pycom05g31600

L-ascorbate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0029290
Physical Location & Seq
Forward (+)
31297217 .. 31297840
624 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 393 bp
ATGCACGCTAACTTCCGAGAATACGTCGAGATTGTGTTCCAAAATTGGGAAGACACAGTGCAGTCATGGCACATTGATGGCTATTCATTCTTTGTCATGGGGATGGAAGGAGGACAATGGACACCTGCAAGTAGAGCTCGTTACAATTTCAGAGACACAGTTGCACGTTCCACAACTCAGGTGTATCCTAGGGCATGGACGGCAATCTACATAGCATTGGACAGTGTGGGAATGTGGAACGTCAGGTCTCAAAATTGGGGGAGGCAGTACTTGGGGCAGCAGTTCTATCTGAGAGTGTATTCTCCTGCACATTCATGGAGAGATGAATACCCAATTCCAAGAAATGCCCTTCTTTGTGGAAGGGCGAGAGGCCGTCACACTAGGCCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

131

Amino Acids

15.47

Weight (kDa)

9.72

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_2 PF07731 2 - 102 4.8e-28 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0029290)

Species Orthologous Gene IDs
pyrus_communis pycom05g31600
rosa_multiflora Rmu_co8443833.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 133
Acc36I ACCTGC 1 cut(s) 133
AfaI GTAC 1 cut(s) 269
AfiI CCNNNNNNNGG 1 cut(s) 46
AluBI AGCT 1 cut(s) 137
AluI AGCT 1 cut(s) 137
Alw21I GWGCWC 1 cut(s) 139
Alw26I GTCTC 2 cut(s) 147, 252
AoxI GGCC 2 cut(s) 370, 383
ApeKI GCWGC 1 cut(s) 277
AspA2I CCTAGG 1 cut(s) 188
AvrII CCTAGG 1 cut(s) 188
BanII GRGCYC 1 cut(s) 139
BbsI GAAGAC 1 cut(s) 57
Bbv12I GWGCWC 1 cut(s) 139
BbvI GCAGC 1 cut(s) 289
BccI CCATC 2 cut(s) 71, 97
BceAI ACGGC 2 cut(s) 216, 357
BciVI GTATCC 1 cut(s) 195
BcoDI GTCTC 2 cut(s) 147, 252
BfaI CTAG 2 cut(s) 189, 381
BfuAI ACCTGC 1 cut(s) 133
BfuI GTATCC 1 cut(s) 195
BisI GCNGC 1 cut(s) 278
BlnI CCTAGG 1 cut(s) 188
BlsI GCNGC 1 cut(s) 279
BmcAI AGTACT 1 cut(s) 269
BpiI GAAGAC 1 cut(s) 57
BsaI GGTCTC 1 cut(s) 252
BsaJI CCNNGG 1 cut(s) 188
Bsc4I CCNNNNNNNGG 1 cut(s) 46
BseDI CCNNGG 1 cut(s) 188
BseGI GGATG 1 cut(s) 108
BseLI CCNNNNNNNGG 1 cut(s) 46
BseMII CTCAG 2 cut(s) 191, 281
BseXI GCAGC 1 cut(s) 289
BsgI GTGCAG 2 cut(s) 80, 291
BshFI GGCC 2 cut(s) 372, 385
BsiHKAI GWGCWC 1 cut(s) 139
BslI CCNNNNNNNGG 1 cut(s) 46
BsmAI GTCTC 2 cut(s) 147, 252
BsnI GGCC 2 cut(s) 372, 385
Bso31I GGTCTC 1 cut(s) 252
Bsp1286I GDGCHC 1 cut(s) 139
BspANI GGCC 2 cut(s) 372, 385
BspCNI CTCAG 2 cut(s) 190, 282
BspMI ACCTGC 1 cut(s) 133
BspTNI GGTCTC 1 cut(s) 252
BssECI CCNNGG 1 cut(s) 188
BssT1I CCWWGG 1 cut(s) 188
Bst4CI ACNGT 3 cut(s) 58, 160, 224
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 2 cut(s) 177, 290
BstF5I GGATG 1 cut(s) 108
BstMAI GTCTC 2 cut(s) 147, 252
BstMWI GCNNNNNNNGC 3 cut(s) 67, 134, 200
BstV1I GCAGC 1 cut(s) 289
BstV2I GAAGAC 1 cut(s) 57
BsuI GTATCC 1 cut(s) 195
BsuRI GGCC 2 cut(s) 372, 385
BtsCI GGATG 1 cut(s) 108
BtsIMutI CAGTG 2 cut(s) 63, 229
BveI ACCTGC 1 cut(s) 133
Cac8I GCNNGC 1 cut(s) 6
Csp6I GTAC 1 cut(s) 268
CviAII CATG 4 cut(s) 66, 97, 195, 315
CviJI RGCY 4 cut(s) 81, 137, 372, 385
CviKI_1 RGCY 4 cut(s) 81, 137, 372, 385
CviQI GTAC 1 cut(s) 268
DdeI CTNAG 2 cut(s) 177, 290
Ecl136II GAGCTC 1 cut(s) 137
Eco130I CCWWGG 1 cut(s) 188
Eco147I AGGCCT 1 cut(s) 385
Eco24I GRGCYC 1 cut(s) 139
Eco31I GGTCTC 1 cut(s) 252
Eco53kI GAGCTC 1 cut(s) 137
EcoICRI GAGCTC 1 cut(s) 137
EcoT14I CCWWGG 1 cut(s) 188
EcoT38I GRGCYC 1 cut(s) 139
ErhI CCWWGG 1 cut(s) 188
FaeI CATG 4 cut(s) 69, 100, 198, 318
FaiI YATR 5 cut(s) 67, 98, 196, 212, 316
FatI CATG 4 cut(s) 65, 96, 194, 314
Fnu4HI GCNGC 1 cut(s) 278
FokI GGATG 1 cut(s) 115
FriOI GRGCYC 1 cut(s) 139
Fsp4HI GCNGC 1 cut(s) 278
FspBI CTAG 2 cut(s) 189, 381
GluI GCNGC 1 cut(s) 278
HaeIII GGCC 2 cut(s) 372, 385
Hin1II CATG 4 cut(s) 69, 100, 198, 318
Hpy188I TCNGA 3 cut(s) 17, 152, 291
Hpy188III TCNNGA 1 cut(s) 28
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 3 cut(s) 101, 354, 359
HpyCH4III ACNGT 3 cut(s) 58, 160, 224
HpyCH4IV ACGT 3 cut(s) 24, 166, 240
HpyCH4V TGCA 5 cut(s) 4, 61, 128, 164, 308
HpyF10VI GCNNNNNNNGC 3 cut(s) 67, 134, 200
HpyF3I CTNAG 2 cut(s) 177, 290
HpySE526I ACGT 3 cut(s) 24, 166, 240
Hsp92II CATG 4 cut(s) 69, 100, 198, 318
LpnPI CCDG 4 cut(s) 138, 164, 229, 318
Lsp1109I GCAGC 1 cut(s) 289
MaeI CTAG 2 cut(s) 189, 381
MaeII ACGT 3 cut(s) 24, 166, 240
MaeIII GTNAC 2 cut(s) 140, 374
MboII GAAGA 1 cut(s) 62
MhlI GDGCHC 1 cut(s) 139
MluCI AATT 4 cut(s) 43, 145, 253, 333
MnlI CCTC 3 cut(s) 104, 255, 362
MslI CAYNNNNRTG 3 cut(s) 75, 101, 313
MwoI GCNNNNNNNGC 3 cut(s) 67, 134, 200
NlaIII CATG 4 cut(s) 69, 100, 198, 318
NmuCI GTSAC 1 cut(s) 374
PaqCI CACCTGC 1 cut(s) 133
PceI AGGCCT 1 cut(s) 385
PkrI GCNGC 1 cut(s) 279
Psp124BI GAGCTC 1 cut(s) 139
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
RseI CAYNNNNRTG 3 cut(s) 75, 101, 313
SacI GAGCTC 1 cut(s) 139
SatI GCNGC 1 cut(s) 278
ScaI AGTACT 1 cut(s) 269
SduI GDGCHC 1 cut(s) 139
SetI ASST 7 cut(s) 27, 127, 139, 169, 183, 243, 248
SmiMI CAYNNNNRTG 3 cut(s) 75, 101, 313
Sse9I AATT 4 cut(s) 43, 145, 253, 333
SseBI AGGCCT 1 cut(s) 385
SspMI CTAG 2 cut(s) 189, 381
SstI GAGCTC 1 cut(s) 139
StuI AGGCCT 1 cut(s) 385
StyI CCWWGG 1 cut(s) 188
TaaI ACNGT 3 cut(s) 58, 160, 224
TaiI ACGT 3 cut(s) 27, 169, 243
TaqI TCGA 1 cut(s) 27
TasI AATT 4 cut(s) 43, 145, 253, 333
TatI WGTACW 1 cut(s) 267
TscAI CASTG 2 cut(s) 63, 229
TseFI GTSAC 1 cut(s) 374
TseI GCWGC 1 cut(s) 277
Tsp45I GTSAC 1 cut(s) 374
TspDTI ATGAA 3 cut(s) 75, 303, 339
TspRI CASTG 2 cut(s) 63, 229
XmaJI CCTAGG 1 cut(s) 188
XspI CTAG 2 cut(s) 189, 381
ZrmI AGTACT 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.