pycom06g00880

Plastidial lipoyltransferase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0029293
Physical Location & Seq
Forward (+)
829547 .. 830572
1026 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 576 bp
ATGATCATTGCCGAGACTCATTGTTCGTTCTTCAGCATCGTCCTGTGTATACCTTGGGAACTGCTAGTTCAGAACAGTTCCTTAATTTTGATATCAAGGACCCCCCTTTCGATGTTTATCGAACTGAACGTGGCGGCGAGGTTACATATCATGGTCCGGGTCAGGTCACTTGAGGAGGTGATCATCCGTGCTCTATCAAAGACATTCTGTATCAAGGCATCTCGGCTTGAGGGCCTCACTGGTGTTTGGCATGGAAACCAGAAACTGGCAGCCATTGGAATACGAGTCAGCCAGTGGATAACATATCATGGCTTAGCAGTGAATGTGAACCCAGACTTGACCCATTTTCGTTGGATCGTACCTTGTGGGTTACAAGGCTATCAGGTTGGAAGCGTTAAAAGCTTGCTTGAGGAATTTCATACATCTGCCGACTGTGGAAGAGCAGATCTACCTGATCCCGATGATTGTCAGCTACTTGATATTACTTGTAGATCTTTGATCGATGAGTTTTCTGAAGTTTTTGAGGTTAGAATCAATTATGAAACCATGTCAAGGTTGGATTTACAAGCCGTCTGA

Protein Analysis

192

Amino Acids

21.57

Weight (kDa)

5.65

Isoelectric Point (pI)

32.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 47 - 139 8.8e-17 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029293)

Species Orthologous Gene IDs
pyrus_communis pycom06g00880
rosa_samantha Rh5DG520200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 265
AccI GTMKAC 1 cut(s) 49
AciI CCGC 1 cut(s) 134
AclWI GGATC 2 cut(s) 362, 449
AcsI RAATTY 1 cut(s) 413
AcuI CTGAAG 2 cut(s) 16, 534
AfaI GTAC 1 cut(s) 360
AfiI CCNNNNNNNGG 2 cut(s) 265, 552
AluBI AGCT 2 cut(s) 402, 472
AluI AGCT 2 cut(s) 402, 472
Alw21I GWGCWC 1 cut(s) 193
Alw26I GTCTC 1 cut(s) 8
AlwI GGATC 2 cut(s) 362, 449
AlwNI CAGNNNCTG 1 cut(s) 265
AoxI GGCC 1 cut(s) 232
ApeKI GCWGC 1 cut(s) 269
ApoI RAATTY 1 cut(s) 413
ArsI GACNNNNNNTTYG 4 cut(s) 7, 39, 91, 123
AspS9I GGNCC 3 cut(s) 99, 154, 232
AsuC2I CCSGG 1 cut(s) 158
AsuHPI GGTGA 1 cut(s) 190
AvaII GGWCC 2 cut(s) 99, 154
Bbv12I GWGCWC 1 cut(s) 193
BbvI GCAGC 1 cut(s) 281
BceAI ACGGC 1 cut(s) 554
BclI TGATCA 2 cut(s) 3, 180
BcnI CCSGG 1 cut(s) 158
BcoDI GTCTC 1 cut(s) 8
BfaI CTAG 1 cut(s) 65
BglII AGATCT 2 cut(s) 445, 491
BisI GCNGC 2 cut(s) 135, 270
BlpI GCTNAGC 1 cut(s) 313
BlsI GCNGC 2 cut(s) 136, 271
Bme1390I CCNGG 1 cut(s) 158
Bme18I GGWCC 2 cut(s) 99, 154
BmgT120I GGNCC 3 cut(s) 99, 154, 232
BmiI GGNNCC 1 cut(s) 101
BmrFI CCNGG 1 cut(s) 158
BmsI GCATC 2 cut(s) 45, 227
Bpu1102I GCTNAGC 1 cut(s) 313
BpuEI CTTGAG 3 cut(s) 191, 248, 428
BpuMI CCSGG 1 cut(s) 158
Bsa29I ATCGAT 1 cut(s) 501
BsaBI GATNNNNATC 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 53
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 552
Bse1I ACTGG 3 cut(s) 244, 270, 292
Bse3DI GCAATG 1 cut(s) 6
Bse8I GATNNNNATC 1 cut(s) 116
BseCI ATCGAT 1 cut(s) 501
BseDI CCNNGG 1 cut(s) 53
BseGI GGATG 1 cut(s) 183
BseJI GATNNNNATC 1 cut(s) 116
BseLI CCNNNNNNNGG 2 cut(s) 265, 552
BseMI GCAATG 1 cut(s) 6
BseNI ACTGG 3 cut(s) 244, 270, 292
BseRI GAGGAG 1 cut(s) 188
BseXI GCAGC 1 cut(s) 281
BshFI GGCC 1 cut(s) 234
BshVI ATCGAT 1 cut(s) 501
BsiHKAI GWGCWC 1 cut(s) 193
BsiSI CCGG 1 cut(s) 157
BslI CCNNNNNNNGG 2 cut(s) 265, 552
BsmAI GTCTC 1 cut(s) 8
BsnI GGCC 1 cut(s) 234
Bsp1286I GDGCHC 1 cut(s) 193
Bsp143I GATC 7 cut(s) 3, 180, 354, 445, 454, 491, 498
Bsp1720I GCTNAGC 1 cut(s) 313
BspACI CCGC 1 cut(s) 134
BspANI GGCC 1 cut(s) 234
BspDI ATCGAT 1 cut(s) 501
BspLI GGNNCC 1 cut(s) 101
BspPI GGATC 2 cut(s) 362, 449
BspQI GCTCTTC 1 cut(s) 433
BsrDI GCAATG 1 cut(s) 6
BsrI ACTGG 3 cut(s) 244, 270, 292
BssECI CCNNGG 1 cut(s) 53
BssMI GATC 7 cut(s) 3, 180, 354, 445, 454, 491, 498
BssNAI GTATAC 1 cut(s) 50
BssT1I CCWWGG 1 cut(s) 53
Bst1107I GTATAC 1 cut(s) 50
Bst4CI ACNGT 2 cut(s) 77, 434
Bst6I CTCTTC 1 cut(s) 433
BstC8I GCNNGC 1 cut(s) 404
BstDEI CTNAG 1 cut(s) 313
BstF5I GGATG 1 cut(s) 183
BstKTI GATC 7 cut(s) 6, 183, 357, 448, 457, 494, 501
BstMAI GTCTC 1 cut(s) 8
BstMBI GATC 7 cut(s) 3, 180, 354, 445, 454, 491, 498
BstMWI GCNNNNNNNGC 1 cut(s) 399
BstSCI CCNGG 1 cut(s) 156
BstV1I GCAGC 1 cut(s) 281
BstX2I RGATCY 2 cut(s) 445, 491
BstYI RGATCY 2 cut(s) 445, 491
BstZ17I GTATAC 1 cut(s) 50
Bsu15I ATCGAT 1 cut(s) 501
BsuRI GGCC 1 cut(s) 234
BsuTUI ATCGAT 1 cut(s) 501
BtsCI GGATG 1 cut(s) 183
BtsI GCAGTG 1 cut(s) 324
BtsIMutI CAGTG 3 cut(s) 237, 299, 324
Cac8I GCNNGC 1 cut(s) 404
CaiI CAGNNNCTG 1 cut(s) 265
Cfr13I GGNCC 3 cut(s) 99, 154, 232
ClaI ATCGAT 1 cut(s) 501
Csp6I GTAC 1 cut(s) 359
CviAII CATG 4 cut(s) 151, 251, 308, 547
CviJI RGCY 9 cut(s) 226, 234, 272, 291, 312, 378, 402, 472, 569
CviKI_1 RGCY 9 cut(s) 226, 234, 272, 291, 312, 378, 402, 472, 569
CviQI GTAC 1 cut(s) 359
DdeI CTNAG 1 cut(s) 313
DpnI GATC 7 cut(s) 5, 182, 356, 447, 456, 493, 500
DpnII GATC 7 cut(s) 3, 180, 354, 445, 454, 491, 498
Eam1104I CTCTTC 1 cut(s) 433
EarI CTCTTC 1 cut(s) 433
Eco130I CCWWGG 1 cut(s) 53
Eco32I GATATC 1 cut(s) 93
Eco47I GGWCC 2 cut(s) 99, 154
Eco57I CTGAAG 2 cut(s) 16, 534
EcoO109I RGGNCCY 2 cut(s) 99, 232
EcoRV GATATC 1 cut(s) 93
EcoT14I CCWWGG 1 cut(s) 53
ErhI CCWWGG 1 cut(s) 53
FaeI CATG 4 cut(s) 154, 254, 311, 550
FaiI YATR 9 cut(s) 50, 147, 152, 252, 304, 309, 420, 540, 548
FatI CATG 4 cut(s) 150, 250, 307, 546
FbaI TGATCA 2 cut(s) 3, 180
FblI GTMKAC 1 cut(s) 49
Fnu4HI GCNGC 2 cut(s) 135, 270
FokI GGATG 1 cut(s) 170
Fsp4HI GCNGC 2 cut(s) 135, 270
FspBI CTAG 1 cut(s) 65
GluI GCNGC 2 cut(s) 135, 270
HaeIII GGCC 1 cut(s) 234
HapII CCGG 1 cut(s) 157
Hin1II CATG 4 cut(s) 154, 254, 311, 550
HindIII AAGCTT 1 cut(s) 400
HinfI GANTC 3 cut(s) 16, 285, 531
HpaII CCGG 1 cut(s) 157
HphI GGTGA 1 cut(s) 190
Hpy166II GTNNAC 2 cut(s) 50, 328
Hpy188I TCNGA 3 cut(s) 72, 514, 575
Hpy188III TCNNGA 1 cut(s) 458
Hpy8I GTNNAC 2 cut(s) 50, 328
HpyCH4III ACNGT 2 cut(s) 77, 434
HpyCH4IV ACGT 1 cut(s) 129
HpyF10VI GCNNNNNNNGC 1 cut(s) 399
HpyF3I CTNAG 1 cut(s) 313
HpySE526I ACGT 1 cut(s) 129
Hsp92II CATG 4 cut(s) 154, 254, 311, 550
Ksp22I TGATCA 2 cut(s) 3, 180
Kzo9I GATC 7 cut(s) 3, 180, 354, 445, 454, 491, 498
LguI GCTCTTC 1 cut(s) 433
Lsp1109I GCAGC 1 cut(s) 281
LweI GCATC 2 cut(s) 45, 227
MaeI CTAG 1 cut(s) 65
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 3 cut(s) 141, 165, 369
MalI GATC 7 cut(s) 5, 182, 356, 447, 456, 493, 500
MboI GATC 7 cut(s) 3, 180, 354, 445, 454, 491, 498
MboII GAAGA 2 cut(s) 22, 450
MflI RGATCY 2 cut(s) 445, 491
MhlI GDGCHC 1 cut(s) 193
MluCI AATT 3 cut(s) 84, 413, 535
MlyI GAGTC 2 cut(s) 10, 294
MmeI TCCRAC 3 cut(s) 332, 367, 537
MnlI CCTC 7 cut(s) 132, 166, 169, 223, 245, 403, 517
MseI TTAA 2 cut(s) 83, 396
MspI CCGG 1 cut(s) 157
MspR9I CCNGG 1 cut(s) 158
MwoI GCNNNNNNNGC 1 cut(s) 399
NciI CCSGG 1 cut(s) 158
NdeII GATC 7 cut(s) 3, 180, 354, 445, 454, 491, 498
NlaIII CATG 4 cut(s) 154, 254, 311, 550
NlaIV GGNNCC 1 cut(s) 101
NmeAIII GCCGAG 2 cut(s) 37, 202
NmuCI GTSAC 1 cut(s) 165
PciSI GCTCTTC 1 cut(s) 433
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PfeI GAWTC 1 cut(s) 531
PflMI CCANNNNNTGG 1 cut(s) 265
PkrI GCNGC 2 cut(s) 136, 271
PleI GAGTC 2 cut(s) 10, 293
PpsI GAGTC 2 cut(s) 10, 293
PpuMI RGGWCCY 1 cut(s) 99
Psp5II RGGWCCY 1 cut(s) 99
PspN4I GGNNCC 1 cut(s) 101
PspPI GGNCC 3 cut(s) 99, 154, 232
PspPPI RGGWCCY 1 cut(s) 99
PstNI CAGNNNCTG 1 cut(s) 265
PsuI RGATCY 2 cut(s) 445, 491
RsaI GTAC 1 cut(s) 360
RsaNI GTAC 1 cut(s) 359
SapI GCTCTTC 1 cut(s) 433
SaqAI TTAA 2 cut(s) 83, 396
SatI GCNGC 2 cut(s) 135, 270
Sau3AI GATC 7 cut(s) 3, 180, 354, 445, 454, 491, 498
Sau96I GGNCC 3 cut(s) 99, 154, 232
SchI GAGTC 2 cut(s) 10, 294
ScrFI CCNGG 1 cut(s) 158
SduI GDGCHC 1 cut(s) 193
SfaNI GCATC 2 cut(s) 45, 227
SinI GGWCC 2 cut(s) 99, 154
SmlI CTYRAG 3 cut(s) 170, 227, 407
SmoI CTYRAG 3 cut(s) 170, 227, 407
Sse9I AATT 3 cut(s) 84, 413, 535
SsiI CCGC 1 cut(s) 134
SspMI CTAG 1 cut(s) 65
StyD4I CCNGG 1 cut(s) 156
StyI CCWWGG 1 cut(s) 53
TaaI ACNGT 2 cut(s) 77, 434
TaiI ACGT 1 cut(s) 132
TaqI TCGA 3 cut(s) 110, 120, 501
TasI AATT 3 cut(s) 84, 413, 535
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 1 cut(s) 531
Tru1I TTAA 2 cut(s) 83, 396
Tru9I TTAA 2 cut(s) 83, 396
TscAI CASTG 3 cut(s) 244, 299, 324
TseFI GTSAC 1 cut(s) 165
TseI GCWGC 1 cut(s) 269
Tsp45I GTSAC 1 cut(s) 165
TspDTI ATGAA 2 cut(s) 407, 555
TspGWI ACGGA 1 cut(s) 176
TspRI CASTG 3 cut(s) 244, 299, 324
Van91I CCANNNNNTGG 1 cut(s) 265
VpaK11BI GGWCC 2 cut(s) 99, 154
XapI RAATTY 1 cut(s) 413
XcmI CCANNNNNNNNNTGG 1 cut(s) 553
XmiI GTMKAC 1 cut(s) 49
XspI CTAG 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.