pycom04g06500

Peptidase inhibitor I9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0028671
Physical Location & Seq
Reverse (-)
6521537 .. 6522035
499 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 459 bp
ATGGCTCGTCATGCTCACTTGGCAATGTACAAAGTCTGTGAGTTTGATTGTTCTGAAGGTTATGTTTTGGCTGCAATGGATGTTGTGGTTGATGATGGAGTGGACGTGTTATCCTTCTCACGTGTTGCATTTGGAGCAACTCAAAAGGGAGTTTTTGTCAACTCTGCAGCTGGAAACTTCGGTCTTGAACCCGATTCTTTGTCAAATGAGCCCTCATGGATTCTCACAGTTGGAACAAGAACAACTGACATAATATTAAACATAAACTCACAACCTACAGCCACAATCTTGTCCAATGGCAACAGAGACGCCCTTGATCCCGAGATTGCATCTTTTTCATCAAGAGGGCCAAACACTACAAGCCACGAAATTCTGCAACCTAACATCATTGGACCTGGTGTTGACATCCTGGCAACTATTAACAACCGAGATGGATTCCATAGAAATCAAGAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

153

Amino Acids

16.38

Weight (kDa)

4.75

Isoelectric Point (pI)

35.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028671)

Species Orthologous Gene IDs
malus_domestica MD04G1071600.v1.1
pyrus_communis pycom04g06500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 311
AcsI RAATTY 1 cut(s) 369
AcuI CTGAAG 1 cut(s) 75
AcvI CACGTG 1 cut(s) 122
AcyI GRCGYC 1 cut(s) 309
AfaI GTAC 1 cut(s) 29
AflIII ACRYGT 2 cut(s) 105, 121
AgsI TTSAA 1 cut(s) 188
AjiI CACGTC 1 cut(s) 106
AjnI CCWGG 2 cut(s) 394, 408
AluBI AGCT 1 cut(s) 170
AluI AGCT 1 cut(s) 170
Alw26I GTCTC 1 cut(s) 300
AlwI GGATC 1 cut(s) 311
Ama87I CYCGRG 1 cut(s) 320
AoxI GGCC 1 cut(s) 347
ApeKI GCWGC 2 cut(s) 71, 167
ApoI RAATTY 1 cut(s) 369
AspS9I GGNCC 2 cut(s) 347, 392
AvaI CYCGRG 1 cut(s) 320
AvaII GGWCC 1 cut(s) 392
BanII GRGCYC 1 cut(s) 213
BbrPI CACGTG 1 cut(s) 122
BbvI GCAGC 2 cut(s) 58, 179
BccI CCATC 2 cut(s) 89, 425
BciT130I CCWGG 2 cut(s) 396, 410
BcoDI GTCTC 1 cut(s) 300
BfmI CTRYAG 2 cut(s) 165, 276
BisI GCNGC 2 cut(s) 72, 168
BlsI GCNGC 2 cut(s) 73, 169
Bme1390I CCNGG 2 cut(s) 396, 410
Bme18I GGWCC 1 cut(s) 392
BmeT110I CYCGRG 1 cut(s) 320
BmgBI CACGTC 1 cut(s) 106
BmgT120I GGNCC 2 cut(s) 347, 392
BmrFI CCNGG 2 cut(s) 396, 410
BmsI GCATC 1 cut(s) 338
BsaAI YACGTR 1 cut(s) 122
BsaHI GRCGYC 1 cut(s) 309
BsaXI ACNNNNNCTCC 4 cut(s) 90, 120, 141, 171
Bse3DI GCAATG 2 cut(s) 30, 81
BseBI CCWGG 2 cut(s) 396, 410
BseGI GGATG 2 cut(s) 85, 405
BseMI GCAATG 2 cut(s) 30, 81
BseXI GCAGC 2 cut(s) 58, 179
BshFI GGCC 1 cut(s) 349
BsiHKCI CYCGRG 1 cut(s) 320
BsmAI GTCTC 1 cut(s) 300
BsmBI CGTCTC 1 cut(s) 300
BsnI GGCC 1 cut(s) 349
BsoBI CYCGRG 1 cut(s) 320
Bsp1286I GDGCHC 1 cut(s) 213
Bsp1407I TGTACA 1 cut(s) 27
Bsp143I GATC 1 cut(s) 316
BspANI GGCC 1 cut(s) 349
BspMAI CTGCAG 1 cut(s) 169
BspPI GGATC 1 cut(s) 311
BsrDI GCAATG 2 cut(s) 30, 81
BsrGI TGTACA 1 cut(s) 27
BssMI GATC 1 cut(s) 316
BssNI GRCGYC 1 cut(s) 309
Bst2UI CCWGG 2 cut(s) 396, 410
Bst4CI ACNGT 1 cut(s) 229
BstACI GRCGYC 1 cut(s) 309
BstAUI TGTACA 1 cut(s) 27
BstBAI YACGTR 1 cut(s) 122
BstF5I GGATG 2 cut(s) 85, 405
BstKTI GATC 1 cut(s) 319
BstMAI GTCTC 1 cut(s) 300
BstMBI GATC 1 cut(s) 316
BstMWI GCNNNNNNNGC 3 cut(s) 11, 20, 134
BstNI CCWGG 2 cut(s) 396, 410
BstSCI CCNGG 2 cut(s) 394, 408
BstSFI CTRYAG 2 cut(s) 165, 276
BstV1I GCAGC 2 cut(s) 58, 179
BsuRI GGCC 1 cut(s) 349
BtrI CACGTC 1 cut(s) 106
BtsCI GGATG 2 cut(s) 85, 405
Cfr13I GGNCC 2 cut(s) 347, 392
CseI GACGC 1 cut(s) 317
CsiI ACCWGGT 1 cut(s) 394
Csp6I GTAC 1 cut(s) 28
CviAII CATG 2 cut(s) 11, 216
CviJI RGCY 7 cut(s) 5, 71, 170, 211, 281, 349, 363
CviKI_1 RGCY 7 cut(s) 5, 71, 170, 211, 281, 349, 363
CviQI GTAC 1 cut(s) 28
DpnI GATC 1 cut(s) 318
DpnII GATC 1 cut(s) 316
Eco24I GRGCYC 1 cut(s) 213
Eco47I GGWCC 1 cut(s) 392
Eco57I CTGAAG 1 cut(s) 75
Eco72I CACGTG 1 cut(s) 122
Eco88I CYCGRG 1 cut(s) 320
EcoRII CCWGG 2 cut(s) 394, 408
EcoT38I GRGCYC 1 cut(s) 213
Esp3I CGTCTC 1 cut(s) 300
FaeI CATG 2 cut(s) 14, 219
FaiI YATR 6 cut(s) 12, 63, 217, 251, 263, 441
FatI CATG 2 cut(s) 10, 215
Fnu4HI GCNGC 2 cut(s) 72, 168
FokI GGATG 2 cut(s) 92, 392
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 2 cut(s) 72, 168
GluI GCNGC 2 cut(s) 72, 168
HaeIII GGCC 1 cut(s) 349
HgaI GACGC 1 cut(s) 317
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 2 cut(s) 14, 219
HincII GTYRAC 2 cut(s) 160, 403
HindII GTYRAC 2 cut(s) 160, 403
HinfI GANTC 3 cut(s) 194, 220, 435
Hpy166II GTNNAC 3 cut(s) 103, 160, 403
Hpy188I TCNGA 1 cut(s) 55
Hpy188III TCNNGA 4 cut(s) 185, 320, 342, 449
Hpy8I GTNNAC 3 cut(s) 103, 160, 403
HpyAV CCTTC 2 cut(s) 50, 124
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4IV ACGT 2 cut(s) 105, 121
HpyCH4V TGCA 5 cut(s) 74, 128, 167, 329, 376
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 20, 134
HpySE526I ACGT 2 cut(s) 105, 121
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 2 cut(s) 14, 219
Kzo9I GATC 1 cut(s) 316
LmnI GCTCC 1 cut(s) 134
LpnPI CCDG 5 cut(s) 156, 381, 395, 408, 422
Lsp1109I GCAGC 2 cut(s) 58, 179
LweI GCATC 1 cut(s) 338
MabI ACCWGGT 1 cut(s) 394
MaeII ACGT 2 cut(s) 105, 121
MalI GATC 1 cut(s) 318
MboI GATC 1 cut(s) 316
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 1 cut(s) 369
MmeI TCCRAC 1 cut(s) 211
MnlI CCTC 2 cut(s) 223, 338
MseI TTAA 2 cut(s) 257, 420
MspA1I CMGCKG 1 cut(s) 170
MspR9I CCNGG 2 cut(s) 396, 410
MvaI CCWGG 2 cut(s) 396, 410
MwoI GCNNNNNNNGC 3 cut(s) 11, 20, 134
NdeII GATC 1 cut(s) 316
NlaIII CATG 2 cut(s) 14, 219
PfeI GAWTC 3 cut(s) 194, 220, 435
PkrI GCNGC 2 cut(s) 73, 169
PmaCI CACGTG 1 cut(s) 122
PmlI CACGTG 1 cut(s) 122
Ppu21I YACGTR 1 cut(s) 122
Psp6I CCWGG 2 cut(s) 394, 408
PspCI CACGTG 1 cut(s) 122
PspGI CCWGG 2 cut(s) 394, 408
PspPI GGNCC 2 cut(s) 347, 392
PstI CTGCAG 1 cut(s) 169
PvuII CAGCTG 1 cut(s) 170
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
SaqAI TTAA 2 cut(s) 257, 420
SatI GCNGC 2 cut(s) 72, 168
Sau3AI GATC 1 cut(s) 316
Sau96I GGNCC 2 cut(s) 347, 392
ScrFI CCNGG 2 cut(s) 396, 410
SduI GDGCHC 1 cut(s) 213
SetI ASST 7 cut(s) 61, 108, 124, 172, 277, 382, 397
SexAI ACCWGGT 1 cut(s) 394
SfaNI GCATC 1 cut(s) 338
SfcI CTRYAG 2 cut(s) 165, 276
SinI GGWCC 1 cut(s) 392
Sse9I AATT 1 cut(s) 369
SspI AATATT 1 cut(s) 255
StyD4I CCNGG 2 cut(s) 394, 408
TaaI ACNGT 1 cut(s) 229
TaiI ACGT 2 cut(s) 108, 124
TaqII GACCGA 1 cut(s) 170
TasI AATT 1 cut(s) 369
TatI WGTACW 1 cut(s) 27
TfiI GAWTC 3 cut(s) 194, 220, 435
Tru1I TTAA 2 cut(s) 257, 420
Tru9I TTAA 2 cut(s) 257, 420
TseI GCWGC 2 cut(s) 71, 167
TspDTI ATGAA 1 cut(s) 327
VpaK11BI GGWCC 1 cut(s) 392
XapI RAATTY 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.