pycom05g31220

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0029288
Physical Location & Seq
Forward (+)
31058813 .. 31060174
1362 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g31220.1

Sequence Viewer

Length: 786 bp
ATGTGTGCCGATGAGGATGGGGATCTGTTAATAGAGTCCGCAGTTAAAAACGTTCCCAAAGTTTTTGTTGTATCAACTTTGGTGAGCGTGATGCCAGCTCAGCCTTTCTTATTCCGAAATTATCAGTATCCTGCGGGAACACTAGAAGTGCCTCTTGCAATTTCAGAAAGTTCAGGAATAACTGGACCACCTCCTGGATGTGCTGAAGGTGGCTATAAGCACAGTGCCTTTATAGGAAGTTGTAAGCATCAAGTATGGCAAGCTATAAGAGCGTCATCTGCTGCTCCGTATTATCTTGATGATTTCTCAGATGATGTAAATCGTTGGCAAGATGGTGCAATAGTGGCAAACAATCCTACAATCTTTTCCATTAGAGAAGCACAACTTTTATGGCCAGACACAAAAATTGACTGCTTAGTTTCCGTTGGCTGTGGTGATGTTCCAACAAAGGTGCGAAAAGGTGGTTGGCGTTATCTGGATACTGGTCAGGTATTGATAGAAAGTGCATGCTCTGTGGAACGGGTGGAGGAAGCTTTGAGCACATTGCTACCCATGCTCCCTGGAATACAATATTTTCGGTTTAATCCAGTTGATGAACGCTGTGATATGGAACTGGATGAGACTGATCCTGCACTCTGGCTGAAATTGGAAGATGCAGTTGAGGAATATATTCAGAAAAATTCTCTTGTACTTAAGGATGCCTGTGAGAGACTACTTATGCCCTTTCAACATGATGAGAAGTGGTCACATTTTTTACTCTTTTTTCTAGAATGTAAGCTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001516 GO:0001676 GO:0003674 GO:0003824 GO:0004620 GO:0004623 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006636 GO:0006644 GO:0006650 GO:0006690 GO:0006692 GO:0006693 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008219 GO:0008610 GO:0009056 GO:0009058 GO:0009395 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009694 GO:0009695 GO:0009987 GO:0010817 GO:0010876 GO:0015711 GO:0015718 GO:0015849 GO:0015908 GO:0015909 GO:0016020 GO:0016042 GO:0016053 GO:0016298 GO:0016787 GO:0016788 GO:0019369 GO:0019637 GO:0019752 GO:0031090 GO:0031903 GO:0032309 GO:0032787 GO:0033036 GO:0033559 GO:0034638 GO:0042445 GO:0042446 GO:0042579 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043651 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0046337 GO:0046338 GO:0046394 GO:0046434 GO:0046456 GO:0046457 GO:0046470 GO:0046475 GO:0046486 GO:0046503 GO:0046717 GO:0046903 GO:0046942 GO:0047372 GO:0047499 GO:0050482 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065008 GO:0071702 GO:0071704 GO:0071715 GO:0072330 GO:0097164 GO:0098542 GO:0098588 GO:0098805 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901571 GO:1901575 GO:1901576 GO:1903963
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm LRR_8 Patatin

Protein Analysis

262

Amino Acids

29.21

Weight (kDa)

4.6

Isoelectric Point (pI)

41.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 83 - 120 4.8e-06 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0029288)

Species Orthologous Gene IDs
pyrus_communis pycom05g31220 pycom10g26920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 194
AciI CCGC 2 cut(s) 39, 134
AclI AACGTT 1 cut(s) 51
AclWI GGATC 2 cut(s) 30, 620
AcoI YGGCCR 1 cut(s) 392
AcsI RAATTY 1 cut(s) 679
AcuI CTGAAG 1 cut(s) 225
AfaI GTAC 1 cut(s) 690
AfiI CCNNNNNNNGG 1 cut(s) 194
AflII CTTAAG 1 cut(s) 692
AgsI TTSAA 1 cut(s) 728
AjnI CCWGG 2 cut(s) 193, 559
AjuI GAANNNNNNNTTGG 2 cut(s) 448, 480
AluBI AGCT 4 cut(s) 98, 263, 533, 778
AluI AGCT 4 cut(s) 98, 263, 533, 778
Alw21I GWGCWC 1 cut(s) 542
Alw26I GTCTC 2 cut(s) 614, 703
AlwI GGATC 2 cut(s) 30, 620
AoxI GGCC 1 cut(s) 392
ApeKI GCWGC 1 cut(s) 281
ApoI RAATTY 1 cut(s) 679
Asp700I GAANNNNTTC 1 cut(s) 669
AspS9I GGNCC 1 cut(s) 185
AsuHPI GGTGA 2 cut(s) 94, 446
AvaII GGWCC 1 cut(s) 185
BalI TGGCCA 1 cut(s) 394
Bbv12I GWGCWC 1 cut(s) 542
BbvI GCAGC 1 cut(s) 268
BccI CCATC 2 cut(s) 11, 326
BciT130I CCWGG 2 cut(s) 195, 561
BciVI GTATCC 2 cut(s) 138, 472
BcoDI GTCTC 2 cut(s) 614, 703
BfaI CTAG 2 cut(s) 143, 767
BfrI CTTAAG 1 cut(s) 692
BfuI GTATCC 2 cut(s) 138, 472
BisI GCNGC 1 cut(s) 282
BlpI GCTNAGC 1 cut(s) 99
BlsI GCNGC 1 cut(s) 283
Bme1390I CCNGG 2 cut(s) 195, 561
Bme18I GGWCC 1 cut(s) 185
BmgT120I GGNCC 1 cut(s) 185
BmrFI CCNGG 2 cut(s) 195, 561
BmsI GCATC 4 cut(s) 81, 256, 643, 688
Bpu1102I GCTNAGC 1 cut(s) 99
BsaBI GATNNNNATC 2 cut(s) 21, 318
BsaJI CCNNGG 1 cut(s) 559
BsaXI ACNNNNNCTCC 2 cut(s) 540, 570
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse1I ACTGG 4 cut(s) 187, 487, 587, 618
Bse3DI GCAATG 1 cut(s) 542
Bse8I GATNNNNATC 2 cut(s) 21, 318
BseBI CCWGG 2 cut(s) 195, 561
BseDI CCNNGG 1 cut(s) 559
BseGI GGATG 4 cut(s) 22, 203, 622, 703
BseJI GATNNNNATC 2 cut(s) 21, 318
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMI GCAATG 1 cut(s) 542
BseMII CTCAG 2 cut(s) 113, 321
BseNI ACTGG 4 cut(s) 187, 487, 587, 618
BseXI GCAGC 1 cut(s) 268
BsgI GTGCAG 1 cut(s) 615
BshFI GGCC 1 cut(s) 394
BsiHKAI GWGCWC 1 cut(s) 542
BslI CCNNNNNNNGG 1 cut(s) 194
BsmAI GTCTC 2 cut(s) 614, 703
BsnI GGCC 1 cut(s) 394
Bsp1286I GDGCHC 1 cut(s) 542
Bsp143I GATC 2 cut(s) 22, 625
Bsp1720I GCTNAGC 1 cut(s) 99
BspACI CCGC 2 cut(s) 39, 134
BspANI GGCC 1 cut(s) 394
BspCNI CTCAG 2 cut(s) 112, 320
BspPI GGATC 2 cut(s) 30, 620
BspTI CTTAAG 1 cut(s) 692
BsrDI GCAATG 1 cut(s) 542
BsrI ACTGG 4 cut(s) 187, 487, 587, 618
BssECI CCNNGG 1 cut(s) 559
BssMI GATC 2 cut(s) 22, 625
Bst2UI CCWGG 2 cut(s) 195, 561
Bst4CI ACNGT 1 cut(s) 224
BstAFI CTTAAG 1 cut(s) 692
BstC8I GCNNGC 3 cut(s) 96, 261, 508
BstDEI CTNAG 3 cut(s) 99, 307, 415
BstF5I GGATG 4 cut(s) 22, 203, 622, 703
BstKTI GATC 2 cut(s) 25, 628
BstMAI GTCTC 2 cut(s) 614, 703
BstMBI GATC 2 cut(s) 22, 625
BstMWI GCNNNNNNNGC 5 cut(s) 100, 269, 278, 344, 553
BstNI CCWGG 2 cut(s) 195, 561
BstNSI RCATGY 1 cut(s) 510
BstSCI CCNGG 2 cut(s) 193, 559
BstV1I GCAGC 1 cut(s) 268
BstX2I RGATCY 1 cut(s) 22
BstYI RGATCY 1 cut(s) 22
BsuI GTATCC 2 cut(s) 138, 472
BsuRI GGCC 1 cut(s) 394
BtsCI GGATG 4 cut(s) 22, 203, 622, 703
BtsIMutI CAGTG 1 cut(s) 229
Cac8I GCNNGC 3 cut(s) 96, 261, 508
Cfr13I GGNCC 1 cut(s) 185
CseI GACGC 1 cut(s) 261
Csp6I GTAC 1 cut(s) 689
CviAII CATG 3 cut(s) 507, 553, 731
CviJI RGCY 9 cut(s) 98, 103, 213, 263, 394, 429, 533, 640, 778
CviKI_1 RGCY 9 cut(s) 98, 103, 213, 263, 394, 429, 533, 640, 778
CviQI GTAC 1 cut(s) 689
DdeI CTNAG 3 cut(s) 99, 307, 415
DpnI GATC 2 cut(s) 24, 627
DpnII GATC 2 cut(s) 22, 625
EaeI YGGCCR 1 cut(s) 392
Eco47I GGWCC 1 cut(s) 185
Eco57I CTGAAG 1 cut(s) 225
EcoRII CCWGG 2 cut(s) 193, 559
FaeI CATG 3 cut(s) 510, 556, 734
FalI AAGNNNNNCTT 4 cut(s) 138, 170, 369, 401
FatI CATG 3 cut(s) 506, 552, 730
FauI CCCGC 1 cut(s) 127
Fnu4HI GCNGC 1 cut(s) 282
FokI GGATG 4 cut(s) 29, 210, 629, 710
Fsp4HI GCNGC 1 cut(s) 282
FspBI CTAG 2 cut(s) 143, 767
GluI GCNGC 1 cut(s) 282
HaeIII GGCC 1 cut(s) 394
HgaI GACGC 1 cut(s) 261
Hin1II CATG 3 cut(s) 510, 556, 734
HindIII AAGCTT 1 cut(s) 531
HinfI GANTC 1 cut(s) 35
HphI GGTGA 2 cut(s) 94, 446
Hpy188I TCNGA 4 cut(s) 116, 166, 310, 675
Hpy188III TCNNGA 4 cut(s) 174, 296, 476, 767
HpyAV CCTTC 1 cut(s) 200
HpyCH4III ACNGT 1 cut(s) 224
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 5 cut(s) 158, 338, 506, 632, 656
HpyF10VI GCNNNNNNNGC 5 cut(s) 100, 269, 278, 344, 553
HpyF3I CTNAG 3 cut(s) 99, 307, 415
HpySE526I ACGT 1 cut(s) 51
Hsp92II CATG 3 cut(s) 510, 556, 734
Kzo9I GATC 2 cut(s) 22, 625
LmnI GCTCC 2 cut(s) 289, 561
Lsp1109I GCAGC 1 cut(s) 268
LweI GCATC 4 cut(s) 81, 256, 643, 688
MaeI CTAG 2 cut(s) 143, 767
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 1 cut(s) 744
MalI GATC 2 cut(s) 24, 627
MboI GATC 2 cut(s) 22, 625
MboII GAAGA 1 cut(s) 662
MflI RGATCY 1 cut(s) 22
MhlI GDGCHC 1 cut(s) 542
MlsI TGGCCA 1 cut(s) 394
MluCI AATT 5 cut(s) 118, 159, 405, 644, 679
MluNI TGGCCA 1 cut(s) 394
MlyI GAGTC 1 cut(s) 44
MmeI TCCRAC 1 cut(s) 467
MnlI CCTC 5 cut(s) 7, 162, 201, 520, 655
Mox20I TGGCCA 1 cut(s) 394
MroXI GAANNNNTTC 1 cut(s) 669
MscI TGGCCA 1 cut(s) 394
MseI TTAA 4 cut(s) 29, 45, 582, 693
Msp20I TGGCCA 1 cut(s) 394
MspCI CTTAAG 1 cut(s) 692
MspR9I CCNGG 2 cut(s) 195, 561
MvaI CCWGG 2 cut(s) 195, 561
MwoI GCNNNNNNNGC 5 cut(s) 100, 269, 278, 344, 553
NdeII GATC 2 cut(s) 22, 625
NlaIII CATG 3 cut(s) 510, 556, 734
NmuCI GTSAC 1 cut(s) 744
NspI RCATGY 1 cut(s) 510
PaeI GCATGC 1 cut(s) 510
PdmI GAANNNNTTC 1 cut(s) 669
PflMI CCANNNNNTGG 1 cut(s) 194
PfoI TCCNGGA 1 cut(s) 193
PkrI GCNGC 1 cut(s) 283
PleI GAGTC 1 cut(s) 43
PpsI GAGTC 1 cut(s) 43
Psp1406I AACGTT 1 cut(s) 51
Psp6I CCWGG 2 cut(s) 193, 559
PspGI CCWGG 2 cut(s) 193, 559
PspPI GGNCC 1 cut(s) 185
PsuI RGATCY 1 cut(s) 22
RsaI GTAC 1 cut(s) 690
RsaNI GTAC 1 cut(s) 689
SaqAI TTAA 4 cut(s) 29, 45, 582, 693
SatI GCNGC 1 cut(s) 282
Sau3AI GATC 2 cut(s) 22, 625
Sau96I GGNCC 1 cut(s) 185
SchI GAGTC 1 cut(s) 44
ScrFI CCNGG 2 cut(s) 195, 561
SduI GDGCHC 1 cut(s) 542
SfaNI GCATC 4 cut(s) 81, 256, 643, 688
SinI GGWCC 1 cut(s) 185
SmlI CTYRAG 1 cut(s) 692
SmoI CTYRAG 1 cut(s) 692
SphI GCATGC 1 cut(s) 510
Sse9I AATT 5 cut(s) 118, 159, 405, 644, 679
SsiI CCGC 2 cut(s) 39, 134
SspI AATATT 1 cut(s) 572
SspMI CTAG 2 cut(s) 143, 767
StyD4I CCNGG 2 cut(s) 193, 559
TaaI ACNGT 1 cut(s) 224
TaiI ACGT 1 cut(s) 54
TasI AATT 5 cut(s) 118, 159, 405, 644, 679
TatI WGTACW 1 cut(s) 688
Tru1I TTAA 4 cut(s) 29, 45, 582, 693
Tru9I TTAA 4 cut(s) 29, 45, 582, 693
TscAI CASTG 1 cut(s) 229
TseFI GTSAC 1 cut(s) 744
TseI GCWGC 1 cut(s) 281
Tsp45I GTSAC 1 cut(s) 744
TspDTI ATGAA 1 cut(s) 609
TspGWI ACGGA 2 cut(s) 276, 412
TspRI CASTG 1 cut(s) 229
Van91I CCANNNNNTGG 1 cut(s) 194
Vha464I CTTAAG 1 cut(s) 692
VpaK11BI GGWCC 1 cut(s) 185
XapI RAATTY 1 cut(s) 679
XbaI TCTAGA 1 cut(s) 766
XceI RCATGY 1 cut(s) 510
XmnI GAANNNNTTC 1 cut(s) 669
XspI CTAG 2 cut(s) 143, 767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.