cut(s) 387
FaiI YATR 4 cut(s) 135, 227, 267, 385
FalI AAGNNNNNCTT 2 cut(s) 195, 227
FaqI GGGAC 2 cut(s) 185, 383
FatI CATG 1 cut(s) 383
Fnu4HI GCNGC 5 cut(s) 234, 334, 337, 340, 343
FokI GGATG 1 cut(s) 312
Fsp4HI GCNGC 5 cut(s) 234, 334, 337, 340, 343
FspBI CTAG 2 cut(s) 158, 380
GluI GCNGC 5 cut(s) 234, 334, 337, 340, 343
GsuI CTGGAG 1 cut(s) 276
HapII CCGG 2 cut(s) 261, 388
HgaI GACGC 1 cut(s) 14
Hin1II CATG 1 cut(s) 387
HinfI GANTC 1 cut(s) 49
HpaII CCGG 2 cut(s) 261, 388
HphI GGTGA 1 cut(s) 136
Hpy188III TCNNGA 1 cut(s) 310
HpyAV CCTTC 1 cut(s) 307
HpyCH4V TGCA 3 cut(s) 233, 333, 359
HpyF10VI GCNNNNNNNGC 3 cut(s) 284, 339, 342
HpyF3I CTNAG 1 cut(s) 63
Hsp92II CATG 1 cut(s) 387
KflI GGGWCCC 1 cut(s) 370
KroI GCCGGC 1 cut(s) 260
KroNI GCCGGC 1 cut(s) 262
LmnI GCTCC 1 cut(s) 257
LpnPI CCDG 6 cut(s) 5, 119, 240, 274, 323, 401
Lsp1109I GCAGC 3 cut(s) 245, 323, 345
MaeI CTAG 2 cut(s) 158, 380
MlyI GAGTC 1 cut(s) 58
MnlI CCTC 1 cut(s) 289
MroNI GCCGGC 1 cut(s) 260
MseI TTAA 2 cut(s) 147, 213
MspA1I CMGCKG 1 cut(s) 336
MspI CCGG 2 cut(s) 261, 388
Mva1269I GAATGC 2 cut(s) 90, 324
MvnI CGCG 1 cut(s) 287
MwoI GCNNNNNNNGC 3 cut(s) 284, 339, 342
NaeI GCCGGC 1 cut(s) 262
NgoMIV GCCGGC 1 cut(s) 260
NlaIII CATG 1 cut(s) 387
NlaIV GGNNCC 3 cut(s) 259, 371, 372
NspI RCATGY 1 cut(s) 387
PaeI GCATGC 1 cut(s) 387
PcsI WCGNNNNNNNCGW 1 cut(s) 86
PctI GAATGC 2 cut(s) 90, 324
PdiI GCCGGC 1 cut(s) 262
PflMI CCANNNNNTGG 1 cut(s) 139
PkrI GCNGC 5 cut(s) 235, 335, 338, 341, 344
PleI GAGTC 1 cut(s) 57
PpsI GAGTC 1 cut(s) 57
PpuMI RGGWCCY 1 cut(s) 370
Psp5II RGGWCCY 1 cut(s) 370
PspN4I GGNNCC 3 cut(s) 259, 371, 372
PspPI GGNCC 1 cut(s) 370
PspPPI RGGWCCY 1 cut(s) 370
PstI CTGCAG 1 cut(s) 235
PvuII CAGCTG 1 cut(s) 336
RsaI GTAC 1 cut(s) 243
RsaNI GTAC 1 cut(s) 242
SaqAI TTAA 2 cut(s) 147, 213
SatI GCNGC 5 cut(s) 234, 334, 337, 340, 343
Sau96I GGNCC 1 cut(s) 370
ScaI AGTACT 1 cut(s) 243
SchI GAGTC 1 cut(s) 58
SetI ASST 4 cut(s) 69, 101, 223, 338
SfcI CTRYAG 1 cut(s) 231
SinI GGWCC 1 cut(s) 370
SmlI CTYRAG 1 cut(s) 323
SmoI CTYRAG 1 cut(s) 323
SphI GCATGC 1 cut(s) 387
SsiI CCGC 3 cut(s) 287, 339, 342
SspMI CTAG 2 cut(s) 158, 380
TatI WGTACW 1 cut(s) 241
TauI GCSGC 2 cut(s) 342, 345
Tru1I TTAA 2 cut(s) 147, 213
Tru9I TTAA 2 cut(s) 147, 213
TscAI CASTG 1 cut(s) 280
TseI GCWGC 3 cut(s) 233, 333, 336
TspDTI ATGAA 2 cut(s) 72, 214
TspGWI ACGGA 1 cut(s) 390
TspRI CASTG 1 cut(s) 280
Van91I CCANNNNNTGG 1 cut(s) 139
VpaK11BI GGWCC 1 cut(s) 370
XceI RCATGY 1 cut(s) 387
XspI CTAG 2 cut(s) 158, 380
ZrmI AGTACT 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.