pycom06g20970

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0029311
Physical Location & Seq
Forward (+)
23822033 .. 23823183
1151 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g20970.1

Sequence Viewer

Length: 858 bp
ATGTGGAAAGATTGTTCTGTCAAGACCGTGTTTCCTTTCTCCAAGGGTGATGTGTCAACGAGTTATGATAGTGCTTCTGGTTCTGACAAAAAAGAAGGAAAGAGAAAAACTGAGTTGACTGATATTAGGGCAACAATAAAGGAGCAGGTTGATGAGGTGGGAAGAGCTTTATACTTAAGCCAGTCACAAGGGAGTTCTGAGCAAAAGACCATTAGTAATTTAGTTTTTCCTATTTTGTCCGAGAATCAAAAGGAAGATTTCCCTAGGCTGCCACCAGTCAAACTCAAGTCAGAGGACAGACCATTGAATGTTAATTGGGAGGAAAAGTTTGAACGAGACGGACCTGGCACGAAACTCAGCTCTGCTGACAATGCTCTTATTGGGTCTTATTTGGATGTACCTATTGGACAAGAAATCAACTCTTCAGATTATGGTTCAGGTGGAAAAAGACCTGTAGGAGGCAGTTGGCTGTCTGTAAGTCAGGGGATCGCAGAGGACGCATCTGATCTGGTTTCTGGTTTTGCTACCGTTGGTGATGGATTGAGTGAAACGATTGACTACCCATATTGGGACTCTGATGAATATGATGATGATGATGATGTCGGATACATGAGACAACCTATTGAAGATGAGTCATGGTTTCTGGCCCATGAAATTGATTATCCTAGTGACAATGAAAAGGGAACAGGTCATGGGAGTGTTCCAGATCCACAGGAAAGGGGCCCAACCAAAGAGGAGGATGATGATCAATCTTTTGCTGAGGAGGATTCTTATTTATCTGGTGAGCGATACTTTCAAGCGAAAAATGTTGAACCAATTATAACTTCAGATCGATCCTATGGGATTATCAGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

286

Amino Acids

31.58

Weight (kDa)

4.29

Isoelectric Point (pI)

46.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029311)

Species Orthologous Gene IDs
pyrus_communis pycom06g20970 pycom14g20340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 821
Acc36I ACCTGC 1 cut(s) 136
AclWI GGATC 3 cut(s) 494, 701, 828
AcuI CTGAAG 2 cut(s) 408, 810
AfaI GTAC 1 cut(s) 399
AfiI CCNNNNNNNGG 2 cut(s) 458, 568
AflII CTTAAG 1 cut(s) 175
AgsI TTSAA 5 cut(s) 307, 332, 626, 797, 812
AjnI CCWGG 1 cut(s) 343
AjuI GAANNNNNNNTTGG 2 cut(s) 808, 840
AloI GAACNNNNNNTCC 1 cut(s) 30
AluBI AGCT 2 cut(s) 167, 360
AluI AGCT 2 cut(s) 167, 360
Alw26I GTCTC 2 cut(s) 330, 607
AlwI GGATC 3 cut(s) 494, 701, 828
AlwNI CAGNNNCTG 1 cut(s) 855
AoxI GGCC 2 cut(s) 645, 721
ApaI GGGCCC 1 cut(s) 725
ApeKI GCWGC 1 cut(s) 268
AspA2I CCTAGG 1 cut(s) 263
AspS9I GGNCC 4 cut(s) 341, 646, 721, 722
AsuHPI GGTGA 3 cut(s) 59, 545, 794
AvaII GGWCC 1 cut(s) 341
AvrII CCTAGG 1 cut(s) 263
BaeGI GKGCMC 1 cut(s) 725
BanII GRGCYC 1 cut(s) 725
BbvCI CCTCAGC 1 cut(s) 759
BbvI GCAGC 1 cut(s) 255
BccI CCATC 1 cut(s) 530
BciT130I CCWGG 1 cut(s) 345
BciVI GTATCC 1 cut(s) 599
BclI TGATCA 1 cut(s) 745
BcoDI GTCTC 2 cut(s) 330, 607
BfaI CTAG 2 cut(s) 264, 666
BfmI CTRYAG 1 cut(s) 453
BfrI CTTAAG 1 cut(s) 175
BfuAI ACCTGC 1 cut(s) 136
BfuI GTATCC 1 cut(s) 599
BisI GCNGC 1 cut(s) 269
BlnI CCTAGG 1 cut(s) 263
BlsI GCNGC 1 cut(s) 270
Bme1390I CCNGG 1 cut(s) 345
Bme18I GGWCC 1 cut(s) 341
BmgT120I GGNCC 4 cut(s) 341, 646, 721, 722
BmiI GGNNCC 2 cut(s) 722, 723
BmrFI CCNGG 1 cut(s) 345
BmsI GCATC 1 cut(s) 509
Bpu10I CCTNAGC 1 cut(s) 759
BpuEI CTTGAG 1 cut(s) 269
Bsa29I ATCGAT 1 cut(s) 832
BsaBI GATNNNNATC 1 cut(s) 744
BsaJI CCNNGG 2 cut(s) 42, 263
BsaXI ACNNNNNCTCC 2 cut(s) 311, 341
Bsc4I CCNNNNNNNGG 2 cut(s) 458, 568
Bse1I ACTGG 2 cut(s) 181, 275
Bse8I GATNNNNATC 1 cut(s) 744
BseBI CCWGG 1 cut(s) 345
BseCI ATCGAT 1 cut(s) 832
BseDI CCNNGG 2 cut(s) 42, 263
BseGI GGATG 2 cut(s) 400, 745
BseJI GATNNNNATC 1 cut(s) 744
BseLI CCNNNNNNNGG 2 cut(s) 458, 568
BseMII CTCAG 4 cut(s) 102, 189, 370, 750
BseNI ACTGG 2 cut(s) 181, 275
BseRI GAGGAG 2 cut(s) 749, 776
BseSI GKGCMC 1 cut(s) 725
BseXI GCAGC 1 cut(s) 255
BshFI GGCC 2 cut(s) 647, 723
BshVI ATCGAT 1 cut(s) 832
BslFI GGGAC 1 cut(s) 584
BslI CCNNNNNNNGG 2 cut(s) 458, 568
BsmAI GTCTC 2 cut(s) 330, 607
BsmBI CGTCTC 1 cut(s) 330
BsmFI GGGAC 1 cut(s) 584
BsnI GGCC 2 cut(s) 647, 723
Bsp120I GGGCCC 1 cut(s) 721
Bsp1286I GDGCHC 1 cut(s) 725
Bsp143I GATC 6 cut(s) 486, 505, 706, 745, 829, 833
BspANI GGCC 2 cut(s) 647, 723
BspCNI CTCAG 4 cut(s) 103, 190, 369, 751
BspDI ATCGAT 1 cut(s) 832
BspLI GGNNCC 2 cut(s) 722, 723
BspMI ACCTGC 1 cut(s) 136
BspPI GGATC 3 cut(s) 494, 701, 828
BspQI GCTCTTC 1 cut(s) 157
BspTI CTTAAG 1 cut(s) 175
BsrI ACTGG 2 cut(s) 181, 275
BssECI CCNNGG 2 cut(s) 42, 263
BssMI GATC 6 cut(s) 486, 505, 706, 745, 829, 833
BssT1I CCWWGG 2 cut(s) 42, 263
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 2 cut(s) 28, 529
Bst6I CTCTTC 2 cut(s) 157, 427
BstAFI CTTAAG 1 cut(s) 175
BstDEI CTNAG 4 cut(s) 111, 198, 356, 759
BstENI CCTNNNNNAGG 1 cut(s) 456
BstF5I GGATG 2 cut(s) 400, 745
BstKTI GATC 6 cut(s) 489, 508, 709, 748, 832, 836
BstMAI GTCTC 2 cut(s) 330, 607
BstMBI GATC 6 cut(s) 486, 505, 706, 745, 829, 833
BstMWI GCNNNNNNNGC 2 cut(s) 371, 497
BstNI CCWGG 1 cut(s) 345
BstSCI CCNGG 1 cut(s) 343
BstSFI CTRYAG 1 cut(s) 453
BstSLI GKGCMC 1 cut(s) 725
BstV1I GCAGC 1 cut(s) 255
BstX2I RGATCY 1 cut(s) 706
BstYI RGATCY 1 cut(s) 706
Bsu15I ATCGAT 1 cut(s) 832
BsuI GTATCC 1 cut(s) 599
BsuRI GGCC 2 cut(s) 647, 723
BsuTUI ATCGAT 1 cut(s) 832
BtsCI GGATG 2 cut(s) 400, 745
BtsIMutI CAGTG 1 cut(s) 856
BveI ACCTGC 1 cut(s) 136
CaiI CAGNNNCTG 1 cut(s) 855
Cfr13I GGNCC 4 cut(s) 341, 646, 721, 722
ClaI ATCGAT 1 cut(s) 832
CseI GACGC 1 cut(s) 506
Csp6I GTAC 1 cut(s) 398
CviAII CATG 4 cut(s) 610, 636, 650, 692
CviJI RGCY 7 cut(s) 167, 180, 268, 360, 469, 647, 723
CviKI_1 RGCY 7 cut(s) 167, 180, 268, 360, 469, 647, 723
CviQI GTAC 1 cut(s) 398
DdeI CTNAG 4 cut(s) 111, 198, 356, 759
DpnI GATC 6 cut(s) 488, 507, 708, 747, 831, 835
DpnII GATC 6 cut(s) 486, 505, 706, 745, 829, 833
Eam1104I CTCTTC 2 cut(s) 157, 427
EarI CTCTTC 2 cut(s) 157, 427
Eco130I CCWWGG 2 cut(s) 42, 263
Eco24I GRGCYC 1 cut(s) 725
Eco47I GGWCC 1 cut(s) 341
Eco57I CTGAAG 2 cut(s) 408, 810
EcoNI CCTNNNNNAGG 1 cut(s) 456
EcoO109I RGGNCCY 1 cut(s) 721
EcoRII CCWGG 1 cut(s) 343
EcoT14I CCWWGG 2 cut(s) 42, 263
EcoT38I GRGCYC 1 cut(s) 725
ErhI CCWWGG 2 cut(s) 42, 263
Esp3I CGTCTC 1 cut(s) 330
FaeI CATG 4 cut(s) 613, 639, 653, 695
FaqI GGGAC 1 cut(s) 584
FatI CATG 4 cut(s) 609, 635, 649, 691
FbaI TGATCA 1 cut(s) 745
Fnu4HI GCNGC 1 cut(s) 269
FokI GGATG 2 cut(s) 407, 752
FriOI GRGCYC 1 cut(s) 725
Fsp4HI GCNGC 1 cut(s) 269
FspBI CTAG 2 cut(s) 264, 666
GluI GCNGC 1 cut(s) 269
HaeIII GGCC 2 cut(s) 647, 723
HgaI GACGC 1 cut(s) 506
Hin1II CATG 4 cut(s) 613, 639, 653, 695
HincII GTYRAC 2 cut(s) 57, 117
HindII GTYRAC 2 cut(s) 57, 117
HinfI GANTC 4 cut(s) 244, 572, 632, 767
HphI GGTGA 3 cut(s) 59, 545, 794
Hpy166II GTNNAC 2 cut(s) 57, 117
Hpy188I TCNGA 9 cut(s) 85, 199, 241, 292, 427, 505, 577, 605, 829
Hpy188III TCNNGA 2 cut(s) 22, 704
Hpy8I GTNNAC 2 cut(s) 57, 117
HpyAV CCTTC 1 cut(s) 89
HpyCH4III ACNGT 2 cut(s) 28, 529
HpyF10VI GCNNNNNNNGC 2 cut(s) 371, 497
HpyF3I CTNAG 4 cut(s) 111, 198, 356, 759
Hsp92II CATG 4 cut(s) 613, 639, 653, 695
Ksp22I TGATCA 1 cut(s) 745
Kzo9I GATC 6 cut(s) 486, 505, 706, 745, 829, 833
LguI GCTCTTC 1 cut(s) 157
LmnI GCTCC 1 cut(s) 142
Lsp1109I GCAGC 1 cut(s) 255
LweI GCATC 1 cut(s) 509
MaeI CTAG 2 cut(s) 264, 666
MaeIII GTNAC 3 cut(s) 183, 668, 851
MalI GATC 6 cut(s) 488, 507, 708, 747, 831, 835
MboI GATC 6 cut(s) 486, 505, 706, 745, 829, 833
MboII GAAGA 4 cut(s) 174, 266, 414, 638
MflI RGATCY 1 cut(s) 706
MhlI GDGCHC 1 cut(s) 725
MluCI AATT 4 cut(s) 217, 313, 654, 816
MlyI GAGTC 2 cut(s) 566, 641
MmeI TCCRAC 1 cut(s) 583
MnlI CCTC 9 cut(s) 148, 286, 313, 452, 487, 727, 730, 754, 757
MseI TTAA 2 cut(s) 176, 312
MslI CAYNNNNRTG 1 cut(s) 696
MspCI CTTAAG 1 cut(s) 175
MspR9I CCNGG 1 cut(s) 345
MvaI CCWGG 1 cut(s) 345
MwoI GCNNNNNNNGC 2 cut(s) 371, 497
NdeII GATC 6 cut(s) 486, 505, 706, 745, 829, 833
NlaIII CATG 4 cut(s) 613, 639, 653, 695
NlaIV GGNNCC 2 cut(s) 722, 723
NmuCI GTSAC 3 cut(s) 183, 668, 851
PciSI GCTCTTC 1 cut(s) 157
PfeI GAWTC 2 cut(s) 244, 767
PkrI GCNGC 1 cut(s) 270
PleI GAGTC 2 cut(s) 566, 640
PpsI GAGTC 2 cut(s) 566, 640
PsiI TTATAA 1 cut(s) 821
Psp6I CCWGG 1 cut(s) 343
PspGI CCWGG 1 cut(s) 343
PspN4I GGNNCC 2 cut(s) 722, 723
PspOMI GGGCCC 1 cut(s) 721
PspPI GGNCC 4 cut(s) 341, 646, 721, 722
PstNI CAGNNNCTG 1 cut(s) 855
PsuI RGATCY 1 cut(s) 706
RsaI GTAC 1 cut(s) 399
RsaNI GTAC 1 cut(s) 398
RseI CAYNNNNRTG 1 cut(s) 696
SapI GCTCTTC 1 cut(s) 157
SaqAI TTAA 2 cut(s) 176, 312
SatI GCNGC 1 cut(s) 269
Sau3AI GATC 6 cut(s) 486, 505, 706, 745, 829, 833
Sau96I GGNCC 4 cut(s) 341, 646, 721, 722
SchI GAGTC 2 cut(s) 566, 641
ScrFI CCNGG 1 cut(s) 345
SduI GDGCHC 1 cut(s) 725
SfaNI GCATC 1 cut(s) 509
SfcI CTRYAG 1 cut(s) 453
SinI GGWCC 1 cut(s) 341
SmiMI CAYNNNNRTG 1 cut(s) 696
SmlI CTYRAG 2 cut(s) 175, 284
SmoI CTYRAG 2 cut(s) 175, 284
Sse9I AATT 4 cut(s) 217, 313, 654, 816
SspMI CTAG 2 cut(s) 264, 666
StyD4I CCNGG 1 cut(s) 343
StyI CCWWGG 2 cut(s) 42, 263
TaaI ACNGT 2 cut(s) 28, 529
TaqI TCGA 1 cut(s) 832
TasI AATT 4 cut(s) 217, 313, 654, 816
TfiI GAWTC 2 cut(s) 244, 767
Tru1I TTAA 2 cut(s) 176, 312
Tru9I TTAA 2 cut(s) 176, 312
TscAI CASTG 1 cut(s) 856
TseFI GTSAC 3 cut(s) 183, 668, 851
TseI GCWGC 1 cut(s) 268
Tsp45I GTSAC 3 cut(s) 183, 668, 851
TspDTI ATGAA 3 cut(s) 594, 666, 690
TspGWI ACGGA 1 cut(s) 354
TspRI CASTG 1 cut(s) 856
Vha464I CTTAAG 1 cut(s) 175
VpaK11BI GGWCC 1 cut(s) 341
XagI CCTNNNNNAGG 1 cut(s) 456
XmaJI CCTAGG 1 cut(s) 263
XspI CTAG 2 cut(s) 264, 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.