pycom07g03040

ATP-dependent Clp protease proteolytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0029312
Physical Location & Seq
Forward (+)
2501786 .. 2502196
411 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 330 bp
ATGGTGTACCACAAGATTAAATTGAACAAAATATTCTCAAGAATCACGGGGAAGCCTTTGGAGCAGATTGAAGAGGACACGGACCGTGATAATTTCATGAATCCTTGGGAAGCTAGGGATTATGGGTTGATTGATGTCGTTATCGATGATGGAAAGCCAGGCTTAGTTGCACCCATTGGAGATTTCACACCTCCACCAAGAACACGAGTCTGGGATCAGTGGAAAGTTGAAGGGAGCAAGAAATCGAGAAAGAACTTGCCCTCAGAACAAAGGATTTCTGACACTGAAAGAGGGGACGAGAGGGAAAAGGAAGCACCCGCTGCTATATGA

Protein Analysis

110

Amino Acids

12.58

Weight (kDa)

5.48

Isoelectric Point (pI)

30.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 3 - 49 5.2e-13 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0029312)

Species Orthologous Gene IDs
pyrus_communis pycom07g03040
rosa_multiflora Rmu_co8375257.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 318
AclWI GGATC 1 cut(s) 222
AfaI GTAC 1 cut(s) 8
AgsI TTSAA 3 cut(s) 25, 71, 230
AjnI CCWGG 1 cut(s) 157
AluBI AGCT 1 cut(s) 113
AluI AGCT 1 cut(s) 113
AlwI GGATC 1 cut(s) 222
ApeKI GCWGC 1 cut(s) 320
AspS9I GGNCC 1 cut(s) 82
AvaII GGWCC 1 cut(s) 82
BauI CACGAG 1 cut(s) 204
BbvI GCAGC 1 cut(s) 307
BccI CCATC 1 cut(s) 143
BciT130I CCWGG 1 cut(s) 159
BfaI CTAG 1 cut(s) 114
BisI GCNGC 1 cut(s) 321
BlsI GCNGC 1 cut(s) 322
Bme1390I CCNGG 1 cut(s) 159
Bme18I GGWCC 1 cut(s) 82
BmgT120I GGNCC 1 cut(s) 82
BmrFI CCNGG 1 cut(s) 159
BpuEI CTTGAG 1 cut(s) 22
Bsa29I ATCGAT 1 cut(s) 144
BsaJI CCNNGG 1 cut(s) 104
BseBI CCWGG 1 cut(s) 159
BseCI ATCGAT 1 cut(s) 144
BseDI CCNNGG 1 cut(s) 104
BseMII CTCAG 1 cut(s) 276
BseXI GCAGC 1 cut(s) 307
BshVI ATCGAT 1 cut(s) 144
BslFI GGGAC 1 cut(s) 308
BsmFI GGGAC 1 cut(s) 308
Bsp143I GATC 1 cut(s) 214
BspACI CCGC 1 cut(s) 318
BspCNI CTCAG 1 cut(s) 275
BspDI ATCGAT 1 cut(s) 144
BspHI TCATGA 1 cut(s) 96
BspPI GGATC 1 cut(s) 222
BssECI CCNNGG 1 cut(s) 104
BssMI GATC 1 cut(s) 214
BssSI CACGAG 1 cut(s) 204
BssT1I CCWWGG 1 cut(s) 104
Bst2BI CACGAG 1 cut(s) 204
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 1 cut(s) 86
Bst6I CTCTTC 1 cut(s) 66
BstAPI GCANNNNNTGC 1 cut(s) 320
BstDEI CTNAG 2 cut(s) 163, 262
BstKTI GATC 1 cut(s) 217
BstMBI GATC 1 cut(s) 214
BstMWI GCNNNNNNNGC 2 cut(s) 61, 320
BstNI CCWGG 1 cut(s) 159
BstSCI CCNGG 1 cut(s) 157
BstV1I GCAGC 1 cut(s) 307
Bsu15I ATCGAT 1 cut(s) 144
BsuTUI ATCGAT 1 cut(s) 144
BtsIMutI CAGTG 2 cut(s) 224, 282
CciI TCATGA 1 cut(s) 96
Cfr13I GGNCC 1 cut(s) 82
ClaI ATCGAT 1 cut(s) 144
CpoI CGGWCCG 1 cut(s) 82
Csp6I GTAC 1 cut(s) 7
CspI CGGWCCG 1 cut(s) 82
CviAII CATG 1 cut(s) 97
CviJI RGCY 4 cut(s) 55, 113, 157, 162
CviKI_1 RGCY 4 cut(s) 55, 113, 157, 162
CviQI GTAC 1 cut(s) 7
DdeI CTNAG 2 cut(s) 163, 262
DpnI GATC 1 cut(s) 216
DpnII GATC 1 cut(s) 214
Eam1104I CTCTTC 1 cut(s) 66
EarI CTCTTC 1 cut(s) 66
Eco130I CCWWGG 1 cut(s) 104
Eco47I GGWCC 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 157
EcoT14I CCWWGG 1 cut(s) 104
ErhI CCWWGG 1 cut(s) 104
FaeI CATG 1 cut(s) 100
FaiI YATR 4 cut(s) 98, 123, 326, 328
FalI AAGNNNNNCTT 2 cut(s) 146, 178
FaqI GGGAC 1 cut(s) 308
FatI CATG 1 cut(s) 96
FauI CCCGC 1 cut(s) 325
Fnu4HI GCNGC 1 cut(s) 321
Fsp4HI GCNGC 1 cut(s) 321
FspBI CTAG 1 cut(s) 114
GluI GCNGC 1 cut(s) 321
Hin1II CATG 1 cut(s) 100
HinfI GANTC 3 cut(s) 42, 100, 207
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 2 cut(s) 265, 280
Hpy188III TCNNGA 3 cut(s) 39, 97, 246
Hpy8I GTNNAC 1 cut(s) 7
HpyAV CCTTC 1 cut(s) 224
HpyCH4III ACNGT 1 cut(s) 86
HpyCH4V TGCA 1 cut(s) 170
HpyF10VI GCNNNNNNNGC 2 cut(s) 61, 320
HpyF3I CTNAG 2 cut(s) 163, 262
Hsp92II CATG 1 cut(s) 100
Kzo9I GATC 1 cut(s) 214
LmnI GCTCC 2 cut(s) 61, 234
LpnPI CCDG 3 cut(s) 144, 171, 196
Lsp1109I GCAGC 1 cut(s) 307
MaeI CTAG 1 cut(s) 114
MalI GATC 1 cut(s) 216
MboI GATC 1 cut(s) 214
MboII GAAGA 1 cut(s) 83
MluCI AATT 2 cut(s) 20, 91
MlyI GAGTC 1 cut(s) 216
MnlI CCTC 5 cut(s) 67, 201, 271, 284, 294
MseI TTAA 1 cut(s) 18
MspA1I CMGCKG 1 cut(s) 320
MspR9I CCNGG 1 cut(s) 159
MvaI CCWGG 1 cut(s) 159
MwoI GCNNNNNNNGC 2 cut(s) 61, 320
NdeII GATC 1 cut(s) 214
NlaIII CATG 1 cut(s) 100
PagI TCATGA 1 cut(s) 96
PfeI GAWTC 2 cut(s) 42, 100
PkrI GCNGC 1 cut(s) 322
PleI GAGTC 1 cut(s) 215
PpsI GAGTC 1 cut(s) 215
Psp6I CCWGG 1 cut(s) 157
PspGI CCWGG 1 cut(s) 157
PspPI GGNCC 1 cut(s) 82
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
Rsr2I CGGWCCG 1 cut(s) 82
RsrII CGGWCCG 1 cut(s) 82
SaqAI TTAA 1 cut(s) 18
SatI GCNGC 1 cut(s) 321
Sau3AI GATC 1 cut(s) 214
Sau96I GGNCC 1 cut(s) 82
SchI GAGTC 1 cut(s) 216
ScrFI CCNGG 1 cut(s) 159
SetI ASST 2 cut(s) 115, 193
SinI GGWCC 1 cut(s) 82
SmlI CTYRAG 1 cut(s) 37
SmoI CTYRAG 1 cut(s) 37
Sse9I AATT 2 cut(s) 20, 91
SsiI CCGC 1 cut(s) 318
SspI AATATT 1 cut(s) 33
SspMI CTAG 1 cut(s) 114
StyD4I CCNGG 1 cut(s) 157
StyI CCWWGG 1 cut(s) 104
TaaI ACNGT 1 cut(s) 86
TaqI TCGA 2 cut(s) 144, 245
TasI AATT 2 cut(s) 20, 91
TfiI GAWTC 2 cut(s) 42, 100
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TscAI CASTG 2 cut(s) 224, 289
TseI GCWGC 1 cut(s) 320
TspDTI ATGAA 2 cut(s) 85, 113
TspGWI ACGGA 1 cut(s) 95
TspRI CASTG 2 cut(s) 224, 289
VpaK11BI GGWCC 1 cut(s) 82
XspI CTAG 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.