pycom07g16550

Pumilio homolog 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0021164
Physical Location & Seq
Forward (+)
18972626 .. 18974372
1747 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 285 bp
ATGTATGGTTGTAGAGTGATCCAGAAGGCTTTGGAAGCAGTTGATGTGAATCAGCAGGCACAGATGGTGTCGGAGCTTGATGGTTCAATCATGAAATGTGTTCGTGATCGATCAGAATGGGAATCATGTCATTCAGAAGTGTATAGAATGCATCCCTCAAGACAAAATCCAATTTATAATTTCATCCTTCTATGGGCAAGTTGTCGCACTATCCACTCATCCTTATGGTTGCCGGGTTATACAGTCGGCAGAAGTTTGCTTCTAATGTTGTGGAGAAGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0071944 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

95

Amino Acids

10.95

Weight (kDa)

8.45

Isoelectric Point (pI)

60.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 1 - 26 2.3e-07 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021164)

Species Orthologous Gene IDs
malus_domestica MD16G1252900.v1.1
pyrus_communis pycom07g16550
rosa_multiflora Rmu_sc0037811.1_g000001 Rmu_sc0039367.1_g000001 Rmu_sc0042890.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
AclWI GGATC 1 cut(s) 13
AfiI CCNNNNNNNGG 1 cut(s) 193
AgsI TTSAA 1 cut(s) 87
AluBI AGCT 1 cut(s) 76
AluI AGCT 1 cut(s) 76
AlwI GGATC 1 cut(s) 13
AsuC2I CCSGG 1 cut(s) 234
BccI CCATC 2 cut(s) 58, 74
BcnI CCSGG 1 cut(s) 234
Bme1390I CCNGG 1 cut(s) 234
BmrFI CCNGG 1 cut(s) 234
BmsI GCATC 1 cut(s) 160
BpuEI CTTGAG 1 cut(s) 142
BpuMI CCSGG 1 cut(s) 234
Bsa29I ATCGAT 1 cut(s) 109
BsaBI GATNNNNATC 1 cut(s) 48
Bsc4I CCNNNNNNNGG 1 cut(s) 193
Bse8I GATNNNNATC 1 cut(s) 48
BseCI ATCGAT 1 cut(s) 109
BseGI GGATG 3 cut(s) 151, 183, 218
BseJI GATNNNNATC 1 cut(s) 48
BseLI CCNNNNNNNGG 1 cut(s) 193
BshVI ATCGAT 1 cut(s) 109
BsiSI CCGG 1 cut(s) 233
BslI CCNNNNNNNGG 1 cut(s) 193
BsmI GAATGC 1 cut(s) 153
Bsp143I GATC 3 cut(s) 18, 106, 110
BspDI ATCGAT 1 cut(s) 109
BspHI TCATGA 1 cut(s) 90
BspPI GGATC 1 cut(s) 13
BssMI GATC 3 cut(s) 18, 106, 110
Bst4CI ACNGT 1 cut(s) 244
BstC8I GCNNGC 1 cut(s) 57
BstF5I GGATG 3 cut(s) 151, 183, 218
BstKTI GATC 3 cut(s) 21, 109, 113
BstMBI GATC 3 cut(s) 18, 106, 110
BstMWI GCNNNNNNNGC 1 cut(s) 35
BstSCI CCNGG 1 cut(s) 232
Bsu15I ATCGAT 1 cut(s) 109
BsuTUI ATCGAT 1 cut(s) 109
BtsCI GGATG 3 cut(s) 151, 183, 218
Cac8I GCNNGC 1 cut(s) 57
CciI TCATGA 1 cut(s) 90
ClaI ATCGAT 1 cut(s) 109
CviAII CATG 2 cut(s) 91, 126
CviJI RGCY 2 cut(s) 29, 76
CviKI_1 RGCY 2 cut(s) 29, 76
DpnI GATC 3 cut(s) 20, 108, 112
DpnII GATC 3 cut(s) 18, 106, 110
EcoT22I ATGCAT 1 cut(s) 153
FaeI CATG 2 cut(s) 94, 129
FaiI YATR 8 cut(s) 6, 92, 127, 144, 177, 193, 226, 240
FatI CATG 2 cut(s) 90, 125
FokI GGATG 3 cut(s) 138, 170, 205
HapII CCGG 1 cut(s) 233
Hin1II CATG 2 cut(s) 94, 129
HinfI GANTC 2 cut(s) 49, 122
HpaII CCGG 1 cut(s) 233
Hpy188I TCNGA 3 cut(s) 73, 115, 136
Hpy188III TCNNGA 4 cut(s) 22, 91, 104, 159
HpyAV CCTTC 2 cut(s) 19, 197
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4V TGCA 1 cut(s) 151
HpyF10VI GCNNNNNNNGC 1 cut(s) 35
Hsp92II CATG 2 cut(s) 94, 129
Kzo9I GATC 3 cut(s) 18, 106, 110
LmnI GCTCC 1 cut(s) 73
LpnPI CCDG 3 cut(s) 35, 41, 246
LweI GCATC 1 cut(s) 160
MalI GATC 3 cut(s) 20, 108, 112
MboI GATC 3 cut(s) 18, 106, 110
MluCI AATT 2 cut(s) 171, 178
MmeI TCCRAC 1 cut(s) 51
MnlI CCTC 1 cut(s) 166
Mph1103I ATGCAT 1 cut(s) 153
MseI TTAA 1 cut(s) 283
MslI CAYNNNNRTG 1 cut(s) 223
MspI CCGG 1 cut(s) 233
MspR9I CCNGG 1 cut(s) 234
Mva1269I GAATGC 1 cut(s) 153
MwoI GCNNNNNNNGC 1 cut(s) 35
NciI CCSGG 1 cut(s) 234
NdeII GATC 3 cut(s) 18, 106, 110
NlaIII CATG 2 cut(s) 94, 129
NsiI ATGCAT 1 cut(s) 153
PagI TCATGA 1 cut(s) 90
PctI GAATGC 1 cut(s) 153
PfeI GAWTC 2 cut(s) 49, 122
PsiI TTATAA 1 cut(s) 177
RseI CAYNNNNRTG 1 cut(s) 223
SaqAI TTAA 1 cut(s) 283
Sau3AI GATC 3 cut(s) 18, 106, 110
ScrFI CCNGG 1 cut(s) 234
SetI ASST 1 cut(s) 78
SfaNI GCATC 1 cut(s) 160
SmiMI CAYNNNNRTG 1 cut(s) 223
SmlI CTYRAG 1 cut(s) 157
SmoI CTYRAG 1 cut(s) 157
Sse9I AATT 2 cut(s) 171, 178
StyD4I CCNGG 1 cut(s) 232
TaaI ACNGT 1 cut(s) 244
TaqI TCGA 1 cut(s) 109
TasI AATT 2 cut(s) 171, 178
TfiI GAWTC 2 cut(s) 49, 122
Tru1I TTAA 1 cut(s) 283
Tru9I TTAA 1 cut(s) 283
TspDTI ATGAA 2 cut(s) 107, 172
Zsp2I ATGCAT 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.