pycom05g08420

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0029269
Physical Location & Seq
Forward (+)
11027425 .. 11028646
1222 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 930 bp
ATGACGTTACTTGTCTTCAACTCTGCCTTAATAGTCGTACCAACCTCACTTGGAAGGGCAATTTTCAATGTCATTCCTTTTCTCCCTATAACACATGGAATCAAGTGCAATGATTTGTATGCTTTCATCATTGGAAGCTACATAATATGGACTGCTGTGGCCGGAGTAAGATACTCAATTGAGCATATTCGAACAAAGAGGGTTGCAGTTCTGTTAGGCCAGATCTGGAAGTGGTGTGCCATTGTTATCAAGAGTTCCGCACTTTTGTCAATATGGATTTTTGTCATTCCGGTATTGATTGGGCTGCTCTTTGAGCTTCTGGTGATCGTGCCAATGCGTGTGCCTGTTGATGAAAGCCCAGTGTTCTTGCTTTACCAGGACTGGGCATTGGGCCTTATTTTTCTCAAGATATGGACTAGACTGGTTATGTTGGATCACATGATGCCACTGGTGGATGAAACGTGGCGAGTAAAGTTCGAAAGGGTGAGGGAAGACGGTTTCTCTAGGCTGCAAGGGCTTTGGGTGCTTCGCGAGATTGTATTCCCAATCATAATGAAGTTGTTGACTGCCCTGTGTGTGCCTTACGTACTTGCCAGAGGGCTATTTCCTGTTCTCGGTTACCCATTAGTGGTGAATTCAGCAGTTTATCGGTTTGCCTGGCTGGGATGCCTTTGCTTTAGCCTGTTGTGCTTTTGCGCCAAGAGATTCCATGTCTGGTTCACCAACCTTCACAACTCAATACGTGACGACCGGTATCTAGTTGGTCGTAGGCTTCATAACTTTGGAGAAGCCGTCGAAGAGAAGCAAAACGAAGCAGGGACATCCTCGGAAGTGCAGGGCTCTAACTTGGAGACCAACGGGCTAATCCGGTACGACCGAGAAGTTGATATAGGACTCCGGCTAAGGCACGTTAACCGAGTTGATGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000209 GO:0001101 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006508 GO:0006511 GO:0006629 GO:0006720 GO:0006725 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009414 GO:0009415 GO:0009628 GO:0009698 GO:0009699 GO:0009893 GO:0009987 GO:0010025 GO:0010033 GO:0010035 GO:0010143 GO:0010166 GO:0010243 GO:0010345 GO:0010498 GO:0012505 GO:0016020 GO:0016021 GO:0016567 GO:0016740 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0030176 GO:0030433 GO:0031224 GO:0031227 GO:0031624 GO:0031984 GO:0032446 GO:0032501 GO:0032502 GO:0033554 GO:0034976 GO:0036211 GO:0036503 GO:0042175 GO:0042221 GO:0042335 GO:0043085 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0048518 GO:0048856 GO:0050789 GO:0050790 GO:0050896 GO:0051341 GO:0051353 GO:0051603 GO:0051716 GO:0065007 GO:0065009 GO:0070647 GO:0070936 GO:0071704 GO:0098827 GO:0140096 GO:1900490 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901575 GO:1901576 GO:1901698 GO:1901700 GO:1990381 GO:2001215
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
RINGv

Protein Analysis

310

Amino Acids

35.5

Weight (kDa)

9.11

Isoelectric Point (pI)

28.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MARCHF6_C PF23113 70 - 248 1e-51 E3 ubiquitin-protein ligase MARCHF6-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0029269)

Species Orthologous Gene IDs
pyrus_communis pycom05g08420
rosa_multiflora Rmu_sc0024606.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 531
AciI CCGC 1 cut(s) 258
AclWI GGATC 1 cut(s) 441
AcoI YGGCCR 1 cut(s) 159
AcsI RAATTY 1 cut(s) 634
AfaI GTAC 3 cut(s) 39, 588, 872
AfiI CCNNNNNNNGG 3 cut(s) 382, 614, 628
AgeI ACCGGT 1 cut(s) 750
AgsI TTSAA 2 cut(s) 19, 67
AjnI CCWGG 2 cut(s) 375, 656
AluBI AGCT 2 cut(s) 138, 316
AluI AGCT 2 cut(s) 138, 316
Alw26I GTCTC 1 cut(s) 845
AlwI GGATC 1 cut(s) 441
AoxI GGCC 3 cut(s) 159, 217, 391
ApeKI GCWGC 2 cut(s) 304, 508
ApoI RAATTY 1 cut(s) 634
AsiGI ACCGGT 1 cut(s) 750
AspLEI GCGC 1 cut(s) 698
AspS9I GGNCC 1 cut(s) 391
AsuHPI GGTGA 4 cut(s) 334, 496, 643, 712
AsuII TTCGAA 2 cut(s) 190, 477
BanII GRGCYC 1 cut(s) 842
BbsI GAAGAC 2 cut(s) 7, 498
BbvI GCAGC 2 cut(s) 291, 495
BceAI ACGGC 1 cut(s) 776
BciT130I CCWGG 2 cut(s) 377, 658
BcoDI GTCTC 1 cut(s) 845
BfaI CTAG 3 cut(s) 417, 504, 758
BglII AGATCT 1 cut(s) 222
BisI GCNGC 2 cut(s) 305, 509
BlsI GCNGC 2 cut(s) 306, 510
Bme1390I CCNGG 2 cut(s) 377, 658
BmgT120I GGNCC 1 cut(s) 391
BmrFI CCNGG 2 cut(s) 377, 658
BmrI ACTGGG 2 cut(s) 353, 391
BmsI GCATC 3 cut(s) 432, 656, 913
BmuI ACTGGG 2 cut(s) 353, 391
BpiI GAAGAC 2 cut(s) 7, 498
Bpu10I CCTNAGC 1 cut(s) 902
Bpu14I TTCGAA 2 cut(s) 190, 477
BpuEI CTTGAG 1 cut(s) 389
BsaAI YACGTR 2 cut(s) 586, 743
BsaI GGTCTC 1 cut(s) 845
BsaJI CCNNGG 1 cut(s) 825
BsaWI WCCGGW 3 cut(s) 289, 750, 867
BsaXI ACNNNNNCTCC 2 cut(s) 777, 807
Bsc4I CCNNNNNNNGG 3 cut(s) 382, 614, 628
Bse118I RCCGGY 1 cut(s) 750
Bse1I ACTGG 4 cut(s) 359, 386, 426, 453
Bse3DI GCAATG 1 cut(s) 115
BseBI CCWGG 2 cut(s) 377, 658
BseDI CCNNGG 1 cut(s) 825
BseGI GGATG 3 cut(s) 460, 671, 821
BseLI CCNNNNNNNGG 3 cut(s) 382, 614, 628
BseMI GCAATG 1 cut(s) 115
BseNI ACTGG 4 cut(s) 359, 386, 426, 453
BseXI GCAGC 2 cut(s) 291, 495
BseYI CCCAGC 1 cut(s) 661
BsgI GTGCAG 1 cut(s) 854
Bsh1236I CGCG 1 cut(s) 531
Bsh1285I CGRYCG 2 cut(s) 751, 877
BshFI GGCC 3 cut(s) 161, 219, 393
BshTI ACCGGT 1 cut(s) 750
BsiEI CGRYCG 2 cut(s) 751, 877
BsiSI CCGG 5 cut(s) 162, 290, 751, 868, 898
BslFI GGGAC 1 cut(s) 832
BslI CCNNNNNNNGG 3 cut(s) 382, 614, 628
BsmAI GTCTC 1 cut(s) 845
BsmFI GGGAC 1 cut(s) 832
BsnI GGCC 3 cut(s) 161, 219, 393
Bso31I GGTCTC 1 cut(s) 845
Bsp119I TTCGAA 2 cut(s) 190, 477
Bsp1286I GDGCHC 1 cut(s) 842
Bsp143I GATC 3 cut(s) 222, 324, 433
Bsp68I TCGCGA 1 cut(s) 531
BspACI CCGC 1 cut(s) 258
BspANI GGCC 3 cut(s) 161, 219, 393
BspFNI CGCG 1 cut(s) 531
BspPI GGATC 1 cut(s) 441
BspT104I TTCGAA 2 cut(s) 190, 477
BspTNI GGTCTC 1 cut(s) 845
BsrDI GCAATG 1 cut(s) 115
BsrFI RCCGGY 1 cut(s) 750
BsrI ACTGG 4 cut(s) 359, 386, 426, 453
BssAI RCCGGY 1 cut(s) 750
BssECI CCNNGG 1 cut(s) 825
BssMI GATC 3 cut(s) 222, 324, 433
Bst2UI CCWGG 2 cut(s) 377, 658
Bst4CI ACNGT 1 cut(s) 497
Bst6I CTCTTC 1 cut(s) 792
BstBAI YACGTR 2 cut(s) 586, 743
BstBI TTCGAA 2 cut(s) 190, 477
BstDEI CTNAG 1 cut(s) 902
BstEII GGTNACC 1 cut(s) 617
BstF5I GGATG 3 cut(s) 460, 671, 821
BstFNI CGCG 1 cut(s) 531
BstHHI GCGC 1 cut(s) 698
BstKTI GATC 3 cut(s) 225, 327, 436
BstMAI GTCTC 1 cut(s) 845
BstMBI GATC 3 cut(s) 222, 324, 433
BstMCI CGRYCG 2 cut(s) 751, 877
BstMWI GCNNNNNNNGC 4 cut(s) 313, 514, 523, 687
BstNI CCWGG 2 cut(s) 377, 658
BstPI GGTNACC 1 cut(s) 617
BstSCI CCNGG 2 cut(s) 375, 656
BstSNI TACGTA 1 cut(s) 586
BstUI CGCG 1 cut(s) 531
BstV1I GCAGC 2 cut(s) 291, 495
BstV2I GAAGAC 2 cut(s) 7, 498
BstX2I RGATCY 1 cut(s) 222
BstYI RGATCY 1 cut(s) 222
BsuRI GGCC 3 cut(s) 161, 219, 393
BtsCI GGATG 3 cut(s) 460, 671, 821
BtsIMutI CAGTG 2 cut(s) 366, 446
BtuMI TCGCGA 1 cut(s) 531
CfoI GCGC 1 cut(s) 698
Cfr10I RCCGGY 1 cut(s) 750
Cfr13I GGNCC 1 cut(s) 391
Csp6I GTAC 3 cut(s) 38, 587, 871
CspAI ACCGGT 1 cut(s) 750
CviAII CATG 3 cut(s) 95, 439, 710
CviQI GTAC 3 cut(s) 38, 587, 871
DdeI CTNAG 1 cut(s) 902
DpnI GATC 3 cut(s) 224, 326, 435
DpnII GATC 3 cut(s) 222, 324, 433
EaeI YGGCCR 1 cut(s) 159
Eam1104I CTCTTC 1 cut(s) 792
EarI CTCTTC 1 cut(s) 792
Eco105I TACGTA 1 cut(s) 586
Eco24I GRGCYC 1 cut(s) 842
Eco31I GGTCTC 1 cut(s) 845
Eco91I GGTNACC 1 cut(s) 617
EcoO65I GGTNACC 1 cut(s) 617
EcoRI GAATTC 1 cut(s) 634
EcoRII CCWGG 2 cut(s) 375, 656
EcoT38I GRGCYC 1 cut(s) 842
FaeI CATG 3 cut(s) 98, 442, 713
FaqI GGGAC 1 cut(s) 832
FatI CATG 3 cut(s) 94, 438, 709
Fnu4HI GCNGC 2 cut(s) 305, 509
FokI GGATG 3 cut(s) 467, 678, 808
FriOI GRGCYC 1 cut(s) 842
Fsp4HI GCNGC 2 cut(s) 305, 509
FspBI CTAG 3 cut(s) 417, 504, 758
GlaI GCGC 1 cut(s) 697
GluI GCNGC 2 cut(s) 305, 509
GsaI CCCAGC 1 cut(s) 665
HaeIII GGCC 3 cut(s) 161, 219, 393
HapII CCGG 5 cut(s) 162, 290, 751, 868, 898
HhaI GCGC 1 cut(s) 698
Hin1II CATG 3 cut(s) 98, 442, 713
Hin6I GCGC 1 cut(s) 696
HinP1I GCGC 1 cut(s) 696
HincII GTYRAC 2 cut(s) 564, 913
HindII GTYRAC 2 cut(s) 564, 913
HinfI GANTC 3 cut(s) 99, 705, 894
HpaI GTTAAC 1 cut(s) 913
HpaII CCGG 5 cut(s) 162, 290, 751, 868, 898
HphI GGTGA 4 cut(s) 334, 496, 643, 712
Hpy166II GTNNAC 3 cut(s) 564, 720, 913
Hpy188I TCNGA 1 cut(s) 829
Hpy188III TCNNGA 4 cut(s) 226, 250, 406, 530
Hpy8I GTNNAC 3 cut(s) 564, 720, 913
Hpy99I CGWCG 1 cut(s) 797
HpyAV CCTTC 2 cut(s) 48, 737
HpyCH4III ACNGT 1 cut(s) 497
HpyCH4IV ACGT 5 cut(s) 5, 461, 585, 742, 909
HpyCH4V TGCA 4 cut(s) 108, 206, 511, 835
HpyF10VI GCNNNNNNNGC 4 cut(s) 313, 514, 523, 687
HpyF3I CTNAG 1 cut(s) 902
HpySE526I ACGT 5 cut(s) 5, 461, 585, 742, 909
Hsp92II CATG 3 cut(s) 98, 442, 713
HspAI GCGC 1 cut(s) 696
KspAI GTTAAC 1 cut(s) 913
Kzo9I GATC 3 cut(s) 222, 324, 433
Lsp1109I GCAGC 2 cut(s) 291, 495
LweI GCATC 3 cut(s) 432, 656, 913
MaeI CTAG 3 cut(s) 417, 504, 758
MaeII ACGT 5 cut(s) 5, 461, 585, 742, 909
MaeIII GTNAC 3 cut(s) 6, 617, 743
MalI GATC 3 cut(s) 224, 326, 435
MboI GATC 3 cut(s) 222, 324, 433
MboII GAAGA 3 cut(s) 7, 503, 809
MfeI CAATTG 1 cut(s) 177
MflI RGATCY 1 cut(s) 222
MhlI GDGCHC 1 cut(s) 842
MluCI AATT 3 cut(s) 60, 177, 634
MlyI GAGTC 1 cut(s) 888
MmeI TCCRAC 1 cut(s) 411
MnlI CCTC 5 cut(s) 55, 192, 480, 590, 835
MseI TTAA 2 cut(s) 29, 912
MspI CCGG 5 cut(s) 162, 290, 751, 868, 898
MspR9I CCNGG 2 cut(s) 377, 658
MunI CAATTG 1 cut(s) 177
MvaI CCWGG 2 cut(s) 377, 658
MvnI CGCG 1 cut(s) 531
MwoI GCNNNNNNNGC 4 cut(s) 313, 514, 523, 687
NdeII GATC 3 cut(s) 222, 324, 433
NlaIII CATG 3 cut(s) 98, 442, 713
NmuCI GTSAC 1 cut(s) 743
NruI TCGCGA 1 cut(s) 531
NspV TTCGAA 2 cut(s) 190, 477
PfeI GAWTC 2 cut(s) 99, 705
PinAI ACCGGT 1 cut(s) 750
PkrI GCNGC 2 cut(s) 306, 510
PleI GAGTC 1 cut(s) 888
PpsI GAGTC 1 cut(s) 888
Ppu21I YACGTR 2 cut(s) 586, 743
Psp6I CCWGG 2 cut(s) 375, 656
PspEI GGTNACC 1 cut(s) 617
PspFI CCCAGC 1 cut(s) 661
PspGI CCWGG 2 cut(s) 375, 656
PspPI GGNCC 1 cut(s) 391
PsuI RGATCY 1 cut(s) 222
RruI TCGCGA 1 cut(s) 531
RsaI GTAC 3 cut(s) 39, 588, 872
RsaNI GTAC 3 cut(s) 38, 587, 871
SaqAI TTAA 2 cut(s) 29, 912
SatI GCNGC 2 cut(s) 305, 509
Sau3AI GATC 3 cut(s) 222, 324, 433
Sau96I GGNCC 1 cut(s) 391
SchI GAGTC 1 cut(s) 888
ScrFI CCNGG 2 cut(s) 377, 658
SduI GDGCHC 1 cut(s) 842
SetI ASST 9 cut(s) 8, 47, 140, 318, 464, 588, 729, 745, 912
SfaNI GCATC 3 cut(s) 432, 656, 913
SfuI TTCGAA 2 cut(s) 190, 477
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
SnaBI TACGTA 1 cut(s) 586
Sse9I AATT 3 cut(s) 60, 177, 634
SsiI CCGC 1 cut(s) 258
SspMI CTAG 3 cut(s) 417, 504, 758
StyD4I CCNGG 2 cut(s) 375, 656
TaaI ACNGT 1 cut(s) 497
TaiI ACGT 5 cut(s) 8, 464, 588, 745, 912
TaqI TCGA 3 cut(s) 190, 477, 795
TaqII GACCGA 1 cut(s) 891
TasI AATT 3 cut(s) 60, 177, 634
TfiI GAWTC 2 cut(s) 99, 705
Tru1I TTAA 2 cut(s) 29, 912
Tru9I TTAA 2 cut(s) 29, 912
TscAI CASTG 2 cut(s) 366, 453
TseFI GTSAC 1 cut(s) 743
TseI GCWGC 2 cut(s) 304, 508
Tsp45I GTSAC 1 cut(s) 743
TspDTI ATGAA 5 cut(s) 115, 366, 471, 569, 764
TspRI CASTG 2 cut(s) 366, 453
XapI RAATTY 1 cut(s) 634
XspI CTAG 3 cut(s) 417, 504, 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.