Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0024604

Physical Location & Seq

Genomic Coordinates Forward (+)

13865407 .. 13870177

Gene Structure 4771 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3270 bp

ATGGCATCTACTACTAGGAAATTCATGGTATCTATTTGCAAGAAGCTATCACTCAAGTTGAAAACATGGCATATACACAACTTGCATCAAATTTACCAAGTAAAAGTCAAGATGGTAAATTCACTTGAAAATATAAGCATTCATAATCATGGCTACATTGTAGCCCTATGTATTCATAATAAAAATGAGAAGAAAATTGAGTTAAGAAAAAACGCTGAAAAATCCTTGTGCTCTGCTATTAATCAGTTCTCTTTTCTTTTTTTTTCTTCCCACTTCTCCTTCCTTTTCCGTAACCGACAAAGAAACTTCAACTCCTTCTCGTTGATATCTCACTGCCATAACCCCTACACATCTCCCAAATGGACATCCACCAGATTTGCAACTTGGCCAATCTCCATAACAGATCAAAATGATCAAATAAATATCTCATTTGGGTTTCCATCTCTTTTTGAATTTGATCACCCTAAAACCCTTGCTCTGCTGCAATTGTATGTGGCGGTTTGCTCCAGAAAAATTGGGTTAGGCCTTTCTTTCCTCTGTCGCATGTGCAAGGCATGTCTTCCACATTTTGTTGACTTGTCAAACCTCCTTTTAGACTTTAAAAACAATATGATCCCTGCACAAAAATTCAACCAAATAAACTGCTGTCTAATTTCTAAAGGAATGATCTCTACTTCACTTGAATTGAACTTAAAAGGATTGAATTCATATTTTTCTCAAAACCATGTAATTTCATTCATTTTGAACAAACATAACACAAGAAAATGTAGCTTTCGGAGTAAAATAAGTAGTAAAATATGTTCTAGAATAATACCATTGTTTGGTTTTGCACCAAGTCTTGGATTAGCTCCAAGAACTTTGTTTTTTCTTGAAGTCTCTGTTCACGTCTTCCTAGGTCATCCTCTACTTCTTTTCCCTAACCTTCTTCAATTGTCGATCCTCCTACTACTTACTTTAGATATCCTTATTTTTAATTGTGTGCCCACACATCCAATGAAGAATTCTCATTTCCGCCACACTCATTTTTTGCACGAGTTGATGCTTGACCGCCTAACATTCCATTTTGTGGTTTACGATGGTTGCACAACACACCGATGCACTATTTCACTTCATCCATCTAGCTTATATTATATGGTTGAGATCTCCATCCAAAGACACACAAACTCTCTGTCGTGCTGTCAAAATGCAGCATATCTTGATGCTTCTCTGTTGGGATTATTCTTGATTTTGAAGATTTCCTATGTTTCTATGTCCTTTCAGTTTGTAACTGGTCTTCATTTTCAACACTATATTGCAGGTAGACATGATGGTCCTCACATGATCGTCCTCACAGATGGCAGTCGTATCCTTGGCCTAGGGGACCTTGGAGTTCAGGGAATTGGAATTCCATTGGAACTCTTAATATATATGTTTGCTGCAGCTAATATTAATCCACAAAGAGTATGCTCTCTCTCTCTCTCTCTGAGTCTACATCTTACTAATCTAGTAGATCTTATATCGCTTCACCTATATACCTCTATGACAGATACTACCGGCTATACTATCGATTTGAGCACACAACTTCTCATGGCTCACCAGAAGCATCTCGATCACATGCATCATCATGATTTGTTTGGTTATATTTTTCAACCAACAAGTAATTGTGTACAAAAAGATGGTTTTCATCCATTAAAATCGGAATTCAAGTCGATGTATTTTTTAGTTGTGCTTATATATATTCACCATAGTTTTGTGGCTTTGGATTCAAATAATGTCATGAATCACTGTTGCCTGCTTGAAACATTACAAATAAATAATTTCCAAACTTTGAAATCCCCAGAAGTGAAGATTGAAGATTTCAAGTCTGATTTAAATGACAGGCACTTGTACTCAAGCATTGAGAATACTTATAAATTAAACTTGATAATTTTGATTATAGTAATTACTCATATCATTAAGGGCTATTCAATCACTGGAAAGATACAAGATCCATTTTTGAGAGCCACTGAAAAATGTGGAATAAGGAAACCTATAGTAAACCCAAATGGTATTCTTGGGTGCATAGACATAGTTTGCTATTGTAGGAGCAACCACATTTTTATAAGTGCAGTAATACTTGAGATTTTAAGTTTTGATCTCTCTCTCTCTCTCTCTCTCTGCAATTTCATAAAGAATAGAGAGTGCCATGAAATTCCTTATCAAAGATTTCAAATGAAGAGCATAAAGCTCAAAATATTGTTGCAACATACCCTTAAAAGAGTTAAAAGTTTTAGTATGACTCTTGAAGAGCCTTCAAGGATATTTGTATATATAATTTACATTTACCATGGAGCTTTATACTACTCTTTGTTTATTTTAATTCCTAGTTTTTATTTTGTATTTGGTCTTTGTTGGAACACTAATGGTTGTTGTCAAATGCCAATAAACTTCTGCACTTCCCTAGGTCAAGCCCTAACCAAACTTAATACTGAAGCACATTACTTCACACTTGACAATGATGACAATTATTCATCAAAGGGAATCTTCGACAGTATGCTATACAAGAGCTTTGTGCTCTTTTCCCTTCGACTAGGTTTTTATCCCACTAGGTTTTTCCTGTCAAAATTAATTTTAGAGAGGCAACATTATGCGCGTCCAAAACCTTATCAATGTTTTCTAAGTTGTTTTCCAAGCAAGGAGGAGTGTTGCAAATATTATTCATTATATAGCTATTCACCTTCCGGGTCAATATGTCATCCCAATAGTTCAACACTTTTCTACGTAAATCCTACGTCTATAATAGGCACCCTATTAAGAAGGAAATGGCACCTTGATTTCTCCACCAATTTCTCTTTTGTCTCTCTACATTCTCTCTTTCGCTATTATATAAATATTAATTCTCTTTGTTTACACCTTTATTTTAATCTTTTATATAGTGGAAAAGAAGCTAAAAAGAAAGTATTGAGACTAAAAATAGTTTGCAATTCCATGTCGTCAGCACAGCCTCTACCGATCAGTCGAGTTCGTGGATTAAATTGTATGACTCGGGAAGAACCAGGAGATTTAACATATATTTCTGGAAAGCTACGGATGTTTATTTTCTATAGAATTTTATGGTTTGGGAATAGGAGGAAGAGGAAAGACCTAAGCATCGTTCTACAATTTATCATTGAGGAGTTTCTTCTTCTCCTTTTTATTTTCTGTTTTGCATGGCTTTTATTTTCATTATACTTTGTTACAAAACAATCTATTGATGCTTTAATATTTAATTCCTATTTTAACTCCAATCTTATTGAGTTATTTTATTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004470 GO:0004471 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006090 GO:0006108 GO:0008144 GO:0008150 GO:0008152 GO:0008270 GO:0009507 GO:0009536 GO:0009987 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0017076 GO:0019752 GO:0030554 GO:0031974 GO:0032553 GO:0032555 GO:0032559 GO:0032787 GO:0035639 GO:0036094 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043648 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046914 GO:0046983 GO:0050897 GO:0055114 GO:0070013 GO:0071704 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

1090

Amino Acids

126.05

Weight (kDa)

9.19

Isoelectric Point (pI)

38.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
malic	PF00390	436 - 502	1.1e-10	Malic enzyme, N-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0024604)

Species	Orthologous Gene IDs
pyrus_communis	pycom01g03150 pycom07g12520 pycom07g12590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1890, 2081
AasI	GACNNNNNNGTC	1 cut(s)	1308
Acc36I	ACCTGC	1 cut(s)	1286
AccB1I	GGYRCC	2 cut(s)	2762, 2784
AccB7I	CCANNNNNTGG	3 cut(s)	821, 839, 1066
AccI	GTMKAC	2 cut(s)	1300, 1468
AccII	CGCG	1 cut(s)	2611
AciI	CCGC	3 cut(s)	497, 1012, 1048
AclWI	GGATC	3 cut(s)	607, 931, 1961
AcoI	YGGCCR	1 cut(s)	386
AcuI	CTGAAG	1 cut(s)	2469
AfaI	GTAC	2 cut(s)	1647, 1868
AfiI	CCNNNNNNNGG	3 cut(s)	821, 839, 1066
AjiI	CACGTC	1 cut(s)	886
AjnI	CCWGG	1 cut(s)	3013
AjuI	GAANNNNNNNTTGG	2 cut(s)	985, 1017
Alw21I	GWGCWC	3 cut(s)	233, 1556, 2535
Alw26I	GTCTC	3 cut(s)	880, 2821, 2917
AlwI	GGATC	3 cut(s)	607, 931, 1961
Ama87I	CYCGRG	1 cut(s)	3003
AoxI	GGCC	3 cut(s)	386, 523, 1351
ApeKI	GCWGC	4 cut(s)	481, 1187, 1415, 1418
ArsI	GACNNNNNNTTYG	2 cut(s)	1154, 1186
AseI	ATTAAT	4 cut(s)	240, 1428, 2585, 2853
AspA2I	CCTAGG	3 cut(s)	892, 1354, 2419
AspLEI	GCGC	1 cut(s)	2611
AspS9I	GGNCC	2 cut(s)	1310, 1360
AsuC2I	CCSGG	1 cut(s)	2701
AsuHPI	GGTGA	5 cut(s)	452, 1496, 1565, 1712, 2685
AvaI	CYCGRG	1 cut(s)	3003
AvaII	GGWCC	2 cut(s)	1310, 1360
AvrII	CCTAGG	3 cut(s)	892, 1354, 2419
BaeGI	GKGCMC	1 cut(s)	984
BalI	TGGCCA	1 cut(s)	388
BanI	GGYRCC	2 cut(s)	2762, 2784
BarI	GAAGNNNNNNTAC	2 cut(s)	1257, 1289
BauI	CACGAG	1 cut(s)	1031
BbsI	GAAGAC	3 cut(s)	551, 880, 1265
Bbv12I	GWGCWC	3 cut(s)	233, 1556, 2535
BbvI	GCAGC	4 cut(s)	468, 1199, 1402, 1430
BccI	CCATC	8 cut(s)	106, 448, 1070, 1123, 1154, 1301, 1328, 1649
BciT130I	CCWGG	1 cut(s)	3015
BciVI	GTATCC	1 cut(s)	1355
BclI	TGATCA	2 cut(s)	412, 457
BcnI	CCSGG	1 cut(s)	2701
BcoDI	GTCTC	3 cut(s)	880, 2821, 2917
BfmI	CTRYAG	3 cut(s)	1416, 2010, 3061
BfuAI	ACCTGC	1 cut(s)	1286
BfuI	GTATCC	1 cut(s)	1355
BglII	AGATCT	2 cut(s)	1140, 1489
BisI	GCNGC	4 cut(s)	482, 1188, 1416, 1419
BlnI	CCTAGG	3 cut(s)	892, 1354, 2419
BlsI	GCNGC	4 cut(s)	483, 1189, 1417, 1420
Bme1390I	CCNGG	2 cut(s)	2701, 3015
Bme18I	GGWCC	2 cut(s)	1310, 1360
BmeT110I	CYCGRG	1 cut(s)	3003
BmgBI	CACGTC	1 cut(s)	886
BmgT120I	GGNCC	2 cut(s)	1310, 1360
BmiI	GGNNCC	3 cut(s)	1361, 2764, 2786
BmrFI	CCNGG	2 cut(s)	2701, 3015
BmsI	GCATC	9 cut(s)	14, 94, 1029, 1085, 1189, 1591, 1606, 3117, 3202
BpiI	GAAGAC	3 cut(s)	551, 880, 1265
BpmI	CTGGAG	1 cut(s)	490
Bpu10I	CCTNAGC	1 cut(s)	3104
BpuEI	CTTGAG	3 cut(s)	38, 1855, 2117
BpuMI	CCSGG	1 cut(s)	2701
Bsa29I	ATCGAT	1 cut(s)	1545
BsaAI	YACGTR	1 cut(s)	2740
BsaBI	GATNNNNATC	1 cut(s)	1145
BsaJI	CCNNGG	6 cut(s)	892, 1348, 1354, 1363, 2305, 2419
BsaXI	ACNNNNNCTCC	2 cut(s)	296, 326
Bsc4I	CCNNNNNNNGG	3 cut(s)	821, 839, 1066
Bse118I	RCCGGY	1 cut(s)	1532
Bse1I	ACTGG	2 cut(s)	1273, 1957
Bse8I	GATNNNNATC	1 cut(s)	1145
BseBI	CCWGG	1 cut(s)	3015
BseCI	ATCGAT	1 cut(s)	1545
BseDI	CCNNGG	6 cut(s)	892, 1348, 1354, 1363, 2305, 2419
BseGI	GGATG	8 cut(s)	365, 898, 988, 1111, 1146, 1663, 2713, 3054
BseJI	GATNNNNATC	1 cut(s)	1145
BseLI	CCNNNNNNNGG	3 cut(s)	821, 839, 1066
BseMII	CTCAG	1 cut(s)	1454
BseNI	ACTGG	2 cut(s)	1273, 1957
BseRI	GAGGAG	2 cut(s)	2672, 3146
BseSI	GKGCMC	1 cut(s)	984
BseXI	GCAGC	4 cut(s)	468, 1199, 1402, 1430
BsgI	GTGCAG	3 cut(s)	603, 2106, 2395
Bsh1236I	CGCG	1 cut(s)	2611
BshFI	GGCC	3 cut(s)	388, 525, 1353
BshNI	GGYRCC	2 cut(s)	2762, 2784
BshVI	ATCGAT	1 cut(s)	1545
BsiHKAI	GWGCWC	3 cut(s)	233, 1556, 2535
BsiHKCI	CYCGRG	1 cut(s)	3003
BsiSI	CCGG	2 cut(s)	1533, 2700
BslFI	GGGAC	1 cut(s)	1373
BslI	CCNNNNNNNGG	3 cut(s)	821, 839, 1066
BsmAI	GTCTC	3 cut(s)	880, 2821, 2917
BsmFI	GGGAC	1 cut(s)	1373
BsmI	GAATGC	1 cut(s)	138
BsnI	GGCC	3 cut(s)	388, 525, 1353
BsoBI	CYCGRG	1 cut(s)	3003
Bsp1286I	GDGCHC	4 cut(s)	233, 984, 1556, 2535
Bsp1407I	TGTACA	1 cut(s)	1645
Bsp19I	CCATGG	1 cut(s)	2305
BspACI	CCGC	3 cut(s)	497, 1012, 1048
BspANI	GGCC	3 cut(s)	388, 525, 1353
BspCNI	CTCAG	1 cut(s)	1455
BspDI	ATCGAT	1 cut(s)	1545
BspFNI	CGCG	1 cut(s)	2611
BspHI	TCATGA	2 cut(s)	1603, 1755
BspLI	GGNNCC	3 cut(s)	1361, 2764, 2786
BspMAI	CTGCAG	1 cut(s)	1420
BspMI	ACCTGC	1 cut(s)	1286
BspPI	GGATC	3 cut(s)	607, 931, 1961
BspQI	GCTCTTC	2 cut(s)	2189, 2259
BspT107I	GGYRCC	2 cut(s)	2762, 2784
BsrFI	RCCGGY	1 cut(s)	1532
BsrGI	TGTACA	1 cut(s)	1645
BsrI	ACTGG	2 cut(s)	1273, 1957
BssAI	RCCGGY	1 cut(s)	1532
BssECI	CCNNGG	6 cut(s)	892, 1348, 1354, 1363, 2305, 2419
BssSI	CACGAG	1 cut(s)	1031
BssT1I	CCWWGG	6 cut(s)	892, 1348, 1354, 1363, 2305, 2419
Bst2BI	CACGAG	1 cut(s)	1031
Bst2UI	CCWGG	1 cut(s)	3015
Bst4CI	ACNGT	2 cut(s)	1766, 2510
Bst6I	CTCTTC	3 cut(s)	2189, 2259, 3086
BstAUI	TGTACA	1 cut(s)	1645
BstBAI	YACGTR	1 cut(s)	2740
BstC8I	GCNNGC	1 cut(s)	1772
BstDEI	CTNAG	3 cut(s)	1463, 2636, 3104
BstDSI	CCRYGG	1 cut(s)	2305
BstF5I	GGATG	8 cut(s)	365, 898, 988, 1111, 1146, 1663, 2713, 3054
BstFNI	CGCG	1 cut(s)	2611
BstHHI	GCGC	1 cut(s)	2611
BstMAI	GTCTC	3 cut(s)	880, 2821, 2917
BstNI	CCWGG	1 cut(s)	3015
BstNSI	RCATGY	3 cut(s)	547, 558, 1597
BstSCI	CCNGG	2 cut(s)	2699, 3013
BstSFI	CTRYAG	3 cut(s)	1416, 2010, 3061
BstSLI	GKGCMC	1 cut(s)	984
BstSNI	TACGTA	1 cut(s)	2740
BstUI	CGCG	1 cut(s)	2611
BstV1I	GCAGC	4 cut(s)	468, 1199, 1402, 1430
BstV2I	GAAGAC	3 cut(s)	551, 880, 1265
BstX2I	RGATCY	3 cut(s)	1140, 1489, 1966
BstYI	RGATCY	3 cut(s)	1140, 1489, 1966
Bsu15I	ATCGAT	1 cut(s)	1545
BsuI	GTATCC	1 cut(s)	1355
BsuRI	GGCC	3 cut(s)	388, 525, 1353
BsuTUI	ATCGAT	1 cut(s)	1545
BtgI	CCRYGG	1 cut(s)	2305
BtrI	CACGTC	1 cut(s)	886
BtsCI	GGATG	8 cut(s)	365, 898, 988, 1111, 1146, 1663, 2713, 3054
BtsI	GCAGTG	1 cut(s)	331
BtsIMutI	CAGTG	4 cut(s)	331, 1762, 1950, 1983
BveI	ACCTGC	1 cut(s)	1286
Cac8I	GCNNGC	1 cut(s)	1772
CciI	TCATGA	2 cut(s)	1603, 1755
CfoI	GCGC	1 cut(s)	2611
Cfr10I	RCCGGY	1 cut(s)	1532
Cfr13I	GGNCC	2 cut(s)	1310, 1360
ClaI	ATCGAT	1 cut(s)	1545
CseI	GACGC	1 cut(s)	2600
Csp6I	GTAC	2 cut(s)	1646, 1867
CspCI	CAANNNNNGTGG	2 cut(s)	358, 393
CviQI	GTAC	2 cut(s)	1646, 1867
DdeI	CTNAG	3 cut(s)	1463, 2636, 3104
DraI	TTTAAA	2 cut(s)	601, 1851
DrdI	GACNNNNNNGTC	1 cut(s)	1308
DseDI	GACNNNNNNGTC	1 cut(s)	1308
EaeI	YGGCCR	1 cut(s)	386
Eam1104I	CTCTTC	3 cut(s)	2189, 2259, 3086
EarI	CTCTTC	3 cut(s)	2189, 2259, 3086
EciI	GGCGGA	1 cut(s)	1001
Eco105I	TACGTA	1 cut(s)	2740
Eco130I	CCWWGG	6 cut(s)	892, 1348, 1354, 1363, 2305, 2419
Eco147I	AGGCCT	1 cut(s)	525
Eco32I	GATATC	2 cut(s)	327, 961
Eco47I	GGWCC	2 cut(s)	1310, 1360
Eco57I	CTGAAG	1 cut(s)	2469
Eco88I	CYCGRG	1 cut(s)	3003
EcoO109I	RGGNCCY	1 cut(s)	1360
EcoRI	GAATTC	4 cut(s)	703, 1000, 1383, 1679
EcoRII	CCWGG	1 cut(s)	3013
EcoRV	GATATC	2 cut(s)	327, 961
EcoT14I	CCWWGG	6 cut(s)	892, 1348, 1354, 1363, 2305, 2419
EcoT22I	ATGCAT	1 cut(s)	1599
ErhI	CCWWGG	6 cut(s)	892, 1348, 1354, 1363, 2305, 2419
FalI	AAGNNNNNCTT	2 cut(s)	2486, 2518
FaqI	GGGAC	1 cut(s)	1373
FbaI	TGATCA	2 cut(s)	412, 457
FblI	GTMKAC	2 cut(s)	1300, 1468
Fnu4HI	GCNGC	4 cut(s)	482, 1188, 1416, 1419
FokI	GGATG	8 cut(s)	352, 885, 975, 1098, 1133, 1650, 2700, 3061
Fsp4HI	GCNGC	4 cut(s)	482, 1188, 1416, 1419
GlaI	GCGC	1 cut(s)	2610
GluI	GCNGC	4 cut(s)	482, 1188, 1416, 1419
GsuI	CTGGAG	1 cut(s)	490
HaeIII	GGCC	3 cut(s)	388, 525, 1353
HapII	CCGG	2 cut(s)	1533, 2700
HgaI	GACGC	1 cut(s)	2600
HhaI	GCGC	1 cut(s)	2611
Hin6I	GCGC	1 cut(s)	2609
HinP1I	GCGC	1 cut(s)	2609
HincII	GTYRAC	1 cut(s)	574
HindII	GTYRAC	1 cut(s)	574
HinfI	GANTC	6 cut(s)	1465, 1742, 1759, 2257, 2499, 3001
HpaII	CCGG	2 cut(s)	1533, 2700
HphI	GGTGA	5 cut(s)	452, 1496, 1565, 1712, 2685
Hpy166II	GTNNAC	8 cut(s)	574, 883, 1072, 1301, 1469, 1646, 2017, 2867
Hpy188I	TCNGA	4 cut(s)	777, 1464, 1678, 1846
Hpy8I	GTNNAC	8 cut(s)	574, 883, 1072, 1301, 1469, 1646, 2017, 2867
HpyAV	CCTTC	7 cut(s)	289, 325, 932, 2280, 2552, 2706, 2769
HpyCH4III	ACNGT	2 cut(s)	1766, 2510
HpyCH4IV	ACGT	3 cut(s)	885, 2739, 2750
HpyF3I	CTNAG	3 cut(s)	1463, 2636, 3104
HpySE526I	ACGT	3 cut(s)	885, 2739, 2750
HspAI	GCGC	1 cut(s)	2609
Ksp22I	TGATCA	2 cut(s)	412, 457
LguI	GCTCTTC	2 cut(s)	2189, 2259
LmnI	GCTCC	4 cut(s)	509, 853, 2064, 2309
Lsp1109I	GCAGC	4 cut(s)	468, 1199, 1402, 1430
LweI	GCATC	9 cut(s)	14, 94, 1029, 1085, 1189, 1591, 1606, 3117, 3202
MaeII	ACGT	3 cut(s)	885, 2739, 2750
MaeIII	GTNAC	3 cut(s)	290, 1264, 3193
MfeI	CAATTG	2 cut(s)	485, 929
MflI	RGATCY	3 cut(s)	1140, 1489, 1966
MhlI	GDGCHC	4 cut(s)	233, 984, 1556, 2535
MlsI	TGGCCA	1 cut(s)	388
MluNI	TGGCCA	1 cut(s)	388
MlyI	GAGTC	3 cut(s)	1474, 2251, 2995
MmeI	TCCRAC	1 cut(s)	2351
Mox20I	TGGCCA	1 cut(s)	388
Mph1103I	ATGCAT	1 cut(s)	1599
MscI	TGGCCA	1 cut(s)	388
MslI	CAYNNNNRTG	3 cut(s)	147, 1093, 1602
Msp20I	TGGCCA	1 cut(s)	388
MspI	CCGG	2 cut(s)	1533, 2700
MspR9I	CCNGG	2 cut(s)	2701, 3015
MunI	CAATTG	2 cut(s)	485, 929
Mva1269I	GAATGC	1 cut(s)	138
MvaI	CCWGG	1 cut(s)	3015
MvnI	CGCG	1 cut(s)	2611
NciI	CCSGG	1 cut(s)	2701
NcoI	CCATGG	1 cut(s)	2305
NlaIV	GGNNCC	3 cut(s)	1361, 2764, 2786
NsiI	ATGCAT	1 cut(s)	1599
NspI	RCATGY	3 cut(s)	547, 558, 1597
PagI	TCATGA	2 cut(s)	1603, 1755
PceI	AGGCCT	1 cut(s)	525
PciSI	GCTCTTC	2 cut(s)	2189, 2259
PctI	GAATGC	1 cut(s)	138
PfeI	GAWTC	3 cut(s)	1742, 1759, 2499
PflMI	CCANNNNNTGG	3 cut(s)	821, 839, 1066
PkrI	GCNGC	4 cut(s)	483, 1189, 1417, 1420
PleI	GAGTC	3 cut(s)	1473, 2251, 2995
PpsI	GAGTC	3 cut(s)	1473, 2251, 2995
Ppu21I	YACGTR	1 cut(s)	2740
PpuMI	RGGWCCY	1 cut(s)	1360
PshBI	ATTAAT	4 cut(s)	240, 1428, 2585, 2853
PsiI	TTATAA	2 cut(s)	1890, 2081
Psp5II	RGGWCCY	1 cut(s)	1360
Psp6I	CCWGG	1 cut(s)	3013
PspGI	CCWGG	1 cut(s)	3013
PspN4I	GGNNCC	3 cut(s)	1361, 2764, 2786
PspPI	GGNCC	2 cut(s)	1310, 1360
PspPPI	RGGWCCY	1 cut(s)	1360
PsrI	GAACNNNNNNTAC	2 cut(s)	784, 816
PstI	CTGCAG	1 cut(s)	1420
PsuI	RGATCY	3 cut(s)	1140, 1489, 1966
RsaI	GTAC	2 cut(s)	1647, 1868
RsaNI	GTAC	2 cut(s)	1646, 1867
RseI	CAYNNNNRTG	3 cut(s)	147, 1093, 1602
SapI	GCTCTTC	2 cut(s)	2189, 2259
SatI	GCNGC	4 cut(s)	482, 1188, 1416, 1419
Sau96I	GGNCC	2 cut(s)	1310, 1360
SchI	GAGTC	3 cut(s)	1474, 2251, 2995
ScrFI	CCNGG	2 cut(s)	2701, 3015
SduI	GDGCHC	4 cut(s)	233, 984, 1556, 2535
SfaNI	GCATC	9 cut(s)	14, 94, 1029, 1085, 1189, 1591, 1606, 3117, 3202
SfcI	CTRYAG	3 cut(s)	1416, 2010, 3061
SinI	GGWCC	2 cut(s)	1310, 1360
SmiI	ATTTAAAT	1 cut(s)	1851
SmiMI	CAYNNNNRTG	3 cut(s)	147, 1093, 1602
SmlI	CTYRAG	3 cut(s)	53, 1870, 2096
SmoI	CTYRAG	3 cut(s)	53, 1870, 2096
SnaBI	TACGTA	1 cut(s)	2740
SseBI	AGGCCT	1 cut(s)	525
SsiI	CCGC	3 cut(s)	497, 1012, 1048
SspI	AATATT	5 cut(s)	1426, 2214, 2672, 2851, 3222
StuI	AGGCCT	1 cut(s)	525
StyD4I	CCNGG	2 cut(s)	2699, 3013
StyI	CCWWGG	6 cut(s)	892, 1348, 1354, 1363, 2305, 2419
SwaI	ATTTAAAT	1 cut(s)	1851
TaaI	ACNGT	2 cut(s)	1766, 2510
TaiI	ACGT	3 cut(s)	888, 2742, 2753
TaqI	TCGA	7 cut(s)	935, 1545, 1587, 1688, 2505, 2545, 2977
TatI	WGTACW	2 cut(s)	1645, 1866
TfiI	GAWTC	3 cut(s)	1742, 1759, 2499
TscAI	CASTG	4 cut(s)	338, 1769, 1957, 1990
TseI	GCWGC	4 cut(s)	481, 1187, 1415, 1418
TspGWI	ACGGA	2 cut(s)	278, 3061
TspRI	CASTG	4 cut(s)	338, 1769, 1957, 1990
Van91I	CCANNNNNTGG	3 cut(s)	821, 839, 1066
VpaK11BI	GGWCC	2 cut(s)	1310, 1360
VspI	ATTAAT	4 cut(s)	240, 1428, 2585, 2853
XbaI	TCTAGA	1 cut(s)	803
XceI	RCATGY	3 cut(s)	547, 558, 1597
XmaJI	CCTAGG	3 cut(s)	892, 1354, 2419
XmiI	GTMKAC	2 cut(s)	1300, 1468
Zsp2I	ATGCAT	1 cut(s)	1599

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.