pycom04g00670

Alpha-1,4-glucan-protein synthase UDP-forming

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0022537
Physical Location & Seq
Forward (+)
627695 .. 629092
1398 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g00670.2

Sequence Viewer

Length: 582 bp
ATGGCGCAGTACAGCAAGGTCGCCCCGACCCCTCTGCTGAAGGACGAGCTCGACATCGTCATCCCCACCAACCGAAACCTCGATTTCCTCGAGATGTGGAGGCCCTTCTTCCAGCAGTACCACCTCATCATCGTCCAGGACGGCGACCCGTCGAAGGTGATCAAGGTCCCGGAGGGCTTCGACTACGAGCTCTACAACCGCAACGATATCAACCGGATTCTGGGTCCCAAGGCGTCCTGCATCTCCTTCAAGGACTCTGCTTGCAGATGCTTCGGCTACATGGTCTCCAAGAAGAAGTACATCTTCACCATCGACGACGATTGCTTTGTTGCCAAAGATCCTTCTGGCAAAGATATCAATGCACTTGAGCAGCATATCAAGAACCTTCTGTCTCCGTCAACTCCATTTTTCGTCAGCAGGTGTGTGGTGCAGGGGAACCTTGAGATTGGAAGTCTCAGAGAAGTTGATGTTAAGTCTGGGCTTCCTGCCACTACAAGTACTGAAAGATTGGAACTTCTTGACGATGACGAGCATATTCTTAGCATTAAAATAATTGGTGGGGATCACAGACTTAGGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 map00520
Pfam Domains
Protein Families
RGP

Protein Analysis

194

Amino Acids

21.94

Weight (kDa)

5.59

Isoelectric Point (pI)

38.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022537)

Species Orthologous Gene IDs
pyrus_communis pycom04g00670 pycom04g08460 pycom07g03620 pycom10g01300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 408
Acc36I ACCTGC 1 cut(s) 408
AciI CCGC 1 cut(s) 199
AclWI GGATC 2 cut(s) 332, 570
AcuI CTGAAG 1 cut(s) 59
AcyI GRCGYC 1 cut(s) 233
AfaI GTAC 4 cut(s) 11, 119, 299, 499
AfiI CCNNNNNNNGG 2 cut(s) 154, 220
AgsI TTSAA 1 cut(s) 250
AjnI CCWGG 1 cut(s) 135
AluBI AGCT 2 cut(s) 49, 190
AluI AGCT 2 cut(s) 49, 190
Alw21I GWGCWC 2 cut(s) 51, 192
Alw26I GTCTC 3 cut(s) 289, 396, 458
AlwI GGATC 2 cut(s) 332, 570
Ama87I CYCGRG 1 cut(s) 89
AoxI GGCC 1 cut(s) 101
ApeKI GCWGC 1 cut(s) 370
ArsI GACNNNNNNTTYG 2 cut(s) 308, 340
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 3 cut(s) 102, 166, 224
AsuC2I CCSGG 1 cut(s) 170
AsuHPI GGTGA 2 cut(s) 169, 298
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 2 cut(s) 166, 224
BanII GRGCYC 2 cut(s) 51, 192
Bbv12I GWGCWC 2 cut(s) 51, 192
BbvI GCAGC 1 cut(s) 382
BccI CCATC 1 cut(s) 317
BceAI ACGGC 1 cut(s) 157
BcgI CGANNNNNNTGC 4 cut(s) 16, 50, 253, 287
BciT130I CCWGG 1 cut(s) 137
BclI TGATCA 1 cut(s) 159
BcnI CCSGG 1 cut(s) 170
BcoDI GTCTC 3 cut(s) 289, 396, 458
BfuAI ACCTGC 1 cut(s) 408
BisI GCNGC 1 cut(s) 371
BlsI GCNGC 1 cut(s) 372
BmcAI AGTACT 1 cut(s) 499
Bme1390I CCNGG 2 cut(s) 137, 170
Bme18I GGWCC 2 cut(s) 166, 224
BmeT110I CYCGRG 1 cut(s) 89
BmgT120I GGNCC 3 cut(s) 102, 166, 224
BmiI GGNNCC 4 cut(s) 168, 225, 226, 437
BmrFI CCNGG 2 cut(s) 137, 170
BmsI GCATC 2 cut(s) 249, 257
BpuEI CTTGAG 2 cut(s) 386, 461
BpuMI CCSGG 1 cut(s) 170
BsaHI GRCGYC 1 cut(s) 233
BsaI GGTCTC 1 cut(s) 289
BsaJI CCNNGG 1 cut(s) 228
BsaWI WCCGGW 1 cut(s) 213
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 220
BseBI CCWGG 1 cut(s) 137
BseDI CCNNGG 1 cut(s) 228
BseGI GGATG 1 cut(s) 60
BseLI CCNNNNNNNGG 2 cut(s) 154, 220
BseMII CTCAG 1 cut(s) 469
BseXI GCAGC 1 cut(s) 382
BsgI GTGCAG 1 cut(s) 449
BshFI GGCC 1 cut(s) 103
BsiHKAI GWGCWC 2 cut(s) 51, 192
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 2 cut(s) 170, 214
BslFI GGGAC 2 cut(s) 152, 210
BslI CCNNNNNNNGG 2 cut(s) 154, 220
BsmAI GTCTC 3 cut(s) 289, 396, 458
BsmFI GGGAC 2 cut(s) 152, 210
BsnI GGCC 1 cut(s) 103
Bso31I GGTCTC 1 cut(s) 289
BsoBI CYCGRG 1 cut(s) 89
Bsp1286I GDGCHC 2 cut(s) 51, 192
Bsp143I GATC 3 cut(s) 159, 337, 562
BspACI CCGC 1 cut(s) 199
BspANI GGCC 1 cut(s) 103
BspCNI CTCAG 1 cut(s) 468
BspLI GGNNCC 4 cut(s) 168, 225, 226, 437
BspMI ACCTGC 1 cut(s) 408
BspPI GGATC 2 cut(s) 332, 570
BspTNI GGTCTC 1 cut(s) 289
BssECI CCNNGG 1 cut(s) 228
BssMI GATC 3 cut(s) 159, 337, 562
BssNI GRCGYC 1 cut(s) 233
BssT1I CCWWGG 1 cut(s) 228
Bst2UI CCWGG 1 cut(s) 137
BstACI GRCGYC 1 cut(s) 233
BstC8I GCNNGC 1 cut(s) 262
BstDEI CTNAG 3 cut(s) 455, 539, 572
BstF5I GGATG 1 cut(s) 60
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 3 cut(s) 162, 340, 565
BstMAI GTCTC 3 cut(s) 289, 396, 458
BstMBI GATC 3 cut(s) 159, 337, 562
BstNI CCWGG 1 cut(s) 137
BstSCI CCNGG 2 cut(s) 135, 168
BstV1I GCAGC 1 cut(s) 382
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
BsuRI GGCC 1 cut(s) 103
BtsCI GGATG 1 cut(s) 60
BveI ACCTGC 1 cut(s) 408
Cac8I GCNNGC 1 cut(s) 262
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 3 cut(s) 102, 166, 224
CseI GACGC 1 cut(s) 222
Csp6I GTAC 4 cut(s) 10, 118, 298, 498
CviAII CATG 1 cut(s) 280
CviJI RGCY 6 cut(s) 49, 103, 177, 190, 276, 481
CviKI_1 RGCY 6 cut(s) 49, 103, 177, 190, 276, 481
CviQI GTAC 4 cut(s) 10, 118, 298, 498
DdeI CTNAG 3 cut(s) 455, 539, 572
DpnI GATC 3 cut(s) 161, 339, 564
DpnII GATC 3 cut(s) 159, 337, 562
Ecl136II GAGCTC 2 cut(s) 49, 190
Eco130I CCWWGG 1 cut(s) 228
Eco24I GRGCYC 2 cut(s) 51, 192
Eco31I GGTCTC 1 cut(s) 289
Eco32I GATATC 2 cut(s) 208, 355
Eco47I GGWCC 2 cut(s) 166, 224
Eco53kI GAGCTC 2 cut(s) 49, 190
Eco57I CTGAAG 1 cut(s) 59
Eco88I CYCGRG 1 cut(s) 89
EcoICRI GAGCTC 2 cut(s) 49, 190
EcoO109I RGGNCCY 3 cut(s) 102, 166, 224
EcoRII CCWGG 1 cut(s) 135
EcoRV GATATC 2 cut(s) 208, 355
EcoT14I CCWWGG 1 cut(s) 228
EcoT38I GRGCYC 2 cut(s) 51, 192
ErhI CCWWGG 1 cut(s) 228
FaeI CATG 1 cut(s) 283
FaiI YATR 4 cut(s) 281, 375, 534, 580
FalI AAGNNNNNCTT 2 cut(s) 287, 319
FaqI GGGAC 2 cut(s) 152, 210
FatI CATG 1 cut(s) 279
FbaI TGATCA 1 cut(s) 159
Fnu4HI GCNGC 1 cut(s) 371
FokI GGATG 1 cut(s) 47
FriOI GRGCYC 2 cut(s) 51, 192
Fsp4HI GCNGC 1 cut(s) 371
GlaI GCGC 1 cut(s) 6
GluI GCNGC 1 cut(s) 371
HaeIII GGCC 1 cut(s) 103
HapII CCGG 2 cut(s) 170, 214
HgaI GACGC 1 cut(s) 222
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 233
Hin1II CATG 1 cut(s) 283
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 399
HindII GTYRAC 1 cut(s) 399
HinfI GANTC 2 cut(s) 217, 254
HpaII CCGG 2 cut(s) 170, 214
HphI GGTGA 2 cut(s) 169, 298
Hpy166II GTNNAC 1 cut(s) 399
Hpy188I TCNGA 1 cut(s) 458
Hpy188III TCNNGA 3 cut(s) 91, 379, 518
Hpy8I GTNNAC 1 cut(s) 399
Hpy99I CGWCG 3 cut(s) 154, 317, 320
HpyAV CCTTC 6 cut(s) 34, 115, 148, 256, 351, 395
HpyCH4V TGCA 4 cut(s) 240, 264, 362, 430
HpyF3I CTNAG 3 cut(s) 455, 539, 572
Hsp92I GRCGYC 1 cut(s) 233
Hsp92II CATG 1 cut(s) 283
HspAI GCGC 1 cut(s) 5
KflI GGGWCCC 1 cut(s) 224
Ksp22I TGATCA 1 cut(s) 159
Kzo9I GATC 3 cut(s) 159, 337, 562
Lsp1109I GCAGC 1 cut(s) 382
LweI GCATC 2 cut(s) 249, 257
MalI GATC 3 cut(s) 161, 339, 564
MboI GATC 3 cut(s) 159, 337, 562
MboII GAAGA 3 cut(s) 100, 295, 304
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 2 cut(s) 51, 192
MluCI AATT 1 cut(s) 552
MlyI GAGTC 1 cut(s) 248
MnlI CCTC 6 cut(s) 42, 89, 93, 98, 134, 166
MseI TTAA 2 cut(s) 471, 546
MspI CCGG 2 cut(s) 170, 214
MspR9I CCNGG 2 cut(s) 137, 170
MvaI CCWGG 1 cut(s) 137
NciI CCSGG 1 cut(s) 170
NdeII GATC 3 cut(s) 159, 337, 562
NlaIII CATG 1 cut(s) 283
NlaIV GGNNCC 4 cut(s) 168, 225, 226, 437
PaeR7I CTCGAG 1 cut(s) 89
PaqCI CACCTGC 1 cut(s) 408
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PfeI GAWTC 1 cut(s) 217
PflFI GACNNNGTC 1 cut(s) 56
PfoI TCCNGGA 2 cut(s) 135, 168
PkrI GCNGC 1 cut(s) 372
PleI GAGTC 1 cut(s) 248
PpsI GAGTC 1 cut(s) 248
PpuMI RGGWCCY 2 cut(s) 166, 224
Psp124BI GAGCTC 2 cut(s) 51, 192
Psp5II RGGWCCY 2 cut(s) 166, 224
Psp6I CCWGG 1 cut(s) 135
PspGI CCWGG 1 cut(s) 135
PspN4I GGNNCC 4 cut(s) 168, 225, 226, 437
PspPI GGNCC 3 cut(s) 102, 166, 224
PspPPI RGGWCCY 2 cut(s) 166, 224
PsuI RGATCY 1 cut(s) 337
PsyI GACNNNGTC 1 cut(s) 56
RsaI GTAC 4 cut(s) 11, 119, 299, 499
RsaNI GTAC 4 cut(s) 10, 118, 298, 498
SacI GAGCTC 2 cut(s) 51, 192
SaqAI TTAA 2 cut(s) 471, 546
SatI GCNGC 1 cut(s) 371
Sau3AI GATC 3 cut(s) 159, 337, 562
Sau96I GGNCC 3 cut(s) 102, 166, 224
ScaI AGTACT 1 cut(s) 499
SchI GAGTC 1 cut(s) 248
ScrFI CCNGG 2 cut(s) 137, 170
SduI GDGCHC 2 cut(s) 51, 192
SfaNI GCATC 2 cut(s) 249, 257
Sfr274I CTCGAG 1 cut(s) 89
SinI GGWCC 2 cut(s) 166, 224
SlaI CTCGAG 1 cut(s) 89
SmlI CTYRAG 3 cut(s) 89, 365, 440
SmoI CTYRAG 3 cut(s) 89, 365, 440
Sse9I AATT 1 cut(s) 552
SsiI CCGC 1 cut(s) 199
SstI GAGCTC 2 cut(s) 51, 192
StyD4I CCNGG 2 cut(s) 135, 168
StyI CCWWGG 1 cut(s) 228
TaqI TCGA 6 cut(s) 51, 81, 90, 152, 180, 312
TasI AATT 1 cut(s) 552
TatI WGTACW 3 cut(s) 9, 297, 497
TfiI GAWTC 1 cut(s) 217
Tru1I TTAA 2 cut(s) 471, 546
Tru9I TTAA 2 cut(s) 471, 546
TseI GCWGC 1 cut(s) 370
TspGWI ACGGA 1 cut(s) 384
Tth111I GACNNNGTC 1 cut(s) 56
VpaK11BI GGWCC 2 cut(s) 166, 224
XhoI CTCGAG 1 cut(s) 89
ZrmI AGTACT 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.