Rh6BG061800

Indole-3-acetic acid-amido synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0029270
Physical Location & Seq
Reverse (-)
9759631 .. 9760260
630 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG061800.1

Sequence Viewer

Length: 426 bp
ATGGCAGTTGACTCGTCCCTTCCTTCTCCCCTCGGGCCACCGGCCTGTGAGAAAGACGCCAAGGCCTTGCAATACATCGAACAAACCACCCGAAACGCCGACGCCGTCCAGGAGACGGTGCTCGCCGAAATTCTCAGCCGAAACGCCGAGACCGAATACCTCCGACGCTACAAGCTCGGCGGCGCCACTGACCGTAAATCCTTTAAATCCAAGCTTCCAGTCATCACTTACGAGGATGTCCAGCCGGAAATCCAAAGGATCGCCAATGGAGATCGCTCCCCTATCCTCTCGGCTCATCCCATCTCTGAGTTTCTCACCAGCTCTGGTACTTCAGCGGGTGAGAGGAAGCTGATGCCAACGATTCAGGAGGAGTTGGATCGTCGTCAATCACTCTACAACCTCCTCATGCCAGTCATGAACCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GH3

Protein Analysis

141

Amino Acids

15.69

Weight (kDa)

5.49

Isoelectric Point (pI)

73.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GH3 PF03321 23 - 140 5e-46 GH3 family N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029270)

Species Orthologous Gene IDs
pyrus_communis pycom05g09200
rosa_samantha Rh6BG061800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 182
AciI CCGC 2 cut(s) 180, 335
AclWI GGATC 2 cut(s) 266, 384
AcsI RAATTY 1 cut(s) 129
AcuI CTGAAG 1 cut(s) 315
AcyI GRCGYC 3 cut(s) 57, 102, 183
AfaI GTAC 1 cut(s) 328
AfiI CCNNNNNNNGG 1 cut(s) 115
AjnI CCWGG 1 cut(s) 108
AluBI AGCT 4 cut(s) 175, 214, 321, 349
AluI AGCT 4 cut(s) 175, 214, 321, 349
Alw21I GWGCWC 1 cut(s) 123
Alw26I GTCTC 2 cut(s) 107, 143
AlwI GGATC 2 cut(s) 266, 384
Ama87I CYCGRG 1 cut(s) 32
AoxI GGCC 3 cut(s) 35, 42, 63
ApoI RAATTY 1 cut(s) 129
AspLEI GCGC 1 cut(s) 185
AspS9I GGNCC 1 cut(s) 35
AsuHPI GGTGA 2 cut(s) 307, 350
AvaI CYCGRG 1 cut(s) 32
BanI GGYRCC 1 cut(s) 182
Bbv12I GWGCWC 1 cut(s) 123
BccI CCATC 1 cut(s) 308
BceAI ACGGC 1 cut(s) 89
BcgI CGANNNNNNTGC 1 cut(s) 28
BciT130I CCWGG 1 cut(s) 110
BcoDI GTCTC 2 cut(s) 107, 143
BfoI RGCGCY 1 cut(s) 186
BisI GCNGC 1 cut(s) 181
BlsI GCNGC 1 cut(s) 182
Bme1390I CCNGG 1 cut(s) 110
BmeT110I CYCGRG 1 cut(s) 32
BmgT120I GGNCC 1 cut(s) 35
BmiI GGNNCC 1 cut(s) 184
BmrFI CCNGG 1 cut(s) 110
BmsI GCATC 1 cut(s) 342
BsaHI GRCGYC 3 cut(s) 57, 102, 183
BsaI GGTCTC 1 cut(s) 143
BsaJI CCNNGG 2 cut(s) 31, 60
Bsc4I CCNNNNNNNGG 1 cut(s) 115
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 2 cut(s) 218, 410
BseBI CCWGG 1 cut(s) 110
BseDI CCNNGG 2 cut(s) 31, 60
BseGI GGATG 2 cut(s) 241, 295
BseLI CCNNNNNNNGG 1 cut(s) 115
BseMII CTCAG 2 cut(s) 148, 297
BseNI ACTGG 2 cut(s) 218, 410
BseRI GAGGAG 2 cut(s) 383, 392
BshFI GGCC 3 cut(s) 37, 44, 65
BshNI GGYRCC 1 cut(s) 182
BsiHKAI GWGCWC 1 cut(s) 123
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 2 cut(s) 41, 245
BslI CCNNNNNNNGG 1 cut(s) 115
BsmAI GTCTC 2 cut(s) 107, 143
BsmBI CGTCTC 1 cut(s) 107
BsnI GGCC 3 cut(s) 37, 44, 65
Bso31I GGTCTC 1 cut(s) 143
BsoBI CYCGRG 1 cut(s) 32
Bsp1286I GDGCHC 1 cut(s) 123
Bsp143I GATC 3 cut(s) 258, 271, 376
BspACI CCGC 2 cut(s) 180, 335
BspANI GGCC 3 cut(s) 37, 44, 65
BspCNI CTCAG 2 cut(s) 147, 298
BspHI TCATGA 1 cut(s) 414
BspLI GGNNCC 1 cut(s) 184
BspPI GGATC 2 cut(s) 266, 384
BspT107I GGYRCC 1 cut(s) 182
BspTNI GGTCTC 1 cut(s) 143
BsrFI RCCGGY 1 cut(s) 40
BsrI ACTGG 2 cut(s) 218, 410
BssAI RCCGGY 1 cut(s) 40
BssECI CCNNGG 2 cut(s) 31, 60
BssMI GATC 3 cut(s) 258, 271, 376
BssNI GRCGYC 3 cut(s) 57, 102, 183
BssT1I CCWWGG 1 cut(s) 60
Bst2UI CCWGG 1 cut(s) 110
Bst4CI ACNGT 2 cut(s) 118, 194
BstACI GRCGYC 3 cut(s) 57, 102, 183
BstC8I GCNNGC 1 cut(s) 123
BstDEI CTNAG 2 cut(s) 134, 306
BstF5I GGATG 2 cut(s) 241, 295
BstH2I RGCGCY 1 cut(s) 186
BstHHI GCGC 1 cut(s) 185
BstKTI GATC 3 cut(s) 261, 274, 379
BstMAI GTCTC 2 cut(s) 107, 143
BstMBI GATC 3 cut(s) 258, 271, 376
BstNI CCWGG 1 cut(s) 110
BstSCI CCNGG 1 cut(s) 108
BsuRI GGCC 3 cut(s) 37, 44, 65
BtsCI GGATG 2 cut(s) 241, 295
BtsIMutI CAGTG 1 cut(s) 186
Cac8I GCNNGC 1 cut(s) 123
CciI TCATGA 1 cut(s) 414
CfoI GCGC 1 cut(s) 185
Cfr10I RCCGGY 1 cut(s) 40
Cfr13I GGNCC 1 cut(s) 35
CseI GACGC 3 cut(s) 65, 110, 174
Csp6I GTAC 1 cut(s) 327
CviAII CATG 2 cut(s) 406, 415
CviQI GTAC 1 cut(s) 327
DdeI CTNAG 2 cut(s) 134, 306
DinI GGCGCC 1 cut(s) 184
DpnI GATC 3 cut(s) 260, 273, 378
DpnII GATC 3 cut(s) 258, 271, 376
DraI TTTAAA 1 cut(s) 205
Eco130I CCWWGG 1 cut(s) 60
Eco147I AGGCCT 1 cut(s) 65
Eco31I GGTCTC 1 cut(s) 143
Eco57I CTGAAG 1 cut(s) 315
Eco88I CYCGRG 1 cut(s) 32
EcoRII CCWGG 1 cut(s) 108
EcoT14I CCWWGG 1 cut(s) 60
EgeI GGCGCC 1 cut(s) 184
EheI GGCGCC 1 cut(s) 184
ErhI CCWWGG 1 cut(s) 60
Esp3I CGTCTC 1 cut(s) 107
FaeI CATG 2 cut(s) 409, 418
FaiI YATR 2 cut(s) 407, 416
FatI CATG 2 cut(s) 405, 414
FauI CCCGC 1 cut(s) 328
Fnu4HI GCNGC 1 cut(s) 181
FokI GGATG 2 cut(s) 248, 282
Fsp4HI GCNGC 1 cut(s) 181
GlaI GCGC 1 cut(s) 184
GluI GCNGC 1 cut(s) 181
HaeII RGCGCY 1 cut(s) 186
HaeIII GGCC 3 cut(s) 37, 44, 65
HapII CCGG 2 cut(s) 41, 245
HgaI GACGC 3 cut(s) 65, 110, 174
HhaI GCGC 1 cut(s) 185
Hin1I GRCGYC 3 cut(s) 57, 102, 183
Hin1II CATG 2 cut(s) 409, 418
Hin6I GCGC 1 cut(s) 183
HinP1I GCGC 1 cut(s) 183
HincII GTYRAC 1 cut(s) 10
HindII GTYRAC 1 cut(s) 10
HindIII AAGCTT 1 cut(s) 212
HinfI GANTC 2 cut(s) 11, 361
HpaII CCGG 2 cut(s) 41, 245
HphI GGTGA 2 cut(s) 307, 350
Hpy166II GTNNAC 1 cut(s) 10
Hpy188I TCNGA 2 cut(s) 164, 307
Hpy188III TCNNGA 2 cut(s) 365, 415
Hpy8I GTNNAC 1 cut(s) 10
Hpy99I CGWCG 3 cut(s) 104, 168, 384
HpyAV CCTTC 2 cut(s) 29, 33
HpyCH4III ACNGT 2 cut(s) 118, 194
HpyCH4V TGCA 1 cut(s) 70
HpyF3I CTNAG 2 cut(s) 134, 306
Hsp92I GRCGYC 3 cut(s) 57, 102, 183
Hsp92II CATG 2 cut(s) 409, 418
HspAI GCGC 1 cut(s) 183
KasI GGCGCC 1 cut(s) 182
Kzo9I GATC 3 cut(s) 258, 271, 376
LmnI GCTCC 1 cut(s) 281
LweI GCATC 1 cut(s) 342
MalI GATC 3 cut(s) 260, 273, 378
MboI GATC 3 cut(s) 258, 271, 376
MhlI GDGCHC 1 cut(s) 123
MluCI AATT 1 cut(s) 129
Mly113I GGCGCC 1 cut(s) 183
MlyI GAGTC 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 187, 354
MnlI CCTC 8 cut(s) 41, 170, 226, 296, 336, 361, 410, 413
MseI TTAA 1 cut(s) 204
MspA1I CMGCKG 1 cut(s) 335
MspI CCGG 2 cut(s) 41, 245
MspR9I CCNGG 1 cut(s) 110
MvaI CCWGG 1 cut(s) 110
NarI GGCGCC 1 cut(s) 183
NdeII GATC 3 cut(s) 258, 271, 376
NlaIII CATG 2 cut(s) 409, 418
NlaIV GGNNCC 1 cut(s) 184
NmeAIII GCCGAG 3 cut(s) 156, 172, 269
PagI TCATGA 1 cut(s) 414
PceI AGGCCT 1 cut(s) 65
PcsI WCGNNNNNNNCGW 2 cut(s) 102, 150
PfeI GAWTC 1 cut(s) 361
PflFI GACNNNGTC 1 cut(s) 104
PfoI TCCNGGA 1 cut(s) 108
PkrI GCNGC 1 cut(s) 182
PleI GAGTC 1 cut(s) 5
PluTI GGCGCC 1 cut(s) 186
PpsI GAGTC 1 cut(s) 5
Psp6I CCWGG 1 cut(s) 108
PspGI CCWGG 1 cut(s) 108
PspN4I GGNNCC 1 cut(s) 184
PspPI GGNCC 1 cut(s) 35
PsyI GACNNNGTC 1 cut(s) 104
RsaI GTAC 1 cut(s) 328
RsaNI GTAC 1 cut(s) 327
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 1 cut(s) 181
Sau3AI GATC 3 cut(s) 258, 271, 376
Sau96I GGNCC 1 cut(s) 35
SchI GAGTC 1 cut(s) 5
ScrFI CCNGG 1 cut(s) 110
SduI GDGCHC 1 cut(s) 123
SetI ASST 7 cut(s) 162, 177, 216, 323, 351, 402, 423
SfaNI GCATC 1 cut(s) 342
SfoI GGCGCC 1 cut(s) 184
Sse9I AATT 1 cut(s) 129
SseBI AGGCCT 1 cut(s) 65
SsiI CCGC 2 cut(s) 180, 335
SspDI GGCGCC 1 cut(s) 182
StuI AGGCCT 1 cut(s) 65
StyD4I CCNGG 1 cut(s) 108
StyI CCWWGG 1 cut(s) 60
TaaI ACNGT 2 cut(s) 118, 194
TaqI TCGA 1 cut(s) 78
TaqII GACCGA 1 cut(s) 167
TasI AATT 1 cut(s) 129
TauI GCSGC 1 cut(s) 183
TfiI GAWTC 1 cut(s) 361
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TscAI CASTG 1 cut(s) 193
TspRI CASTG 1 cut(s) 193
Tth111I GACNNNGTC 1 cut(s) 104
XapI RAATTY 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.