pycom05g18490

Sugar (and other) transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0015788
Physical Location & Seq
Reverse (-)
21395552 .. 21400591
5040 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g18490.1

Sequence Viewer

Length: 3105 bp
ATGCATATCGATCTCTTCTGCATATCTTTGGTGACCAAGTTGCTCGGCCGCATATACTACCACGTTGACAACGCAGAGAAGCCCGGGACATTGCCTCCGAATGTGTCAGCTGCCGTAAATGGTGTGGCGTTTTGTGGAACCTTAGCAGGCCAGCTCTTCTTTGGCTGGCTTGGTGACAAGATGGGAAGGAAGAGAGTTTATGGAATGACTCTTATGCTTATGGTCATATGTTCTATTGCTTCAGGTCTCTCATTTGGGCATACCCCAAAAACCGTTATGTCAACGCTTTGTTTCTTCCGGTTCTGGCTAGGGTTTGGTATTGGCGGTGACTACCCTCTTTCTGCCACAATCATGTCCGAGTATGCTAACAAGAAGACTCGGGGCGCTTTCATTGCTGCGGTCTTTGCCATGCAGGGGTTTGGAATTTTAGCAGGTGGGATATTTGCTATTATCTGTTCGGCCGCGTTTAATGCCAATTTTGATGCTCCAACATATGAGGTCAATGCACTTGCCTCAACCGTTCCACAAGCGGACTACCTGTGGAGGATTATTGTGATGGTAGGAGCAATCCCAGCTGCAATGACTTACTACTGGCGCATGAAGATGCCTGAAACTGCCCGTTACACCGCCCTTGTTGCAAAGAATGCGAAACAGGCTGCATCCGACATGTCAAGGGTTCTGCAGGTAGAAATTCAAGCAGAACCCCAGAAGGCTGAGCCGACTGCTAATGCATTTGCTTTGTTCTCCAAGGAGTTTATGCACCGTCATGGACTTCACTTGCTTGGAACAACAAGCACTTGGTTCTTGCTTGACATTGCATTCTACAGCCAAAACCTGTTCCAAAAGGATATTTTCAGTGCGATCGGATGGATTCCTCCTGCAAAGACGATGAATGCTATTGAAGAAGTTTACAGAATCGCAAGGGCGCAAACTCTTATTGCATTGTGCAGTACTGTCCCCGGCTACTGGTTTACGGTAGCTTTCATTGACAGGATTGGAAGATTTGCAATTCAGTTGATGGGATTCTTCTTCATGACAGTGTTCATGTTTGCACTGGCTATTCCCTACGATCACTGGACTCACAAGGACAACCGAATTGGGTTTGTAGTGATCTACTCATTGACCTTCTTTTTTGCAAACTTCGGTCCTAATGCAACCACATTTGTTGTGCCGGCTGAGATTTTCCCAGCTAGGTTCCGGTCTACGTGTCATGGAATCTCAGCCGCGTCCGGGAAGCTTGGCGCCATAGTTGGTGCATTCGGGTTCTTGTACTTGGCTCAGAACAAAGATAAGAGCAAGACAGATGCAGGGTACCCTCCAGGCATCGGGGTTAAAAACTCGCTCATTGCATTGGGTGTGGTCAACTTCTTGGGGATATTGTTCACTTTCTTGGTGCCTGAATCGAATGGGAGGTCGTTGGAGGAGATGTCGGGCGAGAACGAAGAAGAAAGCGAAACCGGGGCAGTGGAGTTGGAGCAATCAAGCTATAACAACAGGACAGTTCCAGTTGTAGTTTGCCAGAGAGAGATCAAACCAAGCAAAGCTAAATCCATGGCCAAGGAACAACTGCATGTGCTCAGTGCACTTGACACTGCAAAAACACAATGGTACCATTTCACCGCAATTGTAATCGCCGGAATGGGATTCTTCACCGACGCGTACGATCTCTTCTGCATATCCCTCGTCACCAAATTGCTTGGCCGCATATATTACCACGTTGAAGGATCACCAAAACCAGGCACATTGCCTCCAAATGTGTCAGCAGCTGTCAATGGCGTGGCGCTTGTTGGCACATTGGCAGGGCAAGTGGTCTTCGGCTGGCTTGGTGACAAAATGGGGAGAAAAAAAGTGTATGGCATGACACTTATGCTCATGATCATATGCTCCATTGGTTCCGCGCTCTCCTTTGGGCACACCGCAAAGTCTGTAATGACCACACTTTGTTTCTTCCGGTTCTGGCTAGGGTTCGGCATTGGTGGTGACTACCCTCTTTCCGCCACCATAATGTCTGAATACGCAAATAAAAAGACTCGTGGTGCCTTTGTTGCCGCGGTGTTTGCCATGCAGGGGTTTGGAATTCTAGCAGGAGGGTTGTTTGCAATGCTCATCTCTGCAATTTTCGAGGCCAAATTCAAGGCTCCATCTTATGAAGTTGATCCAGTTGGTTCAACTGTCCCACAAGCAGACTACCTTTGGAGAATCATTCTAATGGTTGGAGCACTTCCGGCAGCTCTAACCTACTACTCGCGCTCAAAGATGCCAGAAACTGCTCGTTACACAGCGCTTGTTGCGAAAGATGTCAACCAGGCTAATGCTGATATGGGAAAAGTTTTGCAGGTTGACATAGAACCTGAACCCGTCAGGTTGCCTAAGGAAACTGTGAAATCTTTCGGTTTATTCTCTAAGGAGTTTATGCACCGCCATGGACTACACTTGCTTGGAACCACAAGCACATGGTTCTTGCTTGACATTGCATTTTATAGCCAAAATCTGTTTCAGAAGGACATTTTCAGCGCCATTGGTTGGATTCCTAAAGCAAAAACTTTGAATGCCGTTTCAGAGGTTTATAGAATTGCAAGGGCACAAACACTTATCGCCCTATGCAGTACTGTACCCGGATACTGGTTCACTGTAGCCTTCATTGATAAAATCGGAAGATTCACAATCCAAATGATGGGATTCTTCTTCATGACAGTGTTCATGTTTGTATTGGCATTTCTCTACGATTATTGGACACATCACTTGATAGGGTTTGTGGTATTCTACTCACTGACTTTCTTCTTTGCAAATTTCGGACCTAATGCTACTACATTTGTGGTGCCGGCTGAAATTTTCCCGGCTAGGCTGCGGTCTACTTGCCATGGCATATCTGCCGCAGCCGGAAAGTTAGGGGCAATGGTGGGTGCATTCGGATTCTTGTTCTTGGCGCAACCGCAGGACAAGGCTAAAGCAGAAGCAGGGTTTCCTGCTGGTATAGGGGTCAAGAACTCACTTATTATGTTGGGTGTGATCAACTTTTTGGGTTTATTATTTACGTTCTTTGTACCTGAATCGAAAGGAAAATCTTTGGAGGAACTGTCTGGCGAAAGCAACGAACACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0016020 GO:0016021 GO:0031224 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sugar_tr

Protein Analysis

1035

Amino Acids

113.62

Weight (kDa)

9.02

Isoelectric Point (pI)

32.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sugar_tr PF00083 9 - 477 5.2e-46 Sugar (and other) transporter
MFS_1 PF07690 38 - 419 1.9e-21 Major Facilitator Superfamily
Sugar_tr PF00083 543 - 1026 6.1e-45 Sugar (and other) transporter
MFS_1 PF07690 584 - 969 1.9e-21 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015788)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g21530
malus_domestica MD05G1198000.v1.1
prunus_persica Prupe.8G224400_v2.0.a1
pyrus_communis pycom05g18490
rosa_chinensis RchiOBHm_Chr6g0288191
rosa_laevigata RLG00000012430
rosa_multiflora Rmu_sc0002207.1_g000010
rosa_roxburghii Rroxscaffold_7G00180400
rosa_rugosa Rorug06G0194800 Rorug06G0194800
rosa_samantha Rh6AG305300 Rh6BG311200 Rh6DG304400
rosa_wichuraiana Rw0G014420 Rw6G026430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 422
Acc36I ACCTGC 3 cut(s) 422, 673, 2326
Acc65I GGTACC 2 cut(s) 1311, 1608
AccB1I GGYRCC 6 cut(s) 1241, 1311, 1393, 1608, 2036, 2818
AccB7I CCANNNNNTGG 2 cut(s) 2523, 2674
AccI GTMKAC 2 cut(s) 1202, 2852
AccII CGCG 6 cut(s) 464, 1226, 1658, 1898, 2051, 2248
AclWI GGATC 2 cut(s) 1732, 2150
AcoI YGGCCR 4 cut(s) 46, 459, 1554, 1699
AcsI RAATTY 6 cut(s) 423, 690, 2076, 2129, 2788, 2829
AcuI CTGAAG 1 cut(s) 225
AcyI GRCGYC 1 cut(s) 1242
AfaI GTAC 8 cut(s) 952, 1271, 1313, 1610, 1661, 2608, 2613, 3045
AfeI AGCGCT 1 cut(s) 2283
AfiI CCNNNNNNNGG 9 cut(s) 414, 966, 1230, 1325, 1736, 2067, 2523, 2622, 2674
AflIII ACRYGT 3 cut(s) 666, 1205, 1656
AgsI TTSAA 6 cut(s) 695, 902, 1721, 2134, 2169, 2548
AjnI CCWGG 3 cut(s) 1318, 1735, 2304
AjuI GAANNNNNNNTTGG 2 cut(s) 2478, 2510
Alw21I GWGCWC 3 cut(s) 1578, 1585, 2221
Alw26I GTCTC 1 cut(s) 251
Alw44I GTGCAC 1 cut(s) 1581
AlwI GGATC 2 cut(s) 1732, 2150
AlwNI CAGNNNCTG 2 cut(s) 1766, 2267
Ama87I CYCGRG 2 cut(s) 83, 378
Aor51HI AGCGCT 1 cut(s) 2283
AoxI GGCC 6 cut(s) 46, 148, 459, 1554, 1699, 2124
ApaLI GTGCAC 1 cut(s) 1581
ApeKI GCWGC 8 cut(s) 110, 395, 575, 656, 1763, 2228, 2845, 2876
ApoI RAATTY 6 cut(s) 423, 690, 2076, 2129, 2788, 2829
ArsI GACNNNNNNTTYG 2 cut(s) 1397, 1429
Asp700I GAANNNNTTC 1 cut(s) 2387
Asp718I GGTACC 2 cut(s) 1311, 1608
AspS9I GGNCC 2 cut(s) 1145, 2795
AsuC2I CCSGG 7 cut(s) 84, 85, 960, 1231, 1459, 2616, 2837
AsuHPI GGTGA 9 cut(s) 43, 185, 338, 1609, 1642, 1678, 1719, 1838, 1991
AvaI CYCGRG 2 cut(s) 83, 378
AvaII GGWCC 2 cut(s) 1145, 2795
AxyI CCTNAGG 1 cut(s) 2370
BaeGI GKGCMC 3 cut(s) 1585, 1914, 2584
BaeI ACNNNNGTAYC 2 cut(s) 1592, 1625
BalI TGGCCA 1 cut(s) 1556
BanI GGYRCC 6 cut(s) 1241, 1311, 1393, 1608, 2036, 2818
BarI GAAGNNNNNNTAC 2 cut(s) 1652, 1684
BauI CACGAG 1 cut(s) 2031
BbsI GAAGAC 2 cut(s) 380, 1804
Bbv12I GWGCWC 3 cut(s) 1578, 1585, 2221
BbvI GCAGC 8 cut(s) 97, 382, 562, 643, 1775, 2240, 2832, 2888
BccI CCATC 6 cut(s) 175, 550, 861, 1012, 2149, 2668
BceAI ACGGC 2 cut(s) 98, 2537
BcgI CGANNNNNNTGC 2 cut(s) 1663, 1697
BciT130I CCWGG 3 cut(s) 1320, 1737, 2306
BciVI GTATCC 1 cut(s) 2612
BclI TGATCA 2 cut(s) 1875, 3009
BcnI CCSGG 7 cut(s) 84, 85, 960, 1231, 1459, 2616, 2837
BcoDI GTCTC 1 cut(s) 251
BfaI CTAG 5 cut(s) 308, 1191, 1961, 2081, 2841
BfmI CTRYAG 3 cut(s) 680, 823, 2631
BfoI RGCGCY 5 cut(s) 387, 1245, 1784, 2285, 2517
BfuAI ACCTGC 3 cut(s) 422, 673, 2326
BfuI GTATCC 1 cut(s) 2612
BlpI GCTNAGC 1 cut(s) 714
BmcAI AGTACT 2 cut(s) 952, 2608
Bme18I GGWCC 2 cut(s) 1145, 2795
BmeT110I CYCGRG 2 cut(s) 83, 378
BmgT120I GGNCC 2 cut(s) 1145, 2795
BmsI GCATC 6 cut(s) 472, 594, 668, 1294, 1332, 2247
BpiI GAAGAC 2 cut(s) 380, 1804
BpmI CTGGAG 1 cut(s) 1302
Bpu10I CCTNAGC 1 cut(s) 142
Bpu1102I GCTNAGC 1 cut(s) 714
BpuMI CCSGG 7 cut(s) 84, 85, 960, 1231, 1459, 2616, 2837
Bsa29I ATCGAT 1 cut(s) 9
BsaAI YACGTR 1 cut(s) 1206
BsaHI GRCGYC 1 cut(s) 1242
BsaI GGTCTC 1 cut(s) 251
BsaJI CCNNGG 9 cut(s) 83, 747, 958, 1458, 1551, 1557, 2049, 2422, 2860
BsaWI WCCGGW 3 cut(s) 297, 1197, 1950
BsaXI ACNNNNNCTCC 8 cut(s) 79, 109, 1412, 1442, 1732, 1762, 3062, 3092
Bsc4I CCNNNNNNNGG 9 cut(s) 414, 966, 1230, 1325, 1736, 2067, 2523, 2622, 2674
Bse118I RCCGGY 2 cut(s) 1171, 2821
Bse1I ACTGG 7 cut(s) 596, 971, 1059, 1079, 1505, 2159, 2627
Bse21I CCTNAGG 1 cut(s) 2370
Bse3DI GCAATG 9 cut(s) 89, 390, 585, 813, 1344, 1742, 2106, 2469, 2901
BseBI CCWGG 3 cut(s) 1320, 1737, 2306
BseCI ATCGAT 1 cut(s) 9
BseDI CCNNGG 9 cut(s) 83, 747, 958, 1458, 1551, 1557, 2049, 2422, 2860
BseGI GGATG 2 cut(s) 659, 872
BseLI CCNNNNNNNGG 9 cut(s) 414, 966, 1230, 1325, 1736, 2067, 2523, 2622, 2674
BseMI GCAATG 9 cut(s) 89, 390, 585, 813, 1344, 1742, 2106, 2469, 2901
BseMII CTCAG 5 cut(s) 705, 1167, 1233, 1292, 1591
BseNI ACTGG 7 cut(s) 596, 971, 1059, 1079, 1505, 2159, 2627
BseRI GAGGAG 1 cut(s) 1436
BseSI GKGCMC 3 cut(s) 1585, 1914, 2584
BseX3I CGGCCG 2 cut(s) 46, 459
BseXI GCAGC 8 cut(s) 97, 382, 562, 643, 1775, 2240, 2832, 2888
BseYI CCCAGC 2 cut(s) 571, 1186
BsgI GTGCAG 1 cut(s) 967
Bsh1236I CGCG 6 cut(s) 464, 1226, 1658, 1898, 2051, 2248
Bsh1285I CGRYCG 3 cut(s) 49, 462, 864
BshFI GGCC 6 cut(s) 48, 150, 461, 1556, 1701, 2126
BshNI GGYRCC 6 cut(s) 1241, 1311, 1393, 1608, 2036, 2818
BshVI ATCGAT 1 cut(s) 9
BsiEI CGRYCG 3 cut(s) 49, 462, 864
BsiHKAI GWGCWC 3 cut(s) 1578, 1585, 2221
BsiHKCI CYCGRG 2 cut(s) 83, 378
BsiWI CGTACG 1 cut(s) 1659
BslFI GGGAC 3 cut(s) 100, 941, 2159
BslI CCNNNNNNNGG 9 cut(s) 414, 966, 1230, 1325, 1736, 2067, 2523, 2622, 2674
BsmAI GTCTC 1 cut(s) 251
BsmFI GGGAC 3 cut(s) 100, 941, 2159
BsmI GAATGC 6 cut(s) 649, 818, 898, 1256, 2554, 2906
BsnI GGCC 6 cut(s) 48, 150, 461, 1556, 1701, 2126
Bso31I GGTCTC 1 cut(s) 251
BsoBI CYCGRG 2 cut(s) 83, 378
Bsp1286I GDGCHC 5 cut(s) 1578, 1585, 1914, 2221, 2584
Bsp1720I GCTNAGC 1 cut(s) 714
Bsp19I CCATGG 3 cut(s) 1551, 2422, 2860
BspANI GGCC 6 cut(s) 48, 150, 461, 1556, 1701, 2126
BspCNI CTCAG 5 cut(s) 706, 1168, 1232, 1291, 1590
BspDI ATCGAT 1 cut(s) 9
BspFNI CGCG 6 cut(s) 464, 1226, 1658, 1898, 2051, 2248
BspHI TCATGA 3 cut(s) 1032, 1872, 2688
BspMAI CTGCAG 1 cut(s) 684
BspMI ACCTGC 3 cut(s) 422, 673, 2326
BspPI GGATC 2 cut(s) 1732, 2150
BspQI GCTCTTC 1 cut(s) 161
BspT107I GGYRCC 6 cut(s) 1241, 1311, 1393, 1608, 2036, 2818
BspTNI GGTCTC 1 cut(s) 251
BsrDI GCAATG 9 cut(s) 89, 390, 585, 813, 1344, 1742, 2106, 2469, 2901
BsrFI RCCGGY 2 cut(s) 1171, 2821
BsrI ACTGG 7 cut(s) 596, 971, 1059, 1079, 1505, 2159, 2627
BssAI RCCGGY 2 cut(s) 1171, 2821
BssECI CCNNGG 9 cut(s) 83, 747, 958, 1458, 1551, 1557, 2049, 2422, 2860
BssNI GRCGYC 1 cut(s) 1242
BssSI CACGAG 1 cut(s) 2031
BssT1I CCWWGG 5 cut(s) 747, 1551, 1557, 2422, 2860
Bst2BI CACGAG 1 cut(s) 2031
Bst2UI CCWGG 3 cut(s) 1320, 1737, 2306
Bst6I CTCTTC 4 cut(s) 20, 161, 185, 1673
BstACI GRCGYC 1 cut(s) 1242
BstAPI GCANNNNNTGC 1 cut(s) 644
BstBAI YACGTR 1 cut(s) 1206
BstC8I GCNNGC 6 cut(s) 148, 152, 167, 1173, 1820, 2823
BstDEI CTNAG 8 cut(s) 142, 714, 1176, 1219, 1278, 1577, 2370, 2403
BstDSI CCRYGG 4 cut(s) 1551, 2049, 2422, 2860
BstEII GGTNACC 1 cut(s) 31
BstF5I GGATG 2 cut(s) 659, 872
BstFNI CGCG 6 cut(s) 464, 1226, 1658, 1898, 2051, 2248
BstH2I RGCGCY 5 cut(s) 387, 1245, 1784, 2285, 2517
BstMAI GTCTC 1 cut(s) 251
BstMCI CGRYCG 3 cut(s) 49, 462, 864
BstNI CCWGG 3 cut(s) 1320, 1737, 2306
BstNSI RCATGY 2 cut(s) 670, 1574
BstPI GGTNACC 1 cut(s) 31
BstSFI CTRYAG 3 cut(s) 680, 823, 2631
BstSLI GKGCMC 3 cut(s) 1585, 1914, 2584
BstUI CGCG 6 cut(s) 464, 1226, 1658, 1898, 2051, 2248
BstV1I GCAGC 8 cut(s) 97, 382, 562, 643, 1775, 2240, 2832, 2888
BstV2I GAAGAC 2 cut(s) 380, 1804
BstZI CGGCCG 2 cut(s) 46, 459
Bsu15I ATCGAT 1 cut(s) 9
Bsu36I CCTNAGG 1 cut(s) 2370
BsuI GTATCC 1 cut(s) 2612
BsuRI GGCC 6 cut(s) 48, 150, 461, 1556, 1701, 2126
BsuTUI ATCGAT 1 cut(s) 9
BtgI CCRYGG 4 cut(s) 1551, 2049, 2422, 2860
BtsCI GGATG 2 cut(s) 659, 872
BtsI GCAGTG 2 cut(s) 1470, 1590
BveI ACCTGC 3 cut(s) 422, 673, 2326
Cac8I GCNNGC 6 cut(s) 148, 152, 167, 1173, 1820, 2823
CaiI CAGNNNCTG 2 cut(s) 1766, 2267
CciI TCATGA 3 cut(s) 1032, 1872, 2688
Cfr10I RCCGGY 2 cut(s) 1171, 2821
Cfr13I GGNCC 2 cut(s) 1145, 2795
Cfr42I CCGCGG 1 cut(s) 2052
Cfr9I CCCGGG 1 cut(s) 83
ClaI ATCGAT 1 cut(s) 9
CseI GACGC 2 cut(s) 1215, 1664
Csp6I GTAC 8 cut(s) 951, 1270, 1312, 1609, 1660, 2607, 2612, 3044
CspCI CAANNNNNGTGG 2 cut(s) 1147, 1182
CviQI GTAC 8 cut(s) 951, 1270, 1312, 1609, 1660, 2607, 2612, 3044
DdeI CTNAG 8 cut(s) 142, 714, 1176, 1219, 1278, 1577, 2370, 2403
DinI GGCGCC 1 cut(s) 1243
EaeI YGGCCR 4 cut(s) 46, 459, 1554, 1699
EagI CGGCCG 2 cut(s) 46, 459
Eam1104I CTCTTC 4 cut(s) 20, 161, 185, 1673
EarI CTCTTC 4 cut(s) 20, 161, 185, 1673
EciI GGCGGA 1 cut(s) 1984
EclXI CGGCCG 2 cut(s) 46, 459
Eco130I CCWWGG 5 cut(s) 747, 1551, 1557, 2422, 2860
Eco31I GGTCTC 1 cut(s) 251
Eco47I GGWCC 2 cut(s) 1145, 2795
Eco47III AGCGCT 1 cut(s) 2283
Eco52I CGGCCG 2 cut(s) 46, 459
Eco57I CTGAAG 1 cut(s) 225
Eco81I CCTNAGG 1 cut(s) 2370
Eco88I CYCGRG 2 cut(s) 83, 378
Eco91I GGTNACC 1 cut(s) 31
EcoO65I GGTNACC 1 cut(s) 31
EcoRI GAATTC 1 cut(s) 2076
EcoRII CCWGG 3 cut(s) 1318, 1735, 2304
EcoT14I CCWWGG 5 cut(s) 747, 1551, 1557, 2422, 2860
EcoT22I ATGCAT 2 cut(s) 6, 733
EgeI GGCGCC 1 cut(s) 1243
EheI GGCGCC 1 cut(s) 1243
ErhI CCWWGG 5 cut(s) 747, 1551, 1557, 2422, 2860
FaqI GGGAC 3 cut(s) 100, 941, 2159
FauNDI CATATG 3 cut(s) 227, 493, 1880
FbaI TGATCA 2 cut(s) 1875, 3009
FblI GTMKAC 2 cut(s) 1202, 2852
FokI GGATG 2 cut(s) 646, 879
FspBI CTAG 5 cut(s) 308, 1191, 1961, 2081, 2841
GsaI CCCAGC 2 cut(s) 575, 1190
GsuI CTGGAG 1 cut(s) 1302
HaeII RGCGCY 5 cut(s) 387, 1245, 1784, 2285, 2517
HaeIII GGCC 6 cut(s) 48, 150, 461, 1556, 1701, 2126
HgaI GACGC 2 cut(s) 1215, 1664
Hin1I GRCGYC 1 cut(s) 1242
HincII GTYRAC 5 cut(s) 67, 282, 1363, 2302, 2341
HindII GTYRAC 5 cut(s) 67, 282, 1363, 2302, 2341
HindIII AAGCTT 1 cut(s) 1235
HphI GGTGA 9 cut(s) 43, 185, 338, 1609, 1642, 1678, 1719, 1838, 1991
Hpy188III TCNNGA 4 cut(s) 1033, 1873, 2689, 2983
Hpy99I CGWCG 1 cut(s) 1658
HpyAV CCTTC 6 cut(s) 180, 703, 1135, 1715, 2494, 2647
HpyCH4IV ACGT 4 cut(s) 63, 1205, 1716, 3035
HpyF3I CTNAG 8 cut(s) 142, 714, 1176, 1219, 1278, 1577, 2370, 2403
HpySE526I ACGT 4 cut(s) 63, 1205, 1716, 3035
Hsp92I GRCGYC 1 cut(s) 1242
KasI GGCGCC 1 cut(s) 1241
KpnI GGTACC 2 cut(s) 1315, 1612
KroI GCCGGC 2 cut(s) 1171, 2821
KroNI GCCGGC 2 cut(s) 1173, 2823
Ksp22I TGATCA 2 cut(s) 1875, 3009
KspI CCGCGG 1 cut(s) 2052
LguI GCTCTTC 1 cut(s) 161
LmnI GCTCC 6 cut(s) 490, 563, 1474, 1889, 2143, 2216
Lsp1109I GCAGC 8 cut(s) 97, 382, 562, 643, 1775, 2240, 2832, 2888
LweI GCATC 6 cut(s) 472, 594, 668, 1294, 1332, 2247
MaeI CTAG 5 cut(s) 308, 1191, 1961, 2081, 2841
MaeII ACGT 4 cut(s) 63, 1205, 1716, 3035
MaeIII GTNAC 8 cut(s) 31, 173, 326, 620, 1684, 1826, 1979, 2273
MfeI CAATTG 1 cut(s) 1623
MhlI GDGCHC 5 cut(s) 1578, 1585, 1914, 2221, 2584
MlsI TGGCCA 1 cut(s) 1556
MluI ACGCGT 1 cut(s) 1656
MluNI TGGCCA 1 cut(s) 1556
Mly113I GGCGCC 1 cut(s) 1242
MlyI GAGTC 4 cut(s) 202, 370, 1072, 2023
MmeI TCCRAC 6 cut(s) 512, 687, 1398, 1452, 2194, 2504
Mox20I TGGCCA 1 cut(s) 1556
Mph1103I ATGCAT 2 cut(s) 6, 733
MroNI GCCGGC 2 cut(s) 1171, 2821
MroXI GAANNNNTTC 1 cut(s) 2387
MscI TGGCCA 1 cut(s) 1556
MseI TTAA 2 cut(s) 468, 1332
MslI CAYNNNNRTG 8 cut(s) 350, 602, 765, 1037, 2003, 2207, 2421, 2693
Msp20I TGGCCA 1 cut(s) 1556
MspA1I CMGCKG 4 cut(s) 110, 575, 1766, 2051
MunI CAATTG 1 cut(s) 1623
Mva1269I GAATGC 6 cut(s) 649, 818, 898, 1256, 2554, 2906
MvaI CCWGG 3 cut(s) 1320, 1737, 2306
MvnI CGCG 6 cut(s) 464, 1226, 1658, 1898, 2051, 2248
NaeI GCCGGC 2 cut(s) 1173, 2823
NarI GGCGCC 1 cut(s) 1242
NciI CCSGG 7 cut(s) 84, 85, 960, 1231, 1459, 2616, 2837
NcoI CCATGG 3 cut(s) 1551, 2422, 2860
NdeI CATATG 3 cut(s) 227, 493, 1880
NgoMIV GCCGGC 2 cut(s) 1171, 2821
NmeAIII GCCGAG 1 cut(s) 24
NmuCI GTSAC 6 cut(s) 31, 173, 326, 1684, 1826, 1979
NsiI ATGCAT 2 cut(s) 6, 733
NspI RCATGY 2 cut(s) 670, 1574
PagI TCATGA 3 cut(s) 1032, 1872, 2688
PaqCI CACCTGC 1 cut(s) 422
PciI ACATGT 1 cut(s) 666
PciSI GCTCTTC 1 cut(s) 161
PctI GAATGC 6 cut(s) 649, 818, 898, 1256, 2554, 2906
PdiI GCCGGC 2 cut(s) 1173, 2823
PdmI GAANNNNTTC 1 cut(s) 2387
Pfl23II CGTACG 1 cut(s) 1659
PflMI CCANNNNNTGG 2 cut(s) 2523, 2674
PfoI TCCNGGA 1 cut(s) 1229
Ple19I CGATCG 1 cut(s) 864
PleI GAGTC 4 cut(s) 202, 370, 1072, 2023
PluTI GGCGCC 1 cut(s) 1245
PpsI GAGTC 4 cut(s) 202, 370, 1072, 2023
Ppu21I YACGTR 1 cut(s) 1206
PscI ACATGT 1 cut(s) 666
Psp6I CCWGG 3 cut(s) 1318, 1735, 2304
PspEI GGTNACC 1 cut(s) 31
PspFI CCCAGC 2 cut(s) 571, 1186
PspGI CCWGG 3 cut(s) 1318, 1735, 2304
PspLI CGTACG 1 cut(s) 1659
PspPI GGNCC 2 cut(s) 1145, 2795
PstI CTGCAG 1 cut(s) 684
PstNI CAGNNNCTG 2 cut(s) 1766, 2267
PvuI CGATCG 1 cut(s) 864
PvuII CAGCTG 3 cut(s) 110, 575, 1766
RsaI GTAC 8 cut(s) 952, 1271, 1313, 1610, 1661, 2608, 2613, 3045
RsaNI GTAC 8 cut(s) 951, 1270, 1312, 1609, 1660, 2607, 2612, 3044
RseI CAYNNNNRTG 8 cut(s) 350, 602, 765, 1037, 2003, 2207, 2421, 2693
SacII CCGCGG 1 cut(s) 2052
SapI GCTCTTC 1 cut(s) 161
SaqAI TTAA 2 cut(s) 468, 1332
Sau96I GGNCC 2 cut(s) 1145, 2795
ScaI AGTACT 2 cut(s) 952, 2608
SchI GAGTC 4 cut(s) 202, 370, 1072, 2023
SduI GDGCHC 5 cut(s) 1578, 1585, 1914, 2221, 2584
SfaNI GCATC 6 cut(s) 472, 594, 668, 1294, 1332, 2247
SfcI CTRYAG 3 cut(s) 680, 823, 2631
SfoI GGCGCC 1 cut(s) 1243
Sfr303I CCGCGG 1 cut(s) 2052
SgrBI CCGCGG 1 cut(s) 2052
SinI GGWCC 2 cut(s) 1145, 2795
SmaI CCCGGG 1 cut(s) 85
SmiMI CAYNNNNRTG 8 cut(s) 350, 602, 765, 1037, 2003, 2207, 2421, 2693
SspDI GGCGCC 1 cut(s) 1241
SspMI CTAG 5 cut(s) 308, 1191, 1961, 2081, 2841
StyI CCWWGG 5 cut(s) 747, 1551, 1557, 2422, 2860
TaiI ACGT 4 cut(s) 66, 1208, 1719, 3038
TaqI TCGA 4 cut(s) 9, 1403, 2121, 3053
TaqII GACCGA 1 cut(s) 1133
TatI WGTACW 3 cut(s) 950, 1269, 2606
TauI GCSGC 6 cut(s) 51, 464, 1226, 1704, 2051, 2876
Tru1I TTAA 2 cut(s) 468, 1332
Tru9I TTAA 2 cut(s) 468, 1332
TseFI GTSAC 6 cut(s) 31, 173, 326, 1684, 1826, 1979
TseI GCWGC 8 cut(s) 110, 395, 575, 656, 1763, 2228, 2845, 2876
Tsp45I GTSAC 6 cut(s) 31, 173, 326, 1684, 1826, 1979
TspMI CCCGGG 1 cut(s) 83
Van91I CCANNNNNTGG 2 cut(s) 2523, 2674
VneI GTGCAC 1 cut(s) 1581
VpaK11BI GGWCC 2 cut(s) 1145, 2795
XapI RAATTY 6 cut(s) 423, 690, 2076, 2129, 2788, 2829
XceI RCATGY 2 cut(s) 670, 1574
XcmI CCANNNNNNNNNTGG 1 cut(s) 158
XmaI CCCGGG 1 cut(s) 83
XmiI GTMKAC 2 cut(s) 1202, 2852
XmnI GAANNNNTTC 1 cut(s) 2387
XspI CTAG 5 cut(s) 308, 1191, 1961, 2081, 2841
ZrmI AGTACT 2 cut(s) 952, 2608
Zsp2I ATGCAT 2 cut(s) 6, 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.