Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr3

Orthogroup ID

OG0028502

Physical Location & Seq

Genomic Coordinates Reverse (-)

23151640 .. 23155797

Gene Structure 4158 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2688 bp

ATGAACAAGATTGAGAGGGAGAGTAAAGCCCAAAATGCTCCAGTTTTGATGAACAAGACGGAAGAGCAAAATGCAGCTGCATTGAGTGAAAAAATAGTTCAAGCTAAACGTAGAAAGAAGAAGAAGGCCAGAGCAACTTCATTTGATCAGATGACTCAAATTTCAAGTAGCGGACCGCTGTTGGTCGAAGAAGACAAGAAAATAGCTAAATTAGGAGGATGTGATTTTAGTACAAGAGGGAGGTGGGTCGAAGACGAGAGCTATCCGTTGTACACAAGTCGTTCATGTCCTTTCATAGAAGAAGGTTTTAGTTGTAATGGGAGATTAGACAAAAACTTCACTAAGTGGAGATGGCAACCACAAGATTGTGACATTCCAAGGTTCGACGCAAGAAAAATGCTTGAATTTATCAGAGGGAAGAGGCTAGTGTTTGTGGGCGATTCAATCAATAAGAATCAATGGCAATCTATGGTGTGCATGCTTGTCGGAGATAAAAGAAATCCAAATAAGCCCAGATTCATGAGAGGGAGAGGAGGCTATAGTGTCGTCTTTGTGGATTATAAGTGTAAAGTTGAATTCTATCCGTCCGTTTTCTTGGTTGAAAAAGTCATGACAAGAGTAGACCAGAAGCAGGAGCAAACGTCCTTGCGAATTGATTCCATTGATCACGGTTCACATCGATGGAGAGAAGCTGATATTTTGGTATTCAACACCGAACATTGGTGGGTCGATTTCAAAACAAATGCCGGGGTTAACTTCTTTCAGGAAGGTGACAAACTTCATCCATATCTTGATGTTCTCACAGCTCTCAGAAAAGGTTTGATGACGTGGGCATCATGGGTTGATAATTACATCAATCCACGCAAAACTCAAGTTTTCTTTCGAACTTCATCGCCAACGCACTTCAGCGGAGGAAAATGGAATTTACGGCAGAGTTGTGTAGGAGTTAGTGAGCCCCTCCTTCAACCTTCAGGAATTGTAAACGAGAAAGATGTAATTTTGGAGAAGGTCCTAAATCAGATGAAGACTCGAGTCACTTTGTTGAACACAACAAGCTTGTCGGAGTATCGAATGGATGCTCATCCATCCGACATCAGAGATTGCAGCCATTGGTGCCTTCCTGGCATTCCTGATGTATGGAATGAATTGTTATATCTTCATTTGAGGGTATCCCAAGCAGTGGGCGCACAAAATGATTTACTTGTCAAAAATGAAGACGGAGGCGCCAGCGAGTCAGACGTTTTTACTAACGTCACGGTAGTCCCGGCTCAAAGTGCACCAGCTGCAACCTTATTTGACAAGATTGAGAACCAAAATGCTCCGTTGGTGAACACGACAAAAGAGCAAAATGAACCGATGTCATTGATGAACAAGACCGATCAAGAGCAAAGTGCGCCAATATCGGTGAACAGGACTGATCAAGAGCAAAATGCTGCATTGATGAACAAGATTGAGAGGACGAGGGAAGCCCAAAATGCTCCAATATTGATGAATAAGGCTGATCAAGAGCAAAACCCTCCAACATTGATGAACAAGACTACTGACGAGCAAAACGCTGCATTGGTTGAAAAACCAATAAAAAAGAGGATGGCGGGAAAGAGGAAGAAGAAGAAGAGCATAGCAACATCATTTGATCAAAAGAGTAGTACTACTCAAATTTCAAGTAGCGGCGGGATGCAAGACATTGAAGAAAACAAGAAGAGAACAACAAAATTAGGAGGATGTGATTTTAGTACAAGAGGGAGGTGGGTCTATGATGAAAGCTATCCGTTGTACACACGTCAATCATGTCCTTTCATATATGAAGGTTTTGATTGTCAAGGCAGTGGGAGATTAGATAAAAACTTCACCAAGTGGAGGTGGCAACCACAAGATTGTGAAATTCCAAGGTTCGATGCAACAAAAATGCTGGAACTGATGAGAGGTAAACGGCTACTTTATGTGGGGGATTCAATCAATAGAAATCAATTTGAATCCATAGTGTGCATGCTAATGGGAGATATAAGAGACCCCAATAGGCCTAGAATCTCCCATGCCAAGGGGACTTATAGTTTTTACTTTGTGGATTACAAGTTTACAGTCGAGTTTTCTCCATCCGTTTTCTTGGTTGAGAAAGGCACAAAAAGAGTAGGGCAGAAGCAGGTGCAAACGTCCTTGCGAATTGATACCATTGATCAGGGTTCATCTAGATGGAGAGGAGCTGATATTTTGGTATTCGACACAGAACATTGGTGGGCCGATTCCAAAACTAACTCTGGGAAGAATTTTTACCAGGAAGGGGGAAAAATTCATCCATATCTTGATGTTCACACAGCTCTCAGAAAAGGTTTGATGACCTGGGCAGCATGGGTTGATAAAAGTATCAATCCACGCAAAACCCAAGTTTTCTTTCGAACTTCATCACCAACGCATTTCAGCGGAGGTCAGTGGAAATCGAGCCGGAGTTGTATAGGAGCTACTGAACCCCTCCCCAAACCTTCAGGAATGGCAAACAAGAAAGATGTAGTCCTAGAGGAGGTTGTAAAGCAGATGAAAACTCGTGTGACATTGTTGAACATAACAAGCCTGTCAGAATATAGAATAGATGCTCATCCATCAGCCATCAAAGATTGCAGCCATTGGTGTCTTCCTGGCCTGCCTGATGCCTGGAATGAATTGTTGTATCTGCACTTCCTCAGTGACTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0008150 GO:0008374 GO:0009987 GO:0012505 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071554

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

896

Amino Acids

102.49

Weight (kDa)

9.13

Isoelectric Point (pI)

46.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	74 - 124	2.7e-17	PMR5 N terminal Domain
PC-Esterase	PF13839	125 - 385	2.4e-82	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
PMR5N	PF14416	575 - 627	2.4e-19	PMR5 N terminal Domain
PC-Esterase	PF13839	628 - 888	4.4e-86	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0028502)

Species	Orthologous Gene IDs
malus_domestica	MD03G1294300.v1.1
pyrus_communis	pycom03g23260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	561
AarI	CACCTGC	1 cut(s)	2131
Acc36I	ACCTGC	1 cut(s)	2131
AccB1I	GGYRCC	2 cut(s)	1113, 1223
AccB7I	CCANNNNNTGG	1 cut(s)	1180
AccI	GTMKAC	1 cut(s)	621
AciI	CCGC	7 cut(s)	171, 176, 909, 1592, 1668, 1671, 2418
AcsI	RAATTY	8 cut(s)	159, 404, 575, 922, 1656, 1881, 2263, 2286
AcuI	CTGAAG	3 cut(s)	889, 954, 2463
AcyI	GRCGYC	1 cut(s)	1224
AdeI	CACNNNGTG	2 cut(s)	345, 1854
AfaI	GTAC	5 cut(s)	232, 272, 1648, 1735, 1775
AfiI	CCNNNNNNNGG	7 cut(s)	631, 720, 1180, 1857, 2038, 2278, 2515
AflIII	ACRYGT	1 cut(s)	1778
AjiI	CACGTC	2 cut(s)	828, 1781
AjnI	CCWGG	5 cut(s)	1120, 2271, 2336, 2629, 2645
AjuI	GAANNNNNNNTTGG	2 cut(s)	2373, 2405
Alw21I	GWGCWC	1 cut(s)	1279
Alw26I	GTCTC	1 cut(s)	2001
Alw44I	GTGCAC	1 cut(s)	1275
Ama87I	CYCGRG	1 cut(s)	1029
AoxI	GGCC	4 cut(s)	126, 2018, 2235, 2632
ApaLI	GTGCAC	1 cut(s)	1275
ApeKI	GCWGC	8 cut(s)	74, 77, 1104, 1283, 1433, 1556, 2342, 2613
ApoI	RAATTY	8 cut(s)	159, 404, 575, 922, 1656, 1881, 2263, 2286
Asp700I	GAANNNNTTC	1 cut(s)	655
AspLEI	GCGC	3 cut(s)	1187, 1226, 1396
AspS9I	GGNCC	3 cut(s)	173, 1009, 2235
AsuC2I	CCSGG	2 cut(s)	748, 1265
AsuHPI	GGTGA	5 cut(s)	782, 1339, 1417, 1840, 2394
AsuII	TTCGAA	2 cut(s)	883, 2392
AvaI	CYCGRG	1 cut(s)	1029
AvaII	GGWCC	2 cut(s)	173, 1009
BaeGI	GKGCMC	1 cut(s)	1279
BanI	GGYRCC	2 cut(s)	1113, 1223
BanII	GRGCYC	1 cut(s)	957
BarI	GAAGNNNNNNTAC	4 cut(s)	2252, 2284, 2654, 2686
BauI	CACGAG	2 cut(s)	2538, 2683
BbsI	GAAGAC	5 cut(s)	198, 258, 1031, 1221, 2618
Bbv12I	GWGCWC	1 cut(s)	1279
BbvI	GCAGC	8 cut(s)	64, 86, 1116, 1270, 1420, 1543, 2354, 2625
BccI	CCATC	8 cut(s)	345, 675, 1093, 1582, 2101, 2184, 2602, 2609
BceAI	ACGGC	2 cut(s)	944, 1946
BcgI	CGANNNNNNTGC	2 cut(s)	466, 500
BciT130I	CCWGG	5 cut(s)	1122, 2273, 2338, 2631, 2647
BciVI	GTATCC	1 cut(s)	1180
BclI	TGATCA	6 cut(s)	145, 664, 1417, 1501, 1633, 2173
BcnI	CCSGG	2 cut(s)	748, 1265
BcoDI	GTCTC	1 cut(s)	2001
BfaI	CTAG	4 cut(s)	425, 2022, 2187, 2510
BfmI	CTRYAG	1 cut(s)	538
BfoI	RGCGCY	1 cut(s)	1227
BfuAI	ACCTGC	1 cut(s)	2131
BfuI	GTATCC	1 cut(s)	1180
BglI	GCCNNNNNGGC	1 cut(s)	1122
BisI	GCNGC	9 cut(s)	75, 78, 1105, 1284, 1434, 1557, 1669, 2343, 2614
BlsI	GCNGC	9 cut(s)	76, 79, 1106, 1285, 1435, 1558, 1670, 2344, 2615
BmcAI	AGTACT	1 cut(s)	1648
Bme1390I	CCNGG	7 cut(s)	748, 1122, 1265, 2273, 2338, 2631, 2647
Bme18I	GGWCC	2 cut(s)	173, 1009
BmeT110I	CYCGRG	1 cut(s)	1029
BmgBI	CACGTC	2 cut(s)	828, 1781
BmgT120I	GGNCC	3 cut(s)	173, 1009, 2235
BmiI	GGNNCC	2 cut(s)	1115, 1225
BmrFI	CCNGG	7 cut(s)	748, 1122, 1265, 2273, 2338, 2631, 2647
BmsI	GCATC	6 cut(s)	842, 1066, 1665, 1885, 2575, 2632
BoxI	GACNNNNGTC	1 cut(s)	1031
BpiI	GAAGAC	5 cut(s)	198, 258, 1031, 1221, 2618
BpmI	CTGGAG	1 cut(s)	24
Bpu14I	TTCGAA	2 cut(s)	883, 2392
BpuEI	CTTGAG	1 cut(s)	855
BpuMI	CCSGG	2 cut(s)	748, 1265
Bsa29I	ATCGAT	1 cut(s)	679
BsaBI	GATNNNNATC	2 cut(s)	1080, 2589
BsaHI	GRCGYC	1 cut(s)	1224
BsaI	GGTCTC	1 cut(s)	2001
BsaJI	CCNNGG	5 cut(s)	377, 747, 1886, 2037, 2337
BsaXI	ACNNNNNCTCC	2 cut(s)	626, 656
Bsc4I	CCNNNNNNNGG	7 cut(s)	631, 720, 1180, 1857, 2038, 2278, 2515
Bse1I	ACTGG	1 cut(s)	41
Bse8I	GATNNNNATC	2 cut(s)	1080, 2589
BseBI	CCWGG	5 cut(s)	1122, 2273, 2338, 2631, 2647
BseCI	ATCGAT	1 cut(s)	679
BseDI	CCNNGG	5 cut(s)	377, 747, 1886, 2037, 2337
BseJI	GATNNNNATC	2 cut(s)	1080, 2589
BseLI	CCNNNNNNNGG	7 cut(s)	631, 720, 1180, 1857, 2038, 2278, 2515
BseMII	CTCAG	2 cut(s)	823, 2332
BseNI	ACTGG	1 cut(s)	41
BseRI	GAGGAG	3 cut(s)	546, 2211, 2528
BseSI	GKGCMC	1 cut(s)	1279
BseXI	GCAGC	8 cut(s)	64, 86, 1116, 1270, 1420, 1543, 2354, 2625
BsgI	GTGCAG	1 cut(s)	2651
BshFI	GGCC	4 cut(s)	128, 2020, 2237, 2634
BshNI	GGYRCC	2 cut(s)	1113, 1223
BshVI	ATCGAT	1 cut(s)	679
BsiHKAI	GWGCWC	1 cut(s)	1279
BsiHKCI	CYCGRG	1 cut(s)	1029
BsiSI	CCGG	3 cut(s)	747, 1265, 2440
BslFI	GGGAC	2 cut(s)	1247, 2056
BslI	CCNNNNNNNGG	7 cut(s)	631, 720, 1180, 1857, 2038, 2278, 2515
BsmAI	GTCTC	1 cut(s)	2001
BsmFI	GGGAC	2 cut(s)	1247, 2056
BsmI	GAATGC	1 cut(s)	1125
BsnI	GGCC	4 cut(s)	128, 2020, 2237, 2634
Bso31I	GGTCTC	1 cut(s)	2001
BsoBI	CYCGRG	1 cut(s)	1029
Bsp119I	TTCGAA	2 cut(s)	883, 2392
Bsp1286I	GDGCHC	2 cut(s)	957, 1279
Bsp1407I	TGTACA	2 cut(s)	270, 1773
Bsp143I	GATC	7 cut(s)	145, 664, 1378, 1417, 1501, 1633, 2173
BspACI	CCGC	7 cut(s)	171, 176, 909, 1592, 1668, 1671, 2418
BspANI	GGCC	4 cut(s)	128, 2020, 2237, 2634
BspCNI	CTCAG	3 cut(s)	822, 2331, 2688
BspDI	ATCGAT	1 cut(s)	679
BspHI	TCATGA	2 cut(s)	519, 609
BspLI	GGNNCC	2 cut(s)	1115, 1225
BspMI	ACCTGC	1 cut(s)	2131
BspQI	GCTCTTC	2 cut(s)	57, 1607
BspT104I	TTCGAA	2 cut(s)	883, 2392
BspT107I	GGYRCC	2 cut(s)	1113, 1223
BspTNI	GGTCTC	1 cut(s)	2001
BsrGI	TGTACA	2 cut(s)	270, 1773
BsrI	ACTGG	1 cut(s)	41
BssECI	CCNNGG	5 cut(s)	377, 747, 1886, 2037, 2337
BssMI	GATC	7 cut(s)	145, 664, 1378, 1417, 1501, 1633, 2173
BssNI	GRCGYC	1 cut(s)	1224
BssSI	CACGAG	2 cut(s)	2538, 2683
BssT1I	CCWWGG	3 cut(s)	377, 1886, 2037
Bst2BI	CACGAG	2 cut(s)	2538, 2683
Bst2UI	CCWGG	5 cut(s)	1122, 2273, 2338, 2631, 2647
Bst4CI	ACNGT	3 cut(s)	671, 1258, 2080
Bst6I	CTCTTC	4 cut(s)	57, 413, 1607, 1694
BstACI	GRCGYC	1 cut(s)	1224
BstAPI	GCANNNNNTGC	1 cut(s)	1283
BstAUI	TGTACA	2 cut(s)	270, 1773
BstBI	TTCGAA	2 cut(s)	883, 2392
BstC8I	GCNNGC	4 cut(s)	479, 1228, 1988, 2636
BstDEI	CTNAG	4 cut(s)	342, 809, 2318, 2675
BstENI	CCTNNNNNAGG	1 cut(s)	2513
BstH2I	RGCGCY	1 cut(s)	1227
BstHHI	GCGC	3 cut(s)	1187, 1226, 1396
BstKTI	GATC	7 cut(s)	148, 667, 1381, 1420, 1504, 1636, 2176
BstMAI	GTCTC	1 cut(s)	2001
BstMBI	GATC	7 cut(s)	145, 664, 1378, 1417, 1501, 1633, 2173
BstMWI	GCNNNNNNNGC	8 cut(s)	35, 1113, 1122, 1184, 1274, 1283, 1393, 1475
BstNI	CCWGG	5 cut(s)	1122, 2273, 2338, 2631, 2647
BstNSI	RCATGY	2 cut(s)	481, 1990
BstPAI	GACNNNNGTC	1 cut(s)	1031
BstSCI	CCNGG	7 cut(s)	746, 1120, 1263, 2271, 2336, 2629, 2645
BstSFI	CTRYAG	1 cut(s)	538
BstSLI	GKGCMC	1 cut(s)	1279
BstV1I	GCAGC	8 cut(s)	64, 86, 1116, 1270, 1420, 1543, 2354, 2625
BstV2I	GAAGAC	5 cut(s)	198, 258, 1031, 1221, 2618
Bsu15I	ATCGAT	1 cut(s)	679
BsuI	GTATCC	1 cut(s)	1180
BsuRI	GGCC	4 cut(s)	128, 2020, 2237, 2634
BsuTUI	ATCGAT	1 cut(s)	679
BtgZI	GCGATG	1 cut(s)	876
BtrI	CACGTC	2 cut(s)	828, 1781
BtsI	GCAGTG	2 cut(s)	1185, 1831
BtsIMutI	CAGTG	4 cut(s)	1185, 1831, 2432, 2683
BveI	ACCTGC	1 cut(s)	2131
Cac8I	GCNNGC	4 cut(s)	479, 1228, 1988, 2636
CciI	TCATGA	2 cut(s)	519, 609
CfoI	GCGC	3 cut(s)	1187, 1226, 1396
Cfr13I	GGNCC	3 cut(s)	173, 1009, 2235
ClaI	ATCGAT	1 cut(s)	679
CpoI	CGGWCCG	1 cut(s)	173
CseI	GACGC	1 cut(s)	395
Csp6I	GTAC	5 cut(s)	231, 271, 1647, 1734, 1774
CspI	CGGWCCG	1 cut(s)	173
CviAII	CATG	9 cut(s)	285, 478, 520, 610, 837, 1788, 1987, 2033, 2346
CviQI	GTAC	5 cut(s)	231, 271, 1647, 1734, 1774
DdeI	CTNAG	4 cut(s)	342, 809, 2318, 2675
DinI	GGCGCC	1 cut(s)	1225
DpnI	GATC	7 cut(s)	147, 666, 1380, 1419, 1503, 1635, 2175
DpnII	GATC	7 cut(s)	145, 664, 1378, 1417, 1501, 1633, 2173
DraIII	CACNNNGTG	2 cut(s)	345, 1854
Eam1104I	CTCTTC	4 cut(s)	57, 413, 1607, 1694
EarI	CTCTTC	4 cut(s)	57, 413, 1607, 1694
Eco130I	CCWWGG	3 cut(s)	377, 1886, 2037
Eco147I	AGGCCT	1 cut(s)	2020
Eco24I	GRGCYC	1 cut(s)	957
Eco31I	GGTCTC	1 cut(s)	2001
Eco47I	GGWCC	2 cut(s)	173, 1009
Eco57I	CTGAAG	3 cut(s)	889, 954, 2463
Eco88I	CYCGRG	1 cut(s)	1029
EcoNI	CCTNNNNNAGG	1 cut(s)	2513
EcoO109I	RGGNCCY	1 cut(s)	1009
EcoRI	GAATTC	1 cut(s)	575
EcoRII	CCWGG	5 cut(s)	1120, 2271, 2336, 2629, 2645
EcoT14I	CCWWGG	3 cut(s)	377, 1886, 2037
EcoT38I	GRGCYC	1 cut(s)	957
EgeI	GGCGCC	1 cut(s)	1225
EheI	GGCGCC	1 cut(s)	1225
ErhI	CCWWGG	3 cut(s)	377, 1886, 2037
FaeI	CATG	9 cut(s)	288, 481, 523, 613, 840, 1791, 1990, 2036, 2349
FaqI	GGGAC	2 cut(s)	1247, 2056
FatI	CATG	9 cut(s)	284, 477, 519, 609, 836, 1787, 1986, 2032, 2345
FauI	CCCGC	2 cut(s)	1585, 1664
FbaI	TGATCA	6 cut(s)	145, 664, 1417, 1501, 1633, 2173
FblI	GTMKAC	1 cut(s)	621
Fnu4HI	GCNGC	9 cut(s)	75, 78, 1105, 1284, 1434, 1557, 1669, 2343, 2614
FriOI	GRGCYC	1 cut(s)	957
Fsp4HI	GCNGC	9 cut(s)	75, 78, 1105, 1284, 1434, 1557, 1669, 2343, 2614
FspBI	CTAG	4 cut(s)	425, 2022, 2187, 2510
GlaI	GCGC	3 cut(s)	1186, 1225, 1395
GluI	GCNGC	9 cut(s)	75, 78, 1105, 1284, 1434, 1557, 1669, 2343, 2614
GsuI	CTGGAG	1 cut(s)	24
HaeII	RGCGCY	1 cut(s)	1227
HaeIII	GGCC	4 cut(s)	128, 2020, 2237, 2634
HapII	CCGG	3 cut(s)	747, 1265, 2440
HgaI	GACGC	1 cut(s)	395
HhaI	GCGC	3 cut(s)	1187, 1226, 1396
Hin1I	GRCGYC	1 cut(s)	1224
Hin1II	CATG	9 cut(s)	288, 481, 523, 613, 840, 1791, 1990, 2036, 2349
Hin6I	GCGC	3 cut(s)	1185, 1224, 1394
HinP1I	GCGC	3 cut(s)	1185, 1224, 1394
HincII	GTYRAC	1 cut(s)	754
HindII	GTYRAC	1 cut(s)	754
HindIII	AAGCTT	1 cut(s)	1054
HpaI	GTTAAC	1 cut(s)	754
HpaII	CCGG	3 cut(s)	747, 1265, 2440
HphI	GGTGA	5 cut(s)	782, 1339, 1417, 1840, 2394
Hpy99I	CGWCG	1 cut(s)	389
HpyCH4III	ACNGT	3 cut(s)	671, 1258, 2080
HpyCH4IV	ACGT	7 cut(s)	109, 641, 827, 1239, 1251, 1780, 2150
HpyF10VI	GCNNNNNNNGC	8 cut(s)	35, 1113, 1122, 1184, 1274, 1283, 1393, 1475
HpyF3I	CTNAG	4 cut(s)	342, 809, 2318, 2675
HpySE526I	ACGT	7 cut(s)	109, 641, 827, 1239, 1251, 1780, 2150
Hsp92I	GRCGYC	1 cut(s)	1224
Hsp92II	CATG	9 cut(s)	288, 481, 523, 613, 840, 1791, 1990, 2036, 2349
HspAI	GCGC	3 cut(s)	1185, 1224, 1394
KasI	GGCGCC	1 cut(s)	1223
Ksp22I	TGATCA	6 cut(s)	145, 664, 1417, 1501, 1633, 2173
KspAI	GTTAAC	1 cut(s)	754
Kzo9I	GATC	7 cut(s)	145, 664, 1378, 1417, 1501, 1633, 2173
LguI	GCTCTTC	2 cut(s)	57, 1607
LmnI	GCTCC	6 cut(s)	43, 634, 1324, 1483, 2198, 2453
Lsp1109I	GCAGC	8 cut(s)	64, 86, 1116, 1270, 1420, 1543, 2354, 2625
LweI	GCATC	6 cut(s)	842, 1066, 1665, 1885, 2575, 2632
MaeI	CTAG	4 cut(s)	425, 2022, 2187, 2510
MaeII	ACGT	7 cut(s)	109, 641, 827, 1239, 1251, 1780, 2150
MaeIII	GTNAC	6 cut(s)	368, 770, 1033, 1252, 2542, 2678
MalI	GATC	7 cut(s)	147, 666, 1380, 1419, 1503, 1635, 2175
MboI	GATC	7 cut(s)	145, 664, 1378, 1417, 1501, 1633, 2173
MhlI	GDGCHC	2 cut(s)	957, 1279
Mly113I	GGCGCC	1 cut(s)	1224
MlyI	GAGTC	5 cut(s)	148, 1021, 1041, 1241, 2675
MmeI	TCCRAC	4 cut(s)	466, 1041, 1113, 1544
MroXI	GAANNNNTTC	1 cut(s)	655
MseI	TTAA	1 cut(s)	753
MslI	CAYNNNNRTG	3 cut(s)	679, 1991, 2188
MspA1I	CMGCKG	5 cut(s)	77, 178, 909, 1283, 2418
MspI	CCGG	3 cut(s)	747, 1265, 2440
MspR9I	CCNGG	7 cut(s)	748, 1122, 1265, 2273, 2338, 2631, 2647
Mva1269I	GAATGC	1 cut(s)	1125
MvaI	CCWGG	5 cut(s)	1122, 2273, 2338, 2631, 2647
MwoI	GCNNNNNNNGC	8 cut(s)	35, 1113, 1122, 1184, 1274, 1283, 1393, 1475
NarI	GGCGCC	1 cut(s)	1224
NciI	CCSGG	2 cut(s)	748, 1265
NdeII	GATC	7 cut(s)	145, 664, 1378, 1417, 1501, 1633, 2173
NlaIII	CATG	9 cut(s)	288, 481, 523, 613, 840, 1791, 1990, 2036, 2349
NlaIV	GGNNCC	2 cut(s)	1115, 1225
NmuCI	GTSAC	6 cut(s)	368, 770, 1033, 1252, 2542, 2678
NspI	RCATGY	2 cut(s)	481, 1990
NspV	TTCGAA	2 cut(s)	883, 2392
PaeI	GCATGC	2 cut(s)	481, 1990
PaeR7I	CTCGAG	1 cut(s)	1029
PagI	TCATGA	2 cut(s)	519, 609
PaqCI	CACCTGC	1 cut(s)	2131
PceI	AGGCCT	1 cut(s)	2020
PciSI	GCTCTTC	2 cut(s)	57, 1607
PctI	GAATGC	1 cut(s)	1125
PdmI	GAANNNNTTC	1 cut(s)	655
PfeI	GAWTC	8 cut(s)	440, 454, 516, 656, 1949, 1973, 2025, 2240
PflMI	CCANNNNNTGG	1 cut(s)	1180
PkrI	GCNGC	9 cut(s)	76, 79, 1106, 1285, 1435, 1558, 1670, 2344, 2615
PleI	GAGTC	5 cut(s)	148, 1021, 1040, 1240, 2675
PluTI	GGCGCC	1 cut(s)	1227
PpsI	GAGTC	5 cut(s)	148, 1021, 1040, 1240, 2675
PpuMI	RGGWCCY	1 cut(s)	1009
PshAI	GACNNNNGTC	1 cut(s)	1031
PsiI	TTATAA	1 cut(s)	561
Psp5II	RGGWCCY	1 cut(s)	1009
Psp6I	CCWGG	5 cut(s)	1120, 2271, 2336, 2629, 2645
PspGI	CCWGG	5 cut(s)	1120, 2271, 2336, 2629, 2645
PspN4I	GGNNCC	2 cut(s)	1115, 1225
PspPI	GGNCC	3 cut(s)	173, 1009, 2235
PspPPI	RGGWCCY	1 cut(s)	1009
PspXI	VCTCGAGB	1 cut(s)	1029
PvuII	CAGCTG	2 cut(s)	77, 1283
RsaI	GTAC	5 cut(s)	232, 272, 1648, 1735, 1775
RsaNI	GTAC	5 cut(s)	231, 271, 1647, 1734, 1774
RseI	CAYNNNNRTG	3 cut(s)	679, 1991, 2188
Rsr2I	CGGWCCG	1 cut(s)	173
RsrII	CGGWCCG	1 cut(s)	173
SapI	GCTCTTC	2 cut(s)	57, 1607
SaqAI	TTAA	1 cut(s)	753
SatI	GCNGC	9 cut(s)	75, 78, 1105, 1284, 1434, 1557, 1669, 2343, 2614
Sau3AI	GATC	7 cut(s)	145, 664, 1378, 1417, 1501, 1633, 2173
Sau96I	GGNCC	3 cut(s)	173, 1009, 2235
ScaI	AGTACT	1 cut(s)	1648
SchI	GAGTC	5 cut(s)	148, 1021, 1041, 1241, 2675
ScrFI	CCNGG	7 cut(s)	748, 1122, 1265, 2273, 2338, 2631, 2647
SduI	GDGCHC	2 cut(s)	957, 1279
SfaNI	GCATC	6 cut(s)	842, 1066, 1665, 1885, 2575, 2632
SfcI	CTRYAG	1 cut(s)	538
SfoI	GGCGCC	1 cut(s)	1225
Sfr274I	CTCGAG	1 cut(s)	1029
SfuI	TTCGAA	2 cut(s)	883, 2392
SinI	GGWCC	2 cut(s)	173, 1009
SlaI	CTCGAG	1 cut(s)	1029
SmiMI	CAYNNNNRTG	3 cut(s)	679, 1991, 2188
SmlI	CTYRAG	2 cut(s)	870, 1029
SmoI	CTYRAG	2 cut(s)	870, 1029
SphI	GCATGC	2 cut(s)	481, 1990
SseBI	AGGCCT	1 cut(s)	2020
SsiI	CCGC	7 cut(s)	171, 176, 909, 1592, 1668, 1671, 2418
SspDI	GGCGCC	1 cut(s)	1223
SspI	AATATT	1 cut(s)	1485
SspMI	CTAG	4 cut(s)	425, 2022, 2187, 2510
StuI	AGGCCT	1 cut(s)	2020
StyD4I	CCNGG	7 cut(s)	746, 1120, 1263, 2271, 2336, 2629, 2645
StyI	CCWWGG	3 cut(s)	377, 1886, 2037
TaaI	ACNGT	3 cut(s)	671, 1258, 2080
TaiI	ACGT	7 cut(s)	112, 644, 830, 1242, 1254, 1783, 2153
TaqII	GACCGA	1 cut(s)	1391
TatI	WGTACW	5 cut(s)	230, 270, 1646, 1733, 1773
TauI	GCSGC	1 cut(s)	1671
TfiI	GAWTC	8 cut(s)	440, 454, 516, 656, 1949, 1973, 2025, 2240
Tru1I	TTAA	1 cut(s)	753
Tru9I	TTAA	1 cut(s)	753
TscAI	CASTG	4 cut(s)	1185, 1831, 2432, 2683
TseFI	GTSAC	6 cut(s)	368, 770, 1033, 1252, 2542, 2678
TseI	GCWGC	8 cut(s)	74, 77, 1104, 1283, 1433, 1556, 2342, 2613
Tsp45I	GTSAC	6 cut(s)	368, 770, 1033, 1252, 2542, 2678
TspGWI	ACGGA	8 cut(s)	74, 255, 573, 577, 1233, 1311, 1758, 2086
TspRI	CASTG	4 cut(s)	1185, 1831, 2432, 2683
Van91I	CCANNNNNTGG	1 cut(s)	1180
VneI	GTGCAC	1 cut(s)	1275
VpaK11BI	GGWCC	2 cut(s)	173, 1009
XagI	CCTNNNNNAGG	1 cut(s)	2513
XapI	RAATTY	8 cut(s)	159, 404, 575, 922, 1656, 1881, 2263, 2286
XbaI	TCTAGA	1 cut(s)	2186
XceI	RCATGY	2 cut(s)	481, 1990
XhoI	CTCGAG	1 cut(s)	1029
XmiI	GTMKAC	1 cut(s)	621
XmnI	GAANNNNTTC	1 cut(s)	655
XspI	CTAG	4 cut(s)	425, 2022, 2187, 2510
ZrmI	AGTACT	1 cut(s)	1648

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.