pycom04g19000

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0028740
Physical Location & Seq
Forward (+)
20821735 .. 20824046
2312 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g19000.3

Sequence Viewer

Length: 936 bp
ATGGGATCGGGTTCCGAAACAGCGTCGCTAAAAATTCCTATCATAGATTTCACAAATAGTGGCAGTGGCACCACGCCAGACCAAGTAAGACATGCACTTGAAGAGTACGGTTGTTTCGTGGCGGCGTATGACAGAGTTCCGGCTCAACTTGTTGACAAGGTAATGGCCCAGTCTAAGGATCTGTTTGATCTGCCCACAGAAACCAAACTTAGCCACACTGGCGACGACGTACTCCGTGGATATATTGGACCAAGTCCTAGGCTTCCTTACTCCGAGACCTTTGCGATTAGTCATGCTACAAGCTTCCAAGATGTGCAGAATTTCATGAACCTCGTGTGGCCTGCTGGCAAAGATAACTTCTGTGAAACTACTCACAGTTATGTGAAGTTGATAGAAGAGTTGGGTGAACGGGTGATGCGATTGTTGTTCGAAAGCTATGGTGTTGCAACGAAGCACTATGAGTCCTTTGCTGCTTTCAATGACCACGTTTTTCGATTAATCAAGTATAGAGAAATGAAAGACATCGACATCACTGGTACTAGTGTTTGGTTTCCAAACCATAGCGACGCAAGCTTCATATCAATACTTTTCCAGCACCAAATTGGCGGTTTAGAAATCAAATCCAAGGATGGAACCTTTGTGGGTATTGACGCCTCACCTGGCCACTTCATAGTCTTCGCAGGAGACATGTTGCAGATTTGGAGTAACCAGAGAATAAAAGCTTGCAACCATCGCGTCAGGATTTGGGACGTGAATGTGGAAAGGTGCTCAGTGGGAATTTTCATATTCAACAGGGGAACAATACAAGCACCAGAAGAAGTAACCGATGAGAACCACCCATTGTGCTACAAGCCATGTGATGGTCTTGGCTATATTACTTTTGCTTTCTCCAACCTGGATATGACCGGTCGTATCAAGGCCTACTGTGGTATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

312

Amino Acids

34.91

Weight (kDa)

5.54

Isoelectric Point (pI)

23.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028740)

Species Orthologous Gene IDs
malus_domestica MD00G1052600.v1.1
pyrus_communis pycom04g19000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 68
AccB7I CCANNNNNTGG 1 cut(s) 860
AccII CGCG 1 cut(s) 735
AciI CCGC 2 cut(s) 122, 606
AclWI GGATC 2 cut(s) 13, 186
AcoI YGGCCR 1 cut(s) 661
AcsI RAATTY 3 cut(s) 33, 319, 777
AcyI GRCGYC 1 cut(s) 651
AfaI GTAC 3 cut(s) 107, 231, 538
AfiI CCNNNNNNNGG 2 cut(s) 175, 860
AflIII ACRYGT 1 cut(s) 687
AgeI ACCGGT 1 cut(s) 905
AgsI TTSAA 3 cut(s) 101, 478, 790
AhlI ACTAGT 1 cut(s) 539
AjiI CACGTC 1 cut(s) 751
AjnI CCWGG 2 cut(s) 658, 894
AluBI AGCT 4 cut(s) 303, 435, 573, 722
AluI AGCT 4 cut(s) 303, 435, 573, 722
Alw21I GWGCWC 1 cut(s) 770
Alw26I GTCTC 2 cut(s) 269, 678
AlwI GGATC 2 cut(s) 13, 186
AoxI GGCC 4 cut(s) 165, 338, 661, 918
ApeKI GCWGC 1 cut(s) 470
ApoI RAATTY 3 cut(s) 33, 319, 777
AseI ATTAAT 1 cut(s) 497
AsiGI ACCGGT 1 cut(s) 905
AspA2I CCTAGG 1 cut(s) 257
AspS9I GGNCC 2 cut(s) 166, 248
AsuHPI GGTGA 3 cut(s) 416, 424, 648
AsuII TTCGAA 1 cut(s) 429
AvaII GGWCC 1 cut(s) 248
AvrII CCTAGG 1 cut(s) 257
BaeI ACNNNNGTAYC 3 cut(s) 528, 561, 913
BalI TGGCCA 1 cut(s) 663
BanI GGYRCC 1 cut(s) 68
BauI CACGAG 1 cut(s) 332
BbsI GAAGAC 1 cut(s) 667
Bbv12I GWGCWC 1 cut(s) 770
BbvI GCAGC 1 cut(s) 457
BccI CCATC 3 cut(s) 623, 738, 854
BcgI CGANNNNNNTGC 2 cut(s) 263, 297
BciT130I CCWGG 2 cut(s) 660, 896
BcoDI GTCTC 2 cut(s) 269, 678
BcuI ACTAGT 1 cut(s) 539
BfaI CTAG 2 cut(s) 258, 540
BglI GCCNNNNNGGC 1 cut(s) 219
BisI GCNGC 2 cut(s) 123, 471
BlnI CCTAGG 1 cut(s) 257
BlsI GCNGC 2 cut(s) 124, 472
Bme1390I CCNGG 2 cut(s) 660, 896
Bme18I GGWCC 1 cut(s) 248
BmgBI CACGTC 1 cut(s) 751
BmgT120I GGNCC 2 cut(s) 166, 248
BmiI GGNNCC 3 cut(s) 13, 70, 634
BmrFI CCNGG 2 cut(s) 660, 896
BmrI ACTGGG 1 cut(s) 163
BmsI GCATC 1 cut(s) 405
BmuI ACTGGG 1 cut(s) 163
BpiI GAAGAC 1 cut(s) 667
Bpu14I TTCGAA 1 cut(s) 429
BsaHI GRCGYC 1 cut(s) 651
BsaI GGTCTC 1 cut(s) 269
BsaJI CCNNGG 3 cut(s) 235, 257, 624
BsaWI WCCGGW 1 cut(s) 905
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 860
Bse118I RCCGGY 1 cut(s) 905
Bse1I ACTGG 3 cut(s) 169, 223, 538
BseBI CCWGG 2 cut(s) 660, 896
BseDI CCNNGG 3 cut(s) 235, 257, 624
BseGI GGATG 1 cut(s) 634
BseLI CCNNNNNNNGG 2 cut(s) 175, 860
BseMII CTCAG 1 cut(s) 783
BseNI ACTGG 3 cut(s) 169, 223, 538
BseXI GCAGC 1 cut(s) 457
BsgI GTGCAG 1 cut(s) 335
Bsh1236I CGCG 1 cut(s) 735
Bsh1285I CGRYCG 1 cut(s) 910
BshFI GGCC 4 cut(s) 167, 340, 663, 920
BshNI GGYRCC 1 cut(s) 68
BshTI ACCGGT 1 cut(s) 905
BsiEI CGRYCG 1 cut(s) 910
BsiHKAI GWGCWC 1 cut(s) 770
BsiSI CCGG 2 cut(s) 140, 906
BslFI GGGAC 1 cut(s) 761
BslI CCNNNNNNNGG 2 cut(s) 175, 860
BsmAI GTCTC 2 cut(s) 269, 678
BsmFI GGGAC 1 cut(s) 761
BsnI GGCC 4 cut(s) 167, 340, 663, 920
Bso31I GGTCTC 1 cut(s) 269
Bsp119I TTCGAA 1 cut(s) 429
Bsp1286I GDGCHC 1 cut(s) 770
Bsp143I GATC 3 cut(s) 5, 178, 187
BspACI CCGC 2 cut(s) 122, 606
BspANI GGCC 4 cut(s) 167, 340, 663, 920
BspCNI CTCAG 1 cut(s) 782
BspFNI CGCG 1 cut(s) 735
BspHI TCATGA 1 cut(s) 324
BspLI GGNNCC 3 cut(s) 13, 70, 634
BspPI GGATC 2 cut(s) 13, 186
BspT104I TTCGAA 1 cut(s) 429
BspT107I GGYRCC 1 cut(s) 68
BspTNI GGTCTC 1 cut(s) 269
BsrFI RCCGGY 1 cut(s) 905
BsrI ACTGG 3 cut(s) 169, 223, 538
BssAI RCCGGY 1 cut(s) 905
BssECI CCNNGG 3 cut(s) 235, 257, 624
BssMI GATC 3 cut(s) 5, 178, 187
BssNI GRCGYC 1 cut(s) 651
BssSI CACGAG 1 cut(s) 332
BssT1I CCWWGG 2 cut(s) 257, 624
Bst2BI CACGAG 1 cut(s) 332
Bst2UI CCWGG 2 cut(s) 660, 896
Bst4CI ACNGT 3 cut(s) 110, 377, 926
Bst6I CTCTTC 2 cut(s) 96, 390
BstACI GRCGYC 1 cut(s) 651
BstBI TTCGAA 1 cut(s) 429
BstC8I GCNNGC 4 cut(s) 342, 346, 571, 724
BstDEI CTNAG 3 cut(s) 174, 209, 769
BstDSI CCRYGG 1 cut(s) 235
BstF5I GGATG 1 cut(s) 634
BstFNI CGCG 1 cut(s) 735
BstKTI GATC 3 cut(s) 8, 181, 190
BstMAI GTCTC 2 cut(s) 269, 678
BstMBI GATC 3 cut(s) 5, 178, 187
BstMCI CGRYCG 1 cut(s) 910
BstMWI GCNNNNNNNGC 3 cut(s) 219, 570, 732
BstNI CCWGG 2 cut(s) 660, 896
BstNSI RCATGY 2 cut(s) 95, 691
BstSCI CCNGG 2 cut(s) 658, 894
BstUI CGCG 1 cut(s) 735
BstV1I GCAGC 1 cut(s) 457
BstV2I GAAGAC 1 cut(s) 667
BstX2I RGATCY 1 cut(s) 178
BstYI RGATCY 1 cut(s) 178
BsuRI GGCC 4 cut(s) 167, 340, 663, 920
BtgI CCRYGG 1 cut(s) 235
BtgZI GCGATG 1 cut(s) 716
BtrI CACGTC 1 cut(s) 751
BtsCI GGATG 1 cut(s) 634
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 4 cut(s) 70, 216, 531, 777
Cac8I GCNNGC 4 cut(s) 342, 346, 571, 724
CciI TCATGA 1 cut(s) 324
Cfr10I RCCGGY 1 cut(s) 905
Cfr13I GGNCC 2 cut(s) 166, 248
CseI GACGC 4 cut(s) 12, 575, 659, 724
Csp6I GTAC 3 cut(s) 106, 230, 537
CspAI ACCGGT 1 cut(s) 905
CviAII CATG 5 cut(s) 92, 293, 325, 688, 855
CviQI GTAC 3 cut(s) 106, 230, 537
DdeI CTNAG 3 cut(s) 174, 209, 769
DpnI GATC 3 cut(s) 7, 180, 189
DpnII GATC 3 cut(s) 5, 178, 187
EaeI YGGCCR 1 cut(s) 661
Eam1104I CTCTTC 2 cut(s) 96, 390
EarI CTCTTC 2 cut(s) 96, 390
Eco130I CCWWGG 2 cut(s) 257, 624
Eco147I AGGCCT 1 cut(s) 920
Eco31I GGTCTC 1 cut(s) 269
Eco47I GGWCC 1 cut(s) 248
EcoRII CCWGG 2 cut(s) 658, 894
EcoT14I CCWWGG 2 cut(s) 257, 624
ErhI CCWWGG 2 cut(s) 257, 624
FaeI CATG 5 cut(s) 95, 296, 328, 691, 858
FaqI GGGAC 1 cut(s) 761
FatI CATG 5 cut(s) 91, 292, 324, 687, 854
Fnu4HI GCNGC 2 cut(s) 123, 471
FokI GGATG 1 cut(s) 641
Fsp4HI GCNGC 2 cut(s) 123, 471
FspBI CTAG 2 cut(s) 258, 540
GluI GCNGC 2 cut(s) 123, 471
HaeIII GGCC 4 cut(s) 167, 340, 663, 920
HapII CCGG 2 cut(s) 140, 906
HgaI GACGC 4 cut(s) 12, 575, 659, 724
Hin1I GRCGYC 1 cut(s) 651
Hin1II CATG 5 cut(s) 95, 296, 328, 691, 858
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HindIII AAGCTT 3 cut(s) 301, 571, 720
HinfI GANTC 1 cut(s) 461
HpaII CCGG 2 cut(s) 140, 906
HphI GGTGA 3 cut(s) 416, 424, 648
Hpy166II GTNNAC 2 cut(s) 154, 407
Hpy188I TCNGA 3 cut(s) 16, 274, 935
Hpy188III TCNNGA 2 cut(s) 325, 739
Hpy8I GTNNAC 2 cut(s) 154, 407
Hpy99I CGWCG 4 cut(s) 28, 227, 230, 569
HpyCH4III ACNGT 3 cut(s) 110, 377, 926
HpyCH4IV ACGT 3 cut(s) 228, 486, 750
HpyCH4V TGCA 5 cut(s) 95, 316, 446, 694, 726
HpyF10VI GCNNNNNNNGC 3 cut(s) 219, 570, 732
HpyF3I CTNAG 3 cut(s) 174, 209, 769
HpySE526I ACGT 3 cut(s) 228, 486, 750
Hsp92I GRCGYC 1 cut(s) 651
Hsp92II CATG 5 cut(s) 95, 296, 328, 691, 858
Kzo9I GATC 3 cut(s) 5, 178, 187
Lsp1109I GCAGC 1 cut(s) 457
LweI GCATC 1 cut(s) 405
MaeI CTAG 2 cut(s) 258, 540
MaeII ACGT 3 cut(s) 228, 486, 750
MaeIII GTNAC 2 cut(s) 704, 820
MalI GATC 3 cut(s) 7, 180, 189
MboI GATC 3 cut(s) 5, 178, 187
MboII GAAGA 4 cut(s) 113, 407, 667, 827
MflI RGATCY 1 cut(s) 178
MhlI GDGCHC 1 cut(s) 770
MlsI TGGCCA 1 cut(s) 663
MluCI AATT 4 cut(s) 33, 319, 600, 777
MluNI TGGCCA 1 cut(s) 663
MlyI GAGTC 1 cut(s) 470
MmeI TCCRAC 1 cut(s) 915
MnlI CCTC 2 cut(s) 341, 664
Mox20I TGGCCA 1 cut(s) 663
MscI TGGCCA 1 cut(s) 663
MseI TTAA 1 cut(s) 497
MslI CAYNNNNRTG 1 cut(s) 378
Msp20I TGGCCA 1 cut(s) 663
MspI CCGG 2 cut(s) 140, 906
MspR9I CCNGG 2 cut(s) 660, 896
MvaI CCWGG 2 cut(s) 660, 896
MvnI CGCG 1 cut(s) 735
MwoI GCNNNNNNNGC 3 cut(s) 219, 570, 732
NdeII GATC 3 cut(s) 5, 178, 187
NlaIII CATG 5 cut(s) 95, 296, 328, 691, 858
NlaIV GGNNCC 3 cut(s) 13, 70, 634
NspI RCATGY 2 cut(s) 95, 691
NspV TTCGAA 1 cut(s) 429
PagI TCATGA 1 cut(s) 324
PceI AGGCCT 1 cut(s) 920
PciI ACATGT 1 cut(s) 687
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 415
PflFI GACNNNGTC 1 cut(s) 252
PflMI CCANNNNNTGG 1 cut(s) 860
PinAI ACCGGT 1 cut(s) 905
PkrI GCNGC 2 cut(s) 124, 472
PleI GAGTC 1 cut(s) 469
PpsI GAGTC 1 cut(s) 469
PscI ACATGT 1 cut(s) 687
PshBI ATTAAT 1 cut(s) 497
Psp6I CCWGG 2 cut(s) 658, 894
PspGI CCWGG 2 cut(s) 658, 894
PspN4I GGNNCC 3 cut(s) 13, 70, 634
PspPI GGNCC 2 cut(s) 166, 248
PsuI RGATCY 1 cut(s) 178
PsyI GACNNNGTC 1 cut(s) 252
RsaI GTAC 3 cut(s) 107, 231, 538
RsaNI GTAC 3 cut(s) 106, 230, 537
RseI CAYNNNNRTG 1 cut(s) 378
SaqAI TTAA 1 cut(s) 497
SatI GCNGC 2 cut(s) 123, 471
Sau3AI GATC 3 cut(s) 5, 178, 187
Sau96I GGNCC 2 cut(s) 166, 248
SchI GAGTC 1 cut(s) 470
ScrFI CCNGG 2 cut(s) 660, 896
SduI GDGCHC 1 cut(s) 770
SfaNI GCATC 1 cut(s) 405
SfuI TTCGAA 1 cut(s) 429
SinI GGWCC 1 cut(s) 248
SmiMI CAYNNNNRTG 1 cut(s) 378
SpeI ACTAGT 1 cut(s) 539
Sse9I AATT 4 cut(s) 33, 319, 600, 777
SseBI AGGCCT 1 cut(s) 920
SsiI CCGC 2 cut(s) 122, 606
SspMI CTAG 2 cut(s) 258, 540
StuI AGGCCT 1 cut(s) 920
StyD4I CCNGG 2 cut(s) 658, 894
StyI CCWWGG 2 cut(s) 257, 624
TaaI ACNGT 3 cut(s) 110, 377, 926
TaiI ACGT 3 cut(s) 231, 489, 753
TaqI TCGA 3 cut(s) 429, 493, 525
TasI AATT 4 cut(s) 33, 319, 600, 777
TauI GCSGC 1 cut(s) 125
Tru1I TTAA 1 cut(s) 497
Tru9I TTAA 1 cut(s) 497
TscAI CASTG 4 cut(s) 70, 223, 538, 777
TseI GCWGC 1 cut(s) 470
TspDTI ATGAA 6 cut(s) 313, 341, 530, 565, 658, 772
TspGWI ACGGA 1 cut(s) 224
TspRI CASTG 4 cut(s) 70, 223, 538, 777
Tth111I GACNNNGTC 1 cut(s) 252
Van91I CCANNNNNTGG 1 cut(s) 860
VpaK11BI GGWCC 1 cut(s) 248
VspI ATTAAT 1 cut(s) 497
XapI RAATTY 3 cut(s) 33, 319, 777
XceI RCATGY 2 cut(s) 95, 691
XcmI CCANNNNNNNNNTGG 1 cut(s) 599
XmaJI CCTAGG 1 cut(s) 257
XspI CTAG 2 cut(s) 258, 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.