pycom06g18390

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0029303
Physical Location & Seq
Forward (+)
22182693 .. 22184067
1375 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 588 bp
ATGTTTGTAGGCGGAAGAGAGAATATACAGAGATTGATGTGCAAGGTGGAGAAGAAGGAGAGAAAGGGAGTGATGGGATTTAAGGTGTTGGGAGGCGGCGGAGGAGGAGGGGAAAAGTTAAAGAACTCGATGCTGGTCACGGCGGCGTCGTCGCCGTCGCCGTCGAGGTCGAAAATGAAGCTGTGGATGATCCGTGCGACTTCGTCGGTTTTGTTGTGGACGTGCATCGTGCAATTAACGGCTCTGGGGGATATGTGGGGTCCTAGGGTTTTGAAAGGGTGGCCTGCTTGTTTCTCTCTGGAGTCCGCCGCCGCCTCCTCCGTCTTGCAAGACAGGTTGCCTTCTCTTCCGGCCAGAGCTCTTCCGCCCAAGAGGGTTTATAAAAACAATGGTTACCTGATGGTATCATGCAATGGGGGACTCAATCAAATGAGAGCAGCGATATGTGACATGGTTGCTATCGCAAGATACTTGAACGTCACTCTCATAGTCCCTGAACTCGACAAAACCTCCTTTTGGGCTGATCCCAGTGAATTCAAAGACATATTTGATGTGGATCGATCGATCGGTATTTCATCACATCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

196

Amino Acids

21.28

Weight (kDa)

9.71

Isoelectric Point (pI)

44.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 132 - 187 8.8e-23 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029303)

Species Orthologous Gene IDs
pyrus_communis pycom06g18390
rosa_multiflora Rmu_co8314229.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 381
AciI CCGC 8 cut(s) 12, 96, 99, 143, 306, 309, 312, 365
AclWI GGATC 3 cut(s) 184, 518, 564
AcoI YGGCCR 1 cut(s) 351
AcsI RAATTY 1 cut(s) 533
AcyI GRCGYC 1 cut(s) 146
AfiI CCNNNNNNNGG 1 cut(s) 516
AgsI TTSAA 3 cut(s) 274, 475, 538
AjiI CACGTC 1 cut(s) 222
AluBI AGCT 2 cut(s) 181, 359
AluI AGCT 2 cut(s) 181, 359
Alw21I GWGCWC 1 cut(s) 361
AlwI GGATC 3 cut(s) 184, 518, 564
AoxI GGCC 2 cut(s) 281, 351
ApeKI GCWGC 1 cut(s) 437
ApoI RAATTY 1 cut(s) 533
AspA2I CCTAGG 1 cut(s) 263
AspS9I GGNCC 1 cut(s) 260
AvaII GGWCC 1 cut(s) 260
AvrII CCTAGG 1 cut(s) 263
BanII GRGCYC 1 cut(s) 361
Bbv12I GWGCWC 1 cut(s) 361
BbvI GCAGC 1 cut(s) 449
BccI CCATC 2 cut(s) 67, 394
BceAI ACGGC 4 cut(s) 139, 145, 156, 255
BfaI CTAG 1 cut(s) 264
BisI GCNGC 5 cut(s) 97, 144, 309, 312, 438
BlnI CCTAGG 1 cut(s) 263
BlsI GCNGC 5 cut(s) 98, 145, 310, 313, 439
Bme18I GGWCC 1 cut(s) 260
BmgBI CACGTC 1 cut(s) 222
BmgT120I GGNCC 1 cut(s) 260
BmiI GGNNCC 1 cut(s) 261
BmrI ACTGGG 1 cut(s) 522
BmsI GCATC 2 cut(s) 120, 234
BmuI ACTGGG 1 cut(s) 522
BpmI CTGGAG 1 cut(s) 320
Bsa29I ATCGAT 2 cut(s) 559, 563
BsaBI GATNNNNATC 1 cut(s) 555
BsaHI GRCGYC 1 cut(s) 146
BsaJI CCNNGG 1 cut(s) 263
BsaXI ACNNNNNCTCC 2 cut(s) 494, 524
Bsc4I CCNNNNNNNGG 1 cut(s) 516
Bse1I ACTGG 1 cut(s) 528
Bse3DI GCAATG 1 cut(s) 418
Bse8I GATNNNNATC 1 cut(s) 555
BseCI ATCGAT 2 cut(s) 559, 563
BseDI CCNNGG 1 cut(s) 263
BseGI GGATG 2 cut(s) 192, 580
BseJI GATNNNNATC 1 cut(s) 555
BseLI CCNNNNNNNGG 1 cut(s) 516
BseMI GCAATG 1 cut(s) 418
BseNI ACTGG 1 cut(s) 528
BseRI GAGGAG 3 cut(s) 117, 120, 307
BseXI GCAGC 1 cut(s) 449
Bsh1285I CGRYCG 2 cut(s) 563, 567
BshFI GGCC 2 cut(s) 283, 353
BshVI ATCGAT 2 cut(s) 559, 563
BsiEI CGRYCG 2 cut(s) 563, 567
BsiHKAI GWGCWC 1 cut(s) 361
BsiSI CCGG 1 cut(s) 350
BslFI GGGAC 2 cut(s) 432, 476
BslI CCNNNNNNNGG 1 cut(s) 516
BsmFI GGGAC 2 cut(s) 432, 476
BsnI GGCC 2 cut(s) 283, 353
Bsp1286I GDGCHC 1 cut(s) 361
Bsp143I GATC 5 cut(s) 189, 523, 556, 560, 564
BspACI CCGC 8 cut(s) 12, 96, 99, 143, 306, 309, 312, 365
BspANI GGCC 2 cut(s) 283, 353
BspDI ATCGAT 2 cut(s) 559, 563
BspLI GGNNCC 1 cut(s) 261
BspPI GGATC 3 cut(s) 184, 518, 564
BspQI GCTCTTC 1 cut(s) 366
BsrDI GCAATG 1 cut(s) 418
BsrI ACTGG 1 cut(s) 528
BssECI CCNNGG 1 cut(s) 263
BssMI GATC 5 cut(s) 189, 523, 556, 560, 564
BssNI GRCGYC 1 cut(s) 146
BssT1I CCWWGG 1 cut(s) 263
Bst6I CTCTTC 3 cut(s) 10, 351, 366
BstACI GRCGYC 1 cut(s) 146
BstC8I GCNNGC 1 cut(s) 285
BstEII GGTNACC 1 cut(s) 392
BstF5I GGATG 2 cut(s) 192, 580
BstKTI GATC 5 cut(s) 192, 526, 559, 563, 567
BstMBI GATC 5 cut(s) 189, 523, 556, 560, 564
BstMCI CGRYCG 2 cut(s) 563, 567
BstPI GGTNACC 1 cut(s) 392
BstV1I GCAGC 1 cut(s) 449
Bsu15I ATCGAT 2 cut(s) 559, 563
BsuRI GGCC 2 cut(s) 283, 353
BsuTUI ATCGAT 2 cut(s) 559, 563
BtrI CACGTC 1 cut(s) 222
BtsCI GGATG 2 cut(s) 192, 580
BtsIMutI CAGTG 1 cut(s) 535
Cac8I GCNNGC 1 cut(s) 285
Cfr13I GGNCC 1 cut(s) 260
ClaI ATCGAT 2 cut(s) 559, 563
CseI GACGC 1 cut(s) 135
CviAII CATG 2 cut(s) 408, 451
CviJI RGCY 6 cut(s) 181, 242, 283, 353, 359, 521
CviKI_1 RGCY 6 cut(s) 181, 242, 283, 353, 359, 521
DpnI GATC 5 cut(s) 191, 525, 558, 562, 566
DpnII GATC 5 cut(s) 189, 523, 556, 560, 564
EaeI YGGCCR 1 cut(s) 351
Eam1104I CTCTTC 3 cut(s) 10, 351, 366
EarI CTCTTC 3 cut(s) 10, 351, 366
EciI GGCGGA 4 cut(s) 27, 114, 295, 354
Ecl136II GAGCTC 1 cut(s) 359
Eco130I CCWWGG 1 cut(s) 263
Eco24I GRGCYC 1 cut(s) 361
Eco47I GGWCC 1 cut(s) 260
Eco53kI GAGCTC 1 cut(s) 359
Eco91I GGTNACC 1 cut(s) 392
EcoICRI GAGCTC 1 cut(s) 359
EcoO109I RGGNCCY 1 cut(s) 260
EcoO65I GGTNACC 1 cut(s) 392
EcoRI GAATTC 1 cut(s) 533
EcoT14I CCWWGG 1 cut(s) 263
EcoT38I GRGCYC 1 cut(s) 361
ErhI CCWWGG 1 cut(s) 263
FaeI CATG 2 cut(s) 411, 454
FaiI YATR 8 cut(s) 26, 254, 381, 409, 445, 452, 488, 545
FaqI GGGAC 2 cut(s) 432, 476
FatI CATG 2 cut(s) 407, 450
Fnu4HI GCNGC 5 cut(s) 97, 144, 309, 312, 438
FokI GGATG 2 cut(s) 199, 567
FriOI GRGCYC 1 cut(s) 361
Fsp4HI GCNGC 5 cut(s) 97, 144, 309, 312, 438
FspBI CTAG 1 cut(s) 264
GluI GCNGC 5 cut(s) 97, 144, 309, 312, 438
GsuI CTGGAG 1 cut(s) 320
HaeIII GGCC 2 cut(s) 283, 353
HapII CCGG 1 cut(s) 350
HgaI GACGC 1 cut(s) 135
Hin1I GRCGYC 1 cut(s) 146
Hin1II CATG 2 cut(s) 411, 454
HinfI GANTC 2 cut(s) 302, 420
HpaII CCGG 1 cut(s) 350
Hpy166II GTNNAC 1 cut(s) 219
Hpy188III TCNNGA 1 cut(s) 299
Hpy8I GTNNAC 1 cut(s) 219
Hpy99I CGWCG 5 cut(s) 151, 154, 160, 166, 208
HpyAV CCTTC 2 cut(s) 49, 351
HpyCH4IV ACGT 2 cut(s) 221, 477
HpyCH4V TGCA 5 cut(s) 42, 225, 232, 328, 411
HpySE526I ACGT 2 cut(s) 221, 477
Hsp92I GRCGYC 1 cut(s) 146
Hsp92II CATG 2 cut(s) 411, 454
Kzo9I GATC 5 cut(s) 189, 523, 556, 560, 564
LguI GCTCTTC 1 cut(s) 366
Lsp1109I GCAGC 1 cut(s) 449
LweI GCATC 2 cut(s) 120, 234
MaeI CTAG 1 cut(s) 264
MaeII ACGT 2 cut(s) 221, 477
MaeIII GTNAC 4 cut(s) 136, 392, 446, 478
MalI GATC 5 cut(s) 191, 525, 558, 562, 566
MboI GATC 5 cut(s) 189, 523, 556, 560, 564
MboII GAAGA 4 cut(s) 27, 64, 338, 353
MhlI GDGCHC 1 cut(s) 361
MluCI AATT 2 cut(s) 233, 533
MlyI GAGTC 2 cut(s) 311, 414
MnlI CCTC 9 cut(s) 86, 95, 98, 101, 159, 325, 328, 366, 520
MseI TTAA 3 cut(s) 81, 119, 236
MspI CCGG 1 cut(s) 350
NdeII GATC 5 cut(s) 189, 523, 556, 560, 564
NlaIII CATG 2 cut(s) 411, 454
NlaIV GGNNCC 1 cut(s) 261
NmuCI GTSAC 3 cut(s) 136, 446, 478
PciSI GCTCTTC 1 cut(s) 366
PcsI WCGNNNNNNNCGW 2 cut(s) 146, 158
PflFI GACNNNGTC 1 cut(s) 202
PkrI GCNGC 5 cut(s) 98, 145, 310, 313, 439
Ple19I CGATCG 2 cut(s) 563, 567
PleI GAGTC 2 cut(s) 310, 414
PpsI GAGTC 2 cut(s) 310, 414
PpuMI RGGWCCY 1 cut(s) 260
PsiI TTATAA 1 cut(s) 381
Psp124BI GAGCTC 1 cut(s) 361
Psp5II RGGWCCY 1 cut(s) 260
PspEI GGTNACC 1 cut(s) 392
PspN4I GGNNCC 1 cut(s) 261
PspPI GGNCC 1 cut(s) 260
PspPPI RGGWCCY 1 cut(s) 260
PsyI GACNNNGTC 1 cut(s) 202
PvuI CGATCG 2 cut(s) 563, 567
SacI GAGCTC 1 cut(s) 361
SapI GCTCTTC 1 cut(s) 366
SaqAI TTAA 3 cut(s) 81, 119, 236
SatI GCNGC 5 cut(s) 97, 144, 309, 312, 438
Sau3AI GATC 5 cut(s) 189, 523, 556, 560, 564
Sau96I GGNCC 1 cut(s) 260
SchI GAGTC 2 cut(s) 311, 414
SduI GDGCHC 1 cut(s) 361
SfaNI GCATC 2 cut(s) 120, 234
SinI GGWCC 1 cut(s) 260
Sse9I AATT 2 cut(s) 233, 533
SsiI CCGC 8 cut(s) 12, 96, 99, 143, 306, 309, 312, 365
SspMI CTAG 1 cut(s) 264
SstI GAGCTC 1 cut(s) 361
StyI CCWWGG 1 cut(s) 263
TaiI ACGT 2 cut(s) 224, 480
TaqI TCGA 6 cut(s) 128, 164, 170, 501, 559, 563
TasI AATT 2 cut(s) 233, 533
TauI GCSGC 4 cut(s) 99, 146, 311, 314
Tru1I TTAA 3 cut(s) 81, 119, 236
Tru9I TTAA 3 cut(s) 81, 119, 236
TscAI CASTG 1 cut(s) 535
TseFI GTSAC 3 cut(s) 136, 446, 478
TseI GCWGC 1 cut(s) 437
Tsp45I GTSAC 3 cut(s) 136, 446, 478
TspDTI ATGAA 2 cut(s) 191, 564
TspGWI ACGGA 2 cut(s) 182, 310
TspRI CASTG 1 cut(s) 535
Tth111I GACNNNGTC 1 cut(s) 202
VpaK11BI GGWCC 1 cut(s) 260
XapI RAATTY 1 cut(s) 533
XmaJI CCTAGG 1 cut(s) 263
XspI CTAG 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.