pycom07g11760

Retrovirus-related Pol polyprotein from transposon TNT 1-94

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0021251
Physical Location & Seq
Reverse (-)
12928659 .. 12929522
864 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 864 bp
ATGCCTAAAATACTACCCACCATTATACTTGACAAAATGGCCCAAGACTTTTTCCTTGCATATCTCTCGGATGATTTCACTAAGTTTATTGTAAACTATAAAGTGAATAAATTCGATCATACTTTTAACGAGATGATTGACATATGCTGTAAATTTGAAAAATGTTTCAAAAAGGACAGTGGGAGTGAGAATGCAAAAACAAGGAAAAGGTTACTTAAGAAGAAGGCAAAGAAATCCAAAGGAACATGCTTTCATTGTGGAAAGGATGAACGTTGGAAGAAGAAATACAGGGTGCGCATTGCGAGCCTTATGACACGAACTTTTGAAGAGACTATTTCGGTCATAGAAAGTGCTTTTACAGTGAGCTCCAATTCCTGGATATTTGATTCAGGCGCTAGTCAACATATCTGTAATACGATGCAGGGACTAGTAGGGAGCAAGACACTACGCAATGGGGAGATGGTTGTGCGAGTTGGGAACGGCGCTAAAATCTCTGCAAAAGCAATAGGCACCTACATGCTTAACCTACCCTCTGGGGAAGTCCTGGAACTTAAAAATTGTTTATATTTTCCTTCATGTATAAAGAATTTGATTTCTATCTCTAAGCTTTTACGAGATGGGCACTCAGTATTGTTTGACAAAATGAGTTGCACTTTATACTTGAACGGTCGTATTATCTCTCATGGTAATATGATAGAGGGACTTTTTCACCTAGAGACGAATAGTGGGATGCACTGTATTGAAAGCGGGAATACCTCAAAACCCAAAAGGGCTAGAGAAGAAGTTAACCAAGTTAGACTTTGGCATCTTAAACTTGGACATGTAAACCAGGATAAGATTCGCAAGATGTCGAAAGATGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2 gag_pre-integrs

Protein Analysis

288

Amino Acids

32.67

Weight (kDa)

9.58

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 126 - 207 2.5e-15 Pol polyprotein, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0021251)

Species Orthologous Gene IDs
pyrus_communis pycom01g00030 pycom03g10950 pycom06g06370 pycom06g08850 pycom07g11760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 296
AccB1I GGYRCC 1 cut(s) 509
AccB7I CCANNNNNTGG 1 cut(s) 375
AciI CCGC 1 cut(s) 747
AclI AACGTT 1 cut(s) 271
AcsI RAATTY 3 cut(s) 110, 152, 586
AfiI CCNNNNNNNGG 1 cut(s) 375
AflII CTTAAG 1 cut(s) 215
AflIII ACRYGT 1 cut(s) 820
AgsI TTSAA 5 cut(s) 158, 169, 326, 664, 743
AhlI ACTAGT 1 cut(s) 427
AjnI CCWGG 3 cut(s) 374, 543, 828
AluBI AGCT 2 cut(s) 366, 607
AluI AGCT 2 cut(s) 366, 607
Alw21I GWGCWC 1 cut(s) 368
Alw26I GTCTC 2 cut(s) 323, 710
AoxI GGCC 1 cut(s) 39
ApoI RAATTY 3 cut(s) 110, 152, 586
Asp700I GAANNNNTTC 1 cut(s) 110
AspLEI GCGC 3 cut(s) 297, 395, 485
AspS9I GGNCC 1 cut(s) 40
AsuHPI GGTGA 1 cut(s) 701
BaeGI GKGCMC 1 cut(s) 624
BanI GGYRCC 1 cut(s) 509
BanII GRGCYC 1 cut(s) 368
BarI GAAGNNNNNNTAC 2 cut(s) 269, 301
Bbv12I GWGCWC 1 cut(s) 368
BccI CCATC 3 cut(s) 454, 611, 851
BceAI ACGGC 1 cut(s) 496
BcgI CGANNNNNNTGC 2 cut(s) 48, 82
BciT130I CCWGG 3 cut(s) 376, 545, 830
BcoDI GTCTC 2 cut(s) 323, 710
BcuI ACTAGT 1 cut(s) 427
BfaI CTAG 4 cut(s) 396, 428, 713, 774
BfoI RGCGCY 2 cut(s) 396, 486
BfrI CTTAAG 1 cut(s) 215
Bme1390I CCNGG 3 cut(s) 376, 545, 830
BmgT120I GGNCC 1 cut(s) 40
BmiI GGNNCC 1 cut(s) 511
BmrFI CCNGG 3 cut(s) 376, 545, 830
BmsI GCATC 3 cut(s) 408, 720, 814
BsaBI GATNNNNATC 1 cut(s) 596
Bsc4I CCNNNNNNNGG 1 cut(s) 375
Bse3DI GCAATG 2 cut(s) 297, 457
Bse8I GATNNNNATC 1 cut(s) 596
BseBI CCWGG 3 cut(s) 376, 545, 830
BseGI GGATG 3 cut(s) 76, 271, 735
BseJI GATNNNNATC 1 cut(s) 596
BseLI CCNNNNNNNGG 1 cut(s) 375
BseMI GCAATG 2 cut(s) 297, 457
BseMII CTCAG 1 cut(s) 639
BseSI GKGCMC 1 cut(s) 624
Bsh1285I CGRYCG 1 cut(s) 670
BshFI GGCC 1 cut(s) 41
BshNI GGYRCC 1 cut(s) 509
BsiEI CGRYCG 1 cut(s) 670
BsiHKAI GWGCWC 1 cut(s) 368
BslFI GGGAC 2 cut(s) 438, 714
BslI CCNNNNNNNGG 1 cut(s) 375
BsmAI GTCTC 2 cut(s) 323, 710
BsmBI CGTCTC 1 cut(s) 710
BsmFI GGGAC 2 cut(s) 438, 714
BsmI GAATGC 1 cut(s) 196
BsnI GGCC 1 cut(s) 41
Bsp1286I GDGCHC 2 cut(s) 368, 624
Bsp143I GATC 1 cut(s) 115
BspACI CCGC 1 cut(s) 747
BspANI GGCC 1 cut(s) 41
BspCNI CTCAG 1 cut(s) 638
BspLI GGNNCC 1 cut(s) 511
BspT107I GGYRCC 1 cut(s) 509
BspTI CTTAAG 1 cut(s) 215
BsrDI GCAATG 2 cut(s) 297, 457
BssMI GATC 1 cut(s) 115
Bst2UI CCWGG 3 cut(s) 376, 545, 830
Bst4CI ACNGT 4 cut(s) 179, 361, 668, 737
Bst6I CTCTTC 1 cut(s) 321
BstAFI CTTAAG 1 cut(s) 215
BstC8I GCNNGC 1 cut(s) 304
BstDEI CTNAG 3 cut(s) 81, 603, 625
BstF5I GGATG 3 cut(s) 76, 271, 735
BstH2I RGCGCY 2 cut(s) 396, 486
BstHHI GCGC 3 cut(s) 297, 395, 485
BstKTI GATC 1 cut(s) 118
BstMAI GTCTC 2 cut(s) 323, 710
BstMBI GATC 1 cut(s) 115
BstMCI CGRYCG 1 cut(s) 670
BstMWI GCNNNNNNNGC 1 cut(s) 303
BstNI CCWGG 3 cut(s) 376, 545, 830
BstNSI RCATGY 3 cut(s) 249, 520, 824
BstSCI CCNGG 3 cut(s) 374, 543, 828
BstSLI GKGCMC 1 cut(s) 624
BsuRI GGCC 1 cut(s) 41
BtsCI GGATG 3 cut(s) 76, 271, 735
BtsIMutI CAGTG 3 cut(s) 184, 366, 733
Cac8I GCNNGC 1 cut(s) 304
CfoI GCGC 3 cut(s) 297, 395, 485
Cfr13I GGNCC 1 cut(s) 40
CviAII CATG 5 cut(s) 246, 517, 576, 683, 821
CviJI RGCY 5 cut(s) 41, 306, 366, 607, 773
CviKI_1 RGCY 5 cut(s) 41, 306, 366, 607, 773
DdeI CTNAG 3 cut(s) 81, 603, 625
DpnI GATC 1 cut(s) 117
DpnII GATC 1 cut(s) 115
Eam1104I CTCTTC 1 cut(s) 321
EarI CTCTTC 1 cut(s) 321
Ecl136II GAGCTC 1 cut(s) 366
Eco24I GRGCYC 1 cut(s) 368
Eco53kI GAGCTC 1 cut(s) 366
EcoICRI GAGCTC 1 cut(s) 366
EcoRII CCWGG 3 cut(s) 374, 543, 828
EcoT38I GRGCYC 1 cut(s) 368
Esp3I CGTCTC 1 cut(s) 710
FaeI CATG 5 cut(s) 249, 520, 579, 686, 824
FalI AAGNNNNNCTT 2 cut(s) 783, 815
FaqI GGGAC 2 cut(s) 438, 714
FatI CATG 5 cut(s) 245, 516, 575, 682, 820
FauI CCCGC 1 cut(s) 740
FauNDI CATATG 1 cut(s) 143
FokI GGATG 3 cut(s) 83, 278, 742
FriOI GRGCYC 1 cut(s) 368
FspAI RTGCGCAY 1 cut(s) 296
FspBI CTAG 4 cut(s) 396, 428, 713, 774
FspI TGCGCA 1 cut(s) 296
GlaI GCGC 3 cut(s) 296, 394, 484
HaeII RGCGCY 2 cut(s) 396, 486
HaeIII GGCC 1 cut(s) 41
HhaI GCGC 3 cut(s) 297, 395, 485
Hin1II CATG 5 cut(s) 249, 520, 579, 686, 824
Hin6I GCGC 3 cut(s) 295, 393, 483
HinP1I GCGC 3 cut(s) 295, 393, 483
HincII GTYRAC 2 cut(s) 401, 787
HindII GTYRAC 2 cut(s) 401, 787
HindIII AAGCTT 1 cut(s) 605
HinfI GANTC 2 cut(s) 386, 838
HpaI GTTAAC 1 cut(s) 787
HphI GGTGA 1 cut(s) 701
Hpy166II GTNNAC 4 cut(s) 94, 401, 787, 826
Hpy188I TCNGA 1 cut(s) 70
Hpy8I GTNNAC 4 cut(s) 94, 401, 787, 826
HpyAV CCTTC 2 cut(s) 217, 582
HpyCH4III ACNGT 4 cut(s) 179, 361, 668, 737
HpyCH4IV ACGT 1 cut(s) 271
HpyCH4V TGCA 6 cut(s) 59, 194, 421, 497, 651, 733
HpyF10VI GCNNNNNNNGC 1 cut(s) 303
HpyF3I CTNAG 3 cut(s) 81, 603, 625
HpySE526I ACGT 1 cut(s) 271
Hsp92II CATG 5 cut(s) 249, 520, 579, 686, 824
HspAI GCGC 3 cut(s) 295, 393, 483
KspAI GTTAAC 1 cut(s) 787
Kzo9I GATC 1 cut(s) 115
LmnI GCTCC 2 cut(s) 371, 435
LweI GCATC 3 cut(s) 408, 720, 814
MaeI CTAG 4 cut(s) 396, 428, 713, 774
MaeII ACGT 1 cut(s) 271
MaeIII GTNAC 1 cut(s) 210
MalI GATC 1 cut(s) 117
MboI GATC 1 cut(s) 115
MboII GAAGA 5 cut(s) 232, 289, 292, 338, 791
MhlI GDGCHC 2 cut(s) 368, 624
MluCI AATT 5 cut(s) 110, 152, 370, 556, 586
MmeI TCCRAC 1 cut(s) 254
MnlI CCTC 3 cut(s) 541, 691, 766
MroXI GAANNNNTTC 1 cut(s) 110
MseI TTAA 6 cut(s) 126, 216, 522, 552, 786, 810
MslI CAYNNNNRTG 1 cut(s) 515
MspCI CTTAAG 1 cut(s) 215
MspR9I CCNGG 3 cut(s) 376, 545, 830
Mva1269I GAATGC 1 cut(s) 196
MvaI CCWGG 3 cut(s) 376, 545, 830
MwoI GCNNNNNNNGC 1 cut(s) 303
NdeI CATATG 1 cut(s) 143
NdeII GATC 1 cut(s) 115
NlaIII CATG 5 cut(s) 249, 520, 579, 686, 824
NlaIV GGNNCC 1 cut(s) 511
NsbI TGCGCA 1 cut(s) 296
NspI RCATGY 3 cut(s) 249, 520, 824
PciI ACATGT 1 cut(s) 820
PctI GAATGC 1 cut(s) 196
PdmI GAANNNNTTC 1 cut(s) 110
PfeI GAWTC 2 cut(s) 386, 838
PflMI CCANNNNNTGG 1 cut(s) 375
PfoI TCCNGGA 2 cut(s) 374, 543
PscI ACATGT 1 cut(s) 820
Psp124BI GAGCTC 1 cut(s) 368
Psp1406I AACGTT 1 cut(s) 271
Psp6I CCWGG 3 cut(s) 374, 543, 828
PspGI CCWGG 3 cut(s) 374, 543, 828
PspN4I GGNNCC 1 cut(s) 511
PspPI GGNCC 1 cut(s) 40
RseI CAYNNNNRTG 1 cut(s) 515
SacI GAGCTC 1 cut(s) 368
SaqAI TTAA 6 cut(s) 126, 216, 522, 552, 786, 810
Sau3AI GATC 1 cut(s) 115
Sau96I GGNCC 1 cut(s) 40
ScrFI CCNGG 3 cut(s) 376, 545, 830
SduI GDGCHC 2 cut(s) 368, 624
SetI ASST 8 cut(s) 212, 274, 368, 515, 528, 609, 714, 758
SfaNI GCATC 3 cut(s) 408, 720, 814
SmiMI CAYNNNNRTG 1 cut(s) 515
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
SpeI ACTAGT 1 cut(s) 427
Sse9I AATT 5 cut(s) 110, 152, 370, 556, 586
SsiI CCGC 1 cut(s) 747
SspMI CTAG 4 cut(s) 396, 428, 713, 774
SstI GAGCTC 1 cut(s) 368
StyD4I CCNGG 3 cut(s) 374, 543, 828
TaaI ACNGT 4 cut(s) 179, 361, 668, 737
TaiI ACGT 1 cut(s) 274
TaqI TCGA 2 cut(s) 114, 851
TaqII GACCGA 1 cut(s) 328
TasI AATT 5 cut(s) 110, 152, 370, 556, 586
TfiI GAWTC 2 cut(s) 386, 838
Tru1I TTAA 6 cut(s) 126, 216, 522, 552, 786, 810
Tru9I TTAA 6 cut(s) 126, 216, 522, 552, 786, 810
TscAI CASTG 3 cut(s) 184, 366, 740
TspDTI ATGAA 3 cut(s) 242, 282, 564
TspRI CASTG 3 cut(s) 184, 366, 740
Van91I CCANNNNNTGG 1 cut(s) 375
Vha464I CTTAAG 1 cut(s) 215
XapI RAATTY 3 cut(s) 110, 152, 586
XceI RCATGY 3 cut(s) 249, 520, 824
XmnI GAANNNNTTC 1 cut(s) 110
XspI CTAG 4 cut(s) 396, 428, 713, 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.