pycom05g13460

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0028813
Physical Location & Seq
Reverse (-)
17011443 .. 17012584
1142 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 645 bp
ATGAAAGGGTCTCAAAACTATAATGCTGCTAACGATCCTGATCTCTTATCTGCTTTTTGGATTTTTAATGGCCCTGGTGGTAGATATGCAGCTTTTGTTATAAGGGAGCACATTTTTAACTTCATAGTTGAGGATCCATATTTCACCTTTACCATTAAGAAGGCAAGTAGGGAAGCTTTCAAAAAAGCTGGCCATGCTTTTGCTGATATTGGTACTCCTAATAGTTCCTCCATCACCAGTGCACTTTCTGTCCTCGTCTTGGGAAGGAAGATGCTAATTGCGAATGTTGGGGATTGTCTAGTTGTGATGGGAAGAAGAGATAAGGAAGTTGTGCTGATTTCCGAAGACCTAGAAAGCTCCAAAGGTTCCTCATGTCCTTTGCCTGCACCGCCAAAAGTTCAGGAGATATTTCTGAGCAAGGAAGATGAGTTCTTGATCCTAACCTGGAATGACTATAATATTTGCCGCCCGCATTTATTTACACATGCAAGGAAAGAATTAATGGATAACAATGATCCAGGGAGATGCGCAAGAGAGATTGTTAGGAAGGCACTCTATTGGGAGTGGGGTACCAACATGAAGGATGGGGTGGTATGCTTCTCGCCCAACCCACCGCCACCTTTGTCACCTTTGTTTAGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

215

Amino Acids

24.05

Weight (kDa)

7.64

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 5 - 116 1.5e-08 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028813)

Species Orthologous Gene IDs
malus_domestica MD05G1139500.v1.1
pyrus_communis pycom05g13460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 101
Acc16I TGCGCA 1 cut(s) 529
Acc65I GGTACC 1 cut(s) 569
AccB1I GGYRCC 1 cut(s) 569
AciI CCGC 4 cut(s) 389, 466, 470, 614
AclWI GGATC 5 cut(s) 29, 128, 141, 430, 509
AcoI YGGCCR 1 cut(s) 190
AfaI GTAC 2 cut(s) 214, 571
AfiI CCNNNNNNNGG 1 cut(s) 259
AgsI TTSAA 1 cut(s) 181
AjnI CCWGG 3 cut(s) 73, 443, 517
AloI GAACNNNNNNTCC 2 cut(s) 413, 445
AluBI AGCT 4 cut(s) 92, 176, 188, 357
AluI AGCT 4 cut(s) 92, 176, 188, 357
Alw21I GWGCWC 2 cut(s) 111, 244
Alw26I GTCTC 1 cut(s) 15
Alw44I GTGCAC 1 cut(s) 240
AlwI GGATC 5 cut(s) 29, 128, 141, 430, 509
AoxI GGCC 2 cut(s) 70, 190
ApaLI GTGCAC 1 cut(s) 240
ApeKI GCWGC 2 cut(s) 26, 89
AseI ATTAAT 1 cut(s) 500
Asp718I GGTACC 1 cut(s) 569
AspLEI GCGC 1 cut(s) 530
AspS9I GGNCC 1 cut(s) 71
AsuHPI GGTGA 3 cut(s) 136, 226, 618
BaeGI GKGCMC 1 cut(s) 244
BalI TGGCCA 1 cut(s) 192
BamHI GGATCC 1 cut(s) 133
BanI GGYRCC 1 cut(s) 569
BbsI GAAGAC 1 cut(s) 351
Bbv12I GWGCWC 2 cut(s) 111, 244
BbvI GCAGC 2 cut(s) 13, 101
BccI CCATC 3 cut(s) 239, 301, 578
BciT130I CCWGG 3 cut(s) 75, 445, 519
BcoDI GTCTC 1 cut(s) 15
BfaI CTAG 2 cut(s) 299, 350
BisI GCNGC 3 cut(s) 27, 90, 466
BlsI GCNGC 3 cut(s) 28, 91, 467
Bme1390I CCNGG 3 cut(s) 75, 445, 519
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 3 cut(s) 135, 367, 571
BmrFI CCNGG 3 cut(s) 75, 445, 519
BmsI GCATC 2 cut(s) 261, 515
BpiI GAAGAC 1 cut(s) 351
BsaBI GATNNNNATC 1 cut(s) 39
BsaI GGTCTC 1 cut(s) 15
BsaJI CCNNGG 2 cut(s) 73, 518
Bsc4I CCNNNNNNNGG 1 cut(s) 259
Bse1I ACTGG 1 cut(s) 237
Bse8I GATNNNNATC 1 cut(s) 39
BseBI CCWGG 3 cut(s) 75, 445, 519
BseDI CCNNGG 2 cut(s) 73, 518
BseGI GGATG 1 cut(s) 589
BseJI GATNNNNATC 1 cut(s) 39
BseLI CCNNNNNNNGG 1 cut(s) 259
BseMII CTCAG 1 cut(s) 404
BseNI ACTGG 1 cut(s) 237
BseSI GKGCMC 1 cut(s) 244
BseXI GCAGC 2 cut(s) 13, 101
BsgI GTGCAG 1 cut(s) 369
BshFI GGCC 2 cut(s) 72, 192
BshNI GGYRCC 1 cut(s) 569
BsiHKAI GWGCWC 2 cut(s) 111, 244
BslI CCNNNNNNNGG 1 cut(s) 259
BsmAI GTCTC 1 cut(s) 15
BsnI GGCC 2 cut(s) 72, 192
Bso31I GGTCTC 1 cut(s) 15
Bsp1286I GDGCHC 2 cut(s) 111, 244
Bsp143I GATC 5 cut(s) 34, 40, 133, 435, 514
BspACI CCGC 4 cut(s) 389, 466, 470, 614
BspANI GGCC 2 cut(s) 72, 192
BspCNI CTCAG 1 cut(s) 405
BspLI GGNNCC 3 cut(s) 135, 367, 571
BspPI GGATC 5 cut(s) 29, 128, 141, 430, 509
BspT107I GGYRCC 1 cut(s) 569
BspTNI GGTCTC 1 cut(s) 15
BsrI ACTGG 1 cut(s) 237
BssECI CCNNGG 2 cut(s) 73, 518
BssMI GATC 5 cut(s) 34, 40, 133, 435, 514
Bst2UI CCWGG 3 cut(s) 75, 445, 519
Bst6I CTCTTC 1 cut(s) 310
BstC8I GCNNGC 3 cut(s) 190, 384, 470
BstDEI CTNAG 1 cut(s) 413
BstF5I GGATG 1 cut(s) 589
BstHHI GCGC 1 cut(s) 530
BstKTI GATC 5 cut(s) 37, 43, 136, 438, 517
BstMAI GTCTC 1 cut(s) 15
BstMBI GATC 5 cut(s) 34, 40, 133, 435, 514
BstMWI GCNNNNNNNGC 2 cut(s) 194, 388
BstNI CCWGG 3 cut(s) 75, 445, 519
BstNSI RCATGY 1 cut(s) 488
BstSCI CCNGG 3 cut(s) 73, 443, 517
BstSLI GKGCMC 1 cut(s) 244
BstV1I GCAGC 2 cut(s) 13, 101
BstV2I GAAGAC 1 cut(s) 351
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
BsuRI GGCC 2 cut(s) 72, 192
BtsCI GGATG 1 cut(s) 589
BtsIMutI CAGTG 1 cut(s) 244
Cac8I GCNNGC 3 cut(s) 190, 384, 470
CfoI GCGC 1 cut(s) 530
Cfr13I GGNCC 1 cut(s) 71
Csp6I GTAC 2 cut(s) 213, 570
CviAII CATG 4 cut(s) 194, 372, 485, 577
CviJI RGCY 6 cut(s) 72, 92, 176, 188, 192, 357
CviKI_1 RGCY 6 cut(s) 72, 92, 176, 188, 192, 357
CviQI GTAC 2 cut(s) 213, 570
DdeI CTNAG 1 cut(s) 413
DpnI GATC 5 cut(s) 36, 42, 135, 437, 516
DpnII GATC 5 cut(s) 34, 40, 133, 435, 514
EaeI YGGCCR 1 cut(s) 190
Eam1104I CTCTTC 1 cut(s) 310
EarI CTCTTC 1 cut(s) 310
Eco31I GGTCTC 1 cut(s) 15
EcoRII CCWGG 3 cut(s) 73, 443, 517
FaeI CATG 4 cut(s) 197, 375, 488, 580
FatI CATG 4 cut(s) 193, 371, 484, 576
FauI CCCGC 1 cut(s) 477
Fnu4HI GCNGC 3 cut(s) 27, 90, 466
FokI GGATG 1 cut(s) 596
Fsp4HI GCNGC 3 cut(s) 27, 90, 466
FspBI CTAG 2 cut(s) 299, 350
FspI TGCGCA 1 cut(s) 529
GlaI GCGC 1 cut(s) 529
GluI GCNGC 3 cut(s) 27, 90, 466
HaeIII GGCC 2 cut(s) 72, 192
HhaI GCGC 1 cut(s) 530
Hin1II CATG 4 cut(s) 197, 375, 488, 580
Hin6I GCGC 1 cut(s) 528
HinP1I GCGC 1 cut(s) 528
HindIII AAGCTT 1 cut(s) 174
HphI GGTGA 3 cut(s) 136, 226, 618
Hpy166II GTNNAC 1 cut(s) 242
Hpy188I TCNGA 2 cut(s) 343, 414
Hpy188III TCNNGA 3 cut(s) 38, 401, 433
Hpy8I GTNNAC 1 cut(s) 242
HpyAV CCTTC 4 cut(s) 154, 258, 541, 574
HpyCH4V TGCA 4 cut(s) 89, 242, 386, 488
HpyF10VI GCNNNNNNNGC 2 cut(s) 194, 388
HpyF3I CTNAG 1 cut(s) 413
Hsp92II CATG 4 cut(s) 197, 375, 488, 580
HspAI GCGC 1 cut(s) 528
KpnI GGTACC 1 cut(s) 573
Kzo9I GATC 5 cut(s) 34, 40, 133, 435, 514
LmnI GCTCC 2 cut(s) 106, 362
Lsp1109I GCAGC 2 cut(s) 13, 101
LweI GCATC 2 cut(s) 261, 515
MaeI CTAG 2 cut(s) 299, 350
MaeIII GTNAC 1 cut(s) 624
MalI GATC 5 cut(s) 36, 42, 135, 437, 516
MboI GATC 5 cut(s) 34, 40, 133, 435, 514
MboII GAAGA 5 cut(s) 280, 324, 327, 356, 434
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 2 cut(s) 111, 244
MlsI TGGCCA 1 cut(s) 192
MluCI AATT 2 cut(s) 276, 497
MluNI TGGCCA 1 cut(s) 192
MnlI CCTC 4 cut(s) 124, 238, 263, 379
Mox20I TGGCCA 1 cut(s) 192
MscI TGGCCA 1 cut(s) 192
MseI TTAA 4 cut(s) 66, 117, 156, 500
Msp20I TGGCCA 1 cut(s) 192
MspR9I CCNGG 3 cut(s) 75, 445, 519
MvaI CCWGG 3 cut(s) 75, 445, 519
MwoI GCNNNNNNNGC 2 cut(s) 194, 388
NdeII GATC 5 cut(s) 34, 40, 133, 435, 514
NlaIII CATG 4 cut(s) 197, 375, 488, 580
NlaIV GGNNCC 3 cut(s) 135, 367, 571
NmuCI GTSAC 1 cut(s) 624
NsbI TGCGCA 1 cut(s) 529
NspI RCATGY 1 cut(s) 488
PkrI GCNGC 3 cut(s) 28, 91, 467
PshBI ATTAAT 1 cut(s) 500
PsiI TTATAA 1 cut(s) 101
Psp6I CCWGG 3 cut(s) 73, 443, 517
PspGI CCWGG 3 cut(s) 73, 443, 517
PspN4I GGNNCC 3 cut(s) 135, 367, 571
PspPI GGNCC 1 cut(s) 71
PsuI RGATCY 1 cut(s) 133
RsaI GTAC 2 cut(s) 214, 571
RsaNI GTAC 2 cut(s) 213, 570
SaqAI TTAA 4 cut(s) 66, 117, 156, 500
SatI GCNGC 3 cut(s) 27, 90, 466
Sau3AI GATC 5 cut(s) 34, 40, 133, 435, 514
Sau96I GGNCC 1 cut(s) 71
ScrFI CCNGG 3 cut(s) 75, 445, 519
SduI GDGCHC 2 cut(s) 111, 244
SfaNI GCATC 2 cut(s) 261, 515
Sse9I AATT 2 cut(s) 276, 497
SsiI CCGC 4 cut(s) 389, 466, 470, 614
SspI AATATT 1 cut(s) 460
SspMI CTAG 2 cut(s) 299, 350
StyD4I CCNGG 3 cut(s) 73, 443, 517
TasI AATT 2 cut(s) 276, 497
TauI GCSGC 1 cut(s) 468
Tru1I TTAA 4 cut(s) 66, 117, 156, 500
Tru9I TTAA 4 cut(s) 66, 117, 156, 500
TscAI CASTG 1 cut(s) 244
TseFI GTSAC 1 cut(s) 624
TseI GCWGC 2 cut(s) 26, 89
Tsp45I GTSAC 1 cut(s) 624
TspDTI ATGAA 3 cut(s) 17, 112, 593
TspRI CASTG 1 cut(s) 244
VneI GTGCAC 1 cut(s) 240
VspI ATTAAT 1 cut(s) 500
XceI RCATGY 1 cut(s) 488
XspI CTAG 2 cut(s) 299, 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.