Rh5DG520200

Plastidial lipoyltransferase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0029293
Physical Location & Seq
Reverse (-)
80819814 .. 80820266
453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG520200.1

Sequence Viewer

Length: 357 bp
ATGGATCATCATTGGTACCTCAGGTCACTTGAGGAGGTGATCATTCGCGCTCTTTCAAAGATATTTTCGATCAAGGCATCTCGACTTGAGGGCTTAACTGGTGTTTGGCATGGAAACCAAAAACTGGCAGCCATTGGTATACGAGTGTCTCAGTGGATAGCTTATCACGGTTTGGCACTGAACGTGACCACAGATTTAACTCCTTTTCGTTGGATAGTACCGTGTGGTTTACAAAACTATCAGGTTGGAAGCATTAGAGGGTTGTTGGGGGAGTTTGAGTCATTTGACAATAGTAGTAGAAAACAGCTACCAGATCCTGATGATGGCCAGCTCCTTGATATTGCTTGTAAATCTTGA

Protein Analysis

118

Amino Acids

13.29

Weight (kDa)

6.9

Isoelectric Point (pI)

35.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 2 - 91 7.5e-18 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0029293)

Species Orthologous Gene IDs
pyrus_communis pycom06g00880
rosa_samantha Rh5DG520200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 15
AccB1I GGYRCC 1 cut(s) 15
AccB7I CCANNNNNTGG 1 cut(s) 124
AccI GTMKAC 1 cut(s) 139
AccII CGCG 1 cut(s) 48
AclWI GGATC 2 cut(s) 12, 308
AcoI YGGCCR 1 cut(s) 325
AfaI GTAC 2 cut(s) 17, 219
AfiI CCNNNNNNNGG 2 cut(s) 124, 323
AgsI TTSAA 1 cut(s) 57
AluBI AGCT 3 cut(s) 161, 307, 331
AluI AGCT 3 cut(s) 161, 307, 331
Alw26I GTCTC 1 cut(s) 153
AlwI GGATC 2 cut(s) 12, 308
AlwNI CAGNNNCTG 1 cut(s) 317
AoxI GGCC 1 cut(s) 325
ApeKI GCWGC 1 cut(s) 128
Asp718I GGTACC 1 cut(s) 15
AspLEI GCGC 1 cut(s) 50
AsuHPI GGTGA 1 cut(s) 49
AxyI CCTNAGG 1 cut(s) 20
BalI TGGCCA 1 cut(s) 327
BanI GGYRCC 1 cut(s) 15
BbvI GCAGC 1 cut(s) 140
BccI CCATC 1 cut(s) 317
BclI TGATCA 1 cut(s) 39
BcoDI GTCTC 1 cut(s) 153
BisI GCNGC 1 cut(s) 129
BlsI GCNGC 1 cut(s) 130
BmiI GGNNCC 1 cut(s) 17
BmsI GCATC 1 cut(s) 86
BpuEI CTTGAG 2 cut(s) 50, 107
BsaXI ACNNNNNCTCC 2 cut(s) 263, 293
Bsc4I CCNNNNNNNGG 2 cut(s) 124, 323
Bse1I ACTGG 2 cut(s) 103, 129
Bse21I CCTNAGG 1 cut(s) 20
BseLI CCNNNNNNNGG 2 cut(s) 124, 323
BseMII CTCAG 2 cut(s) 34, 164
BseNI ACTGG 2 cut(s) 103, 129
BseRI GAGGAG 1 cut(s) 47
BseXI GCAGC 1 cut(s) 140
Bsh1236I CGCG 1 cut(s) 48
BshFI GGCC 1 cut(s) 327
BshNI GGYRCC 1 cut(s) 15
BslI CCNNNNNNNGG 2 cut(s) 124, 323
BsmAI GTCTC 1 cut(s) 153
BsnI GGCC 1 cut(s) 327
Bsp143I GATC 4 cut(s) 4, 39, 69, 313
BspANI GGCC 1 cut(s) 327
BspCNI CTCAG 2 cut(s) 33, 163
BspFNI CGCG 1 cut(s) 48
BspLI GGNNCC 1 cut(s) 17
BspPI GGATC 2 cut(s) 12, 308
BspT107I GGYRCC 1 cut(s) 15
BsrI ACTGG 2 cut(s) 103, 129
BssMI GATC 4 cut(s) 4, 39, 69, 313
BssNAI GTATAC 1 cut(s) 140
Bst1107I GTATAC 1 cut(s) 140
Bst4CI ACNGT 2 cut(s) 170, 222
BstC8I GCNNGC 1 cut(s) 329
BstDEI CTNAG 2 cut(s) 20, 150
BstFNI CGCG 1 cut(s) 48
BstHHI GCGC 1 cut(s) 50
BstKTI GATC 4 cut(s) 7, 42, 72, 316
BstMAI GTCTC 1 cut(s) 153
BstMBI GATC 4 cut(s) 4, 39, 69, 313
BstUI CGCG 1 cut(s) 48
BstV1I GCAGC 1 cut(s) 140
BstX2I RGATCY 1 cut(s) 313
BstYI RGATCY 1 cut(s) 313
BstZ17I GTATAC 1 cut(s) 140
Bsu36I CCTNAGG 1 cut(s) 20
BsuRI GGCC 1 cut(s) 327
BtsIMutI CAGTG 2 cut(s) 158, 176
Cac8I GCNNGC 1 cut(s) 329
CaiI CAGNNNCTG 1 cut(s) 317
CfoI GCGC 1 cut(s) 50
Csp6I GTAC 2 cut(s) 16, 218
CviAII CATG 1 cut(s) 110
CviJI RGCY 6 cut(s) 93, 131, 161, 307, 327, 331
CviKI_1 RGCY 6 cut(s) 93, 131, 161, 307, 327, 331
CviQI GTAC 2 cut(s) 16, 218
DdeI CTNAG 2 cut(s) 20, 150
DpnI GATC 4 cut(s) 6, 41, 71, 315
DpnII GATC 4 cut(s) 4, 39, 69, 313
EaeI YGGCCR 1 cut(s) 325
Eco81I CCTNAGG 1 cut(s) 20
FaeI CATG 1 cut(s) 113
FaiI YATR 2 cut(s) 111, 140
FatI CATG 1 cut(s) 109
FbaI TGATCA 1 cut(s) 39
FblI GTMKAC 1 cut(s) 139
Fnu4HI GCNGC 1 cut(s) 129
Fsp4HI GCNGC 1 cut(s) 129
GlaI GCGC 1 cut(s) 49
GluI GCNGC 1 cut(s) 129
HaeIII GGCC 1 cut(s) 327
HhaI GCGC 1 cut(s) 50
Hin1II CATG 1 cut(s) 113
Hin6I GCGC 1 cut(s) 48
HinP1I GCGC 1 cut(s) 48
HinfI GANTC 1 cut(s) 278
HphI GGTGA 1 cut(s) 49
Hpy166II GTNNAC 2 cut(s) 140, 230
Hpy188III TCNNGA 3 cut(s) 81, 317, 354
Hpy8I GTNNAC 2 cut(s) 140, 230
HpyCH4III ACNGT 2 cut(s) 170, 222
HpyCH4IV ACGT 1 cut(s) 183
HpyF3I CTNAG 2 cut(s) 20, 150
HpySE526I ACGT 1 cut(s) 183
Hsp92II CATG 1 cut(s) 113
HspAI GCGC 1 cut(s) 48
KpnI GGTACC 1 cut(s) 19
Ksp22I TGATCA 1 cut(s) 39
Kzo9I GATC 4 cut(s) 4, 39, 69, 313
LmnI GCTCC 1 cut(s) 336
LpnPI CCDG 7 cut(s) 7, 84, 110, 227, 324, 330, 341
Lsp1109I GCAGC 1 cut(s) 140
LweI GCATC 1 cut(s) 86
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 2 cut(s) 24, 184
MalI GATC 4 cut(s) 6, 41, 71, 315
MboI GATC 4 cut(s) 4, 39, 69, 313
MflI RGATCY 1 cut(s) 313
MlsI TGGCCA 1 cut(s) 327
MluNI TGGCCA 1 cut(s) 327
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 2 cut(s) 191, 226
MnlI CCTC 5 cut(s) 25, 28, 29, 82, 251
Mox20I TGGCCA 1 cut(s) 327
MscI TGGCCA 1 cut(s) 327
MseI TTAA 2 cut(s) 95, 197
Msp20I TGGCCA 1 cut(s) 327
MvnI CGCG 1 cut(s) 48
NdeII GATC 4 cut(s) 4, 39, 69, 313
NlaIII CATG 1 cut(s) 113
NlaIV GGNNCC 1 cut(s) 17
NmuCI GTSAC 2 cut(s) 24, 184
PflMI CCANNNNNTGG 1 cut(s) 124
PkrI GCNGC 1 cut(s) 130
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
PspN4I GGNNCC 1 cut(s) 17
PstNI CAGNNNCTG 1 cut(s) 317
PsuI RGATCY 1 cut(s) 313
RsaI GTAC 2 cut(s) 17, 219
RsaNI GTAC 2 cut(s) 16, 218
SaqAI TTAA 2 cut(s) 95, 197
SatI GCNGC 1 cut(s) 129
Sau3AI GATC 4 cut(s) 4, 39, 69, 313
SchI GAGTC 1 cut(s) 287
SetI ASST 8 cut(s) 21, 26, 39, 163, 186, 246, 309, 333
SfaNI GCATC 1 cut(s) 86
SmlI CTYRAG 2 cut(s) 29, 86
SmoI CTYRAG 2 cut(s) 29, 86
TaaI ACNGT 2 cut(s) 170, 222
TaiI ACGT 1 cut(s) 186
TaqI TCGA 2 cut(s) 68, 82
Tru1I TTAA 2 cut(s) 95, 197
Tru9I TTAA 2 cut(s) 95, 197
TscAI CASTG 2 cut(s) 158, 183
TseFI GTSAC 2 cut(s) 24, 184
TseI GCWGC 1 cut(s) 128
Tsp45I GTSAC 2 cut(s) 24, 184
TspRI CASTG 2 cut(s) 158, 183
Van91I CCANNNNNTGG 1 cut(s) 124
XmiI GTMKAC 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.