pycom05g09200

Indole-3-acetic acid-amido synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0029270
Physical Location & Seq
Forward (+)
12307938 .. 12308398
461 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 351 bp
ATGGCTGTCGACTCCTCACTCTCTCCCCTCGGCCCACCGGCCTGCGACAAGGACGCCAAAGCGTTACAGTTCATTGAAGAAACTACCCGGAACGCCGACGCCGTGCAAGAGCGGGTGCTGTCCGAGATACTAGCTCGAAACGCCCAGACAGAGTACCTTAAACGCTTCAAACTCGGCGGCGCCACCGACCGCAAAACTTTCAAGTCCAAACTCCCCGTGATTACCTACGAGGATCGATCCAGCCCGAAATCCAACGCATCGCCAATGGTGATCGCTCCCCCATATTGTCAGCTCATCCCATCTCAGAATTCCTCACCAGTTCTGGGACTTCAGCTGGAGAGAGGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GH3

Protein Analysis

117

Amino Acids

12.65

Weight (kDa)

8.62

Isoelectric Point (pI)

73.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GH3 PF03321 22 - 78 1.1e-18 GH3 family N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0029270)

Species Orthologous Gene IDs
pyrus_communis pycom05g09200
rosa_samantha Rh6BG061800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 179
AccBSI CCGCTC 1 cut(s) 112
AccI GTMKAC 1 cut(s) 9
AciI CCGC 3 cut(s) 112, 177, 190
AclWI GGATC 2 cut(s) 231, 240
AcsI RAATTY 1 cut(s) 307
AcuI CTGAAG 1 cut(s) 314
AcyI GRCGYC 3 cut(s) 54, 99, 180
AfaI GTAC 1 cut(s) 155
AfiI CCNNNNNNNGG 1 cut(s) 323
AgsI TTSAA 3 cut(s) 77, 169, 202
AluBI AGCT 4 cut(s) 134, 292, 334, 348
AluI AGCT 4 cut(s) 134, 292, 334, 348
AlwI GGATC 2 cut(s) 231, 240
AoxI GGCC 2 cut(s) 31, 39
ApoI RAATTY 1 cut(s) 307
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 1 cut(s) 32
AsuC2I CCSGG 1 cut(s) 88
AsuHPI GGTGA 2 cut(s) 280, 306
BanI GGYRCC 1 cut(s) 179
BccI CCATC 1 cut(s) 307
BceAI ACGGC 1 cut(s) 86
BcnI CCSGG 1 cut(s) 88
BfaI CTAG 1 cut(s) 131
BfoI RGCGCY 1 cut(s) 183
BisI GCNGC 1 cut(s) 178
BlsI GCNGC 1 cut(s) 179
Bme1390I CCNGG 1 cut(s) 88
BmgT120I GGNCC 1 cut(s) 32
BmiI GGNNCC 1 cut(s) 181
BmrFI CCNGG 1 cut(s) 88
BmsI GCATC 1 cut(s) 266
BpuMI CCSGG 1 cut(s) 88
Bsa29I ATCGAT 1 cut(s) 235
BsaHI GRCGYC 3 cut(s) 54, 99, 180
BsaJI CCNNGG 1 cut(s) 28
Bsc4I CCNNNNNNNGG 1 cut(s) 323
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 1 cut(s) 317
BseCI ATCGAT 1 cut(s) 235
BseDI CCNNGG 1 cut(s) 28
BseGI GGATG 1 cut(s) 294
BseLI CCNNNNNNNGG 1 cut(s) 323
BseMII CTCAG 1 cut(s) 317
BseNI ACTGG 1 cut(s) 317
BseRI GAGGAG 1 cut(s) 4
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 2 cut(s) 33, 41
BshNI GGYRCC 1 cut(s) 179
BshVI ATCGAT 1 cut(s) 235
BsiEI CGRYCG 1 cut(s) 190
BsiSI CCGG 2 cut(s) 38, 88
BslFI GGGAC 1 cut(s) 339
BslI CCNNNNNNNGG 1 cut(s) 323
BsmFI GGGAC 1 cut(s) 339
BsnI GGCC 2 cut(s) 33, 41
Bsp143I GATC 3 cut(s) 232, 236, 270
BspACI CCGC 3 cut(s) 112, 177, 190
BspANI GGCC 2 cut(s) 33, 41
BspCNI CTCAG 1 cut(s) 316
BspDI ATCGAT 1 cut(s) 235
BspLI GGNNCC 1 cut(s) 181
BspPI GGATC 2 cut(s) 231, 240
BspT107I GGYRCC 1 cut(s) 179
BsrBI CCGCTC 1 cut(s) 112
BsrFI RCCGGY 1 cut(s) 37
BsrI ACTGG 1 cut(s) 317
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 1 cut(s) 28
BssMI GATC 3 cut(s) 232, 236, 270
BssNI GRCGYC 3 cut(s) 54, 99, 180
Bst4CI ACNGT 1 cut(s) 69
BstACI GRCGYC 3 cut(s) 54, 99, 180
BstC8I GCNNGC 1 cut(s) 43
BstDEI CTNAG 1 cut(s) 303
BstF5I GGATG 1 cut(s) 294
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 3 cut(s) 235, 239, 273
BstMBI GATC 3 cut(s) 232, 236, 270
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 1 cut(s) 140
BstSCI CCNGG 1 cut(s) 86
Bsu15I ATCGAT 1 cut(s) 235
BsuRI GGCC 2 cut(s) 33, 41
BsuTUI ATCGAT 1 cut(s) 235
BtgZI GCGATG 1 cut(s) 243
BtsCI GGATG 1 cut(s) 294
Cac8I GCNNGC 1 cut(s) 43
CfoI GCGC 1 cut(s) 182
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 1 cut(s) 32
ClaI ATCGAT 1 cut(s) 235
CseI GACGC 2 cut(s) 62, 107
Csp6I GTAC 1 cut(s) 154
CviJI RGCY 8 cut(s) 5, 33, 41, 134, 243, 292, 334, 348
CviKI_1 RGCY 8 cut(s) 5, 33, 41, 134, 243, 292, 334, 348
CviQI GTAC 1 cut(s) 154
DdeI CTNAG 1 cut(s) 303
DinI GGCGCC 1 cut(s) 181
DpnI GATC 3 cut(s) 234, 238, 272
DpnII GATC 3 cut(s) 232, 236, 270
Eco57I CTGAAG 1 cut(s) 314
EcoRI GAATTC 1 cut(s) 307
EgeI GGCGCC 1 cut(s) 181
EheI GGCGCC 1 cut(s) 181
FaiI YATR 1 cut(s) 283
FaqI GGGAC 1 cut(s) 339
FauI CCCGC 1 cut(s) 105
FblI GTMKAC 1 cut(s) 9
Fnu4HI GCNGC 1 cut(s) 178
FokI GGATG 1 cut(s) 281
Fsp4HI GCNGC 1 cut(s) 178
FspBI CTAG 1 cut(s) 131
GlaI GCGC 1 cut(s) 181
GluI GCNGC 1 cut(s) 178
HaeII RGCGCY 1 cut(s) 183
HaeIII GGCC 2 cut(s) 33, 41
HapII CCGG 2 cut(s) 38, 88
HgaI GACGC 2 cut(s) 62, 107
HhaI GCGC 1 cut(s) 182
Hin1I GRCGYC 3 cut(s) 54, 99, 180
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HincII GTYRAC 1 cut(s) 10
HindII GTYRAC 1 cut(s) 10
HinfI GANTC 1 cut(s) 11
HpaII CCGG 2 cut(s) 38, 88
HphI GGTGA 2 cut(s) 280, 306
Hpy166II GTNNAC 1 cut(s) 10
Hpy188I TCNGA 2 cut(s) 124, 306
Hpy8I GTNNAC 1 cut(s) 10
Hpy99I CGWCG 1 cut(s) 101
HpyCH4III ACNGT 1 cut(s) 69
HpyCH4V TGCA 1 cut(s) 106
HpyF10VI GCNNNNNNNGC 1 cut(s) 140
HpyF3I CTNAG 1 cut(s) 303
Hsp92I GRCGYC 3 cut(s) 54, 99, 180
HspAI GCGC 1 cut(s) 180
KasI GGCGCC 1 cut(s) 179
Kzo9I GATC 3 cut(s) 232, 236, 270
LmnI GCTCC 1 cut(s) 280
LpnPI CCDG 8 cut(s) 51, 55, 101, 158, 253, 308, 320, 330
LweI GCATC 1 cut(s) 266
MaeI CTAG 1 cut(s) 131
MaeIII GTNAC 1 cut(s) 63
MalI GATC 3 cut(s) 234, 238, 272
MbiI CCGCTC 1 cut(s) 112
MboI GATC 3 cut(s) 232, 236, 270
MboII GAAGA 1 cut(s) 89
MluCI AATT 1 cut(s) 307
Mly113I GGCGCC 1 cut(s) 180
MlyI GAGTC 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 276
MnlI CCTC 5 cut(s) 25, 38, 223, 322, 335
MseI TTAA 1 cut(s) 159
MspA1I CMGCKG 1 cut(s) 334
MspI CCGG 2 cut(s) 38, 88
MspR9I CCNGG 1 cut(s) 88
MwoI GCNNNNNNNGC 1 cut(s) 140
NarI GGCGCC 1 cut(s) 180
NciI CCSGG 1 cut(s) 88
NdeII GATC 3 cut(s) 232, 236, 270
NlaIV GGNNCC 1 cut(s) 181
NmeAIII GCCGAG 2 cut(s) 9, 153
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PkrI GCNGC 1 cut(s) 179
PleI GAGTC 1 cut(s) 5
PluTI GGCGCC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 5
PspN4I GGNNCC 1 cut(s) 181
PspPI GGNCC 1 cut(s) 32
PvuII CAGCTG 1 cut(s) 334
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
SalI GTCGAC 1 cut(s) 8
SaqAI TTAA 1 cut(s) 159
SatI GCNGC 1 cut(s) 178
Sau3AI GATC 3 cut(s) 232, 236, 270
Sau96I GGNCC 1 cut(s) 32
SchI GAGTC 1 cut(s) 5
ScrFI CCNGG 1 cut(s) 88
SetI ASST 6 cut(s) 136, 159, 227, 294, 336, 350
SfaNI GCATC 1 cut(s) 266
SfoI GGCGCC 1 cut(s) 181
Sse9I AATT 1 cut(s) 307
SsiI CCGC 3 cut(s) 112, 177, 190
SspDI GGCGCC 1 cut(s) 179
SspMI CTAG 1 cut(s) 131
StyD4I CCNGG 1 cut(s) 86
TaaI ACNGT 1 cut(s) 69
TaqI TCGA 3 cut(s) 9, 136, 235
TasI AATT 1 cut(s) 307
TauI GCSGC 1 cut(s) 180
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TspDTI ATGAA 1 cut(s) 61
XapI RAATTY 1 cut(s) 307
XmiI GTMKAC 1 cut(s) 9
XspI CTAG 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.