pycom05g05340

Pentatricopeptide repeat-containing protein At4g21065-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0022426
Physical Location & Seq
Forward (+)
6346140 .. 6347918
1779 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1398 bp
ATGGTGGATGATGGGGTTGAGGTGAATGATGCAACGGTGGTGTCAGTTTTGAGAGCGTGTGCGGAGACAGGGGCGTTGGGCGTAGGGAGGAGAGTGCATGGGGTTGCTGAGGATAGTGGGATTGGTTCGAAGCCTAATGTTAGTACTGCCCTTATTGATATGTTTGCAAAATGTGGATGTATGCAGAGTGCAAGGCGGGTTTTCAATGACGTTGTGGATAAAGACGTTTTTGCATGGACGGCTATGATATCCGGACTTGCAAGCCATGGCCATTGTCAAGATGCTATTGAATTGTTCAGTAAAATGCAAGATTTTGGTGTGAAACCTGATGGGAGGACTATGACTGCAGTTTTATCGGCATGTAGGAATGCAGGTCGGGTTGAGGAAGGTTACGCGTACATGACAAGTATGCAGAAGGATTATGGAGTTAGGCCTACAATTCAACACTATGGATGCATGGTAGACCTCCTTGCTAGAGCGGGTCATTTGAAAGATGCTGAAGAGTTCATCAGGAAAATGCCCATGGAACCTGATGCAGTTTTGTTCAGAAACATGATATGGGCATGCAAAGTGCATAACGATACCAATCGAGCGGAACGCTTGATAAAATATCTTCAACAGCTAAAGACGAACTCAAATGATAGCGGAAGTTTTGTACTTATTGGCAATGTGTATGCATCAGCAGGCAAATGGCACGACAAAGCAAAGGTTAGAGATTTGATGAAGCAAAAAGGACTAGTAAAGCCACCAGGGTTTAGTAGGATTGAGGTTGATGGGGAAATCCACGAATTTGCTGTCGGAGATTCAGGACACCCTGAAGCCGAAACAATTTACAGAAAGTTGGAAGAAATAGAAGATTATTTGAGAAAAGAAGGGTACAGTCCCAAACTTTCAGAAGTTTTGCTTGAAATCGACGATGAGGAGAAAGCTTTTCAATTGAGTCACCACAGTGAGAAGCTTGCTCTTGCCTTTGGTCTGATTAGAACACGTCCAGGAGGAGAAACTATACCCTATCTCTTGGAACAACTGTTGTATCTGAATTTCCTCCTCAATGGACCGTTTAAGGTTTCGAATATGGCCCTCAAAGCATCAGAAACCCTAATCTCTTCAGCCCTCATCTCAACAACCTCTTTTGTCAACCCATCAAGAACCCTGAGTAGCTCGGCACACTCAAAGCGCTTATGCAAGAACATCAGCAGTAATCCCAGCTCCTCCAAATCCAAATTCAACACGCATATCGGCAGTTTAGTCCGAATAAGTGGTGGTGATGATACCGACATGGCATTCTCAGATTCTTGCTGCGCAAGAAAGAGACCAGATCCTCCTTGTGCCTACATTTTTTTAACAGCTTGTCATGCTCAGCCAATTTCACTTATTTTTGGCCTCATTACCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

466

Amino Acids

51.16

Weight (kDa)

6.92

Isoelectric Point (pI)

41.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 74 - 121 1.9e-11 PPR repeat family
PPR PF01535 78 - 107 2.2e-07 PPR repeat
E_motif PF20431 192 - 256 7.6e-15 E motif
DYW_deaminase PF14432 292 - 332 1.1e-07 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022426)

Species Orthologous Gene IDs
malus_domestica MD05G1062000.v1.1
prunus_persica Prupe.8G091500_v2.0.a1 Prupe.8G091500_v2.0.a1
pyrus_communis pycom05g05340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1303
Acc36I ACCTGC 1 cut(s) 362
AccBSI CCGCTC 2 cut(s) 479, 593
AccI GTMKAC 1 cut(s) 462
AccII CGCG 1 cut(s) 395
AccIII TCCGGA 1 cut(s) 251
AciI CCGC 5 cut(s) 62, 196, 479, 593, 645
AclWI GGATC 1 cut(s) 1313
AcoI YGGCCR 1 cut(s) 268
AcsI RAATTY 3 cut(s) 788, 1039, 1223
AcuI CTGAAG 3 cut(s) 519, 837, 1092
AfaI GTAC 4 cut(s) 145, 398, 657, 878
AfeI AGCGCT 1 cut(s) 1178
AflIII ACRYGT 2 cut(s) 393, 986
AgsI TTSAA 8 cut(s) 205, 290, 443, 490, 617, 908, 935, 1228
AhlI ACTAGT 1 cut(s) 736
AjiI CACGTC 1 cut(s) 989
AjnI CCWGG 2 cut(s) 748, 991
AjuI GAANNNNNNNTTGG 2 cut(s) 1208, 1240
AleI CACNNNNGTG 1 cut(s) 948
AluBI AGCT 6 cut(s) 622, 929, 958, 1161, 1209, 1349
AluI AGCT 6 cut(s) 622, 929, 958, 1161, 1209, 1349
Alw26I GTCTC 2 cut(s) 59, 1306
AlwI GGATC 1 cut(s) 1313
Aor13HI TCCGGA 1 cut(s) 251
Aor51HI AGCGCT 1 cut(s) 1178
AoxI GGCC 4 cut(s) 268, 431, 1077, 1381
ApeKI GCWGC 1 cut(s) 1299
ApoI RAATTY 3 cut(s) 788, 1039, 1223
ArsI GACNNNNNNTTYG 2 cut(s) 780, 812
AspLEI GCGC 2 cut(s) 1179, 1304
AspS9I GGNCC 2 cut(s) 1055, 1078
AsuHPI GGTGA 3 cut(s) 34, 935, 1277
AsuII TTCGAA 2 cut(s) 128, 1070
AvaII GGWCC 1 cut(s) 1055
BaeI ACNNNNGTAYC 2 cut(s) 1016, 1049
BalI TGGCCA 1 cut(s) 270
BbvCI CCTCAGC 1 cut(s) 108
BbvI GCAGC 1 cut(s) 1286
BccI CCATC 4 cut(s) 5, 323, 767, 1150
BceAI ACGGC 1 cut(s) 255
BcgI CGANNNNNNTGC 2 cut(s) 336, 370
BciT130I CCWGG 2 cut(s) 750, 993
BcoDI GTCTC 2 cut(s) 59, 1306
BcuI ACTAGT 1 cut(s) 736
BfaI CTAG 2 cut(s) 474, 737
BfmI CTRYAG 1 cut(s) 345
BfoI RGCGCY 1 cut(s) 1180
BfuAI ACCTGC 1 cut(s) 362
BisI GCNGC 1 cut(s) 1300
BlpI GCTNAGC 1 cut(s) 1359
BlsI GCNGC 1 cut(s) 1301
BmcAI AGTACT 1 cut(s) 145
Bme1390I CCNGG 2 cut(s) 750, 993
Bme18I GGWCC 1 cut(s) 1055
BmgBI CACGTC 1 cut(s) 989
BmgT120I GGNCC 2 cut(s) 1055, 1078
BmiI GGNNCC 1 cut(s) 528
BmrFI CCNGG 2 cut(s) 750, 993
BmsI GCATC 7 cut(s) 19, 271, 443, 484, 523, 686, 1097
Bpu10I CCTNAGC 1 cut(s) 108
Bpu1102I GCTNAGC 1 cut(s) 1359
Bpu14I TTCGAA 2 cut(s) 128, 1070
BsaBI GATNNNNATC 1 cut(s) 585
BsaI GGTCTC 1 cut(s) 1306
BsaJI CCNNGG 3 cut(s) 265, 522, 749
BsaWI WCCGGW 1 cut(s) 251
Bse3DI GCAATG 1 cut(s) 673
Bse8I GATNNNNATC 1 cut(s) 585
BseAI TCCGGA 1 cut(s) 251
BseBI CCWGG 2 cut(s) 750, 993
BseDI CCNNGG 3 cut(s) 265, 522, 749
BseGI GGATG 3 cut(s) 13, 182, 458
BseJI GATNNNNATC 1 cut(s) 585
BseMI GCAATG 1 cut(s) 673
BseMII CTCAG 4 cut(s) 99, 1145, 1302, 1373
BseRI GAGGAG 5 cut(s) 103, 935, 1011, 1037, 1201
BseXI GCAGC 1 cut(s) 1286
BseYI CCCAGC 1 cut(s) 1205
Bsh1236I CGCG 1 cut(s) 395
BshFI GGCC 4 cut(s) 270, 433, 1079, 1383
BsiSI CCGG 1 cut(s) 252
BslFI GGGAC 1 cut(s) 867
BsmAI GTCTC 2 cut(s) 59, 1306
BsmFI GGGAC 1 cut(s) 867
BsmI GAATGC 2 cut(s) 373, 1283
BsnI GGCC 4 cut(s) 270, 433, 1079, 1383
Bso31I GGTCTC 1 cut(s) 1306
Bsp119I TTCGAA 2 cut(s) 128, 1070
Bsp13I TCCGGA 1 cut(s) 251
Bsp143I GATC 1 cut(s) 1318
Bsp1720I GCTNAGC 1 cut(s) 1359
Bsp19I CCATGG 2 cut(s) 265, 522
BspACI CCGC 5 cut(s) 62, 196, 479, 593, 645
BspANI GGCC 4 cut(s) 270, 433, 1079, 1383
BspCNI CTCAG 4 cut(s) 100, 1146, 1301, 1372
BspEI TCCGGA 1 cut(s) 251
BspFNI CGCG 1 cut(s) 395
BspLI GGNNCC 1 cut(s) 528
BspMAI CTGCAG 1 cut(s) 349
BspMI ACCTGC 1 cut(s) 362
BspPI GGATC 1 cut(s) 1313
BspT104I TTCGAA 2 cut(s) 128, 1070
BspTNI GGTCTC 1 cut(s) 1306
BsrBI CCGCTC 2 cut(s) 479, 593
BsrDI GCAATG 1 cut(s) 673
BssECI CCNNGG 3 cut(s) 265, 522, 749
BssMI GATC 1 cut(s) 1318
BssT1I CCWWGG 2 cut(s) 265, 522
Bst2UI CCWGG 2 cut(s) 750, 993
Bst4CI ACNGT 5 cut(s) 37, 881, 950, 1029, 1059
Bst6I CTCTTC 2 cut(s) 495, 1111
BstBI TTCGAA 2 cut(s) 128, 1070
BstC8I GCNNGC 4 cut(s) 262, 565, 685, 960
BstDEI CTNAG 4 cut(s) 108, 1154, 1288, 1359
BstDSI CCRYGG 2 cut(s) 265, 522
BstF5I GGATG 3 cut(s) 13, 182, 458
BstFNI CGCG 1 cut(s) 395
BstH2I RGCGCY 1 cut(s) 1180
BstHHI GCGC 2 cut(s) 1179, 1304
BstKTI GATC 1 cut(s) 1321
BstMAI GTCTC 2 cut(s) 59, 1306
BstMBI GATC 1 cut(s) 1318
BstMWI GCNNNNNNNGC 3 cut(s) 239, 1085, 1355
BstNI CCWGG 2 cut(s) 750, 993
BstNSI RCATGY 2 cut(s) 363, 567
BstSCI CCNGG 2 cut(s) 748, 991
BstSFI CTRYAG 1 cut(s) 345
BstUI CGCG 1 cut(s) 395
BstV1I GCAGC 1 cut(s) 1286
BstX2I RGATCY 1 cut(s) 1318
BstYI RGATCY 1 cut(s) 1318
BsuRI GGCC 4 cut(s) 270, 433, 1079, 1383
BtgI CCRYGG 2 cut(s) 265, 522
BtrI CACGTC 1 cut(s) 989
BtsCI GGATG 3 cut(s) 13, 182, 458
BtsIMutI CAGTG 1 cut(s) 955
BveI ACCTGC 1 cut(s) 362
Cac8I GCNNGC 4 cut(s) 262, 565, 685, 960
CfoI GCGC 2 cut(s) 1179, 1304
Cfr13I GGNCC 2 cut(s) 1055, 1078
Csp6I GTAC 4 cut(s) 144, 397, 656, 877
CviQI GTAC 4 cut(s) 144, 397, 656, 877
DdeI CTNAG 4 cut(s) 108, 1154, 1288, 1359
DpnI GATC 1 cut(s) 1320
DpnII GATC 1 cut(s) 1318
EaeI YGGCCR 1 cut(s) 268
Eam1104I CTCTTC 2 cut(s) 495, 1111
EarI CTCTTC 2 cut(s) 495, 1111
Eco130I CCWWGG 2 cut(s) 265, 522
Eco147I AGGCCT 1 cut(s) 433
Eco31I GGTCTC 1 cut(s) 1306
Eco32I GATATC 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 1055
Eco47III AGCGCT 1 cut(s) 1178
Eco57I CTGAAG 3 cut(s) 519, 837, 1092
EcoRII CCWGG 2 cut(s) 748, 991
EcoRV GATATC 1 cut(s) 249
EcoT14I CCWWGG 2 cut(s) 265, 522
EcoT22I ATGCAT 2 cut(s) 458, 679
ErhI CCWWGG 2 cut(s) 265, 522
FalI AAGNNNNNCTT 2 cut(s) 888, 920
FaqI GGGAC 1 cut(s) 867
FauI CCCGC 2 cut(s) 189, 472
FblI GTMKAC 1 cut(s) 462
Fnu4HI GCNGC 1 cut(s) 1300
FokI GGATG 3 cut(s) 20, 189, 465
Fsp4HI GCNGC 1 cut(s) 1300
FspBI CTAG 2 cut(s) 474, 737
FspI TGCGCA 1 cut(s) 1303
GlaI GCGC 2 cut(s) 1178, 1303
GluI GCNGC 1 cut(s) 1300
GsaI CCCAGC 1 cut(s) 1209
HaeII RGCGCY 1 cut(s) 1180
HaeIII GGCC 4 cut(s) 270, 433, 1079, 1383
HapII CCGG 1 cut(s) 252
HhaI GCGC 2 cut(s) 1179, 1304
Hin6I GCGC 2 cut(s) 1177, 1302
HinP1I GCGC 2 cut(s) 1177, 1302
HincII GTYRAC 1 cut(s) 1138
HindII GTYRAC 1 cut(s) 1138
HindIII AAGCTT 2 cut(s) 927, 956
HinfI GANTC 3 cut(s) 803, 940, 1292
HpaII CCGG 1 cut(s) 252
HphI GGTGA 3 cut(s) 34, 935, 1277
Hpy166II GTNNAC 2 cut(s) 463, 1138
Hpy188I TCNGA 8 cut(s) 548, 800, 895, 978, 1038, 1093, 1253, 1291
Hpy188III TCNNGA 5 cut(s) 252, 278, 511, 807, 1146
Hpy8I GTNNAC 2 cut(s) 463, 1138
Hpy99I CGWCG 1 cut(s) 917
HpyAV CCTTC 3 cut(s) 380, 409, 866
HpyCH4III ACNGT 5 cut(s) 37, 881, 950, 1029, 1059
HpyCH4IV ACGT 3 cut(s) 210, 225, 988
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 1085, 1355
HpyF3I CTNAG 4 cut(s) 108, 1154, 1288, 1359
HpySE526I ACGT 3 cut(s) 210, 225, 988
HspAI GCGC 2 cut(s) 1177, 1302
Kpn2I TCCGGA 1 cut(s) 251
Kzo9I GATC 1 cut(s) 1318
LmnI GCTCC 1 cut(s) 1214
Lsp1109I GCAGC 1 cut(s) 1286
LweI GCATC 7 cut(s) 19, 271, 443, 484, 523, 686, 1097
MaeI CTAG 2 cut(s) 474, 737
MaeII ACGT 3 cut(s) 210, 225, 988
MaeIII GTNAC 2 cut(s) 389, 941
MalI GATC 1 cut(s) 1320
MbiI CCGCTC 2 cut(s) 479, 593
MboI GATC 1 cut(s) 1318
MboII GAAGA 5 cut(s) 512, 605, 857, 866, 1098
MfeI CAATTG 1 cut(s) 935
MflI RGATCY 1 cut(s) 1318
MlsI TGGCCA 1 cut(s) 270
MluCI AATT 8 cut(s) 290, 438, 788, 828, 935, 1039, 1223, 1365
MluI ACGCGT 1 cut(s) 393
MluNI TGGCCA 1 cut(s) 270
MlyI GAGTC 1 cut(s) 949
MmeI TCCRAC 2 cut(s) 778, 822
Mox20I TGGCCA 1 cut(s) 270
Mph1103I ATGCAT 2 cut(s) 458, 679
MroI TCCGGA 1 cut(s) 251
MscI TGGCCA 1 cut(s) 270
MseI TTAA 2 cut(s) 1062, 1343
MslI CAYNNNNRTG 1 cut(s) 948
Msp20I TGGCCA 1 cut(s) 270
MspI CCGG 1 cut(s) 252
MspR9I CCNGG 2 cut(s) 750, 993
MunI CAATTG 1 cut(s) 935
Mva1269I GAATGC 2 cut(s) 373, 1283
MvaI CCWGG 2 cut(s) 750, 993
MvnI CGCG 1 cut(s) 395
MwoI GCNNNNNNNGC 3 cut(s) 239, 1085, 1355
NcoI CCATGG 2 cut(s) 265, 522
NdeII GATC 1 cut(s) 1318
NlaIV GGNNCC 1 cut(s) 528
NmeAIII GCCGAG 1 cut(s) 1142
NmuCI GTSAC 1 cut(s) 941
NsbI TGCGCA 1 cut(s) 1303
NsiI ATGCAT 2 cut(s) 458, 679
NspI RCATGY 2 cut(s) 363, 567
NspV TTCGAA 2 cut(s) 128, 1070
OliI CACNNNNGTG 1 cut(s) 948
PaeI GCATGC 1 cut(s) 567
PceI AGGCCT 1 cut(s) 433
PctI GAATGC 2 cut(s) 373, 1283
PfeI GAWTC 2 cut(s) 803, 1292
PfoI TCCNGGA 1 cut(s) 991
PkrI GCNGC 1 cut(s) 1301
PleI GAGTC 1 cut(s) 948
PpsI GAGTC 1 cut(s) 948
Psp6I CCWGG 2 cut(s) 748, 991
PspFI CCCAGC 1 cut(s) 1205
PspGI CCWGG 2 cut(s) 748, 991
PspN4I GGNNCC 1 cut(s) 528
PspPI GGNCC 2 cut(s) 1055, 1078
PstI CTGCAG 1 cut(s) 349
PsuI RGATCY 1 cut(s) 1318
RsaI GTAC 4 cut(s) 145, 398, 657, 878
RsaNI GTAC 4 cut(s) 144, 397, 656, 877
RseI CAYNNNNRTG 1 cut(s) 948
SaqAI TTAA 2 cut(s) 1062, 1343
SatI GCNGC 1 cut(s) 1300
Sau3AI GATC 1 cut(s) 1318
Sau96I GGNCC 2 cut(s) 1055, 1078
ScaI AGTACT 1 cut(s) 145
SchI GAGTC 1 cut(s) 949
ScrFI CCNGG 2 cut(s) 750, 993
SfaNI GCATC 7 cut(s) 19, 271, 443, 484, 523, 686, 1097
SfcI CTRYAG 1 cut(s) 345
SfuI TTCGAA 2 cut(s) 128, 1070
SinI GGWCC 1 cut(s) 1055
SmiMI CAYNNNNRTG 1 cut(s) 948
SpeI ACTAGT 1 cut(s) 736
SphI GCATGC 1 cut(s) 567
Sse9I AATT 8 cut(s) 290, 438, 788, 828, 935, 1039, 1223, 1365
SseBI AGGCCT 1 cut(s) 433
SsiI CCGC 5 cut(s) 62, 196, 479, 593, 645
SspMI CTAG 2 cut(s) 474, 737
StuI AGGCCT 1 cut(s) 433
StyD4I CCNGG 2 cut(s) 748, 991
StyI CCWWGG 2 cut(s) 265, 522
TaaI ACNGT 5 cut(s) 37, 881, 950, 1029, 1059
TaiI ACGT 3 cut(s) 213, 228, 991
TaqI TCGA 4 cut(s) 128, 589, 912, 1070
TasI AATT 8 cut(s) 290, 438, 788, 828, 935, 1039, 1223, 1365
TatI WGTACW 2 cut(s) 143, 655
TfiI GAWTC 2 cut(s) 803, 1292
Tru1I TTAA 2 cut(s) 1062, 1343
Tru9I TTAA 2 cut(s) 1062, 1343
TscAI CASTG 1 cut(s) 955
TseFI GTSAC 1 cut(s) 941
TseI GCWGC 1 cut(s) 1299
Tsp45I GTSAC 1 cut(s) 941
TspDTI ATGAA 2 cut(s) 496, 737
TspRI CASTG 1 cut(s) 955
VpaK11BI GGWCC 1 cut(s) 1055
XapI RAATTY 3 cut(s) 788, 1039, 1223
XceI RCATGY 2 cut(s) 363, 567
XmiI GTMKAC 1 cut(s) 462
XspI CTAG 2 cut(s) 474, 737
ZrmI AGTACT 1 cut(s) 145
Zsp2I ATGCAT 2 cut(s) 458, 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.