pycom05g28750

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0029287
Physical Location & Seq
Reverse (-)
29352494 .. 29353674
1181 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 792 bp
ATGATGGAAATTCAGAAATTAACGGTTAATTATTGCTCAGAAGAGAAATTGCAACTGAAAATTGATATCGAAAAAAAAAAAAGTCACAACAACAGATTGAAACACGGTGGAGTTTCGCCGCTGGAAAAAAAAAAGATATTGATCCGATCAACTACCGAGCTCCGGTGGTGGAGGAAGGTTGAGATCGAACCCTCCGCCGCCGCGAGGAAGATCGTTCTGGTTCAGATCGACCACCGAGGACGAGTCCGAGTCGCTCTGGGCCGTACCGGCAGCGTGGAAATCGGGCTCGTGGAAGTTGGGGTGATCGAATCTCATGCGTGGATGAAACTGGCGGTTCTTCATCACGCTGGCTCTGAAAACCGAGTCAAAATAGAGCACCTGATTCGCGGCCGCCGGGGCGGAAGACGGAACTCCGATGACAGCTCCGAATCCTGGAACCTGGTGGTGCTGGAAGGGGAACCTCATCGGCGCGGAGCCGAATCCGCCGGAGCTGACACCGCCCGGGCGGCTTCCTCCGCCGTGTCGAAGGTTCCGAGCCAGACCCGGCTCTTCTTGCCGGGATCTCTGATCTCGGCGGCGTACCTCCCCCACGGCCTCTTCCTCACGCCTCTAAAATGCACCTCCTTCACGTTTCCGTTACCGTTCATCCTAACGGCTGCGGCGGCCGTCTTCTCTCTCGGCGCCATAACTAAACGGTGGCGTTTTCTGTTTGTAGTGTTTGGATGGAAAAGAGTGTTTGGCTGCAGAAAATATGTGAGAGAAAGGGGTGTGGAGATGGCGATGGAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

264

Amino Acids

29.56

Weight (kDa)

10.22

Isoelectric Point (pI)

53.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029287)

Species Orthologous Gene IDs
pyrus_communis pycom05g28750 pycom15g17190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 680
AccII CGCG 3 cut(s) 203, 387, 471
AclWI GGATC 2 cut(s) 136, 568
AcoI YGGCCR 2 cut(s) 388, 663
AcsI RAATTY 1 cut(s) 9
AcyI GRCGYC 1 cut(s) 681
AfaI GTAC 2 cut(s) 265, 581
AfiI CCNNNNNNNGG 3 cut(s) 162, 204, 432
AgsI TTSAA 1 cut(s) 100
AjnI CCWGG 2 cut(s) 431, 438
AluBI AGCT 3 cut(s) 160, 423, 491
AluI AGCT 3 cut(s) 160, 423, 491
Alw21I GWGCWC 2 cut(s) 162, 378
AlwI GGATC 2 cut(s) 136, 568
Ama87I CYCGRG 1 cut(s) 501
AoxI GGCC 4 cut(s) 259, 388, 592, 663
ApeKI GCWGC 3 cut(s) 270, 656, 741
ApoI RAATTY 1 cut(s) 9
AspLEI GCGC 2 cut(s) 471, 683
AspS9I GGNCC 1 cut(s) 259
AsuC2I CCSGG 5 cut(s) 395, 502, 503, 544, 558
AsuHPI GGTGA 1 cut(s) 313
AvaI CYCGRG 1 cut(s) 501
BanI GGYRCC 1 cut(s) 680
BanII GRGCYC 2 cut(s) 162, 288
BauI CACGAG 1 cut(s) 287
BbsI GAAGAC 2 cut(s) 409, 661
Bbv12I GWGCWC 2 cut(s) 162, 378
BbvI GCAGC 3 cut(s) 282, 643, 728
BccI CCATC 3 cut(s) 717, 769, 775
BceAI ACGGC 5 cut(s) 246, 503, 607, 650, 669
BcgI CGANNNNNNTGC 4 cut(s) 296, 330, 365, 399
BciT130I CCWGG 2 cut(s) 433, 440
BcnI CCSGG 5 cut(s) 395, 502, 503, 544, 558
BfmI CTRYAG 1 cut(s) 742
BfoI RGCGCY 1 cut(s) 684
BglI GCCNNNNNGGC 3 cut(s) 267, 396, 506
Bme1390I CCNGG 7 cut(s) 395, 433, 440, 502, 503, 544, 558
BmeT110I CYCGRG 1 cut(s) 501
BmgT120I GGNCC 1 cut(s) 259
BmiI GGNNCC 5 cut(s) 437, 459, 475, 531, 682
BmrFI CCNGG 7 cut(s) 395, 433, 440, 502, 503, 544, 558
BpiI GAAGAC 2 cut(s) 409, 661
BpuMI CCSGG 5 cut(s) 395, 502, 503, 544, 558
BsaBI GATNNNNATC 1 cut(s) 140
BsaHI GRCGYC 1 cut(s) 681
BsaJI CCNNGG 4 cut(s) 235, 394, 501, 589
BsaWI WCCGGW 1 cut(s) 162
Bsc4I CCNNNNNNNGG 3 cut(s) 162, 204, 432
Bse118I RCCGGY 1 cut(s) 266
Bse1I ACTGG 1 cut(s) 333
Bse8I GATNNNNATC 1 cut(s) 140
BseBI CCWGG 2 cut(s) 433, 440
BseDI CCNNGG 4 cut(s) 235, 394, 501, 589
BseGI GGATG 3 cut(s) 327, 645, 728
BseJI GATNNNNATC 1 cut(s) 140
BseLI CCNNNNNNNGG 3 cut(s) 162, 204, 432
BseMII CTCAG 1 cut(s) 51
BseNI ACTGG 1 cut(s) 333
BseX3I CGGCCG 2 cut(s) 388, 663
BseXI GCAGC 3 cut(s) 282, 643, 728
Bsh1236I CGCG 3 cut(s) 203, 387, 471
Bsh1285I CGRYCG 2 cut(s) 391, 666
BshFI GGCC 4 cut(s) 261, 390, 594, 665
BshNI GGYRCC 1 cut(s) 680
BsiEI CGRYCG 2 cut(s) 391, 666
BsiHKAI GWGCWC 2 cut(s) 162, 378
BsiHKCI CYCGRG 1 cut(s) 501
BsiSI CCGG 7 cut(s) 163, 267, 394, 486, 502, 544, 557
BslI CCNNNNNNNGG 3 cut(s) 162, 204, 432
BsnI GGCC 4 cut(s) 261, 390, 594, 665
BsoBI CYCGRG 1 cut(s) 501
Bsp1286I GDGCHC 3 cut(s) 162, 288, 378
Bsp143I GATC 8 cut(s) 141, 146, 183, 210, 225, 303, 560, 567
BspANI GGCC 4 cut(s) 261, 390, 594, 665
BspCNI CTCAG 1 cut(s) 50
BspFNI CGCG 3 cut(s) 203, 387, 471
BspLI GGNNCC 5 cut(s) 437, 459, 475, 531, 682
BspMAI CTGCAG 1 cut(s) 746
BspPI GGATC 2 cut(s) 136, 568
BspQI GCTCTTC 1 cut(s) 554
BspT107I GGYRCC 1 cut(s) 680
BsrFI RCCGGY 1 cut(s) 266
BsrI ACTGG 1 cut(s) 333
BssAI RCCGGY 1 cut(s) 266
BssECI CCNNGG 4 cut(s) 235, 394, 501, 589
BssMI GATC 8 cut(s) 141, 146, 183, 210, 225, 303, 560, 567
BssNI GRCGYC 1 cut(s) 681
BssSI CACGAG 1 cut(s) 287
Bst2BI CACGAG 1 cut(s) 287
Bst2UI CCWGG 2 cut(s) 433, 440
Bst4CI ACNGT 4 cut(s) 25, 107, 642, 696
Bst6I CTCTTC 3 cut(s) 36, 554, 602
BstACI GRCGYC 1 cut(s) 681
BstC8I GCNNGC 1 cut(s) 349
BstDEI CTNAG 1 cut(s) 37
BstDSI CCRYGG 1 cut(s) 589
BstF5I GGATG 3 cut(s) 327, 645, 728
BstFNI CGCG 3 cut(s) 203, 387, 471
BstH2I RGCGCY 1 cut(s) 684
BstHHI GCGC 2 cut(s) 471, 683
BstKTI GATC 8 cut(s) 144, 149, 186, 213, 228, 306, 563, 570
BstMBI GATC 8 cut(s) 141, 146, 183, 210, 225, 303, 560, 567
BstMCI CGRYCG 2 cut(s) 391, 666
BstMWI GCNNNNNNNGC 8 cut(s) 267, 396, 482, 497, 506, 515, 553, 662
BstNI CCWGG 2 cut(s) 433, 440
BstSCI CCNGG 7 cut(s) 393, 431, 438, 500, 501, 542, 556
BstSFI CTRYAG 1 cut(s) 742
BstUI CGCG 3 cut(s) 203, 387, 471
BstV1I GCAGC 3 cut(s) 282, 643, 728
BstV2I GAAGAC 2 cut(s) 409, 661
BstX2I RGATCY 1 cut(s) 560
BstYI RGATCY 1 cut(s) 560
BstZI CGGCCG 2 cut(s) 388, 663
BsuRI GGCC 4 cut(s) 261, 390, 594, 665
BtgI CCRYGG 1 cut(s) 589
BtsCI GGATG 3 cut(s) 327, 645, 728
Cac8I GCNNGC 1 cut(s) 349
CciNI GCGGCCGC 1 cut(s) 388
CfoI GCGC 2 cut(s) 471, 683
Cfr10I RCCGGY 1 cut(s) 266
Cfr13I GGNCC 1 cut(s) 259
Cfr9I CCCGGG 1 cut(s) 501
CsiI ACCWGGT 1 cut(s) 438
Csp6I GTAC 2 cut(s) 264, 580
CviAII CATG 1 cut(s) 314
CviQI GTAC 2 cut(s) 264, 580
DdeI CTNAG 1 cut(s) 37
DinI GGCGCC 1 cut(s) 682
DpnI GATC 8 cut(s) 143, 148, 185, 212, 227, 305, 562, 569
DpnII GATC 8 cut(s) 141, 146, 183, 210, 225, 303, 560, 567
EaeI YGGCCR 2 cut(s) 388, 663
EagI CGGCCG 2 cut(s) 388, 663
Eam1104I CTCTTC 3 cut(s) 36, 554, 602
EarI CTCTTC 3 cut(s) 36, 554, 602
EciI GGCGGA 4 cut(s) 184, 414, 472, 505
Ecl136II GAGCTC 1 cut(s) 160
EclXI CGGCCG 2 cut(s) 388, 663
Eco24I GRGCYC 2 cut(s) 162, 288
Eco32I GATATC 1 cut(s) 67
Eco52I CGGCCG 2 cut(s) 388, 663
Eco53kI GAGCTC 1 cut(s) 160
Eco88I CYCGRG 1 cut(s) 501
EcoICRI GAGCTC 1 cut(s) 160
EcoRII CCWGG 2 cut(s) 431, 438
EcoRV GATATC 1 cut(s) 67
EcoT38I GRGCYC 2 cut(s) 162, 288
EgeI GGCGCC 1 cut(s) 682
EheI GGCGCC 1 cut(s) 682
FaeI CATG 1 cut(s) 317
FaiI YATR 3 cut(s) 315, 686, 753
FatI CATG 1 cut(s) 313
FokI GGATG 3 cut(s) 334, 632, 735
FriOI GRGCYC 2 cut(s) 162, 288
GlaI GCGC 2 cut(s) 470, 682
HaeII RGCGCY 1 cut(s) 684
HaeIII GGCC 4 cut(s) 261, 390, 594, 665
HapII CCGG 7 cut(s) 163, 267, 394, 486, 502, 544, 557
HhaI GCGC 2 cut(s) 471, 683
Hin1I GRCGYC 1 cut(s) 681
Hin1II CATG 1 cut(s) 317
Hin6I GCGC 2 cut(s) 469, 681
HinP1I GCGC 2 cut(s) 469, 681
HinfI GANTC 7 cut(s) 243, 249, 308, 363, 382, 428, 479
HpaII CCGG 7 cut(s) 163, 267, 394, 486, 502, 544, 557
HphI GGTGA 1 cut(s) 313
HpyAV CCTTC 4 cut(s) 169, 446, 520, 634
HpyCH4III ACNGT 4 cut(s) 25, 107, 642, 696
HpyCH4IV ACGT 1 cut(s) 629
HpyCH4V TGCA 3 cut(s) 52, 618, 744
HpyF10VI GCNNNNNNNGC 8 cut(s) 267, 396, 482, 497, 506, 515, 553, 662
HpyF3I CTNAG 1 cut(s) 37
HpySE526I ACGT 1 cut(s) 629
Hsp92I GRCGYC 1 cut(s) 681
Hsp92II CATG 1 cut(s) 317
HspAI GCGC 2 cut(s) 469, 681
KasI GGCGCC 1 cut(s) 680
Kzo9I GATC 8 cut(s) 141, 146, 183, 210, 225, 303, 560, 567
LguI GCTCTTC 1 cut(s) 554
LmnI GCTCC 4 cut(s) 165, 428, 473, 488
Lsp1109I GCAGC 3 cut(s) 282, 643, 728
MabI ACCWGGT 1 cut(s) 438
MaeII ACGT 1 cut(s) 629
MaeIII GTNAC 2 cut(s) 83, 636
MalI GATC 8 cut(s) 143, 148, 185, 212, 227, 305, 562, 569
MboI GATC 8 cut(s) 141, 146, 183, 210, 225, 303, 560, 567
MboII GAAGA 7 cut(s) 53, 220, 329, 414, 541, 589, 661
MflI RGATCY 1 cut(s) 560
MhlI GDGCHC 3 cut(s) 162, 288, 378
MluCI AATT 5 cut(s) 9, 17, 28, 47, 60
Mly113I GGCGCC 1 cut(s) 681
MlyI GAGTC 3 cut(s) 252, 258, 372
MseI TTAA 2 cut(s) 20, 27
MspA1I CMGCKG 1 cut(s) 121
MspI CCGG 7 cut(s) 163, 267, 394, 486, 502, 544, 557
MspR9I CCNGG 7 cut(s) 395, 433, 440, 502, 503, 544, 558
MvaI CCWGG 2 cut(s) 433, 440
MvnI CGCG 3 cut(s) 203, 387, 471
MwoI GCNNNNNNNGC 8 cut(s) 267, 396, 482, 497, 506, 515, 553, 662
NarI GGCGCC 1 cut(s) 681
NciI CCSGG 5 cut(s) 395, 502, 503, 544, 558
NdeII GATC 8 cut(s) 141, 146, 183, 210, 225, 303, 560, 567
NlaIII CATG 1 cut(s) 317
NlaIV GGNNCC 5 cut(s) 437, 459, 475, 531, 682
NmeAIII GCCGAG 2 cut(s) 551, 657
NmuCI GTSAC 1 cut(s) 83
NotI GCGGCCGC 1 cut(s) 388
PciSI GCTCTTC 1 cut(s) 554
PcsI WCGNNNNNNNCGW 1 cut(s) 530
PfeI GAWTC 4 cut(s) 308, 382, 428, 479
PfoI TCCNGGA 1 cut(s) 431
PleI GAGTC 3 cut(s) 251, 257, 371
PluTI GGCGCC 1 cut(s) 684
PpsI GAGTC 3 cut(s) 251, 257, 371
Psp124BI GAGCTC 1 cut(s) 162
Psp6I CCWGG 2 cut(s) 431, 438
PspGI CCWGG 2 cut(s) 431, 438
PspN4I GGNNCC 5 cut(s) 437, 459, 475, 531, 682
PspPI GGNCC 1 cut(s) 259
PstI CTGCAG 1 cut(s) 746
PsuI RGATCY 1 cut(s) 560
RsaI GTAC 2 cut(s) 265, 581
RsaNI GTAC 2 cut(s) 264, 580
SacI GAGCTC 1 cut(s) 162
SapI GCTCTTC 1 cut(s) 554
SaqAI TTAA 2 cut(s) 20, 27
Sau3AI GATC 8 cut(s) 141, 146, 183, 210, 225, 303, 560, 567
Sau96I GGNCC 1 cut(s) 259
SchI GAGTC 3 cut(s) 252, 258, 372
ScrFI CCNGG 7 cut(s) 395, 433, 440, 502, 503, 544, 558
SduI GDGCHC 3 cut(s) 162, 288, 378
SexAI ACCWGGT 1 cut(s) 438
SfcI CTRYAG 1 cut(s) 742
SfoI GGCGCC 1 cut(s) 682
SmaI CCCGGG 1 cut(s) 503
SrfI GCCCGGGC 1 cut(s) 503
Sse9I AATT 5 cut(s) 9, 17, 28, 47, 60
SspDI GGCGCC 1 cut(s) 680
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 7 cut(s) 393, 431, 438, 500, 501, 542, 556
TaaI ACNGT 4 cut(s) 25, 107, 642, 696
TaiI ACGT 1 cut(s) 632
TaqI TCGA 5 cut(s) 69, 186, 228, 306, 524
TasI AATT 5 cut(s) 9, 17, 28, 47, 60
TauI GCSGC 9 cut(s) 121, 200, 203, 390, 393, 509, 578, 662, 665
TfiI GAWTC 4 cut(s) 308, 382, 428, 479
Tru1I TTAA 2 cut(s) 20, 27
Tru9I TTAA 2 cut(s) 20, 27
TseFI GTSAC 1 cut(s) 83
TseI GCWGC 3 cut(s) 270, 656, 741
Tsp45I GTSAC 1 cut(s) 83
TspDTI ATGAA 3 cut(s) 329, 338, 634
TspGWI ACGGA 2 cut(s) 421, 624
TspMI CCCGGG 1 cut(s) 501
XapI RAATTY 1 cut(s) 9
XmaI CCCGGG 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.